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MX2012002532A - Metodos para mejorar la produccion de acido malico en hongos filamentosos. - Google Patents

Metodos para mejorar la produccion de acido malico en hongos filamentosos.

Info

Publication number
MX2012002532A
MX2012002532A MX2012002532A MX2012002532A MX2012002532A MX 2012002532 A MX2012002532 A MX 2012002532A MX 2012002532 A MX2012002532 A MX 2012002532A MX 2012002532 A MX2012002532 A MX 2012002532A MX 2012002532 A MX2012002532 A MX 2012002532A
Authority
MX
Mexico
Prior art keywords
dicarboxylic acid
methods
heterologous
filamentous fungal
fungal host
Prior art date
Application number
MX2012002532A
Other languages
English (en)
Inventor
Stephen Brown
Debbie Yaver
Sheryl Luttringer
Alan Berry
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Publication of MX2012002532A publication Critical patent/MX2012002532A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se relaciona con métodos para producir un ácido dicarboxílico de 4 átomos de carbono que comprende: (a) cultivar una célula hospedadora de hongo filamentoso que comprende un polinucleótido que se selecciona del grupo que consiste de un primer polinucleótido heterólogo que codifica para un transportador de ácido dicarboxílico de 4 átomos de carbono, un segundo polinucleótido heterólogo que codifica para una malato deshidrogenasa y un tercer polinucleótido heterólogo que codifica para una piruvato carboxilasa; en donde la célula hospedadora de hongo filamentoso es capaz de secretar concentraciones aumentadas del ácido dicarboxílico de 4 átomos de carbono en comparación con la célula hospedadora de hongo filamentoso sin el polinucleótido heterólogo cuando se cultiva bajo las mismas condiciones; y (b) recuperar el ácido dicarboxílico de 4 átomos de carbono. La presente invención también se relaciona con métodos para incrementar la producción de ácido dicarboxílico de 4 átomos de carbono, células hospedadoras de hongos filamentosos y variantes de malato deshidrogenasa.
MX2012002532A 2009-09-01 2010-08-27 Metodos para mejorar la produccion de acido malico en hongos filamentosos. MX2012002532A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US23896209P 2009-09-01 2009-09-01
US32722410P 2010-04-23 2010-04-23
US35697110P 2010-06-21 2010-06-21
PCT/US2010/047002 WO2011028643A1 (en) 2009-09-01 2010-08-27 Methods for improving malic acid production in filamentous fungi

Publications (1)

Publication Number Publication Date
MX2012002532A true MX2012002532A (es) 2012-04-11

Family

ID=43017152

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012002532A MX2012002532A (es) 2009-09-01 2010-08-27 Metodos para mejorar la produccion de acido malico en hongos filamentosos.

Country Status (10)

Country Link
US (3) US8741611B2 (es)
EP (2) EP3103876A1 (es)
JP (1) JP5833553B2 (es)
CN (1) CN102597224B (es)
AU (1) AU2010289725A1 (es)
BR (1) BR112012004594A2 (es)
CA (1) CA2772695A1 (es)
DK (1) DK2473600T3 (es)
MX (1) MX2012002532A (es)
WO (1) WO2011028643A1 (es)

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US10781462B2 (en) 2015-02-15 2020-09-22 Tianjin Institute Of Industrial Biotechnology, Chinese Academy Of Sciences Dibasic organic acid producing strain and preparation and application of same
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CN106778062B (zh) * 2016-11-29 2019-04-02 西南大学 出芽短梗霉基因组尺度代谢网络模型及其应用
BR112020012875A2 (pt) 2017-12-27 2021-01-05 St. Jude Children¿S Research Hospital, Inc. Moduladores de pequenas moléculas das pantotenato quinases
CN111787939B (zh) 2017-12-27 2024-09-13 圣朱德儿童研究医院有限公司 治疗castor相关疾病的方法
CN115819529B (zh) * 2018-10-19 2025-03-07 中国科学院天津工业生物技术研究所 提高苹果酸产量的新突变蛋白
CN109628472A (zh) * 2018-11-23 2019-04-16 山东理工大学 一种用于提高卷枝毛霉产油量的二羧酸转运体
CN109628473A (zh) * 2018-11-23 2019-04-16 山东理工大学 一种用于提高卷枝毛霉产油量的碳四二羧酸转运体
US20220282265A1 (en) * 2019-08-12 2022-09-08 Zymergen Inc. Engineered biosynthetic pathways for production of 3-amino-4-hydroxybenzoic acid by fermentation
JP7656889B2 (ja) 2019-11-14 2025-04-04 兵庫県公立大学法人 新規リンゴ酸脱水素酵素
CN110734865B (zh) * 2019-12-02 2022-11-01 南京昊禾生物科技有限公司 一种低pH条件下高产苹果酸的黑曲霉基因工程菌株及应用
CN111269950B (zh) * 2020-02-26 2022-03-08 安徽丰原发酵技术工程研究有限公司 发酵生产l-苹果酸的培养基及其制备方法与应用
CN111676146B (zh) * 2020-07-03 2022-02-01 江南大学 一株耐酸酿酒酵母及其应用
EP4262814A4 (en) * 2020-12-16 2025-02-26 St. Jude Children's Research Hospital, Inc. Methods of treating disorders associated with castor
EP4438714A1 (en) * 2021-11-24 2024-10-02 Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences Acid-resistant yeast strain for high-yield production of l-malic acid, and construction method therefor and use thereof
CN114806902B (zh) * 2022-05-12 2024-04-02 王玮 一种获取非转基因的里氏木霉定向基因工程改良株的方法和应用

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Also Published As

Publication number Publication date
CN102597224B (zh) 2015-07-15
DK2473600T3 (en) 2016-10-03
US20110053233A1 (en) 2011-03-03
EP2473600B1 (en) 2016-06-29
CN102597224A (zh) 2012-07-18
EP3103876A1 (en) 2016-12-14
JP5833553B2 (ja) 2015-12-16
US20150024446A1 (en) 2015-01-22
EP2473600A1 (en) 2012-07-11
WO2011028643A1 (en) 2011-03-10
US20140234925A1 (en) 2014-08-21
AU2010289725A1 (en) 2012-04-05
BR112012004594A2 (pt) 2016-06-21
CA2772695A1 (en) 2011-03-10
US8999685B2 (en) 2015-04-07
US8741611B2 (en) 2014-06-03
JP2013503631A (ja) 2013-02-04

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