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MX2009010840A - Metodo de seleccion para internalizar acidos nucleicos. - Google Patents

Metodo de seleccion para internalizar acidos nucleicos.

Info

Publication number
MX2009010840A
MX2009010840A MX2009010840A MX2009010840A MX2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A MX 2009010840 A MX2009010840 A MX 2009010840A
Authority
MX
Mexico
Prior art keywords
nucleic acids
selection method
cell internalizing
cells
internalizing nucleic
Prior art date
Application number
MX2009010840A
Other languages
English (en)
Inventor
Andrew D Ellington
Matthew Levy
Amy Yan
Chi-Tai Chu
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of MX2009010840A publication Critical patent/MX2009010840A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención incluye composiciones y métodos para poner en contacto una o más células con una genoteca aleatoria que contiene ARN, tratar las células puestas en contacto con un agente desnaturalizante o digestión con una o más nucleasas, y extraer de las células uno o más ácidos nucleicos internalizados resistentes a las nucleasas o desnaturalizantes.
MX2009010840A 2007-04-09 2008-04-09 Metodo de seleccion para internalizar acidos nucleicos. MX2009010840A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US91079207P 2007-04-09 2007-04-09
US98465607P 2007-11-01 2007-11-01
PCT/US2008/059805 WO2008124798A1 (en) 2007-04-09 2008-04-09 Selection method for cell internalizing nucleic acids

Publications (1)

Publication Number Publication Date
MX2009010840A true MX2009010840A (es) 2009-11-05

Family

ID=39831424

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009010840A MX2009010840A (es) 2007-04-09 2008-04-09 Metodo de seleccion para internalizar acidos nucleicos.

Country Status (12)

Country Link
US (1) US8298764B2 (es)
EP (1) EP2140034B1 (es)
JP (1) JP5368425B2 (es)
KR (1) KR20100015558A (es)
CN (1) CN101680037B (es)
AU (1) AU2008237057A1 (es)
BR (1) BRPI0810828A2 (es)
CA (1) CA2683602A1 (es)
ES (1) ES2394384T3 (es)
IL (1) IL201315A (es)
MX (1) MX2009010840A (es)
WO (1) WO2008124798A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540412B2 (en) * 2010-05-13 2017-01-10 Albert Einstein College Of Medicine, Inc. Methods of preparing targeted aptamer prodrugs
US20130150251A1 (en) * 2011-10-28 2013-06-13 The Curators Of The University Of Missouri Methods for analyzing lariat rna
KR101352430B1 (ko) * 2011-12-02 2014-01-20 건국대학교 산학협력단 Ｈａ１에 특이적으로 결합하는 ｒｎａ 앱타머 및 조류인플루엔자 바이러스에 대한 응용
US20130203057A1 (en) * 2012-01-20 2013-08-08 Biohelix Corporation Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions
US11078378B2 (en) 2015-03-31 2021-08-03 Asahi Kasei Kabushiki Kaisha Polyimide film, polyimide varnish, and product and layered product using the polyimide film
WO2017047750A1 (ja) * 2015-09-18 2017-03-23 公立大学法人大阪市立大学 細胞内移行性ｄｎａ及びそれを用いた目的分子の細胞内導入方法
JP7219473B2 (ja) * 2017-08-28 2023-02-08 静岡県公立大学法人 コリバクチンおよびコリバクチン産生菌の検出方法および検出プローブ

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7767803B2 (en) * 2002-06-18 2010-08-03 Archemix Corp. Stabilized aptamers to PSMA and their use as prostate cancer therapeutics
US20050256071A1 (en) * 2003-07-15 2005-11-17 California Institute Of Technology Inhibitor nucleic acids
US8097238B2 (en) * 2004-02-27 2012-01-17 Peter Maccallum Cancer Institute Cell targeting conjugates
US20060105975A1 (en) * 2004-04-19 2006-05-18 Shannon Pendergrast Aptamer-mediated intracellular delivery of therapeutic oligonucleotides
WO2006096754A2 (en) 2005-03-07 2006-09-14 Archemix Corp. Stabilized aptamers to psma and their use as prostate cancer therapeutics

Also Published As

Publication number Publication date
US8298764B2 (en) 2012-10-30
IL201315A (en) 2013-04-30
JP2010523157A (ja) 2010-07-15
EP2140034A1 (en) 2010-01-06
CN101680037A (zh) 2010-03-24
AU2008237057A1 (en) 2008-10-16
EP2140034A4 (en) 2011-04-06
BRPI0810828A2 (pt) 2014-10-29
CA2683602A1 (en) 2008-10-16
JP5368425B2 (ja) 2013-12-18
KR20100015558A (ko) 2010-02-12
IL201315A0 (en) 2010-05-31
US20090170711A1 (en) 2009-07-02
ES2394384T3 (es) 2013-01-31
WO2008124798A1 (en) 2008-10-16
CN101680037B (zh) 2012-12-05
EP2140034B1 (en) 2012-11-07

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