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WO2018018109A1 - Method for preparing an organic-mineral compound for agriculture, and product obtained - Google Patents

Method for preparing an organic-mineral compound for agriculture, and product obtained Download PDF

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Publication number
WO2018018109A1
WO2018018109A1 PCT/BR2016/050179 BR2016050179W WO2018018109A1 WO 2018018109 A1 WO2018018109 A1 WO 2018018109A1 BR 2016050179 W BR2016050179 W BR 2016050179W WO 2018018109 A1 WO2018018109 A1 WO 2018018109A1
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Prior art keywords
organic
mineral compound
agriculture
soil
nitrogen
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French (fr)
Portuguese (pt)
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Emílio Chaves MALAMAN
Ricardo Frateschi Pereira LIMA
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06Coating or dressing seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like

Definitions

  • the present invention belongs to the chemistry section, as it is a process of preparation of organic-mineral compound for use in agriculture.
  • Nitrogen is one of the most necessary elements for the proper development of plants, because it makes up the structure of nucleic acids and proteins, and is considered one of the most important nutrients (DOBBELAERE & OKON, 2007).
  • the importance of nitrogen is so important for plants that it becomes an extremely limiting element for the yield of grasses such as wheat and rice, and especially for those with high grain yields such as corn.
  • nitrogen fertilizer is a common practice and is responsible for raising the costs of agricultural production.
  • N 2 The main source of nitrogen is the atmosphere, which has approximately 78% N 2 (TAIZ & ZEIGER, 2004). However, this amount is not directly available to living beings and to be used because it needs to be fixed through industrial or natural processes (NUNES et al., 2003).
  • nitrogen is found in organic form, in organic matter; mineral, in soil solution and adsorbed to clays; and gaseous in the pores. Its main forms available are ammonium (NH 4 + ) and nitrate (NO 3 " ).
  • Nitrogen in its gaseous form is generally biologically fixed in other nitrogenous chemical species and is mobilized by some organisms, which employ it in protein and nucleic acid biosynthesis (NUNES et al., 2003).
  • Biological nitrogen fixation represents the main way to fix atmospheric N 2 in ammonium, revealing a key point of entry of molecular nitrogen into the biogeochemical nitrogen cycle (TAIZ & ZEIGER, 2004).
  • nitrogen provided by the biological fixation process is less prone to leaching and volatilization and is better employed as it is used in situ. In this sense, it can be noted that the biological process is a cheap, clean and sustainable alternative to the supply of nitrogen in commercial agriculture (HUERGO, 2006).
  • microorganisms As a fundamental tool of agriculture, there is the use of microorganisms, among the various types, some bacteria stand out; as asrizobacteria and those of the genus Azospirillum.
  • the use of microorganisms in agricultural practices is very relevant, since nitrogen fertilization represents an important part of the production costs of non-legume crops.
  • the benefits obtained by inoculating bacteria are varied and described as direct and indirect; among these benefits stand out as direct: the process of biological nitrogen fixation; phytohormones production, especially auxins and inorganic phosphate solubilization. Indirect benefits are related to systemic induction of disease resistance, biological control and production of iron-trapping organic compounds (DOBBELAERE & OKON, 2007).
  • Azospirilium brasilens is the class of plant growth promoting microorganisms because they are bacteria that contribute a number of benefits to the plant including biological nitrogen fixation and phytohormones production such as auxins and gibberellins, Plant growth hormones that act particularly on root development. Larger roots allow the plant greater absorption of water and nutrients, which makes it more resistant to water stress, and provides greater resistance to disease, as plants have better nutrient and water balance and are therefore healthier.
  • WO96 / 34840 entitled “Bacterialfertilizerandproductionprocess” describes a biofertilizer comprised of an aqueous suspension containing cells from the strains of the microorganisms Azotobactervinelandii and Azozpirillum brasilense; and the method for obtaining these microorganisms, which were specifically developed to obtain better nitrogen fixation and to potentiate alginate biosynthesis.
  • the biofertilizer contains in its formulation in addition to microorganisms, sucrose, PO 4 HK 2 , PO 4 H 2 K, SO 4 Mg, SO 4 Ca, SO 4 Fe, sodium molybdate and water.
  • PI 0722102-9 entitled “New Biological Fertilizer, Method for Obtaining It and Using It as a Plant Growth Stimulator” which relates to a product for biological fertilization consisting of a granulated formulation comprising two strains of bacteria of the genera Azospirillum and Pantoea, capable of fixing atmospheric nitrogen, solubilizing phosphates and other soil mineral nutrients and producing high amounts of plant growth stimulating substances.
  • the purpose of this application is to provide a biological fertilizer that exerts fertilization action and / or stimulation of plant growth.
  • the objective of the present invention is to provide an organic-mineral compound which, under conditions favorable to the proliferation of bacteria essential for agriculture, makes the fixation and mobilization of nitrogen, phosphorus and potassium more efficient for plants which are treated with it. . OF THE INVENTION
  • the present patent application relates to a process of preparation, with simple implementation steps, of a solid organic-mineral compound capable of providing a favorable environment for the proliferation of bacteria essential for agriculture, more precisely. Azospirillum brasilense bacteria, and thereby make the fixation and mobilization of nitrogen, phosphorus and potassium more efficient for plants that are treated with it.
  • the present invention has as its main advantages:
  • S Provide an organic-mineral compound that provides a favorable environment for the proliferation and development of Azospirillum brasilense, microorganisms essential for agriculture; Provide an organic-mineral compound that allows the fixation and mobilization of NPK (nitrogen, phosphorus and potassium), due to the good development of Azospirilium brasilense bacteria;
  • NPK nitrogen, phosphorus and potassium
  • ORGANIC-MINERAL COMPOUND PREPARATION PROCESS FOR AGRICULTURE AND OBTAINED PRODUCT (Fig. 1) takes place through simple production steps: Initially each of the components (C) is separated (E1) and then weighed (E2). ). For the preparation of the organic-mineral compound, between 55 and 65% of the total mass of the mixture, Azospirilium brasilense (1), available on the market, between 15 and 25% of the total mass of the mixture, conditioners are used. soil (2) composed of Humic and Fulvic Acids, also commercially available; between 15 and 25% of the total mass of the mixture, hydrogel (3), composed of CO 2 , H 2 O and KCI and, as the others components (C) available on the market.
  • the mixture (4) can be stored (E4) in vacuum plastic packaging for up to 04 months in a dry place at room temperature; or immediately applied (E5) to soil with seed, seedlings or pre-sprouted seedlings.
  • Ready-made organic-mineral compound (4) has acidic pH, between 4.5 and 5.5; and, it is applied (E5) directly to the soil with the seed or seedling, or pre-sprouted seedlings; replacing the traditional ways of preparing counterparts in concrete mixers or improvised equipment. Once incorporated into the soil, compound (4) promotes the development and proliferation of the bacteria (2) that compose it. This form of application of the compound (4) in the soil ensures that there will be no loss of biological material as it does not require preparation before application and its "activation" will be done after incorporation into the soil along with the seed.
  • compound (4) to the soil together with the seed at the time of planting offers a favorable biosystem for the growth and multiplication of bacteria, which favors the good plant development, due to the factors previously presented.
  • Compound (4) can still be applied to already planted and established plants to improve their development, rooting and health.

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  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

The subject matter relates to a method for preparing an organic-mineral compound for agriculture and to the product obtained. The method includes the steps of separating (E1), weighing (E2) and mixing (E3) the components (C) to produce the organic-mineral compound (4), which contains from 55 to 65% Azospirillum brasilense; from 15 to 25% soil-conditioners (2) composed of humic and fulvic acids; from 15 to 25% hydrogel (3) composed of CO2, H2O and KCl, the percentages being based on the total mass of the mixture. The organic-mineral compound is applied directly to the soil with seeds, seedlings or pre-planted seedlings (E5).

Description

PROCESSO DE PREPARAÇÃO DE COMPOSTO ORGÂNICO-MINERAL PARA AGRICULTURA E PRODUTO OBTIDO  PREPARATION FOR ORGANIC MINERAL COMPOUND FOR AGRICULTURE AND PRODUCT OBTAINED

BREVE DESCRIÇÃO BRIEF DESCRIPTION

[0001 ] Trata a presente solicitação de patentes de invenção de um inédito "PROCESSO DE PREPARAÇÃO DE COMPOSTO ORGÂNICO-MINERAL PARA AGRICULTURA E PRODUTO OBTIDO" que se refere a um processo de preparaçãoum composto orgânico-mineral que promove um ambiente favorável para a proliferação de bactérias essenciais para agricultura.  [0001] This patent application addresses an unprecedented "ORGANIC MINERAL COMPOUND PREPARATION PROCESS FOR AGRICULTURE AND OBTAINED PRODUCT" which relates to a process of preparation of an organic mineral compound that promotes a favorable environment for the proliferation of bacteria essential for agriculture.

CAMPO DE APLICAÇÃO APPLICATION FIELD

[0002] A presente invenção pertence à seção de química, por se tratar de um processo de preparaçãode composto orgânico-mineralpara ser utilizado na agricultura.  [0002] The present invention belongs to the chemistry section, as it is a process of preparation of organic-mineral compound for use in agriculture.

CONVENCIMENTO CONVENTION

[0003] A prática da adubação de plantas é muito antiga, existem registros datados de 08 mil anos a.C. sobre ela. A primeira fábrica de fertilizantes de que se tem notícias, surgiu por volta dos anos 1840, na Inglaterra, e desde então, cada vez mais, são observados avanços na agricultura e no que diz respeito aos fertilizantes, principalmente, motivados pela expansão populacional.  [0003] The practice of plant fertilization is very old, there are records dating from 08 thousand years BC about it. The first known fertilizer factory appeared around the 1840s in England, and since then, more and more progress has been made in agriculture and in fertilizer, mainly driven by population expansion.

[0004] Apesar da grande utilização de fertilização, cada vez mais, tem se pensado em seu impacto ambiental. Nesse sentido, nota-se o expressivo desenvolvimento de biotecnologias capazes de reduzir o uso de fertilizantes e agroquímicos, em busca de maior produção e qualidade. Despite the widespread use of fertilization, its environmental impact has been increasingly thought of. In this sense, there is the significant development of biotechnologies capable of reducing the use of fertilizers and agrochemicals, in search of greater production and quality.

[0005] Em geral, os compostos utilizados para fertilização na agricultura são a base de nitrogénio, fósforo e potássio, macronutrientes necessários ao bom desenvolvimento vegetal. O nitrogénio é um dos elementos necessário em maior quantidade para o desenvolvimento adequado das plantas, por ele compor a estrutura dos ácidos nucléicos e das proteínas, é considerado um dos nutrientes mais importantes (DOBBELAERE & OKON, 2007). A importância do nitrogénio é tamanha para os vegetais que, ele se torna um elemento extremamente limitante para a produtividade de gramíneas, como o trigo e o arroz, e principalmente, para as de altas produções de grãos como o milho. [0006] Como o nitrogénio é considerado essencial às plantas, o uso de fertilizante nitrogenado é uma prática comum e, responsável por elevar os custos da produção agrícola. Além de não ser uma prática muito sustentável, pois pode gerar danos ao ambiente, uma vez que a maior parte do produto aplicadanão é utilizada pelo vegetal, ficando depositada no solo e/ou águas apenas como resíduo. Em geral, a eficiência de utilização dos fertilizantes nitrogenados é de em média 50%, devido à ação da lixiviação, volatilização de amónia, desnitrificação, erosão e imobilização microbiana (REIS JÚNIOR et al., 2010). In general, the compounds used for fertilization in agriculture are based on nitrogen, phosphorus and potassium, macronutrients necessary for good plant development. Nitrogen is one of the most necessary elements for the proper development of plants, because it makes up the structure of nucleic acids and proteins, and is considered one of the most important nutrients (DOBBELAERE & OKON, 2007). The importance of nitrogen is so important for plants that it becomes an extremely limiting element for the yield of grasses such as wheat and rice, and especially for those with high grain yields such as corn. As nitrogen is considered essential to plants, the use of nitrogen fertilizer is a common practice and is responsible for raising the costs of agricultural production. In addition to not being a very sustainable practice, it can cause damage to the environment, since most of the applied product is not used by the vegetable, being deposited in soil and / or water only as waste. In general, the utilization efficiency of nitrogen fertilizers is on average 50%, due to the action of leaching, ammonia volatilization, denitrification, erosion and microbial immobilization (REIS JÚNIOR et al., 2010).

[0007] A principal fonte de nitrogénio é a atmosfera, que possui aproximadamente 78% de N2 (TAIZ & ZEIGER, 2004). Porém, esta quantidade não está diretamente disponível aos seres vivos e para ser utilizada, pois, necessita se fixada através de processos industriais ou naturais (NUNES et al., 2003). No solo, o nitrogénio é encontrado em forma orgânica, em matérias orgânicas; mineral, em solução do solo e adsorvido a argilas; e gasosa, nos poros. Suas principais formas disponíveis são amónio (NH4 +) e nitrato (NO3 "). [0007] The main source of nitrogen is the atmosphere, which has approximately 78% N 2 (TAIZ & ZEIGER, 2004). However, this amount is not directly available to living beings and to be used because it needs to be fixed through industrial or natural processes (NUNES et al., 2003). In soil, nitrogen is found in organic form, in organic matter; mineral, in soil solution and adsorbed to clays; and gaseous in the pores. Its main forms available are ammonium (NH 4 + ) and nitrate (NO 3 " ).

[0008] O nitrogénio em sua forma gasosa, em geral é fixado biologicamente em outras espécies químicas nitrogenadas, sendo mobilizada por alguns organismos, que o empregam na biossíntese de proteínas e ácidos nucléicos (NUNES et al., 2003). A fixação biológica de nitrogénio representa a principal forma de fixar N2 atmosférico em amónio, revelando-se um ponto chave de ingresso do nitrogénio molecular no ciclo biogeoquímico do nitrogénio (TAIZ & ZEIGER, 2004). Além disso, o nitrogénio fornecido pelo processo de fixação biológica é menos propenso a lixiviação e volatilização, sendo melhor empregado, uma vez que ele é utilizado in situ. Nesse sentido, é possível notar que o processo biológico é uma alternativa barata, limpa e sustentável para o fornecimento de nitrogénio na agricultura comercial (HUERGO, 2006). Nitrogen in its gaseous form is generally biologically fixed in other nitrogenous chemical species and is mobilized by some organisms, which employ it in protein and nucleic acid biosynthesis (NUNES et al., 2003). Biological nitrogen fixation represents the main way to fix atmospheric N 2 in ammonium, revealing a key point of entry of molecular nitrogen into the biogeochemical nitrogen cycle (TAIZ & ZEIGER, 2004). In addition, nitrogen provided by the biological fixation process is less prone to leaching and volatilization and is better employed as it is used in situ. In this sense, it can be noted that the biological process is a cheap, clean and sustainable alternative to the supply of nitrogen in commercial agriculture (HUERGO, 2006).

[0009] Como ferramenta fundamental da agricultura, tem-se a utilização de microrganismos, dentre os diversos tipos, destacam-se algumas bactérias; como asrizobactériase as do género Azospirillum. A utilização de microrganismos em práticas agrícolas é bastante relevante, visto que a adubação nitrogenada representa uma parcela importante dos custos de produção de culturas não-leguminosas.Os benefícios obtidos pela inoculação de bactérias, são variados e descritos como diretos e indiretos; dentre esses benefícios destacam-se como diretos: o processo de fixação biológica de nitrogénio; a produção de fitormônios, sobretudo auxinas e;a solubilização de fosfato inorgânico. Os benefícios indiretos estão relacionados à indução sistémica de resistência a doenças, controle biológico e produção de compostos orgânicos que captam ferro (DOBBELAERE & OKON, 2007). As a fundamental tool of agriculture, there is the use of microorganisms, among the various types, some bacteria stand out; as asrizobacteria and those of the genus Azospirillum. The use of microorganisms in agricultural practices is very relevant, since nitrogen fertilization represents an important part of the production costs of non-legume crops. The benefits obtained by inoculating bacteria are varied and described as direct and indirect; among these benefits stand out as direct: the process of biological nitrogen fixation; phytohormones production, especially auxins and inorganic phosphate solubilization. Indirect benefits are related to systemic induction of disease resistance, biological control and production of iron-trapping organic compounds (DOBBELAERE & OKON, 2007).

[0010] O emprego de insumos biológicos na agricultura tem se dado com maior frequência, a fim de substituir os insumos químicos industrializados, visando o fornecimento de nitrogénio às culturas com baixo custo económico e impacto ambiental reduzido. O potencial para uso na agricultura do género Azospirillum já foi bastante estudado, assim como, sua ecofisilogia (KEFALOGIANNI & AGGELIS, 2002). O género é representado por seis espécies diazotróficas, sendo uma das mais conhecidas ^Azospirílium brasilense. [0010] The use of biological inputs in agriculture has been more frequent in order to replace industrialized chemical inputs in order to supply nitrogen to crops with low economic cost and low environmental impact. The potential for use in Azospirillum agriculture has been extensively studied, as well as its ecophysilogy (KEFALOGIANNI & AGGELIS, 2002). The genus is represented by six diazotrophic species, one of the most known being Azospirilium brasilense.

[001 1 ] O Azospirílium brasilensecompõe a classe de microrganismos promotores do crescimento de planta, por se tratar de bactérias que contribuem com uma série de benefícios para a planta, incluindo a fixação biológica do nitrogénio e produção de fitormônios, como as auxinas e giberelinas, hormônios de crescimento de plantas que atuam particularmente no desenvolvimento das raízes. Raízes maiores permitem à planta maior absorçãode água e nutrientes, o que a torna mais resistente a estresses hídricos, além de proporcionar maior resistência a doenças, uma vez que as plantas apresentam melhor balanço de nutrientes e água e que, portanto, são mais saudáveis. [001 1] Azospirilium brasilensis the class of plant growth promoting microorganisms because they are bacteria that contribute a number of benefits to the plant including biological nitrogen fixation and phytohormones production such as auxins and gibberellins, Plant growth hormones that act particularly on root development. Larger roots allow the plant greater absorption of water and nutrients, which makes it more resistant to water stress, and provides greater resistance to disease, as plants have better nutrient and water balance and are therefore healthier.

[0012] Além dos benefícios para a plantaem se empregara Azospirílium brasilense, seu custo financeiro é muito inferior, quando comparado ao gasto com fertilizantes nitrogenados; e ainda existem as vantagens ambientais relacionadas ao seu uso, como a não poluição de rios e a não poluição de lençóisfreáticos, principalmente pelo nitrato, que é altamente poluente.  In addition to the benefits to the plant in using Azospirilium brasilense, its financial cost is much lower when compared to spent on nitrogen fertilizers; and there are still environmental advantages related to its use, such as no pollution of rivers and no pollution of groundwater, mainly by nitrate, which is highly polluting.

[0013] A partir do exposto, percebe-se a necessidade de um composto orgânico-mineral que possibilite condições favoráveis à proliferação de Azospirílium brasilense, e que a partir disso, torne mais eficiente a fixação de nitrogénio, fósforo e potássio para as plantas que, com ele forem tratadas. Nesse sentido, o composto orgânico-mineral, objeto deste pedido de patente é totalmente condizente com a atual demanda do mercado agrícola. From the foregoing, one realizes the need for an organic-mineral compound that allows favorable conditions for the proliferation of Azospirilium brasilense, and from that, make the fixation of nitrogen, phosphorus and potassium more efficient for plants that grow. , with him are treated. In this sense, the organic-mineral compound, object of this patent application is fully consistent with the current demand of the agricultural market.

ANTECEDENTES DA INVENÇÃO [0014] No atual estado da técnica, estão presentes diversos documentos que descrevem compostos orgânico-minerais e fertilizantes biológicos. Apesar disso, nenhuma das anterioridades encontradas antecipa completamente a proposta descrita por este pedido de patente. BACKGROUND OF THE INVENTION [0014] In the present state of the art, there are various documents describing organic-mineral compounds and biological fertilizers. Nevertheless, none of the foregoing found completely anticipates the proposal described by this patent application.

[0015] O documento de patente PI021 1920-0, intitulado "Preparações adequadas para tratamento do solo e de sementes de planta, processos para preparação das referidas preparações, para preparação de microrganismos, para tratamento de solo, para tratamento de sementes de plantas, bem como para melhorar e manter a estrutura do solo" presente no atual estado da técnica, refere-se a procedimentos para: preparação de produtos a partir dos microrganismos, a seguir especificados, ou a partir de uma mistura deles; criação de culturas dos microrganismos a serem usados; tratamento do solo e de plantas com os produtos que contenha pelo menos um dos microrganismo dentre Azoporillum brasilensessp. SW51 , Azotobactervinelandii spp. M657, Pseudomonasfluorescensvax. SW1 1 , Bacilluspolymyxa var. SW17, Bacillusmegaterium var. M326 e, ainda, aos produtos que se multiplicam e existam no ambiente em questão contendo os microrganismos anteriormente listados e sua produção. Nesse sentido, o objetivo de tal documento de patente é a proposição de produtos e procedimentos para o tratamento do solo com bactérias para o aperfeiçoamento do crescimento das plantas e aumento da eficiência na fabricação de produtos com essas finalidades.  Patent document PI021 1920-0 entitled "Preparations suitable for soil and plant seed treatment, processes for the preparation of said preparations, for the preparation of microorganisms, for soil treatment, for the treatment of plant seeds, as well as to improve and maintain soil structure "present in the state of the art, refers to procedures for: preparation of products from the following microorganisms or a mixture thereof; breeding of microorganisms to be used; treatment of soil and plants with products containing at least one of the microorganisms among Azoporillum brasilensessp. SW51, Azotobactervinelandii spp. M657, Pseudomonasfluorescensvax. SW11, Bacilluspolymyxa var. SW17, Bacillusmegaterium var. M326 and also to the products that multiply and exist in the environment in question containing the previously listed microorganisms and their production. In this sense, the objective of such patent document is the proposition of products and procedures for soil treatment with bacteria to improve plant growth and increase the efficiency in the manufacture of products for these purposes.

[0016] O pedido de patente WO96/34840, intitulado "Bacterialfertilizerandproductionprocess" descreve um biofertilizante compreendido por uma suspenção em meio aquoso que contém células de linhagens dos microrganismos Azotobactervinelandii e Azozpirillum brasilense; e ao método de obtenção desses microrganismos, que foram especificamente desenvolvidos para a obtenção de melhor fixação de nitrogénio e para a potencialização da biossíntese de alginatos. O biofertilizante contém em sua formulação além dos microrganismos, sacarose, PO4HK2, PO4H2K, SO4Mg, SO4Ca, SO4Fe, molibdato de sódio e água. WO96 / 34840, entitled "Bacterialfertilizerandproductionprocess" describes a biofertilizer comprised of an aqueous suspension containing cells from the strains of the microorganisms Azotobactervinelandii and Azozpirillum brasilense; and the method for obtaining these microorganisms, which were specifically developed to obtain better nitrogen fixation and to potentiate alginate biosynthesis. The biofertilizer contains in its formulation in addition to microorganisms, sucrose, PO 4 HK 2 , PO 4 H 2 K, SO 4 Mg, SO 4 Ca, SO 4 Fe, sodium molybdate and water.

[0017] Outro pedido de patente também presente no atual estado da técnica é o PI 0722102-9, intitulado "Novo fertilizante biológico, método para obtenção do mesmo e utilização deste como estimulador do crescimento vegetal" que refere-se a um produto para a fertilização biológica composto por uma formulação granulada que está compreendida por duas cepas de bactérias dos géneros Azospirillum e Pantoea, com capacidade para fixar nitrogénio atmosférico, solubilizar fosfatos além de outros nutrientes minerais do solo e produzir quantidades elevadas de substâncias estimuladoras do crescimento vegetal. O objetivo do referido pedido é proporcionar um fertilizante biológico que exerça ação de fertilização e/ou estimulação do crescimento vegetal. Another patent application also present in the present state of the art is PI 0722102-9, entitled "New Biological Fertilizer, Method for Obtaining It and Using It as a Plant Growth Stimulator" which relates to a product for biological fertilization consisting of a granulated formulation comprising two strains of bacteria of the genera Azospirillum and Pantoea, capable of fixing atmospheric nitrogen, solubilizing phosphates and other soil mineral nutrients and producing high amounts of plant growth stimulating substances. The purpose of this application is to provide a biological fertilizer that exerts fertilization action and / or stimulation of plant growth.

[0018] Apesar dasdiversas de anterioridades presentes no atual estado da técnica, observa-se que nenhuma das encontradas antecipa totalmente a proposta do presente pedido de patente. Pois, a mesma resolve problemas como: perda de colónias de microrganismos no momento da preparação e da aplicação dos biofertilizantes, uma vez que, em geral, são preparados em betoneiras no próprio local da aplicação. Além de resolver também, problemas com armazenamentodos biofertilizantes, que, em geral, requer temperatura ambiente, umidade e quantidade de nutrientes controladas, para que as colónias de bactérias se mantenham vivas. Despite the various priorities present in the present state of the art, it is noted that none of those found fully anticipates the proposal of the present patent application. Because it solves problems such as loss of colonies of microorganisms at the time of preparation and application of biofertilizers, since they are generally prepared in concrete mixers at the site of application. It also solves problems with biofertilizer storage, which generally requires room temperature, humidity, and the amount of controlled nutrients to keep the bacteria colonies alive.

OBJETIVO DA INVENÇÃO PURPOSE OF THE INVENTION

[0019] A presente invenção tem como objetivo proporcionar um composto orgânico-mineral que através das condições favoráveis à proliferação de bactérias essenciais à agricultura, torne mais eficiente a fixação e mobilização de nitrogénio, fósforo e potássio para as plantas que, com ele forem tratadas. DA INVENÇÃO  The objective of the present invention is to provide an organic-mineral compound which, under conditions favorable to the proliferation of bacteria essential for agriculture, makes the fixation and mobilization of nitrogen, phosphorus and potassium more efficient for plants which are treated with it. . OF THE INVENTION

[0020] O presente pedido de patente de invenção refere-se a um processo de preparação, com simples etapas de execução,de um composto orgânico- mineral sólido, capaz de propiciar um ambiente favorável para a proliferação de bactérias essenciais à agricultura, mais precisamente das bactérias Azospirillum brasilense, e que através disso, torne mais eficiente a fixação e mobilização de nitrogénio, fósforo e potássio para as plantas que, com ele forem tratadas.  The present patent application relates to a process of preparation, with simple implementation steps, of a solid organic-mineral compound capable of providing a favorable environment for the proliferation of bacteria essential for agriculture, more precisely. Azospirillum brasilense bacteria, and thereby make the fixation and mobilization of nitrogen, phosphorus and potassium more efficient for plants that are treated with it.

VANTAGENS DA INVENÇÃO  ADVANTAGES OF THE INVENTION

[0021 ] Em suma, a presente invenção apresenta como principais vantagens:  In summary, the present invention has as its main advantages:

S Proporcionar um composto orgânico-mineral que disponibilize um ambiente favorável para a proliferação e desenvolvimento das Azospirillum brasilense, microrganismos essenciais para a agricultura; Proporcionar um composto orgânico-mineral que permita a fixação e mobilização de NPK (nitrogénio, fósforo e potássio), devido ao bom desenvolvimento das bactérias Azospirílium brasilense; S Provide an organic-mineral compound that provides a favorable environment for the proliferation and development of Azospirillum brasilense, microorganisms essential for agriculture; Provide an organic-mineral compound that allows the fixation and mobilization of NPK (nitrogen, phosphorus and potassium), due to the good development of Azospirilium brasilense bacteria;

Proporcionar um composto orgânico-mineral que permita o aumento das radicelas das plantas, o que possibilita melhor nutrição e vigor da planta; Proporcionar um composto orgânico-mineral que reduza custos de produção com mão-de-obra, uma vez que sua aplicação é mais simples, o que representa ótima relação custo/benefício;  Provide an organic-mineral compound that allows the increase of plant roots, which enables better plant nutrition and vigor; Provide an organic-mineral compound that reduces labor costs, since its application is simpler, which represents an excellent cost / benefit ratio;

Proporcionar um composto orgânico-mineral que disponibilize macro e micronutrientes retidos no solo;  Provide an organic-mineral compound that provides soil-retained macro and micronutrients;

Proporcionar um composto orgânico-mineral que acelere processo de germinação das sementes, devido à umidade proveniente de seu hidrogel;  Provide an organic-mineral compound that accelerates the seed germination process due to moisture from its hydrogel;

Proporcionar um composto orgânico-mineral que reduza custos de produção com produtos químicos;  Provide an organic-mineral compound that reduces chemical production costs;

Proporcionar um composto orgânico-mineral que proporcione aumento na produtividade da safra;  Provide an organic-mineral compound that provides increased crop productivity;

Proporcionar um composto orgânico-mineral com maior prazo de validade.  Provide a longer term organic-mineral compound.

DESCRIÇÃO DA FIGURA DESCRIPTION OF THE FIGURE

[0022] A invenção será descrita em uma realização preferencial, assim, para melhor entendimento serão feitas referências ao fluxograma anexo.  The invention will be described in a preferred embodiment, thus, for better understanding reference will be made to the attached flow chart.

Figura 1 : Fluxograma do processo de preparação do composto orgânico- mineral  Figure 1: Flowchart of the organic-mineral compound preparation process

DESCRIÇÃO DETALHADA DA INVENÇÃO  DETAILED DESCRIPTION OF THE INVENTION

[0023] PROCESSO DE PREPARAÇÃO DE COMPOSTO ORGÂNICO- MINERAL PARA AGRICULTURA E PRODUTO OBTIDO(Fig. 1 ) se dá através de simples etapas de produção: Inicialmente cada um dos componentes (C) é separado (E1 ) e em seguida, pesado (E2). Para o preparo do composto orgânico-mineral, utiliza-se: entre 55 e 65%, da massa total da mistura, de Azospirílium brasilense (1 ), disponível no mercado;entre 15 e 25%, da massa total da mistura, de condicionadores de solo (2) compostos de Ácidos Húmicos e Fúlvicos, também disponíveis no mercado; entre 15 e 25%, da massa total da mistura, de hidrogel (3), composto de CO2, H2O e KCI e, como os demais componentes (C), disponível no mercado. Após a pesagem (E2) cada um deles é adicionado (E3) em uma batedeira industrial, para que haja obtenção de uma mistura (4) homogénea em estado sólido, uma vez que todos os componentes (C) são empregados nesse estado. Após obtida, a mistura (4) pode ser armazenada (E4) em embalagens plásticas à vácuo, por até 04 meses em local seco e à temperatura ambiente; ou imediatamente aplicada (E5) ao solo com semente, mudas ou mudas pré-brotadas. [0023] ORGANIC-MINERAL COMPOUND PREPARATION PROCESS FOR AGRICULTURE AND OBTAINED PRODUCT (Fig. 1) takes place through simple production steps: Initially each of the components (C) is separated (E1) and then weighed (E2). ). For the preparation of the organic-mineral compound, between 55 and 65% of the total mass of the mixture, Azospirilium brasilense (1), available on the market, between 15 and 25% of the total mass of the mixture, conditioners are used. soil (2) composed of Humic and Fulvic Acids, also commercially available; between 15 and 25% of the total mass of the mixture, hydrogel (3), composed of CO 2 , H 2 O and KCI and, as the others components (C) available on the market. After weighing (E2) each is added (E3) in an industrial mixer, so that a homogeneous solid state mixture (4) is obtained, since all components (C) are employed in this state. Once obtained, the mixture (4) can be stored (E4) in vacuum plastic packaging for up to 04 months in a dry place at room temperature; or immediately applied (E5) to soil with seed, seedlings or pre-sprouted seedlings.

[0024] O composto orgânico-mineral pronto (4) possui pH ácido, entre 4,5 e 5,5; e, é aplicado (E5) diretamente ao solo com a semente ou muda, ou ainda mudas pré-brotadas; substituindo as tradicionais formas de preparação de congéneres, em betoneiras ou equipamentos improvisados. Uma vez incorporado ao solo, o composto (4) propicia o desenvolvimento e a proliferação das bactérias (2) que o compõem. Essa forma de aplicação do composto (4) no solo, garante que não haverá perda de material biológico, uma vez que não requer preparação antes da aplicação e a sua "ativação" será feita após incorporação ao solo, juntamente com a semente.  Ready-made organic-mineral compound (4) has acidic pH, between 4.5 and 5.5; and, it is applied (E5) directly to the soil with the seed or seedling, or pre-sprouted seedlings; replacing the traditional ways of preparing counterparts in concrete mixers or improvised equipment. Once incorporated into the soil, compound (4) promotes the development and proliferation of the bacteria (2) that compose it. This form of application of the compound (4) in the soil ensures that there will be no loss of biological material as it does not require preparation before application and its "activation" will be done after incorporation into the soil along with the seed.

[0025] A aplicação do composto (4), no solo juntamente com a semente, no momento do plantio, oferece um biossistema favorável para o crescimento e multiplicação das bactérias, o que favorece o bom desenvolvimento vegetal, devido aos fatores anteriormente apresentados. O composto (4) ainda pode ser aplicado a plantas já plantadas e estabelecidas, para o melhorar seu desenvolvimento, enraizamento e saúde. The application of compound (4) to the soil together with the seed at the time of planting offers a favorable biosystem for the growth and multiplication of bacteria, which favors the good plant development, due to the factors previously presented. Compound (4) can still be applied to already planted and established plants to improve their development, rooting and health.

Claims

REIVINDICAÇÕES 1 . PROCESSO DE PREPARAÇÃO DE COMPOSTO ORGANICO-MINERAL PARA AGRICULTURA E PRODUTO OBTIDOcaracterizado porapresentar em sua composição:entre 55 e 65%, da massa total da mistura, de Azospirillum brasilense (1 ); entre 15 e 25%, da massa total da mistura, de condicionadores de solo (2) compostos de Ácidos Húmicos e Fúlvicos; entre 15 e 25%, da massa total da mistura, de hidrogel (3), composto de CO2, H2O e KCI; todos componentes (C) disponíveis no mercado. 1 . PROCESS FOR PREPARATION OF ORGANIC-MINERAL COMPOUND FOR AGRICULTURE AND PRODUCT OBTAINED BY PRESENTING IN THEIR COMPOSITION: Between 55 and 65%, of the total mass of the mixture, Azospirillum brasilense (1); between 15 and 25% of the total mass of the mixture of soil conditioners (2) composed of humic and fulvic acids; between 15 and 25% by weight of the total mixture of hydrogel (3), composed of CO 2 , H 2 O and KCl; all commercially available components (C). 2. PROCESSO DE PREPARAÇÃO DE COMPOSTO ORGANICO-MINERAL PARA AGRICULTURA E PRODUTO OBTIDO, de acordo com a reivindicação 1 ,seja preparada por simples etapas de separação (E1 ), pesagem (E2) e mistura (E3) dos componentes (C) disponíveis no mercadocaracterizado pelaobtenção composto orgânico-mineral (4) sólido. 2. ORGANIC MINERAL COMPOUND PREPARATION PROCESS FOR AGRICULTURE AND OBTAINED PRODUCT according to claim 1, is prepared by simple steps of separation (E1), weighing (E2) and mixing (E3) of the components available (C). market characterized by obtaining solid organic mineral compound (4). 3. PROCESSO DE PREPARAÇÃO DE COMPOSTO ORGÂNICO-MINERAL PARA AGRICULTURA E PRODUTO OBTIDO, de acordo com as reivindicações 1 e 2, onde ocomposto orgânico-mineral (4) seja caracterizada por sua aplicaçãodiretamente no solo com a semente ou muda, ou ainda, mudas pré-brotadas. Process for preparing organic mineral compost for agriculture and the product obtained according to claims 1 and 2, wherein the organic mineral compound (4) is characterized by its application directly to the soil with seed or seedling or seedlings. pre-sprouted.
PCT/BR2016/050179 2016-07-29 2016-07-29 Method for preparing an organic-mineral compound for agriculture, and product obtained Ceased WO2018018109A1 (en)

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Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1984001686A1 (en) * 1982-10-28 1984-05-10 Biotech General Corp Novel strains of azospirillum, methods of growing the strains, compositions containing them and use thereof as biofertilizer
WO2009091557A1 (en) * 2008-01-15 2009-07-23 Michigan State University Polymicrobial formulations for enhancing plant productivity
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Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1984001686A1 (en) * 1982-10-28 1984-05-10 Biotech General Corp Novel strains of azospirillum, methods of growing the strains, compositions containing them and use thereof as biofertilizer
WO2009091557A1 (en) * 2008-01-15 2009-07-23 Michigan State University Polymicrobial formulations for enhancing plant productivity
US20120057936A1 (en) * 2010-09-08 2012-03-08 Cook Robin J Methods and Composition for Improving Soil Quality

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