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MX2012000087A - Metodo cromatografico basado en gradiente no lineal preparativo y productos purificados del mismo. - Google Patents

Metodo cromatografico basado en gradiente no lineal preparativo y productos purificados del mismo.

Info

Publication number
MX2012000087A
MX2012000087A MX2012000087A MX2012000087A MX2012000087A MX 2012000087 A MX2012000087 A MX 2012000087A MX 2012000087 A MX2012000087 A MX 2012000087A MX 2012000087 A MX2012000087 A MX 2012000087A MX 2012000087 A MX2012000087 A MX 2012000087A
Authority
MX
Mexico
Prior art keywords
linear gradient
chromatographic method
purified products
gradient based
based chromatographic
Prior art date
Application number
MX2012000087A
Other languages
English (en)
Inventor
Nitesh Dave
Sundaresh Shankar
Devesh Radhakrishnan
Harish Iyer
Krishanachaitanya Gulla
Original Assignee
Biocon Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocon Ltd filed Critical Biocon Ltd
Publication of MX2012000087A publication Critical patent/MX2012000087A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
    • B01D15/203—Equilibration or regeneration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente descripción proporciona un método para purificación de péptidos por técnicas cromatográficas. La metodología propuesta ayudará a resolver los problemas asociados en purificación de productos de proteína biológica que surgen de la industria de biotecnología en evolución.
MX2012000087A 2009-07-09 2010-07-08 Metodo cromatografico basado en gradiente no lineal preparativo y productos purificados del mismo. MX2012000087A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN1639CH2009 2009-07-09
PCT/IN2010/000459 WO2011021210A1 (en) 2009-07-09 2010-07-08 A preparative non-linear gradient based chromatographic method and purified products thereof

Publications (1)

Publication Number Publication Date
MX2012000087A true MX2012000087A (es) 2012-02-28

Family

ID=43606703

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012000087A MX2012000087A (es) 2009-07-09 2010-07-08 Metodo cromatografico basado en gradiente no lineal preparativo y productos purificados del mismo.

Country Status (13)

Country Link
US (1) US20120123089A1 (es)
EP (1) EP2451824A4 (es)
JP (1) JP2012532862A (es)
KR (1) KR20120044358A (es)
CN (1) CN102471367A (es)
AU (1) AU2010286059A1 (es)
BR (1) BRPI1016071A2 (es)
CA (1) CA2766571A1 (es)
IL (1) IL217323A0 (es)
MX (1) MX2012000087A (es)
RU (1) RU2489441C1 (es)
SG (1) SG176985A1 (es)
WO (1) WO2011021210A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016172855A1 (zh) * 2015-04-28 2016-11-03 深圳翰宇药业股份有限公司 多肽混合物高效液相色谱分析方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK353781A (da) * 1981-08-10 1983-02-11 Novo Industri As Fremgangsmaade til fremstilling af insulinderivater
US5514646A (en) * 1989-02-09 1996-05-07 Chance; Ronald E. Insulin analogs modified at position 29 of the B chain
DE4028120C2 (de) * 1990-09-05 1996-09-19 Hoechst Ag Verfahren zur Reinigung von Insulin und/oder Insulinderivaten
DE19726167B4 (de) * 1997-06-20 2008-01-24 Sanofi-Aventis Deutschland Gmbh Insulin, Verfahren zu seiner Herstellung und es enthaltende pharmazeutische Zubereitung
DE19838097A1 (de) * 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Verfahren zur chromatographischen Reinigung von Insulinen
US6451987B1 (en) * 1999-03-15 2002-09-17 Novo Nordisk A/S Ion exchange chromatography of proteins and peptides
ES2361166T3 (es) * 2005-07-08 2011-06-14 Biocon Limited Conjugados de insulina.
CN101291952B (zh) * 2005-10-13 2016-02-03 拜奥康有限公司 制备胰岛素缀合物的方法
MX2009013421A (es) * 2007-06-13 2010-03-22 Sterrenbeld Biotechnologie North America Inc Mamiferos transgenicos que producen proteinas exogenas en su leche.
BRPI0822775A2 (pt) * 2008-02-19 2015-06-30 Biocon Ltd Método de obtenção de uma proteína heteróloga, purificada, biologicamente ativa.
WO2011018745A1 (en) * 2009-08-11 2011-02-17 Biocon Limited Chromatographic processes and purified compounds thereof

Also Published As

Publication number Publication date
IL217323A0 (en) 2012-02-29
WO2011021210A1 (en) 2011-02-24
US20120123089A1 (en) 2012-05-17
BRPI1016071A2 (pt) 2019-09-24
CN102471367A (zh) 2012-05-23
AU2010286059A1 (en) 2012-02-02
EP2451824A1 (en) 2012-05-16
SG176985A1 (en) 2012-01-30
CA2766571A1 (en) 2011-02-24
JP2012532862A (ja) 2012-12-20
EP2451824A4 (en) 2013-04-24
RU2489441C1 (ru) 2013-08-10
KR20120044358A (ko) 2012-05-07

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