[go: up one dir, main page]

WO2018110739A1 - Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries - Google Patents

Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries Download PDF

Info

Publication number
WO2018110739A1
WO2018110739A1 PCT/KR2016/014707 KR2016014707W WO2018110739A1 WO 2018110739 A1 WO2018110739 A1 WO 2018110739A1 KR 2016014707 W KR2016014707 W KR 2016014707W WO 2018110739 A1 WO2018110739 A1 WO 2018110739A1
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria
coating material
slime
adsorbent
rhodobacter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2016/014707
Other languages
English (en)
Korean (ko)
Inventor
양근혁
윤현섭
이광명
이상섭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sungkyunkwan University
Industry Academic Cooperation Foundation of Kyonggi University
Original Assignee
Sungkyunkwan University
Industry Academic Cooperation Foundation of Kyonggi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sungkyunkwan University, Industry Academic Cooperation Foundation of Kyonggi University filed Critical Sungkyunkwan University
Priority to PCT/KR2016/014707 priority Critical patent/WO2018110739A1/fr
Publication of WO2018110739A1 publication Critical patent/WO2018110739A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B41/00—After-treatment of mortars, concrete, artificial stone or ceramics; Treatment of natural stone
    • C04B41/45—Coating or impregnating, e.g. injection in masonry, partial coating of green or fired ceramics, organic coating compositions for adhering together two concrete elements
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D1/00—Coating compositions, e.g. paints, varnishes or lacquers, based on inorganic substances
    • C09D1/06—Coating compositions, e.g. paints, varnishes or lacquers, based on inorganic substances cement
    • C09D1/08—Coating compositions, e.g. paints, varnishes or lacquers, based on inorganic substances cement with organic additives
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/08—Anti-corrosive paints
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
    • C09D5/16—Antifouling paints; Underwater paints
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • E—FIXED CONSTRUCTIONS
    • E03—WATER SUPPLY; SEWERAGE
    • E03F—SEWERS; CESSPOOLS
    • E03F3/00—Sewer pipe-line systems
    • E03F3/06—Methods of, or installations for, laying sewer pipes

Definitions

  • the present invention relates to concrete protective material technology, and more particularly to a bacterial slime-based concrete protective coating.
  • the present invention is derived from the research carried out by the grant support of the Ministry of Land, Infrastructure and Transport construction technology research project.
  • the present invention proposes a coating material using slime (glyco callis membrane) bacteria as a basic technology for fusing bacteria and concrete technology, and based on this, to evaluate the effect of bacterial slime-based coating on the sulfuric acid behavior of concrete.
  • an object of the present invention is to select an optimal bacterium capable of slime formation, to cultivate bacteria for slime formation and to present an optimal medium condition, and to present an optimal adsorbent for immobilization of slime-formed bacteria.
  • the present invention provides a coating material comprising an adsorbent and a binder adsorbed slime forming bacteria.
  • the present invention for solving the another problem, the step of culturing the slime forming bacteria to form a slime; Adsorbing the slime-formed bacteria using an adsorbent to fix the slime-formed bacteria; And mixing the bacteria adsorbed material with the binder.
  • Rhodobacter Rhodobacter capsulatus Rhodobacter Blind stitch kusu (Rhodobacter blasticus), Rhodobacter sphaeroides (Rhodobacter sphaeroides ), Rhodopseudomonas photo showing the formation of a slime (glycocalyx) membrane around cells in palustris ) and Rubrivivax gelatinosus ,
  • FIG. 2 is a schematic diagram showing a performance degradation mechanism of concrete by sulfuric acid
  • Figure 3 is a schematic diagram showing the bacteria-based coating mechanism of the slime and SEM analysis of the coating microstructure
  • Figure 4 is a schematic diagram showing an exemplary adsorption pad (a) and the culture vessel (b) in which the adsorption pad is immersed in the embodiment of the present invention
  • Figure 5 is a photograph showing the changed state after 7 days of cultured bacteria in the embodiment of the present invention.
  • Figure 6 is a micrograph of the shape and structure of the slime membrane of Rhodobacter capsulatus cultured in an embodiment of the present invention
  • Figure 7 is a photograph showing the freeze-dried slime membrane according to each medium in the embodiment of the present invention.
  • Figure 8 is also in connection with an embodiment of the present invention bakteo la capsule tooth (Rhodobacter capsulatus ) and graph showing slime production,
  • FIG. 10 is a photograph showing the surface structure of a high absorbent polymer in an embodiment of the present invention.
  • Figure 11 is a photograph showing the surface structure of a highly porous resin in an embodiment of the present invention.
  • Figure 13 is a photograph showing the surface structure of pearlite in the embodiment of the present invention.
  • FIG. 17 is a schematic diagram and photographs illustrating a method for measuring sulfuric acid penetration depth of concrete immersed in a 5% sulfuric acid solution in Experimental Example 1 of the present invention
  • 26 and 27 are graphs showing the results of analyzing the main diffraction peaks of the reaction product of the sample taken from the first group of concrete surface in Experimental Example 1 of the present invention.
  • 41 is a graph showing a weight change of sulfuric acid immersion days of the second group in Experimental Example 1 of the present invention.
  • 43 and 44 are graphs each showing the ratio of decrease in dynamic modulus of elasticity at the center of the test body and the ratio of decrease in dynamic modulus of the surface at 28 days of immersion of sulfuric acid in the second group in Experimental Example 1 of the present invention;
  • 54 and 55 are graphs showing the dynamic modulus reduction ratio and the dynamic modulus reduction ratio of the central portion of the test specimen at 28 days of immersion of sulfuric acid of the third group in Experimental Example 1 of the present invention, respectively;
  • 59 and 60 are graphs showing the dynamic modulus reduction ratio of the central portion and the dynamic modulus reduction ratio of the central portion of the test body at 28 days of sulfuric acid immersion in comparison with the conventional technology in Experimental Example 1 of the present invention, respectively;
  • Figure 61 is a photograph showing the appearance of the specimens by immersion days according to the sulfuric acid immersion in Experimental Example 2 of the present invention.
  • FIG. 64 is a photograph showing a microstructure analysis result for evaluation of adsorption of bacteria after 28 days of immersion in a 5% sulfuric acid aqueous solution in Experimental Example 2 of the present invention.
  • 65 is a graph showing a change in mass of a test specimen according to an immersion age in Experimental Example 3 of the present invention.
  • 66 is a graph showing a change in compressive strength of a test specimen according to immersion age in Experimental Example 3 of the present invention.
  • 67 is a photograph showing the results of confirming the formation of a colony by re-inoculating (passaging) a sample of the surface of the coating material collected after 7 days of dipping age in Experimental Example 4 of the present invention
  • 69 is a graph showing the compressive strength and pH measurement results according to the type of phosphate in Experimental Example 4 of the present invention.
  • the present invention discloses a coating material comprising an adsorbent and a binder for adsorbing slime forming bacteria, the coating material comprising the steps of culturing the slime forming bacteria to form a slime; Adsorbing the slime-formed bacteria using an adsorbent to fix the slime-formed bacteria; And mixing the bacteria adsorbed material with the binder.
  • Rhodobacter la capsule tooth Rhodobacter capsulatus
  • Rhodobacter Blind stitch kusu Rhodobacter blasticus , Rhodobacter sphaeroides , Rhodopseudomonas palustris , Rubrivivax gelatinosus
  • purple sulfur bacteria green sulfur bacteria, Rhodoseudomonas palustris , Bacillus thuringiensis , Bacillus subtilis , etc. May be used).
  • Rhodobacter capsulatus is able to grow under aerobic and anaerobic conditions. Jeonghwahwa (2010) used Rhodobacter capsulatus to remove nitrogen and phosphorus from sewage and wastewater treatment facilities.
  • Rhodobacter capsulatus Rhodobacter capsulatus
  • Rhodobacter blasticus Rhodobacter blasticus
  • Rhodobacter sphaeroides Rhodopseudomonas palustris
  • Rubrivivax gelatinosus shows the formation of a slime (glycocalyx) membrane around the cell.
  • the coating material according to the present invention can be used to prevent chemical erosion of the surface of the concrete structure, hereinafter will be described as an example of the degradation mechanism of the concrete by sulfuric acid and the slime bacteria-based coating mechanism.
  • Figure 2 is a schematic diagram showing the performance degradation mechanism of concrete by sulfuric acid.
  • the corrosion stage of the concrete exposed to the sulfuric acid environment can be largely divided into three stages.
  • sulfate ions SO 4 2-
  • SRB sulfur reduction bacteria
  • H 2 S hydrogen sulfide
  • SRB sulfur reduction bacteria
  • the produced sulfuric acid reacts with calcium hydroxide (Ca (OH) 2 ), which is a cement component, to produce dihydrate gypsum (CaSO 4 ⁇ 2H 2 O) (see Scheme 2 below).
  • step 3 the resulting dihydrate gypsum and tricalcium aluminate (C3A) react to produce ettringite (see Scheme 3 below).
  • Ettringite and Gypsum produced by reaction with cement hydrate generate expansion and softening of concrete and breakage and cracking of tissue structure.
  • 3 is a schematic diagram showing the bacteria-based coating mechanism of the slime and SEM analysis of the coating microstructure.
  • slime forming bacteria Rhodobater capsulatus
  • the nature of these bacteria attracts various ions (elements such as silicon, magnesium and calcium dissolved in water) including calcium from the surrounding environment.
  • the silica component (SiO 2 ) and the small amount of calcium carbonate (CaCO 3 ) formed inside the coating material are minerals synthesized by combining inorganic and organic materials, and the precipitation reaction of the biochemical concept accompanied by the microbial metabolism action, not the precipitation of the pure chemical concept.
  • the slime film thus formed makes the internal structure of the coating material dense and low permeability, thereby preventing harmful substances such as sulfate from invading from the outside. Furthermore, even if sulfate penetrates into the coating material, organic-inorganic minerals such as silica (SiO 2 ) formed inside the coating material fill the internal pores with fine particles, and as the age increases, the internal structure becomes dense and durable due to the pozzolanic reaction. Effective in promoting Therefore, the species specificity of the bacteria and the medium (enzyme) determine the characteristics of the mineral crystal, and it is judged to give the species specificity and the medium (enzyme) effect on the durability and density of the coating material.
  • silica SiO 2
  • hydrogen sulfide is used as an electron donor to decompose hydrogen sulfide under anaerobic or through anaerobic conditions, which is more advantageously employed.
  • Can be, Rhodobater capsulatus is a bacterium used to remove nitrogen and phosphorus from sewage treatment plants, which can be applied to slime bacteria-based coatings to self-cleaning.
  • the present invention proposes an optimal medium composition and culture conditions having excellent efficiency in inoculation culture based on the characteristics of each bacterium based on the results of research on a special medium composition to create an optimal culture environment of the bacteria.
  • Rhodobacter capsuleratus Rhodobacter capsulatus
  • Rhodobacter blasticus Rhodobacter blasticus
  • Rhodobacter sphaeroides Rhodopseudomonas palustris
  • Rubrivivax gelatinosus purple sulfur bacteria and green sulfur bacteria
  • yeast extract 1 ⁇ 50g of disodium succinate hexahydrate
  • ethanol anhydride Absolute ethanol
  • iron citrate solution Feric citrate solution
  • KH 2 PO 4 potassium dihydrogen phosphate
  • MgSO 4 .7H 2 O magnesium sulfate heptahydrate
  • Bacillus thuringiensis Bacillus thuringiensis
  • Bacillus subtilis Bacillus subtilis 1 to 10g of peptic digest of animal tissue (Peptic digest of animal tissue), yeast extract (Yeast extract) 0.5 ⁇ 3g
  • It is preferably incubated at a pH of 4 to 10 in a medium containing 1 to 10 g of sodium chloride and 0.5 to 3 g of beef extract, and 3 to 7 g of peptic digest of animal tissue. More preferably, cultured at a pH of 6 to 8 in a medium containing yeast extract (Yeast extract) 1-2g, sodium chloride (3-7g) and beef extract (Beef extract) 1-2g.
  • maltose Maltose
  • Dextrose dextrose
  • Fructose fructose
  • Maltose a ratio of 0.1 to 1% by weight of the medium
  • the growth rate and slime production of bacteria preferably maltose (Maltose) may be used in a 0.2 to 0.5% ratio.
  • a material for adsorption of slime-forming bacteria in the present invention a material having excellent porosity cation exchange ability may be used.
  • a high absorbent resin, a high porous resin, expanded vermiculite, pearlite, diatomaceous earth, and the like may be used.
  • the present invention has a porous structure (with an effective water content of 40% by volume and a porosity of 50% or more) by a myriad of pores, thereby providing a material having excellent moisture absorption and moisturizing power. To absorb and use bacteria.
  • the bacterial adsorption materials presented in the present invention have the property of adsorbing organic matter by exchange cations (Mg 2 + , Ca 2 +, etc.) present on the surface of the material to absorb the organic nutrients (medium components) necessary for bacteria and bacterial growth. do.
  • the pH is 6-9, which is the ideal material for the optimum environment for bacteria to grow.
  • Immersion process may be used for adsorption of bacteria using the adsorbent.
  • the mixing amount of the slime-formed bacteria used for the adsorption to the adsorbent is 50 when the adsorbent is the high absorbent resin or the high porous resin based on the weight of the bacterial culture. It is preferable that it is -200 times, It is more preferable that it is 100-150 times, When the said adsorption material is said expanded vermiculite, pearlite or diatomaceous earth, it is preferable that it is 5-20 times, and it is more preferable that it is 10-15 times.
  • immersing the adsorbent in the bacterial culture medium is preferably carried out by storage for 1 to 10 days at 40 ⁇ 80% humidity and 5 ⁇ 40 °C conditions, 2 ⁇ under conditions of 50 ⁇ 70% humidity and 10 ⁇ 30 °C temperature after immersion. More preferably, it is stored for 5 days.
  • FIG. 4 exemplarily shows an adsorption pad (a) and a culture vessel (b) in which the adsorption pad is immersed.
  • the adsorption material is introduced and discharged through the open / close clip 110, and when the plurality of adsorption pads 120 are used, the adsorption pads 120 are connected to each other by using the connecting rods 130.
  • the counterweight 140 is connected to the lowermost suction pad 120 so that the pad 120 floats while maintaining a constant interval.
  • FIG. 4 illustrates a threaded lid 150, a threaded opening 160, and an annular pin 170 to prevent contamination by other bacteria.
  • Mesh-type adsorption pads that can contain adsorbents, such as expanded vermiculite, are preferably manufactured using steel such as aluminum.
  • the size of the mesh is determined in consideration of the particle size of the adsorbents used, but may be preferably used. Considering the type of the mesh eye size is 100 ⁇ m ⁇ 5mm and the thickness of the mesh type adsorption pad is used in the range of 0.5 ⁇ 50mm, preferably the size of the mesh eye is 300 ⁇ m ⁇ 3mm and the thickness of the mesh type adsorption pad The range of 1 to 30mm, more preferably the size of the mesh eye is 500 ⁇ m ⁇ 1mm and the thickness of the mesh type adsorption pad can be used in the range of 2 ⁇ 10mm.
  • the length and width of the mesh adsorption pads vary with the diameter of the culture vessel.
  • the mesh type suction pad is provided with an open / close clip to freely input and discharge the absorbent material, and an annular pin is installed for fixing.
  • the mesh type suction pads are connected to each other using steel rods such as aluminum.
  • the bottom layer of the mesh adsorption pad is provided with a counterweight to prevent floating, the weight of the counterweight for the immersion depth design is determined from the following equation (1).
  • Equation 1 d is the immersion depth of the adsorption pad, W L is the loading load, W S is the fixed load, L and B is the length and width of the adsorption pad, ⁇ w is the unit volume weight of the culture.
  • the fixed load (W S ) among the elements for setting the target immersion depth (d) may change according to the volume and specific gravity of the adsorption pad, and accordingly, the load load (W L ) is fluidly changed as a counterweight to target immersion. Depth can be secured.
  • the adsorption of bacteria using the adsorbent is performed by floating the adsorption pad 120 into the culture medium of the bacteria after injecting the adsorption material into the mesh-type adsorption pad 120, and the adsorption material contained in the adsorption pad 120. Can be suspended in the middle of the culture depth and allow for even absorption of bacteria and organic nutrients (medium components).
  • the final coating material is prepared by adsorbing the bacteria using the adsorbent and then mixing the adsorbent with the bacteria adsorbed with the binder.
  • the binder may be an ocher-based binder, ⁇ -half gypsum, blast furnace slag, fly ash, usually portland cement or magnesia-phosphate binder, especially considering the mechanism of chemical erosion of concrete sewer pipe and the sulfuric acid resistance mechanism of slime forming bacteria.
  • ocher-based binders, ⁇ -half gypsum or magnesia-phosphate binders may be used, and most preferably magnesia-phosphate binders may be used, given further bacterial sustainability.
  • the magnesia-phosphate binder exhibits a characteristic that a magnesia-phosphate complex is formed and cured by the reaction of magnesium oxide (MgO) and phosphate (PO 4 ⁇ ).
  • the pH and strength expression properties of the magnesia-phosphate binder are most affected by the mixing ratio of magnesium oxide and phosphate.
  • a large amount of mixed cement (10 to 20% cement) can be expected to reduce the pH in the long term, and can also develop the required compressive strength (adhesive strength). Therefore, it is preferable to use magnesia-phosphate composite as the main binder of the coating material as a binder to maintain the proper pH (8-10) for the continuous growth of bacteria and to secure the required adhesion strength with the concrete structure.
  • the efficiency and adhesion strength of the coating material are determined by the bacterial incorporation and growth rate, and the binder strength and amount, respectively, the mixing ratio of the adsorbent, the binder, and the fine aggregate (if necessary) containing bacteria is a very important factor that determines the performance of the coating material. to be.
  • the condensation time of the binder is one of the most important performance in the coating of sewage pipe should be able to control this. Therefore, the formulation design of coating components for the required performance (flow, compressive strength and sulfuric acid resistance, etc.) is very important.
  • the amount of the binder used when mixing the adsorbent and the binder adsorbed with bacteria is preferably 0.5 to 3 times the weight of the adsorbent adsorbed with bacteria, more preferably 1 to 1.5 times when the binder is an ocher-based binder.
  • the binder is ⁇ -half gypsum, blast furnace slag, fly ash, ordinary portland cement or magnesia-phosphate binder, it is preferable that the binder is 0.5-3 times the weight of the adsorbent adsorbed, and more preferably 1.5-2.5 times.
  • Bacteria adsorbed adsorbents and binders are weighed and mixed in a mixing vessel, and the final coating material is applied to the surface of the concrete structure exposed to chemical erosion and used to prevent chemical erosion.
  • the coating thickness is preferably 0.5 to 10 mm, and more preferably 2 to 4 mm in terms of material economics, with the long-term performance of the coating material.
  • Rhodobacter capsulatus and the basic medium for culturing Rhodoseudomonas palustris are shown in Table 1 below, and the basic medium for culturing Bacillus thuringiensis and Bacillus subtilis . Table 2 shows.
  • Rhodobacter capsulatus based on the medium, the carbon source (dextrose, maltose and frutose) was added 0.3% to the weight of the medium, respectively, the pH was adjusted to 6.8, and then 0.1% (v / v) of Rhodobacter capsularus ( Rhodobacter capsulatus ) was inoculated and cultured according to each carbon source. Inoculated bacteria were incubated using an incubator for 7 days at roughness 2,000 lux, 30 ° C. under anaerobic conditions. 5 shows the changed state after 7 days of cultured bacteria, and the culture state was confirmed by observing the change of the medium color with the naked eye.
  • the carbon source dextrose, maltose and frutose
  • Rhodobacter Rhodobacter capsulatus was examined under the microscope to confirm the shape and structure of the slime membrane. Rhodobacter capsulatus was stained using the Maneval's staining method to distinguish between slime membranes and cells. 6 shows the results of the microscopic observation, and the portion stained in pink is Rhodobacter ( Rhodobacter). capsulatus ) and the white part surrounding the cell is a slime (glycocalyx) membrane.
  • FIG. 7 is a photograph showing the freeze-dried slime membrane for each medium
  • Figure 8 is Rhodobacter ( Rhodobacter capsulatus ) and a graph showing the amount of slime production
  • FIG. 9 is Rhodobacter capsulatus ) is a graph showing the slime composition ratio.
  • Rhodobacter capsulatus showed the fastest growth rate at 0.89 g / L in maltose as shown in FIG. 8.
  • Rhodobacter slime of capsulatus showed the highest yield at about 0.25 g / L.
  • Rhodobacter cultured in maltose Rhodobacter capsulatus
  • Rhodobacter capsulatus cultured in fructose showed the smallest growth rate of 0.3 g / L
  • slime per cell had about 21% of Rhodobacter capsulatus in Rhodobacter. capsulatus ) showed similar yields.
  • the four absorbents evaluated in this example were T's high absorbent resin (Hydrogel), T's high porous resin (Hydrogel), S's expanded vermiculite, and S's pearlite.
  • bacteria are affected by adsorption by the surface structure, specific surface area, and surface hydrophobicity of the material (pederesn, 1990; Kidda et al., 1992).
  • the surface of each adsorbent before adsorption of the bacteria The structure and specific surface area were evaluated. Surface structures were observed using scanning electron microscopy (SEM). Specific surface area was also evaluated using Bruneter, Emmett, Teller (BET).
  • the highly absorbent resin is a three-dimensional network structure made by crosslinking a hydrophilic polymer such as a carboxyl group (COO-) and the like (Hwang, Jun-Seok, 2008).
  • Highly absorbent resins have the property of absorbing and expanding hundreds of times their own weight due to their hydrophilicity. However, it is insoluble in water due to the crosslinking structure (Park Sang-bum, 1994).
  • the surface structure of the super absorbent polymer does not have surface pores, but pores of several hundred micrometers or more are present in a honeycomb shape.
  • the highly absorbent resin is absorbed, adsorbed, and expanded by internal diffusion (Jun Hwang, 2008).
  • the specific surface area of the high water absorbent resin was measured at 0.11 m 2 / g.
  • the highly porous resin has pores of various sizes on the surface through the grinding of the high absorbent resin (Hydrogel) and can be rapidly absorbed, adsorbed, and swelled by capillary action.
  • the highly porous resin has a porous structure in which pores are connected to each other, as shown in FIG. 11.
  • the specific surface area of the high porosity resin was determined to be 3.54 m 2 / g.
  • the expanded vermiculite shows a surface structure in which a layer is formed as a comb layer between layers. It is a structure formed when the vermiculite is heated to 900 ⁇ 1,000 °C, the moisture between the layers turns into steam (Song Jae-hong, 2009). Physical properties and chemical compositions of the expanded vermiculite are shown in Table 3 below.
  • each adsorbent in order to immobilize the bacteria in slime based bakteo la capsule tooth (Rhodobacter capsulatus ), Rhodoseudomonas palustris , Bacillus thuringiensis and Bacillus subtilis and soaked for 24 hours.
  • the quantitative adsorbent is put into the adsorption pad (mesh size 700 ⁇ 700 ⁇ m, pad thickness 5mm, 5-stage connection) and into the culture vessel After immersion and suspension (the counterweight weight was determined according to Equation 1), the lid was closed and stored for 72 hours in an environment of 60% humidity and 20 ° C. Then, the adsorption pad was taken out of the culture vessel and the adsorption material to which bacteria were adsorbed was recovered through the opening and closing clip.
  • the average immersion depth (d) is a load weight (W L) 10kg (5kg counterweight 2), (5 2kg suction pad) Dead load (W S), 10kg of the suction pad when the bacteria are adsorbed adsorbent prepared, the suction pad
  • W L load weight
  • W S Dead load
  • Rhodobacter capsulatus Rhodoseudomonas palustris , Bacillus thuringiensis and Bacillus subtilis adsorbed as the adsorbents prepared by the above bacteria.
  • Scanning electron microscope (SEM) analysis results are shown in FIGS. 3 (using expanded calcite) and 4 (using high absorbent resin, observing internal and surface structure shapes) to evaluate adsorption performance. 3 shows the results for the case of not using the microorganism for comparison. As shown in FIG. 3 and FIG. 4, it can be seen that a good state of adsorption of bacteria (see the original part) is shown.
  • Rhodobacter is also known as Rhodobacter capsulatus ) was observed by scanning electron microscopy (SEM) of 1,000 ⁇ 10,000 magnification in order to evaluate the bacterial adsorption, the results are shown in Figure 14 and 15.
  • SEM scanning electron microscopy
  • Rhodobacter capsulatus was adsorbed, and Rhodobacter capsulatus was observed as a cluster in the highly porous resin. It also showed a similar shape in expanded vermiculite.
  • Rhodobacter capsulatus among the slime-forming bacteria was cultured for 7 days using maltose and dextrose selected as optimal medium conditions through slime production and growth evaluation.
  • Cultured Rhodobacter Rhodobacter capsulatus was immersed for 24 hours by using a highly porous resin having excellent surface structure and specific surface area.
  • Rhodobacter capsulatus was prepared by using a high porosity resin and an ocher-based binder adsorbed to form a slime.
  • the ocher-based binder is a binder used to prepare the slime bacteria-based coating material, using the ocher-based binder of the company.
  • the chemical composition ratio (X-ray fluorescence spectrometer; XRF) and X-ray diffraction analysis (XRD) of the loess-based binders used are shown in Table 6 and FIG. 16, respectively.
  • the loess-based binder used was calcined at 850 ° C.
  • the main components consisted of SiO 2 and C3A (Al 2 O 3 ) as measured by chemical composition ratio and X-ray diffraction.
  • the amorphous peak which is an amorphous crystalline phase was shown. Specific gravity and powder degree are 2.8 and 3,200 cm 2 / g, respectively.
  • Bacterial adsorbed high-porous resin and ocher-based binder (ocher cement) were weighed and mixed in a mixing vessel for at least 3 minutes to prepare a coating material, and the following experiments were carried out by coating with concrete using a brush. .
  • the thickness of the concrete coated with the coating material was measured using a vernier caliper after drying for 12 hours, the error range was ⁇ 0.5mm.
  • the coating material mixing experiment was classified into three groups and a total of 18 combination experiments were performed.
  • the mixing details in each group for mixing the coating material of the concrete are shown in Table 7 below.
  • Variables in each group are binder replacement rate, bacterial incorporation of adsorbent, and thickness of coating.
  • the substitution rate of the binder was the main variable, and the range of the substitution rate was set at 1 to 2 at 100 times the weight of bacteria incorporation into the adsorbent.
  • the second group was fixed at the binder replacement rate determined through the experimental results of the first group, the main variable is the amount of bacterial incorporation into the adsorbent, the range is 50 to 200 times the weight.
  • the binder replacement rate and the bacterial incorporation rate of the adsorbent were fixed according to the test results of the first group and the second group. In all groups, concrete and bacteria-free coatings were compared and analyzed. Comparative analysis results with the existing technology will be described later.
  • the mixing details of the concrete for applying the coating material in this Experimental Example are shown in Table 8 below.
  • the water-cement ratio was set to 0.45 in consideration of the design strength of the site-cast concrete for sewage facilities.
  • the cement used was S type 1 ordinary portland cement. Fine aggregates and coarse aggregates were made of natural sand with a maximum diameter of 5 mm or less, and crushed gravel with a maximum diameter of 25 mm, respectively.
  • the sand and crushed gravel used are 2.62 and 2.6, respectively, and the assembly rates are 2.5 and 6.3, respectively.
  • the moisture state of the aggregates used in the mixing was to maintain the surface dry saturation state.
  • a 300 liter capacity mixer was used to mix the concrete. Briefly describing the mixing method, first, the coarse beam was added to a 300 liter mixing vessel and coarse beam was added for 1 minute, and the cement was added for 1 minute and 30 seconds. Finally, water was added and the mixture was mixed for about 2 minutes. All formulations were free of water and air entrainers. In order to evaluate the sulfuric acid resistance of concrete, it was placed in a ⁇ 100 ⁇ 200 circular specimen mold. The cast specimens were demolded after 1 day and cured until 28 days of age in a constant temperature and humidity environment. Curing temperature is 20 ⁇ 2 °C and humidity is 60 ⁇ 5%.
  • the method of evaluating the performance of the coating material and the coating material coated coating material is as follows.
  • X-ray diffraction analysis is an analytical device in which X-rays are scattered by the crystal layer on the surface of a sample with an angle to obtain a diffraction image. This was used to analyze the main diffraction peaks of the hydration products of each sample.
  • Scanning electron microscope (SEM) and elemental analyzer (EDS) were used to analyze the internal microstructure and chemical components of the sample.
  • An electron beam was emitted to the sample to check the internal microstructure at 5,000 to 30,000 magnification.
  • the type and content of the elements contained in the surface were investigated by using X-ray energy emitted during electron beam irradiation.
  • XRD X-ray diffraction analysis
  • SEM electron microscopy
  • EDS elemental analyzer
  • the mold immersed in a 5% sulfuric acid solution was taken out and used for 1, 3, 7 and 28 days.
  • the surface of the surface was dried with a towel or the like, dried in a drying furnace at a temperature of 105 ⁇ 5 ° C., and the mass W (g) was measured using a 1g scale.
  • Equation 2 it is expressed as the ratio of the weight of the number of immersion days to the mass before immersion in sulfuric acid solution.
  • Equation 2 W represents the weight (%), W t represents the mass (kg) of the specimen by immersion days, W 0 represents the specimen mass (kg) before immersion.
  • the dynamic modulus of elastic modulus was evaluated according to the first resonance frequency.
  • the measurement was used by taking out the mold immersed in the solution on 28 days of immersion. It was measured using a resonance frequency measuring instrument (ERUDITE) of 500 ⁇ 10,000Hz capacity as a measuring device.
  • ERUDITE resonance frequency measuring instrument
  • the measured part was measured as an average value three times in each of the central part and the surface part.
  • the dynamic modulus by the resonance frequency test was expressed by Equations 3 and 4 Calculated by
  • Equation 3 E p represents the dynamic modulus of elasticity (MPa)
  • W represents the mass of the specimen (kg)
  • F represents the primary resonance frequency (Hz) of the longitudinal vibration
  • L represents the length of the specimen ( mm)
  • A represents the cross-sectional area (mm 2) of the specimen.
  • X-ray diffraction analysis (XRD) patterns according to the presence of bacteria in the coating material and the type of medium are shown in FIGS. 18 and 19.
  • XRD X-ray diffraction analysis
  • X-ray diffraction analysis showed that the gypsum crystal phase was found at around 10 ° C. for the coating material without bacteria. Gypsum formed causes a softening reaction of the coating material, which reacts with tricalcium silicate (C3S), a hydration reaction product, to form ettringite. Therefore, the coating material that is not mixed with bacteria is likely to increase in volume due to the formed gypsum and ettringite, thereby causing expansion and cracking. On the other hand, in the coating material mixed with bacteria, a large amount of silica silicate minerals (SiO 2 , Quartz) were formed.
  • silica silicate minerals SiO 2 , Quartz
  • SiO 2 is a fine particle having a particle size of 0.1 ⁇ 1 ⁇ m high reactivity and has the effect of filling the pores of cement particles. For this reason, as described below, the specimens containing bacteria showed higher compressive strength, which is considered to be due to the improved internal density due to SiO 2 in the coating material (Shiand Day, 2001). Ghosh (2009) also reported that SiO 2 was precipitated in mortars containing Shewanella bacteria that formed proteins around cells, thereby enhancing their strength. However, no reports have been made on SiO 2 formed by Rhodobacter capsulatus at this time. Therefore, it is expected that new organic-inorgainc crystals will be formed by slime, proteins, amino acids, etc., which are formed by bacteria.
  • Rhodobacter cultured in maltose medium Rhodobacter capsulatus -incorporated coating material increased the number of CaCO 3 Intensity peaks compared to the bacteria-free coating material, but the dextrose medium did not show any effect. Therefore, the species specificity of the bacteria and the medium (enzyme) determine the characteristics of the mineral crystal and it is expected that the species specificity and the medium (enzyme) effect can be imparted to improve the durability of the coating material.
  • the main variable of the first group is the bacterial adsorbent-binding ratio, and the appearance state of the test specimens by immersion days according to sulfuric acid immersion is shown in FIGS. 22 and 23.
  • appearance change of the uncoated test specimen (C) as the number of immersion days increased, the paste disappeared and the aggregate was exposed.
  • the coating material was severely peeled off as the adsorbent-binding ratio decreased.
  • the test specimen having a ratio of 1.0 exhibited a similar appearance to Test Sample C at 28 days of immersion.
  • the coating material peeled off at the corners as the immersion days increased with the adsorbent-binding ratio of 1.0. The effect on sulfuric acid erosion was minimal.
  • FIG. 24 shows the result of performing image analysis by dividing the cross section of the ⁇ 100 ⁇ 200mm specimen into four sections and cutting the cross section at 28 days of immersion to observe the depth of sulfuric acid penetration of the test specimen according to the binder substitution rate.
  • the depth of penetration was defined as the distance from the surface of the specimen to the point where white discoloration occurred.
  • the test sample (C) which was not coated with a coating material decolorized about 3.39 mm on the surface.
  • the white discoloration phenomenon was larger as the binder substitution rate decreased.
  • all the specimens containing bacteria did not show white discoloration on the surface of the concrete regardless of the dropping of the coating material.
  • FIGS. 25 and 26 The results of analyzing the main diffraction peaks of the reaction product of the sample taken from the concrete surface (0-1 cm) by using X-ray diffraction analysis (XRD) are shown in FIGS. 25 and 26. Scanning electron microscope (SEM) and elements The tissue structure and chemical member analysis results of the samples collected using the analyzer (EDS) are shown in FIGS. 27 to 29. All test samples in the main reaction product of plaster (Gypsum; CaSO 4 ⁇ H 2 O), silica (Quartz; SiO 2) and sulfur dioxide; a peak representing (Sulfur Oxide SO 2) was produced.
  • Figure 30 shows the weight change by number of days of sulfuric acid immersion of the first group.
  • the test specimen (C) without the coating material exhibited a weight loss of about 6-11% between 7 and 28 days of immersion.
  • the specimens containing no bacteria (G1-B1.0, G1-B1.5 and G1-B2.0) tended to decrease with decreasing adsorbent-binding ratio at 3 days of soaking, and at 28 days of soaking. In test specimens with an adsorbent-binding ratio of 1.5, the largest decrease was about 4%.
  • test samples containing bacteria cultured in maltose showed no significant effect on adsorbent-binding ratio on weight change at 3 days of immersion.
  • FIG. 31 shows the compressive strength reduction ratio (f ck / f ck (0) ) for each immersion day according to sulfuric acid immersion of the first group.
  • f ck is the compressive strength of the specimen according to the number of immersion days
  • f ck (0) is the compressive strength of the specimen before sulfuric acid immersion.
  • the compressive strength reduction ratio of the test specimen without the coating material was about 18% at 28 days of immersion, indicating a sharp drop in strength.
  • the compressive strength reduction ratios of the test specimens containing no bacteria were similar regardless of the adsorbent-binding ratio and immersion days.
  • the compressive strength reduction ratio of the test specimens incorporating bacteria cultured in dextrose medium increased or showed a similar tendency as the immersion days increased regardless of the adsorbent-binding ratio.
  • the compressive strength reduction ratio of the test specimens mixed with bacteria cultured in maltose medium was about 22-35% higher than the test sample without coatings, regardless of the number of immersion days and the adsorbent-binding ratio. It was about 3 ⁇ 10% higher than the test body. This is because the internal density of the coating material is improved due to the slime (glycocalxy) and silica component (SiO 2 ) formed by bacteria.
  • E d_c is the central elastic modulus at 28 days of immersion
  • E d_s is the dynamic modulus at the surface
  • E d_c (0) is the central elastic modulus at C specimens not eroded by sulfuric acid
  • E d_s (0) is It is the coefficient of dynamic elasticity of the surface part in test specimen C which is not eroded by sulfuric acid.
  • the reduction ratio was about 27 ⁇ 31% lower regardless of adsorbent-binding ratio.
  • the ratio of decrease in dynamic modulus of the central part of the test specimen containing bacteria was higher as the adsorbent-binding ratio increased, regardless of the type of medium. High.
  • the main variable of the second group is the amount of bacterial incorporation, and the appearance of concrete according to sulfuric acid immersion is shown in FIGS. 34 and 35.
  • Test specimens containing no bacteria (G2-W50, G2-W100 and G2-W200) were exposed to concrete due to peeling of the coating material on the surface at 28 days of immersion regardless of the amount of distilled water mixed.
  • slime bacteria-based test specimens showed no significant effect until 7 days of immersion except 50 times the amount of bacteria mixed with adsorbent, and at 28 days of immersion, there was little coating material on the surface regardless of the media type and the amount of bacteria mixed with adsorbent. Peeling occurred.
  • test specimens containing bacteria tended to increase the number and intensity of SiO 2 peaks as the amount of bacterial incorporation increased compared to the adsorbent, and a large amount of slime film formation and internal density in the internal structure were observed. The improvement was confirmed.
  • the specimens containing G2-M200 and G2-D200 containing bacteria 200 times the weight of the adsorbent showed a tendency of high Si element due to SiO 2 , and Mg, Na due to the medium and bacteria. , Cl and P and the like were further confirmed.
  • Figure 41 shows the weight change by number of immersion days according to the amount of bacteria mixed with the adsorbent.
  • the weight change of test specimens (G2-W50, G2-W100 and G2-W200) that did not contain bacteria decreased as the amount of distilled water mixed with the adsorbent increased with the number of immersion days.
  • the weight change at 3 days of immersion of the bacteria-incorporated test specimens had little effect on the presence of medium and the amount of bacterial incorporation.
  • the test samples containing G2-M50 and G2-D50 containing 50 times the bacteria to the weight of the adsorbent showed the greatest weight reduction compared to the other samples containing the bacteria.
  • the compressive strength reduction ratio of the test specimens (G2-W50, G2-W100, and G2-W200) that did not contain bacteria increased by 9 ⁇ 11% at 7 days of immersion regardless of the distilled water incorporation rate compared to the adsorbent, and 28 days of immersion. Esau increased about 12-15%.
  • the compressive strength reduction ratio of the test specimens incorporating the bacteria at 3 days of immersion had little effect on the amount of bacterial incorporation and the presence or absence of the medium. However, the compressive strength reduction ratio of the test sample containing bacteria was about 30% higher than the test sample without coating material at 28 days of immersion.
  • the ratio of lowering the compressive strength of the test body having 100% of bacteria incorporation to the adsorbent was about 4% higher than that of the test body having 100 times the amount of distilled water in the adsorbent. This is because the internal density of the coating material is improved due to the slime (glycocalxy) and silica component (SiO 2 ) formed by bacteria.
  • the ratios of decreasing the elastic modulus of elasticity E d_c / E d_c (0) ) and the surface modulus of elasticity ( E d_s / E d_s (0) ).
  • the central elastic modulus reduction ratio was similar in the range of 0.97 ⁇ 1.08 in all formulations.
  • the ratio of lowering the elastic modulus of the surface portion decreased as the amount of distilled water mixed with the adsorbent increased in the test specimens containing no bacteria (G2-W50, G2-W100 and G2-W200). This is because the strength of the coating material is lowered due to the increase in the water-binder ratio.
  • the ratio of decrease in dynamic modulus of the central part of the test specimen containing bacteria increased with increasing amount of bacteria compared to the adsorbent regardless of the type of medium.
  • the ratio of decrease in dynamic modulus of the surface portion of the specimen incorporating the bacteria was about 1.00 to 1.05, which was similar to that before the immersion.
  • EDS analysis also showed that the diffraction peaks were relatively low due to the consumption of calcium to form gypsum and ettringite.
  • test specimens containing bacteria having a coating thickness of 3.0 mm showed a tendency to increase the intensity of SiO 2 regardless of the type of medium. As a result, it is considered that the compressive strength and the sulfuric acid resistance improvement are higher than those of the other specimens.
  • Mg, Na, Cl and P was further confirmed due to the medium and bacteria.
  • test samples containing bacteria by immersion days showed about 1 ⁇ 2% higher or similar tendency than test samples containing no bacteria regardless of the type of medium and coating thickness.
  • FIG. 53 shows the compressive strength reduction ratio (f ck / f ck (0) ) for each immersion day according to sulfuric acid immersion of the third group.
  • the compressive strength reduction ratios of the test specimens containing no bacteria (G3-0.5, G3-1.0 and G3-3.0) were increased to about 8-10% with increasing immersion days except for 0.5 mm of coating thickness.
  • the compressive strength reduction ratio of the test specimens incorporating bacteria cultured in maltose medium tended to increase with increasing coating thickness at 3 days of immersion, and similar trends were observed in dextrose medium.
  • the compressive strength reduction ratio at 28 days of immersion was the largest in the test specimen having a coating thickness of 1.0 mm or more, regardless of the media type.
  • the ratio of lowering the compressive strength of the test specimen of G3-M3.0 containing bacteria was about 36% higher than that of the test specimen without coating material at 28 days of immersion, and the test specimen of G3-3.0 containing bacteria was not found. 4 ⁇ 6% higher than that. It is believed that the penetration of sulfuric acid is suppressed due to the improvement of the slime film and the internal density formed in the coating material, thereby improving the compressive strength.
  • the ratio of decrease in dynamic modulus of the surface portion of the test specimen having a coating thickness of 3 mm was about 21% higher than that of the test sample containing bacteria grown in maltose. In addition, it was about 8% higher than the test specimen of G3-D3.0 containing bacteria cultured in dextrose. This is thought to be due to the reaction product according to the amount of slime produced by bacteria and the erosion of sulfuric acid.
  • the slime bacteria-based coating material was selected as shown in Table 10 through the above experiment, and further blending was performed under the same conditions in order to evaluate the performance of the coating material according to the presence of bacteria. All specimens were applied identically with a brush. In addition, in order to make the coating thickness the same, the epoxy coating material was applied once, dried for 12 hours, and then applied twice. The coating thickness was fixed at 1.0 mm ⁇ 0.05 mm. In order to evaluate the sulfuric acid resistance of concrete, the change in appearance, weight, compressive strength and dynamic modulus of the concrete immersed in 5% sulfuric acid solution were evaluated according to JIS K 8951.
  • 57 shows the weight change of sulfuric acid immersion days.
  • the control group without the coating showed a weight loss of about 6-11% between 7 and 28 days of immersion.
  • epoxy coated concrete (Epoxy) showed a sharp weight loss of about 5% in 28 days of immersion.
  • the weights of all test specimens except for the control without the coating material (Control) showed a similar tendency with a decrease of about 5-6% at 28 days of immersion.
  • the compressive strength reduction ratio of the test specimen without the coating material exhibited a strength reduction of about 22% at 28 days of immersion.
  • the compressive strength reduction ratio of the test specimen coated with the epoxy coating material decreased as the number of immersion days increased.
  • the compressive strength reduction ratio of the test specimens containing bacteria increased with increasing immersion days, and the compressive strength reduction ratio of 28 days of immersion was higher than that of the test specimens without bacteria (Hawngtoh).
  • 59 and 60 are respectively sulfate immersion days 28 days copper modulus lowering of the center of the test piece in a non-(E d_c / E d_c (0 )) and the surface of the copper modulus lowering ratio (E d_s / E d_s (0 )) Indicated.
  • the central elastic modulus reduction ratio was similar in the range of about 0.97 ⁇ 1.06 in all specimens except the control without coating.
  • the ratio of decrease in dynamic modulus of the surface portion was about 6% higher than that of the test specimen coated with the epoxy coating (Hawngtoh), but about 9% lower than the test sample containing the bacteria.
  • Rhodoseudomonas palustris Rhodoseudomonas palustris
  • Bacillus thuringiensis as binders for the production of bacterial incorporation coatings
  • ⁇ -hemihydrate gypsum with a specific gravity of 2.67 g / cm 3 and blast furnace slag with a specific gravity of 2.91 g / cm 3 GGBS is used in a weight ratio of adsorbent-impregnated culture medium and 2.2: 1 (adsorbent-binding material ratio 2.2) and a binder mixed with a 1: 1 weight ratio of GGBS).
  • the experiment was conducted in the same manner as in Experimental Example 1, except that the results were as follows.
  • 61 shows the external appearance of the test specimens according to the sulfuric acid immersion days. All specimens did not show any apparent change in appearance at days 1 and 3 of soaking age. After 7 days of immersion, the test specimens using the coating material mixed with the concrete specimen (OPC) and Bacillus thuringiensis showed erosion in appearance, especially in the case of the concrete specimen (OPC). Appearance erosion was indicated. Rhodoseudomonas Test specimens using the coating material incorporating palustris ) did not show any noticeable appearance change.
  • 64 shows the results of the microstructure analysis for evaluating the adsorption of bacteria after 28 days of immersion in 5% sulfuric acid solution. Bacteria formation on the surface of the particles was confirmed in the coating material incorporating Rhodoseudomonas palustris and Bacillus thuringiensis .
  • Rhodobacter capsulatus Rhodoseudomonas palustris , Bacillus thuringiensis and Bacillus subtilis as binders for the production of bacterial incorporation coating materials as common binders with specific gravity of 3.15 g / cm3
  • Binder mixture of portalnd cement (OPC) and blast furnace slag (GGBS) with specific gravity of 2.91 g / cm3 in a 1: 1 weight ratio was mixed with a medium in which the adsorbent was immersed and a weight ratio of 2.2: 1 (adsorbent-binder ratio 2.2).
  • OPC portalnd cement
  • GGBS blast furnace slag
  • the experiment was conducted in the same manner as in Experimental Example 1, except that the adsorbent was used in a bacterial culture medium: expansive vermiculite weight ratio of 10: 1.
  • the experimental results are as follows.
  • the mass change at 7 days of immersion in sulfuric acid solution decreased by about 4% when only water and bacteria medium were used as the mixing water, and about 3 ⁇ 8% when using the bacterial inoculum culture solution as the mixing water. It was.
  • test specimen 66 shows the change in compressive strength of the test specimen depending on the immersion age. Test specimens using only water and bacterial media as the blended water decreased the compressive strength as the immersion age increased, whereas the compressive strength did not appear to decrease as the immersion age increased.
  • Rhodobacter capsulatus Rhodoseudomonas palustris , Bacillus thuringiensis and Bacillus subtilis as binders for the production of bacterial incorporation coating materials as ⁇ -half gypsum (2.67 g / cm 3)
  • a binder mixed with ⁇ -hemihydrate gypsum) and a blast furnace slag (GGBS) having a specific gravity of 2.91 g / cm3 in a 1: 1 weight ratio was mixed with a medium in which the adsorbent was immersed and a weight ratio of 2.2: 1 (adsorbent-binder ratio 2.2).
  • a coating material was prepared in the same manner as in Experimental Example 1 except that the adsorbent was used as the bacterial culture medium: expansive vermiculite in a weight ratio of 10: 1, and to evaluate the sustained growth of bacteria in a sulfuric acid corrosion environment. It was immersed in a 5% solution of sulfuric acid according to JSTM C 7401. Samples of the surface of the coating material were taken 7 days after the immersion age, and this was reinoculated into the medium (passage) to confirm the formation of colonies, and the results are shown in FIG. 67.
  • magnesia-phosphate complex shows a neutral pH of pH 7-9, and the initial reaction rate is very fast, and condensation starts within 5 to 15 minutes of pouring, thereby achieving high initial strength expression rate.
  • the adhesive strength of the magnesia-phosphate composite is higher than that of general cement, and when cement concrete is used as a base material, the adhesive strength is destroyed at the base material, not at the adhesive interface.
  • the loss of adhesion strength according to the moisture state of the water surface is small (generally, the water surface is wet), and the effect of strength on the curing temperature is less.
  • magnesia-phosphate complex has no concern about deterioration due to the maintenance time since the strength expression is not significantly affected by the curing temperature. Therefore, in the present invention, it is assumed that the magnesia-phosphate composite will play a sufficient role as a binder of the coating material according to the present invention.
  • Rhodobacter capsulatus magnesia-phosphate complex of various compositions (see Table 11 below) as a binder for the production of bacterial incorporation coating material in a weight ratio of 2: 1 (adsorbent-binding agent ratio 2) and the culture medium in which the adsorbent was immersed (adsorbent is A coating material was prepared in the same manner as in Experimental Example 1, except that the expanded vermiculite was used in a bacterial culture medium: expansive vermiculite weight ratio of 10: 1). The result of measuring pH is shown in FIG.
  • the phosphate content of the magnesia-phosphate composite is preferably 20 to 40% by weight, more preferably 30 to 40% by weight in order to achieve high compressive strength and pH retention performance of less than 10.
  • a retardant (Borax) is preferably added in an amount of 1 to 10 parts by weight, based on 100 parts by weight of the magnesia-phosphate complex, to a level of 3 to 5% by weight. It can be confirmed that it is more preferable to be added.
  • opening and closing clip 120 adsorption pad
  • opening and closing lid 160 screw opening
  • annular pin 210 concrete sphere

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Materials Engineering (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Ceramic Engineering (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Structural Engineering (AREA)
  • Hydrology & Water Resources (AREA)
  • Public Health (AREA)
  • Water Supply & Treatment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne, en tant que matériau de revêtement à base de biopellicule de bactéries utilisant des bactéries capables de former une biopellicule, un matériau de revêtement comprenant un adsorbant sur lequel des bactéries formant une biopellicule sont adsorbées et un liant. La présente invention concerne une bactérie optimale de formation de biopellicule et des conditions optimales de formation de biopellicule qui prennent en considération une résistance chimique du béton; un procédé d'adsorption optimal pour un environnement autotrophe viable pour des bactéries, plutôt qu'une injection simple, pendant le mélange d'un béton existant; une technique d'utilisation d'un liant optimal présentant un pH d'environ 8-10 tenant compte de l'environnement de croissance des bactéries; et un procédé qui est économique par rapport à une technique antérieure d'injection de bactéries et qui permet une adsorption facile et substantielle d'une grande quantité de bactéries. L'invention concerne également un nouveau matériau de revêtement qui améliore la résistance chimique du béton et la durabilité et qui prend en considération les mécanismes impliqués dans l'érosion chimique d'un égout en béton et les mécanismes impliqués dans la résistance à l'acide sulfurique de bactéries formant une biopellicule.
PCT/KR2016/014707 2016-12-15 2016-12-15 Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries Ceased WO2018110739A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/KR2016/014707 WO2018110739A1 (fr) 2016-12-15 2016-12-15 Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR2016/014707 WO2018110739A1 (fr) 2016-12-15 2016-12-15 Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries

Publications (1)

Publication Number Publication Date
WO2018110739A1 true WO2018110739A1 (fr) 2018-06-21

Family

ID=62558808

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2016/014707 Ceased WO2018110739A1 (fr) 2016-12-15 2016-12-15 Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries

Country Status (1)

Country Link
WO (1) WO2018110739A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109589911A (zh) * 2018-11-23 2019-04-09 江苏科技大学 一种改性高炉渣及其应用
CN113185230A (zh) * 2021-05-12 2021-07-30 郑州大学 一种适用于结构受拉区的微生物定位自修复混凝土材料及其制备方法
CN114105549A (zh) * 2021-12-31 2022-03-01 太仓市锦澄混凝土有限公司 一种具有高抗震性能的混凝土及其制备方法
CN116693267A (zh) * 2023-06-07 2023-09-05 珠海横琴超元科技有限公司 基于绿色建筑用储能天花墙体复合材料及其制造方法
KR20240088159A (ko) * 2022-12-13 2024-06-20 한국과학기술원 건축 구조물용 남조류 혼입 코팅재 및 그 제조 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002020693A (ja) * 2000-07-07 2002-01-23 Nippon Paint Co Ltd 微生物含有塗料、微生物含有塗膜及び付着防止方法
KR100374748B1 (ko) * 2000-06-03 2003-03-06 동부엔지니어링 주식회사 동물플랑크톤 육성방법과 이를 이용한 수질정화방법
KR20150134592A (ko) * 2014-05-22 2015-12-02 권은자 무기질 상온경화형 발포세라믹 조성물 및 그 제조방법

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100374748B1 (ko) * 2000-06-03 2003-03-06 동부엔지니어링 주식회사 동물플랑크톤 육성방법과 이를 이용한 수질정화방법
JP2002020693A (ja) * 2000-07-07 2002-01-23 Nippon Paint Co Ltd 微生物含有塗料、微生物含有塗膜及び付着防止方法
KR20150134592A (ko) * 2014-05-22 2015-12-02 권은자 무기질 상온경화형 발포세라믹 조성물 및 그 제조방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JEONG, JAE-EUN ET AL.: "Adsorption Technology for Bacteria-based Concrete Coating", JOURNAL OF TH E KOREAN RECYCLED CONSTRUCTION RESOURCES INSTITUTE, vol. 3, no. 2, June 2015 (2015-06-01), pages 140 - 145, XP055493376, DOI: doi:10.14190/JRCR.2015.3.2.140 *
JUNG, JAE EUN: "Effect of Bacteria Slime-based Coating on the Sulfate Resistance Behavior of Concrete", MASTER'S THESIS OF GRADUATE SCHOOL, 2015 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109589911A (zh) * 2018-11-23 2019-04-09 江苏科技大学 一种改性高炉渣及其应用
CN113185230A (zh) * 2021-05-12 2021-07-30 郑州大学 一种适用于结构受拉区的微生物定位自修复混凝土材料及其制备方法
CN113185230B (zh) * 2021-05-12 2022-08-16 郑州大学 一种适用于结构受拉区的微生物定位自修复混凝土材料及其制备方法
CN114105549A (zh) * 2021-12-31 2022-03-01 太仓市锦澄混凝土有限公司 一种具有高抗震性能的混凝土及其制备方法
KR20240088159A (ko) * 2022-12-13 2024-06-20 한국과학기술원 건축 구조물용 남조류 혼입 코팅재 및 그 제조 방법
KR102781413B1 (ko) 2022-12-13 2025-03-17 한국과학기술원 건축 구조물용 남조류 혼입 코팅재 및 그 제조 방법
CN116693267A (zh) * 2023-06-07 2023-09-05 珠海横琴超元科技有限公司 基于绿色建筑用储能天花墙体复合材料及其制造方法
CN116693267B (zh) * 2023-06-07 2024-01-16 珠海横琴超元科技有限公司 基于绿色建筑用储能天花墙体复合材料及其制造方法

Similar Documents

Publication Publication Date Title
WO2018110739A1 (fr) Développement de matériau de revêtement protecteur de béton à base d'une biopellicule de bactéries
Jeon et al. Investigation of sulfuric acid attack upon cement mortars containing silicon carbide powder
García-Lodeiro et al. Alkali–aggregate reaction in activated fly ash systems
Bellmann et al. Influence of sulfate solution concentration on the formation of gypsum in sulfate resistance test specimen
García de Lomas et al. Pozzolanic reaction of a spent fluid catalytic cracking catalyst in FCC-cement mortars
Irassar et al. Sulphate resistance of type V cements with limestone filler and natural pozzolana
Elmrabet et al. Study of properties of fly ash cements
Afshinnia et al. The potential of ground clay brick to mitigate Alkali–Silica Reaction in mortar prepared with highly reactive aggregate
Jeyaprabha et al. Effects of elevated temperature and water quenching on strength and microstructure of mortars with river sand substitutes
ES2921048T3 (es) Composición de hormigón y su método de producción
JP6361053B2 (ja) コンクリート保護コーティング材
Sun et al. Effects of belite incorporation on mechanical and microstructural properties of OPC mortars cured under carbonation condition
Buil et al. Creep of a silica fume concrete
Baghdadi Utilization of kiln dust in clay stabilization
JP4705846B2 (ja) セメントクリンカーおよびセメント組成物
KR100364911B1 (ko) 바텀에쉬를 이용한 콘크리트 제품 및 그 콘크리트제품의제조방법
JP2009185220A (ja) 地盤改良用固化材
Demirbaş Optimizing the physical and technological properties of cement additives in concrete mixtures
JP4860106B2 (ja) セメント混和材、セメント組成物、及びセメントコンクリート
KR101096012B1 (ko) 고화결합재 조성물 및 재활용재료를 이용한 수질정화기능을 갖는 친환경 콘크리트
JP3953469B2 (ja) 耐酸性コンクリート
Bhattacharjee Effect of relative proportion of pozzolana on compressive strength of concrete under different curing conditions
Nanthini et al. Experimental investigation of the durability of ambient-cured metakaolinbased geopolymer concrete in different sustainable environmental conditions
Cheah et al. Influence of different types and contents of gypsum on mechanical properties and porosity of sulphate activated binder
Samchenko et al. Improving crack resistance of concrete when using expanding cements

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 16923975

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 16923975

Country of ref document: EP

Kind code of ref document: A1