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MX2012007184A - Acidos grasos de cadena ramificada y produccion biologica de estos. - Google Patents

Acidos grasos de cadena ramificada y produccion biologica de estos.

Info

Publication number
MX2012007184A
MX2012007184A MX2012007184A MX2012007184A MX2012007184A MX 2012007184 A MX2012007184 A MX 2012007184A MX 2012007184 A MX2012007184 A MX 2012007184A MX 2012007184 A MX2012007184 A MX 2012007184A MX 2012007184 A MX2012007184 A MX 2012007184A
Authority
MX
Mexico
Prior art keywords
conversion
fatty acid
cell
methylvalerate
oxobutanoate
Prior art date
Application number
MX2012007184A
Other languages
English (en)
Inventor
Charles Winston Saunders
Phillip Richard Green
Xu Jun
David Blair Cody
Zubin Sarosh Khambatta
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Publication of MX2012007184A publication Critical patent/MX2012007184A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/04—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with a disulfide as acceptor (1.2.4)
    • C12Y102/04004—3-Methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (1.2.4.4), i.e. branched-chain-alpha-ketoacid dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0118—Beta-ketoacyl-acyl-carrier-protein synthase III (2.3.1.180)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01182—(R)-Citramalate synthase (2.3.1.182)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y403/00—Carbon-nitrogen lyases (4.3)
    • C12Y403/01—Ammonia-lyases (4.3.1)
    • C12Y403/01019—Threonine ammonia-lyase (4.3.1.19)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se provee un método para producir ácido graso anteiso. El método comprende cultivar una célula que comprende al menos un polinucleótido exógeno o sobreexpresado que comprende una secuencia de ácido nucleico que codifica un polipéptido que cataliza al menos una de las siguientes reacciones: conversión de piruvato a citramalato; conversión de citramalato a citraconato; conversión de citraconato a ß-metil-D-malato; conversión de ß -metil-D-malato a 2-oxobutanoato; o conversión de treonina a 2-oxobutanoato, en condiciones que permiten la expresión del(os) polinucleótido(s) y la producción de ácido graso anteiso. Opcionalmente, la célula comprende, además, al menos un polinucleótido exógeno o sobreexpresado que comprende una secuencia de ácido nucleico que codifica un polipéptido que cataliza la conversión de 2-oxobutanoato a 2-aceto-2-hidroxi-butir ato, la conversión de 2-aceto-2- hidroxi-butirato a 2,3-dihidroxi-3-metilvalerato y/o la conversión de 2,3-dihidroxi-3- metilvalerato a a-ceto-3-metilvalerato. Además, se provee una célula que produce ácido graso anteiso y un método para usar la célula para producir ácido graso anteiso.
MX2012007184A 2009-12-22 2010-12-21 Acidos grasos de cadena ramificada y produccion biologica de estos. MX2012007184A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28903909P 2009-12-22 2009-12-22
US12/972,822 US20110151526A1 (en) 2009-12-22 2010-12-20 Branched-Chain Fatty Acids And Biological Production Thereof
PCT/US2010/061544 WO2011087787A1 (en) 2009-12-22 2010-12-21 Branched-chain fatty acids and biological production thereof

Publications (1)

Publication Number Publication Date
MX2012007184A true MX2012007184A (es) 2012-07-10

Family

ID=44151653

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012007184A MX2012007184A (es) 2009-12-22 2010-12-21 Acidos grasos de cadena ramificada y produccion biologica de estos.

Country Status (11)

Country Link
US (1) US20110151526A1 (es)
EP (1) EP2516650B1 (es)
CN (1) CN103097538B (es)
AU (1) AU2010341495A1 (es)
BR (1) BR112012015119A2 (es)
CA (1) CA2781730A1 (es)
ES (1) ES2525150T3 (es)
MX (1) MX2012007184A (es)
PH (1) PH12012501283A1 (es)
WO (1) WO2011087787A1 (es)
ZA (1) ZA201203767B (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
ES2693753T3 (es) * 2011-01-14 2018-12-13 REG Life Sciences, LLC Producción de ácidos grasos de cadena ramificada y derivados de los mismos en células microbianas recombinantes
MX366939B (es) * 2012-04-02 2019-07-31 Reg Life Sciences Llc Producción mejorada de derivados de ácidos grasos.
WO2014144461A2 (en) * 2013-03-15 2014-09-18 Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet) Engineered organisms for production of novel lipids
KR101747542B1 (ko) * 2015-10-23 2017-06-15 씨제이제일제당 (주) L-이소루신 생산능을 가지는 코리네박테리움 속 미생물 및 이를 이용하여 l-이소루신을 생산하는 방법
WO2017165397A1 (en) 2016-03-22 2017-09-28 University Of Georgia Research Foundation, Inc. Genetically engineered microbes and methods for producing citramalate
CN109722405B (zh) * 2017-10-31 2023-06-23 创享(天津)生物科技发展有限公司 利用葡萄糖生产异丙基吡喃酮的重组大肠杆菌及用途
EP3861122A1 (en) * 2018-10-04 2021-08-11 Anna University Production of l-2-aminobutyrate from citramalate,citraconate or 2-oxobutanoate
CN110982742B (zh) * 2019-12-12 2021-10-22 石河子大学 一种耐盐促生菌wp-3及其应用
CN116286569A (zh) * 2022-10-20 2023-06-23 江南大学 一种重组地衣芽孢杆菌全细胞转化产(r)-柠苹酸的方法
CN118638831B (zh) * 2024-08-13 2024-10-29 中国农业科学院北京畜牧兽医研究所 具有噬菌体抗性生产甲基苹果酸的大肠杆菌工程菌株、构建及发酵生产方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1044331A1 (zh) * 1999-06-25 2002-10-18 考格尼斯德国有限责任公司 基本上不饱和的支链脂肪醇
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
EP2194119A3 (en) 2006-05-19 2010-09-22 LS9, Inc. Production of fatty acids and derivatives thereof
SG182157A1 (en) 2007-06-01 2012-07-30 Solazyme Inc Production of oil in microorganisms
BRPI0920010A2 (pt) 2008-10-28 2016-04-19 Ls9 Inc métodos para produzir álcool graxo e surfactante
WO2010135624A2 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols

Also Published As

Publication number Publication date
US20110151526A1 (en) 2011-06-23
CN103097538B (zh) 2015-06-03
AU2010341495A1 (en) 2012-07-12
CN103097538A (zh) 2013-05-08
WO2011087787A1 (en) 2011-07-21
PH12012501283A1 (en) 2013-02-04
EP2516650B1 (en) 2014-09-17
ES2525150T3 (es) 2014-12-18
CA2781730A1 (en) 2011-07-21
EP2516650A1 (en) 2012-10-31
BR112012015119A2 (pt) 2017-03-28
ZA201203767B (en) 2014-10-29

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