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    The 4D Nucleome (4DN) program, funded by the National Institutes of Health Common Fund, was established in 2015 to map the three-dimensional organization of the nucleus in space and time (the 4th dimension).

    Image: Frank Alber and Ye Wang

  • Collection |

    The 2025 Nobel Prize in Chemistry has been awarded to Susumu Kitagawa, Richard Robson and Omar M. Yaghi “for the development of metal–organic frameworks.”

    Image: Springer Nature/The Nobel Foundation/Imagesource

  • Collection |

    In recent years, the field of stem cell and developmental biology has seen remarkable breakthroughs that have deepened our understanding of how organisms grow, age and regenerate, with major implications for medicine and biotechnology.

    Image: Dr. Christopher Thomas, Marseille Developmental Biology Institute (IBDM), CNRS & Aix-Marseille Université

  • Collection |

    A collection of protocols covering computational and imaging-based approaches for the identification and visualization of diagnostically relevant targets and features in clinical samples.

    Image: Bishop, K.W., et al., Nat Protoc 19, 1122–1148 (2024).

  • Collection |

    A collection of protocols covering sample preparation and imaging for use with electron microscopy and electron tomography approaches.

    Image: Müller, A. et al., Nat Protoc 19, 1436–1466 (2024).

  • Collection |

    A collection of protocols covering the surgical implantation of imaging windows and the acquisition of intravital imaging data.

    Image: Dawson, C.A. et al., Nat Protoc 16, 1907–1935 (2021).

  • Collection |

    A collection of Protocols and Tutorials covering a range of microscopy approaches, and including the build of microscopy setups and the use of microscopy systems.

    Image: Yao, P. et al., Nat Protoc 18, 3732–3766 (2023).

  • Collection |

    A collection of Protocols covering a wide variety of spatial imaging and spatial omics approaches. The collection aims to facilitate the adoption of high dimensional imaging methods in biological tissues.

    Image: Windhager, J. et al., Nat Protoc 18, 3565–3613 (2023).

  • Collection |

    A collection of Protocols covering 2D and 3D super-resolution imaging methods applied to the profiling of cells in biological tissues, yeast, and plants

    Image: Arizono, M. et al., Nat Protoc (2025).

  • Collection |

    To celebrate the 2024 Nobel Prize in Chemistry awarded for computational protein design and protein structure prediction, we have compiled a selection of our Protocols and Tutorials showcasing the diverse applications of computational structure prediction methods. The articles cover a wide range of topics including protein structure prediction and refinement, docking, predicting intrinsic disorder in proteins, virtual screening, strategies to analyse off-target effects of programmable nucleases, antibody modeling and epitope mapping.

    Image: Du, Z., Su, H., Wang, W. et al. The trRosetta server for fast and accurate protein structure prediction. Nat Protoc 16, 5634–5651 (2021). https://doi.org/10.1038/s41596-021-00628-9

  • Collection |

    The 2024 Nobel Prize in Chemistry has been awarded to David Baker “for computational protein design” and to Demis Hassabis and John M. Jumper “for protein structure prediction”.

    Image: Springer Nature/The Nobel Foundation/Imagesource

  • Collection |

    The Nobel Prize in Physics 2024 has been awarded to John J. Hopfield and Geoffrey E. Hinton “for foundational discoveries and inventions that enable machine learning with artificial neural networks”.

    Image: Springer Nature/The Nobel Foundation/Imagesource

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