WO2017185290A1 - Method for preparing lycium ruthenicum extract - Google Patents

Abstract

A method for preparing a lycium ruthenicum extract comprises the following steps: pulverizing lycium ruthenicum fruits, adding water into the pulverized lycium ruthenicum fruits to mix evenly, putting the mixture in a sealed state for 1 to 3 days, air-drying the mixture in an indoor temperature, carrying ultrasonic extraction by sequentially using hydrochloric acid, citric acid and noxalic acid, the extraction temperature ranging from 65ºC to 75ºC, and the time of a single ultrasonic extraction ranging from 1 h to 2 h; merging the extracted liquids; carrying filtering when the merged extracted liquids are hot, and feeding the product into a centrifugal machine to carry out centrifugation, the rotation speed of the centrifugal machine being 3000 r/min to 5000 r/min, and the centrifugation time ranging from 24 min to 45 min; collecting the supernatant; and feeding the supernatant into a chromatographic column filled with macroporous resin to perform adsorption; and sequentially carrying out elution by sequentially adding water and elution liquid; and feeding the product into a rotary evaporator for concentration, the concentration temperature ranging from 55ºC to 65ºC; and carrying out drying to obtain the lycium ruthenicum extract. The preparation method has a simple process, low costs and very high extraction efficiency.

Description

Method for preparing black scorpion extract Technical field

The invention relates to the technical field of black peony extract, in particular to a preparation method of black peony extract.

Background technique

Black carp is a wild Solanaceae plant, which is light and vigorous under full light conditions. It grows weakly under shade conditions, with few flowers and fruits, and is highly adaptable. It is in saline-alkali wasteland, salinized sandy land, salt lake shore, It grows well in various salinized soils on both sides of the channel, river beach, etc. It can withstand the high temperature of 38.5 °C, and has strong cold resistance. It has no freezing damage under the low temperature of -25.6 °C, and can still grow drought-tolerant in desert, mature purple. Black, non-toxic and sweet, with eyesight and benefit, anti-fatigue, beauty, and tumor inhibition. The content of sodium in black sorghum is equivalent to that of common cockroaches, but the content of elements such as calcium, magnesium, copper, zinc and iron is much higher than the average content of corresponding elements in common strontium. The content of potassium and manganese is much lower than the average value, and the content of nickel is high. It is higher than cereals, cured meats and vegetables. In particular, the content of lead and cadmium in black sorghum is much lower than that of 0.5-3.0 μg/g in general plants. At present, the effective material extraction technology suitable for large-scale popularization and application of black fruit carp has low extraction efficiency, high extraction cost, and complicated process.

Summary of the invention

The invention provides a preparation method of black scorpion extract, which has simple process, low cost and high extraction efficiency, and has certain promotion and application value.

The preparation method of the black scorpion extract proposed by the invention comprises the following steps:

S1, weigh 40-70 parts of black sorghum fruit by weight, smash it into 20-45 parts of water, mix it evenly, seal it for 1-3 days, air-dry at room temperature, then use 100-150 parts of concentration 1.5-3wt% Ultrasonic extraction of hydrochloric acid, 100-140 parts of citric acid, 120-160 parts of oxalic acid, extraction temperature of 65-75 ° C, single ultrasonic time of 1-2 h, the extracts are combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 3000-5000r/min, the centrifugation time is 20-45min, and the supernatant is collected;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration temperature is 55-65 ° C, and dried to obtain a black scorpion extract.

Further, in S1, the weight ratio of black sorghum fruit, hydrochloric acid, citric acid, and oxalic acid is 50-56: 120-140: 110-125: 130-145.

Further, in S2, the centrifuge speed is 4500-800 r/min, and the centrifugation time is 30-35 min.

Further, in S3, the macroporous resin is a polystyrene-divinylbenzene macroporous resin.

Further, in S4, the concentration temperature is 56-60 °C.

Further, the method for preparing the black scorpion extract comprises the following steps:

S1, weigh 50-56 parts of black sorghum fruit by weight, add 30-35 parts of water to it, mix well, seal it for 1-3 days, air-dry at room temperature, then use 120-140 parts of concentration 1.5-3wt% Ultrasonic extraction of hydrochloric acid, 110-125 parts of citric acid, 130-145 parts of oxalic acid, extraction temperature of 70-72 ° C, single ultrasonic time of 1-2 h, the extracts were combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 4500-800r/min, the centrifugation time is 30-35min, and the supernatant is collected;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration temperature is 56-60 ° C, and dried to obtain a black peony extract.

In the present invention, by extracting the black sorghum fruit and then sequentially by hydrochloric acid, citric acid and oxalic acid, not only the extract of the black scorpion extract is easy and more thorough, but also by reasonable control of the ratio of material to liquid, ultrasonic power, ultrasonic time, and ultrasonic temperature. The range and product extraction efficiency are extremely high, which not only shortens the time of extraction and separation, reduces the energy consumption in the process, and effectively reduces the production cost, and the obtained product has high purity, stable performance, less impurities and good quality, and can be applied. Raw materials for medicine and health care products with antioxidant effects. The preparation process of the black scorpion extract of the invention has the advantages of simple preparation process, low cost and high extraction efficiency, and has certain promotion and application value.

detailed description

Hereinafter, the technical solution of the present invention will be described in detail through specific embodiments.

Example 1

A method for preparing a black scorpion extract comprises the following steps:

S1, weigh 40 parts of black sorghum fruit by weight, add 45 parts of water to it, mix well, seal it for 1 day, air-dry at room temperature, then use 150 parts of concentration of 1.5wt% hydrochloric acid, 140 parts of citric acid, 120 parts Ultrasonic extraction of oxalic acid, the extraction temperature was 75 ° C, and the single ultrasonic time was 1 h, and the extracts were combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 5000r / min, the centrifugation time is 20min, the supernatant is collected;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration temperature was 65 ° C, and dried to obtain a black scorpion extract.

Example 2

A method for preparing a black scorpion extract comprises the following steps:

S1, 70 parts of black sorghum fruit were weighed by weight, 20 parts of water was added thereto, mixed uniformly, sealed for 3 days, air-dried at room temperature, and then 100 parts of concentration of 3 wt% hydrochloric acid, 100 parts of citric acid, 160 parts of oxalic acid were sequentially used. Ultrasonic extraction, extraction temperature is 65 ° C, single ultrasound time is 2 h, the extract is combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 3000r / min, the centrifugation time is 45min, the supernatant is collected;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration was 55 ° C, and dried to obtain a black scorpion extract.

Example 3

A method for preparing a black scorpion extract comprises the following steps:

S1, weigh 50 parts of black sorghum fruit by weight, smash it into 35 parts of water, mix it evenly, seal it for 1 day, air-dry at room temperature, then use 140 parts of concentration of 1.5wt% hydrochloric acid, 125 parts of citric acid, 130 parts. Ultrasonic extraction of oxalic acid, the extraction temperature was 72 ° C, the single ultrasonic time was 20 min, and the extracts were combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 4800r / min, the centrifugation time is The supernatant was collected for 30 min;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration temperature was 60 ° C, and dried to obtain a black scorpion extract.

Example 4

A method for preparing a black scorpion extract comprises the following steps:

S1, weighed 56 parts of black sorghum fruit by weight, and added 30 parts of water to mix well, sealed for 3 days, air-dried at room temperature, and then used 120 parts of concentration of 3wt% hydrochloric acid, 110 parts of citric acid, 145 parts of oxalic acid. Ultrasonic extraction, extraction temperature of 70 ° C, single ultrasound time of 1.5 h, the extract was combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 4500 r / min, the centrifugation time is 35 min, the supernatant is collected;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration temperature was 56 ° C, and dried to obtain a black scorpion extract.

Example 5

A method for preparing a black scorpion extract comprises the following steps:

S1, weighed 53 parts of black sorghum fruit by weight, pulverized into it, added 32 parts of water, mixed uniformly, sealed for 2 days, air-dried at room temperature, and then used 135 parts of concentration of 2.4 wt% hydrochloric acid, 120 parts of citric acid, 142 parts. Ultrasonic extraction of oxalic acid, the extraction temperature was 71 ° C, the single ultrasonic time was 1.5 h, and the extracts were combined;

S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 4600r / min, the centrifugation time is 32min, the supernatant is collected;

S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent;

S4, and then sent to a rotary evaporator to concentrate, the concentration temperature was 58 ° C, and dried to obtain a black scorpion extract.

The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not The invention is not limited thereto, and any equivalents or modifications of the technical solutions of the present invention and the present invention are intended to be included within the scope of the present invention.

Claims (6)

A method for preparing a black scorpion extract, comprising the steps of: S1, weigh 40-70 parts of black sorghum fruit by weight, smash it into 20-45 parts of water, mix it evenly, seal it for 1-3 days, air-dry at room temperature, then use 100-150 parts of concentration 1.5-3wt% Ultrasonic extraction of hydrochloric acid, 100-140 parts of citric acid, 120-160 parts of oxalic acid, extraction temperature of 65-75 ° C, single ultrasonic time of 1-2 h, the extracts are combined; S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 3000-5000r/min, the centrifugation time is 20-45min, and the supernatant is collected; S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent; S4, and then sent to a rotary evaporator to concentrate, the concentration temperature is 55-65 ° C, and dried to obtain a black scorpion extract. The method for preparing a black scorpion extract according to claim 1, wherein in S1, the weight ratio of sable fruit, hydrochloric acid, citric acid, and oxalic acid is 50-56: 120-140: 110-125: 130-145. . The method for producing a black scorpion extract according to claim 1, wherein in S2, the centrifuge speed is 4,500 to 4,800 r/min, and the centrifugation time is 30 to 35 min. The method for producing a black trout extract according to claim 1, wherein in S3, the macroporous resin is a polystyrene-divinylbenzene macroporous resin. The method for producing a black scorpion extract according to claim 1, wherein in S4, the concentration temperature is 56 to 60 °C. The method for preparing a black scorpion extract according to claim 1, comprising the steps of: S1, weigh 50-56 parts of black sorghum fruit by weight, add 30-35 parts of water to it, mix well, seal it for 1-3 days, air-dry at room temperature, then use 120-140 parts of concentration 1.5-3wt% Ultrasonic extraction of hydrochloric acid, 110-125 parts of citric acid, 130-145 parts of oxalic acid, extraction temperature of 70-72 ° C, single ultrasonic time of 1-2 h, the extracts were combined; S2, hot filtered, sent to a centrifuge for centrifugation, the centrifuge speed is 4500-800r/min, the centrifugation time is 30-35min, and the supernatant is collected; S3, feeding to a column packed with macroporous resin for adsorption, followed by elution with water and eluent; S4, and then sent to a rotary evaporator to concentrate, the concentration temperature is 56-60 ° C, and dried to obtain a black peony extract.