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WO2026024468A1 - Récepteurs antigéniques chimériques liés modulaires - Google Patents

Récepteurs antigéniques chimériques liés modulaires

Info

Publication number
WO2026024468A1
WO2026024468A1 PCT/US2025/037092 US2025037092W WO2026024468A1 WO 2026024468 A1 WO2026024468 A1 WO 2026024468A1 US 2025037092 W US2025037092 W US 2025037092W WO 2026024468 A1 WO2026024468 A1 WO 2026024468A1
Authority
WO
WIPO (PCT)
Prior art keywords
chimeric antigen
antigen receptors
linked chimeric
modl
modular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2025/037092
Other languages
English (en)
Inventor
Paul Daniel Donohoue
Brian J. FRANCICA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Caribou Biosciences Inc
Original Assignee
Caribou Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Caribou Biosciences Inc filed Critical Caribou Biosciences Inc
Publication of WO2026024468A1 publication Critical patent/WO2026024468A1/fr
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/421—Immunoglobulin superfamily
    • A61K40/4211—CD19 or B4
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/4214—Receptors for cytokines
    • A61K40/4215—Receptors for tumor necrosis factors [TNF], e.g. lymphotoxin receptor [LTR], CD30
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/10—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterized by the structure of the chimeric antigen receptor [CAR]
    • A61K2239/23—On/off switch
    • A61K2239/24—Dimerizable CARs; CARs with adapter
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Mechanical Treatment Of Semiconductor (AREA)

Abstract

L'invention comprend des récepteurs antigéniques chimériques liés modulaires (MODL-CAR), des cellules exprimant les MODL-CAR, des compositions comprenant les cellules et leurs procédés de fabrication et d'utilisation, y compris des méthodes de traitement faisant appel aux MODL-CAR exprimant des cellules modifiées.
PCT/US2025/037092 2024-07-26 2025-07-10 Récepteurs antigéniques chimériques liés modulaires Pending WO2026024468A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202463676169P 2024-07-26 2024-07-26
US63/676,169 2024-07-26

Publications (1)

Publication Number Publication Date
WO2026024468A1 true WO2026024468A1 (fr) 2026-01-29

Family

ID=96808908

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2025/037092 Pending WO2026024468A1 (fr) 2024-07-26 2025-07-10 Récepteurs antigéniques chimériques liés modulaires

Country Status (1)

Country Link
WO (1) WO2026024468A1 (fr)

Citations (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482858A (en) 1987-05-21 1996-01-09 Creative Biomolecules, Inc. Polypeptide linkers for production of biosynthetic proteins
US5525491A (en) 1991-02-27 1996-06-11 Creative Biomolecules, Inc. Serine-rich peptide linkers
US6410319B1 (en) 1998-10-20 2002-06-25 City Of Hope CD20-specific redirected T cells and their use in cellular immunotherapy of CD20+ malignancies
US6849428B1 (en) 1997-03-05 2005-02-01 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
WO2011104382A1 (fr) 2010-02-26 2011-09-01 Cellectis Utilisation d'endonucléases pour insérer des transgènes dans des locus safe harbor
WO2013098244A1 (fr) 2011-12-30 2013-07-04 Wageningen Universiteit Ribonucléoprotéines cascade modifiées et utilisations de celles-ci
WO2014087010A1 (fr) 2012-12-07 2014-06-12 Ablynx N.V. Polypeptides améliorés dirigés contre ige
US9260752B1 (en) 2013-03-14 2016-02-16 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
US9650617B2 (en) 2015-01-28 2017-05-16 Pioneer Hi-Bred International. Inc. CRISPR hybrid DNA/RNA polynucleotides and methods of use
US10336810B2 (en) 2015-02-12 2019-07-02 University Health Network Chimeric antigen receptors, encoding nucleic acids and methods of use thereof
WO2019241452A1 (fr) 2018-06-13 2019-12-19 Caribou Biosciences, Inc. Composants en cascade modifiés et complexes en cascade
WO2020150339A1 (fr) 2019-01-16 2020-07-23 Caribou Biosciences, Inc. Anticorps bcma humanisé et cellules bcma-car-t
WO2020206162A1 (fr) 2019-04-03 2020-10-08 Regeneron Pharmaceuticals, Inc. Procédés et compositions pour l'insertion de séquences de codage d'anticorps dans un locus d'hébergement sûr
US20200370067A1 (en) 2019-05-21 2020-11-26 University Of Washington Method to identify and validate genomic safe harbor sites for targeted genome engineering
WO2021050857A1 (fr) 2019-09-13 2021-03-18 Memorial Sloan-Kettering Cancer Center Anticorps anti-cd371 et leurs utilisations
US11066657B2 (en) 2014-10-28 2021-07-20 Agrivida, Inc Methods and compositions for stabilizing trans-splicing intein modified proteases
WO2021152086A1 (fr) 2020-01-30 2021-08-05 Bit Bio Limited Loci d'hebergement sûrs
US11091541B2 (en) 2013-04-29 2021-08-17 Hoffmann-La Roche Inc. Human FcRn-binding modified antibodies and methods of use
US11104375B2 (en) 2019-04-02 2021-08-31 Denso Corporation Rotary electric machine control device and electric power steering apparatus using the same
WO2022086846A2 (fr) 2020-10-19 2022-04-28 Caribou Biosciences, Inc. Polynucléotides et guides contenant de l'adn pour systèmes crispr de type v, et leurs méthodes de fabrication et d'utilisation
US20220348929A1 (en) 2019-12-09 2022-11-03 Caribou Biosciences, Inc. Crispr abasic restricted nucleotides and crispr accuracy via analogs
WO2023230447A1 (fr) 2022-05-24 2023-11-30 Caribou Biosciences, Inc. Anticorps anti-ror1 et cellules modifiées ciblant ror1
US20230416747A1 (en) 2020-10-26 2023-12-28 Arsenal Biosciences, Inc. Safe harbor loci
WO2024097763A1 (fr) * 2022-11-01 2024-05-10 Memorial Sloan-Kettering Cancer Center Système de tri reposant sur l'intéine et polypeptides chimériques modulaires

Patent Citations (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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US5525491A (en) 1991-02-27 1996-06-11 Creative Biomolecules, Inc. Serine-rich peptide linkers
US6849428B1 (en) 1997-03-05 2005-02-01 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
US6410319B1 (en) 1998-10-20 2002-06-25 City Of Hope CD20-specific redirected T cells and their use in cellular immunotherapy of CD20+ malignancies
WO2011104382A1 (fr) 2010-02-26 2011-09-01 Cellectis Utilisation d'endonucléases pour insérer des transgènes dans des locus safe harbor
WO2013098244A1 (fr) 2011-12-30 2013-07-04 Wageningen Universiteit Ribonucléoprotéines cascade modifiées et utilisations de celles-ci
WO2014087010A1 (fr) 2012-12-07 2014-06-12 Ablynx N.V. Polypeptides améliorés dirigés contre ige
US9260752B1 (en) 2013-03-14 2016-02-16 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
US11091541B2 (en) 2013-04-29 2021-08-17 Hoffmann-La Roche Inc. Human FcRn-binding modified antibodies and methods of use
US11066657B2 (en) 2014-10-28 2021-07-20 Agrivida, Inc Methods and compositions for stabilizing trans-splicing intein modified proteases
US9650617B2 (en) 2015-01-28 2017-05-16 Pioneer Hi-Bred International. Inc. CRISPR hybrid DNA/RNA polynucleotides and methods of use
US10336810B2 (en) 2015-02-12 2019-07-02 University Health Network Chimeric antigen receptors, encoding nucleic acids and methods of use thereof
WO2019241452A1 (fr) 2018-06-13 2019-12-19 Caribou Biosciences, Inc. Composants en cascade modifiés et complexes en cascade
WO2020150339A1 (fr) 2019-01-16 2020-07-23 Caribou Biosciences, Inc. Anticorps bcma humanisé et cellules bcma-car-t
US11104375B2 (en) 2019-04-02 2021-08-31 Denso Corporation Rotary electric machine control device and electric power steering apparatus using the same
WO2020206162A1 (fr) 2019-04-03 2020-10-08 Regeneron Pharmaceuticals, Inc. Procédés et compositions pour l'insertion de séquences de codage d'anticorps dans un locus d'hébergement sûr
US20200370067A1 (en) 2019-05-21 2020-11-26 University Of Washington Method to identify and validate genomic safe harbor sites for targeted genome engineering
WO2021050857A1 (fr) 2019-09-13 2021-03-18 Memorial Sloan-Kettering Cancer Center Anticorps anti-cd371 et leurs utilisations
US20220348929A1 (en) 2019-12-09 2022-11-03 Caribou Biosciences, Inc. Crispr abasic restricted nucleotides and crispr accuracy via analogs
WO2021152086A1 (fr) 2020-01-30 2021-08-05 Bit Bio Limited Loci d'hebergement sûrs
WO2022086846A2 (fr) 2020-10-19 2022-04-28 Caribou Biosciences, Inc. Polynucléotides et guides contenant de l'adn pour systèmes crispr de type v, et leurs méthodes de fabrication et d'utilisation
US20230416747A1 (en) 2020-10-26 2023-12-28 Arsenal Biosciences, Inc. Safe harbor loci
WO2023230447A1 (fr) 2022-05-24 2023-11-30 Caribou Biosciences, Inc. Anticorps anti-ror1 et cellules modifiées ciblant ror1
WO2024097763A1 (fr) * 2022-11-01 2024-05-10 Memorial Sloan-Kettering Cancer Center Système de tri reposant sur l'intéine et polypeptides chimériques modulaires

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