ME01566B - POBOLJŠANI HUMANI DUGI PENTRAKSIN 3 EKSPESIONI SISTEM l NJEGOVE POTREBE - Google Patents
POBOLJŠANI HUMANI DUGI PENTRAKSIN 3 EKSPESIONI SISTEM l NJEGOVE POTREBEInfo
- Publication number
- ME01566B ME01566B MEP-2013-126A MEP12613A ME01566B ME 01566 B ME01566 B ME 01566B ME P12613 A MEP12613 A ME P12613A ME 01566 B ME01566 B ME 01566B
- Authority
- ME
- Montenegro
- Prior art keywords
- recombinant
- human
- long pentraxin
- clone
- recombinant human
- Prior art date
Links
- 101001082142 Homo sapiens Pentraxin-related protein PTX3 Proteins 0.000 title claims 4
- 102100027351 Pentraxin-related protein PTX3 Human genes 0.000 title claims 4
- 108700025763 PTX3 Proteins 0.000 claims 8
- 210000005260 human cell Anatomy 0.000 claims 8
- BFHAYPLBUQVNNJ-UHFFFAOYSA-N Pectenotoxin 3 Natural products OC1C(C)CCOC1(O)C1OC2C=CC(C)=CC(C)CC(C)(O3)CCC3C(O3)(O4)CCC3(C=O)CC4C(O3)C(=O)CC3(C)C(O)C(O3)CCC3(O3)CCCC3C(C)C(=O)OC2C1 BFHAYPLBUQVNNJ-UHFFFAOYSA-N 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 239000013598 vector Substances 0.000 claims 3
- 210000004027 cell Anatomy 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000013604 expression vector Substances 0.000 claims 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 210000004185 liver Anatomy 0.000 claims 1
- 239000003550 marker Substances 0.000 claims 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000001542 size-exclusion chromatography Methods 0.000 claims 1
- 238000001890 transfection Methods 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Peptides Or Proteins (AREA)
Claims (8)
1.Upotreba eukariotskogekspresionog vektora koji sadrži sekvencu nukleotida šifrovanu za humani dugi pentraksin PTX3 protein pod kontrolom efektivnog promotera, kao i sekvencu nukleotida šifrovanu za selektovani marker, koji suštinski ima sekvencu SEQ ID 1 za transformisanje rekombinantne ljudske host ćelije koja već može da eksprimira humani dugi pentraksin PTX3 protein, gde je rekombinantna ljudska host ćelija rekombinantni klon 293F/PTX3/2F12 de ponovan u ECACC pod brojem 08011001.
2.Upotreba vektora, prema zahtevu 1, gde je vektor linearizovan.
3.Transformisana rekombinantna ljudska ćelija koja može da eksprimira humani dugi pentraksin PTX3 protein koji je rekombinantni klon 293F/PTX3/2F12 de ponovan u ECACC pod brojem 08011001 dalje transformisana upotrebom vektora, prema zahtevu 1.
4.Transformisana rekombinantna ljudska ćelija, prema zahtevu 3, koja je rekombinantni klon MS24PTX deponovan u Agenciji za zaštitu zdravlja, Centar za prikupljanje kultura za hitnu pripremu i reakciju (Health Protection Agency, Culture Collections Centre For Emergency Preparedness and Response Salisbury UK), pod brojem 09072902.
5.Upotreba transformisane rekombinantne ljudske ćelije, prema zahtevi ma 3 i 4, za dobijanje humanog dugog pentraksin PTX3 proteina.
6. Postupak za dobijanje humanog dugog pentraksin PTX3 proteina, koji se sastoji od: a) transfekcije rekombinantne ljudske ćelije koja već može da eksprimira rekombinantni humani dugi pentraksin PTX3 protein, sa plazmidom gde je humani dugi pentraksin gen pod kontrolom CMV promotera; b) selektovanja i uzgajanja transfektovane rekombinantne ljudske ćelije; c) prečišćavanja humanog dugog pentraksin PTX3 proteina iz kulture transfektovane ljudske ćelije, pri čemu je rekombinantna ljudska ćelija koja već ekspri mira re kom bina ntn i humani dugi pentraksin PTX3 protein rekombinantni 293F/PTX3/2F12 klon deponovan u ECACC pod brojem 08011001.
7.Postupak prema zahtevu 6, gde jetra nsfektova na ljudska će lij a kl on MS24PTX deponovan u Agenciji za zaštitu zdravlja, Centar za prikupljanje kultura za hitnu pripremu i reakciju (Health Protection Agency, Culture Collections Centre For Emergency Preparedness and Response Salisbury UK), pod brojem 09072902.
8. Postupak prema zahtevima 6 ili 7, gde faza prečišćavanja uključuje najmanje jedan od sledećih koraka: jon-izmenjivačku hromatografiju, hidroksiapatit hromatografiju ili hromatografiju isključivanja po veličini.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09166759 | 2009-07-29 | ||
| PCT/EP2010/060469 WO2011012496A1 (en) | 2009-07-29 | 2010-07-20 | Improved human long pentraxin 3 expression system and uses thereof |
| EP10740573.0A EP2459721B1 (en) | 2009-07-29 | 2010-07-20 | Improved human long pentraxin 3 expression system and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ME01566B true ME01566B (me) | 2014-09-20 |
Family
ID=41120115
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MEP-2013-126A ME01566B (me) | 2009-07-29 | 2010-07-20 | POBOLJŠANI HUMANI DUGI PENTRAKSIN 3 EKSPESIONI SISTEM l NJEGOVE POTREBE |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US8883446B2 (me) |
| EP (1) | EP2459721B1 (me) |
| JP (1) | JP5859963B2 (me) |
| KR (1) | KR101767695B1 (me) |
| CN (1) | CN102549160B (me) |
| AU (1) | AU2010277786B2 (me) |
| BR (1) | BR112012001980A2 (me) |
| CA (1) | CA2769257C (me) |
| CY (1) | CY1116486T1 (me) |
| DK (1) | DK2459721T3 (me) |
| EA (1) | EA023191B1 (me) |
| ES (1) | ES2432242T3 (me) |
| HR (1) | HRP20130947T1 (me) |
| IL (1) | IL217696A (me) |
| ME (1) | ME01566B (me) |
| MX (1) | MX2012001109A (me) |
| PL (1) | PL2459721T3 (me) |
| PT (1) | PT2459721E (me) |
| RS (1) | RS53006B (me) |
| SG (1) | SG177756A1 (me) |
| SI (1) | SI2459721T1 (me) |
| WO (1) | WO2011012496A1 (me) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9206433B2 (en) * | 2013-05-01 | 2015-12-08 | Dna Twopointo, Inc. | Methods, compositions and kits for a one-step DNA cloning system |
| US10253321B2 (en) | 2013-05-01 | 2019-04-09 | Dna2.0, Inc. | Methods, compositions and kits for a one-step DNA cloning system |
| TWI528969B (zh) * | 2013-06-07 | 2016-04-11 | 國立成功大學 | 胺基酸序列用於製備抑制ｐｔｘ３治療鼻咽癌之醫藥組合物之用途 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1298487B1 (it) | 1997-12-19 | 2000-01-10 | Sigma Tau Ind Farmaceuti | Composizioni farmaceutiche comprendenti pentraxina lunga ptx3 per la terapia di patologie di tipo infettivo, infiammatorio o tumorale, |
| EP2311962B1 (en) * | 2002-03-29 | 2013-03-13 | XOMA Technology Ltd. | Multigenic vectors plasmids and methods for increasing expression of recombinant polypeptides |
| EP1832295A1 (en) * | 2006-03-10 | 2007-09-12 | Tecnogen S.P.A. | Use of PTX3 for the treatment of viral diseases |
| WO2009095403A1 (en) * | 2008-01-29 | 2009-08-06 | Tecnogen S.P.A. | Expression system and uses thereof for the production of human long pentraxin 3 |
-
2010
- 2010-07-20 DK DK10740573.0T patent/DK2459721T3/da active
- 2010-07-20 SI SI201030362T patent/SI2459721T1/sl unknown
- 2010-07-20 BR BRBR112012001980-5A patent/BR112012001980A2/pt not_active Application Discontinuation
- 2010-07-20 CN CN201080034030.4A patent/CN102549160B/zh not_active Expired - Fee Related
- 2010-07-20 US US13/387,784 patent/US8883446B2/en not_active Expired - Fee Related
- 2010-07-20 EP EP10740573.0A patent/EP2459721B1/en active Active
- 2010-07-20 ME MEP-2013-126A patent/ME01566B/me unknown
- 2010-07-20 WO PCT/EP2010/060469 patent/WO2011012496A1/en not_active Ceased
- 2010-07-20 KR KR1020127003628A patent/KR101767695B1/ko not_active Expired - Fee Related
- 2010-07-20 PL PL10740573T patent/PL2459721T3/pl unknown
- 2010-07-20 ES ES10740573T patent/ES2432242T3/es active Active
- 2010-07-20 JP JP2012522103A patent/JP5859963B2/ja not_active Expired - Fee Related
- 2010-07-20 SG SG2012005518A patent/SG177756A1/en unknown
- 2010-07-20 PT PT107405730T patent/PT2459721E/pt unknown
- 2010-07-20 EA EA201200198A patent/EA023191B1/ru not_active IP Right Cessation
- 2010-07-20 CA CA2769257A patent/CA2769257C/en not_active Expired - Fee Related
- 2010-07-20 RS RS20130466A patent/RS53006B/sr unknown
- 2010-07-20 AU AU2010277786A patent/AU2010277786B2/en not_active Ceased
- 2010-07-20 MX MX2012001109A patent/MX2012001109A/es active IP Right Grant
- 2010-07-20 HR HRP20130947AT patent/HRP20130947T1/hr unknown
-
2012
- 2012-01-23 IL IL217696A patent/IL217696A/en not_active IP Right Cessation
-
2013
- 2013-10-25 CY CY20131100942T patent/CY1116486T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EA023191B1 (ru) | 2016-05-31 |
| AU2010277786B2 (en) | 2016-04-14 |
| CA2769257C (en) | 2017-12-05 |
| HK1172649A1 (en) | 2013-04-26 |
| CA2769257A1 (en) | 2011-02-03 |
| EP2459721A1 (en) | 2012-06-06 |
| ES2432242T3 (es) | 2013-12-02 |
| SG177756A1 (en) | 2012-02-28 |
| JP5859963B2 (ja) | 2016-02-16 |
| SI2459721T1 (sl) | 2013-11-29 |
| AU2010277786A1 (en) | 2012-03-01 |
| MX2012001109A (es) | 2012-03-26 |
| RS53006B (sr) | 2014-04-30 |
| US20120301873A1 (en) | 2012-11-29 |
| DK2459721T3 (da) | 2013-11-04 |
| IL217696A (en) | 2017-06-29 |
| WO2011012496A1 (en) | 2011-02-03 |
| EA201200198A1 (ru) | 2012-06-29 |
| JP2013504304A (ja) | 2013-02-07 |
| KR101767695B1 (ko) | 2017-08-11 |
| CN102549160A (zh) | 2012-07-04 |
| KR20120047259A (ko) | 2012-05-11 |
| CY1116486T1 (el) | 2017-03-15 |
| BR112012001980A2 (pt) | 2015-09-01 |
| PT2459721E (pt) | 2013-10-31 |
| PL2459721T3 (pl) | 2014-01-31 |
| CN102549160B (zh) | 2014-04-02 |
| EP2459721B1 (en) | 2013-09-18 |
| US8883446B2 (en) | 2014-11-11 |
| HRP20130947T1 (hr) | 2013-11-08 |
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