ZA931978B - Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plants - Google Patents
Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plantsInfo
- Publication number
- ZA931978B ZA931978B ZA931978A ZA931978A ZA931978B ZA 931978 B ZA931978 B ZA 931978B ZA 931978 A ZA931978 A ZA 931978A ZA 931978 A ZA931978 A ZA 931978A ZA 931978 B ZA931978 B ZA 931978B
- Authority
- ZA
- South Africa
- Prior art keywords
- seeds
- lysine
- plants
- increasing
- methods
- Prior art date
Links
- 239000004472 Lysine Substances 0.000 title 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 title 1
- 239000004473 Threonine Substances 0.000 title 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
- C12N15/8254—Tryptophan or lysine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85541492A | 1992-03-19 | 1992-03-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA931978B true ZA931978B (en) | 1994-09-19 |
Family
ID=25321215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA931978A ZA931978B (en) | 1992-03-19 | 1993-03-19 | Nucleic acid fragments and methods for increasing the lysine and threonine content of the seeds of plants |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP1394257B1 (xx) |
| JP (1) | JPH07504821A (xx) |
| AU (1) | AU675933B2 (xx) |
| CA (1) | CA2132414C (xx) |
| DE (2) | DE69332974T2 (xx) |
| DK (2) | DK0640141T3 (xx) |
| MX (1) | MX9301521A (xx) |
| WO (1) | WO1993019190A1 (xx) |
| ZA (1) | ZA931978B (xx) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7705215B1 (en) * | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5550318A (en) * | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| CA2074355C (en) | 1990-01-22 | 2008-10-28 | Ronald C. Lundquist | Method of producing fertile transgenic corn plants |
| US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
| ES2176208T3 (es) * | 1992-10-05 | 2002-12-01 | Univ North Carolina State | Metodo para incrementar la expresion y para reducir la variabilidad de expresion de genes foraneos en celulas vegetales. |
| DE69330518T2 (de) * | 1992-11-10 | 2002-05-08 | Ajinomoto Co., Inc. | Dna, die aspartokinase iii-mutanten kodiert und ihre verwendung zur herstellung von l-threonin durch fermentation |
| CA2161881C (en) * | 1993-01-13 | 2001-03-27 | A. Gururaj Rao | High lysine derivatives of alpha-hordothionin |
| US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
| US6326527B1 (en) | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| DE69434786T2 (de) * | 1993-11-30 | 2007-06-14 | E.I. Du Pont De Nemours And Co., Wilmington | Chimaere gene und verfahren zur steigerung des lysin-gehalts der samen von mais, soja und raps pflanzen |
| JPH07155184A (ja) * | 1993-12-08 | 1995-06-20 | Ajinomoto Co Inc | 発酵法によるl−リジンの製造法 |
| IL113685A0 (en) * | 1994-05-13 | 1995-08-31 | Du Pont | Nucleic acid fragments chimeric genes and methods for increasing the methionine content of the seeds of plants |
| CA2192550A1 (en) * | 1994-07-08 | 1996-01-25 | Saverio Carl Falco | Chimeric genes and method for increasing the threonine content of the seeds of plants |
| CN1195377A (zh) * | 1995-05-31 | 1998-10-07 | 先锋高级育种国际公司 | 在种子中增加必需氨基酸积累的方法 |
| MX9709352A (es) * | 1995-06-02 | 1998-02-28 | Pioneer Hi Bred Int | Derivados de alfa-hortodionina con alto contenido de metionina. |
| HUP9900876A2 (hu) * | 1995-06-02 | 1999-07-28 | Pioneer Hi-Bred International, Inc. | Az alfa-hordotionin nagy treonintartalmú származékai |
| JP4004065B2 (ja) * | 1995-06-13 | 2007-11-07 | 味の素株式会社 | 発酵法によるl−リジンの製造法 |
| US6166292A (en) * | 1996-04-26 | 2000-12-26 | Ajinomoto Co., Inc. | Raffinose synthetase gene, method of producing raffinose and transgenic plant |
| US6891084B1 (en) | 1996-04-26 | 2005-05-10 | Ajinomoto Co., Inc. | DNA encoding raffinose synthase from soybean |
| JP4088982B2 (ja) * | 1996-10-15 | 2008-05-21 | 味の素株式会社 | 発酵法によるl−アミノ酸の製造法 |
| CA2272564A1 (en) * | 1996-11-18 | 1998-05-28 | Avebe | Transgenic plant or plants with a naturally high water content overproducing at least two amino acids of the aspartate family |
| AU747997B2 (en) * | 1997-03-27 | 2002-05-30 | E.I. Du Pont De Nemours And Company | Chimeric genes and methods for increasing the lysine content of the seeds of plants |
| US6169232B1 (en) | 1997-07-15 | 2001-01-02 | Dow Agrosciences Llc | Nucleotide sequences of genes encoding sink protein and uses thereof for improving the nutritional quality of feeds |
| PT1440151E (pt) * | 2001-09-05 | 2008-03-17 | Monsanto Technology Llc | Promotor 7s alfa específico da semente para expressar genes em plantas |
| WO2005014828A2 (en) | 2003-08-01 | 2005-02-17 | Basf Plant Science Gmbh | Process for the production of fine chemicals in plants |
| US8008545B2 (en) | 2003-04-15 | 2011-08-30 | Basf Plant Science Gmbh | Process for the production of fine chemicals |
| US7157281B2 (en) * | 2003-12-11 | 2007-01-02 | Monsanto Technology Llc | High lysine maize compositions and event LY038 maize plants |
| US7855323B2 (en) | 2004-02-10 | 2010-12-21 | Monsanto Technology Llc | Recombinant DNA for gene suppression |
| US7951565B2 (en) | 2004-04-06 | 2011-05-31 | Metanomics Gmbh | Process for the production of fine chemicals |
| CA2559760A1 (en) | 2004-07-02 | 2006-07-06 | Metanomics Gmbh | Process for the production of fine chemicals |
| CA2585798A1 (en) | 2004-12-17 | 2006-06-17 | Metanomics Gmbh | Process for the control of production of fine chemicals |
| EP2573188A2 (en) | 2005-03-02 | 2013-03-27 | Metanomics GmbH | Process for the production of fine chemicals |
| WO2006092449A2 (en) | 2005-03-02 | 2006-09-08 | Metanomics Gmbh | Process for the production of fine chemicals |
| RU2460281C2 (ru) * | 2005-10-03 | 2012-09-10 | Монсанто Текнолоджи Ллс | Семена трансгенных растений с повышенным содержанием лизина |
| WO2008034648A1 (en) | 2006-04-05 | 2008-03-27 | Metanomics Gmbh | Process for the production of a fine chemical |
| BRPI0810083B8 (pt) | 2007-03-28 | 2020-10-06 | Monsanto Technology Llc | métodos para estabelecer onde uma planta de soja ou semente de soja deve ser cultivada, de melhoramento da planta de soja, para selecionar uma semente de soja, de distribuição de uma planta de soja, para isolar sementes de soja de maturidade precoce indeterminada, e para determinar se uma planta de soja tem um grupo de maturidade |
| CA2704725C (en) | 2007-09-11 | 2018-01-02 | Monsanto Technology Llc | Increased alpha-prime beta-conglycinin soybeans |
| BRPI0914933B1 (pt) | 2008-06-30 | 2018-09-25 | Monsanto Technology Llc | Polipeptídeo exibindo atividade de aspartato quinase (ak) que não é sujeito a inibição por produto final, polinucleotídeo codificando o mesmo, construto de dna recombinante,microrganismo transformado, produto alimentício, farelo ou farinha, bem como métodos de transformação de uma célula de planta, de produção de uma planta transformada e de aumento do teor de aminoácido livre total de uma planta. |
| CN108486082A (zh) | 2012-10-18 | 2018-09-04 | 中粮生物化学(安徽)股份有限公司 | 天冬氨酸激酶iii突变体及其宿主细胞和应用 |
| CN106086011B (zh) * | 2016-06-18 | 2019-10-18 | 北京大北农科技集团股份有限公司 | 用于检测除草剂耐受性大豆植物dbn9004的核酸序列及其检测方法 |
| CN113201514B (zh) | 2020-10-16 | 2022-09-06 | 中国科学院天津工业生物技术研究所 | 具有天冬氨酸激酶活性的多肽及其在生产氨基酸中的应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5258300A (en) * | 1988-06-09 | 1993-11-02 | Molecular Genetics Research And Development Limited Partnership | Method of inducing lysine overproduction in plants |
| DE3943117A1 (de) * | 1989-12-27 | 1991-07-04 | Forschungszentrum Juelich Gmbh | Verfahren zur fermentativen herstellung von aminosaeure, insbesondere l-lysin, dafuer geeignete mikroorganismen und rekombinante dna |
| US5367110A (en) * | 1990-11-13 | 1994-11-22 | Yeda Research And Development Co. Ltd. | Transgenic plants overproducing threonine and lysine |
| US7746705B2 (en) * | 2007-12-10 | 2010-06-29 | Spansion Llc | Selective application of word line bias to minimize fringe effects in electromagnetic fields during erase of nonvolatile memory |
-
1993
- 1993-03-18 WO PCT/US1993/002480 patent/WO1993019190A1/en not_active Ceased
- 1993-03-18 EP EP03009812A patent/EP1394257B1/en not_active Expired - Lifetime
- 1993-03-18 DK DK93908395T patent/DK0640141T3/da active
- 1993-03-18 MX MX9301521A patent/MX9301521A/es unknown
- 1993-03-18 AU AU39233/93A patent/AU675933B2/en not_active Expired
- 1993-03-18 EP EP93908395A patent/EP0640141B1/en not_active Expired - Lifetime
- 1993-03-18 JP JP5516720A patent/JPH07504821A/ja active Pending
- 1993-03-18 DE DE69332974T patent/DE69332974T2/de not_active Expired - Lifetime
- 1993-03-18 DE DE69334163T patent/DE69334163T2/de not_active Expired - Lifetime
- 1993-03-18 CA CA002132414A patent/CA2132414C/en not_active Expired - Lifetime
- 1993-03-18 DK DK03009812T patent/DK1394257T3/da active
- 1993-03-19 ZA ZA931978A patent/ZA931978B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU675933B2 (en) | 1997-02-27 |
| JPH07504821A (ja) | 1995-06-01 |
| MX9301521A (es) | 1993-09-01 |
| DK0640141T3 (da) | 2003-07-21 |
| CA2132414C (en) | 2009-01-27 |
| DE69332974D1 (de) | 2003-06-18 |
| DE69332974T2 (de) | 2004-05-19 |
| EP1394257B1 (en) | 2007-08-08 |
| DK1394257T3 (da) | 2007-12-10 |
| CA2132414A1 (en) | 1993-09-30 |
| EP0640141B1 (en) | 2003-05-14 |
| AU3923393A (en) | 1993-10-21 |
| EP1394257A1 (en) | 2004-03-03 |
| WO1993019190A1 (en) | 1993-09-30 |
| EP0640141A1 (en) | 1995-03-01 |
| DE69334163D1 (de) | 2007-09-20 |
| DE69334163T2 (de) | 2008-04-30 |
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