WO2019062436A1 - Instant inspection device - Google Patents

Abstract

An instant inspection device, comprising a syringe and a plunger. The plunger is movably arranged in the syringe; a blocking object is arranged at a liquid outlet of the syringe, a pre-filling cavity is formed between the blocking object and the plunger, liquid and gas are pre-filled into the pre-filling cavity, and a piercing object is arranged at one end, near the blocking object, of the plungerthe plunger is pushed to compress the gas in the pre-filling cavity, and the piercing object is driven to break or open the blocking object. The quantitative filling of one or multiple component reagents can be realized at one time. the plunger is pushed to compress the gas in the pre-filling cavity; and the piercing object is driven to break or open the blocking object. The quantitative filling of one or multiple component reagents can be realized at one time.

Description

Instant inspection device Technical field

The invention belongs to the field of medical detection, and in particular relates to a real-time inspection device.

Background technique

At present, immunodiagnostic reagents are listed, and the immunoturbidimetric assay is listed. The reagent includes two separate components, enumerating: a detergent having a blood cell lysis function and an agglutinating agent for enhancing the agglutination effect, and enumerating: polyethylene glycol (PEG) and Salts, in addition, there are some reagents consisting of antibodies and preservatives. Usually, when the two components are mixed, the reagents are unstable and need to be mixed before use to achieve the best results. Limited by the above experimental conditions, the on-site detection (POCT) is difficult to apply for immunoturbidimetric reagents; the enumeration of immunoturbidimetric reagents for detecting children requires the collection of peripheral blood, and the reagent detection requires siphon blood collection tube and siphon blood collection tube injection. Rod, reagent 1, reagent 2, reagent cup, two different precision pipettes, pipette tips, and need to be sent to the laboratory for testing after the sample is collected by the clinical department. The entire inspection report time is about 40 minutes, especially It is difficult to popularize in the emergency department and basic hospital, the operation is cumbersome, and it is necessary to transfer samples to different departments, and the human factors are large. The traditional typical reagent detection process: siphon sampling tube collection sample → sample transfer to the inspection department → insert the push rod Siphon sampling tube→Open the reagent cup cover and push the sample into the reagent cup→Adjust the pipette to 300uL→-Install the disposable pipette tip→Open the bottle cap and inject 300 uL reagent 1 into the reaction cup with pipette~Change Pipette tip → adjust the pipette to 100uL → install the disposable pipette tip → open the cap and pipette 100uL reagent 2 into the pipette → after mixing into the cuvette, the entire process takes about 40 minutes.

technical problem

OBJECT OF THE INVENTION In order to overcome the deficiencies in the prior art, the present invention provides a real-time testing device capable of realizing one-time quantitative filling of one or more component reagents, which can make testing more convenient and reagent loading accuracy. Improve, truly check out.

Technical solution

Technical Solution: In order to achieve the above object, a real-time testing device of the present invention comprises a syringe and a plunger, wherein the plunger is movably disposed in the syringe, and a liquid outlet of the syringe is provided with a blockage. a pre-filled cavity is formed between the plug and the plunger, the pre-filled cavity is pre-filled with liquid and gas, and the plunger is provided with a split at one end of the plug; the plunger is pushed, and the plunger is compressed The gas is pre-filled in the chamber, so that the displacement of the plunger drives the splitting material to break or split the plug, and continues to push the plunger, and the pre-filled liquid is injected into the reaction cup.

Further, in the erected state of the syringe, the gas thickness in the pre-filled cavity is a, the distance between the tip end of the pointed column and the blockage is b, a and b satisfy a>b.

Further, in the erected state of the syringe, the gas thickness in the pre-filled cavity is a, the distance between the tip end of the pointed column and the blockage is b, and the gas in the pre-filled cavity is compressed by the plunger The thickness after the c is c, which satisfies ac>b.

Further, the plunger is connected to the prying or the plunger is integrated with the prying material.

Further, the cleavage is a pointed column structure, and a tip end of the pointed column is disposed at a distance from the clogging.

Further, the cross section of the pointed column is a cross shape, and the pointed column is gradually thickened from the plunger end to the tip end.

Further, a push rod is disposed on the opposite side of the splitting of the plunger, and the push rod is provided with a push handle.

Further, a single or a plurality of the syringes form an injection unit; wherein an injection unit composed of a plurality of the syringes includes at least a first syringe and a second syringe; the first syringe and the second injection The cartridges are juxtaposed or connected to each other in parallel; wherein each of the syringes is not connected to each other, and the push handles of the syringes are connected to each other integrally or independently of each other.

Further, a reaction cup is further included, and a liquid discharge end of the injection unit is disposed corresponding to the liquid inlet end of the reaction cup; and a sampler is further included, and the sampling end of the sampler protrudes into the reaction cup.

Further, the reaction cup is detachably connected to the injection unit; the bottom of the cup of the reaction cup is electrically connected with a detection window, and the detection window is a transparent container having a cross-sectional area smaller than a cross-sectional area of the reaction cup.

Further, the inner wall of the syringe is provided with at least one ring-shaped positioning ring, and the tip of the pointed column has a spacing between the tip and the blockage when the plunger is at the positioning ring.

Further, the pre-filled cavity is filled with powdered solids and gas, or the pre-filled cavity is filled with a blocky solid and a gas.

Beneficial effect

The invention has the advantages that the structure of the invention is simple, the quantitative filling of one or more component reagents can be realized at one time, the inspection can be more convenient, the precision of the reagent sample loading can be improved, and the real-time inspection can be realized; The reagent detection step is reduced to three steps, and the traditional detection time of more than 40 minutes is shortened to about 2 minutes. If the scheme can be effectively promoted, the operation efficiency in the medical detection field can be greatly improved.

DRAWINGS

1 is a schematic view of the overall structure of the present invention; FIG. 2 is a schematic view of the structure of the two syringes; FIG. 3 is a schematic view of the structure of the plunger, the push rod and the pointed column; FIG. 4 is a schematic view of the structure of the reaction cup; Schematic diagram of the syringe structure.

Embodiments of the invention

In the present embodiment, the invention is applied to the field of medical sample detection, and the present invention will be further described below with reference to the accompanying drawings.

As shown in FIGS. 1 to 4, the present embodiment includes an injection unit and a reaction cup 7, the liquid discharge end of the injection unit is disposed corresponding to the liquid inlet end of the reaction cup 7, and the reagent in the injection unit can flow to the reaction cup. 7; further comprising a sampler 11 connected to the liquid discharge end of the injection unit, and the reaction cup 7 is detachably connected to the injection unit, in this embodiment: the injection unit The liquid outer ring is provided with a sealing ring groove 16, and the cup end 10 of the upper end of the reaction cup 7 is sleeved on the sealing ring groove 16, which is beneficial for disassembly and effectively prevents the reagent in the reaction cup 7 from splashing; the reaction cup 7 and In the assembled state of the injection unit, the sampling end 8 of the sampler 11 extends into the cuvette 7, facilitating the reaction of the collected sample in the reaction cup 7, and after the reaction cup 7 is removed, the sampler 11 follows the injection unit. The sampling and installation of the sampler 11 is facilitated.

The cup bottom of the cuvette 7 is electrically connected to the detection window 9, and the detection window 9 is a transparent container having a cross-sectional area smaller than the cross-sectional area of the cuvette 7. In this embodiment, the reaction cup is also a machine glass or a high transparent plastic. The transparent body, and the detection window 9 and the reaction cup 7 form a conjoined structure; the detection window 9 can intuitively judge the mixing degree of the reagent and the sample and preliminarily judge the reaction effect, for example, when the sample is mixed with the reagent 1 and the reagent 2 After the reaction, the specific analyte undergoes a specific reaction, and the agglutination increases the turbidity of the solution. The optical instrument can change the scattering of the light after detecting the window 9. The change of the light scattering can be measured by the instrument, and the amount of light scattering is in the sample. The concentration of the analyte is proportional.

The injection unit includes a single syringe or syringe set; the syringe set includes at least a first syringe 14 and a second syringe 13; the first syringe 14 and the second syringe 13 are juxtaposed; The plunger 3 is disposed in the syringe, wherein each of the syringes is not connected to each other; in the embodiment, the material of the plunger 3 is preferably a material such as silica gel, and the plunger 3 and the inner wall of the syringe are interspersed for improving the sealing performance. Cooperating, the outlet of the syringe is provided with a blockage 6, and the blockage 6 blocks the outlet. The blockage 6 can be increased or decreased according to the diameter of the outlet port. The material of the plug hole is not particularly limited, but must be blocked. The hole can be opened or smashed by the pointed column, and the outlet can be increased or decreased according to the need. In the present embodiment, the typical plug 6 is listed as a sealing plug of a plastic, glass, resin or the like, a sealing ball, or the like, or a heat seal. a plastic film, aluminum foil or the like sealed in a manner such as a glue bond; a pre-filled cavity 30 is formed between the plug 6 and the plunger 3, and a liquid and a gas are pre-filled in the pre-filled cavity 30, the plunger 3 is provided near the end of the blockage 6 Pushing the plunger 3, the plunger 3 compresses the gas in the pre-filled cavity 30, so that the displacement of the plunger 3 drives the splitting material to break or split the plugged object 6, and continues to push the plunger 3 to pre-fill the liquid Injecting the reaction cup; in the embodiment, the plunger 3 is connected to the prying material, or the plunger 3 is integrated with the cleaving object; the gas in the embodiment is a chemically stable gas such as air, nitrogen or an inert gas. It is guaranteed that the gas does not react with the liquid reagent.

Each of the plungers 3 is provided with a push rod 2 on the opposite side of the splitting body, and the push rod 2 is provided with a push handle 1; the push handles 1 of each of the injection cylinders are connected to each other or are independent of each other, and a plurality of specific types In the case of a reagent, it is necessary to supply the reaction cup 7 in a sequential order, and the push handle 1 independent of each other can fulfill this requirement.

The splitting is a structure of a pointed column 4, the tip end 5 of the pointed column 4 is spaced from the blockage 6; the cross section of the pointed column 4 is a cross, and the pointed column 4 is The end of the plunger 3 is gradually thickened to the tip end 5, and a cross-shaped section and a gradually thickened structure facilitate the flow of the reagent into the reaction cup.

The pre-filled chamber 30 is filled with liquid and gas or filled with powder solids and gas at the same time or filled with bulk solids and gas; in this embodiment, the pre-filled chamber 30 is filled with reagents and gases at the same time; In the erected state of the syringe, the gas thickness in the pre-filled cavity 30 is a, the distance between the tip end 5 of the pointed post 4 and the plug 6 is b, a and b satisfy a>b due to the material and The thrust limitation, the gas compression ratio cannot be increased indefinitely, so the thickness of the gas in the pre-filled cavity 30 after being compressed by the plunger 3 is c, and should also satisfy ac>b, which ensures the tip end 5 of the pointed column 4 The stroke can reach the blockage 6.

The inner wall of the syringe is provided with at least one ring-shaped positioning ring 15 with a spacing between the tip end 5 of the pointed column 4 and the blockage 6 when the plunger 3 is at the positioning ring 15; The cross-section of the inner wall of the syringe is circular, elliptical or polygonal; the positioning ring 15 acts as a safety clasp for positioning and locking the initial position of the plunger 3 and the push rod 2.

The sampler 11 is a quantitative capillary blood collection tube or a quantitative blood collection rod; the capillary end on the quantitative capillary blood collection tube is a sampling end 8 , and the fixed column end on the quantitative capillary blood collection tube is a connection end; sampling on the sampling rod The ring end is the sampling end 8 and the other end is the connecting end; wherein the sampling circle is a circular or elliptical sampling structure, and the sampling circle is notched or notched; the quantitative capillary collecting tube or the quantitative blood collecting rod is connected at the connecting end In the mounting holes 12 between the two outlet ports of the first syringe 14 and the second syringe 13.

The method description of the device specifically includes the following steps: Step 1, the reaction cup 7 is detached from the injection unit, the sampler 11 collects the sample, and the reaction cup 7 is reinstalled; Step 2, simultaneously or stepwise pressing the first syringe 14 And the push rod 2 on the second syringe 13, the gas in the pre-filled chamber 30 in the first syringe 14 and the second syringe 13 is compressed until the two pointed columns 4 puncture the two outlets respectively. The plug 6, the first reagent in the first syringe 14 and the second reagent in the second syringe 13 are respectively ejected from the two outlets under the action of the compressed gas, and flow into the reaction cup 7; After mixing the reaction cup 7, the detection window 9 is observed and placed in the instrument for detection.

Supplementary explanation: when a plurality of conjoined syringes are simultaneously bolused, the distance between the tip end 5 of the pointed column 4 and the blockage 6 may be increased or decreased according to the amount of liquid in the pre-filled cavity 30, or the pre-filled cavity 30 may be controlled. The amount of gas, so as to achieve the order of filling the liquid as needed; in the same pre-filled chamber 30, the more gas, the less the liquid, the farther the tip 5 of the pointed column 4 can be from the blockage; in certain cases, The reagent in the pre-filled chamber 30 can be applied to a part of the solid, the solid requirement is smaller than the inner diameter of the liquid outlet, the sharp shape 4 pushes the solid, the solid smashes or smashes the plug, and the solid comes out of the plug.

The above description is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can also make several improvements and retouchings without departing from the principles of the present invention. It should be considered as the scope of protection of the present invention.

Claims (12)

A real-time testing device, comprising: a syringe barrel and a plunger (3), wherein the plunger (3) is movably disposed in the syringe barrel, and a liquid outlet of the syringe barrel is provided with a blockage (6) a pre-filled cavity (30) is formed between the plug (6) and the plunger (3), the pre-filled cavity (30) is pre-filled with liquid and gas, and the plunger (3) is adjacent to the One end of the blockage (6) is provided with a clam; the plunger (3) is pushed, and the plunger (3) compresses the gas in the pre-filled cavity (30), so that the displacement of the plunger (3) drives the clam The clogging (6) is smashed or split, the plunger (3) is pushed further, and the prefilled liquid is injected into the cuvette. A real-time testing device according to claim 1, characterized in that, in the erected state of the syringe, the gas thickness in the pre-filled cavity (30) is a, the tip of the pointed column (4) ( 5) The distance from the plug (6) is b, a and b satisfy a>b. A real-time testing device according to claim 1, characterized in that, in the erected state of the syringe, the gas thickness in the pre-filled cavity (30) is a, the tip of the pointed column (4) ( 5) The distance from the plug (6) is b, and the thickness of the gas in the pre-filled chamber (30) after being compressed by the plunger (3) is c, which satisfies ac>b. A real-time testing device according to claim 1, characterized in that the plunger (3) is connected to the prying material or the plunger (3) is integrated with the prying material. A point-in-time inspection device according to claim 1, characterized in that the crotch is a pointed column (4) structure, the tip end (5) of the pointed column (4) and the obstruction ( 6) Spacing settings. A real-time inspection device according to claim 5, characterized in that the cross section of the pointed column (4) is a cross, and the pointed column (4) is from the end of the plunger (3) to the tip end ( 5) Gradually thicker. A real-time inspection device according to claim 1, characterized in that the opposite side of the plunger (3) is provided with a push rod (2), and the push rod (2) is provided with a push handle (1). A point-in-time test apparatus according to claims 1 and 7, wherein a single or a plurality of said syringes form an injection unit; wherein an injection unit composed of a plurality of said syringes comprises at least a first syringe (14) and a second syringe (13); the first syringe (14) and the second syringe (13) are juxtaposed or connected side by side; wherein each of the syringes is not connected to each other, each of the The push handles (1) of the syringe are connected to each other or to each other. A real-time testing device according to claim 8, further comprising a reaction cup (7), the liquid discharge end of the injection unit is corresponding to the liquid inlet end of the reaction cup (7); A sampler (11), the sampling end (8) of the sampler (11) projects into the cuvette (7). A real-time testing device according to claim 9, characterized in that the reaction cup (7) is detachably connected to the injection unit; the bottom of the cup of the reaction cup (7) is provided with a detection window ( 9) The detection window (9) is a transparent container having a cross-sectional area smaller than a cross-sectional area of the cuvette (7). A real-time testing device according to claim 1, wherein the inner wall of the syringe is provided with at least one ring-shaped positioning ring, and the plunger (3) is located at the positioning ring (15). There is a gap between the tip end (5) of the pointed column (4) and the plug (6). A point-in-time inspection apparatus according to claim 1, wherein: said pre-filled chamber (30) is filled with powdered solids and gas, or said pre-filled chamber (30) is filled with bulk solids and gas. .