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WO2012036810A8 - Thermophilic mutants of trichoderma reesei endoglucanase i - Google Patents

Thermophilic mutants of trichoderma reesei endoglucanase i Download PDF

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Publication number
WO2012036810A8
WO2012036810A8 PCT/US2011/047361 US2011047361W WO2012036810A8 WO 2012036810 A8 WO2012036810 A8 WO 2012036810A8 US 2011047361 W US2011047361 W US 2011047361W WO 2012036810 A8 WO2012036810 A8 WO 2012036810A8
Authority
WO
WIPO (PCT)
Prior art keywords
trichoderma reesei
thermophilic
mutants
reesei endoglucanase
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/047361
Other languages
French (fr)
Other versions
WO2012036810A3 (en
WO2012036810A2 (en
Inventor
Harshal Akshay CHOKHAWALA
Tae-Wan Kim
Harvey W. Blanch
Douglas S. Clark
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CN2011800438167A priority Critical patent/CN103119159A/en
Priority to JP2013529155A priority patent/JP2013539971A/en
Priority to EP11825612.2A priority patent/EP2616542A4/en
Priority to CA2811206A priority patent/CA2811206A1/en
Priority to BR112013006315A priority patent/BR112013006315A2/en
Priority to US13/822,623 priority patent/US9410135B2/en
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Priority to AU2011302536A priority patent/AU2011302536A1/en
Publication of WO2012036810A2 publication Critical patent/WO2012036810A2/en
Publication of WO2012036810A3 publication Critical patent/WO2012036810A3/en
Anticipated expiration legal-status Critical
Publication of WO2012036810A8 publication Critical patent/WO2012036810A8/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/244—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01006—Endo-1,3(4)-beta-glucanase (3.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present disclosure relates to mutant thermostable glycosyl hydrolases family 7 enzymes, including mutant Trichoderma reesei endoglucanase I. In particular, the present disclosure relates to mutant thermostable enzymes, compositions containing the enzymes, and methods of use thereof.
PCT/US2011/047361 2010-09-15 2011-08-11 Thermophilic mutants of trichoderma reesei endoglucanase i Ceased WO2012036810A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2013529155A JP2013539971A (en) 2010-09-15 2011-08-11 Thermophilic mutants of endoglucanase I of TRICHODERMA REESEI
EP11825612.2A EP2616542A4 (en) 2010-09-15 2011-08-11 THERMOPHILIC MUTANTS OF ENDOGLUCANASE I FROM TRICHODERMA REESEI
CA2811206A CA2811206A1 (en) 2010-09-15 2011-08-11 Thermophilic mutants of trichoderma reesei endoglucanase i
BR112013006315A BR112013006315A2 (en) 2010-09-15 2011-08-11 thermophilic mutants of trichoderma reesei endoglycanase i
US13/822,623 US9410135B2 (en) 2010-09-15 2011-08-11 Thermophilic mutants of Trichoderma reesei endoglucanase I
CN2011800438167A CN103119159A (en) 2010-09-15 2011-08-11 Thermophilic mutants of trichoderma reesei endoglucanase I
AU2011302536A AU2011302536A1 (en) 2010-09-15 2011-08-11 Thermophilic mutants of Trichoderma reesei endoglucanase I

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US38324010P 2010-09-15 2010-09-15
US61/383,240 2010-09-15
US42208110P 2010-12-10 2010-12-10
US61/422,081 2010-12-10

Publications (3)

Publication Number Publication Date
WO2012036810A2 WO2012036810A2 (en) 2012-03-22
WO2012036810A3 WO2012036810A3 (en) 2012-07-05
WO2012036810A8 true WO2012036810A8 (en) 2013-05-30

Family

ID=45832157

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/047361 Ceased WO2012036810A2 (en) 2010-09-15 2011-08-11 Thermophilic mutants of trichoderma reesei endoglucanase i

Country Status (8)

Country Link
US (1) US9410135B2 (en)
EP (1) EP2616542A4 (en)
JP (1) JP2013539971A (en)
CN (1) CN103119159A (en)
AU (1) AU2011302536A1 (en)
BR (1) BR112013006315A2 (en)
CA (1) CA2811206A1 (en)
WO (1) WO2012036810A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2660319B1 (en) 2012-05-02 2016-09-14 Clariant Produkte (Deutschland) GmbH Endoglucanases with improved properties
AU2014283205A1 (en) * 2013-06-21 2015-12-17 Dupont Nutrition Biosciences Aps Method of preparing feed additive
EP3024930A2 (en) 2013-07-26 2016-06-01 Biomethodes Novel variant trichoderma reesei endoglucanases
EP3027743A1 (en) * 2013-07-29 2016-06-08 Danisco US Inc. Variant enzymes
PT3030661T (en) * 2013-08-08 2019-06-06 Danisco Us Inc Increasing the expression of a transgene in eukaryotic cells by reducing rna interference
FR3013731B1 (en) * 2013-11-22 2017-09-01 Ifp Energies Now ENDOGLUCANASIC VARIANTS WITH IMPROVED ACTIVITY AND USES THEREOF
FR3013732B1 (en) 2013-11-22 2016-10-21 Ifp Energies Now ENDOGLUCANASTIC VARIANTS WITH IMPROVED ACTIVITY AND USES THEREOF
CN104560922B (en) * 2014-03-25 2018-04-27 中国科学院青岛生物能源与过程研究所 A kind of cellulose restriction endonuclease Cel7B mutant of improved performance
CN104088185B (en) * 2014-06-11 2016-06-22 华南理工大学 A kind of biomass-based enzyme pretreatment method for bleaching of paper pulp
EP3728579A4 (en) * 2017-12-21 2021-12-01 AB Enzymes Oy VARIANTS OF MUSHROOM CELLULASE
MX2021011106A (en) * 2019-03-14 2021-10-22 Procter & Gamble Method for treating cotton.
CN109750015B (en) * 2019-03-27 2023-05-23 云南师范大学 A kind of xylanase mutant with improved thermostability and application thereof
CN109750016B (en) * 2019-03-27 2023-04-28 云南师范大学 Xylanase mutant with improved thermostability, preparation method and application thereof
US11987824B2 (en) * 2020-12-22 2024-05-21 Fornia Biosolutions, Inc. Additional endoglucanase variants and methods
CN114752583B (en) * 2022-03-30 2023-07-21 齐鲁工业大学 A heat-resistant β-1,3-1,4-glucanase mutant and its preparation method and application
CN116970591B (en) * 2023-09-19 2023-12-05 中国科学院天津工业生物技术研究所 Thermophilic endo-cellulase mutant and preparation method thereof
CN118480452B (en) * 2024-05-09 2024-12-31 湖北工业大学 Method for improving cellulase production of trichoderma reesei and application
CN120249080B (en) * 2025-06-09 2025-08-26 云南省农业科学院农业环境资源研究所 Trichoderma yunnanense and application thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0707640A4 (en) 1993-07-07 2000-10-11 Biomolecular Res Inst Ltd (1 $ m (7) 3, 1 $ m (7) 4) - $ 9 (b) -GLUCANOX WITH INCREASED STABILITY
US8637293B2 (en) * 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
DE60127661T2 (en) * 2000-04-13 2007-12-06 Mark Aaron Jupiter Emalfarb EXPRESSION REGULATING SEQUENCES OF MOLDED FUNGI CHRYSOSPORIUM
AU2003263842A1 (en) 2002-08-16 2004-03-03 Genencor International, Inc. Novel variant hyprocrea jecorina cbh1 cellulases
EP2298876A1 (en) 2003-03-21 2011-03-23 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
WO2005001065A2 (en) * 2003-04-01 2005-01-06 Genencor International, Inc. Variant humicola grisea cbh1.1
ATE447013T1 (en) * 2005-04-27 2009-11-15 Novozymes Inc POLYPEPTIDES WITH ENDOGLUCANASE ACTIVITY AND POLYNUCLEOTIDES CODING THEREFOR
MY160770A (en) 2006-02-10 2017-03-15 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
US8063267B2 (en) * 2006-03-30 2011-11-22 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2008148131A1 (en) * 2007-05-31 2008-12-04 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
CN102388134A (en) * 2009-01-28 2012-03-21 诺维信股份有限公司 Polypeptides having beta-glucosidase activity and polynucleotides encoding same
BR112012028206A2 (en) * 2010-06-01 2019-09-24 California Inst Of Techn stable, class I functional chimeric cellobiohydrolase enzymes.

Also Published As

Publication number Publication date
US9410135B2 (en) 2016-08-09
EP2616542A2 (en) 2013-07-24
US20150037845A1 (en) 2015-02-05
WO2012036810A3 (en) 2012-07-05
AU2011302536A1 (en) 2013-03-14
WO2012036810A2 (en) 2012-03-22
CN103119159A (en) 2013-05-22
JP2013539971A (en) 2013-10-31
CA2811206A1 (en) 2012-03-22
EP2616542A4 (en) 2014-01-22
BR112013006315A2 (en) 2017-07-04

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