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WO2017168876A1 - Récipient d'analyse, instrument d'analyse de cible l'utilisant et procédé d'analyse de cible - Google Patents

Récipient d'analyse, instrument d'analyse de cible l'utilisant et procédé d'analyse de cible Download PDF

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Publication number
WO2017168876A1
WO2017168876A1 PCT/JP2016/087698 JP2016087698W WO2017168876A1 WO 2017168876 A1 WO2017168876 A1 WO 2017168876A1 JP 2016087698 W JP2016087698 W JP 2016087698W WO 2017168876 A1 WO2017168876 A1 WO 2017168876A1
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WO
WIPO (PCT)
Prior art keywords
chamber
reagent
binding substance
target
cylinder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2016/087698
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English (en)
Japanese (ja)
Inventor
克紀 堀井
嘉仁 吉田
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NEC Solution Innovators Ltd
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NEC Solution Innovators Ltd
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Filing date
Publication date
Application filed by NEC Solution Innovators Ltd filed Critical NEC Solution Innovators Ltd
Priority to JP2018508392A priority Critical patent/JP6525301B2/ja
Publication of WO2017168876A1 publication Critical patent/WO2017168876A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/26—Inoculator or sampler
    • C12M1/28—Inoculator or sampler being part of container
    • C12M1/30—Sampler being a swab
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q

Definitions

  • the present invention relates to an analysis container, a target analysis tool using the same, and a target analysis method.
  • Patent Document 1 In target analysis, generally, a binding substance having binding properties to the target is used, and a conjugate of the target and the binding substance is formed, and this is detected directly or indirectly, The presence / absence or amount of the target can be analyzed (Patent Document 1, Patent Document 2).
  • the binding substance bound to the target and the target not bound to the target are placed in a bottomed cylindrical analysis container.
  • An analytical container in which a partition wall capable of separating the binding substance of the binding was arranged was considered.
  • the analysis container in the chamber on the opening side of the partition wall, a combined body of the target and the binding substance is formed, and then the mixture of the target and the binding substance is passed through the partition wall to be on the bottom side.
  • the analysis container has a problem that the mixture of the target and the binding substance does not substantially move from the opening-side chamber to the bottom-side chamber via the partition wall.
  • the target and the binding substance are formed in the chamber on the opening side before the formation body of the target and the binding substance is formed.
  • the mixture with the binding substance may pass through the partition and be introduced into the bottom chamber. For this reason, there is a problem that sufficient analysis accuracy may not be obtained.
  • an object of the present invention is to provide an analysis container capable of controlling the flow of liquid in the analysis container, a target analysis tool and a target analysis method using the same.
  • the analysis container of the present invention includes a first chamber, a second chamber, and a third chamber, The first chamber, the second chamber, and the third chamber are sequentially arranged in this order, A first partition between the first chamber and the second chamber; A second partition wall between the second chamber and the third chamber; The first chamber can be inserted with a sample holding tool from the outside to the inside, The first partition is a partition that is destroyed by contacting the tip of the sample holding tool inserted into the first chamber, The second partition is a porous partition,
  • the third chamber includes one or more through holes, In the through hole, a closing member that can be opened and closed is arranged in a state of covering the through hole, By opening the through hole from the closing member, liquid can be passed from the second chamber to the third chamber.
  • the target analysis tool of the present invention includes the analysis container of the present invention, a first reagent, and a second reagent,
  • the first chamber contains the first reagent
  • the second chamber contains the second reagent
  • the third chamber is a detection unit for detecting a labeling substance in the first reagent or the second reagent
  • the second partition wall is a porous partition wall through which the binding substance immobilized on the carrier cannot pass and the binding substance bound with the labeling substance can pass through.
  • the first reagent and the second reagent are any combination of the following (1) to (3) and (4).
  • the first reagent is an immobilized second binding substance in which a second binding substance that binds to a first binding substance that binds to a target is immobilized on a carrier, and the second reagent is the first binding substance. It is a labeled first binding substance in which a labeling substance is bound to a substance.
  • the first reagent is a labeled first binding substance in which a labeling substance is bound to a first binding substance that binds to a target, and the second reagent is a second binding substance that binds to the first binding substance. Is an immobilized second binding substance immobilized on a carrier.
  • the first reagent is a labeled second binding substance in which a labeling substance is bound to a second binding substance that binds to a first binding substance that binds to a target, and the second reagent is the first binding substance. Is an immobilized first binding substance immobilized on a carrier.
  • the first reagent is an immobilized first binding substance in which a first binding substance that binds to a target is fixed to a carrier, and the second reagent binds to the first binding substance. This is a labeled second binding substance in which a labeling substance is bound.
  • the target analysis method of the present invention uses the above-described target analysis tool of the present invention and performs any of the following analysis methods (A) to (C) and (D). It is characterized by doing.
  • the sample, the first reagent, and the second reagent are brought into contact with each other, and the target in the sample and the labeled first binding substance that is the second reagent are bound to each other. And binding the labeled first binding substance unbound to the target to the immobilized second binding substance that is the first reagent, Introducing the first combined body into the third chamber through a second partition between the second chamber and the third chamber; and An analysis method comprising a step of detecting the labeled first binding substance in the first conjugate in the third chamber.
  • Binding to the immobilized second binding substance Introducing the first combined body into the third chamber through a second partition between the second chamber and the third chamber; and An analysis method comprising a step of detecting the labeled first binding substance in the first conjugate in the third chamber.
  • C Analytical method The target reagent which said 1st reagent and said 2nd reagent are the combination of said (3), Inserting a sample holding tool holding a sample into the first chamber of the target analysis tool; In the second chamber, the second reagent is brought into contact with the mixture of the sample and the first reagent to bind the target in the sample and the immobilized first binding substance as the second reagent.
  • An analysis method comprising a step of detecting the labeling substance in the labeled second binding substance in the third chamber.
  • the first reagent and the second reagent are a combination of (4), Inserting a sample holding tool holding a sample into the first chamber of the target analysis tool; Contacting the first reagent and the sample in the first chamber to bind the target in the sample and the immobilized first binding substance as the first reagent; The sample holding tool in the first chamber is brought into contact with a first partition between the first chamber and the second chamber, the partition is broken, and the sample in the first chamber is placed
  • the liquid flow in the analysis container can be controlled.
  • FIG. 1 is a schematic cross-sectional view illustrating a configuration of an analysis container according to Embodiment 1 and a schematic diagram illustrating an example of liquid movement in the analysis container.
  • FIG. 2 is a schematic cross-sectional view illustrating a configuration of an analysis container according to the second embodiment and a schematic diagram illustrating an example of liquid movement in the analysis container.
  • FIG. 3 is a schematic cross-sectional view and a schematic exploded view showing the configuration of the analysis container of the third embodiment.
  • FIG. 4 is a schematic diagram illustrating an example of the target analysis tool of Embodiment 4A.
  • FIG. 5 is a schematic diagram illustrating an example of an analysis method using the target analysis tool of Embodiment 4A.
  • FIG. 6 is a schematic diagram illustrating an example of the target analysis tool of Embodiment 4B.
  • FIG. 7 is a schematic diagram illustrating an example of an analysis method using the target analysis tool of Embodiment 4B.
  • FIG. 8 is a schematic diagram illustrating an example of the target analysis tool of Embodiment 4C.
  • FIG. 9 is a schematic diagram illustrating an example of an analysis method using the target analysis tool of Embodiment 4C.
  • FIG. 10 is a schematic diagram illustrating an example of a target analysis tool according to Embodiment 4D.
  • FIG. 11 is a schematic diagram illustrating an example of an analysis method using the target analysis tool of Embodiment 4D.
  • the closing member is a peelable seal member
  • the seal member On the outer surface of the third chamber, the seal member is disposed in a state of covering the through hole, By peeling the sealing member from the through hole, liquid can be passed from the second chamber to the third chamber.
  • the closing member is a removable rod-shaped member
  • the rod-shaped member is disposed on the outer surface of the third chamber so as to cover the through-hole, By removing the rod-shaped member from the through hole, liquid can be passed from the second chamber to the third chamber.
  • the first chamber has a pre-partition wall that is destroyed by bringing the tip of the sample holding tool into contact with the side opposite to the second chamber.
  • the analysis container of the present invention has, for example, a first cylinder, a second cylinder, and a third cylinder,
  • the second cylinder can be accommodated in the first cylinder;
  • the third cylinder can be disposed at an end of the first cylinder;
  • the second cylinder has the first chamber;
  • the third cylinder has the third chamber;
  • the first cylinder has a positioning member that determines an accommodation position of the second cylinder in the first cylinder,
  • the second cylinder has the front partition and the first partition;
  • the third cylinder has the second partition wall and a connection member for connecting to the first cylinder,
  • the second cylinder is disposed at a predetermined position in the first cylinder by the positioning member;
  • the third cylinder is disposed at an end of the first cylinder via the connection member.
  • the analysis container of the present invention has, for example, an outer cylinder and an inner cylinder,
  • the inner cylinder can be accommodated in the outer cylinder;
  • the inner cylinder has the first chamber and the second chamber;
  • a space is provided between the bottom of the outer cylinder and the bottom of the inner cylinder.
  • the first binding substance is an aptamer
  • the second binding substance is a nucleic acid molecule complementary to the aptamer.
  • the labeling substance is at least one substance selected from the group consisting of an enzyme, a nucleic acid, a fluorescent substance, a dye substance, a luminescent substance, a radioactive substance, and an electron donor.
  • the enzyme is preferably luciferase.
  • the third chamber contains a substrate for the enzyme.
  • the carrier is a bead.
  • the sample holding tool includes a rod-shaped gripping part and a sample holding part,
  • the holding part is provided at the tip of the grip part.
  • the analysis method of the present invention is, for example, the analysis method of (B) or (C),
  • the sample holding tool in the first chamber is brought into contact with a first partition between the first chamber and the second chamber, the partition is broken, and the sample in the first chamber is placed in the second chamber. And introducing a mixture of the first reagent and the first reagent.
  • the “upward direction” means, for example, a direction perpendicular to the surface direction of the bottom surface of the analysis container of the present invention, and means the second chamber and the first chamber direction from the third chamber,
  • the “down (bottom) direction” means a direction opposite to the upward direction.
  • the analysis container of the present invention includes the first chamber, the second chamber, and the third chamber, and the first chamber, the second chamber, and the third chamber are sequentially arranged in this order. And having a first partition between the first chamber and the second chamber, and having a second partition between the second chamber and the third chamber, A sample holding tool can be inserted from the outside into the inside, and the first partition is a partition that is broken by bringing the tip of the sample holding tool inserted into the first chamber into contact with the second partition.
  • the partition wall is a porous partition wall
  • the third chamber includes one or more through holes
  • an openable and closable closing member is disposed in the through hole so as to cover the through hole.
  • the third chamber includes one or more through holes, and an opening / closing closing member is disposed in the through hole so as to cover the through hole. It is a feature that the liquid can be passed from the second chamber to the third chamber by opening from the closing member, and other configurations and conditions are not particularly limited.
  • the blocking member can be said to be a member capable of controlling the opening and closing of the through hole, for example.
  • the liquid passes through the second partition wall between the second chamber and the third chamber, and enters the third chamber. Try to move.
  • the closing member is disposed in a state of covering the through hole, for example, the air in the third chamber cannot move from the third chamber.
  • the liquid introduced into the third chamber by being replaced with the air does not substantially move from the second chamber to the third chamber, for example.
  • the blocking member is removed and the through hole is opened from the blocking member, for example, the air in the third chamber can be moved out of the analysis container via the through hole. That is, the third chamber can be vented, for example.
  • the liquid can move from the second chamber to the third chamber, and moves from the second chamber to the third chamber. Therefore, according to the analytical container of the present invention, liquid flow from the second chamber to the third chamber can be easily controlled by controlling the opening and closing of the through hole by the closing member.
  • FIG. 1A is a schematic cross-sectional view of the analysis container 1 of this embodiment.
  • FIGS. 1B and 1C show the second chamber 12 when the through-hole 14 is opened and closed in the analysis container 1. It is a schematic diagram showing the movement of the introduced liquid.
  • the analysis container 1 of the present embodiment has a first chamber 11, a second chamber 12, and a third chamber 13, and the third chamber 13 has a through hole 14.
  • the analysis container 1 has a first partition 111 between the first chamber 11 and the second chamber 12, and a porous second partition between the second chamber 12 and the third chamber 13. 121.
  • a sealing member 151 which is the closing member, is disposed so as to cover the through hole 14.
  • the seal member 151 can be peeled off from the outer surface of the third chamber 13.
  • the analysis container 1 of the present embodiment when the analysis container 1 of the present embodiment is arranged in a state where the seal member 151 covers the through hole 14, the air in the third chamber 13 flows from the third chamber 13. I can't move. For this reason, for example, the liquid introduced into the second chamber 12 does not substantially move from the second chamber 12 to the third chamber 13. 1C, when the seal member 151 is removed and the through hole 14 is released from the seal member 151, for example, the air in the third chamber 13 is analyzed via the through hole 14. It becomes possible to move out of the container 1. Therefore, for example, the liquid in the second chamber 12 can move from the second chamber 12 to the third chamber 13, and moves from the second chamber 12 to the third chamber 13. Therefore, according to the analysis container 1 of the present embodiment, the opening and closing of the through hole 14 can be controlled by peeling off the seal member 151, and the liquid flow from the second chamber 12 to the third chamber 13 can be easily controlled.
  • the shape of the analysis container 1 of the present embodiment is not particularly limited and can be any shape.
  • the material for forming the analytical container 1 of the present embodiment is not particularly limited and may be any material. Specific examples thereof include plastics such as polyethylene, polystyrene, polypropylene, acrylonitrile-butadiene-styrene copolymer synthetic resin, and the like.
  • the sizes of the first chamber 11, the second chamber 12, and the third chamber 13 are not particularly limited, and can be appropriately designed according to, for example, the volume of the sample to be analyzed.
  • the third chamber 13 has one through hole 14, but the present invention is not limited to this, and the third chamber 13 has two or more through holes 14. Also good.
  • the through hole 14 is formed in the upper part of the side surface of the third chamber 13, but the present invention is not limited to this, for example, a liquid is introduced into the third chamber 13. In this case, the liquid may be formed at any position where the liquid does not substantially flow out of the analysis container 1.
  • the shape of the through hole 14 is not particularly limited and can be an arbitrary shape.
  • the size of the through hole 14 is not particularly limited, and may be any size as long as air inside and outside the analysis container 1 can be vented through the through hole 14.
  • the diameter of the through hole 14 in the cross-sectional direction from the inner surface to the outer surface direction of the analytical container 1 is, for example, 0.01 to 2.0 mm, preferably 0.5 to 1.5 mm.
  • the first partition 111 between the first chamber 11 and the second chamber 12 is a partition that is destroyed by bringing the tip of the sample holding tool into contact therewith.
  • the first partition 111 is, for example, the bottom of the first chamber 11 and the top of the second chamber 12.
  • the first partition 111 may be broken by bringing the tip of the sample holding tool into contact with the first partition 111, and the material, characteristics, and the like are not particularly limited.
  • metal thin films such as aluminum foil, paper, a synthetic fiber, etc. can be used, for example.
  • the sample holding tool will be described later.
  • the second partition 121 between the second chamber 12 and the third chamber 13 is a porous partition as described above.
  • the porous partition is not particularly limited, and examples thereof include a porous film.
  • the porous membrane include cellulose membranes, cellulose derivative membranes such as cellulose acetate and nitrocellulose, filters such as glass filters, filter papers, and the like.
  • the pore diameter in the porous partition wall is not particularly limited and can be appropriately set according to the reagent or the like placed in the analysis container 1.
  • the seal member 151 is disposed on the outer surface of the third chamber 13 so as to cover the through hole 14.
  • the present invention is not limited to this, and for example, the seal member 151 may block the inside of the through hole 14.
  • the seal member 151 is not particularly limited, and may be any material, for example, an adhesive tape, an adhesive tape, or the like.
  • positioning method of the sealing member 151 should just be peelable, for example, methods, such as adhesion
  • the seal member 151 may be capable of closing the through hole 14 again after peeling, for example.
  • the analysis container 1 of the present embodiment may have, for example, an outer cylinder and an inner cylinder.
  • the inner cylinder can be accommodated in the outer cylinder, the inner cylinder has the first chamber and the second chamber, and when the inner cylinder is accommodated in the outer cylinder, There is a space between the bottom of the outer cylinder and the bottom of the inner cylinder.
  • the outer cylinder and the inner cylinder are provided, the outer cylinder is removed by removing the inner cylinder after introducing the mixture of the target and the binding substance from the second chamber of the inner cylinder to the outer cylinder. It can be used as the third chamber.
  • the analysis container 1 of the present embodiment may further include a pretreatment chamber, for example.
  • the first chamber may be arranged continuously to the pretreatment chamber on the side opposite to the second chamber. That is, in the analysis container 1 of the present embodiment, the pretreatment chamber, the first chamber, the second chamber, and the third chamber may be arranged in this order.
  • the sample processing tool can be inserted into the pretreatment chamber from the outside to the inside, for example.
  • the pretreatment chamber includes, for example, an extract that extracts components inside the sample from the sample.
  • the extract is not particularly limited, and can be appropriately selected depending on the type of sample to be analyzed, the type of component to be analyzed, and the like.
  • the analysis container 1 of the present embodiment has, for example, a partition between the pretreatment chamber and the first chamber, and the partition contacts the tip of the sample holding tool inserted in the pretreatment chamber. It is a partition which is destroyed by making it.
  • the partition wall to be destroyed is the same as described above, for example, an aluminum foil or the like.
  • the pretreatment chamber has a cover of the opening of the pretreatment chamber on the opposite side of the partition wall from the first chamber, and the cover is provided from the outside.
  • the sample holding tool can be inserted inside.
  • the cover is preferably a partition that is broken by bringing the tip of the sample holding tool into contact with the cover, and examples include the same partition as described above.
  • FIG. 2A is a schematic cross-sectional view of the analysis container 2 of the present embodiment.
  • FIGS. 2B and 2C show the second chamber 12 when the through-hole 14 is opened and closed in the analysis container 2. It is a schematic diagram showing the movement of the introduced liquid.
  • the analysis container 2 of this embodiment has a rod-shaped member 152 as the closing member instead of the seal member 151, and the rod-shaped member 152 is formed on the outer surface of the third chamber 13.
  • positioned in the state which covers the through-hole 14 it has the structure similar to the container 1 for analysis of Embodiment 1, and can use the description.
  • the opening and closing of the through hole 14 can be controlled by removing the rod-shaped member 152, and the liquid flow from the second chamber 12 to the third chamber 13 can be easily controlled.
  • the rod-shaped member 152 is arranged on the outer surface of the third chamber 13 so as to cover the through hole 14.
  • the present invention is not limited to this, and for example, the rod-shaped member 152 may block the inside of the through hole 14.
  • the rod-shaped member 152 is not particularly limited, and can be any material, for example, a rod-shaped plastic.
  • the rod-shaped member 152 may be made of the same material as that of the analysis container 2 or may be made of a different material.
  • the arrangement method of the rod-shaped member 152 may be a known arrangement method capable of removing the rod-shaped member 152, and examples thereof include a method such as heat fusion.
  • the rod-shaped member 152 may be able to close the through-hole 14 again after removal.
  • FIG. 3A is a schematic cross-sectional view of the analysis container 3 of the present embodiment
  • FIG. 3B is a schematic exploded view of the analysis container 3.
  • the analysis container 3 of the present embodiment has a first cylinder 31, a second cylinder 32, and a third cylinder 33, and the first cylinder 31 is the first cylinder 31.
  • the second cylinder 32 has a first chamber 11, a first partition wall 111 and a front partition wall 112, and the third cylinder 33 has a third chamber 13,
  • the connecting member 17 is formed as a cylinder in contact with the inner surface of the second partition wall 121, the through hole 14, and the first cylinder 31.
  • the second cylinder 32 is accommodated in contact with the inner surface of the first cylinder 31, and the third cylinder 33 has the connection member 17 in contact with the inner surface of the first cylinder 31 on the lower end side of the first cylinder 31. Thus, it is arranged at the end of the first cylinder 31.
  • connection member 17 of the third cylinder 33 can also be inserted into the first cylinder 31 from below the first cylinder 31.
  • the analysis container 3 of the present embodiment has the same configuration as the analysis container 1 of the first embodiment, and the description thereof can be used. According to the analysis container 3 of the present embodiment, the analysis container can be easily assembled by combining three cylinders. For this reason, according to the container 3 for analysis of this embodiment, it can manufacture simply.
  • the first cylinder 31 includes the positioning member 16, but the positioning member 16 may have any configuration and may or may not be provided.
  • the positioning member 16 is formed as a convex portion in the inner direction of the first cylinder 31, but the present invention is not limited to this, and the positioning member 16 includes the second cylinder 32.
  • Known fixing means that can fix the position can be used.
  • the position of the positioning member 16 in the first cylinder 31 is not particularly limited. When the second cylinder 32 is accommodated in the first cylinder 31 and the third cylinder 33 is disposed at the end of the first cylinder 31. Any position that allows the second cylinder 32 to be disposed so as to have a space between the bottom of the second cylinder 32 and the upper portion of the third cylinder 33 may be used.
  • the second cylinder 32 includes the front partition 112, but the front partition 112 may have any configuration and may or may not be provided.
  • the front partition 112 is a partition that is destroyed by contacting the tip of the sample holding tool. It can be said that the front partition 112 is, for example, the upper part of the first chamber 11.
  • the front partition 112 may be destroyed by bringing the tip of the sample holding tool into contact with the front partition 112, and the material, characteristics, and the like are not particularly limited.
  • a metal thin film such as aluminum foil, paper, synthetic fiber, or the like can be used.
  • the front partition 112 may be made of the same material as the first partition 111 or may be made of a different material.
  • the third cylinder 33 includes the connection member 17, but the connection member 17 may have any configuration and may or may not be provided.
  • the connection member 17 is formed as a cylinder in contact with the inner surface of the first cylinder 31, but the present invention is not limited to this, and the connection member 17 connects two members.
  • Known connection means can be used.
  • the position of the connection member 17 in the third cylinder 33 is not particularly limited, and can be appropriately set according to the connection means.
  • the first reagent is an immobilized second binding substance in which a second binding substance that binds to a first binding substance that binds to a target is immobilized on a carrier, and the second reagent is the first binding substance. It is a labeled first binding substance in which a labeling substance is bound to a substance.
  • the first reagent is a labeled first binding substance in which a labeling substance is bound to a first binding substance that binds to a target, and the second reagent is a second binding substance that binds to the first binding substance. Is an immobilized second binding substance immobilized on a carrier.
  • the target analysis method of the present invention is characterized in that, as described above, the target analysis tool of the present invention is used and any one of the following analysis methods (A) to (C) and (D) is performed. And (A) Analysis method Using the target analysis tool, wherein the first reagent and the second reagent are a combination of (1), After a sample holding tool holding a sample is inserted into the first chamber of the target analysis tool, the sample holding tool is brought into contact with the first partition between the first chamber and the second chamber, and the sample is placed in the second chamber.
  • An analysis method comprising a step of detecting the labeling substance in the labeled second binding substance in the third chamber.
  • the first reagent and the second reagent are a combination of (4), Inserting a sample holding tool holding a sample into the first chamber of the target analysis tool; Contacting the first reagent and the sample in the first chamber to bind the target in the sample and the immobilized first binding substance as the first reagent; The sample holding tool in the first chamber is brought into contact with a first partition between the first chamber and the second chamber, the partition is broken, and the sample in the first chamber is placed
  • the analysis may be, for example, a qualitative analysis that determines the presence or absence of the target, or a quantitative analysis that determines the amount of the target.
  • the first binding substance that binds to the target used in the present invention may be bound to the target, for example, and the type thereof is not particularly limited.
  • Specific examples of the first binding substance include aptamers and antibodies.
  • the present embodiment is an embodiment of the analysis method of (A) in which the first reagent and the second reagent use the analysis tool of the combination of (1) and the analysis tool of the combination of (1). .
  • the analysis tool of Embodiment 4A includes the analysis container of the present invention, a first reagent, and a second reagent
  • the first chamber includes, as the first reagent, an immobilized second binding substance in which a second binding substance that binds to a first binding substance that binds to a target is immobilized on a carrier
  • the second chamber includes, as the second reagent, a labeled first binding substance in which a labeling substance is bound to the first binding substance
  • the third chamber is a detection unit for detecting the labeled first binding substance
  • the second partition wall is a porous partition wall through which the immobilized second binding substance cannot pass, but through which the first combined body in which the target in the sample and the labeled first binding substance as the second reagent are bound can pass through. It is.
  • the analysis method of Embodiment 4A uses the target analysis tool of Embodiment 4A, After a sample holding tool holding a sample is inserted into the first chamber of the target analysis tool, the sample holding tool is brought into contact with the first partition between the first chamber and the second chamber, and the sample is placed in the second chamber. And introducing the first reagent, In the second chamber, the sample, the first reagent, and the second reagent are brought into contact with each other, and the target in the sample and the labeled first binding substance that is the second reagent are bound to each other.
  • the method includes detecting the labeled first binding substance in the first conjugate.
  • the target in the sample and the immobilized second binding substance that is the first reagent are mixed.
  • the labeling that is the target in the sample and the second reagent is performed in the second chamber.
  • the first binding substance binds to the unbound labeled first binding substance and the immobilized second binding substance as the first reagent.
  • the second partition between the second chamber and the third chamber cannot pass through the immobilized second binding substance, and can pass through the first conjugate containing the labeled first binding substance. Since the barrier rib is an insulating partition, the immobilized second binding substance remains in the second chamber without passing through the partition.
  • the labeled first binding substance bound to the immobilized second binding substance remains in the second chamber without moving to the third chamber.
  • the labeled first binding substance released unbound to the immobilized second binding substance that is, the labeled first binding substance forming the first conjugate passes through the partition wall and passes through the partition wall. Introduced into the third chamber. Since the labeled first binding substance in the analysis tool of the present embodiment can be, for example, a known amount, the amount of the labeled first binding substance unbound to the immobilized second binding substance is This indirectly corresponds to the target amount in the sample. Therefore, the presence or amount of the target in the sample can be indirectly analyzed by detecting the unbound labeled first binding substance introduced into the third chamber.
  • the analysis container of Embodiment 1 is used as the analysis container, and the aptamer is used as the first binding substance
  • the analysis container of Embodiment 1 is used as the analysis container, and the aptamer is used as the first binding substance
  • the second binding substance in the immobilized second binding substance only needs to be able to bind to the aptamer.
  • a nucleic acid molecule complementary to the aptamer hereinafter referred to as “complementary nucleic acid molecule” or “complementary strand”).
  • the term “complementary to the aptamer” is not limited to 100% complementarity, for example, as long as the aptamer or the partial sequence thereof is capable of hybridizing.
  • the complementarity is, for example, 95% or more, 96% or more, 97% or more, 98% or more, 99% or more, 100%.
  • Examples of the carrier in the immobilized second binding substance include beads.
  • the material of the beads is not particularly limited, and examples thereof include polymers such as agarose, sepharose, and cellulose.
  • Examples of the beads include magnetic beads.
  • the magnetic bead may be, for example, a bead made of a magnetic material, a bead containing the magnetic material, or a bead whose surface is coated with the magnetic material.
  • Examples of the magnetic material include a magnetizable substance, and specific examples include ⁇ Fe 2 O 3 , Fe 3 O 4, and the like.
  • the shape of the beads is not particularly limited, and examples thereof include a spherical shape such as a true spherical shape.
  • the catalyst nucleic acid molecule is not particularly limited, and examples thereof include DNAzyme and RNAzyme.
  • the catalytic function of the catalytic nucleic acid molecule in the labeled first binding substance preferably exhibits a catalytic function regardless of whether the target is bound to the labeled first binding substance, for example.
  • the binding form of the binding substance and the catalytic nucleic acid molecule is not particularly limited, and is, for example, a phosphodiester bond.
  • the binding substance and the catalytic nucleic acid molecule may be bound directly or indirectly via a linker, for example.
  • the linker is, for example, a nucleic acid molecule composed of at least one of DNA and RNA.
  • the sample is not particularly limited, and examples thereof include food-derived samples.
  • the food-derived sample include foods, food raw materials, food additives, deposits in food processing plants or kitchens, washing liquids after washing, and the like.
  • the form of the sample is not particularly limited, and may be, for example, a liquid sample or a solid sample.
  • a mixed solution, an extract, a dissolved solution, or the like may be prepared using a solvent and used as the sample.
  • the solvent is not particularly limited, and examples thereof include water, physiological saline, and buffer solution.
  • the sample may be, for example, a sample including the target, a sample not including the target, or a sample unknown whether the target is included.
  • the second partition wall between the second chamber and the third chamber is a porous partition wall through which the immobilized second binding substance cannot pass and the first combined body can pass.
  • the hole diameter of the second partition wall can be appropriately set according to the size of the immobilized second binding substance and the first combined body, for example.
  • the hole diameter in the second partition wall is, for example, 0.2-100 ⁇ m, 0.2-50 ⁇ m, 0.5-10 ⁇ m.
  • the aptamer that is the first binding substance that binds to the target and the complementary nucleic acid molecule that is the second binding substance that binds to the first binding substance are both nucleic acids.
  • the third chamber preferably further contains, for example, a substrate for its catalytic function.
  • a substrate for its catalytic function examples include a combination of ATP and luciferin, a luminol reaction solution, and the like.
  • FIG. 4 is a diagram showing an outline of the analysis tool of the embodiment 4A.
  • the analysis tool 4 has a first chamber 11, a second chamber 12, and a third chamber 13, and an immobilized complementary strand 19 in which a complementary strand 191 for the aptamer 181 is immobilized on a bead 192 is contained in the first chamber 11.
  • a labeled aptamer 18 obtained by adding an enzyme 182 to the aptamer 181 is disposed in the second chamber 12.
  • a first partition 111 is provided between the first chamber 11 and the second chamber 12, and a porous second partition 121 is provided between the second chamber 12 and the third chamber 13.
  • FIG. 5 is a schematic view showing how to use the analytical tool 4.
  • the first partition 111 is broken by the sample holding tool 20, and the contents in the first chamber 11 are introduced into the second chamber 12. Then, the target 21 in the contents is bound to the labeled aptamer 18, and among the labeled aptamers 18, the labeled aptamer 18 that is not bound to the target 21 is bound to the immobilized complementary strand 19. Further, a sealing member 151 is disposed on the outer surface of the third chamber 13 so as to cover the through hole 14 during the coupling process of the both.
  • the treatment conditions for the combination of the two are not particularly limited, and the temperature is, for example, 4 to 37 ° C., and the time is, for example, 10 seconds to 30 minutes.
  • the bonding between the two is preferably performed, for example, in the liquid solvent.
  • the liquid can be passed from the second chamber 12 to the third chamber 13. Since the second partition 121 is a porous partition that does not pass through the immobilized complementary strand 19 and passes through the first conjugate of the labeled aptamer 18 and the target 21, the immobilized complementary strand of the labeled aptamer 18. Only the unbonded material 19 passes through the second partition 121 and is introduced into the third chamber 13. Then, in the third chamber 13, the target in the sample can be indirectly analyzed by measuring the catalytic function of the enzyme 182 for the labeled aptamer 18 that is not bound to the immobilized complementary strand 19. The measurement of the catalytic function of the enzyme 182 can be appropriately determined according to the type of the enzyme 182.
  • the analysis method of Embodiment 4B uses the target analysis tool of Embodiment 4B, Inserting a sample holding tool holding a sample into the first chamber of the target analysis tool; Contacting the first reagent with the sample in the first chamber to form a first conjugate in which the target in the sample is bound to the labeled first binding substance as the first reagent; In the second chamber, the second reagent is brought into contact with the mixture of the sample and the first reagent, and the labeled first binding substance unbound to the target in the mixture is used as the second reagent. Binding to the immobilized second binding substance, Introducing the first combined body into the third chamber through a second partition between the second chamber and the third chamber; and In the third chamber, the method includes detecting the labeled first binding substance in the first conjugate.
  • the labeled first binding substance bound to the immobilized second binding substance remains in the second chamber without moving to the third chamber.
  • the labeled first binding substance released unbound to the immobilized second binding substance that is, the labeled first binding substance forming the first conjugate passes through the partition wall and passes through the partition wall. Introduced into the third chamber. Since the labeled first binding substance in the analysis tool of the present embodiment can be, for example, a known amount, the amount of the labeled first binding substance unbound to the immobilized second binding substance is This indirectly corresponds to the target amount in the sample. Therefore, the presence or amount of the target in the sample can be indirectly analyzed by detecting the unbound labeled first binding substance introduced into the third chamber.
  • the analysis container of the first embodiment is used as the analysis container, and the aptamer is used as the first binding substance.
  • the form to be described will be described by way of example.
  • the embodiment 4B for example, the description of the embodiments 1 to 3 and 4A can be cited.
  • This embodiment is a form in which the aptamer is used as the first binding substance in the labeled first binding substance, and the aptamer is the same as that in Embodiment 4A, for example.
  • the second binding substance in the immobilized second binding substance only needs to be able to bind to the aptamer, and examples thereof include a nucleic acid molecule complementary to the aptamer.
  • the nucleic acid molecule complementary to the aptamer is, for example, the same as in Embodiment 4A.
  • the labeling substance in the labeled first binding substance is preferably, for example, a catalytic nucleic acid molecule or an enzyme exhibiting a catalytic function, and the labeling substance, the catalytic nucleic acid molecule, and the enzyme are the same as in, for example, Embodiment 4A .
  • the carrier in the immobilized second binding substance may be, for example, a bead or the inner wall of the second chamber.
  • the beads are the same as in Embodiment 4A, for example.
  • the amount of the second binding substance immobilized on the carrier (bead) is not particularly limited, and for example, 0.1 fmol to 100 pmol, 1 fmol to 10 pmol per 1 mm 2 of the surface area of the bead, 10 fmol to 1 pmol.
  • the amount of the second binding substance immobilized on the carrier is not particularly limited, for example, per 1 mm 2 area of the inner wall of the second chamber, 0.1 fmol to 100 pmol, 1 fmol to 10 pmol, 10 fmol to 1 pmol.
  • aptamers can be used as the first binding substance and complementary nucleic acid molecules can be used as the second binding substance, for example, it is heat-stable and is easier to store.
  • an enzyme such as luciferase, alkaline phosphatase or peroxidase can be used as the labeling substance, for example, the target can be analyzed with high sensitivity.
  • the first chamber may further include, for example, an extract for extracting the components inside the sample.
  • an extract for extracting the components inside the sample.
  • the extract is the same as in Embodiment 4A.
  • the second chamber may further include an adsorption carrier that adsorbs at least one of protein and lipid as the second reagent.
  • the adsorption carrier is the same as that in the embodiment 4A, for example.
  • the third chamber preferably further contains a substrate for the catalytic function of the catalytic nucleic acid molecule or the enzyme, for example.
  • the substrate is the same as that in Embodiment 4A, for example.
  • FIG. 6 is a diagram showing an outline of the analysis tool of the embodiment 4B.
  • the analysis tool 5 a labeled aptamer 18 in which an enzyme 182 is added to an aptamer 181 is arranged in the first chamber 11, and a complementary strand 191 for the aptamer 181 is immobilized in the beads 192 in the second chamber 12.
  • a complementary strand 19 is arranged.
  • the analysis tool 5 of this embodiment has the same configuration as the analysis tool 4 of Embodiment 4A, and the description thereof can be used.
  • FIG. 7 is a schematic view showing how to use the analytical tool 5.
  • the sample holding tool 20 in which the sample is held in the holding unit 202 is inserted into the first chamber 11 of the analysis tool 5, and the target 21 in the sample is bound to the aptamer 181 of the labeled aptamer 18 in the first chamber 11.
  • the treatment conditions for the combination of the two are not particularly limited, and the temperature is, for example, 4 to 37 ° C., and the time is, for example, 10 seconds to 30 minutes.
  • the combination of the two is preferably performed in, for example, a liquid solvent, and examples of the liquid solvent include aqueous solvents such as water, buffer solution, physiological saline, and a mixed solution thereof.
  • the liquid can be passed from the second chamber 12 to the third chamber 13. Since the second partition 121 is a porous partition that does not pass through the immobilized complementary strand 19 and passes through the first conjugate of the labeled aptamer 18 and the target 21, the immobilized complementary strand of the labeled aptamer 18. Only the unbonded material 19 passes through the second partition 121 and is introduced into the third chamber 13. Then, in the third chamber 13, the target in the sample can be indirectly analyzed by measuring the catalytic function of the enzyme 182 for the labeled aptamer 18 that is not bound to the immobilized complementary strand 19. The measurement of the catalytic function of the enzyme 182 can be appropriately determined according to the type of the enzyme 182.
  • This embodiment is an embodiment of the analysis method of (C) using the analysis tool of the combination of (3) and the analysis tool of the combination of (3) as the first reagent and the second reagent. .
  • the analysis tool of Embodiment 4C includes the analysis container of the present invention, a first reagent, and a second reagent
  • the first chamber includes, as the first reagent, a labeled second binding substance in which a labeling substance is bound to a second binding substance that binds to a first binding substance that binds to a target
  • the second chamber includes, as the second reagent, an immobilized first binding substance in which the first binding substance is immobilized on a carrier
  • the third chamber is a detection unit for detecting the labeled second binding substance
  • the second partition wall is a porous partition wall through which the immobilized first binding substance cannot pass and the labeled second binding substance can pass through.
  • the analysis method of Embodiment 4C uses the target analysis tool of Embodiment 4C, Inserting a sample holding tool holding a sample into the first chamber of the target analysis tool; In the second chamber, the second reagent is brought into contact with the mixture of the sample and the first reagent to bind the target in the sample and the immobilized first binding substance as the second reagent. And binding the immobilized first binding substance that is unbound to the target and the labeled second binding substance that is the first reagent, Introducing unbound labeled second binding substance through the second partition between the second chamber and the third chamber into the third chamber; and The step of detecting the labeling substance in the labeled second binding substance in the third chamber is included.
  • the first chamber for example, a target in a sample and the labeled second binding substance as the first reagent are mixed. Then, when the mixture of the sample and the first reagent in the first chamber is introduced into the second chamber, the immobilization that is the target in the sample and the second reagent in the second chamber. The immobilized first binding substance that binds to the first binding substance and is not bound to the target binds to the labeled second binding substance that is the first reagent. And since the second partition between the second chamber and the third chamber is the porous partition wall through which the immobilized first binding substance cannot pass and the labeled second binding substance can pass through, The immobilized first binding substance remains in the second chamber without passing through the partition wall.
  • the analysis container of the first embodiment is used as the analysis container, and the aptamer is used as the first binding substance.
  • the form to be described will be described by way of example.
  • the embodiment 4C for example, the description of the embodiments 1 to 3, 4A, 4B and the like can be cited.
  • the second binding substance in the labeled second binding substance only needs to be able to bind to the aptamer, and examples thereof include a nucleic acid molecule complementary to the aptamer.
  • the nucleic acid molecule complementary to the aptamer is, for example, the same as in Embodiment 4A.
  • the labeling substance in the labeled second binding substance is preferably, for example, a catalytic nucleic acid molecule or an enzyme exhibiting a catalytic function, and the labeling substance, the catalytic nucleic acid molecule, and the enzyme are the same as in, for example, Embodiment 4A .
  • the carrier in the immobilized first binding substance may be, for example, a bead or the inner wall of the second chamber.
  • the beads are the same as in Embodiment 4A, for example.
  • the amount of aptamer immobilized on the carrier (bead) is not particularly limited. For example, 0.1 fmol to 100 pmol, 1 fmol to 10 pmol, 10 fmol to 1 pmol per 1 mm 2 of the surface area of the bead. It is.
  • the carrier is the inner wall of the second chamber
  • the amount of aptamer immobilized on the carrier (inner wall) is not particularly limited. For example, 0.1 fmol per 1 mm 2 area of the inner wall of the second chamber ⁇ 100 pmol, 1 fmol ⁇ 10 pmol, 10 fmol ⁇ 1 pmol.
  • aptamers can be used as the first binding substance and complementary nucleic acid molecules can be used as the second binding substance, for example, it is heat-stable and is easier to store.
  • an enzyme such as luciferase, alkaline phosphatase or peroxidase can be used as the labeling substance, for example, the target can be analyzed with high sensitivity.
  • the second chamber may further include an adsorption carrier that adsorbs at least one of protein and lipid as the second reagent.
  • the adsorption carrier is the same as that in the embodiment 4A, for example.
  • This embodiment is an embodiment of the analysis method of (D) in which the first reagent and the second reagent use the analysis tool of the combination (4) and the analysis tool of the combination (4).
  • the analysis tool of Embodiment 4D includes the analysis container of the present invention, a first reagent, and a second reagent
  • the first chamber includes, as the first reagent, an immobilized first binding substance in which a first binding substance that binds to a target is fixed to a carrier
  • the second chamber includes, as the second reagent, a labeled second binding substance in which a labeling substance is bound to a second binding substance that binds to the first binding substance
  • the third chamber is a detection unit for detecting the labeled second binding substance
  • the second partition wall is a porous partition wall through which the immobilized first binding substance cannot pass and the labeled second binding substance can pass through.
  • the labeled second binding substance bound to the immobilized first binding substance remains in the second chamber without moving to the third chamber.
  • the free labeled second binding substance that has not been bound to the immobilized first binding substance passes through the partition wall and is introduced into the third chamber. Since the labeled second binding substance in the analysis tool of the present embodiment can be, for example, a known amount, the amount of the labeled second binding substance unbound to the immobilized first binding substance is This indirectly corresponds to the target amount in the sample. Therefore, the presence or amount of the target in the sample can be indirectly analyzed by detecting the unbound labeled second binding substance introduced into the third chamber.
  • the analysis container of Embodiment 1 is used as the analysis container, and the aptamer is used as the first binding substance
  • the analysis container of Embodiment 1 is used as the analysis container
  • the aptamer is used as the first binding substance
  • Examples of the labeling substance in the labeled second binding substance include a catalytic nucleic acid molecule and an enzyme that exhibit a catalytic function, and the labeling substance, the catalytic nucleic acid molecule, and the enzyme are, for example, each of Embodiment 4A. It is the same.
  • the second partition between the second chamber and the third chamber is a porous partition through which the immobilized first binding substance cannot pass and the labeled second binding substance can pass.
  • the pore diameter of the second partition wall can be appropriately set according to the size of the immobilized first binding substance and the labeled second binding substance, for example.
  • the hole diameter in the second partition wall is, for example, 0.2-100 ⁇ m, 0.2-50 ⁇ m, 0.5-10 ⁇ m.
  • the first chamber may further include, for example, an extract for extracting the components inside the sample.
  • an extract for extracting the components inside the sample.
  • the extract is the same as in Embodiment 4A.
  • the second chamber may further include an adsorption carrier that adsorbs at least one of protein and lipid as the second reagent.
  • the adsorption carrier is the same as that in the embodiment 4A, for example.
  • the third chamber preferably further contains, for example, a substrate for its catalytic function.
  • the substrate is the same as that in Embodiment 4A, for example.
  • FIG. 11 is a schematic view showing how to use the analytical tool 7.
  • the sample holding tool 20 in which the sample is held in the holding unit 202 is inserted into the first chamber 11 of the analysis tool 7, and the target 21 in the sample is bound to the aptamer 281 of the immobilized aptamer 28 in the first chamber 11.
  • the treatment conditions for the combination of the two are not particularly limited, and the temperature is, for example, 4 to 45 ° C., and the time is, for example, 10 seconds to 10 minutes.
  • the combination of the two is preferably performed in, for example, a liquid solvent, and examples of the liquid solvent include aqueous solvents such as water, buffer solution, physiological saline, and a mixed solution thereof.
  • the first partition 111 is broken by the sample holding tool 20, and the contents in the first chamber 11 are introduced into the second chamber 12. Then, the labeled complementary strand 29 is bound to the aptamer 281 that is not bound to the target 21 among the aptamers 281 immobilized on the beads 282. Further, a sealing member 151 is disposed on the outer surface of the third chamber 13 so as to cover the through hole 14 during the coupling process of the both.
  • the treatment conditions for the combination of the two are not particularly limited, and the temperature is, for example, 4 to 45 ° C., and the time is, for example, 10 seconds to 30 minutes.
  • the bonding between the two is preferably performed, for example, in the liquid solvent.

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Abstract

L'invention concerne un récipient d'analyse dans lequel un liquide peut s'écouler d'une manière régulée, un dispositif d'analyse de cible l'utilisant et un procédé d'analyse de cible. Ce récipient d'analyse est caractérisé en ce qu'il comprend une première chambre 11, une deuxième chambre 12 et une troisième chambre 13 et en ce que : la première chambre 11, la deuxième chambre 12 et la troisième chambre 13 sont agencées de manière séquentielle dans cet ordre ; le récipient d'analyse présente une première cloison 111 entre la première chambre 11 et la deuxième chambre 12 ; le récipient d'analyse présente une deuxième cloison 121 entre la deuxième chambre 12 et la troisième chambre 13 ; un instrument de support d'échantillon peut être introduit à l'intérieur de la première chambre 11 depuis l'extérieur ; la première paroi de séparation 111 se rompt suite à un contact avec la pointe de l'instrument de support d'échantillon, qui a été introduit dans la première chambre 11 ; la deuxième cloison 121 est une cloison poreuse ; la troisième chambre 13 présente au moins un trou traversant 14 ; un élément de fermeture pouvant être ouvert/fermé est disposé sur le trou traversant 14 pour recouvrir le trou traversant ; et l'élément de fermeture est enlevé pour ouvrir le trou traversant 14 afin de permettre l'écoulement du liquide depuis la deuxième chambre 12 vers la troisième chambre 13.
PCT/JP2016/087698 2016-03-31 2016-12-19 Récipient d'analyse, instrument d'analyse de cible l'utilisant et procédé d'analyse de cible Ceased WO2017168876A1 (fr)

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Cited By (1)

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WO2019181400A1 (fr) * 2018-03-22 2019-09-26 富士フイルム株式会社 Méthode de traitement d'échantillon et récipient pour traitement d'échantillon

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JP2006231166A (ja) * 2005-02-23 2006-09-07 Fukae Kasei Kk 液体試料の処理用器具及びその使用方法
JP2011247851A (ja) * 2010-05-31 2011-12-08 Shin Corporation Co Ltd 検査用キット
JP2012513773A (ja) * 2008-12-31 2012-06-21 スリーエム イノベイティブ プロパティズ カンパニー 微小粒子を用いた生きた生物負荷の検出法
JP2012132897A (ja) * 2010-11-30 2012-07-12 Shizuokaken Koritsu Daigaku Hojin 検体採取具及び検体採取キット
WO2016117701A1 (fr) * 2015-01-22 2016-07-28 Ｎｅｃソリューションイノベータ株式会社 Appareil d'analyse de cible et procédé d'analyse de cible

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006231166A (ja) * 2005-02-23 2006-09-07 Fukae Kasei Kk 液体試料の処理用器具及びその使用方法
JP2012513773A (ja) * 2008-12-31 2012-06-21 スリーエム イノベイティブ プロパティズ カンパニー 微小粒子を用いた生きた生物負荷の検出法
JP2011247851A (ja) * 2010-05-31 2011-12-08 Shin Corporation Co Ltd 検査用キット
JP2012132897A (ja) * 2010-11-30 2012-07-12 Shizuokaken Koritsu Daigaku Hojin 検体採取具及び検体採取キット
WO2016117701A1 (fr) * 2015-01-22 2016-07-28 Ｎｅｃソリューションイノベータ株式会社 Appareil d'analyse de cible et procédé d'analyse de cible

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019181400A1 (fr) * 2018-03-22 2019-09-26 富士フイルム株式会社 Méthode de traitement d'échantillon et récipient pour traitement d'échantillon

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