WO2018190577A1 - Microdisk, microdisk laser and sensor using same - Google Patents

Abstract

A microdisk is provided as an embodiment of the present invention. The microdisk has a whispering gallery mode which has a high-quality factor and has directionality to oscillate, the microdisk comprising: a first coating layer formed by coating a side surface of the microdisk with a dielectric material; and a second coating layer which is formed on the first coating layer and includes at least one monomolecular film or one polymer film, wherein the dielectric material may be an oxide or a nitride. Also, as one embodiment of the present invention, a biosensor using a microdisk with a high-quality factor may be provided.

Description

Micro Disc, Micro Disc Laser and Sensor Using the Same

The present invention relates to a micro disk, a micro disk laser, and a sensor using the same. More particularly, the present invention relates to a chemical sensor capable of accurately detecting chemical substances using a micro disk laser having a high quality value and oscillating in a single direction. . In addition, the present invention relates to a biosensor using an ultra-high-quality micro-resonator laser oscillating in a single direction as an example of a micro disk, a micro disk laser, and a sensor using the same. The present invention relates to a biosensor that is easy to bond, and does not cause deterioration of quality value when optically coupled to an optical waveguide, and has a high quality value, so that even when a single biomolecule is attached, a wavelength change occurs to measure an extremely small amount of biomaterial.

In recent years, researches using ring resonators have been actively conducted to develop chemical sensors capable of detecting even trace amounts of chemicals. However, since the ring resonator does not have a high quality value, the chemical sensor using the ring resonator has a limitation in that it cannot accurately detect chemicals.

In order to develop a more sensitive chemical sensor, research is being conducted to apply a troid type resonator or a spherical resonator having a higher quality than a ring resonator to a chemical sensor. However, the toroidal resonator or the spherical resonator has to combine the optical waveguide with the resonator because the light does not go out in one direction, but such coupling has a problem of reducing the quality value. In addition, in order to couple the laser or the resonator with the optical fiber is required to fine control the distance is not easy to control, when using the optical fiber is unstable optical coupling in the weak vibrations of the external it is difficult to apply as a chemical sensor.

Apart from the development of chemical sensors using troid type resonators or spherical resonators, chemical sensors using chaotic resonators have also been reported. However, in the chaotic resonator, the trajectory of the laser resonator internal mode is not bound to the edge of the resonator but is bound to an unstable periodic track or a stable periodic track. In this case, a problem may occur that the chemical sensor does not detect the chemical. In order to solve the above problem, the oscillation resonance mode of the laser must be bound to the edge of the laser. A resonator with this characteristic must be able to form a whispering gallery mode, and the whispering gallery mode should oscillate in a single direction.

Korean Patent Registration No. 10-0640071 (hereinafter referred to as Patent Document 1) proposes a micro disk laser having a unidirectional oscillation characteristic. Patent document 1 discloses that light formed in whispering gallery mode can be oscillated in a single direction. However, in order to make a chemical sensor using the micro disk laser disclosed in Patent Document 1, a chemical substance to be detected must be adsorbed onto the surface of the micro disk. However, there is a problem that chemical substances cannot be selectively adsorbed on the surface of a generally manufactured micro disk, and thus it is not easy to manufacture a chemical sensor using the micro disk.

Therefore, there is a need for a chemical sensor capable of precisely detecting a chemical by using a micro disk to selectively adsorb the chemical to be detected.

In addition, in recent years, research to develop a biosensor using a ring resonator has been actively conducted. This is because the high quality of the ring resonator enables the measurement of the wavelength variation due to the attachment of the biomaterial, thereby detecting the biomaterial. However, the problem of the ring resonator is that it has a limitation as a precision biosensor due to its high quality. Higher sensitivity than the cyclic resonator allows the development of biosensors with higher sensitivity. Toroidal or spherical resonators are extremely high in quality and are gaining attention as biosensors. However, the problem of such a circular resonator is to couple an optical waveguide, such as an optical fiber, close to the resonator in order to inject light into the resonator, thereby creating a resonance mode called a whispering gallery bound to the edge of the resonator. Toroidal resonators can also be used to make lasers. These circular resonator lasers emit light uniformly in all directions and combine a waveguide, such as an optical fiber, with the laser. The problem with this circular resonator is that the light cannot go out in one direction, so the waveguide must be combined with the laser, which causes the quality value to fall. In addition, fine distance adjustment is required to combine the laser or resonator with the optical fiber. This fine distance adjustment is not easy, and when the optical fiber is used, it is difficult to use as a biosensor because the coupling is broken even in the external weak vibration.

Apart from the development of such a biosensor using a circular resonator, a biosensor using a chaotic resonator has been reported. However, in the chaotic resonator, the trajectory of the mode inside the resonator of the laser is not bound to the edge of the resonator but is bound to an unstable periodic track or a stable periodic track. In this case, even if there is a biomaterial on the surface, there is a problem that the laser does not detect the biomaterial. In order to solve the above problem, the oscillation resonance mode of the laser must be bound to the edge of the laser. A resonator with this characteristic should be a resonator capable of creating a whispering gallery mode, which is a confined mode along the edge of the resonator. It is only a circular resonator known to date. Recently, chaotic resonators have been developed in which whispering gallery mode oscillates in a single direction. However, there is no biosensor using the resonator which oscillates in a single direction while the whispering gallery mode is bound to the edge of the resonator as described above. To date, the chaotic resonator with the highest grade value has a passive measurement value of less than 200,000, which limits the measurement. So far, no biosensor has been developed that uses a chaotic resonator with a whispering gallery-type resonance mode that oscillates in a single direction.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and in the manufacture of a chemical sensor using a micro-disc laser which has a high quality value and oscillates a laser beam of whispering gallery mode in a single direction, The present invention aims to provide a chemical sensor capable of precisely detecting a specific substance by using the characteristic of selective binding of a specific substance according to the treated compound on the surface of the micro disk.

In addition, an object of the present invention relates to a precision biosensor fabricated using a high quality micro disk laser oscillating in a single direction, and because of the high quality value, the line width of the laser is narrow, so even at low concentrations, a small wavelength variation or an antigen-antibody reaction occurs. This is to make it possible to easily measure small wavelength variations caused by reactions between nucleic acids and use them in diagnosing diseases. In order to make a biosensor using extremely high-quality lasers, the surface of the laser (ex. Side) is chemically treated with an antibody, or a nucleic acid is added to change the wavelength through the antigen-antibody reaction or the wavelength generated by the reaction between nucleic acids. By measuring the microvariation of the specific bio-materials by selectively measuring to diagnose the disease.

According to an embodiment of the present invention, the micro disk has a high quality whispering gallery mode, whispering gallery mode is a directional oscillation, the first coating layer formed by coating a dielectric on the side of the micro disk; And a second coating layer formed on the first coating layer, the second coating layer including one or more monomolecular or polymer films, wherein the dielectric is an oxide or nitride.

The micro disc may be formed of a semiconductor, a solid medium or a polymer to which a pigment is added.

Oxides include TiO ₂ , MgO, K ₂ O, Al ₂ O ₃ , Li ₂ O, Na ₂ O, Rb ₂ O, Cs ₂ O, BeO, CaO, SrO, BaO, B ₂ O ₃ , Ga ₂ O ₃ , In ₂ O ₃ , Ti ₂ O ₃ , SiO ₂ , GeO ₂ , SnO ₂ , PbO ₂ , P ₄ O ₁₀ , As ₂ O ₅ , Sb ₂ O ₅ , Bi ₂ O ₅ , SeO ₃ , TeO ₃ , PoO ₃ , I ₂ O ₇ and At ₂ O ₇ is an oxide selected from, the nitride is Li ₃ N, Na ₃ N, K ₃ N, Mg ₃ N ₂ , Be ₃ N ₂ , Ca ₃ N ₂ , Sr ₃ N ₂ , ScN, Fe ₂ N, Cu ₃ N, Zn ₃ N ₂ , (CN) ₂ . It may be a nitride selected from S ₄ N ₄ , Se ₄ N _{4 ,} GaN and SiN.

The monomolecular film and the polymer film may be attached onto the first coating layer.

The monomolecular film and the polymer film may be selectively combined with a specific material.

As the micro disc selectively binds to a monomolecular film or a polymer film, the micro disc can detect the specific material by measuring a change in wavelength generated in the resonance mode of the micro disc.

The micro disc laser including the micro disc includes an excitation portion capable of exciting the edge of the micro disc, and can oscillate in one direction a high-quality whispering gallery mode bound to the interface edge of the micro disc.

The excitation portion may excite the edge of the micro disk, ie, the region in which the whispering gallery mode is bound, by light or current. The whispering gallery mode may include a whispering gallery mode that is bound along the edge of the boundary of the micro disc or a whispering gallery mode that is bound along a periodic trajectory adjacent to the side of the micro disc.

The resonant mode in the form of whispering gallery mode bound along a periodic trajectory adjacent to the side of the micro disk may include a resonant mode in the form of a whispering gallery mode bound along a stable trajectory of period 4 or more or an unstable trajectory of period 4 or more. .

The chemical sensor includes a measuring unit for measuring the oscillation wavelength value of the micro-disc laser and the laser beam oscillated from the micro-disc laser, and detects a specific substance using a change in the oscillation wavelength value of the laser beam measured by the measuring unit. can do.

The change in the oscillation wavelength value of the laser beam measured by the measuring unit is generated as the specific material is bonded to the second coating layer of the micro disc. In addition, the measuring unit may include a spectrometer.

The change in wavelength value oscillating through the micro disk can be measured by optical beating with the wavelength oscillating in another micro disk or the wavelength oscillating in another high quality laser.

In addition, as an embodiment of the present invention, a biosensor using a high quality micro disk may be provided.

In a biosensor using a micro disk in which the whispering gallery mode has a high quality value, the micro disk has a single direction in which the whispering gallery mode has a stronger intensity of oscillation in one direction than an oscillation intensity in the other direction. Oscillated with a semiconductor, manufactured using a semiconductor, a solid medium or a polymer, the micro disc may be coated with a dielectric material on its side to enable chemical bonding with a virus, a bacterial antibody or a nucleic acid.

The dielectric material is an oxide or nitride (eg, GaN, SiN or SiO _2, etc.), and the antibody or nucleic acid can be attached to the micro disc through a chemical reaction on the side coated with the dielectric material.

In addition, a predetermined antigen can be bound to the antibody through the antigen-antibody reaction in the micro disk, and whether or not the predetermined antigen is bound by the change of the wavelength of the resonance mode in the micro disk by the antigen bound to the antibody can be detected. Can be.

Predetermined nucleic acids can be bound through a reaction between nucleic acids in the micro disk, and whether or not the predetermined nucleic acids are bound by the change of the wavelength of the resonance mode in the micro disk by the bound nucleic acids.

The micro-disc of the biosensor according to an embodiment of the present invention has an excitation device to oscillate a whispering gallery-type mode having a directivity, and the biosensor may include a micro-disc laser using the micro-disc.

In addition, the micro disk laser may be a dye laser in which a dye is added to a semiconductor, a solid medium, or a polymer.

The micro-disc laser can be excited with light or current in an area where the incident angle of the boundary surface of the resonator is larger than the critical angle in the Birkhoff coordinate system where the propagation of light inside the resonator is confused.

In addition, the biosensor may include a plurality of micro disks, and a plurality of micro disks are attached to different antibodies, and a predetermined antibody is based on the degree of variation in wavelength during antigen-antibody reaction in each micro disk. Biomaterial can be detected.

The biosensor may include a plurality of micro disks, each of which has a different nucleic acid attached thereto, and a predetermined nucleic acid to be detected based on the degree of variation in wavelength during the reaction between nucleic acids in each micro disk. Biomaterials can be detected.

Predetermined biomaterials may be detected using a lookup table that represents the degree of variation in wavelength according to the reaction between different antibodies and the given biomaterial.

In addition, a lookup table showing a degree of variation in wavelength depending on the degree of attachment of a predetermined biomaterial, which is a detection target nucleic acid, to each different nucleic acid attached to each micro disk is used to detect a predetermined biomaterial. Can be.

The biosensor may include a plurality of micro disks, some of the micro disks have an antibody attached thereto, others have a nucleic acid attached thereto, and antigens to the antibody according to the change of wavelength in the micro disk by the antigen-antibody reaction. Is detected, whether or not the detection target nucleic acid is bound to the nucleic acid according to the change of the wavelength in the micro disk by the reaction between nucleic acids, whether the antigen binding and the binding of the nucleic acid can be detected at the same time. .

Further, the biomaterial to be detected based on predetermined data on the degree of variation in wavelength due to antigen-antibody reaction with the antibody attached to the micro disk or the degree of variation in wavelength due to internucleic acid reaction with the nucleic acid attached to the micro disk. This can be detected and the data can be in the form of a function or graph.

The micro-disc according to an embodiment of the present invention has a high-quality whispering gallery mode, the whispering gallery mode can be oscillated in a single direction, the dielectric is coated on the side of the micro-disc to form a first coating layer, By forming a second coating layer including a monomolecular film or a polymer film on the first coating layer, a specific material may be selectively bonded to the monomolecular film or the polymer film. High-quality whispering gallery mode can be used to fabricate a chemical sensor using a micro-disc laser that includes a micro-disc that emits light in a single direction, enabling the precise detection of certain substances even in low gas environments. In addition, by using the chemical sensor of an embodiment of the present invention by measuring a small change in the wavelength generated as the specific material is selectively bonded to the monomolecular film or polymer film included in the second coating layer of the micro disk, the type and The concentration can be measured precisely.

In addition, using the biosensor using a high-definition microdisk according to an embodiment of the present invention, not only can accurately diagnose diseases using antigen-antibody reactions, but also can detect diseases and the like by using nucleic acids. It can also be diagnosed. In other words, since the nucleic acid and the antibody can be attached to the micro-disc resonator laser inside the same microfluidic, the antigen-antibody reaction and the reaction between the nucleic acids can be simultaneously measured, so that diseases can be diagnosed faster and more accurately than before. Therefore, the efficiency of disease diagnosis can be improved.

1A and 1B are diagrams schematically illustrating a micro disk according to an embodiment of the present invention.

2 is a view schematically illustrating that a monomolecular film is formed on a first coating layer.

3 is a view schematically showing that a polymer film is formed on a first coating layer.

4 is a view schematically showing that a specific material is selectively bonded on the polymer film.

5 is a view schematically showing that a specific material is combined with a polymer.

FIG. 6 is a view showing a state in which a micro disk side is coated with SiO ₂ , GaN, or the like according to an embodiment of the present invention.

7 is a view showing a state in which the antibody is attached to the side after chemically treating the micro-disk side.

8 is a view showing a state in which an artificial nucleic acid is attached to the side after chemically treating the micro disk side.

9 is a diagram showing an antigen-antibody reaction in which antigen binds to an antibody attached to the side of a micro disk.

10 is a diagram illustrating a reaction in which a nucleic acid of a virus or a bacterium binds to an artificial nucleic acid attached to a side of a micro disk.

11 is a schematic diagram of passing a biomaterial through a microfluidic to a biosensor comprising a resonator laser composed of microdisks.

12 is a schematic diagram showing a method for diagnosing a disease or the like by simultaneously measuring an antigen-antibody reaction and a reaction between nucleic acids.

According to an embodiment of the present invention, the micro disk has a high quality whispering gallery mode, whispering gallery mode is a directional oscillation, the first coating layer formed by coating a dielectric on the side of the micro disk; And a second coating layer formed on the first coating layer, the second coating layer including one or more monomolecular or polymer films, wherein the dielectric is an oxide or nitride.

The micro disc may be formed of a semiconductor, a solid medium or a polymer to which a pigment is added.

Oxides include TiO ₂ , MgO, K ₂ O, Al ₂ O ₃ , Li ₂ O, Na ₂ O, Rb ₂ O, Cs ₂ O, BeO, CaO, SrO, BaO, B ₂ O ₃ , Ga ₂ O ₃ , In ₂ O ₃ , Ti ₂ O ₃ , SiO ₂ , GeO ₂ , SnO ₂ , PbO ₂ , P ₄ O ₁₀ , As ₂ O ₅ , Sb ₂ O ₅ , Bi ₂ O ₅ , SeO ₃ , TeO ₃ , PoO ₃ , I ₂ O ₇ and At ₂ O ₇ is an oxide selected from, the nitride is Li ₃ N, Na ₃ N, K ₃ N, Mg ₃ N ₂ , Be ₃ N ₂ , Ca ₃ N ₂ , Sr ₃ N ₂ , ScN, Fe ₂ N, Cu ₃ N, Zn ₃ N ₂ , (CN) ₂ . It may be a nitride selected from S ₄ N ₄ , Se ₄ N _{4 ,} GaN and SiN.

The monomolecular film and the polymer film may be attached onto the first coating layer.

The monomolecular film and the polymer film may be selectively combined with a specific material.

As the micro disc selectively binds to a monomolecular film or a polymer film, the micro disc can detect the specific material by measuring a change in wavelength generated in the resonance mode of the micro disc.

The micro disc laser including the micro disc includes an excitation portion capable of exciting the edge of the micro disc, and can oscillate in one direction a high-quality whispering gallery mode bound to the interface edge of the micro disc.

The excitation portion may excite the edge of the micro disk, ie, the region in which the whispering gallery mode is bound, by light or current. The whispering gallery mode may include a whispering gallery mode that is bound along the edge of the boundary of the micro disc or a whispering gallery mode that is bound along a periodic trajectory adjacent to the side of the micro disc.

The resonant mode in the form of whispering gallery mode bound along a periodic trajectory adjacent to the side of the micro disk may include a resonant mode in the form of a whispering gallery mode bound along a stable trajectory of period 4 or more or an unstable trajectory of period 4 or more. .

The chemical sensor includes a measuring unit for measuring the oscillation wavelength value of the micro-disc laser and the laser beam oscillated from the micro-disc laser, and detects a specific substance using a change in the oscillation wavelength value of the laser beam measured by the measuring unit. can do.

The change in the oscillation wavelength value of the laser beam measured by the measuring unit is generated as the specific material is bonded to the second coating layer of the micro disc. In addition, the measuring unit may include a spectrometer.

The change in wavelength value oscillating through the micro disk can be measured by optical beating with the wavelength oscillating in another micro disk or the wavelength oscillating in another high quality laser.

In addition, in a biosensor using a microdisc in which the whispering gallery mode has a high quality value, the microdisc has a single whispering gallery mode in which the intensity of oscillation in one direction is stronger than that of the oscillation in the other direction. Oscillated with aroma, and made using a semiconductor, a solid medium or a polymer, the micro disc may be coated with a dielectric material on its side to enable chemical bonding with a virus, bacterial antibody or nucleic acid.

The dielectric material is an oxide or nitride (eg, GaN, SiN or SiO _2, etc.), and the antibody or nucleic acid can be attached to the micro disc through a chemical reaction on the side coated with the dielectric material.

In addition, a predetermined antigen can be bound to the antibody through the antigen-antibody reaction in the micro disk, and whether or not the predetermined antigen is bound by the change of the wavelength of the resonance mode in the micro disk by the antigen bound to the antibody can be detected. Can be.

Predetermined nucleic acids can be bound through a reaction between nucleic acids in the micro disk, and whether or not the predetermined nucleic acids are bound by the change of the wavelength of the resonance mode in the micro disk by the bound nucleic acids.

The micro-disc of the biosensor according to an embodiment of the present invention has an excitation device to oscillate a whispering gallery-type mode having a directivity, and the biosensor may include a micro-disc laser using the micro-disc.

In addition, the micro disk laser may be a dye laser in which a dye is added to a semiconductor, a solid medium, or a polymer.

The micro-disc laser can be excited with light or current in an area where the incident angle of the boundary surface of the resonator is larger than the critical angle in the Birkhoff coordinate system where the propagation of light inside the resonator is confused.

In addition, the biosensor may include a plurality of micro disks, and a plurality of micro disks are attached to different antibodies, and a predetermined antibody is based on the degree of variation in wavelength during antigen-antibody reaction in each micro disk. Biomaterial can be detected.

The biosensor may include a plurality of micro disks, each of which has a different nucleic acid attached thereto, and a predetermined nucleic acid to be detected based on the degree of variation in wavelength during the reaction between nucleic acids in each micro disk. Biomaterials can be detected.

Predetermined biomaterials may be detected using a lookup table that represents the degree of variation in wavelength according to the reaction between different antibodies and the given biomaterial.

In addition, a lookup table showing a degree of variation in wavelength depending on the degree of attachment of a predetermined biomaterial, which is a detection target nucleic acid, to each different nucleic acid attached to each micro disk is used to detect a predetermined biomaterial. Can be.

The biosensor may include a plurality of micro disks, some of the micro disks have an antibody attached thereto, others have a nucleic acid attached thereto, and antigens to the antibody according to the change of wavelength in the micro disk by the antigen-antibody reaction. Is detected, whether or not the detection target nucleic acid is bound to the nucleic acid according to the change of the wavelength in the micro disk by the reaction between nucleic acids, whether the antigen binding and the binding of the nucleic acid can be detected at the same time. .

Further, the biomaterial to be detected based on predetermined data on the degree of variation in wavelength due to antigen-antibody reaction with the antibody attached to the micro disk or the degree of variation in wavelength due to internucleic acid reaction with the nucleic acid attached to the micro disk. This can be detected and the data can be in the form of a function or graph.

DETAILED DESCRIPTION Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings so that those skilled in the art may easily implement the present invention. As those skilled in the art would realize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the present invention. In the drawings, parts irrelevant to the description are omitted in order to clearly describe the present invention, and like reference numerals designate like parts throughout the specification.

Terms used herein will be briefly described and the present invention will be described in detail.

The terms used in the present invention have been selected as widely used general terms as possible in consideration of the functions in the present invention, but this may vary according to the intention or precedent of the person skilled in the art, the emergence of new technologies and the like. In addition, in certain cases, there is also a term arbitrarily selected by the applicant, in which case the meaning will be described in detail in the description of the invention. Therefore, the terms used in the present invention should be defined based on the meanings of the terms and the contents throughout the present invention, rather than the names of the simple terms.

When any part of the specification is to "include" any component, this means that it may further include other components, except to exclude other components unless otherwise stated. In addition, when a part of the specification is said to be "connected" or "coupled" to another part, it is not only "directly connected (or coupled)", but also "with another element in the middle". This includes the case where it is connected (or coupled).

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

The micro disk 100 according to an embodiment of the present invention has a high quality whispering gallery mode, the whispering gallery mode is oscillated with a direction, the side of the micro disk 100 is formed by coating a dielectric First coating layer 210; And a second coating layer 220 formed on the first coating layer 210 and including at least one monolayer 221 or a polymer layer 222, wherein the dielectric may be an oxide or a nitride.

In addition, the micro-disc 100 has a whispering gallery mode oscillating in a single direction, and a dielectric coating is coated on the side of the micro-disc 100 to form a first coating layer 210, and the monomolecular layer 221 or the polymer layer 222 may be formed. By including the second coating layer 220 is formed on the first coating layer 210, a specific material may be selectively bonded to a monomolecular film or a polymer film.

The whispering gallery mode bound to the edge of the micro disc 100 may have a high quality value. As light or current is supplied to one surface of the micro disc 100 to excite the micro disc 100, a whispering gallery mode that is bound to the edge of the micro disc 100 may be formed along the edge of the micro disc 100. Can be. The Q value, which is the quality value of the whispering gallery mode bound to the edge of the micro disc 100, is 10 ⁴ or more, and may have a high quality value.

In addition, the micro disk 100 may be, for example, a deformed circle, a shape consisting of four arcs, a shape consisting of three circles and one straight line, an ellipse shape such as an egg shape, a shape in which a heart shape and an arc are combined, and the like. It may have a shape that cannot be integrated. The non-integral shape means a shape in which the Helmholtz equation is separated in two dimensions so that an internal wave function cannot be obtained analytically. The micro-disc laser using the micro-disc 100 having the above-described shape may oscillate the laser beam in the whispering gallery mode bound to the edge of the micro-disc 100 in a single direction.

The micro-disc 100 can oscillate a laser beam having a high quality value in a single direction, so that the light coupling with the optical waveguide is easy and the damage of the quality value can be suppressed in the process of combining with the optical waveguide. When the disk 100 is applied to a chemical sensor, the detection precision of the chemical sensor can be improved.

The micro disc 100 may be formed of a polymer to which a semiconductor, a solid medium, or a pigment is added. For example, a III-V semiconductor material may be used as the semiconductor, and a GaAs series, InGaAsP, GaN series, or the like may be used as the III-V semiconductor material. In addition, as a solid medium, various solid laser media such as Nd: YAG, Nd: Glass, NdYVO ₄ , Sapphire doped with impurities, and ruby may be used.

As the polymer to which the dye is added, for example, a polymer to which a dye produced by adding a number of pigments such as Rhodamine 6G and Rhodamine B to a number of polymers such as polymethylmethacrylate (PMMA) can be used.

However, the kind of the polymer to which the above-described semiconductor, solid medium or pigment is added is merely an example for description, and is not intended to limit the kind of the polymer to which the semiconductor, solid medium or dye is added.

1A and 1B are diagrams schematically illustrating a micro disk according to an embodiment of the present invention.

1A, 1B, and 1C, a first coating layer 210 may be formed on a side of the micro disc 100, and a second coating layer 220 may be formed on the first coating layer 210.

The first coating layer 210 may be formed by coating a dielectric of an oxide or nitride on the surface of the micro disc 100. After etching the micro disc 100, an oxide or nitride may be coated on the surface of the micro disc 100 through passivation. By forming a first coating layer 210 by coating a dielectric on the side of the micro disk 100, it is possible to suppress the occurrence of leakage current when the current is supplied to the micro disk 100, and the second coating layer 220 may be micro It can be easily introduced into the disk 100.

These oxides are TiO ₂ , MgO, K ₂ O, Al ₂ O ₃ , Li ₂ O, Na ₂ O, Rb ₂ O, Cs ₂ O, BeO, CaO, SrO, BaO, B ₂ O ₃ , Ga ₂ O ₃ , In ₂ O ₃ , Ti ₂ O ₃ , SiO ₂ , GeO ₂ , SnO ₂ , PbO ₂ , P ₄ O ₁₀ , As ₂ O ₅ , Sb ₂ O ₅ , Bi ₂ O ₅ , SeO ₃ , TeO ₃ , PoO ₃ , I ₂ O ₇ and At ₂ O ₇ is an oxide selected from, the nitride is Li ₃ N, Na ₃ N, K ₃ N, Mg ₃ N ₂ , Be ₃ N ₂ , Ca ₃ N ₂ , Sr ₃ N ₂ , ScN, Fe ₂ N, Cu ₃ N, Zn ₃ N ₂ , (CN) ₂ . It may be a nitride selected from S ₄ N ₄ , Se ₄ N _{4 ,} GaN and SiN, and may be formed as a first coating layer on the surface of the micro disc 100.

The oxide or nitride used to form the first coating layer 210 on the surface of the micro disc 100 may be selected according to the types of the monomolecular film 221 and the polymer film 222 included in the second coating layer 220. For example, when the monolayer 221 is formed of a compound having a molecular structure of (R′O) ₃ —Si—R, SiO ₂ may be used as an oxide used in the first coating layer 210.

The second coating layer 220 is formed on the first coating layer 210 and may include one or more monolayers 221 or a polymer layer 222. In detail, the second coating layer 220 may be formed of a single monolayer 221 or a polymer layer 222, and may be formed of a plurality of monolayer 221 or a plurality of polymer layers 222. For example, the first monomolecular film may be formed on the first coating layer 210 using a single molecule, and new monomolecules different from the single molecule constituting the first monomolecular film may be formed by using a functional group present in the first monomolecular film. May be combined with the first monolayer to form a second monolayer. Similarly, the first polymer film and the second polymer film may be sequentially coated on the first coating layer 210.

Furthermore, after the monolayer 221 is formed on the first coating layer 210, the polymer layer 222 is formed on the monolayer 221, and the second coating layer 220 including the monolayer 221 and the polymer layer 222. ) May be formed on the first coating layer 210.

By forming the second coating layer 220 including at least one monolayer 221 or the polymer layer 222 on the first coating layer 210, it is possible to control the thickness of the coating layer coated on the surface of the micro disc 100, A monomolecular film 221 or a polymer film 222 that can selectively bind to a material may be introduced to the surface of the micro disc 100.

The monolayer 221 and the polymer layer 222 may be attached on the first coating layer 210. In detail, the monolayer 221 or the polymer layer 222 may be physically adsorbed or chemically bonded on the first coating layer 210 formed of an oxide or nitride to form the second coating layer 220.

For example, when SiO ₂ is coated on the surface of the micro disc 100 to form the first coating layer 210, the monomolecular film 221 using a hydroxyl group (-OH) present in the first coating layer 210. ) Or the polymer film 222 may be formed on the first coating layer 210. When the second coating layer 220 is formed on the first coating layer 210 by using a compound having a molecular structure of (R′O) ₃ -Si-R, the hydroxyl group present in the first coating layer 210 is formed. As the condensation reaction proceeds between the (R′O) ₃ —Si—R compounds and HOR ′ is released, a —O—Si—R bond is newly formed on the first coating layer 210, the monolayer 221 is formed on the first coating layer 210. It may be formed on the coating layer 210. In this case, R may be polyamiline, polythiophene, polypyrrole, polyphenenylene, or the like.

2 is a view schematically illustrating that a monomolecular film is formed on a first coating layer.

The monolayer 221 or the polymer layer 222 may be formed on the first coating layer 210. For example, in the -O-Si-R bond formed on the first coating layer 210, all of the monomolecular films 221 or the polymer film 222 that can be bonded to Si are R, and according to the monomolecule of the R or the functional group of the polymer, Selective adsorption occurs where certain chemicals are more readily adsorbed. In addition, after the monomolecular film is formed, a new monomolecular film may be formed on the existing monomolecular film by using a functional group present in the monomolecular film.

3 is a view schematically showing that a polymer film is formed on a first coating layer.

Through physical adsorption or chemical bonding, the polymer film 222 may be formed on the first coating layer 210. When the polymer film 222 is physically adsorbed onto the first coating layer 210, for example, a polymer solution in which the polymer is dissolved in a solvent is spin coated and dips on the surface of the first coating layer 210 of the micro disc 100. The coating or coating may be performed by spray coating to form the polymer film 222. As the polymer, for example, a styrene polymer, an acrylic polymer, a methacryl polymer, a vinyl polymer, a urethane polymer, or the like may be used, and the second coating layer 220 may be formed by selecting a polymer that can be combined with a specific material. have.

In addition, the polymer film 222 may be formed on the first coating layer 210 through chemical bonding. Referring to FIG. 3, the polymer film 222 may be formed on the first coating layer 210 by using a functional group present in the first coating layer 210. For example, SiO ₂ may be coated on the surface of the micro disc 100 to form the first coating layer 210, and the monomolecular layer 221 may be formed using the —OH group present in the first coating layer. The polymer film 222 may be formed using a functional group.

Therefore, since the monomolecular film 221 or the polymer film 222 included in the second coating layer 220 may be stably adsorbed or combined with the first coating layer 210, the monomolecular film 221 or the polymer film selectively bondable with a specific material. The 222 can be easily introduced into the micro disc 100.

4 is a view schematically showing that a specific material is selectively bonded on the polymer film.

The monolayer 221 and the polymer layer 222 may be selectively combined with a specific material. In addition, as the specific material is selectively coupled to the monomolecular film 221 or the polymer film 222, the specific material may be detected by measuring a change in the wavelength generated in the resonance mode of the micro disc 100.

The monomolecular film 221 and the polymer film 222 included in the second coating layer 220 may be selectively chemically bonded or chemically adsorbed with a specific material. Referring to FIG. 4, when a gas containing a specific material is supplied to the micro disc 100 coated with the first coating layer 210 and the second coating layer 220, the monomolecular film included in the second coating layer 220 ( Depending on the type of the 221 or the polymer film 222, the type of a specific material that may be chemically bonded or chemically adsorbed to the second coating layer 220 may be different.

5 is a view schematically showing that a specific material is combined with a polymer.

For example, as shown in FIG. 5, A linear hydrogen-bond acidic linear functionalized polymer may be selectively combined with DMMP gas, Sarin gas and 2-CEES gas. In other words, a specific material (eg, DMMP gas, etc.) may be selectively bonded to the second coating layer by a functional group present in the monolayer 221 or the polymer layer 222 included in the second coating layer 220.

The monomolecular film or the polymer film included in the second coating layer 220 of the micro disk 100 may be formed as a compound selected from a compound capable of chemically bonding or chemically adsorbing a specific material. The micro disk 100 coated with the first coating layer 210 and the second coating layer 220 on the surface may oscillate a laser beam having a constant wavelength value in the resonance mode. However, when a specific material is bonded or adsorbed to the monomolecular film 221 or the polymer film 222 included in the second coating layer 220, the micro disk 100 may have a wavelength value at which the wavelength value of the oscillation laser beam is changed. .

The micro-disc 100 is a wavelength of the changed wavelength in the resonance mode as a specific material is selectively bonded to the mono-molecular layer 221 or the polymer layer 222 included in the second coating layer 220 formed on the side of the micro-disc 100. It can be manufactured as a chemical sensor that can detect a specific material.

In addition, the micro disk laser according to an embodiment of the present invention may include a micro disk 100 and an excitation portion capable of exciting the edge of the micro disk 100, and is bound to the edge of the interface of the micro disk 100 The high quality whispering gallery mode can be oscillated in a single direction.

The micro disc laser can oscillate the whispering gallery mode having a high quality value in a single direction, so that light coupling is easy and light can be incident into the optical waveguide without any damage to the quality value. Therefore, when a chemical sensor is fabricated using a micro disk laser including the micro disk 100 having a high quality whispering gallery mode, it is possible to accurately detect a specific substance even in a low concentration gas environment.

The micro disk laser may include a plurality of micro disks 100. When the micro disk laser includes a plurality of micro disks 100, each of the plurality of micro disks 100 may have a first coating layer 210 and a second coating layer 220 on the side of the micro disk 100 under the same conditions. It is preferable that the first coating layer 210 and the second coating layer 220 formed on each of the plurality of micro disks 100 have the same thickness.

The excitation portion may excite the edge of the micro disk 100 by light or current. For example, as light or current is supplied through the upper or lower surface of the micro disk 100, the edge portion of the micro disk 100 may be excited, and the edge portion is inside the side of the micro disk, and the edge portion is the whispering. Not only the gallery mode but also the whispering gallery mode polygonal resonance mode can be formed.

When light or current is supplied to the micro disc 100, the whispering gallery mode having a high quality value may be oscillated at the edge of the micro disc 100 as an excitation portion. The whispering gallery mode may include a whispering gallery mode bound along an edge or boundary of the micro disc 100 or a resonance mode in the form of a whispering gallery mode bound along a periodic trajectory adjacent to the side of the micro disc 100. Can be.

In addition, the resonance mode bound along the periodic trajectory adjacent to the side of the micro-disc 100 may include a resonance mode in the form of a whispering gallery mode bound along a stable trajectory of period 4 or more or an unstable trajectory of period 4 or more. . In the case of the stable track less than period 4 and the unstable track less than period 4, the whispering gallery mode phenomenon disappears, and the whispering gallery mode bound along the periodic track adjacent to the side of the micro-disc 100 is stable in period 4 or more. It may include a resonance mode in the form of a whispering gallery mode that is bound along an orbit or an unstable orbit of period 4 or more.

The chemical sensor according to an embodiment of the present invention includes a measuring unit for measuring the oscillation wavelength value of the micro disk laser and the laser beam oscillated from the micro disk laser, the oscillation wavelength value of the laser beam measured by the measuring unit The specific substance can be detected using the change of.

The chemical sensor measures a slight change in the wavelength generated as the specific material is selectively bonded to the monolayer 221 or the polymer film 222 included in the second coating layer 220 of the micro disc 100, thereby determining the specific material. The type and concentration can be measured precisely.

The first coating layer 210 and the second coating layer 220 are formed on the side of the micro disk 100 included in the chemical sensor, and the micro disk 100 may oscillate a laser beam having a constant wavelength value in a resonance mode. have. When the chemical sensor is placed under a gas environment containing a specific material, the specific material present in the gas may be separated from the monomolecular film 221 or the polymer film 222 included in the second coating layer 220 of the micro disk 100 of the chemical sensor. It may be chemically adsorbed or bound. Accordingly, the existing wavelength value of the laser beam of the micro disc 100 may have a changed value.

The change in the oscillation wavelength value of the laser beam measured by the measuring unit of the chemical sensor is a change generated when a specific material is bonded to the second coating layer 220 of the micro disc 100. That is, the measurement unit may measure the changed oscillation wavelength value of the laser beam oscillated from the micro disc 100 by a specific material bonded to the second coating layer 220 of the micro disc 100. The measurement unit included in the chemical sensor may measure the wavelength value of the laser beam oscillated on the micro disk 100 before the specific material is combined with the wavelength value of the laser beam oscillated on the micro disk 100 after the specific material is combined. Can be.

The chemical sensor may include an operation unit driven in conjunction with the measurement unit, and the operation unit may calculate and provide a type of a specific substance and a concentration of the specific substance to the user by using the data value measured by the measurement unit.

For example, the chemical sensor may measure and calculate the degree of variation of the wavelength value of the micro disk due to the binding of a specific material to detect a specific material based on the calculated value. In addition, the degree of variation of the wavelength value can be processed in the form of a lookup table to measure the concentration of a specific substance, and the degree of variation of the wavelength value can be processed in a mathematical function or graph form to measure the concentration of the specific substance. In addition, the degree of variation of the wavelength value according to the measurement time may be processed in a mathematical function or graph form to more accurately measure the concentration of a specific substance.

Therefore, the chemical sensor may process the measured value in various forms and provide it to the user based on the variation degree of the wavelength value of the micro disk laser changed by the specific material.

In addition, the measuring unit may include a spectrometer. That is, the wavelength value of the micro disk laser changed by the specific material can be measured by using a spectrometer.

The change in wavelength value oscillating through the micro disc can be measured by optical beating with the wavelength oscillating in another micro disc or the wavelength generated by another high quality laser.

In addition, in order to improve the above-mentioned conventional problems, it is easy to use a micro resonator laser having a high quality value and oscillating in one direction. Recently, extremely high quality microresonator lasers oscillating in one direction have been developed. These resonators include a resonator laser consisting of four circles, a resonator laser consisting of three circles and a straight line, and an egg resonator laser. These resonators are extremely high in product quality, so the laser is fabricated and chemically treated to use the surface as a biosensor, and then used as a biosensor, which is an extremely high quality value, so that accurate measurement is possible and light comes out in one direction. Light can be incident on an optical waveguide without losing value. Creating biosensors in this way results in ultra-precision biosensors.

To make an ultra-precise biosensor, biomaterials must be selectively adsorbed to the side of the microdisc laser. One method for making a micro resonator laser is made of group III-V materials or semiconductors, or pigmented in various other polymer polymers, or using a general solid medium. For example, a III-V semiconductor material may be used as the semiconductor, and a GaAs series, InGaAsP, GaN series, or the like may be used as the III-V semiconductor material. In addition, as a solid medium, various solid laser media such as Nd: YAG, Nd: Glass, NdYVO ₄ , Sapphire doped with impurities, and ruby may be used.

As the polymer to which the dye is added, for example, a polymer to which a dye produced by adding a number of pigments such as Rhodamine 6G and Rhodamine B to a number of polymers such as polymethylmethacrylate (PMMA) can be used.

However, the kind of the polymer to which the above-described semiconductor, solid medium or pigment is added is merely an example for description, and does not limit the kind of the polymer to which the semiconductor, solid medium or dye is added.

Since the biomolecule cannot attach to the surface of such a laser medium, it must be chemically treated to have selectivity. Such chemical treatment methods have been developed in existing ring resonators. That is, SiO ₂ is coated on the side of the laser to attach the OH group, and then chemically surface-treated to attach the nucleic acid (DNA) or the antibody to the laser using a chemical reaction. Bacterial antigens bind to antibodies and, in the case of nucleic acids, to viral or bacterial nucleic acids.

In the measurement of wavelength variation using a biosensor, if the wavelength value after attaching the antibody is known, the wavelength variation can be measured when the antigen binds to the antibody. In addition, if the value of the wavelength after attaching the nucleic acid is known, the variation in the wavelength may be measured when the nucleic acids are combined. In other words, a predetermined substance can be detected (or sensed) by measuring the change in wavelength generated in the resonance mode of the micro disk constituting the biosensor. Since antigens and antibodies are selective, measuring the variation in wavelength when binding various antibodies after attaching various types of antibodies can confirm whether the antigens are bound, and thus can accurately diagnose diseases and the like. In the case of a nucleic acid, a laser oscillation wavelength is measured after attaching a nucleic acid of a specific bacterium or a virus-specific sequence artificially synthesized, and thereafter, a wavelength variation of a specific nucleic acid from a plurality of artificially synthesized nucleic acids by the virus or bacterial nucleic acid is measured. When the nucleic acid at this time is a virus or bacteria present, such a wavelength variation can be used to diagnose the disease.

In other words, an antigen or nucleic acid biomolecule is applied by chemical treatment to the surface of the microdisk resonator so that the biomolecule can adhere to the side of the unidirectional oscillation high quality microdisk laser, and the unknown biomolecule to be detected is the antigen or The presence of unknown biomolecules can be detected by measuring variations in wavelengths that occur upon binding to nucleic acid biomolecules.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

FIG. 6 is a view showing a state in which a micro disk side is coated with SiO ₂ , GaN, or the like according to an embodiment of the present invention, and FIG. 7 is a state in which an antibody is attached to the side after chemically treating the side of the micro disc. 8 is a view showing a state in which an artificial nucleic acid is attached to the side after chemically treating the micro disk side. 9 is a diagram showing an antigen-antibody reaction in which antigen binds to an antibody attached to the side of a micro disk, and FIG. 10 shows a reaction in which a nucleic acid of a virus or a bacterium binds to an artificial nucleic acid attached to the side of a micro disk. FIG. 61 is a schematic diagram of passing a biomaterial through a microfluidic to a biosensor comprising a resonator laser composed of microdisks. 12 is a schematic diagram showing a method for diagnosing a disease or the like by simultaneously measuring an antigen-antibody reaction and a reaction between nucleic acids.

As an embodiment of the present invention, a biosensor using a high quality micro disk may be provided. In a biosensor using a micro disk in which the whispering gallery mode has a high quality value, the micro disk has a single direction in which the whispering gallery mode has a stronger intensity of oscillation in one direction than an oscillation intensity in the other direction. Oscillated with a semiconductor, manufactured using a semiconductor, a solid medium or a polymer, the micro disc may be coated with a dielectric material on its side to enable chemical bonding with a virus, a bacterial antibody or a nucleic acid. In other words, micro discs can be fabricated using semiconductors, solid media or polymers. The Whispering Gallery mode, which is bound to the interface edge of the micro disc, may have a high quality value of 10 ⁴ or more.

In addition, the microdisc has a high quality factor in the whispering gallery mode bound to the edge, and can oscillate the whispering gallery mode in one direction, and the dielectric material (ex. Oxide or nitride) on the side of the microdisc. Etc.) By allowing the coating layer to be formed, the antibody or the prepared nucleic acid can be attached to the micro disk. In addition, a specific substance (eg, a virus, a bacterial antigen or a nucleic acid, etc.) may be selectively bound by an antibody or a prepared nucleic acid attached to a coating layer of a microdisk laser. The high-quality whispering gallery mode on the micro-disc can accurately detect specific substances even in low concentration samples. In addition, the biosensor accurately measures the type and concentration of a specific material by measuring a small change in the wavelength generated when the predetermined material is bound to an antibody or nucleic acid chemically bound to the coating surface of the micro disk. It can be measured.

The whispering gallery mode bound along the periodic trajectory adjacent to the side of the micro disk may include a resonance mode in the form of a whistle gallery mode bound along a stable trajectory of period 4 or more or an unstable trajectory of period 4 or more. In the case of stable tracks less than period 4 and unstable tracks less than period 4, the whispering gallery mode phenomenon is eliminated, so the whispering gallery mode, which is bound along the periodic track adjacent to the side of the microdisc, is stable or It may be desirable to include a resonance mode in the form of a whispering gallery mode bound along four or more unstable trajectories.

The dielectric material may be an oxide (eg SiO ₂₎ Etc.) or nitride (eg, GaN, SiN, etc.), and the antibody or nucleic acid can be attached to the micro disc via a chemical reaction on the side coated with the dielectric material. For example, the oxides are TiO ₂ , MgO, K ₂ O, Al ₂ O ₃ , Li ₂ O, Na ₂ O, Rb ₂ O, Cs ₂ O, BeO, CaO, SrO, BaO, B ₂ O ₃ , Ga ₂ O ₃ , In ₂ O ₃ , Ti ₂ O ₃ , SiO ₂ , GeO ₂ , SnO ₂ , PbO ₂ , P ₄ O ₁₀ , As ₂ O ₅ , Sb ₂ O ₅ , Bi ₂ O ₅ , SeO ₃ , TeO ₃ , PoO ₃ , I ₂ O ₇ and At ₂ O ₇ is an oxide selected from, the nitride is Li ₃ N, Na ₃ N, K ₃ N, Mg ₃ N ₂ , Be ₃ N ₂ , Ca ₃ N ₂ , Sr ₃ N ₂ , ScN, Fe ₂ N, Cu ₃ N, Zn ₃ N ₂ , (CN) ₂ . It may be a nitride selected from S ₄ N ₄ , Se ₄ N _4, GaN and SiN.

In addition, a predetermined antigen can be bound to the antibody through the antigen-antibody reaction in the micro disk, and whether or not the predetermined antigen is bound by the change of the wavelength of the resonance mode in the micro disk by the antigen bound to the antibody can be detected. Can be.

Predetermined nucleic acids can be bound through a reaction between nucleic acids in the micro disk, and whether or not the predetermined nucleic acids are bound by the change of the wavelength of the resonance mode in the micro disk by the bound nucleic acids.

The change in the wavelength value oscillating through this micro disk can be measured by optical beating with the wavelength oscillating in another micro disk or the wavelength oscillating in another high quality laser.

The micro-disc of the biosensor has an excitation device to oscillate a whispering gallery-shaped mode with a direction, and the biosensor may include a micro-disc laser using such a micro-disc.

In addition, the micro disk laser can use the dye laser which added the pigment | dye to a semiconductor, a solid medium, or a polymer.

The micro-disc laser can be excited with light or current in an area where the incident angle of the boundary surface of the resonator is larger than the critical angle in the Birkhoff coordinate system where the propagation of light inside the resonator is confused.

The biosensor may include a plurality of micro discs, each of which has a different antibody attached thereto, and a predetermined bio-antigen based on the degree of variation in wavelength during antigen-antibody reaction in each micro disc. Substances can be detected.

In addition, the biosensor may include a plurality of micro disks, and each of the plurality of micro disks has a different nucleic acid attached thereto. Certain biomaterials can be detected.

Predetermined biomaterials may be detected using a lookup table that represents the degree of variation in wavelength according to the reaction between different antibodies and the given biomaterial.

In addition, a lookup table showing a degree of variation in wavelength depending on the degree of attachment of a predetermined biomaterial, which is a detection target nucleic acid, to each different nucleic acid attached to each micro disk is used to detect a predetermined biomaterial. Can be.

The biosensor may include a plurality of micro disks, some of the micro disks have an antibody attached thereto, others have a nucleic acid attached thereto, and antigens to the antibody according to the change of wavelength in the micro disk by the antigen-antibody reaction. Is detected, whether or not the detection target nucleic acid is bound to the nucleic acid according to the change of the wavelength in the micro disk by the reaction between nucleic acids, whether the antigen binding and the binding of the nucleic acid can be detected at the same time. .

Further, the biomaterial to be detected based on predetermined data on the degree of variation in wavelength due to antigen-antibody reaction with the antibody attached to the micro disk or the degree of variation in wavelength due to internucleic acid reaction with the nucleic acid attached to the micro disk. This can be detected and this data can be in the form of a function or graph.

The micro disk may be a micro disk having a high quality value in the mode of whispering gallery in the chaotic resonator. Such a micro disk may be a micro disk in which a whispering gallery type mode oscillates with a stronger unidirectionality than oscillation intensity in one direction is stronger than oscillation in the other direction.

The whispering gallery type mode may be a mode in which the sine function of the angle of incidence of light in the Birkhoff coordinate system where the light propagates inside the micro disc resonator is confused.

In addition, the whispering gallery mode is constrained to a mariginally unstable periodic orbit where the angle of incidence of the boundary surface of the resonator is larger than the critical angle in the Birkhoff coordinate system where the light propagates in the micro disc resonator. It may be a mode.

The whispering gallery mode is a mode in which the incidence angle of the boundary surface of the resonator is in a region larger than the critical angle in the Birkhoff coordinate system where the light propagates in the micro disc resonator, or the light inside the resonator. In the Birkhoff coordinate system where the progression of chaos is confused, it may be a mode confined to an unstable orbit where the incidence angle of the boundary surface of the resonator exists in a region larger than the critical angle.

As an embodiment of the present invention, a biosensor using a micro disk laser having a high quality whispering gallery type mode oscillating strongly in one direction may be provided. Such a micro-disc laser may have an unintegratable shape such as a deformed circle, a shape consisting of four arcs, a shape consisting of three circles and one straight line, an ellipse shape such as an egg shape, and a shape in which a heart shape and an arc are combined. The non-integral shape means a shape in which the internal wave function cannot be obtained analytically by separating the Helmholtz equation in two dimensions. In order to use the micro-disc lasers as biosensors, GaN, SiN, or SiO ₂ may be coated on the side of the micro-disc first, and then the antibody or nucleic acid may be attached to the surface of the micro-disc through chemical treatment. At this time, a method known to attach the antibody or nucleic acid can be used. Whenever this adhesion occurs, there is a slight change in the refractive index of the microdisk, resulting in a variation in wavelength. Measuring the variation in these wavelengths estimates how many antibodies or nucleic acids are bound to the virus or bacterial antigen or viral or bacterial nucleic acid. You can do this and estimate the amount of virus or bacteria in the space. When a virus or bacterial antigen or nucleic acid is sent to a micro disc resonator laser, the antigen reacts with the antibody and the nucleic acid reacts with the nucleic acid, causing a different wavelength variation. Therefore, after attaching an antibody or artificial nucleic acid to a micro disk and measuring the wavelength of each resonator laser, a wavelength variation occurs when a virus or bacterial antigen or nucleic acid binds to an antibody or a nucleic acid, respectively. By measuring the variation of these wavelengths, a particular (eg, detection target) virus or bacterium can be identified. At this time, the antigen-antibody reaction occurs selectively, and in the reaction between nucleic acids, the binding occurs when the same nucleic acid, and thus, the specific disease can be diagnosed by identifying which laser has caused the wavelength variation. To this end, during the fabrication of ultra-high quality micro-disc laser, the surface of the SiO ₂ , GaN or SiN is coated by passivation after etching the micro disc. These coatings not only prevent leakage currents when injecting current into the laser, but also require various dielectric coatings for attachment of antibodies or artificial nucleic acids after chemical treatment.

In addition, attachment of the antibody or nucleic acid to the micro disc resonator may be easily attached to the antibody or artificial nucleic acid if a person of ordinary chemical knowledge.

The whispering galleries can be coated with a dielectric on the side of a high quality micro disk resonator laser that oscillates in a single direction, followed by chemical reaction to attach an antibody or nucleic acid. Subsequently, when a disease is to be diagnosed through an antigen-antibody reaction, antibodies corresponding to the diseases are attached to the side of each microdisk laser to a plurality of microdisk lasers, and then the microdisk lasers are microfluidic channel. In a virus or bacteria, and then flow through the microfluidic to produce an antigen-antibody reaction. At this time, the wavelength when the microfluidic is absent is measured, and then the variation of the wavelength generated when the antigen-antibody reaction occurs. These measurements are performed on all micro disk lasers, and micro disk lasers without antigen-antibody reactions do not produce wavelength variations, while micro disk lasers with antigen-antibody reactions may produce wavelength variations. In other words, it is possible to diagnose (detect) certain diseases, for example, to be detected, by measuring the variation in wavelengths resulting from the antigen-antibody reaction.

 When a disease is to be diagnosed through a nucleic acid reaction, a plurality of micro disk lasers are attached to the side of each micro disk laser by attaching nucleic acids artificially made of nucleic acids according to the unique nucleic acid sequence of each virus or bacterium. The microfluidic channel can be placed in a microfluidic channel, and a virus or bacteria can be treated to send a solvent from which nucleic acids are extracted to the microfluidic compound, and to generate a nucleic acid reaction. Since nucleic acids bind to each other by reacting between complementary nucleic acids, a variation in wavelength may occur only in those nucleic acids. In other words, since the bases of nucleic acids form hydrogen bonds with corresponding bases (e.g., A is T, G is C), only the complementary nucleic acid sequences react and bind, thus changing the wavelength of only the bound nucleic acids. Occurs. At this time, a solvent in which the nucleic acid is not extracted is flowed into the microfluidic, and the wavelength is measured, and then the variation of the wavelength generated when the nucleic acid reaction occurs. These measurements are performed on all micro disk lasers. Micro disc lasers without nucleic acid reactions do not produce wavelength variations, while micro disk lasers with nucleic acid reactions may cause wavelength variations. In other words, it is possible to diagnose (detect) certain diseases, for example, to be detected, by measuring the variation in wavelength caused by the nucleic acid reaction.

With regard to the method according to an embodiment of the present invention, the above description of the apparatus (eg, biosensor) may be applied. Therefore, with regard to the detection method of the biomaterial using the biosensor, the description of the same contents as those of the above-described apparatus is omitted.

One embodiment of the present invention can also be implemented in the form of a recording medium containing instructions executable by a computer, such as a program module executed by the computer. Computer-readable media includes both nonvolatile media, removable and non-removable media that can be accessed by a computer. In addition, computer readable media may include all computer storage media. Computer storage media includes both non-volatile, removable and non-removable media implemented in any method or technology for storage of information such as computer readable instructions, data structures, program modules or other data.

The foregoing description of the present invention is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is shown by the following claims rather than the above description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention. do.

Claims (27)

As a micro disc, The micro-disc has a high-quality whispering gallery mode, the whispering gallery mode oscillates with a direction, A first coating layer formed on a side of the micro disk by coating a dielectric; And And a second coating layer formed on the first coating layer and including one or more monomolecular films or polymer films. Wherein said dielectric is an oxide or nitride. According to claim 1, The micro disk is formed of a semiconductor, a solid medium or a polymer added with a pigment. According to claim 1, The oxide is TiO ₂ , MgO, K ₂ O, Al ₂ O ₃ , Li ₂ O, Na ₂ O, Rb ₂ O, Cs ₂ O, BeO, CaO, SrO, BaO, B ₂ O ₃ , Ga ₂ O ₃ , In ₂ O ₃ , Ti ₂ O ₃ , SiO ₂ , GeO ₂ , SnO ₂ , PbO ₂ , P ₄ O ₁₀ , As ₂ O ₅ , Sb ₂ O ₅ , Bi ₂ O ₅ , SeO ₃ , TeO ₃ , PoO ₃ , I ₂ O ₇ and At ₂ O ₇ is an oxide selected from, the nitride is Li ₃ N, Na ₃ N, K ₃ N, Mg ₃ N ₂ , Be ₃ N ₂ , Ca ₃ N ₂ , Sr ₃ N ₂ , ScN, Fe ₂ N, Cu ₃ N, Zn ₃ N ₂ , (CN) ₂ . A micro disk which is a nitride selected from S ₄ N ₄ , Se ₄ N _{4 ,} GaN and SiN. According to claim 1, The monomolecular film and the polymer film are attached to the first coating layer on the micro disk. According to claim 1, The monomolecular film and the polymer film may be selectively coupled to a specific material. The method of claim 5, As the specific material is selectively combined with the monomolecular film or the polymer film, the micro disk can detect the specific material by measuring a change in wavelength generated in the resonance mode of the micro disk. A micro disc laser comprising the micro disc of claim 1, An excitation portion capable of exciting an edge portion of the micro disc, A micro-disc laser that oscillates in one direction a high-quality whispering gallery mode bound to an edge of the micro-disc. The method of claim 7, wherein And the excitation portion excites the interface edge of the micro disk by light or current. The method of claim 7, wherein The whispering gallery mode includes a resonance mode in the form of a whispering gallery mode bound along a boundary of the micro disk or a whispering gallery mode bound along a periodic trajectory adjacent to a side of the micro disk. The method of claim 9, The whispering gallery mode bound along a periodic trajectory adjacent to the side of the micro disk includes a resonance mode in the form of a whispering gallery mode bound along a stable trajectory of period 4 or more or an unstable trajectory of period 4 or more. A micro disk laser according to claim 7; And And a measuring unit measuring an oscillation wavelength value of the laser beam oscillated from the micro disk laser. Chemical sensor for detecting a specific material by using the change in the oscillation wavelength value of the laser beam measured by the measuring unit. The method of claim 11, wherein The change in the oscillation wavelength value of the laser beam measured by the measuring unit is generated as the specific material is bonded to the second coating layer of the micro disk. The method of claim 11, wherein The measuring unit comprises a spectrometer. The method of claim 11, wherein Wherein the change in wavelength value oscillating through the micro disk is measured by optical beating with a wavelength oscillating in another micro disk or a wavelength oscillating in another high quality laser. A biosensor using a microdisc having a high quality value in a whispering gallery type mode, The micro-disc is oscillated with a unidirectionality in which the whispering gallery mode has a stronger intensity of oscillation in one direction than the intensity of oscillation in the other direction, and is manufactured using a semiconductor, a solid medium, or a polymer. The micro-disc is a biosensor, characterized in that the genetic material is coated on the side to enable chemical bonding with the virus, bacterial antibodies or nucleic acids. The method of claim 15, The dielectric material is an oxide or nitride, The biosensor, characterized in that the antibody or nucleic acid is attached to the micro disk through a chemical reaction on the side coated with the genetic material. The method of claim 16, The antigen-antibody reaction in the micro disk can bind a predetermined antigen to the antibody, And detecting the binding of the predetermined antigen according to the change of the wavelength of the resonance mode in the micro disk by the antigen bound to the antibody. The method of claim 16, Precise nucleic acids can be bound through a reaction between nucleic acids in the micro disk, Biosensor characterized in that the binding of the predetermined nucleic acid is detected according to the change of the wavelength of the resonance mode in the micro disk by the bound nucleic acid. The method of claim 15, The micro-disc includes a micro-disc laser using the micro-disc having an excitation device and oscillating a whispering gallery-shaped mode having a directivity. The method of claim 19, The micro-disc laser is formed as a dye laser in which a dye is added to a semiconductor, a solid medium, or a polymer. The method of claim 19, The micro-disc laser excites the micro-disc laser with light or current in a region where the angle of incidence of the boundary surface of the resonator is larger than a critical angle in a Birkhoff coordinate system where light propagates in the resonator. The method of claim 17, The biosensor may include a plurality of micro disks, Each of the plurality of micro disks has a different antibody attached to the biosensor, characterized in that the predetermined bio-material of the antigen is detected based on the degree of variation in the wavelength of the antigen-antibody reaction in each micro disk. The method of claim 18, The biosensor may include a plurality of micro disks, Each of the plurality of micro disks has a different nucleic acid attached to each other, and the biosensor which detects a predetermined biomaterial, which is a nucleic acid to be detected, is detected based on the degree of variation in wavelength during the reaction between nucleic acids in each micro disk. . The method of claim 22, The predetermined biomaterial is detected by using a lookup table indicating a degree of variation in wavelength according to the reaction between the respective different antibodies and the predetermined biomaterial. The method of claim 23, wherein The predetermined biomaterial is detected by using a lookup table showing a degree of variation in wavelength according to the degree of attachment of the detection target nucleic acid to the respective different nucleic acids. The method of claim 15, The biosensor may include a plurality of micro disks, Some of the plurality of micro disks have an antibody attached thereto, and others have a nucleic acid attached thereto, and whether or not the antigen is bound to the antibody according to the change of wavelength in the micro disk by the antigen-antibody reaction is detected. According to the change of the wavelength in the micro disk by the acid reaction, it is detected whether the nucleic acid to be detected is bound to the nucleic acid, Bio-sensor, characterized in that whether the binding of the antigen and the binding of the nucleic acid is detected at the same time. The method of claim 15, The biomaterial to be detected based on predetermined data on the degree of variation of the wavelength due to the antigen-antibody reaction with the antibody attached to the micro disk or the degree of the variation of the wavelength due to the nucleic acid reaction with the nucleic acid attached to the micro disk. Can be detected, The data is a biosensor, characterized in that the function or graph form.

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