WO2025000274A1 - Kit/test paper for detecting ammonia nitrogen, and detection system and related method thereof - Google Patents

Abstract

A kit/test paper for detecting ammonia nitrogen, and a detection system and a related method thereof. A reagent in the kit/test paper comprise the following raw materials in parts by mass: 50-500 parts of a buffering agent, 0-500 parts of a masking agent, 0-500 parts of a stabilizer, 50-500 parts of a color developing agent, 2-100 parts of an oxidizing agent, and 1-100 parts of a catalyst. The specification of the reagent is 0.1 g per tube. The preparation process is simple, no special environmental requirement is required, no special three-waste pollution is caused, the cost is low, the detection steps are simple, the determination is more accurate, the color developing time is short, and the mobility is high. The reagents used are common chemical reagents, are safe and reliable, and have low price. The kit/test paper has a wide application range, and can be used for measuring the content of ammonia nitrogen in water bodies such as chemical wastewater, surface water, urban wastewater recycling-landscape environment water and urban miscellaneous water, especially for environmentally friendly monitoring of ammonia nitrogen.

Description

A kit/test paper for detecting ammonia nitrogen, a detection system and related methods Technical Field

The present invention relates to the field of material technology, and in particular to a kit/test paper for detecting ammonia nitrogen, a detection system and related methods thereof.

Background Art

Ammonia nitrogen refers to nitrogen in the form of free ammonia (NH ₃ ) and ammonium ions (NH ₄ ⁺ ) in water. Ammonia nitrogen is a nutrient in water bodies, which can cause eutrophication of water. It is the main oxygen-consuming pollutant in water bodies and is toxic to fish and some aquatic organisms. Ammonia nitrogen in water can be converted into nitrite under certain conditions. If it is consumed for a long time, the nitrite in the water will combine with protein to form nitrosamine, which is a strong carcinogen and extremely harmful to human health. Ammonia nitrogen, as the main excessive pollutant, appears very frequently in the seven major water systems, and ammonia nitrogen pollution has become a national pollution problem. During the "12th Five-Year Plan" period, ammonia nitrogen was listed as one of the indicators for the total amount of water pollutants. According to the national "Comprehensive Standard for Sewage Discharge", all pollutant discharge units have requirements for the ammonia nitrogen content in sewage discharge, among which the first-level standard for ammonia nitrogen in industrial wastewater is 15mg/L and the second-level standard is 50mg/L.

The main methods for ammonia nitrogen detection include traditional spectrophotometry, electrochemical analysis, ammonia nitrogen detection kits or ammonia nitrogen detection test strips. Traditional spectrophotometry includes Nessler's reagent method, salicylic acid-hypochlorite colorimetry, indigo blue photometry, etc.; electrochemical analysis includes ammonia sensitive electrode, stripping-conductivity method, and ammonium ion selective electrode. However, these existing ammonia nitrogen detection methods still have defects. Spectrophotometry and electrochemical analysis are accurate, but the steps are cumbersome, the detection location is limited, and the instrument is expensive and not easy to carry. Ammonia nitrogen detection kits or ammonia nitrogen detection test strips are not restricted by the site and are easy to carry. Operators can detect samples at any time and place, and the operation is simple and fast. However, the ammonia nitrogen detection kits or ammonia nitrogen detection test strips currently on the market require multi-step operations. For example, an activator is required, but the activator has poor stability, is not easy to store for a long time, and is corrosive to the human body. The detection must first add an activator, and then use the ammonia nitrogen detection kit or ammonia nitrogen detection test strip for detection, which is a cumbersome process. The test results deviate greatly from the actual results. Therefore, it is urgent to improve the determination method of ammonia nitrogen.

Summary of the invention

In order to solve the above technical problems, the present invention provides a kit/test paper for detecting ammonia nitrogen, a detection system and related methods thereof.

To achieve the above purpose, the technical solution adopted by the present invention is as follows:

The first aspect of the present invention provides a kit/test paper for detecting ammonia nitrogen, wherein the reagent in the kit/test paper comprises the following raw materials in parts by mass: 50 to 500 parts of a buffer, 0 to 500 parts of a masking agent, 0 to 500 parts of a stabilizer, 50 to 500 parts of a developer, 2 to 100 parts of an oxidant and 1 to 100 parts of a catalyst; the specification of the reagent is 0.1 g per tube; wherein the buffer is used to keep the pH of the reagent greater than 12 after being dissolved in 2 mL to 10 mL of water; the masking agent is citric acid The oxidant is at least one of sodium hypochlorite, bleaching powder, sodium dichloroisocyanurate and chlorine dioxide; the catalyst is at least one of cyanohydrin, sodium nitrosoferrocyanide, potassium nitrosoferrocyanide and magnesium nitrosoferrocyanide.

Further, the buffer is at least one of disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium hydroxide, potassium hydroxide, borax, sodium carbonate, sodium bicarbonate, boric acid, potassium chloride, borax, sodium phosphate, potassium phosphate, glycine, sodium dimethylaminoacetate or sodium diethylbarbiturate. For water samples of ordinary water quality, the buffer can be selected from conventional buffers. For water samples with higher mineralization, the concentration of the buffer reagent is increased or multiple buffer systems are superimposed, preferably selected from a phosphate buffer salt system or a phosphate buffer salt system superimposed with other buffer systems.

Furthermore, the storage conditions of the test kit/test paper are a cool and dry place with a storage temperature below 26°C. If the temperature is higher than 25°C, the unused test kit/test paper should be stored in the fresh-keeping layer of the refrigerator. It should be stored in a light-proof and moisture-proof package, and the effective period of use is about one year.

The second aspect of the present invention provides a method for preparing a kit/test paper for detecting ammonia nitrogen, the kit/test paper comprising a kit and a test paper;

The preparation method of the kit comprises the following steps: taking raw materials of various components according to the ratio, grinding the raw materials of various components sufficiently until they can pass through an 80-mesh standard sieve and mixing them evenly, and then packaging them into 0.1 g per tube to obtain a reagent; vacuumizing the reagent or sealing it with an inert gas to obtain the kit;

The preparation method of the test paper comprises the following steps: taking raw materials of various components according to a ratio, grinding the raw materials of various components sufficiently until they can all pass through an 80-mesh standard sieve and mixing them uniformly, and then packaging them into 0.1 g per tube to obtain a reagent; dissolving the reagent in 1 mL to 5 mL of ammonia-free water to obtain a reagent solution; immersing all quantitative medium-speed filter paper in the reagent solution, and oscillating on a constant temperature oscillator for 10 min to 120 min; taking out the filter paper, placing it in parallel at room temperature, hanging it in the air to dry, cutting it, and sealing it in plastic for later use to obtain the test paper.

A third aspect of the present invention provides an ammonia nitrogen detection system, which includes the above-mentioned kit/test paper for detecting ammonia nitrogen, and a standard colorimetric card.

The fourth aspect of the present invention provides a method for preparing an ammonia nitrogen detection system, the method comprising: preparing the kit or test paper, and preparing the standard colorimetric card to obtain the system; wherein, in the preparation of the standard colorimetric card, the method comprises: drying ammonium sulfate at a temperature of 110°C for 2h, and then dissolving it in ammonia-free water to prepare concentrations of 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.75mg/L, 1.0mg/L, 1.5mg/L, 2.0mg/L respectively. , 4.0mg/L, 6.0mg/L, 8.0mg/L, 10mg/L, 12mg/L, 14mg/L, 16mg/L, 18mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L, and 1000mg/L ammonia nitrogen standard solution, take 1mL~5mL of various ammonia nitrogen standard solutions, add 0.1g of solid reagent respectively, seal the packages with caps, shake well, and the color is basically stable after 5 minutes of color development to obtain a standard colorimetric card.

A fifth aspect of the present invention provides an application method of an ammonia nitrogen detection system. When the system includes a test kit and a standard colorimetric card, the application method of the system includes: adding 1 mL to 5 mL of a solution to be tested into a tube of the test kit, and comparing the depth of the blue-green color presented by the test kit with the standard colorimetric card to determine the concentration of ammonia nitrogen in the test solution; if ammonia nitrogen is present in the water, a blue-green color is displayed, and the depth of the color is related to the concentration of ammonia nitrogen in the water.

In the case where the system includes a test paper and a standard colorimetric card, the application method of the system includes: dropping a drop of the solution to be tested on the test paper, leaving it to stand for 3 to 5 minutes, and comparing the color displayed on the test paper with the standard colorimetric card to determine the concentration of ammonia nitrogen in the solution to be tested. The color displayed on the test paper deepens as the concentration of ammonia nitrogen increases, and the color changes from light blue to dark blue, which can determine the concentration of ammonia nitrogen in the solution to be tested.

A sixth aspect of the present invention provides the use of the above-mentioned kit/test paper for detecting ammonia nitrogen in the field of water quality detection.

The seventh aspect of the present invention provides the application of the above-mentioned ammonia nitrogen detection system in the field of water quality detection, which can be specifically applied in the field of water quality detection of river water, sewage, surface water, etc.

Compared with the prior art, the technical solution provided by the present invention has at least the following advantages:

The present invention provides a kit/test paper for detecting ammonia nitrogen, a detection system and related methods thereof. The ammonia nitrogen detection kit or ammonia nitrogen detection test paper for determining ammonia nitrogen in water quality provided by the present invention has a simple manufacturing process, no special environmental requirements, no special three wastes pollution, low cost, simple detection steps and more accurate determination, short color development time and strong mobility. In the present invention, the buffer, masking agent, stabilizer, developer, oxidant and catalyst are all loaded in a container or loaded on the test paper. Compared with traditional methods or other determination forms, it is more convenient to use and does not require specialized technicians to perform determinations. The reagents used in the present invention are all common chemical reagents, which are safe, reliable and inexpensive. The test paper has a wide range of applications and can be used to determine the ammonia nitrogen content of water bodies such as chemical wastewater, surface water, urban sewage recycling-landscape environment water and urban miscellaneous water, especially in the monitoring of environmental ammonia nitrogen.

1) Water sample testing can be completed in just a few minutes.

2) The operation is simple. Just add the water sample to be tested into the colorimetric tube (on the test paper) and shake well to get the colorimetric result.

3) Easy to carry, the size of a noodle, can be carried with you.

4) The present invention has strong anti-interference capability.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a standard colorimetric chart (unit in the figure is ppm) in an embodiment of the present invention.

DETAILED DESCRIPTION

The principle of sodium salicylate method for detecting ammonia nitrogen: in the presence of sodium nitroprusside, the ammonia and ammonium ions dispersed in the water react with salicylic acid or sodium salicylate and its hypochlorite ions to transform into dark blue chemical substances. This method has the advantages of being smart and stable, simple in actual operation, and less in laboratory environmental pollution, and is widely used. The inventors found that the currently commercially available ammonia nitrogen detection kits or ammonia nitrogen detection test strips require multi-step operations, for example, an activator is required, but the activator has poor stability, is not easy to store for a long time, and the activator is corrosive to the human body. The detection must first add an activator, and then use an ammonia nitrogen detection kit or ammonia nitrogen detection test strip for detection, and the process is more cumbersome. The test results deviate greatly from the actual results. Therefore, there is an urgent need to improve the determination method of ammonia nitrogen.

The Chinese patent application number 200510037389.8 discloses a rapid detection reagent for ammonia nitrogen concentration in water and its usage. The patent is a two-component package of A and B. When using, add the A reagent package to the water sample, let it stand for 2 minutes, then add the B reagent package. After standing for 10 minutes, it can be compared with the standard colorimetric card to read the ammonia nitrogen concentration in the water. The color development time of the patented determination method is more than 10 minutes, which is a long time. In addition, it is a two-component method, and it is necessary to add component A first, and then add component B after 2 minutes, which is more troublesome to operate.

The Chinese patent application number 201510012427.8 discloses an ammonia nitrogen test strip and its application method. When using, add activator to the water sample to be tested, 4 drops of activator are required for every 1 ml of water sample to be tested, shake well and take a drop of the mixture on the test strip, let it stand for 3 to 5 minutes, and compare it with the standard colorimetric card to determine the concentration of ammonia nitrogen in the sample to be tested.

Chinese patent application number 201310551524.5 discloses a reagent and method for rapidly determining the ammonia nitrogen content in water samples. The color development time of the patented determination method is 5-10 minutes, which is relatively long. In addition, the test paper prepared by the method disclosed in the patent displays yellow color for different nitrogen-containing solutions, and the color distinction is not obvious.

The purpose of the present invention is to overcome the shortcomings and deficiencies of the prior art, and to provide a kit and test paper for rapid detection of ammonia nitrogen in water. The ammonia nitrogen detection kit or ammonia nitrogen detection test paper for determining ammonia nitrogen in water quality provided by the present invention has a simple manufacturing process, no special environmental requirements, no special three wastes pollution, low cost, simple detection steps and more accurate determination, short color development time and strong mobility. In the present invention, the buffer, masking agent, stabilizer, developer, oxidant, and catalyst are all loaded in a container or loaded on the test paper. Compared with traditional methods or other determination forms, it is more convenient to use and does not require specialized technicians to perform determinations. The reagents used in the present invention are all common chemical reagents, safe and reliable, and low in price. The test paper has a wide range of applications and can be used to determine the ammonia nitrogen content of water bodies such as chemical sewage, surface water, urban sewage recycling-landscape environment water and urban miscellaneous water, especially in the monitoring of environmental ammonia nitrogen.

The first aspect of the present invention provides a kit/test paper for detecting ammonia nitrogen, wherein the reagent in the kit/test paper comprises the following raw materials in parts by mass: 50 to 500 parts of a buffer, 0 to 500 parts of a masking agent, 0 to 500 parts of a stabilizer, 50 to 500 parts of a developer, 2 to 100 parts of an oxidant and 1 to 100 parts of a catalyst; the specification of the reagent is 0.1 g per tube; wherein the buffer is used to keep the pH of the reagent greater than 12 after being dissolved in 2 mL to 10 mL of water; the masking agent is citric acid The oxidant is at least one of sodium hypochlorite, bleaching powder, sodium dichloroisocyanurate and chlorine dioxide; the catalyst is at least one of cyanohydrin, sodium nitrosoferrocyanide, potassium nitrosoferrocyanide and magnesium nitrosoferrocyanide.

The second aspect of the present invention provides a method for preparing a kit/test paper for detecting ammonia nitrogen, the kit/test paper comprising a kit and a test paper;

The preparation method of the kit comprises the following steps: taking raw materials of various components according to the ratio, grinding the raw materials of various components sufficiently until they can pass through an 80-mesh standard sieve and mixing them evenly, and then packaging them into 0.1 g per tube to obtain a reagent; vacuumizing the reagent or sealing it with an inert gas to obtain the kit;

The preparation method of the test paper comprises the following steps: taking raw materials of various components according to a ratio, grinding the raw materials of various components sufficiently until they can all pass through an 80-mesh standard sieve and mixing them uniformly, and then packaging them into 0.1 g per tube to obtain a reagent; dissolving the reagent in 1 mL to 5 mL of ammonia-free water to obtain a reagent solution; immersing all quantitative medium-speed filter paper in the reagent solution, and oscillating on a constant temperature oscillator for 10 min to 120 min; taking out the filter paper, placing it in parallel at room temperature, hanging it in the air to dry, cutting it, and sealing it in plastic for later use to obtain the test paper.

A third aspect of the present invention provides an ammonia nitrogen detection system, which includes the above-mentioned kit/test paper for detecting ammonia nitrogen, and a standard colorimetric card.

The fourth aspect of the present invention provides a method for preparing an ammonia nitrogen detection system, the method comprising: preparing the kit or test paper, and preparing the standard colorimetric card to obtain the system; wherein, in the preparation of the standard colorimetric card, the method comprises: drying ammonium sulfate at a temperature of 110°C for 2h, and then dissolving it in ammonia-free water to prepare concentrations of 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.75mg/L, 1.0mg/L, 1.5mg/L, 2.0mg/L, 4 .0mg/L, 6.0mg/L, 8.0mg/L, 10mg/L, 12mg/L, 14mg/L, 16mg/L, 18mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L ammonia nitrogen standard solutions, take 1m~5mL of various ammonia nitrogen standard solutions, add 0.1g of solid reagent respectively, seal the packages with caps, shake well, and the color is basically stable after 5 minutes of color development to obtain a standard colorimetric card, as shown in Figure 1.

A fifth aspect of the present invention provides an application method of an ammonia nitrogen detection system. When the system includes a test kit and a standard colorimetric card, the application method of the system includes: adding 1 mL to 5 mL of a solution to be tested into a tube of the test kit, and comparing the depth of the blue-green color presented by the test kit with the standard colorimetric card to determine the concentration of ammonia nitrogen in the test solution; if ammonia nitrogen is present in the water, a blue-green color is displayed, and the depth of the color is related to the concentration of ammonia nitrogen in the water.

In the case where the system includes a test paper and a standard colorimetric card, the application method of the system includes: dropping a drop of the solution to be tested on the test paper, leaving it to stand for 3 to 5 minutes, and comparing the color displayed on the test paper with the standard colorimetric card to determine the concentration of ammonia nitrogen in the solution to be tested. The color displayed on the test paper deepens as the concentration of ammonia nitrogen increases, and the color changes from light blue to dark blue, which can determine the concentration of ammonia nitrogen in the solution to be tested.

The present invention is described in detail below in conjunction with specific implementation modes.

Embodiment 1:

The preparation of the ammonia nitrogen detection kit is as follows:

1. Prepare the raw materials in the following proportions: 250g sodium phosphate, 100g potassium sodium tartrate, 50g stabilizer, 150g sodium salicylate, 10g sodium dichloroisocyanurate, 3g potassium nitrosoferrocyanide. Add the above materials into a glass mortar and grind them thoroughly until all the raw materials can pass through an 80-mesh standard sieve. Then, package them into 0.1g tubes and seal them with vacuum or inert gas.

2. When using, add 2mL of water sample into a tube of reagent. If ammonia nitrogen exists in the water, it will show blue-green color. The depth of color is related to the concentration of ammonia nitrogen in the water.

3. Preparation of standard colorimetric card: accurately weigh ammonium sulfate (dried at 110℃ for 2h) and dissolve it in ammonia-free water to prepare ammonia nitrogen standard solutions with concentrations of 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.75mg/L, 1.0mg/L, 1.5mg/L, 2.0mg/L, 4.0mg/L, 6.0mg/L, 8.0mg/L, 10mg/L, 12mg/L, 14mg/L, 16mg/L, 18mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L. Take 2mL of various ammonia nitrogen standard solutions, add 0.1g of solid reagent respectively, seal the packages, shake well, and the color will be basically stable after 5 minutes of color development to obtain the standard colorimetric card.

The preparation of ammonia nitrogen test paper is as follows:

1. Preparation of reagent solution: 250g sodium phosphate, 100g potassium sodium tartrate, 50g stabilizer, 150g sodium salicylate, 10g sodium dichloroisocyanurate, 3g potassium nitrosoferrocyanide. Add the above materials into a glass mortar and grind them thoroughly until all raw materials can pass through an 80-mesh standard sieve. Then divide them into 0.1g per tube and dissolve them in 5mL of ammonia-free water.

2. Immersion: Immerse all the quantitative medium-speed filter paper in the above solution and oscillate on a constant temperature oscillator for 10 to 120 minutes;

3. Molding: Take out the filter paper, place it parallel to each other at room temperature, hang it in the air to dry, cut it and seal it in plastic for later use.

4. Usage: drop a drop of the solution to be tested on the test paper, let it stand for 3 to 5 minutes, compare the color displayed on the test paper with the standard colorimetric card. The color displayed on the test paper deepens with the increase of ammonia nitrogen concentration, and the color changes from light blue to dark blue, which can determine the concentration of ammonia nitrogen in the sample being tested.

Preparation of standard colorimetric card: accurately weigh ammonium sulfate (dried at 110℃ for 2h) and dissolve it in ammonia-free water to prepare ammonia nitrogen standard solutions with concentrations of 0.1mg/L, 0.2mg/L, 0.5mg/L, 0.75mg/L, 1.0mg/L, 1.5mg/L, 2.0mg/L, 4.0mg/L, 6.0mg/L, 8.0mg/L, 10mg/L, 12mg/L, 14mg/L, 16mg/L, 18mg/L, 20mg/L, 50mg/L, 100mg/L, 200mg/L, 500mg/L and 1000mg/L. Take one drop of various ammonia nitrogen standard solutions and add them to ammonia nitrogen test paper respectively. Let them stand for 3 to 5 minutes to prepare standard colorimetric cards.

Those skilled in the art can understand that the above-mentioned embodiments are specific examples for implementing the present application, and in practical applications, various changes can be made to them in form and detail without departing from the spirit and scope of the present application. Any person skilled in the art can make their own changes and modifications without departing from the spirit and scope of the present application, so the scope of protection of the present application shall be based on the scope defined in the claims.

Claims (9)

A kit/test paper for detecting ammonia nitrogen, characterized in that the reagent in the kit/test paper comprises the following component raw materials in parts by mass: 50-500 parts of a buffer, 0-500 parts of a masking agent, 0-500 parts of a stabilizer, 50-500 parts of a developer, 2-100 parts of an oxidant and 1-100 parts of a catalyst; The specification of the reagent is 0.1g per tube; Wherein, the buffer is to maintain the pH of the reagent greater than 12 after being dissolved in 2 mL to 10 mL of water; The masking agent is at least one of sodium citrate, sodium potassium tartrate, sodium hexametaphosphate, DCTA, sodium EDTA salt, EGTA, sodium tartrate or sodium phosphate; The stabilizer is at least one of sodium chloride, sodium sulfate, sodium phosphate, potassium chloride, potassium sulfate, potassium phosphate, and sodium citrate; The developer is salicylic acid or sodium salicylate; The oxidant is at least one of sodium hypochlorite, bleaching powder, sodium dichloroisocyanurate or chlorine dioxide; The catalyst is at least one of cyanocyanate, sodium nitrosoferrocyanide, potassium nitrosoferrocyanide or magnesium nitrosoferrocyanide. The kit/test paper for detecting ammonia nitrogen according to claim 1, characterized in that the buffer is at least one of disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium hydroxide, potassium hydroxide, borax, sodium carbonate, sodium bicarbonate, boric acid, potassium chloride, borax, sodium phosphate, potassium phosphate, glycine, sodium dimethylaminoacetate or sodium diethylbarbiturate. The kit/test paper for detecting ammonia nitrogen according to claim 1 is characterized in that the storage conditions of the kit/test paper are a cool and dry place, and the storage temperature is lower than 26°C. The method for preparing a kit/test paper for detecting ammonia nitrogen according to any one of claims 1 to 3, characterized in that the kit/test paper comprises a kit and a test paper; The preparation method of the kit comprises the following steps: Take the raw materials of each component according to the ratio, grind them sufficiently until they can pass through an 80-mesh standard sieve and mix them evenly, and then pack them into 0.1 g per tube to obtain a reagent; The reagent is evacuated or sealed with an inert gas to obtain the kit; The preparation method of the test paper comprises the following steps: Take the raw materials of each component according to the ratio, grind them sufficiently until they can pass through an 80-mesh standard sieve and mix them evenly, and then pack them into 0.1 g per tube to obtain a reagent; Dissolve the reagent in 1 mL to 5 mL of ammonia-free water to obtain a reagent solution; Immerse all the quantitative medium-speed filter paper in the reagent solution and oscillate on a constant temperature oscillator for 10 minutes to 120 minutes; The filter paper is taken out, placed in parallel at room temperature, hung in the air to dry, cut and sealed in plastic for later use, thereby obtaining the test paper. An ammonia nitrogen detection system, characterized in that the system comprises the kit/test paper for detecting ammonia nitrogen as described in claim 1 or 2, and a standard colorimetric card. The method for preparing an ammonia nitrogen detection system according to claim 5, characterized in that the method for preparing the system comprises: Prepare the kit or test paper, and prepare the standard colorimetric card to obtain the system; Wherein, in the preparation of the standard colorimetric card, the method specifically comprises: drying ammonium sulfate at a temperature of 110° C. for 2 hours, then dissolving it in ammonia-free water, and preparing ammonia nitrogen standard solutions with concentrations of 0.1 mg/L, 0.2 mg/L, 0.5 mg/L, 0.75 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L, 4.0 mg/L, 6.0 mg/L, 8.0 mg/L, 10 mg/L, 12 mg/L, 14 mg/L, 16 mg/L, 18 mg/L, 20 mg/L, 50 mg/L, 100 mg/L, 200 mg/L, 500 mg/L, and 1000 mg/L, taking 1 mL to 5 mL of various ammonia nitrogen standard solutions, respectively adding 0.1 g of solid reagent, sealing the packages with covers, shaking well, and the color is basically stable after 5 minutes of color development to obtain a standard colorimetric card. The application method of the ammonia nitrogen detection system according to claim 5 is characterized in that, when the system includes a kit and a standard colorimetric card, the application method of the system includes: Add 1 mL to 5 mL of the solution to be tested into a tube of the kit, and compare the depth of the blue-green color of the kit with the standard colorimetric card to determine the concentration of ammonia nitrogen in the solution to be tested; In the case where the system includes test strips and a standard colorimetric card, the method of using the system includes: Drop a drop of the solution to be tested on the test paper, let it stand for 3 to 5 minutes, and compare the color displayed on the test paper with the standard colorimetric card to determine the concentration of ammonia nitrogen in the solution to be tested. Use of the kit/test paper for detecting ammonia nitrogen as described in any one of claims 1 to 3 in the field of water quality testing. Application of the ammonia nitrogen detection system as described in claim 5 in the field of water quality detection.