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WO2023018360A3 - Method and apparatus for obtaining autologous cardiomyoblasts - Google Patents

Method and apparatus for obtaining autologous cardiomyoblasts Download PDF

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Publication number
WO2023018360A3
WO2023018360A3 PCT/RU2022/050321 RU2022050321W WO2023018360A3 WO 2023018360 A3 WO2023018360 A3 WO 2023018360A3 RU 2022050321 W RU2022050321 W RU 2022050321W WO 2023018360 A3 WO2023018360 A3 WO 2023018360A3
Authority
WO
WIPO (PCT)
Prior art keywords
patient
cardiomyoblasts
autologous
inductors
terminals
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/RU2022/050321
Other languages
French (fr)
Russian (ru)
Other versions
WO2023018360A2 (en
Inventor
Александр Григорьевич ВИЛЛЕР
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from RU2021123802A external-priority patent/RU2785449C1/en
Application filed by Individual filed Critical Individual
Priority to DE212022000265.2U priority Critical patent/DE212022000265U1/en
Priority to CA3231461A priority patent/CA3231461A1/en
Publication of WO2023018360A2 publication Critical patent/WO2023018360A2/en
Publication of WO2023018360A3 publication Critical patent/WO2023018360A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Physics & Mathematics (AREA)
  • Cardiology (AREA)
  • Rheumatology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Magnetic Treatment Devices (AREA)

Abstract

The invention relates to a method of differentiating a patient's mesenchymal stem cells into cardiomyoblasts, which includes cultivating said stem cells within an artificially generated electromagnetic field autologous to the patient, and further relates to a device comprising: a stand-alone working chamber for accommodating a vessel containing cell cultures; an analog signal generator; and a software processing module. Arranged on opposite walls of the working chamber are a pair of inductors, and metal plates with terminals. The signal generator is designed to generate unmodulated electrical current and voltage signals which simulate the temporal variation of a patient's electrocardiogram or magnetocardiogram signal and which are fed to the inductors and to the terminals of the metal plates according to a predetermined program in order to generate an electromagnetic field that is autologous to the patient. The obtained cardiomyoblasts can be used for delivery to the patient's cardiovascular system to treat heart diseases related to heart tissue deficiency, damage and/or loss.
PCT/RU2022/050321 2021-08-10 2022-10-09 Method and apparatus for obtaining autologous cardiomyoblasts Ceased WO2023018360A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE212022000265.2U DE212022000265U1 (en) 2021-08-10 2022-10-09 Device for obtaining autologous cardiomyoblasts
CA3231461A CA3231461A1 (en) 2021-08-10 2022-10-09 Method and apparatus for obtaining autologous cardiomyoblasts

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2021123802A RU2785449C1 (en) 2021-08-10 Method and apparatus for producing autologous cardiomyoblasts
RU2021123802 2021-08-10

Publications (2)

Publication Number Publication Date
WO2023018360A2 WO2023018360A2 (en) 2023-02-16
WO2023018360A3 true WO2023018360A3 (en) 2023-04-13

Family

ID=85200936

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/RU2022/050321 Ceased WO2023018360A2 (en) 2021-08-10 2022-10-09 Method and apparatus for obtaining autologous cardiomyoblasts

Country Status (3)

Country Link
CA (1) CA3231461A1 (en)
DE (1) DE212022000265U1 (en)
WO (1) WO2023018360A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060057693A1 (en) * 2003-08-20 2006-03-16 Ebi, L.P. Methods of treatment using electromagnetic field stimulated stem cells
US20190126036A1 (en) * 2015-05-05 2019-05-02 National University Of Singapore System and method for applying pulsed electromagnetic fields

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060057693A1 (en) * 2003-08-20 2006-03-16 Ebi, L.P. Methods of treatment using electromagnetic field stimulated stem cells
US20190126036A1 (en) * 2015-05-05 2019-05-02 National University Of Singapore System and method for applying pulsed electromagnetic fields

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FATHI EZZATOLLAH, FARAHZADI RAHELEH: "Enhancement of osteogenic differentiation of rat adipose tissue-derived mesenchymal stem cells by zinc sulphate under electromagnetic field via the PKA, ERK1/2 and Wnt/p-catenin signaling pathways", PLOS ONE, vol. 12, no. 3, 24 March 2017 (2017-03-24), pages e0173877, XP093060988 *
KASPARICK M. ET AL.: "Mechanism for safe remote activation of networked surgical and PoC devices using dynamic assignable controls", ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY. IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, August 2016 (2016-08-01), pages 2390 - 2394, XP032979657, DOI: 10.1109/EMBC.2016.7591211 *
KOLESNIKOV V. V., SALNIKOVA E. N.: "Konstruktsiia fotometricheskogo pulsoksimetra dlia mochki ukha s besprovodnoi sviaziu [Design of a photometric earlobe pulse oximeter with wireless communication]", MOLODOI UCHENY, vol. 116, no. 12 (116), 30 November 2015 (2015-11-30), pages 298 - 300, XP009546007, ISSN: 2072-0297 *

Also Published As

Publication number Publication date
DE212022000265U1 (en) 2024-11-28
CA3231461A1 (en) 2023-02-16
WO2023018360A2 (en) 2023-02-16

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