This is a protocol for the generation of pediatric patient-derived ependymoma and medulloblastoma tumoroids from surgically resected patient tumor tissues or xenografts, including their use in drug screening and in downstream analyses.

This step-by-step protocol describes a versatile approach for assessing genome-wide off-target activity of diverse genome editors by tracking replication protein A—a key protein that binds single-stranded DNA intermediates.

Residue interaction networks (RINs) describe noncovalent molecular interactions within and between proteins. This Protocol uses RING software to generate these networks from protein structures toward understanding protein structure and function.

This Protocol generates mouse mammary organoids with the tree-like architecture and cellular heterogeneity of native tissue, which recapitulate mammary physiology across the estrous cycle, pregnancy, lactation and involution.

This protocol uses Reprogrammable Adenosine Deaminase Acting on RNA (ADAR) Sensors (RADARS) to robustly sense RNA transcripts inside eukaryotic cells, enabling detection of changes in gene expression or targeting and perturbation of specific mammalian cell types and states.

This Protocol describes the transcriptome-wide labeling of RNAs in a particular subcellular compartment using proximity biotinylation by localized APEX2 enzyme and subsequent enrichment and sequencing of these transcripts.

This is a protocol for the generation of pediatric patient-derived ependymoma and medulloblastoma tumoroids from surgically resected patient tumor tissues or xenografts, including their use in drug screening and in downstream analyses.

Electrocatalytic reactions can be used to upcycle nitrate/nitrite to ammonia, NH₂OH, N₂H₄ and organonitrogen compounds. This protocol describes catalyst screening and reaction optimization via in situ and operando analysis.

This step-by-step protocol describes a versatile approach for assessing genome-wide off-target activity of diverse genome editors by tracking replication protein A—a key protein that binds single-stranded DNA intermediates.

This is a protocol for the preparation and characterization of the activatable BTPE-NO₂@F127 nanoprobe and its applications in multispectral optoacoustic and near-infrared region II fluorescence dual-mode imaging of liver injury models in mice.

This protocol provides guidelines for using fluorescent RNAs, entities consisting of an RNA aptamer bound to its cognate fluorogenic dye, for live imaging of the localization and dynamics of different RNA species in bacteria and mammalian cells.

Residue interaction networks (RINs) describe noncovalent molecular interactions within and between proteins. This Protocol uses RING software to generate these networks from protein structures toward understanding protein structure and function.

This Protocol generates mouse mammary organoids with the tree-like architecture and cellular heterogeneity of native tissue, which recapitulate mammary physiology across the estrous cycle, pregnancy, lactation and involution.