This is a collection of scripts and instructions on how to analyzing amplicon sequence data (i.e., 16S, ITS2, & other marker genes). I created this workflow to create a consistent set of methods for analyzing amplicon sequence data, from when you first receive the sequence data to statistical analyses & data visualization. All you need is to have the latest version of R installed, some experience with the command line & shell, and enough memory to run all of the programs. There are also instructions provided in case you are running these analyses via a computing cluster/Slurm workload manager.
You can choose to go through the workflow using either an Rmd script, an html file, or a PDF, or via the homepage link provided.
If you have questions or concerns, please don't hesitate to reach out. Thanks!
Project Samples
