[go: up one dir, main page]

MX2012000297A - Cofactores, donadores y aceptadores de ligasa quimicamente modificados. - Google Patents

Cofactores, donadores y aceptadores de ligasa quimicamente modificados.

Info

Publication number
MX2012000297A
MX2012000297A MX2012000297A MX2012000297A MX2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A MX 2012000297 A MX2012000297 A MX 2012000297A
Authority
MX
Mexico
Prior art keywords
modified
ligation
acceptors
donors
chemically modified
Prior art date
Application number
MX2012000297A
Other languages
English (en)
Inventor
Natasha Paul
Jonathan Shum
Alexandre Lebedev
Gerald Zon
Original Assignee
Trilink Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trilink Biotechnologies filed Critical Trilink Biotechnologies
Publication of MX2012000297A publication Critical patent/MX2012000297A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

En este documento se proporcionan métodos para la ligación de polinucleótidos que contienen componentes modificados de ligación, particularmente cofactores modificados, aceptores modificados y donadores modificados de ligasa. Los métodos se aplican fácilmente a los ensayos basados en ligación para la detección de una secuencia de ácido nucleico donde el uso del cofactor modificado mejora la discriminación entre moldes coincidentes y sin coincidencia. Además, el uso de los componentes modificados de ligación reduce o elimina la ligación en ausencia del molde de ácido nucleico.
MX2012000297A 2009-07-06 2010-07-06 Cofactores, donadores y aceptadores de ligasa quimicamente modificados. MX2012000297A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22336409P 2009-07-06 2009-07-06
PCT/US2010/041069 WO2011005762A1 (en) 2009-07-06 2010-07-06 Chemically modified ligase cofactors, donors and acceptors

Publications (1)

Publication Number Publication Date
MX2012000297A true MX2012000297A (es) 2012-04-30

Family

ID=43427762

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012000297A MX2012000297A (es) 2009-07-06 2010-07-06 Cofactores, donadores y aceptadores de ligasa quimicamente modificados.

Country Status (9)

Country Link
US (1) US8728725B2 (es)
EP (1) EP2451980B1 (es)
JP (1) JP5903379B2 (es)
AU (1) AU2010270715C1 (es)
CA (1) CA2767408C (es)
ES (1) ES2521740T3 (es)
MX (1) MX2012000297A (es)
NZ (1) NZ597535A (es)
WO (1) WO2011005762A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3211126T3 (da) 2009-02-13 2020-12-21 X Chem Inc Fremgangsmåder til dannelse og screening af dna-kodede biblioteker
US9631227B2 (en) 2009-07-06 2017-04-25 Trilink Biotechnologies, Inc. Chemically modified ligase cofactors, donors and acceptors
ES2632768T3 (es) * 2011-01-17 2017-09-15 Life Technologies Corporation Flujo de trabajo para la detección de ligandos empleando ácidos nucleicos
EP3263703B1 (en) 2011-01-17 2020-04-22 Life Technologies Corporation Enzymatic ligation of nucleic acids
EP3351631B1 (en) 2011-09-07 2020-11-04 X-Chem, Inc. Methods for tagging dna-encoded libraries
US9403863B2 (en) 2011-09-12 2016-08-02 Idenix Pharmaceuticals Llc Substituted carbonyloxymethylphosphoramidate compounds and pharmaceutical compositions for the treatment of viral infections
WO2013177195A1 (en) 2012-05-22 2013-11-28 Idenix Pharmaceuticals, Inc. 3',5'-cyclic phosphate prodrugs for hcv infection
MX355708B (es) 2012-05-22 2018-04-27 Idenix Pharmaceuticals Llc Compuestos de d-aminoacidos para enfermedades del higado.
WO2013177188A1 (en) 2012-05-22 2013-11-28 Idenix Pharmaceuticals, Inc. 3',5'-cyclic phosphoramidate prodrugs for hcv infection
TW201408688A (zh) 2012-05-25 2014-03-01 Janssen R & D Ireland 尿嘧啶基螺氧環丁烷（ｓｐｉｒｏｏｘｅｔａｎｅ）核苷類
EP2900682A1 (en) 2012-09-27 2015-08-05 IDENIX Pharmaceuticals, Inc. Esters and malonates of sate prodrugs
AP2015008384A0 (en) 2012-10-08 2015-04-30 Univ Montpellier Ct Nat De La Rech Scient 2'-Chloro nucleoside analogs for hcv infection
US9211300B2 (en) 2012-12-19 2015-12-15 Idenix Pharmaceuticals Llc 4′-fluoro nucleosides for the treatment of HCV
WO2014137926A1 (en) 2013-03-04 2014-09-12 Idenix Pharmaceuticals, Inc. 3'-deoxy nucleosides for the treatment of hcv
US9339541B2 (en) 2013-03-04 2016-05-17 Merck Sharp & Dohme Corp. Thiophosphate nucleosides for the treatment of HCV
US20140271547A1 (en) 2013-03-13 2014-09-18 Idenix Pharmaceuticals, Inc. Amino acid phosphoramidate pronucleotides of 2'-cyano, azido and amino nucleosides for the treatment of hcv
WO2014165542A1 (en) 2013-04-01 2014-10-09 Idenix Pharmaceuticals, Inc. 2',4'-fluoro nucleosides for the treatment of hcv
WO2014197578A1 (en) 2013-06-05 2014-12-11 Idenix Pharmaceuticals, Inc. 1',4'-thio nucleosides for the treatment of hcv
US20150037282A1 (en) 2013-08-01 2015-02-05 Idenix Pharmaceuticals, Inc. D-amino acid phosphoramidate pronucleotides of halogeno pyrimidine compounds for liver disease
WO2015161137A1 (en) * 2014-04-16 2015-10-22 Idenix Pharmaceuticals, Inc. 3'-substituted methyl or alkynyl nucleosides for the treatment of hcv
CN106591425A (zh) * 2015-10-15 2017-04-26 北京寻因生物科技有限公司 基于连接反应的多重靶向检测核酸指标的方法
CN106916877A (zh) * 2015-12-24 2017-07-04 广州好芝生物科技有限公司 一种结核分枝杆菌利福平耐药突变检测试剂盒
JP7401902B2 (ja) 2017-05-08 2023-12-20 コデクシス， インコーポレイテッド 遺伝子操作されたリガーゼバリアント
CN108179180A (zh) * 2017-10-25 2018-06-19 广州和康医疗技术有限公司 一种对cyp3a5*3位点进行基因型检测的方法和试剂盒
CN117561269A (zh) * 2021-07-08 2024-02-13 深圳华大生命科学研究院 修饰的核苷或核苷酸

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
US5508179A (en) 1994-03-18 1996-04-16 Bio-Rad Laboratories, Inc. Use of deoxyribose nicotinamide adenine dinucleotide to enhance the specificity of NAD+ -dependent ligation reactions
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
EP1163251A1 (en) 1999-03-24 2001-12-19 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES N-acylphosphoramidites and their use in oligonucleotide synthesis
US6297016B1 (en) 1999-10-08 2001-10-02 Applera Corporation Template-dependent ligation with PNA-DNA chimeric probes
US6635425B2 (en) 2001-01-05 2003-10-21 Molecular Staging, Inc. 5′-thio phosphate directed ligation of oligonucleotides and use in detection of single nucleotide polymorphisms
JP5117722B2 (ja) * 2003-09-02 2013-01-16 キージーン ナムローゼ フェンノートシャップ 標的核酸配列の検出のためのｏｌａベースの方法
WO2012094343A1 (en) 2011-01-05 2012-07-12 Trilink Biotechnologies Chemically substituted thermosensitive probes and cofactors for hot start ligation

Also Published As

Publication number Publication date
EP2451980A1 (en) 2012-05-16
US8728725B2 (en) 2014-05-20
CA2767408C (en) 2018-01-23
ES2521740T3 (es) 2014-11-13
JP5903379B2 (ja) 2016-04-13
AU2010270715C1 (en) 2016-03-17
NZ597535A (en) 2013-12-20
AU2010270715B2 (en) 2015-06-25
EP2451980A4 (en) 2012-10-31
WO2011005762A1 (en) 2011-01-13
US20110008788A1 (en) 2011-01-13
AU2010270715A1 (en) 2012-02-02
CA2767408A1 (en) 2011-01-13
EP2451980B1 (en) 2014-05-21
JP2012531928A (ja) 2012-12-13

Similar Documents

Publication Publication Date Title
MX2012000297A (es) Cofactores, donadores y aceptadores de ligasa quimicamente modificados.
HK1220234A1 (zh) 化学修饰的连接酶辅因子、供体和受体
EP2369454A4 (en) MANUSCRIPTED INPUT / OUTPUT SYSTEM, MANUSCRIPTED INPUT SHEET, INFORMATION INPUT SYSTEM, AND INFORMATION INPUT HELP SHEET
EP2339576A3 (en) Multi-modal input on an electronic device
WO2011100228A3 (en) Identification of message recipients
WO2009124197A3 (en) Quick record function in a smart pen computing system
EP4276848A3 (en) Methods and articles of manufacture for hosting a safety critical application on an uncontrolled data processing device
WO2009049916A3 (en) Method for determining methylation at cytosine residues
WO2011044298A3 (en) Systems and methods for establishing an environmental representation
WO2008088652A3 (en) Method and system for generating a predictive analysis of the performance of peer reviews
IN2012DN06299A (es)
WO2007127166A3 (en) Systems and methods for generating media content using microtrends
WO2008008919A3 (en) Methods and systems for sequence-based design of multiple reaction monitoring transitions and experiments
NZ705075A (en) Method and system for selectively biased linear discriminant analysis in automatic speech recognition systems
WO2013059293A9 (en) Single, direct detection of hemoglobin a1c percentage using enzyme triggered redox altering chemical elimination (e-trace) immunoassay
AU2009355009A8 (en) THD primer target detection
WO2010017486A3 (en) Analyzing a content-requesting media item
WO2010014527A3 (en) Building a research document based on implicit/explicit actions
TW200943180A (en) Computer system
WO2008122088A8 (en) Methods for detecting a target nucleotide sequence in a sample utilising a nuclease-aptamer complex
WO2012159060A3 (en) Methods, systems, and compositions for detection of microbial dna by pcr
WO2008094680A3 (en) Financial instrument based on content and methods for valuation
TW200943175A (en) Synchronization control method and information processing device
WO2010004562A3 (en) Methods and compositions for detecting colorectal cancer
WO2010093820A3 (en) Combinations of polymorphisms for determining allele-specific expression of igf2

Legal Events

Date Code Title Description
FG Grant or registration