WO2026000382A1 - Poct hematology analyzer and hematology analysis method - Google Patents

Abstract

The present application discloses a POCT hematology analyzer and a hematology analysis method. In the POCT hematology analyzer, a pressure buildup mechanism performs pressure detection on a test chamber during testing of a reagent cartridge; a processor determines, on the basis of the pressure detection result from the pressure buildup mechanism, whether the gas tightness of the test chamber is abnormal. Thus, the reagent cartridge can be processed in a timely manner when the gas tightness of the test chamber is abnormal, thereby reducing damage to the POCT hematology analyzer caused by problems such as gas leakage or port blockage of the reagent cartridge, and improving the test accuracy and reliability of the POCT hematology analyzer.

Description

POCT blood cell analyzer and blood cell analysis methods Technical Field

This application relates to the field of blood analysis technology, and in particular to POCT blood cell analyzers and blood cell analysis methods.

Background Technology

Currently, POCT hematology analyzers typically perform hematology testing using reagent kits. As a necessary consumable for POCT hematology analyzers, the production quality of these kits can have a certain impact on the accuracy of hematology analysis results and the testing process.

During the testing process, POCT hematology analyzers require a pressure-building mechanism to provide a preset pressure to the test cell, ensuring that the sample in the test cell undergoes impedance testing under this pressure environment. If the airtightness of the test cell in the kit is abnormal, such as due to air leakage or blockage, the accuracy of the sample test results during impedance testing will decrease. Alternatively, it may cause leakage of the test waste liquid from the kit, allowing it to enter the instrument and cause damage, thus reducing the accuracy and reliability of the POCT hematology analyzer.

Summary of the Invention

The main technical problem addressed in this application is how to improve the accuracy and reliability of POCT blood analyzers.

To address the aforementioned technical problems, this application provides a first technical solution: a POCT hematology analyzer, comprising a support mechanism, a pressure-building mechanism, a detection mechanism, and a processor. The support mechanism is used to load a reagent kit, and the detection chamber of the reagent kit stores a sample to be tested. The pressure-building mechanism is connected to the reagent kit on the support mechanism and is used to provide a preset pressure to the detection chamber. The detection mechanism is used to test the sample in the detection chamber under the preset pressure. The processor is connected to both the pressure-building mechanism and the detection mechanism. The pressure-building mechanism is also used to perform pressure detection on the detection chamber during the detection process of the detection mechanism, and the processor is used to determine whether the airtightness of the detection chamber is abnormal based on the pressure detection result of the pressure-building mechanism.

The aforementioned testing mechanism is used to test the sample in the aforementioned testing pool under the aforementioned preset pressure within a first testing period. The time for the aforementioned pressure building mechanism to test the pressure in the aforementioned testing pool is a second testing period. The second testing period is within the aforementioned first testing period, and the end time of the aforementioned second testing period is before the end time of the aforementioned first testing period.

The pressure-building mechanism is used to perform pressure detection on the detection pool during the detection process to obtain the real-time pressure value of the detection pool. The processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result between the real-time pressure value and a first set threshold.

The processor is used to generate a first warning message when the real-time pressure value is greater than the first set threshold, so as to indicate that the reagent kit is leaking air through the first warning message.

The pressure-building mechanism is used to perform pressure testing on the detection pool at preset intervals during the detection process to obtain the pressure change value of the detection pool. The processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the pressure change value and a first threshold range.

The first threshold range includes a second set threshold and a third set threshold, wherein the third set threshold is greater than the second set threshold; the processor is used to generate a first warning message when the pressure change value is greater than the third set threshold, so as to indicate that the reagent kit is leaking air through the first warning message, and/or the processor is used to generate a second warning message when the pressure change value is less than the second set threshold, so as to indicate that the reagent kit is clogged through the second warning message.

The pressure building mechanism is used to perform pressure detection on the detection pool during the detection process to obtain a first pressure value. The pressure building mechanism is also used to continue to perform pressure detection on the detection pool after a preset time interval to obtain a second pressure value. The processor is used to calculate the difference between the second pressure value and the first pressure value to obtain the pressure change value.

The pressure change value is the difference between the pressure value of the detection pool during the detection process and the initial pressure value of the detection pool at the start of the test.

The pressure-building mechanism is used to perform pressure testing on the detection pool within a preset time period of the detection process to obtain the rate of change of the pressure value of the detection pool within the preset time period. The processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result between the rate of change and the second threshold range.

The second threshold range includes a fourth set threshold and a fifth set threshold, wherein the fifth set threshold is greater than the fourth set threshold; the processor is used to generate a first warning message when the rate of change is greater than the fifth set threshold, so as to indicate that the reagent kit is leaking air through the first warning message, and/or the processor is used to generate a second warning message when the rate of change is less than the fourth set threshold, so as to indicate that the reagent kit is clogged through the second warning message.

The aforementioned pressure-building mechanism includes a pressure control component, a pressure sensor, and a connector. The connector is disposed on the aforementioned support mechanism. The pressure control component is connected to the connector and the pressure sensor via pipelines. The connector is used to dock with the aforementioned reagent kit so that the pressure control component provides a preset pressure to the aforementioned detection cell. The pressure sensor is used to detect the pressure of the aforementioned detection cell.

The pressure control component includes a pressure-building component, a negative pressure component, a first control component, and a second control component. The first end of the first control component is connected to the connector, the second end of the first control component is connected to the first end of the negative pressure component, the first end of the second control component is connected to the second end of the negative pressure component, and the second end of the second control component is connected to the pressure-building component. The pressure-building component is used to establish negative pressure on the negative pressure component to provide the preset pressure to the detection pool when the first control component is turned on.

The processor is used to disconnect the pressure-building mechanism from the reagent kit when the airtightness of the detection cell is determined to be abnormal.

The detection pool includes a front pool, a rear pool, and a microporous sheet. The front pool is connected to the rear pool via the microporous sheet. The detection mechanism is used to count cells in the sample as it flows from the front pool through the microporous sheet to the rear pool. The processor is used to acquire the flow rate of the sample as it flows through the microporous sheet, and to determine whether the airtightness of the detection pool is abnormal based on the flow rate change during the detection process. Alternatively, the processor is also used to calculate the flow pressure drop of the sample based on the flow rate, and to determine whether the airtightness of the detection pool is abnormal based on the flow pressure drop during the detection process.

To solve the above-mentioned technical problems, this application provides a second technical solution: a blood cell analysis method, comprising: receiving a reagent kit, wherein the detection cell of the reagent kit stores a sample to be tested; performing an impedance test on the sample of the reagent kit; and performing a pressure test on the detection cell during the detection process of the detection mechanism, so as to determine whether the airtightness of the detection cell is abnormal based on the pressure test result of the pressure building mechanism.

The beneficial effects of this application are as follows: Unlike the prior art, the POCT blood cell analyzer of this application uses a pressure-building mechanism to perform pressure detection on the test cell during the test of the reagent kit. The processor judges whether the airtightness of the test cell is abnormal based on the pressure detection result of the pressure-building mechanism, so that the reagent kit can be dealt with in a timely manner when the airtightness of the test cell is abnormal, reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or blockage of the reagent kit, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Attached Figure Description

To more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

Figure 1 is a schematic diagram of an embodiment of the POCT blood cell analyzer provided in this application;

Figure 2 is an operational schematic diagram of an embodiment of the pressure change curve during the normal detection process provided in this application;

Figure 3 is an operational schematic diagram of an embodiment of the pressure change curve during air leakage provided in this application;

Figure 4 is an operational schematic diagram of an embodiment of the pressure change curve during plugging provided in this application;

Figure 5 is a structural schematic diagram of the pressure building mechanism in Figure 1;

Figure 6 is a flowchart illustrating an embodiment of the blood cell analysis method provided in this application.

Embodiments of the present invention

The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be particularly noted that the following embodiments are for illustrative purposes only and do not limit the scope of the application. Similarly, the following embodiments are only some, not all, embodiments of the present application, and all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of the present application.

In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a mutually exclusive, independent, or alternative embodiment. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

In the description of this application, it is necessary to specify that, unless otherwise expressly stated and limited, the terms "installation," "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or a connection through an intermediate medium. Those skilled in the art will understand the specific meanings of the above terms within the context of this application.

Please refer to Figure 1, which is a schematic diagram of the structure of an embodiment of the POCT hematology analyzer provided in this application. As shown in Figure 1, the POCT hematology analyzer of this application embodiment includes a support mechanism 11, a pressure building mechanism 12, a detection mechanism 13, and a processor 14.

The carrier 11 is used to load the reagent kit 110, and the detection cell of the reagent kit 110 stores the sample to be tested; the pressure building mechanism 12 is connected to the reagent kit 110 on the carrier 11, and the pressure building mechanism 12 is used to provide a preset pressure to the detection cell; the detection mechanism 13 is used to test the sample in the detection cell under the preset pressure; the processor 14 is connected to the pressure building mechanism 12, the carrier 11 and the detection mechanism 13 respectively; the pressure building mechanism 12 is also used to perform pressure detection on the detection cell during the detection process of the detection mechanism 13, and the processor 14 is used to determine whether the airtightness of the detection cell is abnormal based on the pressure detection result of the pressure building mechanism 12.

Specifically, the reagent kit 110 may include a detection chamber for storing the sample to be tested, and the detection chamber can serve as a site for sample testing, allowing the detection mechanism 13 to perform at least one of impedance detection and optical detection on the sample in the detection chamber. In a possible implementation, the detection mechanism 13 may include a first detection component and a second detection component. The first detection component is used to perform impedance detection on the sample in the detection chamber, and the second detection component is used to perform optical detection on the sample in the detection chamber. The detection chamber includes a front chamber, a rear chamber, and a microwell sheet. A microwell sheet is provided with a micropore, and the front chamber is connected to the rear chamber through the micropore on the microwell sheet. The sample to be tested is stored in the front chamber. When the reagent kit 110 is loaded on the carrier mechanism 11, the reagent kit 110 is electrically connected to the first detection component, which is used to perform impedance counting on the cells in the sample as the sample in the front chamber flows through the microwell sheet to the rear chamber.

When the reagent kit 110 is loaded onto the carrier 11, the pressure-building mechanism 12 is connected to the reagent kit 110 on the carrier 11, so that the pressure-building mechanism 12 can perform a pressure-building operation on the detection cell of the reagent kit 110, providing a preset pressure to the detection cell. The preset pressure can be a preset positive pressure or a preset negative pressure; for example, the pressure-building mechanism 12 can be connected to the front cell of the detection cell, and the pressure-building mechanism 12 is used to provide a preset positive pressure to the front cell so that the sample in the front cell flows through the micropores to the rear cell; or, the pressure-building mechanism 12 can be connected to the rear cell of the detection cell, and the pressure-building mechanism 12 is used to provide a preset negative pressure to the rear cell so that the sample in the front cell flows through the micropores to the rear cell. No specific limitation is made here.

When the pressure in the detection cell reaches the preset pressure, the pressure-building mechanism 12 stops its pressure-building operation. The detection mechanism 13 performs impedance detection on the sample in the detection cell at the preset pressure, causing the sample in the front cell to flow through the micropores to the rear cell, resulting in a change in the pressure within the detection cell. For example, when the pressure-building mechanism 12 is connected to the front cell of the detection cell, the gas volume in the front cell increases due to the sample flowing to the rear cell, causing the pressure in the front cell to gradually decrease from the preset positive pressure. Alternatively, when the pressure-building mechanism 12 can be connected to the rear cell of the detection cell, the gas volume in the rear cell decreases due to the sample flowing to the rear cell, causing the pressure in the rear cell to gradually increase from the preset negative pressure. That is, during normal testing, since the micropore diameter of the microporous sheet is fixed, the pressure in the detection cell will change with a similar trend. Therefore, the POCT blood cell analyzer of this embodiment can use the pressure-building mechanism 12 to perform pressure detection on the detection cell during the detection process of the detection mechanism 13 to obtain pressure detection results. The processor 14 can determine whether the airtightness of the detection cell is abnormal based on the pressure detection of the pressure-building mechanism 12.

The airtightness of the aforementioned detection pool indicates whether there is any leakage in the front or rear pool during the detection process. Alternatively, airtightness can also indicate the flow between the front and rear pools during the detection process, such as whether there is a situation where the flow between the front and rear pools is blocked due to micropore blockage. When the airtightness of the detection pool is normal, the sample and gas in the front pool can only flow to the rear pool through the micropores of the microporous sheet. When the airtightness of the detection pool is abnormal, depending on the connection position of the pressure building mechanism 12, it may include abnormal pressure drop in the front pool due to leakage or blockage, and abnormal pressure rise in the rear pool due to leakage or blockage, etc., without specific limitations.

In this embodiment, the POCT hematology analyzer uses a pressure-building mechanism 12 to perform pressure detection on the test cell during the detection process of the reagent kit 110. The processor 14 determines whether the airtightness of the test cell is abnormal based on the pressure detection result of the pressure-building mechanism 12. This allows the reagent kit 110 to be dealt with in a timely manner when the airtightness of the test cell is abnormal, reducing the damage to the POCT hematology analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT hematology analyzer.

In one embodiment, the detection mechanism 13 is used to detect the sample in the detection pool under a preset pressure within a first detection duration, and the pressure building mechanism 12 performs pressure detection on the detection pool for a second detection duration. The second detection duration is within the first detection duration, and the end time of the second detection duration is before the end time of the first detection duration.

Specifically, when the testing mechanism 13 tests the sample in the testing pool under a preset pressure, the time spent by the testing mechanism 13 during the testing process is the first testing time, and the time for the pressure-building mechanism 12 to perform pressure testing on the testing pool is the second testing time. The second testing time is within the first testing time, and the end time of the second testing time is before the end time of the first testing time. That is, the pressure-building mechanism 12 can perform testing at any time during the first testing time of the testing mechanism 13, but the pressure-building mechanism 12 cannot perform pressure testing at the end of the testing time of the testing mechanism 13. For example, if the first testing time of the testing mechanism 13 is divided into a first testing segment, a second testing segment, and a third testing segment, the second testing time for the pressure-building mechanism 12 to perform pressure testing can be completed within the first or second testing segment, but the end time of the pressure-building mechanism 12 to perform pressure testing cannot be the same as the end time of the third testing segment.

For example, the testing mechanism 13 tests the sample in the testing pool under the preset pressure within 0 to 14 seconds, and the pressure building mechanism 12 can test the pressure in the testing pool within 0 to 10 seconds, so that there is a time difference between the end time of the pressure building mechanism 12's pressure test and the end time of the testing mechanism 13's sample test.

In this embodiment, by setting the end time of the second detection duration to be before the end time of the first detection duration, the processor 14 can promptly process the reagent kit 110 when the POCT blood cell analyzer determines that the airtightness of the detection pool is abnormal. This prevents the sample in the detection pool from re-entering the connection channel between the pressure building mechanism 12 and the reagent kit 110 due to the abnormal airtightness of the detection pool, thereby reducing the possibility of sample contamination of the connection channel inside the POCT blood cell analyzer and improving the reliability of the POCT blood cell analyzer.

In one embodiment, the pressure building mechanism 12 is used to perform pressure detection on the detection pool during the detection process to obtain the real-time pressure value of the detection pool, and the processor 14 is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result between the real-time pressure value and a first set threshold.

Specifically, in this embodiment, the POCT blood cell analyzer can perform real-time pressure detection on the test pool through the pressure building mechanism 12 during the detection process of the detection unit 13 to obtain the real-time pressure value of the test pool. The pressure building mechanism 12 can continuously perform pressure detection on the test pool during the second detection period to obtain the real-time pressure value. For example, the pressure building mechanism 12 is used to perform pressure detection on the test pool at the beginning of the second detection period to obtain a first real-time pressure value. The processor 14 is used to compare the first real-time pressure value with a first set threshold, and based on the comparison relationship between the first real-time pressure value and the first set threshold, to determine whether the airtightness of the test pool is abnormal. If abnormal, it is determined that the test pool is leaking, and the processor 14 can control the disconnection of the pressure building mechanism 12 from the reagent kit 110 and control the carrier mechanism 11 to unload the reagent kit 110. If the airtightness of the test pool is determined to be normal, the processor 14 continues to control the detection unit 13 to detect the sample in the test pool. The processor 14 is also used to control the pressure building mechanism 12 to continue performing pressure detection on the test pool after a preset time interval to obtain a second real-time pressure value, and the processor 14 is also used to continue to judge the second real-time pressure value. The real-time detection steps of the aforementioned pressure-building mechanism 12 will not end until an abnormality in the airtightness of the detection pool is detected, or until the second detection period ends.

Understandably, the process of the pressure building mechanism 12 detecting the real-time pressure of the detection pool is carried out throughout the second detection period. The processor 14 can determine that the airtightness of the detection pool is abnormal when a real-time pressure value detected in a certain instance is greater than the first set threshold. Alternatively, the processor 14 can determine that the airtightness of the detection pool is abnormal when the real-time pressure value detected in two or more consecutive instances is greater than the first set threshold. Or, the processor 14 can determine that the airtightness of the detection pool is abnormal when the average or standard value of the real-time pressure values in two or more consecutive instances is greater than the first set threshold. No specific limitation is made here.

In this embodiment of the application, the POCT blood cell analyzer uses the pressure building mechanism 12 to detect the pressure of the detection cell during the detection process of the reagent kit 110, so as to obtain the real-time pressure value of the detection cell. The processor 14 is used to determine whether the airtightness of the detection cell is abnormal based on the comparison result of the real-time pressure value and the first set threshold. This allows the reagent kit 110 to be dealt with in a timely manner when the airtightness of the detection cell is abnormal, thereby reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Optionally, the processor 14 is used to generate a first warning message when the real-time pressure value is greater than a first set threshold, so as to indicate that the reagent kit 110 is leaking air through the first warning message.

Specifically, after acquiring the real-time pressure value, the processor 14 compares the real-time pressure value with a first set threshold. If the real-time pressure value exceeds the first set threshold, a first warning message is generated. This first warning message indicates that the reagent kit 110 is leaking. Depending on the connection position between the pressure-building mechanism 12 and the detection cell, a leak in the reagent kit 110 may include leakage in the fore-cell or the rear-cell.

Please refer to Figures 2 and 3. Figure 2 is an operational schematic diagram of an embodiment of the pressure change curve during the normal detection process provided in this application, and Figure 3 is an operational schematic diagram of an embodiment of the pressure change curve during air leakage provided in this application. Since the pressure values in the sample flow from the front chamber to the rear chamber through the micropores have similar trends, exemplarily, since the micropore diameter is fixed and the initial preset pressure during detection is fixed, if there are no external factors (air leakage, pore blockage, etc.) affecting the normal detection process, the real-time pressure change curve detected by the pressure-building mechanism 12 during sample detection is shown in Figure 2. However, when air leakage occurs in the reagent kit 110, the leakage will cause the gas in the fixed space to overflow more rapidly, resulting in a more drastic change in the real-time pressure value detected by the pressure-building mechanism 12 during sample detection, as shown in Figure 3. It can be understood that, as can be seen from the changes in the vertical axis scale of Figures 2 and 3, the slope k1 of the pressure change curve during the normal detection process in Figure 2 is less than the slope k2 of the pressure change curve during air leakage in Figure 3. That is, within the same unit time, the pressure change in the detection chamber during air leakage is greater than the pressure change during the normal detection process.

As shown in Figures 2 and 3, the first set threshold is typically related to the severity of the leak and the initial preset pressure. In one embodiment, depending on the degree of leak, the first set threshold fluctuates based on the real-time pressure value of the pressure change curve during the normal detection process at the same detection time. For example, the first set threshold can be set to a value greater than the real-time pressure value at the same detection time. In another embodiment, the change in the real-time pressure value of the reagent kit 110 can be tested. For example, when the pressure building mechanism 12 is connected to the rear chamber for pressure building, multiple experiments are conducted on the pressure change value of the detection chamber during the sample detection process to obtain multiple experimental schemes as shown in Figure 2. When it is found that the maximum pressure of the detection chamber during normal sample detection does not exceed -27 kPa, the first set threshold can be set to -27 kPa so that the processor 14 can quickly determine that the reagent kit 110 has leaked when the real-time pressure value is greater than the first set threshold and generate a first warning message. For example, when the real-time pressure value is -26 kPa, the real-time pressure value is greater than the first set threshold, and the processor generates a first warning message.

In this embodiment of the application, by continuously monitoring the pressure of the detection pool during the sample detection process to obtain the real-time pressure value of the detection pool, the processor 14 generates a first warning message when the real-time pressure value is greater than a first set threshold, so that the reagent kit 110 can be dealt with in a timely manner when the airtightness of the detection pool is abnormal, thereby reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Furthermore, when the pressure-building mechanism is connected to the rear chamber of the detection pool, the gas volume in the rear chamber decreases due to the sample flowing from the front chamber to the rear chamber, causing the pressure in the rear chamber to gradually rise from a preset negative pressure. The processor generates a first warning message when the real-time pressure value exceeds a first set threshold; that is, the processor performs airtightness judgment in the above manner. When the pressure-building mechanism is connected to the front chamber of the detection pool, the gas volume in the front chamber increases due to the sample flowing from the front chamber to the rear chamber, causing the pressure in the front chamber to gradually decrease from a preset positive pressure. The processor generates a first warning message when the real-time pressure value is less than a first set threshold. The specific steps are similar to those described above and will not be repeated here.

In one embodiment, the pressure building mechanism 12 is used to perform pressure detection on the detection pool at preset time intervals during the detection process to obtain the pressure change value of the detection pool, and the processor 14 is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the pressure change value and a first threshold range.

Specifically, in this embodiment, the POCT blood cell analyzer can perform multiple pressure tests on the detection pool through the pressure building mechanism 12 during the detection process of the detection mechanism 13, with a preset time interval between two adjacent pressure tests, to obtain the pressure change value of the detection pool during two adjacent pressure tests. The processor 14 is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the pressure change value and a first threshold range. The first threshold range is a threshold interval composed of two set thresholds. The comparison result of the pressure change value and the first threshold range is used to indicate whether the pressure change value is within the first threshold range, or, alternatively, to indicate the relationship between the pressure change value and a set threshold within the first threshold range.

Please refer to Figure 4, which is an operational schematic diagram of an embodiment of the pressure change curve during pore blockage provided in this application. Understandably, as shown in Figure 2, during normal detection, since the diameter of the micropore is fixed, the pressure change value of the detection cell is relatively stable when the sample in the front chamber flows through the micropore to the rear chamber. As shown in Figure 3, when the reagent kit 110 leaks, the leak causes a larger change in air pressure within the front or rear chamber, resulting in a larger pressure change value in the detection cell compared to the pressure change value during normal detection. Therefore, the processor 14 can determine whether the reagent kit 110 is leaking based on the comparison result of the pressure change value with a first threshold range. As shown in Figure 4, when the reagent kit 110 is blocked, the blockage makes it difficult for the sample in the front chamber to flow through the micropore to the rear chamber, slowing down the sample flow rate. This results in a smaller pressure change value in the detection cell compared to the pressure change value during normal detection. Therefore, the processor 14 can determine whether the reagent kit 110 is blocked based on the comparison result of the pressure change value with a first threshold range. Understandably, as can be seen from the changes in the vertical axis scale of Figure 2-4, the slope k1 of the pressure change curve during the normal detection process in Figure 2 is greater than the slope k3 of the pressure change curve during the plugging process in Figure 4. That is, k2>k1>k3. Within the same unit time, the pressure change in the detection cell during plugging is less than the pressure change during the normal detection process.

In this embodiment, the POCT blood cell analyzer uses a pressure-building mechanism 12 to detect the pressure in the detection cell during the detection process of the reagent kit 110, thereby obtaining the pressure change value of the detection cell at preset intervals. The processor 14 is used to determine whether the airtightness of the detection cell is abnormal based on the comparison result of the pressure change value and a first threshold range. This allows the reagent kit 110 to be dealt with in a timely manner when the airtightness of the detection cell is abnormal, reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Furthermore, as shown in Figure 2-4, Figure 2-4 is only a pressure change curve of the reagent kit 110 during sample testing in a certain state (e.g., normal testing state, leaking state, or blocked state). It can be understood that when the airtightness of the reagent kit 110 changes, the change process can occur during the sample testing process. For example, there may be sudden air leakage or blocked state during the sample testing process. That is, the pressure change curve obtained by the pressure building mechanism 12 to perform pressure testing on the detection cell may have two or more segments with different slopes. Here, no specific limitation is made on the pressure change curve of the reagent kit 110.

Optionally, the first threshold range includes a second set threshold and a third set threshold, wherein the third set threshold is greater than the second set threshold. The processor 14 is used to generate a first warning message when the pressure change value is greater than the third set threshold, so as to indicate that the reagent kit 110 is leaking air through the first warning message, and/or, the processor 14 is used to generate a second warning message when the pressure change value is less than the second set threshold, so as to indicate that the reagent kit 110 is clogged through the second warning message.

Specifically, the two endpoints of the first threshold range are the second set threshold and the third set threshold, respectively. The first threshold range is a set of values between the second set threshold and the third set threshold. After acquiring the pressure change value, the processor 14 determines whether the pressure change value is within the first threshold range. When the pressure change value is greater than the third set threshold, the pressure change value of the detection cell is larger than the normal detection process, and the processor 14 generates a first warning message to indicate that the reagent kit 110 is leaking. When the pressure change value is less than the second set threshold, the pressure change value of the detection cell is smaller than the normal detection process, and the processor 14 generates a second warning message to indicate that the reagent kit 110 is clogged.

The second and third set thresholds can be related to at least one factor, such as the size of the preset time interval, the size of the micropore diameter, and the size of the initial preset pressure. The second and third set thresholds can be calculated through multiple experiments or by conversion with the pressure change value during normal detection. No specific limitation is made here.

In this embodiment, the processor 14 is used to determine the relationship between the pressure change value and the first threshold range, and generates a first warning message when the pressure change value is greater than a third set threshold, and generates a second warning message when the pressure change value is less than a second set threshold. This enables the reagent kit 110 to be processed according to different abnormal causes when the airtightness of the detection cell is abnormal, thereby reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Optionally, in one embodiment, the pressure building mechanism 12 is used to perform pressure detection on the detection pool during the detection process to obtain a first pressure value. The pressure building mechanism 12 is also used to continue to perform pressure detection on the detection pool after a preset time interval to obtain a second pressure value. The processor 14 is used to calculate the difference between the second pressure value and the first pressure value to obtain a pressure change value.

Specifically, the pressure change value is the difference between the second pressure value obtained at a preset time interval and the first pressure value. When the second detection duration is between 0 and 10 seconds, the preset time interval can be between 0.1 seconds and 1 second. For example, at the initial time (0s) of the second detection duration, the pressure building mechanism 12 performs a first pressure test on the detection pool to obtain a first pressure value. After a preset time interval of 0.1s to 1s, the pressure building mechanism 12 performs a second pressure test on the detection pool to obtain a second pressure value. The processor 14 calculates the difference between the second pressure value and the first pressure value to obtain a first pressure change value. The processor 14 determines whether the airtightness is abnormal based on the first pressure change value. When the first pressure change value is within the first threshold range, the processor 14 continues to control the pressure building mechanism 12 to perform a third pressure test on the detection pool after a preset time interval of 0.1s to 1s to obtain a third pressure value. The processor 14 calculates the difference between the third pressure value and the second pressure value to obtain a second pressure change value. The processor 14 continues to judge the second pressure change until it is determined that the airtightness of the detection pool is abnormal, or until the second detection duration ends.

Furthermore, when the pressure-building mechanism is connected to the rear chamber of the detection pool, the processor performs airtightness judgment in the manner described above. When the pressure-building mechanism is connected to the front chamber of the detection pool, the pressure in the front chamber will gradually decrease from the preset positive pressure during the detection process. The pressure change value mentioned above can be the absolute value of the difference between the second pressure value and the first pressure value, which will not be elaborated further here.

Alternatively, in another embodiment, the pressure change value is the difference between the pressure value of the detection cell during the detection process and the initial pressure value of the detection cell at the start of the test.

Specifically, when the testing mechanism 13 begins testing the sample in the testing pool, the pressure building mechanism 12 performs pressure testing on the testing pool to obtain an initial pressure value. During the second testing period after the start of the test, the pressure building mechanism 12 continues to perform pressure testing on the testing pool at least once to obtain a fourth pressure value. The pressure change value is the difference between the fourth pressure value and the initial pressure value. For example, at 0s of the first testing period, the pressure building mechanism 12 performs pressure testing on the testing pool to obtain an initial pressure value. During the second testing period after 0s (e.g., within 0~10s), the pressure building mechanism 12 can perform pressure testing on the testing pool at preset intervals. For example, the pressure building mechanism 12 can perform pressure testing on the testing pool every 0.1s~1s to obtain a fourth pressure value. The processor 14 is used to calculate the difference between the fourth pressure value and the initial pressure value after each pressure test to obtain a pressure change value, and to determine whether the airtightness of the testing pool is abnormal based on the comparison result of the pressure change value and a first threshold range.

In one embodiment, the pressure building mechanism 12 is used to perform pressure detection on the detection pool within a preset time period of the detection process to obtain the rate of change of the pressure value of the detection pool within the preset time period, and the processor 14 is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the rate of change and a second threshold range.

Specifically, in this embodiment, the POCT blood cell analyzer can perform at least two pressure tests on the detection pool through the pressure building mechanism 12 within a preset time period during the detection process of the detection mechanism 13, so as to obtain the rate of change of the pressure value of the detection pool within the preset time period. The rate of change is used to measure the degree of change of the pressure value of the detection pool relative to time. The preset time period can be a certain time period of the second detection duration. For example, when the second detection duration is 0~10s, the preset time period can be a time interval composed of the first time point and the second time point as the two endpoints, and the difference between the second time point and the first time point is the aforementioned preset time (0.1s~1s). The second detection duration can include multiple preset time periods or can be composed of multiple preset time periods spliced together. The processor 14 is used to perform airtightness judgment based on the pressure value change rate within each preset time period until an airtightness abnormality is determined or the second detection duration ends.

For example, when the second detection duration includes at least two first preset time periods and a second preset time period, the first preset time period can be between 0 and 1 second, and the second preset time period can be between 1 and 2 seconds. The pressure building mechanism 12 is used to perform pressure detection on the detection pool at 0 seconds to obtain a fifth pressure value, and at 1 second to obtain a sixth pressure value. The processor 14 is used to calculate the difference between the sixth pressure value and the fifth pressure value, and to calculate the ratio of the difference to the preset time period to obtain a first rate of change. The processor 14 is used to determine whether the airtightness is abnormal based on the comparison result of the first rate of change and a second threshold range. When the airtightness is normal, the pressure building mechanism 12 is also used to perform pressure detection on the detection pool at 2 seconds to obtain a seventh pressure value. The processor 14 is used to calculate the difference between the seventh pressure value and the sixth pressure value, and to calculate the ratio of the difference to the preset time period to obtain a second rate of change. Alternatively, the processor 14 is used to calculate the difference between the seventh pressure value and the fifth pressure value, and to calculate the ratio of the difference to the preset time period to obtain a second rate of change, until the airtightness is determined to be abnormal or the second detection duration ends.

In this embodiment, the POCT hematology analyzer uses a pressure-building mechanism 12 to detect the pressure in the detection cell during the detection process of the reagent kit 110, thereby obtaining the rate of change of the pressure value in the detection cell within a preset time period. The processor 14 is used to determine whether the airtightness of the detection cell is abnormal based on the comparison result of the rate of change and a second threshold range. This allows the reagent kit 110 to be processed in a timely manner when the airtightness of the detection cell is abnormal, reducing the damage to the POCT hematology analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT hematology analyzer.

Optionally, the second threshold range includes a fourth set threshold and a fifth set threshold, wherein the fifth set threshold is greater than the fourth set threshold. The processor 14 is configured to generate a first warning message when the rate of change is greater than the fifth set threshold, to indicate a leak in the reagent kit 110, and/or, the processor 14 is configured to generate a second warning message when the rate of change is less than the fourth set threshold, to indicate a blockage in the reagent kit 110.

Specifically, the two endpoints of the second threshold range are the fourth set threshold and the fifth set threshold, respectively. The second threshold range is a set of values between the fourth set threshold and the fifth set threshold. After obtaining the rate of change, the processor 14 determines whether the rate of change is within the second threshold range. As shown in Figure 2-4, the rate of change can be correlated with the slope of the change curve in the figure. When the rate of change is greater than the fifth set threshold, the rate of change of the detection cell is larger than that of the normal detection process, and the processor 14 generates a first warning message to indicate that the reagent kit 110 is leaking. When the pressure change value is less than the fourth set threshold, the rate of change of the detection cell is smaller than that of the normal detection process, and the processor 14 generates a second warning message to indicate that the reagent kit 110 is clogged.

The fourth and fifth set thresholds can be related to at least one factor, such as the length of the preset detection time period (i.e., the detection time difference), the size of the micropore diameter, and the magnitude of the initial preset pressure. The fourth and fifth set thresholds can be calculated through multiple experiments or through conversion with the rate of change during normal detection. No specific limitations are made here.

In this embodiment, the processor 14 is used to determine the relationship between the rate of change and the second threshold range, and generates a first warning message when the rate of change is greater than a fifth set threshold, and generates a second warning message when the pressure change value is less than a fourth set threshold. This enables the reagent kit 110 to be processed according to different abnormal causes when the airtightness of the detection cell is abnormal, thereby reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Furthermore, when the pressure-building mechanism is connected to the rear chamber of the detection pool, the slope k3 of the pressure change curve during plugging is less than the pressure change curve k1 during normal detection, and k1 is less than the slope k2 of the pressure change curve during leakage. The processor performs airtightness judgment in the above manner. When the pressure-building mechanism is connected to the front chamber of the detection pool, the absolute value of the slope k3 of the pressure change curve during plugging is less than the absolute value of the pressure change curve k1 during normal detection, and the absolute value of k1 is less than the absolute value of the slope k2 of the pressure change curve during leakage. Therefore, when the pressure-building mechanism is connected to the front chamber of the detection pool, the processor performs airtightness judgment based on the comparison result of the absolute value of the rate of change and the second threshold range, which will not be elaborated further here.

In one embodiment, please refer to Figure 5, which is a structural schematic diagram of the pressure building mechanism in Figure 1. As shown in Figure 5, the pressure building mechanism 12 includes a pressure control component, a pressure sensor 121, and a connector 122. The connector 122 is disposed on the support mechanism 11. The pressure control component is connected to the connector 122 and the pressure sensor 121 respectively through pipelines. The connector 122 is used to dock with the reagent kit 110 so that the pressure control component provides a preset pressure to the detection cell. The pressure sensor 121 is used to detect the pressure of the detection cell.

Specifically, pressure sensor 121 is connected to the pressure control component. When connector 122 is mated with reagent kit 110 and the tubing between connector 122 and pressure control component is open, the pressure detection component and the detection cell are under the same pressure environment, allowing pressure sensor 121 to obtain the pressure value of the detection cell by detecting the pressure value of the pressure control component. In a possible implementation, connector 122 is used to connect to the front chamber of the detection cell, the pressure control component is used to provide a preset positive pressure to the front chamber, and pressure sensor 121 is used to detect the pressure value of the front chamber; alternatively, connector 122 is used to connect to the rear chamber of the detection cell, the pressure control component is used to provide a preset negative pressure to the rear chamber, and pressure sensor 121 is used to detect the pressure value of the rear chamber.

In this process, when the pressure-building mechanism 12 provides a preset pressure to the detection pool, the processor 14 controls the pressure control component to perform a pressure-building operation to establish positive or negative pressure. Simultaneously, the processor 14 controls the pressure sensor 121 to detect the pressure of the pressure control component during the pressure-building process. When the pressure value transmitted by the pressure sensor 121 reaches the preset pressure, the processor 14 controls the pressure control component to stop the pressure-building operation and controls the connection between the pressure control component and the connector 122 to maintain the connection, enabling the detection mechanism 13 to perform impedance counting on the sample in the detection pool under the preset pressure. During the sample detection process of the detection mechanism 13, the pressure sensor 121 also continuously monitors the pressure of the detection pool, allowing the processor 14 to determine whether the airtightness is abnormal based on the pressure detection results.

In this way, the POCT blood cell analyzer of this embodiment can realize pressure control before detection and pressure detection and airtightness judgment during the detection process through pressure sensor 121. By reusing pressure sensor 121, the problem of instrument redundancy caused by new structures is reduced, thereby improving the simplification of POCT blood cell analyzer and reducing the size of POCT blood cell analyzer.

Optionally, the pressure control assembly includes a pressure-building element 123, a negative pressure element 124, a first control element 125, and a second control element 126. The first end of the first control element 125 is connected to the connector 122, the second end of the first control element 125 is connected to the first end of the negative pressure element 124, the first end of the second control element 126 is connected to the second end of the negative pressure element 124, and the second end of the second control element 126 is connected to the pressure-building element 123. The pressure-building element 123 is used to establish negative pressure on the negative pressure element 124 to provide a preset pressure to the detection pool when the first control element 125 is turned on.

Specifically, the pressure-building component 123 includes, but is not limited to, a syringe or other device capable of pressure-building operations. The pressure-building component 123 is used to build pressure on the negative pressure component 124, which stores negative pressure. After the connector 122 is connected to the rear chamber of the detection cell, the processor 14 controls the second control component 126 to conduct, controlling the pressure-building component 123 to perform the pressure-building operation. The processor 14 also detects the pressure of the negative pressure component 124 via the pressure sensor 121, so that when a certain negative pressure is stored in the negative pressure component 124, the second control component 126 is cut off, and the pressure-building component 123 stops working. After the pressure-building operation is completed, the processor 14 controls the first control component 125 to conduct, so that the negative pressure component 124 is connected to the rear chamber, and the rear chamber is under a preset pressure environment. The sample in the front chamber flows to the rear chamber through micropores under the negative pressure of the rear chamber, allowing the detection mechanism 13 to perform impedance counting of the sample during the sample flow.

In this embodiment of the application, the processor 14 can switch the state of the first control element 125 and the second control element 126 so that the pressure building mechanism 12 can be reused to establish the preset negative pressure of the rear pool and to determine the airtightness of the rear pool. This reduces the problem of instrument redundancy caused by the addition of new structures, improves the simplification of the POCT blood cell analyzer and reduces the size of the POCT blood cell analyzer.

In one embodiment, the processor 14 is configured to control the pressure building mechanism 12 to disconnect from the reagent kit 110 when the airtightness of the detection cell is determined to be abnormal.

Specifically, when the processor 14 determines that the airtightness of the test cell is abnormal through the pressure detection result, the processor 14 can control the first control valve to shut off and control the carrier mechanism 11 to unload the reagent kit 110, thereby disconnecting the connection between the connector 122 and the reagent kit 110; or, the processor 14 can also control the carrier mechanism 11 to directly unload the reagent kit 110, thereby disconnecting the pressure building mechanism 12 from the reagent kit 110.

In this embodiment of the application, when the processor 14 determines that the airtightness of the test cell is abnormal, it controls the pressure building mechanism 12 to disconnect from the reagent kit 110. This can prevent the sample in the test cell from entering the pipeline through the connector 122 when the airtightness is abnormal, thereby reducing the contamination of the connector 122 and the pipeline caused by sample backflow and improving the accuracy and reliability of the POCT blood cell analyzer.

In one embodiment, the detection cell includes a front cell, a rear cell, and a microporous sheet. The microporous sheet has micropores. The front cell communicates with the rear cell through the micropores in the microporous sheet. The detection mechanism 13 is used to count cells in the sample as it flows from the front cell to the rear cell through the microporous sheet. The processor 14 is used to acquire the flow rate of the sample as it passes through the microporous sheet, and to determine whether the airtightness of the detection cell is abnormal based on the flow rate change during the detection process. Alternatively, the processor 14 is also used to calculate the flow pressure drop of the sample based on the flow rate, and to determine whether the airtightness of the detection cell is abnormal based on the flow pressure drop during the detection process.

Specifically, the processor 14 can detect the flow rate of the sample passing through the microporous plate using devices such as a flow meter, velocity meter, and water flow sensor to obtain the flow rate of the sample passing through the microporous plate. In one embodiment, since the sample flow in the detection cell is driven by pressure, the pressure in the detection cell will change with a similar trend as the sample flows from the front cell to the rear cell, causing the flow rate of the sample to gradually decrease with a similar trend. Therefore, this embodiment can determine whether the airtightness of the detection cell is abnormal by the change in flow rate. For example, if the flow rate is significantly reduced compared to the normal testing process, it is determined that the reagent kit 110 is blocked; or, if the flow rate is slightly reduced compared to the normal testing process, it is determined that the reagent kit 110 is leaking. The change in flow rate can be obtained by flow rate testing at multiple time points, and is not specifically limited here. The processor 14 can also determine the airtightness by the change in flow rate during the detection process. Since flow rate is related to flow velocity, it will not be elaborated here.

In another embodiment, the process of calculating the flow pressure drop of the sample based on the flow velocity described above can be calculated using the relevant principles of pressure loss in fluid dynamics, and will not be elaborated further here. When the flow velocity of the sample changes, as the sample flows from the side of the microporous sheet closer to the forecell to the side closer to the rearcell, the flow pressure drop of the sample will also change similarly. Therefore, this embodiment can also determine whether the airtightness of the detection cell is abnormal by observing the change in flow pressure drop during the detection process.

In this embodiment, the processor 14 can also detect the flow rate of the sample as it passes through the microporous sheet to determine whether the airtightness of the detection cell is abnormal based on the flow rate change during the detection process. Alternatively, the processor 14 can also calculate the flow pressure drop of the sample based on the flow rate and determine whether the airtightness of the detection cell is abnormal based on the flow pressure drop during the detection process. This allows the reagent kit 110 to be processed in a timely manner when the airtightness of the detection cell is abnormal, reducing the damage to the POCT blood cell analyzer caused by problems such as air leakage or pore blockage, and improving the detection accuracy and reliability of the POCT blood cell analyzer.

Optionally, the processor 14 can combine at least one of the above-mentioned flow rate, flow volume, or flow pressure drop to the pressure detection result to determine air tightness. The processor 14 can also determine air tightness by using at least one of the flow rate, flow volume, or flow pressure drop alone, without specific limitations.

In one embodiment, the detection mechanism 13 is used to perform impedance counting on the sample flowing through the microporous sheet from the front chamber to the rear chamber. In one embodiment, the detection waste liquid after impedance counting is completed is stored in the rear chamber. In another embodiment, the detection chamber further includes a waste liquid tank, which is connected to the rear chamber, and a pressure building mechanism 12 is connected to the waste liquid tank to provide a preset pressure to the waste liquid tank. Under the preset pressure of the waste liquid tank, the sample from the front chamber flows through the microporous sheet to the rear chamber, and the detection waste liquid from the rear chamber continues to be driven to the waste liquid tank under pressure, so that the detection waste liquid is stored in the waste liquid tank.

Please refer to Figure 6, which is a schematic flowchart of an embodiment of the blood cell analysis method provided in this application. As shown in Figure 6, the blood cell analysis method of this embodiment includes the following steps:

Step S11: Receive kit 110, the detection cell of kit 110 stores the sample to be tested.

After receiving the reagent kit 110, the reagent kit 110 is electrically connected to the detection mechanism 13, and the reagent kit 110 is also connected to the connector 122 of the pressure building mechanism 12.

Step S12: Perform impedance detection on the sample from kit 110.

The control and detection mechanism 13 performs impedance testing on the sample in the detection cell so that the sample stored in the front cell flows through the micropore to the rear cell, and obtains the detection result of the sample by acquiring the change of electrical signal when the sample passes through the micropore.

Step S13: During the impedance detection process, pressure is tested on the detection cell to determine whether the airtightness of the detection cell is abnormal based on the pressure test results of the pressure building mechanism 12.

Specifically, during the impedance detection process, pressure testing is performed on the detection cell. The duration of pressure testing is within the duration of impedance detection, and the end time of pressure testing is before the end time of impedance detection. Based on the pressure test results of the pressure building mechanism 12, it is determined whether the airtightness of the detection cell is abnormal. If the airtightness of the detection cell is determined to be abnormal, the reagent kit 110 can be dealt with in a timely manner, reducing the damage to the POCT hematology analyzer caused by problems such as air leakage or blockage of the reagent kit 110, and improving the detection accuracy and reliability of the POCT hematology analyzer.

Example 1:

The pressure-building mechanism 12 is used to build up pressure in the reagent kit 110 so that the detection cell of the reagent kit 110 is at a preset pressure of -29 kPa to -31 kPa. The detection mechanism 13 detects the sample in the detection cell. The pressure-building mechanism 12 is also used to continuously detect the pressure of the detection cell within 10 seconds during the detection process to obtain a real-time pressure value. The processor 14 is used to generate a first warning message when the real-time pressure value is greater than -27 kPa.

Example 2:

The pressure-building mechanism 12 is used to build up pressure in the reagent kit 110 so that the detection cell of the reagent kit 110 is at a preset pressure of -29 kPa to -31 kPa. The detection mechanism 13 detects the sample in the detection cell. The pressure-building mechanism 12 is also used to detect the pressure in the detection cell at preset intervals (0.1 s to 1 s) within 10 s of the detection process to obtain the pressure change value. The processor 14 is used to generate a first warning message when the pressure change value is greater than 3 kPa; the processor 14 is also used to generate a second warning message when the pressure change value is less than 0.1 kPa.

Example 3:

The pressure-building mechanism 12 is used to build up pressure in the reagent kit 110 so that the detection cell of the reagent kit 110 is at a preset pressure of -29 kPa to -31 kPa. The detection mechanism 13 detects the sample in the detection cell. The pressure-building mechanism 12 is also used to perform pressure detection on the detection cell for a preset time period within 10 seconds of the detection process to obtain the rate of change of the pressure value. The processor 14 is used to generate a first warning message when the rate of change is greater than 0.3; the processor 14 is also used to generate a second warning message when the rate of change is less than 0.01.

Any process or method described in the flowchart or otherwise herein can be understood as representing a module, segment, or portion of code comprising one or more executable instructions for implementing a particular logical function or process, and the scope of the preferred embodiments of this application includes additional implementations in which functions may be performed not in the order shown or discussed, including substantially simultaneously or in reverse order depending on the function involved, as will be understood by those skilled in the art to which embodiments of this application pertain.

The logic and/or steps represented in the flowchart or otherwise described herein, for example, can be considered as a sequenced list of executable instructions for implementing logical functions, and can be embodied in any computer-readable medium for use by, or in conjunction with, an instruction execution system, apparatus or device (which may be a personal computer, server, network device or other system that can fetch and execute instructions from, an instruction execution system, apparatus or device).

The above description is merely an embodiment of this application and does not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims (15)

A POCT hematology analyzer, comprising: A carrier mechanism for loading a reagent kit, wherein the detection cell of the reagent kit stores the sample to be tested; A pressure-building mechanism is connected to the reagent kit on the support mechanism, and the pressure-building mechanism is used to provide a preset pressure to the detection cell; A testing mechanism for testing samples in the testing pool under the preset pressure; The processor is connected to both the pressure-building mechanism and the detection mechanism. The pressure-building mechanism is also used to perform pressure detection on the detection pool during the detection process of the detection mechanism, and the processor is used to determine whether the airtightness of the detection pool is abnormal based on the pressure detection result of the pressure-building mechanism. According to claim 1, the POCT blood cell analyzer is wherein the detection mechanism is used to detect the sample in the detection pool under the preset pressure within a first detection time, the pressure building mechanism performs pressure detection on the detection pool for a second detection time, the second detection time is within the first detection time, and the end time of the second detection time is before the end time of the first detection time. According to claim 1, the POCT blood cell analyzer is wherein the pressure building mechanism is used to perform pressure detection on the detection pool during the detection process to obtain the real-time pressure value of the detection pool, and the processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the real-time pressure value and a first set threshold. According to claim 3, the POCT blood cell analyzer is configured to generate a first warning message when the real-time pressure value is greater than the first set threshold, so as to indicate that the reagent kit is leaking air through the first warning message. According to claim 1, the POCT blood cell analyzer is wherein the pressure building mechanism is used to perform pressure detection on the detection pool at preset time intervals during the detection process to obtain the pressure change value of the detection pool, and the processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the pressure change value and a first threshold range. According to claim 5, the POCT blood cell analyzer, wherein the first threshold range includes a second set threshold and a third set threshold, and the third set threshold is greater than the second set threshold; The processor is configured to generate a first warning message when the pressure change value is greater than the third preset threshold, so as to indicate that the reagent kit is leaking air, and/or, the processor is configured to generate a second warning message when the pressure change value is less than the second preset threshold, so as to indicate that the reagent kit is clogged. According to claim 5, the POCT blood cell analyzer is wherein the pressure building mechanism is used to perform pressure detection on the test pool during the detection process to obtain a first pressure value, the pressure building mechanism is further used to continue to perform pressure detection on the test pool after a preset time interval to obtain a second pressure value, and the processor is used to calculate the difference between the second pressure value and the first pressure value to obtain the pressure change value. According to claim 5, the POCT blood cell analyzer is wherein the pressure change value is the difference between the pressure value of the detection cell during the detection process and the initial pressure value of the detection cell at the start of the test. According to claim 1, the POCT blood cell analyzer is wherein the pressure building mechanism is used to perform pressure detection on the detection pool within a preset time period of the detection process to obtain the rate of change of the pressure value of the detection pool within the preset time period, and the processor is used to determine whether the airtightness of the detection pool is abnormal based on the comparison result of the rate of change and a second threshold range. According to claim 9, the POCT blood cell analyzer, wherein the second threshold range includes a fourth set threshold and a fifth set threshold, and the fifth set threshold is greater than the fourth set threshold; The processor is configured to generate a first warning message when the rate of change is greater than the fifth preset threshold, so as to indicate that the reagent kit is leaking air, and/or, the processor is configured to generate a second warning message when the rate of change is less than the fourth preset threshold, so as to indicate that the reagent kit is clogged. According to claim 1, the POCT blood cell analyzer includes a pressure control component, a pressure sensor, and a connector. The connector is disposed on the support mechanism. The pressure control component is connected to the connector and the pressure sensor via pipelines. The connector is used to dock with the reagent kit so that the pressure control component provides a preset pressure to the detection cell. The pressure sensor is used to detect the pressure of the detection cell. According to claim 11, the POCT blood cell analyzer, wherein the pressure control component includes a pressure building element, a negative pressure element, a first control element, and a second control element, wherein a first end of the first control element is connected to the connector, a second end of the first control element is connected to a first end of the negative pressure element, a first end of the second control element is connected to a second end of the negative pressure element, and a second end of the second control element is connected to the pressure building element, wherein the pressure building element is used to establish negative pressure on the negative pressure element to provide the preset pressure to the detection cell when the first control element is turned on. According to claim 1, the POCT hematology analyzer is wherein the processor is configured to control the pressure building mechanism to disconnect from the reagent kit when the airtightness of the detection cell is determined to be abnormal. According to claim 1, the POCT blood cell analyzer includes a front pool, a rear pool, and a microwell plate. The front pool is connected to the rear pool through the microwell plate. The detection mechanism is used to count the cells in the sample when the sample in the front pool flows to the rear pool through the microwell plate. The processor is used to obtain the flow rate of the sample as it passes through the microporous sheet, so as to determine whether the airtightness of the detection cell is abnormal based on the flow rate change during the detection process. Alternatively, the processor is also used to calculate the flow pressure drop of the sample based on the flow rate, and determine whether the airtightness of the detection cell is abnormal based on the flow pressure drop during the detection process. A blood cell analysis method, comprising: Receive the reagent kit, wherein the detection cell of the reagent kit stores the sample to be tested; Impedance detection was performed on the samples from the kit. During the impedance detection process, the pressure of the detection cell is measured to determine whether the airtightness of the detection cell is abnormal based on the pressure measurement result of the pressure building mechanism.