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WO2019172554A1 - Composition cosmétique de blanchiment de la peau contenant une solution de mélange de cultures de lactobacilles - Google Patents

Composition cosmétique de blanchiment de la peau contenant une solution de mélange de cultures de lactobacilles Download PDF

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Publication number
WO2019172554A1
WO2019172554A1 PCT/KR2019/001951 KR2019001951W WO2019172554A1 WO 2019172554 A1 WO2019172554 A1 WO 2019172554A1 KR 2019001951 W KR2019001951 W KR 2019001951W WO 2019172554 A1 WO2019172554 A1 WO 2019172554A1
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Prior art keywords
culture
lactic acid
acid bacteria
lactobacillus
cosmetic composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2019/001951
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English (en)
Korean (ko)
Inventor
노윤화
이동걸
김미선
김민지
강승현
박명삼
이문영
김효정
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hanmi Pharmaceutical Co Ltd
Cosmax Inc
Hanmi Pharmaceutical Industries Co Ltd
Original Assignee
Hanmi Pharmaceutical Co Ltd
Cosmax Inc
Hanmi Pharmaceutical Industries Co Ltd
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Publication of WO2019172554A1 publication Critical patent/WO2019172554A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

  • the present invention relates to a cosmetic composition containing a culture solution of lactic acid bacteria, a method for preparing the same, a use for the skin whitening thereof, a method for skin whitening using the same, and specifically, Lactobacillus rhamnosus culture, Lactobacillus ashdophyllus culture and
  • the present invention relates to a cosmetic composition for skin whitening containing a lactic acid bacteria culture medium mixed with Bifidobacterium animalis culture.
  • melanin There are many factors that determine human skin color, but the most important factor is melanin.
  • the number of melanocytes that produce melanin pigments is constant regardless of ethnic or skin color, but there are differences in melaninization, size, number and distribution of melanocytes, and the resolution of melanocytes in keratinocytes. The skin color can thus be determined.
  • Melanin is a series of oxidation-polymerizations in which L-tyrosine in melanocytes is converted to L-dopa by a hydrolysis reaction and then to L-dopaquinone by an oxidation reaction. It is synthesized through the reaction, and the melanin produced thus becomes dark in color of the skin. Recently, due to the rapid development of the industry, the air pollution and the ozone layer are severely destroyed, the skin turns black due to melanin pigmentation, and consumers' interest in various functional cosmetics to prevent this is increasing steadily. to be.
  • tyrosinase The most important enzyme in the production of melanin is tyrosinase (tyrosinase), and in general, the expression of tyrosinase increases melanin production.
  • tyrosinase the expression of tyrosinase in cells is regulated by a small microphthalmia-associated transcription factor (MITF), which binds to M-BOX (promoter of tyrosinase) and acts as a transcription factor.
  • MITF transcription factors induce the transcription of not only tyrosinase, but also a series of enzymes involved in melanin biosynthesis, such as RAB27A, which is involved in the transport of melanosomes in melanocytes. Therefore, it is possible to suppress the phenomenon that the skin turns black by melanin pigmentation through the inhibition of the MITF transcription factor.
  • probiotics has an etymological meaning that is opposed to antibiotics (antibiotics), which means antibiotics, and means a microbial agent or microbial component that helps intestinal microbial balance.
  • lactobacillus such as Lactobacillus and Bifidobacteria
  • these probiotics increase the number of useful microorganisms in the genus Lactobacillus and Bifidobacterium, thereby inhibiting the growth of harmful pathogens, and treating antibiotics or changing the external environment. It is known to have an effect of maintaining the homeostasis of the intestinal bacterial flora.
  • the present inventors earnestly tried to develop a composition having a skin lightening effect.
  • lactic acid bacterium mixture mixture of Lactobacillus rhamnosus culture, Lactobacillus ashidophilus culture and Bifidobacterium animalis culture melanin production, migration and secretion inhibitory effect, and synthesis of melanin
  • the present invention has been completed by clarifying that the MITF gene expression reduction effect involved in can be excellent and exhibit a skin whitening effect.
  • Another object of the present invention is to provide a method for preparing a cosmetic composition for skin whitening containing a lactic acid bacteria culture liquid mixture.
  • Another object of the present invention is to provide a skin whitening use of the lactic acid bacteria culture liquid mixture.
  • Still another object of the present invention is to provide a skin whitening method using a lactic acid bacteria culture solution.
  • the present inventors earnestly tried to develop a composition having a skin lightening effect.
  • lactic acid bacterium mixture mixture of Lactobacillus rhamnosus culture, Lactobacillus ashidophilus culture and Bifidobacterium animalis culture melanin production, migration and secretion inhibitory effect, and synthesis of melanin It was found that the effect of reducing MITF gene expression, which is involved in, was excellent, and the skin whitening effect could be exhibited.
  • the present invention relates to a cosmetic composition containing a culture solution of lactic acid bacteria, a method for preparing the same, a use for the skin whitening thereof, a method for skin whitening using the same, and specifically, Lactobacillus rhamnosus culture, Lactobacillus ashdophyllus culture and
  • the present invention relates to a cosmetic composition for skin whitening containing a lactic acid bacteria culture medium mixed with Bifidobacterium animalis culture.
  • One aspect of the present invention relates to a cosmetic composition containing a mixed solution of lactic acid bacteria culture.
  • the cosmetic composition is a cosmetically effective amount of the above-described lactic acid bacteria culture mixture of the present invention (cosmetically effective amount); And / or may include a cosmetically acceptable carrier, but is not limited thereto.
  • cosmetic effective amount means an amount sufficient to achieve the skin condition improving efficacy of the composition of the present invention described above.
  • the lactic acid bacteria may be at least two selected from the group consisting of Lactobacillus rhamnosus , Lactobacillus acidophilus and Bifidobacterium animalis , for example, Lactobacillus It may be, but is not limited to, Rhamnosus, Lactobacillus ashidophyllus and Bifidobacterium animalis.
  • the mixed solution of the lactic acid bacteria culture may be a mixture of two or more selected from the group consisting of Lactobacillus rhamnosus culture, Lactobacillus ashidophilus culture and Bifidobacterium animalis culture, for example , Lactobacillus rhamnosus culture, Lactobacillus ashidophyl culture and Bifidobacterium animalis culture may be mixed.
  • the lactic acid bacteria culture solution may be a mixture of Lactobacillus rhamnosus culture, Lactobacillus ashidophyllus culture and Bifidobacterium animalis culture in a volume of 1: 0.2 to 5: 0.2 to 5, for example, 1: 0.2 to 4: 0.2 to 4, 1: 0.2 to 3: 0.2 to 3, 1: 0.2 to 2: 0.2 to 2, 1: 0.2 to 1.8: 0.2 to 1.8, 1: 0.2 to 1.6: 0.2 To 1.6, 1: 0.2 to 1.4: 0.2 to 1.4, 1: 0.2 to 1.2: 0.2 to 1.2, 1: 0.4 to 1.2: 0.4 to 1.2, 1: 0.6 to 1.2: 0.6 to 1.2, 1: 0.8 to 1.2: 0.8 To 1.2 or 1: 1: 1, but may not be limited thereto.
  • the concentration of the lactic acid bacteria culture liquid mixture contained in the cosmetic composition may be 0.01 to 11 v / v%, for example, 0.01 to 10, 0.1 to 10, 0.5 to 10, 1 to 10, 2 to 10, 3 To 10, 4 to 10, 5 to 10, 6 to 10, 7 to 10, 8 to 10, 9 to 10 or 10 v / v%, but is not limited thereto.
  • the lactic acid bacteria culture solution may be sterile.
  • the cosmetic composition containing the lactic acid bacteria culture solution may be used for skin whitening, but is not limited thereto.
  • the cosmetic composition may be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils It may be formulated as powder foundation, emulsion foundation, wax foundation, spray, and the like, but is not limited thereto.
  • it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.
  • the carrier components include animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. Can be used.
  • the cosmetic formulation of the present invention is a powder or a spray
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular, in the case of a spray, additionally chlorofluorohydrocarbon.
  • Propellant such as propane / butane or dimethyl ether.
  • a solvent, solubilizer or emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.
  • the cosmetic formulation of the present invention is a suspension
  • a liquid diluent such as water, ethanol or propylene glycol
  • suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
  • the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
  • the components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions, in addition to the lactic acid bacteria culture liquid mixture and carrier components as active ingredients, for example, antioxidants, stabilizers, solubilizers, vitamins, pigments And conventional adjuvants such as fragrances.
  • Another aspect of the present invention relates to a method for preparing a lactic acid bacteria culture mixture comprising the following steps.
  • the lactic acid bacteria may be at least two selected from the group consisting of Lactobacillus rhamnosus, Lactobacillus ashidophyllus and Bifidobacterium animalis, for example, Lactobacillus rhamnosus, Lactobacillus ashidophyllus and Bifidobacterium
  • Therium animalis may be, but is not limited thereto.
  • Preparing two or more kinds of the lactic acid bacteria culture may include the following steps.
  • the lactic acid bacteria may be freeze-dried, but is not limited thereto.
  • the medium may be MRS broth medium, but is not limited thereto.
  • the medium may be a liquid medium, but is not limited thereto.
  • the liquid medium is sterilized at an autoclave, for example, at 121 ° C. for 15 minutes, and then at 15 to 30 ° C., for example, 20 to 30, 25 to 30, 25 to 28, 26 to 28 or It can be cooled and used at 27 degreeC.
  • the culture may be cultured by standing anaerobic.
  • the culture temperature may be 30 to 40 °C, for example, 31 to 40, 32 to 40, 33 to 40, 34 to 40, 35 to 40, 36 to 39, 36 to 38, 36 to 37 or 37 °C may be, but is not limited thereto.
  • the incubation time of the step of obtaining the first culture may be 36 hours, but is not limited thereto.
  • the incubation time of the step of obtaining the second culture may be 48 hours, but is not limited thereto.
  • the centrifugal pressure may be 4500 rpm, but is not limited thereto.
  • the centrifugation time may be 1 to 1.5 hours or 1 hour, but is not limited thereto.
  • the sterilization may be performed by filtering the supernatant.
  • the filtering may be to filter with a filter of 0.1 to 0.2 ⁇ m, for example 0.2 ⁇ m diameter.
  • Cells may not be separated when a filter having a diameter smaller than the diameter range is used, and active ingredients may be separated together with the cells when a filter having a diameter larger than the diameter range is used.
  • the mixing of the prepared lactic acid bacteria cultures may be performed by mixing two kinds of lactic acid bacteria cultures with a volume of 1: 0.2-5, for example, 1: 0.2-4, 1: 0.2-3, 1: 0.2-2, 1: 0.2 to 1.8, 1: 0.2 to 1.6, 1: 0.2 to 1.4, 1: 0.2 to 1.2, 1: 0.4 to 1.2, 1: 0.6 to 1.2, 1: 0.8 to 1.2 or 1: 1 There is;
  • the three lactic acid bacteria cultures were prepared in volumes of 1: 0.2-5: 0.2-5, for example 1: 0.2-4: 0.2-4, 1: 0.2-3: 0.2-3, 1: 0.2-2: 0.2 To 2, 1: 0.2 to 1.8: 0.2 to 1.8, 1: 0.2 to 1.6: 0.2 to 1.6, 1: 0.2 to 1.4: 0.2 to 1.4, 1: 0.2 to 1.2: 0.2 to 1.2, 1: 0.4 to 1.2: 0.4 To 1.2, 1: 0.6 to 1.2: 0.6 to 1.2, 1:
  • the step of mixing two or more of the lactic acid bacteria culture is a volume of 1: 0.2 to 5: 0.2 to 5 Lactobacillus rhamnosus culture, Lactobacillus ashidophyllus culture and Bifidobacterium animalis culture It may be to mix.
  • Another aspect of the invention relates to a skin whitening method comprising the step of contacting a lactic acid bacteria culture mixture to a subject.
  • the subject may be a mammal including a primate including a human, a monkey, and the like, a rodent including a mouse, a rat, and the like, but is not limited thereto.
  • the contact means direct contact of the lactic acid bacteria culture mixture with or near the site on the subject's body, for example, application of the lactic acid bacteria culture mixture to the human skin.
  • the overlapping contents of the lactic acid bacteria culture mixture is omitted in consideration of the complexity of the present specification.
  • Another aspect of the invention relates to the use of skin whitening of a composition comprising a lactic acid bacteria culture mixture.
  • Another aspect of the present invention relates to a skin whitening method using a lactic acid bacteria culture liquid mixture.
  • the present invention relates to a cosmetic composition containing a culture solution of lactic acid bacteria, a method for preparing the same, a use for the skin whitening thereof, a method for skin whitening using the same, and specifically, Lactobacillus rhamnosus culture, Lactobacillus ashdophyllus culture and
  • the present invention relates to a cosmetic composition for skin whitening containing a lactic acid bacteria culture medium mixed with Bifidobacterium animalis culture, and has excellent melanin production, migration, and secretion inhibitory effects, and thus may be usefully used as a cosmetic composition for skin whitening. .
  • 1 is a graph showing the inhibitory effect of intracellular melanin biosynthesis of the lactic acid bacteria culture solution according to an embodiment of the present invention.
  • Figure 2 is a graph showing the inhibitory effect of extracellular melanin migration and secretion of the lactic acid bacteria culture mixture according to an embodiment of the present invention.
  • Figure 3 is a graph showing the effect of reducing the MITF gene expression of the lactic acid bacteria culture mixture according to an embodiment of the present invention.
  • Lactobacillus for preparing the composition of the present invention was cultured by itself.
  • a fermentation product containing rice (Icheon king rice, Nonghyup, Korea) and Nuruk (acid yeast, Nonghyup, Korea) was prepared, from which Lactobacillus rhamnosus and Lactobacillus ashdophyllus were cultured.
  • Bifidobacterium animalis was isolated and cultured from feces of newborns less than 1 month of age.
  • the cultured spawn cells were mixed with skim milk and dispersed, dried and stored in ampoules, or 2) the culture solution contained 30% glycerin and frozen at -70 ° C.
  • the base sequence of the Lactobacillus rhamnosus is shown in SEQ ID NO: 1, the base sequence of Lactobacillus ashidophilus in SEQ ID NO: 2, Bifidobacterium animalis in SEQ ID NO: 3, respectively. All of the lactic acid bacteria have more than 99% genetic sequence similarity with the standard bacteria, but has a slightly different phenotype.
  • MRS broth MRS broth, Bacto-Difco
  • water water were mixed in a flask to prepare a liquid MRS broth medium having a concentration of 55 g / L.
  • the prepared liquid MRS broth medium was sterilized at 121 ° C. for 15 minutes and cooled to 27 ° C.
  • each liquid MRS broth medium was inoculated with 100 ⁇ L of Bifidobacterium animalis, Lactobacillus rhamnosus and Lactobacillus ashidophilus of Preparation Example 1, followed by anaerobic treatment at 37 ° C. for 36 hours.
  • the culture was allowed to stand.
  • the entire culture was inoculated again in 1 L of liquid MRS broth medium and further incubated at 37 ° C. for 48 hours. After the incubation was completed, each culture was centrifuged at 4500 rpm for 1 hour to remove the cells. Cultures from which the cells were removed were sterilized by filtering with a 0.2 ⁇ m diameter filter (Bottle filter kit, Bacto-Difco).
  • Lactobacillus ashidophilus culture of which filtering of Preparation Example 2 was completed was used in the experiments performed below.
  • the filtered Bifidobacterium animalis culture, the Lactobacillus rhamnosus culture, and the Lactobacillus ashidophyllus culture of the above-prepared example were mixed at a volume ratio of 1: 1: 1 to prepare a lactic acid bacteria culture mixture.
  • Murine pigment cells B16-F10 (Murine Melanoma) cells (ATCC) were suspended in 2 ml of DMEM (HYCLONE) containing 10% FBS and inoculated into 6-well plates at 1 ⁇ 10 5 cells per well, after which the cells bottomed. Incubated for 24 hours at 37 °C, 5% CO 2, until attached to 40-50%.
  • DMEM fetal calf serum
  • ⁇ -MSH was treated at 0.1 ⁇ M concentration, and each sample was added at each concentration (0.1 v / v%, 1 v / v% and 10 v / v%), and then incubated at 37 ° C. for 72 hours. Melanin production was induced. After further incubation, the cells were washed with phosphate buffered saline (PBS, WELGENE) and recovered with trypsin (Gibco).
  • PBS phosphate buffered saline
  • WELGENE phosphate buffered saline
  • hematocytometer (MARIENFELD) was counted, and the same number of cells (1 ⁇ 10 6 cells / mL) was collected for each treatment group, and centrifuged at 1000 rpm for 10 minutes to obtain only cells.
  • the recovered cells were dried at 60 ° C. for 1 hour, and then 100 ⁇ L of 1 M NaOH solution (Sigma aldrich) containing 10% DMSO was added to obtain a solution containing melanin in the cells. This solution was measured with absorbance three times at 490 nm with a Microplate reader (victor3) to evaluate the inhibition of melanogenesis.
  • Evaluation formula is the same as the following formula 1, the results are shown in Table 1 below.
  • Arbutin 100 ppm, Sigma aldrich
  • the lactic acid bacteria cultures of Comparative Examples 1 to 3 did not show a melanin inhibitory effect when treated with a concentration of 1 v / v%, but a melanin production inhibitory effect when treated with a concentration of 10 v / v% Showed.
  • the lactic acid bacteria culture solution of the embodiment shows melanin inhibitory effect from the treatment of 1 v / v% concentration, especially when treated with a concentration of 10 v / v% 7-25% better melanin production than the lactic acid bacteria cultures It confirmed the effect.
  • Murine pigment cells B16-F10 (Murine Melanoma) cells (ATCC) were suspended in 2 ml of DMEM (HYCLONE) containing 10% FBS and inoculated into 6-well plates at 1 ⁇ 10 5 cells per well, after which the cells bottomed. Incubated for 24 hours at 37 °C, 5% CO 2, until attached to 40-50%.
  • DMEM fetal calf serum
  • Murine pigment cells B16-F10 (Murine Melanoma) cells (ATCC), were suspended in 2 ml of DMEM (HYCLONE) containing 10% FBS and inoculated into 6-well plates at 5 ⁇ 10 5 cells per well, after which the cells bottomed. It was incubated for 24 hours at 5% CO 2, 37 °C until attached to at least 80%.
  • DMEM fetal calf serum
  • ⁇ -MSH was treated at a concentration of 0.1 ⁇ M and each sample was added at a concentration of 1 v / v%, followed by further incubation at 5% CO 2, 37 ° C for 24 hours to induce melanin production. After further incubation, it was washed with phosphate buffered saline (PBS, WELGENE) and recovered with 1 mL Trizol (Trizol, TAKARA). Then, 0.2 mL chloroform (Chloroform, SAMCHUN) corresponding to a 1/5 ratio of trizol was added thereto, stirred, and centrifuged at 14000 rpm for 15 minutes at 4 ° C to obtain a supernatant. 0.5 mL isopropanol (isopropanol, DUKSAN) was added to the same supernatant, and reacted for 10 minutes, followed by centrifugation at 14000 rpm for 15 minutes to obtain a precipitate.
  • PBS phosphate buffered saline
  • the cosmetic composition of the present invention by using a mixture of lactic acid bacteria cultures, even when using a small amount of each strain exhibits a similar level of whitening or more than when using a large amount of a single strain, for skin whitening purposes It can be seen that it can be usefully used.
  • the present invention relates to a cosmetic composition containing a culture solution of lactic acid bacteria, a method for preparing the same, a use for the skin whitening thereof, a method for skin whitening using the same, and specifically, Lactobacillus rhamnosus culture, Lactobacillus ashdophyllus culture and
  • the present invention relates to a cosmetic composition for skin whitening containing a lactic acid bacteria culture medium mixed with Bifidobacterium animalis culture, and has excellent melanin production, migration, and secretion inhibitory effects, and thus may be usefully used as a cosmetic composition for skin whitening. .

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Abstract

La présente invention concerne une composition cosmétique contenant une solution de mélange de cultures de lactobacilles, un procédé de préparation de celle-ci, une utilisation de celle-ci pour le blanchiment de la peau, et une méthode de blanchiment de la peau utilisant celle-ci, et, plus particulièrement, une composition cosmétique de blanchiment de la peau contenant une solution de mélange de cultures de lactobacilles dans laquelle sont mélangées une culture de Lactobacillus rhamnosus, une culture de Lactobacillus acidophilus et une culture de Bifidobacterium animalis.
PCT/KR2019/001951 2018-03-09 2019-02-19 Composition cosmétique de blanchiment de la peau contenant une solution de mélange de cultures de lactobacilles Ceased WO2019172554A1 (fr)

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KR1020180027957A KR102026597B1 (ko) 2018-03-09 2018-03-09 유산균 배양물 혼합액을 함유하는 피부 미백용 화장료 조성물
KR10-2018-0027957 2018-03-09

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KR102783644B1 (ko) 2022-02-24 2025-03-19 (주)코엔바이오 유산균 유래 세포밖 소포체를 유효성분으로 포함하는 피부 미백용 조성물
KR102482157B1 (ko) * 2022-07-12 2022-12-29 (주)더마랩 황 함유 아미노산이 함유되어 피부 스트레스 개선 효능이 우수한 락토바실러스 혼합배양액을 유효성분으로 함유하는 화장료 조성물
KR102873992B1 (ko) 2024-01-11 2025-10-21 주식회사 제이투케이바이오 유산균을 이용한 화장료 조성물의 제조 방법 및 이로부터 제조된 유산균 함유 화장료 조성물

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