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WO2025181685A3 - Process for obtaining a soluble single-cell protein (pro60) that can be used in various food matrices - Google Patents

Process for obtaining a soluble single-cell protein (pro60) that can be used in various food matrices

Info

Publication number
WO2025181685A3
WO2025181685A3 PCT/IB2025/052044 IB2025052044W WO2025181685A3 WO 2025181685 A3 WO2025181685 A3 WO 2025181685A3 IB 2025052044 W IB2025052044 W IB 2025052044W WO 2025181685 A3 WO2025181685 A3 WO 2025181685A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell protein
pro60
obtaining
food matrices
various food
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/IB2025/052044
Other languages
Spanish (es)
French (fr)
Other versions
WO2025181685A2 (en
Inventor
Navarro Lizárraga MIGUEL ÁNGEL
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proteo Alimentaria SAPI De CV
Original Assignee
Proteo Alimentaria SAPI De CV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteo Alimentaria SAPI De CV filed Critical Proteo Alimentaria SAPI De CV
Publication of WO2025181685A2 publication Critical patent/WO2025181685A2/en
Publication of WO2025181685A3 publication Critical patent/WO2025181685A3/en
Pending legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/165—Yeast isolates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/18—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/20—Proteins from microorganisms or unicellular algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
    • A23L31/10—Yeasts or derivatives thereof
    • A23L31/15—Extracts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention relates to a novel fermentation, extraction and purification process for obtaining a single-cell protein for use in the food industry, using a particular strain of Kluyveromyces marxianus referred to as KMPROTEO 1, which results in a product consisting of a single-cell protein powder with a protein content equal to or greater than 60%, that is highly bioavailable, which, since it is soluble, can be included in different food matrices, without changing the formulation or the profile of the product to which it is added, which we have named "PRO60". The process disclosed in this specification includes the description of the aerobic fermentation of the strain of Kluyveromyces marxianus referred to as KMPROTEO 1, as well as the use of the ingredient or final product obtained, by adding same to some preferred food matrices.
PCT/IB2025/052044 2024-02-26 2025-02-26 Process for obtaining a soluble single-cell protein (pro60) that can be used in various food matrices Pending WO2025181685A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MX2024002466A MX2024002466A (en) 2024-02-26 2024-02-26 PROCESS FOR OBTAINING A SOLUBLE UNICELLULAR PROTEIN (PRO60) THAT CAN BE USED IN VARIOUS FOOD MATRICES.
MXMX/A/2024/002466 2024-02-26

Publications (2)

Publication Number Publication Date
WO2025181685A2 WO2025181685A2 (en) 2025-09-04
WO2025181685A3 true WO2025181685A3 (en) 2025-10-23

Family

ID=92301623

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2025/052044 Pending WO2025181685A2 (en) 2024-02-26 2025-02-26 Process for obtaining a soluble single-cell protein (pro60) that can be used in various food matrices

Country Status (2)

Country Link
MX (1) MX2024002466A (en)
WO (1) WO2025181685A2 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017036294A1 (en) * 2015-08-28 2017-03-09 复旦大学 Kluyveromyces marxianus and use thereof
US20200123494A1 (en) * 2017-06-16 2020-04-23 Proteo Alimentaria, S.A.P.I. de C.V. Method for obtaining protein from whey or molasses
MX2019004550A (en) * 2019-04-17 2020-10-19 Proteo Alimentaria S A P I De C V A strain of kluyveromyces marxianus and fermentation process for obtaining unicellular biomass, for its application in the industry.
WO2024005625A1 (en) * 2022-06-30 2024-01-04 Proteo Alimentaria, S.A.P.I. de C.V. Production process and formulation of a single-cell protein by fermenting the yeast kluyveromyces marxianus

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3887431A (en) 1972-11-29 1975-06-03 Anheuser Busch Yeast protein isolate with reduced nucleic acid content and process of making same
US3991215A (en) 1974-02-11 1976-11-09 Anheuser-Busch, Incorporated Manufacture of yeast protein isolate having a reduced nucleic acid content by a thermal process
US3947605A (en) 1974-10-30 1976-03-30 Standard Oil Company Process for preparing high yields of single cell products having reduced purine content and high nutritive value
JPS63112965A (en) 1986-06-09 1988-05-18 Takeda Chem Ind Ltd Production of yeast extract
BG48706A1 (en) 1989-01-31 1991-05-15 Vissh Inst Khranitelno Vkusova Method for preparing protein
JP2885935B2 (en) 1990-02-01 1999-04-26 オリエンタル酵母工業株式会社 Production method of yeast extract
US20130122145A1 (en) 2010-05-14 2013-05-16 Lactoscience Inc. Fermentation process of a substrate using a mixed culture for the production of an edible biomass for animal and/or human consumption
CN105707436A (en) 2016-01-28 2016-06-29 复旦大学 Kluyveromyces marxianus yeast application
CN105087403B (en) 2015-08-28 2018-04-03 复旦大学 Kluyveromyces marx and its application
FR3080521B1 (en) 2018-04-27 2021-07-09 Lesaffre & Cie YEAST PROTEINS
EP3670646A1 (en) 2018-12-21 2020-06-24 Ohly GmbH Functional yeast protein concentrate

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017036294A1 (en) * 2015-08-28 2017-03-09 复旦大学 Kluyveromyces marxianus and use thereof
US20200123494A1 (en) * 2017-06-16 2020-04-23 Proteo Alimentaria, S.A.P.I. de C.V. Method for obtaining protein from whey or molasses
MX2019004550A (en) * 2019-04-17 2020-10-19 Proteo Alimentaria S A P I De C V A strain of kluyveromyces marxianus and fermentation process for obtaining unicellular biomass, for its application in the industry.
WO2024005625A1 (en) * 2022-06-30 2024-01-04 Proteo Alimentaria, S.A.P.I. de C.V. Production process and formulation of a single-cell protein by fermenting the yeast kluyveromyces marxianus
EP4483720A1 (en) * 2022-06-30 2025-01-01 Proteo Alimentaria, S.A.P.I de C.V. <smallcaps/>? ? ?kluyveromyces marxianus? ? ? ? ?production process and formulation of a single-cell protein by fermenting the yeast

Also Published As

Publication number Publication date
MX2024002466A (en) 2024-04-26
WO2025181685A2 (en) 2025-09-04

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