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WO2021112298A1 - Procédé de production d'un produit de fermentation complexe de minéraux dérivés de lichens et de galactomyces - Google Patents

Procédé de production d'un produit de fermentation complexe de minéraux dérivés de lichens et de galactomyces Download PDF

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Publication number
WO2021112298A1
WO2021112298A1 PCT/KR2019/017140 KR2019017140W WO2021112298A1 WO 2021112298 A1 WO2021112298 A1 WO 2021112298A1 KR 2019017140 W KR2019017140 W KR 2019017140W WO 2021112298 A1 WO2021112298 A1 WO 2021112298A1
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Prior art keywords
galactomyces
complex
lichen
minerals
derived
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English (en)
Korean (ko)
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전희형
문제헌
심성보
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Nature In Lab Inc
Urg Inc
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Nature In Lab Inc
Urg Inc
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9728—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9733—Lichens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Definitions

  • the present invention relates to fermentation by adding a complex containing at least one of minerals (Cu, Zn, Mg, Ca, Se, etc.) derived from lichens during Galactomyces fermentation.
  • Lichen-derived mineral extraction method and Galactomyces complex comprising the same, characterized in that it controls the osmotic balance of skin cells, activates keratin metabolism, relieves inflammation, and provides moisturizing effects using a Galactomyces complex fermented product It relates to the fermentation method.
  • Minerals also called minerals, are elements that make up the human body and are not decomposed unlike organic substances that are decomposed when ingested.
  • the minerals that make up the human body are potassium (K), calcium (Ca), selenium (Se), sodium (Na), iodine (I), zinc (Zn), magnesium (Mg), phosphorus (P), sulfur (S), There are chlorine (Cl), copper (Cu), manganese (Mn), iron (Fe), cobalt (Co), etc., which cannot be synthesized in the body and must be consumed through food. It accounts for about 4% of the body and is involved in various functions such as bone and tooth composition, blood oxygen transport, digestion and osmotic pressure regulation.
  • the minerals are involved in various metabolic processes of the body to maintain a healthy body, but modern people often lack or excessive certain minerals and thus are unbalanced.
  • they suffer from itching of unknown causes, including atopic dermatitis, dry skin, and the like (pruritus, pruritus).
  • the atopic itch is known to be due to histamine being unnecessarily discharged from the skin due to an abnormality in the immune system, but in most cases, it is difficult to clearly identify the cause.
  • skin-related diseases occur because the skin barrier is damaged and the skin is not moisturized in time and is easily exposed to the attack of external bacteria or viruses.
  • natural minerals play a role in resolving skin troubles, and do not directly kill viruses or bacteria, but reproduce. It has a function to prevent it from doing so after a certain period of time, and it has been found to have no side effects because it is a natural method.
  • natural minerals are known to increase skin moisture by activating natural moisturizing factor (NMF) and to prevent skin irritation by activating enzymes that remove active oxygen (SOD).
  • lichens are symbiotic complexes of fungi and algae or/and cyanobacteria, and are classified into about 14,000 species worldwide. It was once proposed to use secondary metabolites of lichens as antimicrobial agents or antiherbivore agents. In addition, some lichen extracts have been used as various therapeutic agents in the folklore, and lichen metabolites have antibiotic agent, antimycobacterial, antiviral, analgesic, antipyretic properties, etc. has been shown to have a variety of biological activities. At the same time, research results show that minerals extracted from lichens have an excellent effect in preventing skin aging due to cellular ion imbalance.
  • the fermented product of the fermented strain was used to improve skin moisturizing.
  • Fermented products are known to have a function of activating natural moisturizing factor (NMF) to supply moisture to skin cells and help form a natural moisturizing barrier, so it is used as a cosmetic ingredient for moisture supply.
  • NMF natural moisturizing factor
  • Galactomyces is one of the natural yeasts fermented with yeast.
  • Interest in the effectiveness and various active ingredients contained therein has increased. It started to attract attention as it was contained in the cosmetic brand SK-II products, and various vitamins such as vitamins B1, B2, pantothenic acid, B6, folic acid, carbohydrates, and various amino acids that are natural moisturizing factors that provide skin moisturizing, whitening, pores and soothing effects and rich in various minerals.
  • the anti-aging effect of nucleic acid which contains more than 7 times that of sardines, is known, and kojic acid, which is known as a representative whitening ingredient, was first known in the early 1900's in the process of fermenting steamed rice (koji) with yeast.
  • various natural enzymes such as saccharification enzymes and proteases produced during the fermentation process are known to have antioxidant, anti-aging, whitening, and skin moisturizing effects by increasing the production and absorption of active substances.
  • the present inventors have made continuous efforts to develop a cosmetic composition that improves the skin barrier and alleviates mineral imbalance from lichen extracts, and as a result, it has been found that natural minerals derived from lichens are composed of various components, and fermented strains Found that galactomyces fermented product contains a large amount of organic matter including natural moisturizing factors, the fermented galactomyces fermented material added with the above lichen-derived minerals has the effect of improving the skin barrier and resolving cellular ion imbalance was found to complete the present invention.
  • the present invention provides a method for producing a galactomyces complex fermented product by adding lichen-derived minerals.
  • the step of culturing the inoculum in the medium (S21); inoculating and culturing galactomuces in the culture medium of the inoculum (S22); adding lichen-derived minerals (S23); After adding the lichen-derived minerals, further culturing (S24); disrupting the cells obtained by the additional culture (S25); and centrifuging and filtering the crushed cells (25); provides a method for producing a galactomyces complex fermented product comprising a.
  • the inoculum is yeast bacteria (Saccharomyces), lactic acid bacteria (Lactobacillus), Bacillus subtilis (Bacillus), yeast (Aspergillus) or photosynthetic bacteria (Rhodobacter) galactomyces (galactomyces) containing any one or more selected from the group comprising ) provides a method for producing a complex fermented product.
  • the step of inoculating and culturing galactomyces (galactomuces) in the culture medium in which the inoculum is inoculated with the galactomyces (galactomuces) at a specific gravity of 5% to 10% relative to the total proportion occupied by the inoculum of the medium Provided is a method for preparing a galactomyces complex ferment.
  • the lichen-derived mineral is selected from the lichen (S11); washing and drying the selected lichen (S12); grinding the washed and dried lichens (S13); extracting a mineral complex from the pulverized product (S14); Desalting and filtering the mineral complex (S15); and lyophilizing after desalting and filtration (S16); provides a method for producing a fermented product comprising a galactomyces complex.
  • the lichen-derived minerals are Umbilicaria antarctica, Peltigera canina (Peltigera canina), Peltigera praetextata (Peltigera praetextata), Lamarina conduprikans (Ramalina, conduplicans), Ramalina intermedia, Xanthoparmelia farinose, Diplocia canescens, Cetraria aculeate, Cladonia furcata, sudepe Pseudephebe pubescens, Sphaerophorus globosus, Stereocaulon alpinum, Umbilicaria antarctica, Usnea antarctica Any one or more selected from the group comprising , Usnea longissima, Usnea barbata and Usnea aurantiacoatra or Stictanylanderiana Provided is a method for producing a galactomyces complex fermented product comprising minerals derived from lichens.
  • the lichen-derived minerals are from the group comprising Na, Fe, K, Mg, Al, Mn, Si, Ca, Zn, I, S, P, Se, Mg or pharmaceutically or cosmetically acceptable minerals thereof.
  • a method for producing a galactomyces complex fermented product comprising one or more selected minerals.
  • 0.01 wt% to 0.5 wt% of the lichen-derived mineral is added in an amount of 0.01 wt% to 0.5 wt% compared to the medium in which the inoculum and galactomuces are mixed and cultured galactomyces (galactomyces) ) provides a method for producing a complex fermented product.
  • the additional culturing step provides a method for producing a galactomyces (galactomyces) complex fermentation, characterized in that the additional culture at 34 °C to 36 °C 12 hours to 48 hours.
  • the washing and drying step provides a method for producing a galactomyces complex fermented product, characterized in that the lichen is washed with purified water and then dried to a moisture content of 10% or less.
  • the pulverizing step provides a method for producing a galactomyces (galactomyces) complex fermented product, characterized in that pulverized to a size of 350um to 2,000um.
  • the step of extracting the mineral complex from the pulverized product is a step of dispersing the pulverized product at 0.05 wt% to 0.5 wt% relative to purified water, and stirring at 60 ° C to 120 ° C for 1 h to 6 h at 5 rpm to 30 rpm.
  • a method for a galactomyces complex ferment comprising
  • the step of extracting with stirring includes adding any one or more organic acids selected from the group including malic acid (malic acid), citric acid (citric acid), fumaric acid, tartaric acid, oxalic acid, propionic acid or lactic acid.
  • malic acid malic acid
  • citric acid citric acid
  • fumaric acid fumaric acid
  • tartaric acid tartaric acid
  • oxalic acid propionic acid or lactic acid.
  • galactomyces provides a method for preparing a complex fermented product.
  • the organic acid provides a method for producing a galactomyces (galactomyces) complex fermented product, characterized in that 0.01% to 2.0% by weight compared to the mixture consisting of the purified water and the pulverized product.
  • the fermented Galactomyces complex cultured by adding the mineral complex derived from lichens of the present invention has effects of regulating the osmotic balance of skin cells, activating brown skin metabolism, relieving inflammation and moisturizing.
  • FIG. 1 is a flowchart illustrating a method for manufacturing a complex fermented product of lichen-derived minerals and galactomyces according to an embodiment of the present invention.
  • FIG. 2 shows Il-6 values generated during hypertonic solution stress to prove the effect of regulating osmotic pressure balance in hypertonic solution according to an embodiment of the present invention.
  • Figure 3 shows Il-33 values generated during storage solution stress to prove the effect of regulating the osmotic pressure balance in the stock solution according to an embodiment of the present invention.
  • TEWL transdermal water loss
  • the method for producing a fermented Galactomyces complex containing lichen-derived minerals includes a method of extracting minerals from lichens and culturing galactomyces to which lichen-derived minerals are added to prepare a complex fermented material. It is composed of a method for obtaining, and the complex fermented material provides an effect of improving skin health by controlling osmotic balance, activating keratin metabolism, alleviating inflammation, and moisturizing.
  • the method of extracting minerals from lichens may be preceded, and the method for extracting minerals from lichens The method may start from the step S11 of selecting a lichen.
  • the minerals added to the galactomyces complex fermented product manufacturing method are derived from lichens, and the lichens are defined as a symbiotic complex of fungi and algae or / and cyanobacteria. .
  • Lichens of the present invention are lichens that are native to the polar or alpine regions, and are Umbilicaria antarctica, Peltigera canina, Peltigera praetextata, Lamarina condu. Freecans (Ramalina, conduplicans), Ramalina intermedia, Xanthoparmelia farinose, Diplocia canescens, Cetraria aculeate, Cladonia pur Cata (Cladonia furcata), Pseudephebe pubescens, Sphaerophorus globosus, Stereocaulon alpinum, Umbilicaria antarctica, Us Includes Usnea antarctica, Usnea longissima, Usnea barbata and Usnea aurantiacotra or Stictanylanderiana, etc. Any one or more lichens selected from the group
  • the Umbilicaria antarctica is a lichen collected from King George Island in Antarctica by researchers, Usnea longissima is a lichen native to Mt. It is a lichen collected from the alpine region of Yunnan, China. These lichens are a kind of lichen that live in an environment unsuitable for normal living things, such as strong and dry winds, irregular fluctuations in moisture and nutrients, and strong ultraviolet rays throughout the year.
  • the lichen-derived minerals added to the Galactomyces culture of the present invention refer to minerals, and calcium (Ca), phosphorus (P), potassium (K), iron (Fe), zinc (Zn), copper ( Cu), manganese (Mn), selenium (Se), etc. are contained. Minerals are essential for maintaining healthy cells and the human body, such as improving the skin health of modern people, especially atopic symptoms, and delaying skin aging.
  • zinc plays a very important function in maintaining and regenerating the skin
  • selenium acts as an antioxidant and an essential component of glutathione peroxidase, a type of antioxidant enzyme. It has been reported that it inhibits the production of caine and causes anti-inflammatory action.
  • phosphorus (P) is involved in acid-alkali balance within the skin cells and buffers the skin cell fluid. It contributes to the equilibrium, maintains the osmotic pressure inside and outside the skin cells, and resolves the ion imbalance.
  • Another mineral, iron (Fe) binds to oxygen and maintains the metabolism of all tissue cells, and when it is deficient, it causes glossitis, cheilitis, spoon-type nail of the nail and hair loss, and copper (Cu) It activates the melanin-forming enzyme, and when it is deficient, it causes torsion hair, thermophilia nodosa, and bead hair.
  • mineral refers to a mineral extracted from or separated from lichens by a conventional method.
  • mineral refers to a material produced through the extraction process, desalination and filtration process or drying process, and the calcium (Ca), phosphorus (P), potassium (K), iron (Fe),
  • general minerals such as zinc (Zn), copper (Cu), manganese (Mn), and selenium (Se)
  • vitamins, organic substances, etc. may be included in the extraction process.
  • Mineral may be used interchangeably with “mineral complex”.
  • a step (S12) of washing and drying the selected lichen may be included, and the washing and drying step (S12) of the selected lichen is washed with purified water and then the moisture content is about 10 % or less may be dried. If the moisture content is less than 10%, it is easy to pulverize the lichen in the subsequent grinding step (S13). However, if the moisture content is more than 10%, aggregation occurs between the pulverized materials of the lichen and requires a lot of power to grind, so it is inefficient in terms of productivity. to be.
  • the pulverizing step (S13) is a step of pulverizing the lichen pulverized material to a predetermined size, and may be pulverized to a size of 350 ⁇ m to 2,000 ⁇ m with a grinder such as a stainless mixer.
  • the pulverization step (S13) may include a step (S14) of extracting the mineral complex from the pulverized product.
  • the pulverized material pulverized to a size of 350um to 2,000um is dispersed in purified water to be 0.05% to 0.5% by weight compared to purified water, and 5rpm to 30rpm at 60°C to 120°C for 1h to 6h. It may consist of a step of extracting by stirring.
  • the reason for dispersing the pulverized product in purified water so as to be 0.05 wt% to 0.5 wt% compared to purified water is to optimize the extraction of minerals contained in the pulverized product, and when it is 0.05 wt% or less, the content of extracted minerals is too low There is a problem in that the efficiency is not good because there is a large amount lost in the post-treatment process, and when it is 0.5 wt% or more, a significant portion of the minerals contained in the pulverized product cannot be extracted.
  • it may consist of a step of extracting by stirring at 5 rpm to 30 rpm at 60° C. to 120° C. for 1 h to 6 h.
  • the temperature is 60° C. or less, the efficiency of the extracted minerals decreases, and when it is 120° C. or more, pulverization Water is denatured due to high temperature, and unnecessary components other than the expected components are extracted, complicating the post-treatment process and increasing the cost.
  • the extraction step (S14) may be composed of a step of extracting the mineral complex by further adding one or more organic acids.
  • an inorganic acid such as hydrochloric acid
  • the extraction efficiency of the mineral complex can be increased, but since there is a problem that the inorganic acid remains in the extract after mineral extraction, an organic acid can be used instead of the inorganic acid to solve the residual accumulation problem and ensure stability. have.
  • the organic acid may include any one or more organic acids selected from the group consisting of malic acid (malic acid), citric acid (citric acid), fumaric acid, tartaric acid, oxalic acid, propionic acid or lactic acid.
  • the organic acid Since the organic acid has a lower mineral extraction efficiency than that of a strong acid, it is important to find an appropriate extraction condition.
  • the organic acid is preferably added in an amount of 0.01 wt% to 2 wt% based on the total of the mixture of the pulverized product and purified water.
  • the organic acid concentration is 0.01% to 2% by weight, the yield reaches the highest value, but when it is 2% by weight or more, it is not economical because a constant yield is shown without increasing the yield, and when it is 0.01% by weight or less, the extraction time is long. have. Therefore, it is preferable to appropriately adjust the organic acid concentration within the range of 0.01 wt% to 2 wt% in consideration of the pH conditions according to the use.
  • the extraction step (S14) may include a step (S15) of desalting and filtering the extracted mineral complex.
  • the desalting step is a step of removing salts from the mineral complex that has undergone the extraction step (S14) to produce mineral water, which is to remove salts corresponding to impurities.
  • the desalting step may be performed by methods such as a desalting treatment method by an electric extraction method and a desalting treatment method by an electrodialysis method, and is not limited to a specific method.
  • the filtration step is a process of removing suspended solid particles from the extract, and can be separated using the size of the particles passing through the membrane.
  • nanofiltration and microfiltration are used.
  • ultrafiltration and reverse osmosis.
  • an ultrafiltration device that usually has a separation target in the range of 1 nm to 0.1 ⁇ m.
  • a freeze-drying step (S16) may be included.
  • the drying step there are drying methods such as hot air drying or freeze drying, spray drying, and reduced pressure drying, but considering the efficacy of the dried mineral complex, the freeze-dried product is advantageous in terms of efficacy compared to other drying methods, so the drying method in the present invention is A lichen-derived mineral complex can be obtained by using a freeze-drying method.
  • the method for producing a complex fermented material by culturing galactomyces to which lichen-derived minerals are added may be constituted from the step of culturing the inoculum (S21).
  • the inoculum is any one or more selected from the group comprising yeast (Saccharomyces), lactic acid bacteria (Lactobacillus), Bacillus subtilis (Bacillus), yeast (Aspergillus) or photosynthetic bacteria (Rhodobacter) can be composed of
  • yeast Sacharomyces
  • lactobacillus promotes the production of 'loricrin' protein, which occupies more than 70% of the stratum corneum of the skin, and has a function to strengthen the skin barrier by increasing healthy keratinocytes. It protects the skin from harmful bacteria, inhibits the growth of harmful bacteria, and has excellent anti-inflammatory effects.
  • Bacillus Bacillus produces a large amount of butanediol and has the ability to hold moisture, thereby improving skin moisture.
  • the kojic acid of Aspergillus is used as a functional cosmetic composition because it has a blemish-repellent effect and contains a large amount of various vitamins, amino acids, minerals, organic acids, and the like.
  • Photosynthetic bacteria (Rhodobacter) is used as a cosmetic composition for the treatment of skin diseases by producing an anti-inflammatory substance.
  • Galctomyces may be inoculated with a specific gravity of 5% to 10% of the total weight of the culture medium in this fermenter. If Galctomyces is inoculated at less than 5%, there is a possibility that the fermentation may not proceed sufficiently due to insufficient amount of inoculation bacteria. If it is added in excess of 10%, the amount of inoculation bacteria is too large and oxygen is lost. Insufficient fermentation does not occur, and there is a problem that the culture medium itself is corrupted or overfermented, so it is preferable to inoculate it with a specific gravity of 5% to 10%.
  • the fermented Galactomyces complex is a natural material containing a large amount of organic substances and can be obtained by fermenting lichen-derived minerals with a Galactomyces strain.
  • the fermented product of Galactomyces can improve skin moisture by promoting the conversion of profilagrin, a precursor of natural moisturizing factor (NMF), to natural moisturizing factor in water.
  • profilagrin a precursor of natural moisturizing factor (NMF)
  • NMF natural moisturizing factor
  • the abundant polysaccharide component contained in the fermented product of Galactomyces prevents moisture evaporation within the skin surface, and is rich in various vitamins such as vitamins B1, B2, pantothenic acid, B6, and folic acid.
  • Various natural enzymes such as protease, a glycosylation enzyme, increase the production and absorption of active substances, and thus have antioxidant, anti-aging, whitening, and skin moisturizing effects.
  • the step of inoculating and culturing Galactomyces is preferably incubated at 34 ° C. to 36 ° C. for 24 hours to 36 hours. If the incubation time is less than 24 hours, Galctomyces cannot be sufficiently cultured, and if it exceeds 36 hours, Galctomyces is overcultured. There is a problem in that fermentation efficiency is lowered due to an increase in the ratio of Galactomyces having low activity.
  • the lichen-derived mineral complex obtained through the freeze-drying step (S16) after inoculating and culturing the Galactomyces (S22).
  • the lichen-derived mineral complex is preferably added in an amount of 0.01 wt% to 0.5 wt% compared to the mixed culture medium of the inoculum and galactomyces. If it is less than 0.01% by weight, the effect of the active ingredient of the lichen mineral complex is insignificant, and if it exceeds 0.5% by weight, the excess mineral affects the physiological activity of Galactomyces, and the fermentation efficiency is lowered. to be.
  • the additional culturing step (S24) may include a step (S24) of additional culturing after the step (S23) of adding the lichen-derived mineral complex.
  • the additional culturing step (S24) is preferably incubated at 34°C to 36°C for 12 hours to 48 hours. When the culture time is less than 12 hours, sufficient fermentation time is not provided, and when it exceeds 48 hours, the change in the components of the fermented product obtained relative to the fermentation time is insignificant.
  • it may include a step (S25) of crushing the cells obtained through the additional culturing step (S24).
  • it may include a step (S26) of centrifugation and filtration after the step of crushing the cells (S25).
  • a step (S26) of centrifugation and filtration after the step of crushing the cells (S25).
  • a cosmetic composition containing a complex fermented product obtained by the manufacturing method of the present invention wherein the cosmetic composition has an osmotic balance control, keratin metabolism activation, inflammation relief or moisturizing effect. It can be usefully used as a functional cosmetic.
  • a species of Usnea barbata native to Yunnan, China was selected (S11). Thereafter, the selected lichens were washed with purified water and dried to a moisture content of about 10% or less (S12). Thereafter, lichens were pulverized to a size of 350 ⁇ m to 2,000 ⁇ m with a stainless mixer (S13). Thereafter, purified water is dispersed so that the pulverized product pulverized with a mixer becomes 0.3% by weight relative to purified water, malic acid, one of the organic acids, is added to 1% by weight compared to the pulverized product and purified water mixture, and 3 hours at 100° C. The mineral complex was extracted by stirring at 15 rpm during (S14). Thereafter, after desalting by an electric extraction method, it was filtered with an ultrafiltration device (Pall, SLP-3053) (S15). Then, lyophilized to obtain a mineral complex derived from lichens (S16)
  • Example 1 In the inoculum culture step (S21) of Example 1, it was prepared in the same manner as in Example 1, except that it was replaced with Lactobacillus.
  • Example 1 In the inoculum culture step (S21) of Example 1, it was prepared in the same manner as in Example 1, except that it was replaced with Bacillus.
  • Example 1 In the inoculum culture step (S21) of Example 1, it was prepared in the same manner as in Example 1, except that it was replaced with Aspergillus.
  • Example 1 In the inoculum culture step (S21) of Example 1, it was prepared in the same manner as in Example 1, except that the photosynthetic bacteria (Rhodobacter) was replaced.
  • the photosynthetic bacteria Rhodobacter
  • Example 1 In the lichen selection step (S11) of Example 1, it was prepared in the same manner as in Example 1, except that it was replaced with Pseudephebe pubescens, which is a lichen collected from an alpine region of Yunnan, China.
  • Example 2 It was prepared in the same manner as in Example 1, except that in Example 1, the extraction of lichen-derived minerals (S11 to S16) and the step (S23) of adding the lichen-derived mineral complex to the fermentation medium were omitted.
  • the mineral analysis of the Galactomyces complex fermented product cultured with the addition of the lichen-derived minerals of Examples 1 to 6 and the Galactomyces complex fermented product cultured without the addition of the lichen-derived mineral prepared in Comparative Example is inductively coupled plasma mass spectrometry mass Mineral components and content were analyzed using an analyzer (ICP-MS, X-series (X5,X7), Thermo Elemental, UK), and the analysis results are shown in Table 1 below.
  • Formulation Examples 1 to 6 are the galactomyces complex fermentation products containing lichen-derived minerals prepared according to the above Examples, and Formulation Examples 1 to 6 are Preparation Examples 1 to 5 Minerals derived from lichens prepared in .
  • a cosmetic standard sample was prepared using the Galactomyces complex fermented product not containing the lichen-derived mineral prepared in Comparative Example.
  • HDFa Human Dermal fibroblasts
  • Medium 106 and Low Serum Growth Supplement in a medium containing penicillin (100IU/ml) and streptomycin (100 ⁇ g/ml) at 37°C in an incubator containing 5% CO2 cultured within.
  • penicillin 100IU/ml
  • streptomycin 100 ⁇ g/ml
  • cells were aliquoted in a 96 well plate and then cultured for 18 hours in cell culture conditions. After removing the medium and washing with PBS, a new medium and a cosmetic standard sample were added, respectively, and incubated for 18 hours. After 18 hours, in order to measure the viability of the cells, the mitochondria activity of the living cells was measured to confirm the cell viability by performing the MTT ASSAY to check the viability of the cells, and the results are shown in Table 3 below.
  • the cosmetic standard samples of Formulation Examples 1 to 6 of the present invention showed a high cell viability of 100% or more.
  • Formulation Example 1 shows the best cell viability.
  • the skin moisture content was measured, and after use, the skin moisture content over time was re-measured.
  • the moisture content increase rate of the skin moisture content and the moisture content before use was measured when 10 minutes elapsed after using the cosmetic standard sample, and the measurement results are shown in Table 4 below.
  • IL-6 and IL-33 expression inhibition experiments were carried out. Specifically, human fibroblasts (Human Dermal fibroblast: HDFa) were inoculated in a Petri dish at a density of 104/cm 2 and cultured for 24 hours, including 200 mmol/L of NaCl for IL-6 expression due to hypertonic stress. was replaced with a hypertonic solution stress medium and cultured for 24 hours.
  • human fibroblasts Human Dermal fibroblast: HDFa
  • the cosmetic standard sample prepared from the Galactomyces complex fermented product with the addition of lichen-derived minerals of the present invention compared to the cosmetic standard sample prepared from the Galactomyces fermented product cultured without the lichen-derived mineral was subjected to hypertonic solution stress ( It can be seen that the reduction range of IL-6 generated during hyper-osmetic stress) is large, confirming that it is advantageous in regulating the balance of hypertonic solution osmotic pressure.
  • sample IL-33 expression level (pg/mL) contrast example Stock Stress(-) / Normal Medium 71 ⁇ 4 comparative example Stock Stress(+) / Normal Medium 148 ⁇ 21
  • TEWameter TM 300 (Courage & Khazaka, Cologne, Germany) was used for transdermal moisture loss. (TEWL, transepidermal water loss) was measured.
  • the cosmetic standard sample prepared from the Galactomyces complex fermented product grown by adding the lichen-derived mineral of the present invention has a lower TEWL level than the cosmetic standard sample prepared from the Galactomyces fermented product cultured without the lichen-derived mineral. It can be seen that the degree of damage to the epidermal permeation barrier due to damage to the stratum corneum is reduced, thereby confirming the effect of improving the skin barrier.
  • the cosmetic standard sample prepared from the Galactomyces complex fermented product grown by adding the lichen-derived mineral of the present invention compared to the cosmetic standard sample prepared from the Galactomyces fermented product cultured without the lichen-derived mineral had an average value before application. , 2 weeks after application and 4 weeks after application, the average value was significantly reduced, so it is judged that the fermented Galactomyces complex cultured with lichen-derived minerals helps to improve keratin.
  • Lipoxygenase activity inhibition experiment was conducted to measure the anti-inflammatory effect of a cosmetic standard sample prepared from a Galactomyces complex fermented product added with the lichen-derived mineral of the present invention. Specifically, 500 ⁇ L of each of the substrate solution (Linoleic acid), the enzyme (5-lipoxygenase) and the fermented Galactomyces were mixed, and 250 ⁇ L of saturated KI solution and 250 ⁇ L of 1% starch reagent were treated to check the peroxide production level. After reacting at 37°C for 30 minutes, absorbance was measured at 540 nm, and the measurement results are shown in Table 9 and FIG. 5 .
  • Example 2 Example 3
  • Example 4 Example 5
  • Example 6 comparative example Inhibition rateof LOX(%) 50.0 23.1 35.4 32.6 37.4 35.2 28.4 43.4 SD 5.0 2.5 3.4 4.5 7.4 5.2 5.4 7.4
  • the Galactomyces complex fermented product cultured with the addition of lichen-derived minerals of the present invention exhibited a higher lipoxygenase activity inhibition rate in the lipoxygenase activity inhibition experiment, which is an anti-inflammatory confirmation experiment, compared to the Galactomyces fermented product cultured without lichen-derived minerals. As shown, it is judged that the fermented Galactomyces complex fermented with the addition of lichen-derived minerals has an anti-inflammatory effect.

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Abstract

La présente invention concerne : un procédé d'extraction de minéraux dérivés de lichens ; et un procédé de production d'un produit de fermentation complexe de galactomyces. Le produit de fermentation complexe de galactomyces est obtenu par ajout d'un complexe minéral, qui comprend au moins un minéral (Cu, Zn, Mg, Ca, et Ge, etc.) dérivé de lichens, dans le procédé de fermentation de galactomyces, et a pour effet de réguler l'équilibre osmotique des cellules de la peau, d'activer le métabolisme de la kératine, de soulager l'inflammation et d'hydrater.
PCT/KR2019/017140 2019-12-05 2019-12-05 Procédé de production d'un produit de fermentation complexe de minéraux dérivés de lichens et de galactomyces Ceased WO2021112298A1 (fr)

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