demuxbyname.sh Exception in thread "main" java.lang.AssertionError: 22, 23, '^@'=0
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#77 demuxbyname.sh Exception in thread "main" java.lang.AssertionError: 22, 23, '^@'=0
Hello,
after executing following code:
java -ea -Xmx54640m -Xms54640m -cp /data/ubbiomed/dkedra/.conda/envs/bbmap/opt/bbmap-39.26-0/current/ jgi.DemuxByName2 in=malmng_05_ptu1__chip_H3K36ac.rep000.r1.fq.gz in2=malmng_05_ptu1__chip_H3K36ac.rep000.r2.fq.gz out=malmng_05_ptu1__chip_H3K36ac.rep_00.split..%.r1.fq.gz out2=malmng_05_ptu1__chip_H3K36ac.rep_00.split..%.r2.fq.gz names=malmng_05_ptu1__chip_H3K36ac.read_prefixes.list replace=:_
Executing jgi.DemuxByName2 [in=malmng_05_ptu1__chip_H3K36ac.rep000.r1.fq.gz, in2=malmng_05_ptu1__chip_H3K36ac.rep000.r2.fq.gz, out=malmng_05_ptu1__chip_H3K36ac.rep_00.split..%.r1.fq.gz, out2=malmng_05_ptu1__chip_H3K36ac.rep_00.split..%.r2.fq.gz, names=malmng_05_ptu1__chip_H3K36ac.read_prefixes.list, replace=:_]
Set INTERLEAVED to false
I got following error:
Exception in thread "main" java.lang.AssertionError: 22, 23, ''=0
LH00348:281:22JL32LT4:5
at barcode.BarcodeStats.loadBarcodeSet(BarcodeStats.java:94)
at jgi.DemuxByName2.handleModeAndNames(DemuxByName2.java:418)
at jgi.DemuxByName2.<init>(DemuxByName2.java:173)
at jgi.DemuxByName2.main(DemuxByName2.java:71)
This is unexpected, as the both input files did pass
fastq_info malmng_05_ptu1__chip_H3K36ac.rep000.r1.fq.gz malmng_05_ptu1__chip_H3K36ac.rep000.r
2.fq.gz
bbmap-39.26-0, installed using conda, CPU: AMD EPYC
Many thanks for your help
Darek Kedra