WO2018008864A1 - Cosmetic composition for wrinkle relief and anti-aging containing n-acetyl phytospingosine-1-phosphate - Google Patents

Abstract

Disclosed herein is a cosmetic product having anti-aging effects including wrinkle relief and elasticity enhancement, the cosmetic product containing N-acetyl phytosphingosine-1-phosphate (NAPS-1-P). The composition according to the present application can be effectively used in skin anti-aging through wrinkle relief and skin elasticity increase by multiplying fibroblasts and promoting collagen synthesis.

Description

Cosmetic composition for wrinkle improvement and anti-aging containing N-acetyl phytosphingosine-1-phosphate

The present invention relates to a cosmetic composition for anti-wrinkle and anti-aging, and more particularly to a cosmetic composition for anti-wrinkle and anti-aging containing N-acetyl phytosphingosine-1-phosphate.

Recently, the average life expectancy has increased due to the economic level and medical development, and interest in appearance has improved, and interest in improving wrinkles and preventing skin aging has naturally increased. Skin aging is a phenomenon caused by structural and functional changes in skin cells and tissues. It is classified into intrinsic aging and extrinsic aging.

Endogenous aging is an unavoidable aging phenomenon over time, which means skin wrinkles with genetic factors added. Exogenous aging causes skin wrinkles caused by environmental factors such as ultraviolet rays, gravity and polluted air. it means. Representative of exogenous aging is aging phenomenon observed on the skin of the face, back of the hand, neck, etc., which have been exposed to sunlight for a long time due to photoaging.

Anti-aging cosmetic research is being conducted by various approaches to improve wrinkles and inhibit aging, which mainly increases collagen production and suppresses degradation, promotes and inhibits elastin production, increases hyaluronic acid production, promotes aging cell regeneration, and activity. Oxygen removal, and the like. Recently, anti-aging cosmetics have been developed through the development of cellular signal transduction agents (Son et al (2005) J Invest Dermatol, 124: 1149-1161; Seite et al (2006) J Eur Acad Dermatol Venereol, 20: 980). -987; Ghersetich et al (1994) Int J Dermatol, 33: 661; Petropoules and Friguet (2005) Biochimica Biophtsica Acta, 1703: 261-266; Hazane et al (2005) J Photochem Photobiol, 79: 171-190).

Sphingolipids are known as representatives of cellular signal transduction. Sphingolipids are a constituent of cell membranes and also a variety of studies are being carried out as active molecules in vivo that regulate biological functions as secondary messengers. Among the sphingolipids, ceramides and sphingosine-1-phosphate (S1P) are known. Ceramide induces cell death, but S1P is a substance that promotes cell survival. In particular, S1P is a lipid metabolized from ceramide and is known to be involved in cell proliferation, differentiation, survival and neovascularization (Chung et al (2000) J Biol Chem, 275: 17229-17232; Spiegel and Milstien (2002) J). Biol Chem, 277: 25851-25854; Spiegel and Milstien (2003) Nat Rev Mol Cell Biol, 4: 397-407; Pyne and Pyne (2000) Biochem J, 349: 385-402).

Korean Patent No. 1003532 discloses a hair loss prevention, treatment or hair growth composition comprising N-cyclic phytosphingosine-1-phosphate.

Korean Patent Laid-Open No. 10-2014-0096042 relates to a cosmetic preparation comprising N-acyl-phytosphingosine and 1,2-pentanediol, which relates to cosmetic preparations that prevent sagging of skin or increase elasticity. The effect to make it is disclosed.

Republic of Korea Patent No. 1514970 discloses a composition for treating and preventing atopic or skin wounds comprising N-acetylphytosphingosine-1-phosphate.

However, none of the above patents discloses any effects such as skin aging including wrinkles based on fibroblast proliferation and collagen production promoting effects of N-acetyl phytosphingosine-1-phosphate and increased skin elasticity.

The present application is cosmetics for wrinkle improvement and anti-aging including N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) based on phytosphingosine-1-phosphate derivatives To provide a composition for.

In one aspect the present disclosure provides an anti-aging cosmetic composition comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic acceptable salt thereof.

The cosmetic composition according to the present invention has an effect on patent skin aging, and particularly shows an excellent effect on skin wrinkle improvement or skin elasticity enhancement.

The composition according to the present application may be prepared and applied in various formulations for skin application, and in one embodiment, a liposome formulation is prepared to increase skin absorption.

In another aspect the present application provides a cosmetic composition for promoting collagen production comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic acceptable salt thereof.

In another aspect, the present application provides an anti-wrinkle or improvement cosmetic composition comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic acceptable salt thereof.

Cosmetic compositions according to the invention can be prepared in various cosmetic forms, for example in the form of lotion, lotion, cream, essence, or ampoule depending on the site or method applied.

In the cosmetic composition according to the present application, the N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic acceptable salt thereof may be included in an amount of 0.01 to 100 ppm.

In the cosmetic composition according to the present invention, the N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic acceptable salt thereof may be provided included in the liposome, and the liposome is for example lecithin. It may include ingredients.

In the cosmetic composition according to the invention, the liposomes are nanoliposomes with an average diameter of about 100 to 200 nanometers, in particular 150 nanometers.

In another aspect the present disclosure also provides a kit for promoting the proliferation of fibroblasts comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or salts thereof, the fibroblasts proliferating, proliferating, in particular skin Proliferation of fibroblasts, and through this can be used for various purposes for the purpose of collagen enhancement.

In another aspect the present application provides the use of N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) for promoting the proliferation of fibroblasts.

In another aspect, the present application provides the use of N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) for enhancing collagen synthesis of cells.

In another aspect the present disclosure provides a method of promoting collagen synthesis in the skin of a subject comprising applying the cosmetic composition according to the present application to the skin of the subject in need of skin condition improvement through collagen synthesis.

In another aspect, the present invention provides a method for improving skin conditions, comprising applying the cosmetic composition to a skin of a subject in need of improvement.

The present application relates to a cosmetic composition for wrinkle improvement and anti-aging comprising N-acetyl phytosphingosine-1-phosphate (N-acetyl phytosphingosine-1-phosphate, NAPS-1-P), wherein the composition is a fiber Since it promotes the proliferation of blasts, increases collagen synthesis and enhances skin elasticity, it can be usefully used in cosmetics for wrinkle improvement and anti-aging.

In addition, the composition of the present application is economical because it uses N-acetyl phytosphingosine-1-phosphate, which is a substance to replace expensive sphingosine, which is a precursor of ceramide.

1 is a result of measuring the effect on the fibroblast proliferation of NAPS-1-P (N-acetyl phytosphingosine-1-phosphate) treatment group and the control group according to an embodiment of the present application.

Figure 2 is a result of measuring the effect on the proliferation of fibroblasts according to NAPS-1-P concentration according to an embodiment of the present application.

Figure 3 is the result of measuring the collagen production promoting effect of the concentration of NAPS-1-P according to an embodiment of the present application.

Figure 4 is a result showing the size distribution of the particles of the nanoliposomes comprising NAPS-1-P prepared according to an embodiment of the present application.

The present invention is based on the discovery that N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) promotes proliferation of fibroblasts and increases collagen synthesis.

Therefore, the present application relates to the use of N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) for promoting the proliferation of fibroblasts or for enhancing collagen synthesis or for improving skin condition.

In one embodiment the present application is directed to an anti-aging cosmetic composition comprising N-acetyl phytosphingosine-1-phosphate, a cosmetically acceptable salt or solvate thereof.

NAPS-1-P (N-acetylphytosphingosine-1-phosphate, Nacetyl-2-amino-3,4-dihydroxyoctadecyl hydrogen phosphate) included in the composition according to the present invention is represented by the following formula (1).

[Formula I]

The compounds according to the invention can be prepared using conventional knowledge known in the field of organic chemistry, for example (S. Li, WK Wilson, GJ Schroepfer, Chemical synthesis of D-ribo-phytosphingosine-1-phosphate, potential modulator of cellular processes.J. Lipid Res. 40: 117-125, 1999). Also refer to the Republic of Korea Patent No. 10-1003532 (composition for the prevention, treatment or hair growth of hair loss) or 10-1514970 (composition for the treatment and prevention of atopic or skin wounds) by the inventor.

Acceptable salts of the compounds according to the invention, or solvates thereof, may be appropriately prepared or selected by those skilled in the art of organic chemistry using knowledge known in the art.

Salts are physiologically acceptable and do not cause a conventional allergic or similar reaction upon administration to a human. The salts may be organic or inorganic salts, and include, but are not limited to, sodium, calcium and potassium salts. It may be prepared through a method or a process for preparing a salt known in the art. In addition, as the salt, an acid addition salt formed by free acid may be used. The free acid may be an organic acid or an inorganic acid. The organic acid is not limited thereto, citric acid, acetic acid, lactic acid, tartaric acid, maleic acid, fumaric acid, formic acid, propionic acid, oxalic acid, trifluoroacetic acid, benzoic acid, gluconic acid, metasulfonic acid, glycolic acid, succinic acid, 4-toluenesulfonic acid, Glutaric acid and aspartic acid. In addition, the inorganic acid includes, but is not limited to, hydrochloric acid, bromic acid, sulfuric acid and phosphoric acid.

In one embodiment according to the invention the pharmaceutically acceptable salts may be present as acid addition salts in which the compounds of formula (I) form salts with the free acid. In addition, the compounds of the formula (I) according to the present application may include not only pharmaceutically acceptable salts, but also all salts, hydrates, and solvates that can be prepared by conventional methods.

The compound of formula (I) may be stabilized by an anion paired with an ammonium cation in the compound, the anion may be any anion that is pharmaceutically acceptable and can be paired with an ammonium cation, for example iodide (I ^-), sulfonate (SO ₃ ^2-), chloride (Cl ^-), etc., but is not limited to this.

The compound according to the present invention or a composition comprising the same can be effectively used for anti-aging, that is, delaying aging, preventing aging through promoting the proliferation and collagen production of fibroblasts.

Aging in which the composition according to the present invention is effective refers to skin aging in particular. The aging is a phenomenon caused by structural and functional changes in skin cells and tissues, and is mainly caused by genetic factors such as endogenous aging and ultraviolet rays, This includes extrinsic aging caused by environmental factors such as gravity and polluted air. As a result of aging, that is, the aging phenomenon includes, but is not limited to, wrinkles on the skin, elasticity of the skin, and sagging of the skin. Exemplary exogenous aging is the aging phenomenon observed in the skin of the face, back of the hand, neck, etc., due to photoaging when exposed to sunlight for a long time.

Therefore, the compound according to the present application can be usefully used as a cosmetic composition for beauty.

As used herein, the term 'cosmetics' refers to an article used in the human body to brighten the human body to brighten the appearance of the body or to maintain or promote the health of the skin and hair, and refers to a slight action on the human body. By conventional meaning, it includes all lotions, creams, oils, cleaning products and functional cosmetics.

Functional cosmetics herein refers to the control of biological defense rhythms, disease prevention and recovery of cosmetic groups or cosmetic compositions that have added value to act and express the functions of cosmetics by using physical, biochemical and biotechnological techniques. It means cosmetics that are designed and processed to express the function of the gymnastics sufficiently to the living body, and in particular, mean anti-aging cosmetics.

Cosmetic compositions according to the invention may be provided as cosmetics, for example, creams, lotions, ampoules, skins, essences, soaps and the like, in other aspects such as basic cosmetic compositions (cosmetics, creams, essences, cleansing foams and cleansing water) Face wash, pack, body oil), color cosmetic composition (foundation, lipstick, mascara, makeup base) or in the form of a mask or pack.

The area to which the cosmetic composition according to the present invention can be applied is not limited to a specific part of the body, and may be applied to a wrinkled or wrinkled skin part such as a face, a neck part, a hand, etc. have.

Cosmetic compositions according to the invention can be administered at various sites of application, for example by systemic or topical, in particular topical, transdermal, or injection, in particular transdermal routes.

The cosmetic composition according to the present application is not particularly limited to other ingredients except for including the compound of the present invention as essential ingredients in the indicated ratios, and in the case of ordinary cosmetics such as cream, vegetable oils, emulsifiers, thickeners, flavorings, water, antioxidants And UV absorbers.

The content of the compound included in the composition according to the present application may vary depending on the specific use or formulation of the desired product, but may be included in about 0.001 to about 50% by weight in the total composition, but is not excluded from this range.

Cosmetic compositions comprising the compounds herein may include, in addition to the compounds, conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and / or flavors.

The cosmetic composition herein may comprise a water soluble base material comprising conjugated linoleic acid, linoleic acid, gamma-linoleic acid, unsaturated fatty acids including alpha-linoleic acid or propylene glycol.

Cosmetic compositions herein can also be prepared in any formulation conventionally prepared in the art suitable for administration to the skin for anti-aging, including, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, Powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, sprays, and the like, but are not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, mask, pack, spray or powder.

When the formulation of the present composition is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. Can be.

If the formulation of the composition is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, especially in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

Cosmetic compositions according to the invention may further comprise a cosmetically / dermatologically acceptable carrier. “Cosmetic / dermatologically acceptable” means suitable for use in contact with tissue (eg skin or hair) without excessive toxicity, incompatibility, instability, irritation, allergic reactions, and the like.

When the formulation of the composition is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol, glycerol aliphatic ester, polyethylene glycol or sorbitan may be used.

When the formulation of the composition is a suspension, the carrier component is water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, and microcrystals. Castle cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present composition is a surfactant-containing cleansing, as a carrier component, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolium derivative, a methyltaurate, a sarcosinate, a fatty acid amide ether Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used. According to a preferred dosage type, the compositions of the present invention may comprise at least one pharmaceutically acceptable excipient, in particular dermatologically acceptable excipients.

In addition, in the cosmetic composition for skin of each formulation, other components than the above essential ingredients can be selected and blended by those skilled in the art without difficulty according to other skin compositions or purposes of use.

Since the composition of the present invention has little toxicity and side effects, it can be used with confidence even for prolonged administration for prophylactic purposes.

In one embodiment according to the present disclosure the compound NAPS-1-P according to the present disclosure may be prepared as a nanoliposomal formulation. The nanoliposomes are in the form of conventional liposomes, and the average particle diameter of liposomes, particularly nanoliposomes, of 100-200 nm is 140-160 nm. The liposomes take the form of microcapsules or spheres and may serve to deliver the active ingredients directly to the skin. In the present invention, by containing NAPS-1-P in the nanoliposomes, the NAPS-1-P can be better delivered and absorbed to the skin.

The nanoliposomes can be prepared through various methods known in the art. In one embodiment of the present application is manufactured by Sony. Nanoliposomes according to the present invention are in the form of conventional, shelled liposomes. The liposomes take the form of fine capsules or spheres, and may serve to directly transfer the ingredients used in skin care to the skin.

In one embodiment NAPS-1-P according to the present application is provided contained in liposomes formed with lecithin. Lecithin is an amphiphilic phospholipid consisting of fatty acids such as choline, glycerol or glycofatty acid or triglycerides as phosphoric acid and lipid components. Phosphatidylcholine, phosphadidylethanolamine, and / or phosphatidyl inositol, and the like, and are the main constituents of biological membranes, which can be extracted from various animals, plants, yeasts, etc., and are commercially available.

In another aspect, the present invention also relates to a kit for promoting proliferation or enhancing collagen synthesis of fibroblasts comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or salts thereof.

The active ingredient included in the kit according to the present disclosure may refer to the aforementioned, and may further include additional ingredients, usage, etc. for the desired effect in the in vitro. Kits according to the present invention can be used in a variety of ways that promote the growth of fibroblasts or promote collagen production through it.

As mentioned above, the cosmetic composition comprising the active ingredient according to the present application may improve the condition of the skin such as wrinkle improvement or elasticity through fibroblast proliferation and collagen synthesis.

Thus, in another aspect, the present invention provides a method for promoting collagen synthesis in the skin of a subject comprising applying the cosmetic composition according to the present application to the skin of a subject in need of improving the skin condition through collagen synthesis or promoting fibroblast proliferation It is about.

In addition, the present invention provides a method for improving skin condition, comprising applying the cosmetic composition according to another embodiment to the skin of a subject in need of improvement of skin condition.

The cosmetic composition used in the method according to the invention can be referred to above. Cosmetic compositions according to the present application can be applied to all or part of the whole body, including various areas that need improvement of the skin, such as the face, neck, hands, arms, legs and the like. Cosmetics according to the present application may be applied in an amount sufficient to produce the desired effect, and those skilled in the art will be able to determine the appropriate amount in view of the disclosure herein and the knowledge in the art.

Hereinafter, examples are provided to help understand the present invention. However, the following examples are provided only to more easily understand the present invention, and the present invention is not limited to the following examples.

Example

Experimental Materials and Methods

Preparation of NAPS-1-P

NAPS-1-P (N-acetyl phytosphingosine-1-phosphate) was prepared and used according to the method described in Korean Patent No. 1003532.

Proliferation of Fibroblasts

Human fibroblasts (CCD-9865K, Korea Cell Line Bank) were incubated with DME (Dulbecco's Modified Eagle Medium) medium containing 10% fetal calf serum and 1% penicillin / streptomycin every 3 days with medium. The culture environment was incubated in a 5% CO ₂ incubator maintained at 37 ° C. The proliferative effect of fibroblasts was measured using MTT (3- (4,5-dimethylthiazol-2yl) -2,5-diphenyl-2H-tetrazolium bromide) assay (Sigma) according to the manufacturer's method, and the absorbance at 595 nm was measured. The cell proliferation effect was observed.

Collagen Synthesis Effect Measurement

Human fibroblasts were cultured every 3 days in DMEM medium containing 10% fetal calf serum and 1% penicillin / streptomycin to see the effect of promoting collagen synthesis to measure the wrinkle improvement effect of the skin. The culture environment was incubated in a 5% CO ₂ incubator maintained at 37 ° C. After incubation for 24 hours, the solution was diluted with culture medium (dissolved in dimethyl sulfoxide (DMSO) at 10000 ppm, diluted with medium, and diluted to 0.1-10 ppm), followed by incubation for 24 hours. Subsequently, the culture medium was taken, and collagen amount was measured using a procollagen type I C-peptide EIA kit (Takara Bio Inc, MK 101) according to the manufacturer's method. The absorbance was measured at 450 nm to calculate the amount of collagen.

Preparation of NAPS-1-P-containing Nanoliposomes

Nanoliposomes to be applied to the skin was prepared by the sonication method as described below, the composition of the nanoliposomes are shown in Table 1 below. Specifically, lecithin was first dispersed in ethanol or butylene glycol and hexanediol for the preparation of nanoliposomes. NAPS-1-P and arginine were mixed and then dissolved by warming with water. The prepared NAPS-1-P solution was stirred vigorously while slowly added to the lecithin solution. After stirring for 30 minutes, it was sonicated with a bath type sonicator for 60 minutes. The particle size of the prepared nanoliposomes was measured according to the manufacturer's method using laser light scattering (Photal Otsuka Electronics, Japan). The particle size of the nanoliposomes is shown by volume distribution.

The nanoliposomes made of the following compositions 1 and 2 were made in a very stable state, and the particle size of the nanoliposomes was measured, and there was no difference in particle size in the two compositions. As shown in FIG. 3, the average particle size of the two compositions was found to be about 150 nm.

Table 1 Composition of Nanoliposomes Containing NAPS-1-P

Skin elasticity measurement

Skin firmness instrument evaluation was determined using the Cutometer MPA580 (Courage and Khazaka, Germany) measuring equipment according to the manufacturer's method. The measurement method was a non-invasive method of 2mm diameter probe connected to the device in close contact with the skin. The unit of measurement is expressed in arbitrary units AU (arbitary unit). The measurements were taken before and two and four weeks after application to the skin. Skin elasticity improvement rate was calculated as follows.

Improvement rate (%) = (measured value after applying cosmetics-average value measured before using cosmetics) / average value measured before using cosmetics X 100

Cosmetics (ampoules) containing NAPS-1-P nanoliposomes for measuring skin elasticity were prepared. The composition of the ampoule cosmetics is shown in Table 2 below.

Table 2 Composition of ampoule cosmetics containing compositions 1 and 2

Example 1 Fibroblast Proliferation Promoting Effect by NAPS-1-P

Human fibroblasts are cells that synthesize collagen, which promotes the proliferation of fibroblasts and can measure the effect of improving wrinkles. Addition of NAPS-1-P in the culture of fibroblasts was shown to promote the proliferation of fibroblasts (FIG. 1). As a result of measuring the fibroblast proliferation effect of N-acetyl phytosphingosine (NAPS) and NAPS-1-P precursors of NAPS-1-P, NAPS does not promote fibroblast proliferation. On the contrary, the result of promoting death was obtained, but NAPS-1-P resulted in promoting fibroblast proliferation (Table 3, FIG. 2). Therefore, NAPS-1-P, unlike NAPS, promotes the proliferation of fibroblasts, indicating that it can be usefully used in anti-aging cosmetics including wrinkle improvement.

[Table 3] Fibroblast Survival Comparison of NAPS-1-P and NAPS

Example 2 Collagen Production Promoting Effect by NAPS-1-P

In order to improve wrinkles and improve elasticity, it is important to promote collagen synthesis and inhibit degradation. Therefore, for anti-aging cosmetics including wrinkle improvement and elasticity enhancement, collagen synthesis and collagen degradation inhibitory effects were measured. When NAPS-1-P was added in the culture of fibroblasts, the growth of fibroblasts and the amount of collagen synthesized in the fibroblasts were measured. Increasing the concentration of NAPS-1-P was shown to increase the production of collagen as well as the proliferation of fibroblasts (Fig. 3). The results indicate that the synthesis of collagen is promoted by NAPS-1-P, which increases collagen production. As a result, NAPS-1-P has an effect of improving wrinkles and increasing elasticity. It is useful for anti-aging cosmetics including wrinkle improvement and elasticity.

Example 3. Skin elasticity enhancing effect

As described in the above experimental method, ampoule cosmetics containing the nanoliposomes of the compositions 1 and 2 were prepared and applied to the skin, and then the change in skin elasticity was measured. Subjects were asked to apply ampoule 1 to the right face and ampoule 2 to the left face twice a day for 4 weeks for 20 people in their 30s to 50s (mean age 43.2) who are concerned about skin aging. Skin elasticity was measured before and 2 weeks and 4 weeks after application and the results are shown in Table 4. As a result, the skin elasticity tends to increase with the application time and shows higher skin elasticity than before application. There was no difference in skin elasticity between the two ampoule cosmetics of different compositions. These results indicate that NAPS-1-P is a material that can be usefully used in anti-aging cosmetics including wrinkle improvement and elasticity improvement.

[Table 4] Change of skin elasticity of ampoule cosmetics containing NAPS-1-P

As described above, the composition containing NAPS-1-P has the effect of increasing the proliferation of fibroblasts and also increasing collagen synthesis. The application of ampoules comprising the composition according to the present application directly to the skin also showed an increase in skin elasticity. Therefore, the composition containing NAPS-1-P of the present application can be usefully used in anti-aging cosmetics including wrinkle improvement and elasticity improvement.

Although the exemplary embodiments of the present application have been described in detail above, the scope of the present application is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concepts of the present invention defined in the following claims are also provided. It belongs to.

All technical terms used in the present invention, unless defined otherwise, are used in the meaning as commonly understood by those skilled in the art in the related field of the present invention. The contents of all publications described herein by reference are incorporated into the present invention.

Claims (12)

An anti-aging cosmetic composition comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic acceptable salt thereof and a cosmetically acceptable carrier. The anti-aging cosmetic composition of claim 1, wherein the anti-aging is skin wrinkle improvement or skin elasticity increase. The anti-aging cosmetic composition of claim 1, wherein the composition is a liposome formulation for skin application. The anti-aging cosmetic composition of claim 3, wherein the shell of the liposome comprises lecithin, and the average diameter of the liposome is about 100 to 200 nanometers. Cosmetic composition for promoting collagen production comprising N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or an acceptable salt for cosmetics and an acceptable carrier for cosmetics. N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetic composition for inhibiting or improving wrinkles comprising a salt acceptable for cosmetics and an acceptable carrier for cosmetics. The composition of claim 1, wherein the cosmetic composition is in the form of a lotion, lotion, cream, essence, or ampoule. The method according to any one of claims 1 to 6, wherein the N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) or a cosmetically acceptable salt thereof is included in an amount of 0.01 to 100 ppm. , Composition. Use for promoting the proliferation of fibroblasts of N-acetyl phytosphingosine-1-phosphate (NAPS-1-P). Use of N-acetyl phytosphingosine-1-phosphate (NAPS-1-P) for enhancing collagen synthesis of cells. A method of promoting collagen synthesis in the skin of a subject, comprising applying the cosmetic composition of claim 1 to the skin of the subject. A method for improving skin conditions, including wrinkle improvement or elasticity enhancement, comprising applying the cosmetic composition of claim 1 to the skin of a subject in need of improvement of skin condition.

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