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WO2018009726A3 - Modification of dna polymerases for in vitro applications - Google Patents

Modification of dna polymerases for in vitro applications Download PDF

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Publication number
WO2018009726A3
WO2018009726A3 PCT/US2017/040991 US2017040991W WO2018009726A3 WO 2018009726 A3 WO2018009726 A3 WO 2018009726A3 US 2017040991 W US2017040991 W US 2017040991W WO 2018009726 A3 WO2018009726 A3 WO 2018009726A3
Authority
WO
WIPO (PCT)
Prior art keywords
modification
vitro applications
dna polymerases
pcr
vitro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2017/040991
Other languages
French (fr)
Other versions
WO2018009726A2 (en
Inventor
Mark Welch
Sridhar Govindarajan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dna20 Inc dba Atum
Original Assignee
Dna20 Inc dba Atum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna20 Inc dba Atum filed Critical Dna20 Inc dba Atum
Publication of WO2018009726A2 publication Critical patent/WO2018009726A2/en
Publication of WO2018009726A3 publication Critical patent/WO2018009726A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides DNA polymerase sequences for in vitro applications including PCR for creation of next generation sequencing libraries, and for reverse-transcriptase PCR.
PCT/US2017/040991 2016-07-06 2017-07-06 Modification of dna polymerases for in vitro applications Ceased WO2018009726A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662359059P 2016-07-06 2016-07-06
US62/359,059 2016-07-06

Publications (2)

Publication Number Publication Date
WO2018009726A2 WO2018009726A2 (en) 2018-01-11
WO2018009726A3 true WO2018009726A3 (en) 2018-02-22

Family

ID=60913163

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/040991 Ceased WO2018009726A2 (en) 2016-07-06 2017-07-06 Modification of dna polymerases for in vitro applications

Country Status (1)

Country Link
WO (1) WO2018009726A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022249133A1 (en) * 2021-05-28 2022-12-01 Erebus Enzymes Inc. Ultra-processive, stutter-resistant polymerase system

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010091203A2 (en) * 2009-02-04 2010-08-12 Lucigen Corporation Rna-and dna-copying enzymes
US20120083018A1 (en) * 2005-10-06 2012-04-05 Schoenfeld Thomas W Thermostable dna polymerases and methods of use

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120083018A1 (en) * 2005-10-06 2012-04-05 Schoenfeld Thomas W Thermostable dna polymerases and methods of use
WO2010091203A2 (en) * 2009-02-04 2010-08-12 Lucigen Corporation Rna-and dna-copying enzymes
US20130022980A1 (en) * 2009-02-04 2013-01-24 Lucigen Corporation Rna- and dna-copying enzymes

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CUSTAFSSON, C ET AL.: "Engineering Cene3 for Predictable Protein Expression", PROTEIN EXPRESSION AND PURIFICATION, vol. 83, no. 1, May 2012 (2012-05-01), pages 37 - 46, XP028413539 *
KUMAR, P ET AL.: "Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm", NATURE PROTOCOLS, vol. 4, no. 8, 25 June 2009 (2009-06-25), pages 1073 - 1082, XP055032238 *

Also Published As

Publication number Publication date
WO2018009726A2 (en) 2018-01-11

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