WO2018035808A1 - Aquatic microorganism culture system - Google Patents

Abstract

An aquatic microorganism culture system, characterized in: comprising a supporting frame (3) having a plurality of layers of storage space (301); a plurality of transparent culture tubes (2) respectively provided at each layer of storage space (301), each culture tube (2) having a liquid inlet (202) and a liquid outlet (201); a liquid feed tube (12) in communication with the liquid inlet (202); a liquid discharge tube (11) in communication with the liquid outlet (201); an exchange device (4) for mixing and circulating a liquid containing aquatic microorganisms and discharged from the culture tubes (2), the liquid feed tube (12) and the liquid discharge tube (11) being respectively in communication with the exchange device (4); a water pump (5) having one end connected to the exchange device (4) and the other end in communication with the liquid feed tube (12); and multiple sets of light sources (1) for providing light intensity to the culture tubes (2), each layer of the storage space (301) of the supporting frame (3) being provided with one of the multiple sets of light sources (1). The invention solves a problem in which conventional aquatic microorganism production methods require occupation of large areas and high power consumption, have low productivity, are susceptible to contamination, and cannot be implemented for production all year round and in all weather conditions, thereby realizing a highly efficient and high-density commercial culturing of aquatic microorganisms.

Description

Aquatic microbial culture system Technical field

This invention relates to microbial production systems, and more particularly to aquatic microbial culture systems.

Background technique

The cultivation of aquatic microorganisms, especially microalgae, is usually carried out in open ponds or in sealed pipes, which are very inefficient and have a large footprint. More importantly, large-scale culture is highly susceptible to external chemical factors and foreign organisms, which affects the quality and stable production of algae products. In order to overcome these limitations, since the beginning of the 1980s, many companies have invested a lot of research and development energy to develop breeding equipment, but they can only be used in laboratories and small trial production. Moreover, the cost of sealed aquaculture systems is expensive, and subject to climate and ambient temperature, the efficiency and yield of aquaculture microorganisms using photosynthesis cannot be achieved at commercial scale.

Summary of the invention

In order to address one or more of the above problems, an aquatic microbial culture system is provided.

According to an aspect of the present invention, an aquatic microbial culture system is provided, comprising: a scaffold provided with a multi-layered space; a plurality of transparent culture pipes respectively disposed on each floor space, each culture pipe The inlet port and the outlet port are provided; the inlet pipe is connected to the inlet port; the outlet pipe is connected to the outlet port; and the exchange device is used for mixing and circulating the liquid containing the aquatic microorganisms from the culture pipe. The liquid pipe and the liquid outlet pipe are respectively connected with the exchange device; the water pump is used for conveying the liquid containing the aquatic microorganisms in the exchange device into the culture pipeline, one end of the water pump is connected with the exchange device, and the other end of the water pump is connected with the liquid inlet pipe; It is used to provide light intensity to the culture pipeline, and each set of space of each layer of the bracket is respectively provided with a set of the light sources. The utility model has the beneficial effects that: the invention adopts a plurality of transparent culture pipes respectively placed in the placement space of the support, and each layer is provided with a light source in the space for each layer, and the culture pipeline of each layer on the support is equipped with a corresponding set of light sources to form a culture. Unit, so that there are a plurality of breeding units on the support, and the aquatic microorganisms mixed liquid in the culture pipeline is cyclically mixed and exchanged for oxygen exchange through the exchange device, thereby forming a large-scale production system of aquatic microorganisms, and overcoming the occupation of conventional aquatic microorganisms The shortcomings of large area, low yield, easy pollution and inability to produce all-weather throughout the year have enabled all-round supply of light sources for breeding pipelines and improved photosynthesis efficiency. In addition, the aquatic microbial culture system of the present invention adopts a fully enclosed cycle continuous production and cultivation, which can greatly reduce the influence of external pollution, realize high-efficiency and high-density commercial aquaculture of aquatic microorganisms, and can overcome the climatic environment of the site. Conducive to the restrictions of aquatic microbial farming.

In some embodiments, a measurement monitoring device is also included, the measurement monitoring device being disposed within the exchange device and configured to monitor a growth environment of the aquatic microorganisms. The beneficial effect is that the measurement monitoring device can monitor the growth environment of the aquatic microorganisms, so that the aquatic microorganisms in the culture pipeline are in an optimal growth environment, and the microbial yield and yield are stably and greatly improved.

In some embodiments, the measurement monitoring device is a temperature, pH, dissolved oxygen aquatic monitor. The beneficial effect is that the aquatic monitor adopts a multi-probe system, which can simultaneously measure the temperature, dissolved oxygen and pH of the water body in the exchange device, and is convenient to use and accurate in monitoring.

In some embodiments, a variable frequency controller is provided on the water pump for regulating the rate at which water flows through the culture line. The beneficial effect is: a frequency conversion controller for regulating the circulation speed of the water flow in the culture pipeline to control the microorganisms in the culture pipeline to be harmless when flowing, and to ensure the improvement of the microbial yield.

In some embodiments, the exchange device is also provided with a gas injection device. The beneficial effect is that the addition of the gas injection device is beneficial to injecting the gas required for the microorganisms into the exchange device, and further beneficial to the improvement of the microbial yield.

In some embodiments, the exchange device is also provided with a sampling valve for collecting liquid within the exchange device. The utility model has the beneficial effects that the sampling valve is arranged under the exchange device through the first connecting pipe, and the liquid in the exchange device is conveniently collected into the harvesting tank for sampling, that is, when sampling is required, the sampling valve is opened to allow the liquid to flow out to the harvesting tank. Keep the operation of the pump, so that all the liquid in the pipeline flows out, the structure is simple and the operation is convenient.

In some embodiments, the light transmission rate of the culture pipeline is ≥ 85%, and the diameter of the culture pipeline is ≤ 30 mm. The beneficial effect is that the pipeline of this nature can effectively ensure the microbial yield and yield can be stably increased.

In some embodiments, the light source is a flat panel light source having a light intensity of > 300 micromoles per square meter per second. The utility model has the beneficial effects that the flat-type light source can ensure uniform illumination of the culture pipeline of each layer, and the light intensity of the light source is ≥300 μmol/m 2 /s, which ensures the microbial yield and the yield can be stably improved.

In some embodiments, the water pump is a low shear water pump. The beneficial effect is: low shear pump, so that when the system is running, the microorganisms are not harmed during the flow, and the microbial yield is effectively ensured.

In some embodiments, each culture conduit is placed in a placement space 301 in a helical manner. The beneficial effect is that, in the case that the size of the placement space is determined, each culture pipeline is placed in the placement space in a spiral manner, which can effectively increase the volume of each culture pipeline, and more effectively reduce the footprint of the entire system.

DRAWINGS

1 is a schematic structural view of an aquatic microbial culture system according to an embodiment of the present invention;

Figure 2 is a schematic view showing the structure of another angle of Figure 1;

Figure 3 is an enlarged view of a portion A in Figure 1;

Figure 4 is an enlarged view of a portion B of Figure 1;

Figure 5 is a schematic view showing the structure of a culture pipe and a light source.

detailed description

The invention will now be described in further detail with reference to Figures 1 to 5.

Referring to Figures 1 to 5, there is provided an aquatic microbial culture system comprising a stent 3 which may be metal welded. The holder 3 is provided with a multi-layered placement space 301 which can be formed by welding a plurality of spacers from the top to the bottom on the holder 3. A plurality of transparent culture pipes 2 are respectively disposed on each floor placement space 301, and each of the culture pipes 2 is provided with a liquid inlet 202 and a liquid outlet 201. The inlet pipe 12 is connected to the inlet port 202, and the outlet pipe 11 is connected to the outlet port 201. Transparent culture tubes 2 are commercially available. The exchange device 4 is for mixing and circulating the liquid containing the aquatic microorganisms from the culture pipe 2, and the inlet pipe 12 and the outlet pipe 11 are respectively connected to the exchange device 4, wherein the exchange device 4 can be a cylindrical shape. The sealed container has a diameter of 250 mm and a height of 500 mm, and the exchange device 4 is provided with an exhaust valve (not shown) for discharging the gas generated in the exchange device 4. a water pump 5 for conveying the liquid containing the aquatic microorganisms in the exchange device 4 to the culture pipe 2, one end of the water pump 5 is connected to the exchange device 4 through the first connection pipe 13, and the other end of the water pump 5 is connected to the liquid through the second connection pipe 14 The tube is connected in 12 ways. a plurality of sets of light sources 1 for providing light intensity to the culture pipe 2, and each set of space 301 of the support 3 is respectively provided with a set of the light sources 1, and each set of light sources 1 can correspondingly provide uniformity for the culture pipes 2 of the layer. , enough light intensity. The invention adopts a plurality of transparent culture pipes 2 respectively placed in the placement space 301 of the support 3, and each of the placement spaces 301 is provided with a light source 1, and each of the culture pipes 2 on the support 3 is provided with a corresponding set of light sources 1 Forming a culture unit, so that a plurality of culture units are present on the support 3, and the aquatic microorganism mixed liquid in the culture pipeline 2 is cyclically mixed and exchanged for oxygen exchange through the exchange device 4, thereby forming a large-scale production system of aquatic microorganisms, and the investment cost thereof It is lower than the conventional large-cell culture production system, because it is highly intensive production, easy to operate, easy to manage, and effectively reduce labor intensity. It overcomes the large area of conventional aquatic microbial production methods, high energy consumption, low yield, high cost, The shortcomings of serious pollution and the inability to produce all-weather throughout the year have enabled the full supply of light source 1 for the aquaculture pipeline 2, which has improved the photosynthesis efficiency. In addition, the aquatic microbial culture system of the invention adopts a fully enclosed circulating continuous production culture, can reduce the influence of external pollution factors, realize the high-efficiency and high-density commercial aquaculture of aquatic microorganisms, and can overcome the adverse effects on the site climate environment, such as aquatic microorganisms. Farming restrictions, insurance Certificate product quality.

Preferably, a measurement monitoring device 6 is also included, which is arranged in the switching device 4 and is used to monitor the growth environment of the aquatic microorganisms. The measurement monitoring device 6 can monitor the growth environment of the aquatic microorganisms, so that the aquatic microorganisms of the culture pipeline 2 are in an optimal growth environment, and the microbial yield and yield are stably and substantially improved.

Preferably, the measurement monitoring device 6 is a temperature, pH, dissolved oxygen aquatic monitor. The aquatic monitor can adopt a multi-probe system, which can simultaneously measure the temperature of the water body, the dissolved oxygen concentration and the pH in the exchange device 4, and is convenient to use and accurate in monitoring, so that the aquatic microorganisms of the culture pipeline 2 are in an optimal growth environment. Achieving a steady and substantial increase in microbial yield and yield. Aquatic monitors are available on the market, such as the YSI Aquarium Monitor.

Preferably, the water pump 5 is provided with a variable frequency controller 8 for regulating the speed at which the water flows in the culture line 2. The frequency conversion controller 8 is configured to adjust the speed of circulation of the water flow in the culture pipeline 2 to control the microorganisms in the culture pipeline 2 to be harmless when flowing, and to ensure the increase of the microbial yield.

Preferably, the exchange device 4 is also provided with a gas injection device 10. The addition of the gas injection device 10 facilitates the injection of the gas required for the microorganisms into the exchange device 4, and further facilitates the improvement of the microbial yield. For example, if the aquatic microbial culture system of the present invention is a microalgae, the gas injection can be performed. The device 10 injects carbon dioxide gas into the exchange device 4.

Preferably, the exchange device 4 is also provided with a sampling valve 7 for collecting the liquid in the exchange device 4. Further, a harvesting tank 9 is further included, and the exchange device 4 is provided with a sampling valve 7 on the first connecting pipe 13, and the harvesting tank 9 communicates with the first connecting pipe 13 through the sampling valve 7. A sampling valve 7 is disposed on the first connecting pipe 13 below the exchange device 4, so that the liquid in the exchange device 4 is collected into the harvesting tank 9 for sampling, that is, when sampling is required, the sampling valve 7 is opened for sampling, and the microbial cells are inspected. Growth conditions and nutrient consumption. In addition, the sampling valve 7 also facilitates the flow of the liquid into the harvesting tank 9 in the exchange device 4, keeps the operation of the water pump 5, and causes all the liquid in the pipeline to flow out, and has a simple structure and convenient operation.

Preferably, the light transmission rate of the culture pipe 2 is ≥ 85%, and the diameter of the culture pipe 2 is ≤ 30 mm. Pipes of this nature can effectively ensure that the microbial yield and yield can be steadily increased.

Preferably, the light source 1 is a flat panel light source, and the light source 1 has an intensity of ≥300 micromoles per square meter per second. The flat-type light source 1 can ensure uniform illumination of the culture pipe 2 of each layer, and the light intensity of the light source 1 is ≥300 μmol/m 2 /s, which can effectively ensure the microbial yield and the output can be stably improved, and the light intensity of the light source 1 can be Adjust according to the actual situation to prevent unnecessary waste.

Preferably, the water pump 5 is a low shear water pump 5. Since the microbial liquid in the culture pipeline 2 needs to be kept flowing, the low shear food grade water pump 5 can make the microorganisms not be harmed during the operation of the system, thereby effectively ensuring the increase of the microbial yield.

Preferably, each culture tube 2 is placed in the placement space 301 in a spiral or other flat manner. Inside. In the case where the size of the placement space 301 is determined, each culture pipe 2 is placed in the placement space 301 in a spiral or flat manner, which can effectively increase the volume of each culture pipe 2, and more effectively reduce the footprint of the entire system.

When the aquaculture system is used for aquaculture, first, a certain amount of water is injected into the exchange device 4, and then the water pump 5 is turned on. Next, according to the type of aquatic microorganism to be cultured, the corresponding medium is prepared and the medium is injected into the exchange device 4 to be uniformly stirred. Again, the liquid containing the aquatic microorganisms is injected into the exchange unit 4 at the concentration required for the culture, and then the frequency controller 8 is used to adjust the speed at which the water flows in the culture line 2 to a speed of 5 m/sec. Again, turn on the light source 1 of each layer and let it run. Thirdly, the temperature of the water in the exchange device 4, the dissolved oxygen concentration, and the pH are simultaneously measured by the aquatic monitor, so that the aquatic microorganisms of the culture pipe 2 are in an optimal growth environment. Again, 50 ml of water sample is taken daily through the sampling valve 7 to observe the growth of aquatic microorganisms, which can be harvested when the dry weight, cell maturity and absorbance (OD) reach the receiving standard. Finally, the sampling valve 7 is opened, the water in the exchange device 4 is discharged into the harvesting tank 9, the operation of the water pump 5 is maintained, and clean water is injected into the exchange device 4, so that all the water in the pipe flows out, thereby completing the harvesting work. . The aquatic microbial culture system of the invention adopts the closed-loop continuous production and cultivation, can reduce the influence of external pollution, realize the high-efficiency and high-density commercial aquaculture of aquatic microorganisms, and can overcome the restrictions on the aquatic environment and the like which are not conducive to aquatic microbial culture. .

What has been described above is only some embodiments of the invention. It will be apparent to those skilled in the art that various modifications and improvements can be made without departing from the spirit and scope of the invention.

Claims (10)

Aquatic microbial culture system, characterized in that it comprises The bracket (3) is provided with a multi-layered space (301). a plurality of transparent culture pipes (2) are respectively disposed on the space (301) of each floor, and each of the culture pipes (2) is provided with a liquid inlet (202) and a liquid outlet (201); a liquid inlet pipe (12) communicating with the liquid inlet (202); a liquid outlet pipe (11) communicating with the liquid outlet (201); An exchange device (4) for mixing and circulating a liquid containing aquatic microorganisms from the culture pipe (2), the inlet pipe (12) and the outlet pipe (11) respectively and said The switching device (4) is connected; a water pump (5) for conveying a liquid containing aquatic microorganisms in the exchange device (4) into the culture pipe (2), one end of the water pump (5) being connected to the exchange device (4) The other end of the water pump (5) is in communication with the inlet pipe (12); A plurality of sets of light sources (1) for providing light intensity to the culture pipe (2), and each of the floor space (301) of the support (3) is provided with a set of the light sources (1), respectively. The aquatic microbial culture system according to claim 1, characterized by further comprising a measurement monitoring device (6), said measurement monitoring device (6) being provided in said exchange device (4) for monitoring aquatic microorganisms Growing environment. The aquatic microbial culture system according to claim 2, characterized in that the measurement monitoring device (6) is a temperature, pH, dissolved oxygen aquatic monitor. The aquatic microbial culture system according to claim 1, characterized in that the water pump (5) is provided with a variable frequency controller (8) for regulating the speed at which the water flows in the culture pipe (2). The aquatic microbial culture system according to claim 1, characterized in that the exchange device (4) is further provided with a gas injection device (10). The aquatic microbial culture system according to claim 1, characterized in that the exchange device (4) is further provided with a sampling valve (7) for collecting the exchange device (4) Liquid. The aquatic microbial culture system according to any one of claims 1 to 6, wherein The light transmittance of the culture pipeline (2) is ≥ 85%, and the diameter of the culture pipeline (2) is ≤ 30 mm. The aquatic microbial culture system according to any one of claims 1 to 6, characterized in that the light source (1) is a flat light source, and the light source (1) has an intensity of ≥ 300 μmol/m 2 /s. The aquatic microbial culture system according to any one of claims 1 to 6, characterized in that the water pump (5) is a low shear water pump (5). The aquatic microbial culture system according to any one of claims 1 to 6, characterized in that each of the culture pipes (2) is placed in the space (301) in a spiral manner.

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