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MX2008000345A - Visualizacion en fagos usando transcolocacion contraduccional de polipeptidos de fusion. - Google Patents

Visualizacion en fagos usando transcolocacion contraduccional de polipeptidos de fusion.

Info

Publication number
MX2008000345A
MX2008000345A MX2008000345A MX2008000345A MX2008000345A MX 2008000345 A MX2008000345 A MX 2008000345A MX 2008000345 A MX2008000345 A MX 2008000345A MX 2008000345 A MX2008000345 A MX 2008000345A MX 2008000345 A MX2008000345 A MX 2008000345A
Authority
MX
Mexico
Prior art keywords
phage
polypeptides
phage display
particle
cotranslational translocation
Prior art date
Application number
MX2008000345A
Other languages
English (en)
Inventor
Daniel Steiner
Patrik Forrer
Michael T Stumpp
Andreas Pluckthun
Original Assignee
Univ Zuerich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Zuerich filed Critical Univ Zuerich
Publication of MX2008000345A publication Critical patent/MX2008000345A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)

Abstract

La presente invencion se refiere a un metodo de visualizacion en fagos filamentosos en done los polipeptidos de interes visualizados en la particula de fago se transcolocan de manera contraduccional a traves de la membrana citoplasmica de bacterias gram-negativas en base a la ruta de particulas de reconocimiento de senal. Este metodo es particularmente adecuado para la polipeptidos, que se conoce que son dificiles de visualizar en los fagos, y para proteinas de bibliotecas de ADNc y otras bibliotecas de combinacion, en particular cuando se derivan de nucleos de proteinas estables de plegado muy rapido. La invencion se refiere ademas a vectores de fagos o fagomidos utiles en el metodo que comprende una construccion genica que codifica para un polipeptido de fusion que comprende un polipeptido que se va a visualizar en la particula de fago y una secuencia de senal N-terminal que promueve la transcolocacion contraduccional.
MX2008000345A 2005-07-08 2006-06-30 Visualizacion en fagos usando transcolocacion contraduccional de polipeptidos de fusion. MX2008000345A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05106236 2005-07-08
PCT/EP2006/063729 WO2007006665A1 (en) 2005-07-08 2006-06-30 Phage display using cotranslational translocation of fusion polypeptides

Publications (1)

Publication Number Publication Date
MX2008000345A true MX2008000345A (es) 2008-04-02

Family

ID=35063135

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2008000345A MX2008000345A (es) 2005-07-08 2006-06-30 Visualizacion en fagos usando transcolocacion contraduccional de polipeptidos de fusion.

Country Status (20)

Country Link
US (2) US8846577B2 (es)
EP (1) EP1902131B1 (es)
JP (1) JP5042218B2 (es)
KR (1) KR101268939B1 (es)
CN (1) CN101213299B (es)
AT (1) ATE448305T1 (es)
AU (1) AU2006268780B2 (es)
BR (1) BRPI0613593A2 (es)
CA (1) CA2614512C (es)
DE (1) DE602006010386D1 (es)
DK (1) DK1902131T3 (es)
ES (1) ES2335136T3 (es)
IL (1) IL188518A (es)
MX (1) MX2008000345A (es)
NO (1) NO20080665L (es)
NZ (1) NZ564695A (es)
PL (1) PL1902131T3 (es)
RU (1) RU2412249C2 (es)
WO (1) WO2007006665A1 (es)
ZA (1) ZA200710687B (es)

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PL2468869T3 (pl) * 2007-01-31 2015-08-31 Pfenex Inc Bakteryjna sekwencja wiodąca dla zwiększonej ekspresji
BRPI0921469B1 (pt) 2008-11-03 2022-01-18 Molecular Partners Ag Proteínas de ligação que inibem a interação com o receptor de vegf-a, composição farmacêutica
GB0917647D0 (en) * 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
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US9284361B2 (en) 2010-11-26 2016-03-15 Molecular Partners Ag Designed repeat proteins binding to serum albumin
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WO2014031566A1 (en) 2012-08-22 2014-02-27 Immunogen, Inc. Cytotoxic benzodiazepine derivatives
EP2738180A1 (en) 2012-11-30 2014-06-04 Molecular Partners AG Binding proteins comprising at least two binding domains against HER2.
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US9901647B2 (en) 2013-02-28 2018-02-27 Immunogen, Inc. Conjugates comprising cell-binding agents and cytotoxic agents
CN105209483B (zh) 2013-05-31 2021-07-27 分子组合公司 与肝细胞生长因子结合的设计锚蛋白重复蛋白
WO2014194030A2 (en) 2013-05-31 2014-12-04 Immunogen, Inc. Conjugates comprising cell-binding agents and cytotoxic agents
MX2016011637A (es) * 2014-03-14 2017-04-13 Genentech Inc Metodos y composiciones para secrecion de polipeptidos heterologos.
MX2016012285A (es) 2014-03-24 2017-01-23 Genentech Inc Tratamiento de cáncer con antagonista de c-met y correlación de estos con la expresión de hgf.
US9381256B2 (en) 2014-09-03 2016-07-05 Immunogen, Inc. Cytotoxic benzodiazepine derivatives
KR102632830B1 (ko) 2014-09-03 2024-02-02 이뮤노젠 아이엔씨 세포독성 벤조다이아제핀 유도체
EP3277711B9 (en) 2015-04-02 2023-08-16 Molecular Partners AG Designed ankyrin repeat domains with binding specificity for serum albumin
US10220180B2 (en) 2015-10-16 2019-03-05 Milestone Scientific, Inc. Method and apparatus for performing a peripheral nerve block
CN109843310A (zh) * 2016-08-31 2019-06-04 哈佛学院院长等 分泌治疗性蛋白的工程化细菌及其使用方法
AU2017331329B2 (en) 2016-09-22 2020-10-29 Molecular Partners Ag Recombinant binding proteins and their use
US10632255B2 (en) 2017-02-15 2020-04-28 Milestone Scientific, Inc. Drug infusion device
IL313912A (en) 2017-02-28 2024-08-01 Immunogen Inc Maytansinoid derivatives with self-immolative peptide linkers and conjugates thereof
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Also Published As

Publication number Publication date
BRPI0613593A2 (pt) 2011-01-18
US20110118146A1 (en) 2011-05-19
CN101213299A (zh) 2008-07-02
CN101213299B (zh) 2013-03-27
JP2009500027A (ja) 2009-01-08
RU2008104174A (ru) 2009-08-20
ZA200710687B (en) 2008-11-26
WO2007006665A1 (en) 2007-01-18
ATE448305T1 (de) 2009-11-15
US9447142B2 (en) 2016-09-20
ES2335136T3 (es) 2010-03-22
JP5042218B2 (ja) 2012-10-03
IL188518A0 (en) 2008-04-13
DK1902131T3 (da) 2010-03-01
CA2614512A1 (en) 2007-01-18
CA2614512C (en) 2014-01-07
DE602006010386D1 (en) 2009-12-24
EP1902131A1 (en) 2008-03-26
HK1122839A1 (en) 2009-05-29
AU2006268780A1 (en) 2007-01-18
IL188518A (en) 2011-04-28
EP1902131B1 (en) 2009-11-11
US8846577B2 (en) 2014-09-30
PL1902131T3 (pl) 2010-05-31
KR20080035603A (ko) 2008-04-23
NO20080665L (no) 2008-02-05
AU2006268780B2 (en) 2011-03-24
NZ564695A (en) 2009-02-28
US20150057186A1 (en) 2015-02-26
RU2412249C2 (ru) 2011-02-20
KR101268939B1 (ko) 2013-05-30

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