Search Results for "pcr"
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Showing 88 open source projects for "pcr"
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DeepVariant
DeepVariant is an analysis pipeline that uses a deep neural networks
...DeepTrio extends DeepVariant's functionality, allowing it to utilize the power of neural networks to predict genomic variants in trios or duos. See this page for more details and instructions on how to run DeepTrio. Out-of-the-box use for PCR-positive samples and low quality sequencing runs, and easy adjustments for different sequencing technologies and non-human species. -
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pydna
Clone with Python! Data structures for double stranded DNA
Clone with Python! Data structures for double stranded DNA & simulation of homologous recombination, Gibson assembly, cut & paste cloning. Planning genetic constructs with many parts and assembly steps, such as recombinant metabolic pathways, are often difficult to properly document as is evident from the poor state of documentation in the scientific literature. The pydna python package provide a human-readable formal description of cloning and genetic assembly strategies in Python which... -
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Gemi
PCR primers / probes design from multiple & degenerate sequences
...https://sites.google.com/view/hsa23/ Gemi, an automated, fast, and easy-to-use bioinformatics tool with a user-friendly interface to design primers and probes for polymerase chain reaction (PCR). Gemi accepts multiple aligned and long DNA and RNA sequences with degenerate nucleotide (non-A/C/G/T bases). Gemi can be used for quantitative, real-time and conventional PCR (qPCR, rt-PCR, etc.), and Sanger sequencing. Gemi can parse large dataset of sequences efficiently. Python codes can be supported upon request. Article: Gemi: PCR primers prediction from multiple alignments Comparative and functional genomics 2012;2012:783138 DOI: https://doi.org/10.1155/2012/783138 PMID: https://www.ncbi.nlm.nih.gov/pubmed/23316117 PMCID: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3535827/ -
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FullSSR
SSR detection and primer designing software
...Nowadays is easier and cheaper to obtain genomic data from next generation sequencing. Then, at least two software need to be applied in order to obtain SSR primers of SSR flanking sequences for PCR assays. Here we propose new software that allows the user to work with several sequences for PCR primer design in one step. -
Windocks - Docker Oracle and SQL Server ContainersWindocks is a leader in cloud native database DevOps, recognized by Gartner as a Cool Vendor, and as an innovator by Bloor research in Test Data Management. Novartis, DriveTime, American Family Insurance, and other enterprises rely on Windocks for on-demand database environments for development, testing, and DevOps. Windocks software is easily downloaded for evaluation on standard Linux and Windows servers, for use on-premises or cloud, and for data delivery of SQL Server, Oracle, PostgreSQL, and MySQL to Docker containers or conventional database instances.
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DVB Inspector
DVB transport stream analyzer written in java
DVB Inspector is a java program to analyze captured DVB-streams. It is not a real time tool that can interact with PC-TV DVB hardware directly. You have to use another program to record a Transport stream. It can show the logical structure of the DVB SI and PSI data. It can also show bit rate usage data. Support for for DSM-CC Object carousels, teletext, DVB subtitles Version control has moved to https://github.com/EricBerendsen/dvbinspector . New versions will be available for download... -
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CCE5
Plataforma de Control de Calidad
Plataforma para el Control de Calidad de Diagnóstico en Serología y PCR para la enfermedad de Chagas -
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OligoYap Portable
Oligo design and bioinformatic analysis software.
OligoYap can be used to design classical PCR, SYBR Green, TaqMan, MGB, Molecular Beacons, Microarray and Real-Time PCR primers/probes and for bioinformatics analysis. It has the features of enzyme cut analysis, mutation analysis, DNA/protein match and BLAST. You can easily record laboratory materials/files, prepare PCR mix and many other laboratory operations. It works on windows8.1 and earlier operating systems, but has not been tried on windows10 and later. ... -
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Nitro Secure Module library
Provides Nitro Enclaves with attestation capability
...This is a collection of helpers which Nitro Enclaves userland applications can use to communicate with a connected NitroSecureModule (NSM) device. Various operations can be requested such as PCR query and manipulation, attestation, entropy. -
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covid19
Tokyo COVID-19 Task Force website
New coronavirus infectious disease control site. In Tokyo, in order to centrally announce the infection status in Tokyo, we received reports of outbreaks not only in the jurisdiction of the Tokyo Health Center but also in the cities with health centers (Machida City, Hachioji City) and 23 wards, and aggregated these. In Tokyo, we have set seven monitoring items for the new coronavirus infection, with the two pillars of "infection status" and "medical care provision system" in Tokyo. Every... -
E-commerce Fulfillment For Scaling BrandsFlowspace delivers fulfillment excellence by pairing powerful software and on-the-ground logistics know-how. Our platform provides automation, real-time control, and reliability beyond traditional 3PL capabilities—so you can scale smarter, faster, and easier.
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Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl. Developers/testers/documenters needed.
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GMATA software for Genomic SSR marker
Genome-wide Microsatellite Analyzing Toward Application: GMATA
What is software GMATA v21 Genome-wide Microsatellite Analyzing Toward Application (GMATA) is a software for Simple Sequence Repeats (SSR) analyses, and SSR marker designing and mapping in any DNA sequences. It has the following functions: 1. SSR mining; 2. Statistical analysis and plotting; 3. SSR loci graphic viewing; 4. Marker designing; 5. Electronic mapping and marker transferability investigation. GMATA is accurate, sensitive and fast. It was designed to process large genomic...">
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miRprimer
Automatic design of primers for miR-specific RT-qPCR
miRprimer designs primers for PCR amplification of microRNAs as described (Busk (2014). A tool for design of primers for microRNA-specific quantitative RT-qPCR. BMC Bioinformatics. 15, 29) for use with the method miR-specific RT-qPCR (Cirera, S., and Busk, P.K. (2014). Quantification of miRNAs by a simple and specific qPCR method. Methods in Molecular Biology. 1182, 73-81.). -
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WellMeth
Genome-Wide DNA Methylation Analysis with RRBS
WellMeth is a integrated framework for Reduced Representation Bisulfite-Seq analysis -
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DEBay
Deconvolutes qPCR data to estimate cell-type-specific gene expression
...The user manual of DEBay: https://sourceforge.net/projects/debay/files/UserManual.pdf Sample data: https://sourceforge.net/projects/debay/files/Test_data/ Citation Information: Vimalathithan Devaraj, Biplab Bose. DEBay: A computational tool for deconvolution of quantitative PCR data for estimation of cell type-specific gene expression in a mixed population. Heliyon, 2020, 6(7), e04489. https://doi.org/10.1016/j.heliyon.2020.e04489 -
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Qiagen / Roche converter
Converts Qiagen XML-files to Roche Light CSV-Files.
...The converts the Qiagen XML file including the position and sample number to a txt file which than can be importet into the Sample Sheet of the Roche LightCycler/Cobas 480 Software. It's very convinient if you are working with microtitier plates and high-throughput Coronavirus-PCR. No instalation required. Just drop you XML file into the folder "in_qiagen" and execute the qiagen_roche.exe file. The resulting Roche-file can be found in "out_lc480". *Notice that the folder "in_qiagen" and "out_lc480" must be in the same directory as the qiagen_roche.exe file The qiagen_roche.c contains the sourcecode of the program. ... -
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pcr-efficiency
Easy-to-use web interface to predict PCR efficiencies
pcrEfficiency provides an easy-to-use web interface predicting PCR efficiencies prior to web lab experiments thus easing quantitative real-time PCR set-up. A web-based service as well the source code are provided freely at http://srvgen.upct.es/efficiency.html under the GPL v2 license. The peer-reviewed article describing the tool can be freely accessed at http://www.biomedcentral.com/1471-2105/12/404 . -
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The SPInDel workbench is a computational platform to facilitate the planning and management of SPInDel projects, alignment of nucleotide sequences, visualization and selection of conserved regions, calculation of PCR primers properties, prediction of SPInDel profiles and diverse statistical and phylogenetic analyses. It includes a large dataset comprising nearly 1,800 numeric profiles for the identification of eukaryotic, prokaryotic and viral species.
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RDML stands for Real-time PCR Data Markup Language and is the standard file format for export, storage and exchange of quantitative PCR (qPCR) data. This project focuses on the development, implementation and enhancement of the RDML file format and its description (XML schema).
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GM calculator
for a geometric mean size of a given particle size distribution
...For more detail, please see and cite our following paper: Yamamoto, N., Nazaroff, W.W., Peccia, J. (2014). Assessing the aerodynamic diameters of taxon-specific fungal bioaerosols by quantitative PCR and next-generation DNA sequencing, Journal of Aerosol Science 78, 1-10. -
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PrimerSeq
Designs primers on flanking constitutive exons for RT-PCR
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MPprimer
a program for reliable multiplex PCR primer design
Background: Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in clinical and environmental microbiology studies. However, primer design for multiplex PCR is still a challenging problem and several factors need to be considered. -
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UMI-Reducer
collapsing technical duplicates via Unique Molecular Identifiers
Every sequencing library contains duplicate reads. While many duplicates arise during polymerase chain reaction (PCR), some of these duplicates derive from multiple identical fragments of mRNA present in the original lysate (termed "biological duplicates"). Unique Molecular Identifiers (UMIs) are random oligonucleotide sequences that allow differentiation between technical and biological duplicates. Here we report the development of UMI-Reducer, a new computational tool for processing and differentiating PCR duplicates from biological duplicates. ...