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MX2010008920A - Estabilizacion de deshidrogenasas utilizando coenzimas estables. - Google Patents

Estabilizacion de deshidrogenasas utilizando coenzimas estables.

Info

Publication number
MX2010008920A
MX2010008920A MX2010008920A MX2010008920A MX2010008920A MX 2010008920 A MX2010008920 A MX 2010008920A MX 2010008920 A MX2010008920 A MX 2010008920A MX 2010008920 A MX2010008920 A MX 2010008920A MX 2010008920 A MX2010008920 A MX 2010008920A
Authority
MX
Mexico
Prior art keywords
dehydrogenases
stabilization
enzyme
stable
stable coenzymes
Prior art date
Application number
MX2010008920A
Other languages
English (en)
Inventor
Joachim Hoenes
Dieter Heindl
Carina Horn
Claudia Gaessler-Dietsche
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2010008920A publication Critical patent/MX2010008920A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01028—D-Lactate dehydrogenase (1.1.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01047—Glucose 1-dehydrogenase (1.1.1.47)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01005—L-Amino-acid dehydrogenase (1.4.1.5)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a un método para estabilizar una enzima mediante el almacenamiento de la enzima en la presencia de una coenzima estable. Además, la presente invención se refiere a una enzima estabilizada utilizando una coenzima estable, y al uso del mismo en elementos de prueba para detectar analitos.
MX2010008920A 2008-02-19 2009-02-19 Estabilizacion de deshidrogenasas utilizando coenzimas estables. MX2010008920A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08003054A EP2093284A1 (de) 2008-02-19 2008-02-19 Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
PCT/EP2009/001206 WO2009103540A1 (de) 2008-02-19 2009-02-19 Stabilisierung von dehydrogenasen mit stabilen coenzymen

Publications (1)

Publication Number Publication Date
MX2010008920A true MX2010008920A (es) 2010-09-07

Family

ID=39768713

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010008920A MX2010008920A (es) 2008-02-19 2009-02-19 Estabilizacion de deshidrogenasas utilizando coenzimas estables.

Country Status (15)

Country Link
US (2) US9896666B2 (es)
EP (2) EP2093284A1 (es)
JP (2) JP5758128B2 (es)
KR (3) KR101381004B1 (es)
CN (3) CN104450659A (es)
AR (1) AR070436A1 (es)
AU (1) AU2009216908B9 (es)
BR (1) BRPI0907839A2 (es)
CA (1) CA2721718C (es)
HK (1) HK1208703A1 (es)
MX (1) MX2010008920A (es)
RU (1) RU2499834C2 (es)
TW (1) TWI482860B (es)
WO (1) WO2009103540A1 (es)
ZA (1) ZA201006638B (es)

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EP2226008A1 (de) 2009-02-19 2010-09-08 Roche Diagnostics GmbH Verfahren zur Herstellung eines analytischen Magazins
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
KR101604624B1 (ko) * 2009-02-19 2016-03-28 에프. 호프만-라 로슈 아게 건식 화학 층에서 낮은 활성을 갖는 효소의 고속 반응 속도
EP2292751A1 (de) * 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
CA2782943C (en) * 2009-12-16 2014-09-09 F. Hoffmann-La Roche Ag Detecting the decomposition of enzymes in a test element by means of controlled release of a protected analyte
EP2636750A1 (en) * 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
CN107024474B (zh) 2012-06-22 2020-11-06 霍夫曼-拉罗奇有限公司 用于检测体液中的分析物的方法和器件
US8920628B2 (en) 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Systems and methods for multiple analyte analysis
US8921061B2 (en) * 2012-11-02 2014-12-30 Roche Diagnostics Operations, Inc. Reagent materials and associated test elements
PL2936124T3 (pl) 2012-12-20 2017-08-31 F.Hoffmann-La Roche Ag Sposoby oceniania medycznych krzywych pomiarowych
WO2014096184A1 (en) 2012-12-20 2014-06-26 Roche Diagnostics Gmbh Method for analyzing a sample of a body fluid
EP2781919A1 (en) 2013-03-19 2014-09-24 Roche Diagniostics GmbH Method / device for generating a corrected value of an analyte concentration in a sample of a body fluid
EP2994536B1 (en) 2013-05-08 2017-06-14 Roche Diabetes Care GmbH Stabilization of enzymes by nicotinic acid
CN105247069A (zh) * 2013-06-04 2016-01-13 豪夫迈·罗氏有限公司 用于fret的新化合物及与其相关的方法
EP3063169B1 (en) 2013-10-29 2018-10-10 F. Hoffmann-La Roche AG Nano-enzyme containers for test elements
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EP2927319A1 (en) 2014-03-31 2015-10-07 Roche Diagnostics GmbH High load enzyme immobilization by crosslinking
CN106164054B (zh) 2014-04-14 2019-05-07 豪夫迈·罗氏有限公司 吩嗪介导剂
CN106573910B (zh) 2014-08-22 2020-08-28 豪夫迈·罗氏有限公司 氧化还原指示剂
CN107779459B (zh) * 2016-08-31 2021-06-08 安琪酵母股份有限公司 葡萄糖脱氢酶dna分子、载体和菌株及应用
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EP3339431A1 (en) 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
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Also Published As

Publication number Publication date
BRPI0907839A2 (pt) 2015-08-11
CN101945999B (zh) 2016-06-08
EP2245152A1 (de) 2010-11-03
AU2009216908B2 (en) 2012-04-19
ZA201006638B (en) 2011-06-29
HK1208703A1 (en) 2016-03-11
US20180265849A1 (en) 2018-09-20
EP2093284A1 (de) 2009-08-26
JP5758128B2 (ja) 2015-08-05
JP2012517816A (ja) 2012-08-09
KR20100118995A (ko) 2010-11-08
RU2499834C2 (ru) 2013-11-27
US20110143416A1 (en) 2011-06-16
CN104450659A (zh) 2015-03-25
WO2009103540A1 (de) 2009-08-27
US9896666B2 (en) 2018-02-20
KR20120134142A (ko) 2012-12-11
TW200951222A (en) 2009-12-16
CN102325896A (zh) 2012-01-18
EP2245152B1 (de) 2019-05-01
AR070436A1 (es) 2010-04-07
AU2009216908A1 (en) 2009-08-27
CN101945999A (zh) 2011-01-12
JP2011514153A (ja) 2011-05-06
JP6113405B2 (ja) 2017-04-12
KR101381004B1 (ko) 2014-04-08
US11220674B2 (en) 2022-01-11
KR20130081300A (ko) 2013-07-16
CA2721718A1 (en) 2009-08-27
TWI482860B (zh) 2015-05-01
AU2009216908B9 (en) 2012-07-05
RU2010138610A (ru) 2012-03-27
CA2721718C (en) 2017-11-28

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