WO2016191293A1 - Prediction of hybrid vigor using circadian-regulated stress-responsive gene expression - Google Patents

Abstract

Methods for producing hybrid plants exhibiting heterosis are provided, including methods for selecting parent plants for a cross to produce hybrid progeny plant having advantageous properties such as increased biomass and increased yield. Plants, plant parts, plant cells, and seeds resulting from the methods are further provided.

Description

PREDICTION OF HYBRID VIGOR USING ORCADIAN-REGULATED

STRESS-RESPONSIVE GENE EXPRESSION

REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of United States Provisional Application 62/165,637, filed May 22, 2015, which is incorporated herein by reference in its entirety.

STATEMENT OF GOVERNMENT INTEREST

[0002] This invention was made with government support under Grant Number ISO1238048 awarded by the National Science Foundation. The government has certain rights in the invention.

INCORPORATION OF SEQUENCE LISTING

[0003] A sequence listing contained in the file named "UTTA003WO_ST25.txt" which is 9.73 bytes (measured in MS-Windows®) and created on May 20, 2016, comprises 54 nucleotide sequences, is filed electronically herewith and incorporated by reference in its entirety.

BACKGROUND

[0004] Hybrid plants and animals, such as corn and domestic dogs, grow larger and more vigorously than their parents, a common phenomenon known as hybrid vigor or heterosis. Since Charles Darwin systematically described the phenomenon, heterosis has been widely applied in agriculture to dramatically improve the production of crops and farm animals. Heterosis in food crops often refers to grain yield, while biomass heterosis is commonly measured in other crops including vegetable and energy crops. However, the molecular basis for heterosis remains elusive, and traditional dominance and overdominance genetic models cannot adequately explain heterosis in yeast and many crop plants. To this end, the conventional technology uses phenotypes and DNA sequence polymorphism as selection criteria to make high-yield hybrids. The predictability resulting from this conventional technology is relatively low.

SUMMARY

[0005] In one aspect, the present invention provides methods of producing a hybrid plant with increased biomass comprising the steps of: (a) detecting an expression level of a stress- responsive gene in a plurality of plants; (b) selecting a first parent plant having a first expression level of the stress-responsive gene from the plurality of plants; (c) selecting a second parent plant of a different genotype having a second expression level of the stress- responsive gene from the plurality of plants, wherein the second expression level is different from the first expression level; and (d) crossing the first parent plant with the second parent plant to produce a progeny plant. In certain embodiments, the stress-responsive gene is an abiotic stress gene, for example LSR3, ERD11/GSTF6, COR47/RD17, LTI45/ERD10, COR413-TM1, ALDH7B4, ATGRP3, PIP2B, RD28, CAX1/RCI4, COR15A, PIP1B, LTI30, PIP1A, PIP1;4, COR78/RD29A, COR6.6/KIN2, RD22, ERD1, GRP8, GRP2, GRP7/CCR2, MDH, HSP70 family, CAT2, LOS2, ATAVP1, LTP4, TCH2, TCH4, mtHsc70-l, COR15B, MT1A, LTP3, LOS1, Salt-stress responsive protein, FIB, KIN1, or homologs thereof. In other embodiments, the stress-responsive gene is a biotic stress gene, for example PR1, PR5, NUDT6, HSPR02, CNGC11, EBP/ERF72, PCC1, HR3/MLA10, CAX3, PAD4, WRKY70, PR2/BGL2, ACD6, NIA2, NHL3, Protease inhibitor, WAK1, WAK2, EP1, RVE2, BG3, LURP1, DMR6, SAG21, SUR2, ANK, or homologs thereof.

[0006] In some embodiments of the invention, the expression level of the stress-responsive gene is detected at one or more time points selected from the group consisting of zeitgeber time (ZT) 0, ZT6, ZT12, ZT15, ZT18, and ZT21. The ratio of the first gene expression level to the second gene expression level may be greater than 1.00, for example greater than 1.5, or greater than 3.0. In further embodiments, the expression levels of stress-responsive genes are detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR).

[0007] In further embodiments, the invention provides methods for producing hybrid progeny plants wherein the progeny plant exhibits and improved agronomic trait compared with the parent plants, for example biomass, yield, disease tolerance, insect resistance, pathogen resistance, plant growth and development, starch content, oil content, fatty acid content, protein content, fruit ripening, and stress resistance.

[0008] Embodiments of the present invention include methods for use with monocot plants or dicot plants, for example plants is selected from the group consisting of: Arabidopsis thaliana, maize (corn; Zea mays), soybean (Glycine max), cotton (Gossypium hirsutum; Gossypium sp.), peanut (Arachis hypogaea), barley (Hordeum vulgar e); oats (Avena sativa); orchard grass (Dactylis glomerata); rice (Oryza sativa, including indica and japonica varieties); sorghum (Sorghum bicolor); sugar cane (Saccharum sp.); tall fescue (Festuca arundinacea); turfgrass species (e.g. species: Agrostis stolonifera, Poa pratensis, Stenotaphrum secundatum); wheat (Triticum aestivum); alfalfa (Medicago sativa); members of the genus Brassica, including broccoli, cabbage, carrot, cauliflower, Chinese cabbage; cucumber, dry bean and other leguminous plants, eggplant, tobacco (Nicotiana sp.), fennel, garden beans, gourd, leek, lettuce, melon, okra, onion, pea, pepper, pumpkin, radish, spinach, squash, sweet corn, tomato, potato, watermelon, Miscanthus, ornamental plants, and other fruit, vegetable, tuber, oilseed, and root crops, wherein oilseed crops may include soybean, canola, oil seed rape, oil palm, sunflower, olive, corn, cottonseed, peanut, flaxseed, safflower, and coconut.

[0009] In another aspect, the invention provides a progeny plant produced by the methods of the invention, or a plant part, plant cell, or seed of such a progeny plant.

[0010] In further embodiments, the invention provides methods of producing a hybrid plant with increased biomass comprising the steps of: (a) detecting an expression level of at least two stress-responsive genes in a plurality of plants; (b) selecting a first parent plant having a first expression level of each of the stress-responsive genes from the population of plants; (c) selecting a second parent plant of a different genotype having a second expression level of each of the stress-responsive gene from the population of plants, wherein the first expression level for each gene is different than the second expression level for that gene; and (d) crossing the first parent plant with the second parent plant to produce a progeny plant.

[0011] The present disclosure provides methods of selecting parents for producing hybrids with high-yield biomass based on rhythmic stress-responsive gene expression. In one embodiment, a method for producing a hybrid plant with high-yield biomass comprising the following steps of measuring gene expression for a stress-responsive gene in a population of plants under both a stress condition and a non-stress condition. Based on the gene expression of the stress responsive gene, a normal gene expression profile is then determined for the population of plants. The normal gene expression profile comprises a first expression level based on the expression of the stress-responsive gene during the non-stress condition and a second expression level based on the expression of the stress-responsive gene during the stress condition. A first plant is then selected based on display of a lower expression of the stress-responsive gene during the non-stress condition as compared to the first expression level and the same or higher expression of the stress-responsive gene during the stress condition as compared to the second expression level. This first plant is then crossed with a second plant to produce the hybrid plant.

[0012] In another embodiment, the second plant can be selected in a similar fashion as the first plant based on expression of a second stress-responsive gene. In this embodiment, gene expression for a second stress-responsive gene is measured in a second population of plants under both a second stress condition and a second non-stress condition. A second normal gene expression profile based on the second population of plants collectively is then developed, wherein the second normal gene expression profile comprises a third expression level based on the expression of the second stress-responsive gene during the second non- stress condition and a fourth expression level based on the expression of the second stress- responsive gene during the second stress condition. Finally, the second plant is selected based on its lower expression of the second stress-responsive gene during the second non- stress condition as compared to the third expression level and the same or higher expression of the second stress-responsive gene during the second stress condition as compared to the fourth expression level. In this embodiment, the first plant may display a higher expression of the second stress-responsive gene during the second non-stress condition as compared to the second plant and the same or lower expression of the second stress-responsive gene during the second stress condition as compared to the second plant. Similarly, the second plant may display a higher expression of the first stress-responsive gene during the first non- stress condition as compared to the first plant and the same or lower expression of the first stress-responsive gene during the first stress condition as compared to the first plant.

[0013] In the above embodiments, the first stress condition is abiotic and the second stress condition is biotic. Alternatively, the first stress condition is biotic and the second stress condition is abiotic.

[0014] A hybrid plant is also provided. In one embodiment, the hybrid plant is derived from a first parent and a second parent, wherein the first parent displays a first expression level of a abiotic stress-responsive gene and a second expression level of a biotic stress-responsive gene, wherein the second parent displays a third expression level of the abiotic stress- responsive gene and a fourth expression level of the biotic stress-responsive gene, wherein the first expression level is higher than the third expression level, and wherein the second expression level is lower than the fourth expression level. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 provides a comparison of expression of many abiotic and biotic stress- responsive genes in reciprocal Fl hybrids relative to the parents (by convention, the female is listed first in a genetic cross), (a, c) Rosettes of 3-week-old plants (scale bars = 1 cm) (a) and the tenth leaf stained with Trypan blue (scale bars = 0.5 mm) (c). (b, d) Clustered heatmaps of RNA-seq data (Table 7) showing subsets of genes in biotic (b) and abiotic (d) stress (ZTO = dawn). Fold-change: Log2-transformed values from low to high, (e-j) Percentage of down- regulated differentially expressed genes that are biotic or abiotic stress-responsive genes at ZTO (e, f), ZT6 (g, h), and ZT15 (i, j) in reciprocal Fl hybrids ColXC24 (e, g, i) and C24XCol (f, h, j).

[0016] Figure 2 relates to the diurnal regulation of gene expression of ACD6 and COR78 in two ecotypes, Col and C24, and in hybrids (arrows), (a, b) Relative expression levels (R.E.L.) of ACD6 (a) and COR78 (b) every 3 hours in a 48-hour period (ZT 0 = dawn) (mean+s.d., values were averaged from three biological replicates). Single and double asterisks indicate statistical significance levels at p<0.05 and p<0.01 using two-tailed student' s t-test (compared to MPV), respectively, (c, d) Bioluminescent activities (counts per second in 1,000) of transgenic (T2) seedlings that expressed ACD6(Col):LUC in Col (dark gray) or ACD6(C24):LUC in C24 (light gray) (c) and ACD6(C24):LUC in Col (dark gray) or ACD6(Col):LUC in C24 (light gray) (d). (e, f) Bioluminescent activities of transgenic seedlings that expressed COR78(Col):LUC in Col (light gray) or COR78(C24):LUC in C24 (dark gray) (e) and COR78(Col):LUC in C24 (light gray) or COR78(C24):LUC in Col (dark gray) (f). Diurnal cycles of light (open) and dark (filled) and one of three replicate experiments (mean+s.e.m., n=16 individuals per transgenic line, 3 independent transgenic lines were examined) are shown.

[0017] Figure 3 provides data to support circadian clock regulation of the rhythmic expression of ACD6 and COR78. (a, b) Bioluminescent activities of pACD6(Ws):LUC in T3 transgenic seedlings that were treated with salicylic acid (SA) and the control (a) and of pCOR78(Ws):LUC in T3 transgenic seedlings that were treated with cold and the control (b). (c, d) Altered bioluminescent activities of pACD6(Ws):LUC in T3 transgenic seedlings in ccal-11, lhy21, ccal-11 lhy21, and Ws backgrounds, which were sprayed with water (control, c) or SA (d). (e, f) Altered bioluminescent activities of pCOR78(Ws):LUC in T3 transgenic seedlings in ccal-11, lhy21, ccal-11 lhy21, and Ws backgrounds, which were subjected to control (e) or cold (f) treatments. One of three replicate experiments (χΙΟ,ΟΟΟ counts per second+ s.e.m., n=16 individuals per transgenic line, 3 independent transgenic lines were examined) is shown.

[0018] Figure 4 provides data to demonstrate Stress-responsive gene expression levels as predictors for heterosis and effects of cold stress and salicylic acid (SA) on the growth rate in hybrids and their parents, (a, b). Relative expression levels (R.E.L.) of ACD6 (a) and COR78 (b) in 10 ecotypes at ZTO, ZT9, and ZT18. Values are averages from three biological replicates (+ s.d.). C24 (dashed-box) has higher ACD6 and lower COR78 expression levels than Col, Ler, and Ws (grey-boxes), (c, d, e) Biomass increase relative to MPV (Y-axis) in various hybrids was plotted against absolute values of the log2-fold expression level changes (X-axis) in different stress genes at three time points (ZTO, 9, 18). Scatter plots for ACD6 at ZT18 (c), COR78 at ZT9 (d), and COR47 at ZT9 (e). Regression lines were statistically significant for ACD6 at ZT18 (c), COR78 at ZT9 (d) and COR47 at ZT9 (e). Data used to perform regression analyses are shown in Table 4, and detailed results of the analysis were included in Table 8. (f, g) Rosette diameters of seedlings (n=15 plants averaged per replicate) that were grown at 22oC (f) or 4oC (g) for 17 days and transferred to grow in soil for another 8 days at 22oC. (h, i) Rosette diameters of seedlings (n=15 plants averaged per replicate) that were mock treated (h) or treated with salicylic acid twice (indicated by arrows) (SA) (i) and were transferred to soil for another 8 days. Values were averaged from three biological replicates (mean+ s.d.). Single and double asterisks indicate statistically significant levels of p<0.05 and p<0.01 using two-tailed student's t-test (compared to MPV), respectively.

[0019] Figure 5 provides data demonstrating the effects of knockdown or overexpression of abiotic and biotic genes on biomass. (a) Rosettes of 3-week-old plants in Fl(C24xLer) and its parents C24 and Ler. (b) Rosettes of 3 week-old plants in Fl(C24xWs) and its parents C24 and Ws. Scale bars = 1 cm for all images, (c-f) Biomass of rosettes from 3-week-old seedlings in high-vigor crosses C24XLer (c) and C24XWs (d) and low-vigor crosses ColXLer (e) and ColXWs (f). Values were averaged from three biological replicates (+ s.d.). (g) Biomass of Col and acd6-l hyperactive mutant, (h, i) Dry weight (mg) of transgenic amiRACD6 lines in Col (h) and in C24 (i). (j, k) Dry weight (mg) of transgenic amiRCOR78 lines in Col (j) and in C24 (k). (1, m) Dry weight (mg) of transgenic 35S:COR78 lines in Col (1) and in C24 (m). (n-p) Relative expression levels of COR78 expression in overexpression lines (n) and in amiRNA lines (o) and of ACD6 in amiRNA lines (p). Values were averaged from three biological replicates (+ s.d.). Single and double asterisks indicate statistical significance levels at p<0.05 and p<0.01 using two-tailed student's t-test, respectively, compared to the transgenic control (vector).

[0020] Figure 6 provides an analysis of DNA methylation in stress-related genes, (a, b) Sequence densities and distributions of CG, CHG, and CHH (H = A, T, or C) methylation, small RNA, and mRNA in the vicinity of PR1 (a) and ACD6 (b) genomic regions. Gray boxes are genes, and black boxes are transposons. Changes in CHG and CHH methylation between Fl reciprocal hybrids and their parents (Ler and C24) are boxed. Genomic coordinates are shown above each diagram, (c-e) Percentage (%, Y-axis) of CG (c), CHG (d), and CHH (e) methylation (H = A, T, or C) levels in all genes, (f-h) Percentage (%, Y-axis) of CG (f), CHG (g), and CHH (h) methylation levels in all biotic stress responsive genes, (i-k) Percentage (%, Y-axis) of CG (i), CHG (j), and CHH (k) methylation levels in all abiotic stress responsive genes. X-axis: positions relative to the transcribed region.

[0021] Figure 7 provides a proposed model for altered stress-responsive gene expression in the promotion of growth vigor in Fl hybrids, (a) An example of an Fl (ColXC24) hybrid showing growth vigor compared to the parents, Col and C24 (scale bars=l cm). Natural variation of circadian rhythms and stress responses is associated with the adaptation of each ecotype to local environments, (b) Parent 1 and parent 2 that have adapted to different environments require high levels of gene expression in response to abiotic (parent 1) or biotic (parent 2) stress, which is partly mediated by circadian rhythms (clock symbols). Parent 1 and parent 2 do not reach the full growth potential, probably because of the fitness cost in response to the stress in respective adaptive environments. In Fl hybrids, the expression of both biotic and abiotic stress-responsive genes is compromised, leading to increased levels of growth vigor.

[0022] Figure 8 shows an analysis of differentially expressed genes (DEGs) in hybrids, (a-f) Only significantly enriched GO terms are displayed. Enriched GO categories are defined by having a FDR adjusted p<0.05 using the hypergeometric test (compared to that of all genes in the genome), (g) Heatmap of RNA-seq data (Table 7) showing a subset of genes in the photosynthetic pathway (ZT0 = dawn). Fold-change: Log₂-transformed values from low to high, (h-k) Relative expression levels (R.E.L.) of LHCB1.4 (h), PORB (i), CAB2 (j), and LHCB4.2 (k) at 4 time points in reciprocal Fl hybrids between Col and C24, compared to the mid-parent value (MPV). Values were averaged from three biological replicates (+ s.d.). Single and double asterisks indicate statistical significance levels at p<0.05 and p<0.01, respectively, using two-tailed student's t-test (compared to MPV). Arrows indicate up- regulation in the hybrids compared to the MPV.

[0023] Figure 9 shows biotic and abiotic stress genes exhibiting altered expression in Fl hybrids, (a-h) Expression of PCC1 (a), HSPOR2 (b), PR1 (c), CPR5 (d), COR47 (e), COR15A (f), RD22 (g), and RD28 (h) in a 48-hour period starting from dawn. R.E.L.: relative expression levels to that of ACT7 in each sample. Values were averaged from three biological replicates (+ s.d.). Single and double asterisks indicate statistical significance levels at p<0.05 and p<0.01, respectively, using two-tailed student's t-test (compared to the mid-parent value, MPV). Arrows indicate down-regulation in hybrids compared to the MPV.

[0024] Figure 10 shows cis and trans effects on stress genes in Fl hybrids, (a) Number of genes with cis and trans effects in Fl hybrids, (b) Percentage of stress-responsive genes with cis or trans effects. Statistically significant enrichment of stress genes is indicated by asterisks, which are representative of a FDR adjusted p<0.05 using the hypergeometric test (compared to the whole genome background), (c-h) Clustered heatmaps showing RPKM of trans -effected genes in parents and hybrid alleles, (i-p) RPKM values in Col, C24, and Col and C24 alleles in reciprocal hybrids for ACD6 (i), PR1Q), PR2/BGL2 (k), PAD4 (1), COR78 (m), COR15A (n), COR47 (o) and RD22 (p) at ZTO (i-1) or ZT6 (m-p).

[0025] Figure 11 shows perturbation of circadian-regulated genes in Fl hybrids, (a-c) Relative expression levels (R.E.L.) of CCA1 (a), LHY (b), and TOC1 (c) in a 24-h period starting from dawn (ZT = 0) in reciprocal Fl hybrids between C24 and Col. Values were averaged from three biological replicates (+ s.d.). Single and double asterisks indicate statistical significance levels at p<0.05 and p<0.01, respectively, using two-tailed student's t- test (compared to the mid-parent value, MPV). Upward and downward arrows indicate up- and down-regulation in the hybrids compared to the MPV. (d-i) Phase distributions of differentially expressed genes (DEGs) in hybrids in ColxC24 (d, f, h) and C24xCol (e, g, i) at ZTO, ZT6, and ZT15. Dark gray and light gray lines indicate down- and up-regulated genes, respectively. P- values calculated using chi-square test of independence. [0026] Figure 12 shows natural variation of stress responsive gene expression at specific times of day is associated with biomass heterosis, (a) Seedlings of 3-week-old plants in the following ecotypes: Cvi, Est, Ler, Oy, Col, Sorbo, Ws, C24, Nd, and Wei (scale bars = 1 cm for all images), (b) Biomass (dry weight, mg) of the ecotypes in three replications (mean + s.d.). (c-d) Relative expression levels (R.E.L) PR1 (c) and COR47 (d) in ecotypes at ZTO, ZT9, and ZT18. Values are averages from three biological replicates (+ s.d.). (e-p) Biomass increase relative to MPV (Y-axis) in various hybrids was plotted against absolute values of the log₂-fold expression level changes (X-axis) in different stress genes at three time points (ZTO, 9, 18). Scatter plots for ACD6 (e-g), COR78 (h-j), PR1 (k-m), and COR47 (n-p) were shown at ZTO (e, h, k, n), ZT9 (f, i, 1, o), and ZT18 (g, j, m, p). Regression lines were statistically significant for ACD6 at ZT18 (g), COR78 at ZT9 (i) and COR47 at ZT9 (o). Data used to perform regression analyses were shown in Table 4, and detailed results of the analysis were included in Table 8.

[0027] Figure 13 shows responsive gene expression in higher and lower vigor Fl hybrids, (a- d) Relative expression levels (R.E.L.) of COR78 and ACD6 in Fl(ColXWs) and its parents (a, c) and in Fl(ColXL<?r) and its parents (b, d) at 5 time points, compared to the mid-parent value (MPV). Values were averaged from three biological replicates (+ s.d.). (e, f) Seedlings of 3-week-old plants (scale bars = 1 cm for all images) in Fl(ColXWs) and its parents Col and Ws (e) and in (ColXL<?r) and its parents Col and Ler (f). (g-j) Relative expression levels (R.E.L to that of ACT7) of COR78 and ACD6 in C24 and Ler and their Fl (g, i) and in C24 and Ws and their Fl (h, j) at 5 time points, compared to the mid-parent value (MPV). Values were averaged from three biological replicates (+ s.d.). (k) Seedlings of 3-week-old plants in Fl(EstXCol) and its parents Est and Col. (1, m) R.E.L. of COR78 (1) and ACD6 (m) in Fl(EstlXCol) and its parents at 5 time points, compared to the mid-parent value (MPV). Single and double asterisks indicate statistical significance levels at p<0.05 and p<0.01, respectively, using two-tailed student's t-test (compared to MPV). Upward and downward arrows indicate up- and down-regulation in the hybrids compared to the MPV.

[0028] Figure 14 shows an experimental scheme for stress treatments. All experiments were performed in a 16-hour light/8-hour dark regime. The white box represents light and the black box represents dark, (a, b) Experimental design for measurement of gene expression induction at ZTO (a) and ZT15 (b), which was treated by either cold shock or salicylic acid (SA). (c, d) Experimental design for longer-term stress applications under the treatment of cold (4 °C) for two weeks (c) or SA spray (d).

[0029] Figure 15 shows performance and stress-responsive gene expression in Fl(ColXC24) hybrids and their parents (Col and C24). (a-d) Relative expression ratios (R.E.R.) of COR78 (a, b) and COR15A (c, d) between treated and untreated samples across 5 time points after the cold-treatment at ZTO (a, c) or ZT15 (b, d). (e-h) R.E.R. of ACD6 (e, f) and PR1 (g, h) between treated and untreated samples across 5 time points after the SA-treatment at ZTO (e, g) or ZT15 (f, h). Expression values were averaged from three biological replicates (+ s.d.). Asterisks indicate statistical significance levels at p<0.05 using two-tailed student's t-test, compared to the mid-parent value (MPV). Dashed lines indicate no expression change between treated and untreated samples. After seedlings were cold- or SA-treated for 1 hour at ZTO or ZT15, rosette leaves were harvested at designated time points for gene expression studies (see Methods for details), (i) Dry weight (n = 5 plants averaged per replicate) of the hybrids relative to the MPV in the control and cold-treated conditions 8 days post transfer, (j) Dry weight (n = 5 plants averaged per replicate) of the hybrids relative to the MPV in the control and SA-treated conditions after 15 days. Relative expression ratios (R.E.R.) of COR78 (k), COR15A (1), ACD6 (m), and PR1 (n) between treated and control samples across 3 time points after 15 days of stress treatment. Dashed lines indicate no expression change between treated and untreated samples. Expression values are averaged from three biological replicates (+ s.d.). Asterisks indicate statistical significance levels at p<0.05 using two-tailed student's t-test, compared to the mid-parent value (MPV). Values were averaged from three biological replicates (+ s.d.).

DETAILED DESCRIPTION

[0030] Hybrid plants exhibit enhanced agronomic traits due to the varied genetic contribution of their parents, a phenomenon known as heterosis or outbreeding enhancement. While useful hybrids have been identified through extensive breeding and evaluation of the resulting phenotypes, this process is costly and time-consuming. Efforts to develop methods of selecting parents for producing hybrid plants with beneficial traits have been hampered by an incomplete understanding of the factors contributing to heterosis. A need therefore remains for improved methods of producing hybrid plants with useful phenotypes. In the absence of improved methods for selecting parent plants for hybrid breeding programs, it may not be practical to attempt to produce certain new hybrid crop plants.

[0031] The present invention provides previously unknown methods for producing hybrid plants with significantly improved agronomic traits. In certain embodiments, methods are provided for selecting parent plants for crossing based on expression levels of stress- responsive genes, which result in hybrid plants exhibiting enhanced traits due to heterosis. The methods of the present invention can therefore be used to accurately predict advantageous traits in hybrid plants, including increased biomass or increased yield.

I. Methods for Producing Hybrid Plants

[0032] The present invention provides methods of producing hybrid plants with improved agronomic traits comprising selecting parent plants based on expression levels of stress- responsive or photosynthetic genes. In certain embodiments, the invention provides methods comprising detecting expression levels of one or more stress-responsive genes in a plurality of plants, and selecting parent plants for a cross which express the stress-responsive genes at different levels. For example, a first parent plant may express high levels of a stress- responsive gene, while a second parent plant expresses low levels of the stress-responsive gene. In another example, a first parent plant may express low levels of a stress-responsive gene, while a second parent plant expresses high levels of the stress-responsive gene. The plurality of plants from which the parent plants are selected according to the methods of the present invention may be from the same species of plant, and may comprise different genotypes.

[0033] According to the methods provided herein, parent plants for a hybrid cross may be selected by determining a difference in expression level of a particular gene between prospective parent plants. Genes useful in selecting parent plants for crossing according to the invention include any stress-responsive gene. For example, parent plants may be selected by detecting a difference in expression levels of abiotic stress genes, biotic stress genes, or photosynthetic genes. Gene expression levels may be detected and compared between prospective parent plants at any timepoint, including zeitgeber time (ZT) 0, ZT6, ZT12, ZT15, ZT18, and ZT21. In certain embodiments of the invention, parent plants having ratios of stress-responsive gene expression of 1.00, 1.50, or 2.00 are predictive of improved agronomic traits in progeny plants. [0034] The present invention further provides methods of producing hybrid plants having beneficial traits by detecting and comparing expression levels of one or more stress- responsive genes to select parent plants for a cross. A difference in expression levels of one or more stress-responsive genes can be used to select parent plants for a cross resulting in hybrid plants with advantageous traits.

II. Genes Useful in Selecting Parent Plants

[0035] The methods of the present invention may comprise detecting expression levels of any stress-responsive gene, including biotic stress genes and abiotic stress genes. Non-limiting examples of biotic stress genes include PRl (AT2G14610), PR5 (AT1G75040), NUDT6 (AT2G04450), HSPR02 (AT2G40000), CNGC11 (AT2G46440), EBP/ERF72 (AT3G16770), PCC1 (AT3G22231), HR3/MLA10 (AT3G50470), CAX3 (AT3G51860), PAD4 (AT3G52430), WRKY70 (AT3G56400), PR2/BGL2 (AT3G57260), ACD6 (AT4G14400), NIA2 (AT1G37130), NHL3 (AT5G06320), Protease inhibitor (AT5G55450), WAK1 (AT1G21250), WAK2 (AT1G21270), EP1 (AT4G23170), RVE2 (AT5G37260), BG3 (AT3G57240), LURP1 (AT2G14560), DMR6 (AT5G24530), SAG21 (AT4G02380), SUR2 (AT4G31500), ANK (AT5G54610), and homologs thereof.

[0036] Non-limiting examples of abiotic stress genes useful in the present invention include LSR3 (AT1G01470), ERD11/GSTF6 (AT1G02930), COR47/RD17 (AT1G20440), LTI45/ERD10 (AT1G20450), COR413-TM1 (AT1G29395), ALDH7B4 (AT1G54100), ATGRP3 (AT2G05520), PIP2B (AT2G37170), RD28 (AT2G37180), CAX1/RCI4 (AT2G38170), COR15A (AT2G42540), PIP1B (AT2G45960), LTI30 (AT3G50970), PIP1A (AT3G61430), PIP1;4 (AT4G00430), COR78/RD29A (AT5G52310), COR6.6/KIN2 (AT5G15970), RD22 (AT5G25610), ERD1 (AT5G51070), GRP8 (AT4G39260), GRP2 (AT4G38680), GRP7/CCR2 (AT2G21660), MDH (AT3G47520), HSP70 family (AT3G09440), CAT2 (AT4G35090), LOS2 (AT2G36530), ATAVP1 (AT1G15690), LTP4 (AT5G59310), TCH2 (AT5G37770), TCH4 (AT5G57560), mtHsc70-l (AT4G37910), COR15B (AT2G42530), MT1A (AT1G07600), LTP3 (AT5G59320), LOS1 (AT1G56070), Salt-stress responsive protein (AT1G13930), FIB (AT4G04020), KIN1 (AT5G15960), and homologs thereof.

[0037] Hybrid plants produced by the novel methods of the present invention therefore exhibit enhanced agronomic traits including modified biomass, modified yield, improved disease tolerance, improved insect resistance, improved pathogen resistance, modified plant growth and development, modified starch content, modified oil content, modified fatty acid content, modified protein content, modified fruit ripening, and improved stress resistance.

[0038] The methods provided by the invention may be used to produce hybrid plants exhibiting advantageous traits resulting from heterosis in monocotyledonous or dicotyledonous plants including, but not limited to, Arabidopsis thaliana, maize (corn; Zea mays), soybean (Glycine max), cotton (Gossypium hirsutum; Gossypium sp.), peanut (Arachis hypogaea), barley (Hordeum vulgare); oats (Avena sativa); orchard grass (Dactylis glomerata); rice (Oryza sativa, including indica and japonica varieties); sorghum (Sorghum bicolor); sugar cane (Saccharum sp.); tall fescue (Festuca arundinacea); turfgrass species (e.g. species: Agrostis stolonifera, Poa pratensis, Stenotaphrum secundatum); wheat (Triticum aestivum); alfalfa (Medicago sativa); members of the genus Brassica, including broccoli, cabbage, carrot, cauliflower, Chinese cabbage; cucumber, dry bean and other leguminous plants, eggplant, tobacco (Nicotiana sp.), fennel, garden beans, gourd, leek, lettuce, melon, okra, onion, pea, pepper, pumpkin, radish, spinach, squash, sweet corn, tomato, potato, watermelon, Miscanthus, ornamental plants, and other fruit, vegetable, tuber, oilseed, and root crops, wherein oilseed crops may include soybean, canola, oil seed rape, oil palm, sunflower, olive, corn, cottonseed, peanut, flaxseed, safflower, and coconut, among others.

III. Detection of Stress-Responsive or Photosynthetic Gene Expression

[0039] Expression levels of stress-responsive genes provided by the invention may be detected by various methods known to one of skill in the art. For example, expression levels of a gene in a tissue sample may be detected by isolating mRNA from the sample and quantitating mRNA transcripts by reverse transcription polymerase chain reaction (RT-PCR) using primers directed to the mRNA to be detected. Alternatively, RNA-sequencing or microarray technologies can be used to identify genome-wide gene expression changes, including all stress-responsive genes, at different times of day. The data can be analyzed to find optimal times of day to detect expression level differences in stress-responsive genes among parental strains.

[0040] Detection of stress-response gene expression according to methods of the present invention may be carried out at any time of day. In certain embodiments, stress-responsive gene expression levels may be detected in ACD6 at ZT18, in COR78 at ZT9, and in COR47 at ZT9. In general, the optimal time of day in which the stress-responsive gene expression differences between the parents are predictive of hybrid performance, which corresponds to the peak of gene expression for a given gene in the diurnal cycle. Therefore, to find optimal times of day for hybrid performance prediction, gene expression of stress-responsive genes should be assayed for at least 24 hours to identify the peak times of gene expression in the desired parental types.

IV. Selection and Breeding Methods

[0041] The present invention provides methods for identifying parent plants which can be crossed to produce a hybrid plant having a particular agronomic trait. Hybrid plants resulting from a cross according to the invention may exhibit an improved trait compared with either of the parent plants, or compared with the mid-parent value (MPV) for a trait of interest. In certain embodiments, the improved trait is increase biomass or increased yield.

[0042] One application of the present methods is therefore in breeding programs aimed at increasing the presence or degree of favorable traits in plants. This can be accomplished through a single round of breeding, or by repeated crossing of progeny plants with other suitable parent plants identified using the methods of the invention. Multiple rounds of crossing, including backcrossing, using the selection methods provided herein are contemplated by the invention.

EXAMPLES

[0043] The following Examples are illustrative of the invention described herein and should not be read to limit the scope of the invention to the particular aspects described herein. The methods described below were used in the applicable Examples unless described otherwise.

Plant materials and growth conditions

[0044] Plant materials include the following Arabidopsis thaliana ecotypes, which were used to generate Fl hybrids; C24, Columbia (Col), Cvi-0 (CS22614), Est-1 (CS22629), Ler, Nd-1 (CS22619), Oy-0 (CS22658), Sorbo (CS22653), Wei-0 (CS22622), and Ws. Crossing was carried out as previously described (Miller, et al. G3 (Bethesda) 2:505-513, 2012). An acd6- 1 hyperactive mutant (Max Planck Institute for Developmental Biology, Tubingen, Germany), and ccal-11 (CS9378), lhy21 (CS9379), and ccal-l llhy21 (CS9380) T-DNA insertion mutants in the Ws background (Arabidopsis Biological Resource Center) were also used. All plants were grown under a 16/8-h light/dark cycle with temperatures of 22 °C (light) and 20°C (dark) on soil, and rosette leaves from ~3 week old plants before flowering were collected for RNA analysis, unless otherwise noted. For qRT-PCR validation, plants were grown in three biological replicates, and leaves were harvested every three hours for two diurnal cycles (48 hours). For plant transformation, 4-5 week old plants were used for Agrobacterium tumefaciens- mediated transformation through floral dipping.

Biomass measurement

[0045] Biomass was measured after drying rosettes before bolting at 80°C for 24 hours as previously described (Miller, et al. G3 (Bethesda) 2:505-513, 2012).

Trypan blue staining

[0046] Trypan blue staining solution was prepared by adding 20 mg of Trypan blue to 40 ml of lactophenol solution for a final concentration of 0.5 mg rnl^-1. Leaves were incubated for 3 h in a sufficient amount of solution to immerse the tissue. The tissue was then cleared in a sufficient amount of chloral hydrate solution to cover the tissue (25 g of chloral hydrate per 10 ml water) for 30 min.

mRNA-seq library preparation

[0047] Total RNA was isolated from mature leaves (3 week-old A. thaliana plants) before bolting using plant RNA purification reagent (Invitrogen). The integrity of extracted RNA was analyzed by resolving 1 μg RNA in a 1%-formaldehyde denaturing agarose gel. An aliquot of 10 μg total RNA was used to prepare mRNA-seq libraries using Illumina mRNA- seq sample preparation kit (Illumina). Libraries with a range of 200+25 bp were excised and amplified for subsequent cluster generation and massively parallel sequencing using the Illumina Genome Analyzer Gil with read lengths of 85 bp.

mRNA-seq read mapping and expression quantification

[0048] mRNA-seq reads from 12 libraries (Col, C24, ColxC24 and C24xCol at three time points) were mapped to the TAIR9 genome and cDNA sequence using BFAST (Blat-like Fast Accurate Search Tool, publically available on the internet). Transcript levels were quantified by counting reads per kilobase per million mapped reads (RPKM). First, all unique reads without alternative splice isoforms were used to calculate a preliminary RPKM. Second, unique reads with isoform exons were weighted using the preliminary RPKM. Final RPKMs were calculated by combining the unique and weighted multiple mapped reads. Primary isoforms were used for further analysis. These isoforms could indicate a role for splicing variants of circadian clock genes in a temperature-dependent mechanism.

Bioinformatics analyses

[0049] Differentially-expressed genes were identified using the R-package DEGseq with a log2 fold change > 10.51 and a FDR corrected p-value (q-value) < 0.05 (Table 6). Heat maps were generated using Gene-E (Broad Institute, publically available on the internet), and clustering was performed using the default settings (one minus pearson correlation). RPKM values used to generate heat maps are located in Table 7, along with locations of EE and CBS motifs and circadian correlations of the genes displayed in the heat maps. GO analysis was performed and figures were generated using the agriGO website. The hypergeometric test was used to identify enriched (FDR adjusted p-value < 0.05) GO categories relative to the genome background. Genes were identified as biotic or abiotic stress-responsive by GO term analysis, followed by manual removal of uncharacterized genes to ensure that the classifications were accurate.

[0050] SNP data for C24 was obtained from the Arabidopsis 1001 Genomes database (available on the internet). Reads mapped to regions containing SNPs were extracted and then assigned to reads from Col or C24 according to the SNP database. The total RPKM value in hybrids was split to Col and C24 alleles based on the ratio between the number of reads mapped to either Col and C24. To calculate cis and trans effects, a previously published strategy was employed (Shi, et al. Nat. Commun. 3, 950, 2012). Log2-transformed ratios were used to estimate allelic expression divergence, such that gene-expression divergence (A) between ecotypes was calculated by log2(Col/C24) and the allelic ratio in the hybrids by log2(FlCol/FlC24). Genes which showed different expression between parents (log2 fold change > 111), but the same allelic expression within the hybrid (log2 fold change < 10.51), were considered to be caused by only trans effects. A gene was considered having only cis effects if the ratio of parental expression was equal to the allelic expression in hybrid or allotetraploid (A=B, log2 fold change < 10.51). [0051] Circadian-regulated genes were identified by comparing a given dataset (i.e. DEG or MPV gene lists in parents and hybrids, respectively) with the intersection of two different microarray experiments (Covington + Edwards intersection).

[0052] Integrative Genomics Viewer (IGV) was used to display publically available mRNA- seq, sRNA-seq, and methylation data (Shen, et al. Plant Cell 24, 875-892, 2012) (GSE34658). Genes were identified as having a higher methylation level than the MPV if average methylation differences were 0.1 or greater for CG and CHG contexts and 0.05 or greater for CHH contexts, similar to the criteria used by Shen, et al.

[0053] For QTL analysis, genes present in QTL intervals were tested for significant overlap with DEGs using the GeneSect Tool, which implements a non-parametric randomization test which can determine whether the overlap between two gene lists is higher or lower than expected by chance, from the Virtual Plant website, as well as the hypergeometric test in R.

RNA Extraction and qRT-PCR

[0054] Tissue collected for gene expression analysis was collected from plants before bolting (3 week old plants unless noted otherwise) at indicated Zeitgeber time (ZT0 = dawn). Total RNA was extracted using Concert Plant RNA Reagent (Invitrogen) and digested with RQ1 RNase-Free DNase (Promega) according to the manufacturer's instructions. cDNA was synthesized using ΙμΜ oligo dT (12-18) primer (GeneLink) from 1 μg DNase-treated RNA using the Omniscript RT Kit (Qiagen) according to the manufacturer's instructions. For qRT- PCR, FastStart Universal SYBR Green Master (Rox) (Roche Applied Science) was used for PCR in the presence of gene-specific primers and 2 μΐ_^ of diluted cDNA template. Expression levels of target genes were normalized against transcript levels of ACT7 (At5g09810) and relative expression levels of transcripts were calculated. The primer sequences are listed in Table 1.

"able 1. Oligonucleotide primers used for expression ana yses and cloning.

Name SEQ ID NO. Sequence (5' - 3') Description

ACT7-F 1 GTCTGTGACAATGGAACTGGAA ACT7 (At5g09810) qPCR

ACT7-R 2 CTTTCTGACCCATACCAACCAT ACT7 (At5g09810) qPCR

ACD6-F 3 ATCACTGCAATTGCCCATGT ACD6 (At4g 14400) qPCR

ACD6-R 4 ACACGCCACACAACCAAAA ACD6 (At4gl4400) qPCR

COR78-F 5 CTTGATGGTCAACGGAAGGT COR78 (At5g52310) qPCR

COR78-R 6 CAATCTCCGGTACTCCTCCA COR78 (At5g52310) qPCR

PR1-F 7 CGTTCACATAATTCCCACGA PR1 (At2gl4610) qPCR

PR1-R 8 AAGAGGCAACTGCAGACTCA PR1 (At2gl4610) qPCR

COR47-F 9 CGGTACCAGTGTCGGAGAGT COR47 (Atlg20440) qPCR

COR47-R 10 ACAGCTGGTGAATCCTCTGC COR47 (Atlg20440) qPCR

PR5-F 11 TCGAGGATTTTCAAGAACGC PR5 (Atlg75040) qPCR

PR5-R 12 AAGCTTCGGTTTTTAAGGGC PR5 (Atlg75040) qPCR

COR15A-F 13 GAAAAAAACAGTGAAACCGCAGAT COR15A (At2g42540) qPCR

COR15A-R 14 CCACATACGCCGCAGCTT COR15A (At2g42540) qPCR

HSPR02-F 15 GAGGAAGACAGAGTGCGATAAG HSPR02 (At2g40000) qPCR

HSPR02-R 16 CACTAACTGCCTATACCCAAAGA HSPR02 (At2g40000) qPCR

RD22-F 17 GATTCGTCTTCCTCTGATCTG RD22 (At5g25610) qPCR

RD22-R 18 TGGGTGTTAACGTTTACTCCG RD22 (At5g25610) qPCR

PCC1-F 19 ACAAATCTCACATCCTCACTCC PCC1 (At3g22231) qPCR

PCC1-R 20 GCCCTGATGAAGTCTCTGAAG PCC1 (At3g22231) qPCR

RD28-F 21 TTCGACGCAGAGGAGCTTACCA RD28 (At2g37180) qPCR

RD28-R 22 TACGAACTCGGCGATGACTGCT RD28 (At2g37180) qPCR

LHCB1.4-F 23 GCCTTCGCTACCAACTTCGTC LHCB1.4 (At2g34430) qPCR

LHCB1.4-R 24 AACCGGATACACACAACTCGATC LHCB1.4 (At2g34430) qPCR

PORB-F 25 GTGGACGGCAAGAAAACGTT PORB (At4g27440) qPCR

PORB-R 26 GGCTCCAGTGACCACCACAT PORB (At4g27440) qPCR

CAB2-F 27 ATTCGCAAGGAACCGTGAGCTAGA CAB2 (Atlg29920) qPCR

CAB2-R 28 TGAACCAGCCTTGAACCAAACTGC CAB2 (Atlg29920) qPCR

LHCB4.2-F 29 GCCGCCACTTCAACCGCCGCTGCTG LHCB4.2 (At3g08940) qPCR

LHCB4.2-R 30 CCCGTAGTCCCCGACAAGTGAACCG LHCB4.2 (At3g08940) qPCR

CCA1-F 31 CCTCGTCAGACACAGACTTCCA CCA1 (At2g46830) qPCR

CCA1-R 32 CCGCAGTAGAATCAGCTCCAATA CCA1 (At2g46830) qPCR

LHY-F 33 GGGACAAAGACTGCTGTTCAGAT LHY (Atlg01060) qPCR

LHY-R 34 TTTGTGAAGAACTTTTGTGCATGA LHY (Atlg01060) qPCR

TOC1-F 35 GTTGATGGATCGGGTTTCTC TOC1 (At5g61380) qPCR

TOC1-R 36 TCATGACCCCATGCATACAG TOC1 (At5g61380) qPCR

Xhol pCOR78-F 37 ccatCTCGAGAGATTTGGGGTTTTGCTTTTG COR78 promoter cloning

Ncol pCOR78-R 38 ccatCCATGGGAGTAAAACAGAGGAGGGT COR78 promoter cloning

Xhol pACD6-F 39 ccatCTCGAGAAGAGTTTGTAGCCTATTCAAAG ACD6 promoter cloning

Ncol pACD6-R 40 ccatCCATGGGGTTATCGAGAGGAGTGGTGGTC ACD6 promoter cloning

RsrII COR78-F 41 ccatCGGACCGATCAAACAGAGGAACCACC COR78 transcribed region cloning

Aatll COR78-R 42 ccatGACGTCTTAAAGCTCCTTCTGCACCGG COR78 transcribed region cloning amiCor78-l 43 gaTATAGGTAACTTCGTTGTCACctctcttttgtattcca For amiRNA against COR78 amiCor78-2 44 agGTGACAACGAAGTTACCTATAtcaaagagaatcaatga For amiRNA against COR78 amiCor78-3 45 agGTAACAACGAAGTAACCTATTtcacaggtcgtgatatg For amiRNA against COR78 amiCor78^ 46 gaAATAGGTTACTTCGTTGTTACctacatatatattccta For amiRNA against COR78

Aatll COR78 A 47 ccatGACGTCCTGCAAGGCGATTAAGTTGGGTAAC For final amiRNA sequence

Xmal COR78 B 48 ccatCCCGGGGCGGATAACAATTTCACACAGGAAACAG For final amiRNA sequence amiACD6-l 49 gaTTAATGGTGACTAAAGGCCGTctctcttttgtattcca For amiRNA against ACD6 amiACD6-2 50 agACGGCCTTTAGTCACCATTAAtcaaagagaatcaatga For amiRNA against ACD6 amiACD6-3 51 agACAGCCTTTAGTCTCCATTATtcacaggtcgtgatatg For amiRNA against ACD6 amiACD6-4 52 gaATAATGGAGACTAAAGGCTGTctacatatatattccta For amiRNA against ACD6

RsrII ACD6 A 53 ccatCGGACCGCTGCAAGGCGATTAAGTTGGGTAAC For final amiRNA sequence

Aatll ACD6 B 54 ccatGACGTCGCGGATAACAATTTCACACAGGAAACAG For final amiRNA sequence

Note: Restriction enzyme sites are underlined and capitalized letters correspond to the miRNA and miRNA ^: sequences.

Plasmid constructs [0055] For luciferase reporter constructs, genomic DNA from Col, C24, and Ws was used to amplify ACD6 and COR78 promoter regions. SNPs between Col and C24 promoter and coding regions were identified using Polymorph (Ossowski, et al , publically available on the internet). The amplified fragments were cloned into pGEM-T vector (Promega) for sequence verification. The Promoter :LUC plasmid constructs were generated by inserting luciferase gene between the restriction enzyme sites Ncol and BamHI in the pFAMIR plasmid (Yadegari, University of Arizona). For 35S-driven overexpression constructs, the transcribed region of COR78 from Col or C24 was cloned into the pF35SE vector (A vector for 35S- driven gene expression, Yadegari, University of Arizona) between RsrII and Aatll restriction sites. Artificial miRNAs were designed using the WMD3 web app (Ossowski and Fitz, publically available on the internet) against a conserved region in Col and C24 and then amplified using the pRS300 vector as a template. The amplified fragments were cloned into pGEM-T vector (Promega) for sequence verification. The artificial miRNAs were then cloned into the pF35SE vector between RsrII and Aatll restriction sites for amiACD6 and between Xmal and Aatll restriction sites for amiCOR78. All constructs were individually cloned into Agrobacterium strain GV3101 for plant transformation and seeds were screened on 1 % (w/v) agar with Murashige and Skoog (MS) media containing 7.5 mg/L phosphinothricin. The primer sequences are listed in Table 1.

Luciferase assays

[0056] Plants containing either ACD6:LUC or COR78:LUC constructs were analyzed using a Packard TopCount luminometer as previously described. Seeds were sterilized with bleach and 75% ethanol and plated on 1 % (w/v) agar with MS media containing 7.5 mg/L phosphinothricin. Seeds were stratified 2 days in the dark at 4°C and then transferred into 16- h light and 8-h dark cycles for 7 days, and then transferred to MS containing no selection for 3 days. Seedlings were transferred to white microtiter plates (Nunc, Denmark) containing agar MS medium plus 30g sucrose/L and 30 iL of 0.5 mM luciferin (Gold Biotechnology). Microtiter plates were covered with clear plastic MicroAmp sealing film (Applied Biosystems, Foster City, CA) in which holes were placed above each well for seedling gas exchange. One day after addition of luciferin, plates were moved to the TopCount and interleaved with two clear plates to allow light diffusion to the seedlings. All luciferase data were analyzed using the Biological Rhythm Analysis Software System (BRASS, publically available on the internet). All period estimates were performed on rhythms from 24-120 hours using fast Fourier Transform-nonlinear least squares (FFT-NLLS) analysis.

Regression analysis

[0057] Regression analysis was performed using JMP 10 (JMP®, Version 10. SAS Institute Inc., Cary, NC, 1989-2007) with the default parameters and all graphs were generated using JMP. Data used for the regression analysis is in Table 4, and detailed results can be found in Table 8.

Cold- and SA-treatment experiments

[0058] The scheme for stress experiments is shown in Fig. 14. For cold-induction of gene expression (cold-shock), 6 two week old seedlings (3 seedlings were used per replicate) were removed from agar media and were placed into 5 ml culture tubes containing room- temperature liquid MS media with 3% sucrose 24 hours before cold- treatment. Cold- treatment was performed by placing the tubes in ice or leaving at 22°C for the control. After 60 minutes, tubes containing seedlings were placed back into 22°C and then whole seedlings (excluding roots) were snap frozen in liquid nitrogen at the ZT times listed in Fig. 14.

[0059] For SA-induction of gene expression, two-week old seedlings growing on agar media were sprayed with 1 mM SA (Sigma-Aldrich). After 60 minutes, whole seedlings (excluding roots) were snap frozen in liquid nitrogen for the first time point, and then at the subsequent ZT times listed in Fig. 14.

[0060] For longer-term cold stress application, two-week old seedlings growing on agar media were placed into a 4°C growth chamber of the same light intensity as control seedlings which were left at 22°C. Every 2-3 days, seedlings were photographed and rosette diameter was quantified using ImageJ. Every 2-3 days, seedlings were photographed and rosette diameter was quantified using ImageJ. After two weeks, seedlings were snap frozen in liquid nitrogen at ZT0, ZT9, and ZT15. A batch of seedlings was transferred to soil, placed at 22°C to allow for recovery, and rosette diameter was measured every 2-3 days. After one week biomass was measured.

[0061] For longer-term SA-treatment, two-week old seedlings growing on agar media were sprayed with lmM SA or water (control). Rosette diameter was measured every 2-3 days as described above. After one week seedlings were transferred to soil, sprayed with 1 mM SA again, and rosette diameter was measured every 2-3 days. After one more week, seedlings were snap frozen in liquid nitrogen at ZTO, ZT9, and ZT15 and biomass was measured.

[0062] To calculate relative expression ratios (R.E.R), expression levels of target genes were first normalized against transcript levels of ACT7 and then the induced value was divided by the control value (indicated by a dashed line in the figures). Data of absolute values are available but not shown owing to space limitations.

[0063] Relative growth rate (RGR) was calculated according to RGR = (lnD2 - lnDl)/t2 - tl, where Dl and D2 are rosette diameters at time points tl and t2, respectively.

Example 1 : Diurnal repression of stress-responsive genes in hybrids

[0064] To assess the role of diurnal stress-responsive gene expression in heterosis, we deep- sequenced mRNAs from 3-week old seedling leaves of highly heterotic Fl hybrids between A. thaliana Col and C24 ecotypes at zeitgeber time (ZT) 0 (dawn), ZT6 (midday), and ZT15 (dusk) (Fig. la). All experiments were performed in diurnal conditions where heterosis naturally occurs.

[0065] The percentage of differentially expressed genes (DEGs) between Fl hybrids and the mid-parent value (MPV) was highest (-5%) at ZT6 (noon), followed by -3% at ZTO (dawn), and -1.3% at ZT15 (dusk) (Table 6). Gene Ontology (GO) analysis (Fig. 8a-f) revealed that both photosynthetic and stress-responsive genes were enriched in the DEGs relative to all expressed genes (DEGseq q-value (FDR-adjusted p-value) < 0.05, see Methods). Thirty of 267-328 (10%) genes that were upregulated in reciprocal hybrids at ZT6 belonged to the photosynthetic processes using GO term analysis (Fig. 8g-k), consistent with previous observations of increased photosynthetic capacity in hybrids. Note that gene expression patterns were slightly and consistently different between the reciprocal Fl crosses (also below), which is known to be due to parent-of-origin effects on circadian rhythms and biomass heterosis. Among down-regulated genes, 3-13% were in the GO classifications of biotic and abiotic stress-responsive genes (Fig. le-j and Table 7). The repression of these genes, including 26 known biotic and 38 known abiotic stress-responsive genes (classified using GO term analysis, see Methods), occurred at different times of the day (Fig. lb,d). At ZT6, 2-3-fold more abiotic than biotic stress-responsive genes were repressed (Fig. lg,h). This trend was reversed at ZTO and ZT15, and biotic stress-responsive genes comprised a larger proportion of the repressed genes (Fig. le,f,i,j). The timing of repression in the hybrids was confirmed by quantitative Reverse Transcriptase-Polymerase Chain Reaction (qRT-PCR) analysis in two diurnal cycles (48 hours) in 3 biological replicates. Biotic stress-responsive genes such as ACCELERATED CELL DEATH6 (ACD6, At4gl4400), PATHOGEN AND ORCADIAN CONTROLLED 1 (PCC1, At3g22231), and HOMOLOG of SUGAR BEET HSl PRO-2 (HSPR02, At2g40000) were primarily repressed to below MPV levels between ZTO and ZT6 (Fig. 2a and Fig. 9a,b), and PATHOGENESIS-RELATED GENES 1 (PR1, At2gl4610) and PR5 (Atlg75040) were repressed at all times (Fig. 9c,d). However, cold stress-responsive genes, including COLD -REGULATED78 (COR78, At5g52310), COR47 (Atlg20440), COR15A (At2g42540), RESPONSIVE-TO-DESICCATION22 (RD22, At5g25610), and RD28 (At2g37180), were repressed in the middle and later parts of the day (Fig. 2b and Fig. 9e-h). A slight shift of the expression peaks between ecotypes may reflect their adaptation to fluctuating local environments. Moreover, expression rhythms of many stress-responsive genes are modulated by other signaling factors in addition to the circadian clock.

[0066] This genome-wide repression of stress-responsive genes could be caused by cis- and trans-acting factors in the hybrids, which was tested using all expressed genes. Consistent with the findings in the interspecific hybrids of Arabidopsis 32 or yeast 33, >43% more genes exhibited cis effects than trans effects in the Fl hybrids at ZTO, ZT6, and ZT15 (Fig. 10a). However, amongst stress-responsive genes, significantly more genes displayed irans-effects than ds-effects at all time points (FDR adjusted p<0.05, hypergeometric test, Fig. 10b). The irans-regulated genes of either parental origin could be repressed to the low-parent level or lower (Fig.10c -h), including several known biotic (Fig. lOi-1) and abiotic (Fig. lOm-p) stress- responsive genes. The genome-wide data suggest a role for trans-acting factors in the diurnal repression of stress-responsive genes in these hybrids.

[0067] These trans-acting factors are predicted to involve circadian-clock regulators, as the clock is known to regulate stress-responsive gene expression. Indeed, DEGs in the hybrids included circadian-regulated genes (Table 2), which were significantly enriched with the evening element (p=0.018) and CIRCADIAN CLOCK ASSOCIATED1 (CCA1, At2g46830)-binding site (p<10) (Table 7). The circadian clock regulators, CCA1 and LATE ELONGATED HYPOCOTYL (LHY, Atlg01060), were repressed in the middle of the day and upregulated around dawn (Fig. l la,b), and their feedback regulator TOC1 (At5g61380) showed the opposite expression changes (Fig. 11c), consistent with previous results. As a result of the clock regulation, the phases of up- and down-regulated DEGs in the hybrids at ZTO and ZT6 were clustered in the middle of the day and towards dawn, respectively (Fig. l ld-i). Hence, the expression of many circadian clock-associated genes and stress-responsive genes is coordinately changed, which promotes growth vigor in the hybrids.

Table 2. Percentage of DEGs in hybrids, which are circadian-regulated

Genotype_Time % circadian regulated p-value

MPV ZTO 16.17% N/A

Col x C24_ZT0 33.62% 7.21E-13

C24 x Col_ZT0 27.54% 8.88E-06

MPV_ZT6 16.08% N/A

Col x C24_ZT6 45.97% < 2.2e-16

C24 x Col_ZT6 36.53% < 2.2e-16

MPV_ZT15 16.64% N/A

Col x C24_ZT15 30.65% 2.83E-05

C24 x Col_ZT15 30.00% 0.0003352

Note: p-values were calculated using chi-square test of independence.

Example 2: Circadian rhythms and stress responses in Arabidopsis

[0068] Expression level changes in certain stress-responsive genes among different ecotypes may result from plants experiencing different stresses during the day and night, leading to natural variation. To determine natural variation of stress-responsive gene expression between ecotypes, we used two well-characterized biotic (ACD6) and abiotic (COR78) stress-responsive genes as examples in stable transgenic plants expressing a promoter:luciferase (LUC) reporter. ACD6:LUC and COR78:LUC displayed rhythmic activities in both Col and C24 ecotypes (Fig. 2c ,e) and showed natural variation in trans gene activity between Col and C24, consistent with the expression of endogenous genes (Fig. 2a,b). ACD6(C24):LUC in C24 was expressed at higher levels than ACD6(Col):LUC in Col (Fig. 2c). Likewise, COR78(Col):LUC in Col was expressed at higher levels than COR78(C24):LUC in C24 (Fig. 2e). Notably, the expression differences between the ecotypes were amplified when they were expressed in reciprocal combinations. For instance, ACD6(Col):LUC expression amplitudes in C24 were 10- 15 -fold higher than ACD6(C24):LUC levels in Col (Fig. 2d). COR78(C24):LUC amplitudes in Col were 7-8 fold higher than COR78(Col):LUC in C24 (Fig. 2f). Thus, genetic backgrounds could act as transacting factors to mediate rhythmic expression peaks of these stress-responsive genes, probably through altered binding of regulatory factors such as the clock proteins or other upstream regulators to the promoters between the ecotypes (Table 3).

Table 3. SNPs between Col and C24 ecotypes in ACD6 and COR78

ACD6 promoter

Position (bp) Col C24

8292298 T C

8292361 G A

8293280 A G

8293767 C A

8293798 C T

8293903 A C

8294012 C T

ACD6 coding region

Position (bp) Col C24

8296397 A G

8296493 A G

8296559 A G

8296617 T C

8296646 G A

8296650 T G

8296709 G A

8296909 A G

8296951 A G

8297194 C G

8297414 A T

8297500 G T

8297531 A G

8297960 T C

8298037 T C

8298049 T G

8298255 T G

COR78 promoter

N/A No SNPs

COR78 coding region

Position (bp) Col C24

21258687 G T

Note: SNP data was obtained from the Arabidopsis 1001 genomes database (hitp://^" i001 genomes. org/).

[0069] Tests were conducted to determine whether stress conditions affect rhythmic expression and if the clock regulates expression of stress-responsive genes in the clock mutants as in the wild-type. Since the clock mutants, namely, ccal-1, lhy21, and ccal-1 llhy21, are in the Ws ecotype background, Ws was used as the wild-type for comparison to the clock mutants. Salicylic acid (SA), a plant hormone that triggers biotic stress responses, induced elevated arrhythmic expression of ACD6(Ws):LUC in the transgenic plants (Fig. 3a). Likewise, cold-shock transiently induced COR78(Ws):LUC expression, but the induction was rhythmic and remained for -36 hours (Fig. 3b). In the absence of stress, the period of ACD6(Ws):LUC activity (24.26+0.30 s.e.m., 20 n=16 individuals per transgenic line) was nearly normal in the ccal-11 or lhy21 mutant, but was shortened by ~1 hour in the ccal- llhy21 double mutant (23.17+0.41, p<0.05) (Fig. 3c). However, both the expression amplitudes and periods of COR78(Ws):LUC were severely altered in the single (ccal-11, 23.73+0.51 ; lhy21 24.14+0.30, p < 0.05) or double (22.61+0.66, p < 0.05) mutant (Fig. 3e). Under stress conditions, SA-mediated induction of ACD6(Ws):LUC was hindered in the double mutant, but not in the single mutants (Fig. 3d). Induction of COR78(Ws):LUC after cold-shock was compromised in all mutants tested (Fig. 3f). These data indicate that the clock regulators, CCAl and LHY together, or CCAl alone, mediate expression amplitudes and periods of these biotic and abiotic stress-responsive genes in stress and non-stress conditions, as previously suggested.

Example 3: Stress-responsive expression as a predictor for heterosis

[0070] The diurnal regulation of stress-responsive genes could provide a basis for natural variation among diverse ecotypes tested (Fig. 4a,b, Table 4, and Fig. 12a-d), which is consistent with their wide-geographical distributions. For example, in C24 (Coimbra, Portugal) and Cvi (Cape Verde Islands), abiotic genes were poorly expressed (Fig. 4a,b and Fig. 12c,d), but biotic genes were highly expressed at ZT18, which correlated with higher SA levels and more necrotic lesions on mature leaves in C24 than in Col 15 (Fig. lc). This is because these ecotypes are adapted to warmer environments with relatively more pathogens 40. However, in Col (Columbia, USA), Ler (Germany), and Ws (Russia), where the plants are presumably adapted to colder climates, COR78 and COR47 was highly expressed at ZT9 (Fig. 4b and Fig. 12d), but ACD6 and PR1 were poorly expressed (Fig. 4a and Fig. 12c). Interestingly, no ecotype tested showed low expression levels of both biotic and abiotic stress-responsive genes, indicating that the basal level of stress responses reflects a genetic basis for a given ecotype's adaptation to its local environment. Thus, the overall repression of stress-responsive genes of both parental origins is unique in the hybrids, which could affect heterosis. This also suggests that natural variation in stress responsive-gene expression between parents could be used to predict the level of heterosis. Table 4. Biomass and gene expression values used for regression analysis

are relative to ACT7 and are an average of three biological replicates. The rows containing the parental gene expression ratio shows the absolute value of the log2 fold change in expression level between parents. [0071] To test the relationship between natural variation of stress responses and heterosis in hybrids, a regression analysis was performed between the mean increase in biomass relative to the mid-parent value as the dependent variable and the expression level difference for a given stress-responsive gene between the parents at different time points as the independent predicting variable (Fig. 4c-e, Table 4, and Fig. 12e-p). It was determined that expression differences of the stress-responsive genes between parental ecotypes at certain times of day, including ACD6 at ZT18, COR78 at ZT9, and COR47 at ZT9 (Fig. 4c-e), were significantly correlated with biomass heterosis (p<0.05, Table 8). This indicates that the timing of measurement of expression in the parents is relevant to predicting the level of heterosis based on expression differences of stress-responsive genes. Using data from an independent study, quantitative trait loci (QTL) for biomass heterosis were compared with the DEGs in the hybrids (Table 5). The DEGs overlapped significantly with two QTL regions using both hypergeometric and non-parametric randomization tests (p=0.01). Moreover, many stress- responsive genes including COR15A and PR2, both of which were down-regulated in the hybrids, coincided within the QTL regions, suggesting a potential role of these candidate genes underlying the QTL of biomass heterosis.

Table 5. Genes in common between biomass quantitative trait loci (QTLs) and differentially expressed genes (DEGs)

Note: The table shows the overlap between all DEGs in hybrids (982 total) and QTL regions for dry weight at day 15 (DW15) from Meyer et al 2010. QTL regions were identified in the RILs and verified in the ILs (See Supplementary Table 5 in Meyer et al 2010).

[0072] According to the hypothesis that genetic distance plays a role in heterosis, higher levels of heterosis could be obtained from the parents with larger expression differences in stress-responsive genes. For example, the hybrids between Col and C24 with larger COR78 and ACD6 expression differences (Fig. 2) displayed higher heterosis (Fig. la). Using this concept, two additional high-vigor Fl hybrids (C24XLer and C24XWs) were predicted (Fig. 5a-d), which correlated with larger ACD6 and COR78 expression differences between the parents (C24 vs. Ler and C24 vs. Ws) (Fig. 4a,b). In contrast, if the parents had similar levels of ACD6 and COR78 expression (for example, Col vs. Ws and Col vs. Ler) (Fig. 13a-d), their Fl hybrids displayed relatively low levels of heterosis (ColXWs and ColXLer) (Fig. 5e,f and Fig. 12e,f). Moreover, in the high-vigor hybrids, both COR78 and ACD6 were repressed at most time points (Fig. 12g-j), whereas in low-vigor hybrids, COR78 and ACD6 were generally repressed but also upregulated at several time points (Fig. 12a-d). Another Fl hybrid (EstXCol) displayed high biomass heterosis (Fig. 12k), which correlated with repression of both ACD6 and COR78 (Fig. 121,m). Example 4: Hybrids have a timed stress-response strategy

[0073] It is paradoxical that in the absence of stress, stress-responsive genes were repressed in the hybrids, and yet hybrids are generally more tolerant than parents to stresses. To address this paradox, growth vigor and stress responses were tested in the hybrids and their parents during short- and long-term stress treatments. After short-term cold shock treatment at ZTO or ZT15 (see Methods and Fig. 14), both COR78 and COR15A transcripts were transiently induced in ColXC24 hybrids and their parents (Fig. 15a-d). Cold-treatment caused a greater increase in gene expression at ZT15 than at ZTO (Fig. 15, a vs. b and c vs. d) due to gating of induction by the circadian clock and diurnal regulation of stress responses. Interestingly, higher than MPV induction of stress-responsive genes in the hybrids occurred only at some specific time points (e.g., COR15A at ZT3 and ZT9 and COR78 at ZT9). Similarly, after SA- treatment, both ACD6 and PR1 were also expressed at higher levels at ZT15 than at ZTO (Fig. 15e-h). In the hybrids, induction of PR1 and ACD6 was delayed for a few hours after SA treatment, but ultimately reached a level higher than the MPV at several time points.

[0074] Under long-term stress (cold or SA) conditions (see Methods and Fig. 14), hybrids maintained higher relative growth rates (RGR) than their parents (Fig. 4f-i). After removal from stress conditions the RGR of hybrids accelerated more than that of the parents, consistent with increased protection from freezing damage during cold stress. As a result, hybrids accumulated more biomass than parents (Fig. 15i,j). Hybrids also showed higher than MPV induction of cold-responsive genes at certain times after two weeks growing in the cold, although this trend was less obvious after the long-term treatment with SA (Fig. 15k-n). These data collectively suggest that stress-responsive genes, which are normally repressed in the hybrids, can be rapidly induced at certain time points under the stress, which we propose may preserve energy and metabolism to promote growth in the presence of stress. This is reminiscent of the trade-off between growth and fitness in diploids.

Example 5: Effects of stress-responsive genes on biomass

[0075] It is well established that constitutive defense responses decrease biomass, growth rate, and seed production, and R-genes reduce fitness in the absence of pathogens. To determine if the repression of stress-responsive genes in the hybrids could promote growth vigor, the effects of repressing and overexpressing ACD6 and COR78 on biomass in Col and C24 ecotypes was investigated (Fig. 5g-m). The dominant negative mutant acd6-l accumulated less biomass than the wild-type due to the production of higher amounts of SA and spontaneous cell death (Fig. 5g). Conversely, knockdown of ACD6 (amiACD6) using artificial microRNAs (amiRNA) showed a modest but non-significant increase in biomass relative to Col (Fig. 5h), whereas amiACD6 in C24 showed significant increases in biomass (Fig. 5i). This is probably because the basal expression level of ACD6 is low in Col, and SA levels are naturally elevated in C24. Overexpressing COR78 in either Col or C24 caused biomass reductions at statistically significant levels (Fig. 51,m), and repressing COR78 increased biomass in Col but not in C24 (Fig. 5j,k). This is likely because the basal expression level of COR78 is low in C24, and expression levels of cold stress-responsive genes are high in Col. Thus, repressing COR78 has a smaller effect in C24 than in Col. Biomass increase or reduction in individual transgenic lines was anti-correlated with expression levels of ACD6 and COR78, respectively (Fig. 5n-p). Thus, reducing or increasing expression of a single stress-responsive gene in diploids can alter biomass, and simultaneous suppression of many of these genes in hybrids could lead to biomass heterosis.

Example 6: Epigenetic reprogramming of hybrids

[0076] The available data indicate that natural variation and circadian regulation of stress- responsive gene expression is correlated with heterosis. Different ecotypes experience diurnal and seasonal changes in their corresponding environments, leading to differences in circadian regulation of stress-responsive gene expression. The hypothesis was tested that, over time, "long-term" adaptation to the local environment results in genetic and epigenetic variation between parents, which is reprogrammed in hybrids to change the expression of circadian regulators and stress-responsive genes, and possibly metabolites, leading to heterosis. Consistent with the role of epigenetic variation in heterosis, the overall levels of CG, CHG and CHH (H = A, T, or C) methylation in many genes, including biotic and abiotic stress- responsive genes (Fig. 6a,b), were generally increased in hybrids compared to parents (Fig. 6c-k). These results are reminiscent of dynamic changes in transposon methylation that can regulate the expression of neighboring genes in response to biotic stress and of changes in CHH methylation that mediate circadian gene expression and heterosis in reciprocal Fl hybrids. In DNA methylation mutants, the circadian-clock genes, CCA1 and LHY, and stress-responsive genes, including ACD6 and PRl, are upregulated, indicating repression of these genes by DNA methylation. Consequently, increased methylation levels in hybrids selectively repress stress-responsive genes. [0077] On the basis of these findings, a general role is proposed for the repression of stress- responsive genes by both epigenetic mechanisms and circadian clock regulators in hybrids to promote biomass heterosis under non-stress conditions. Under stress conditions, hybrids induce expression of repressed stress-responsive genes at certain times of the day to combat the effects of stress and recover faster than their parents after stress conditions are removed. This role for the timing of stress responses in biomass heterosis is reminiscent of the connection between altered clock gene expression and growth vigor in Arabidopsis allopolyploids and diploids. In particular, repressing CCA1 expression peaks in TOCl: :ccal(RNAi) transgenic plants during the day increases starch content and biomass, while overexpressing T0C1 ::CCA1 in the transgenic plants decreases starch content and biomass. These findings provide a direct link between circadian regulators and biomass heterosis through promoting the clock output pathways of photosynthesis and starch metabolism. These data suggests that this clock-heterosis link involves another clock output pathway, namely, stress-responses. In addition to regulation by the circadian clock, stress responsive genes are epigenetically regulated in the hybrids.

[0078] Balancing stress-response to promote heterosis can explain heterosis as well as hybrid necrosis (Fig. 7). Hybrid necrosis, as previously reported, was likely caused by the induction of stress-responsive genes. Indeed, expression of several stress-responsive genes including PR1, PR2, and PRS is elevated in these necrotic hybrids. However, the higher- vigor hybrids have a better-timed stress-response strategy whereby stress-responsive genes are generally repressed under non-stress conditions and selectively induced at certain times under the stress, thus balancing the tradeoff between a rapid requirement for stress responses and long- term maintenance of growth vigor. This stress-response strategy is inherited from adaptively divergent ecotypes, with more competitive species having lower levels of constitutive expression of stress-responsive genes but higher levels of inducible resistance. Hybrids may simply have exploited this adaptive response. Hybrid plants recovered faster than their parents after encountering stresses, suggesting that hybrids have a higher potential to promote growth by regulating stress responses under both stress and non-stress conditions. Using stress responsive genes in several time points, the biomass heterosis based was accurately predicted by their expression levels between different pairs of parental lines. This link between natural variation, circadian regulation of stress-responsive genes and heterosis provides a method of selecting parents to improve hybrid production of plants and animals. Table 6. List of differentially expressed genes (DEGs) in hybrids between C24 and Col at ZTO, ZT6, and ZT15

171.3832

AT5G55450.1 46.4321 1.884031342 8.864736 7.67E-19 4.28862E-16 4.28862E-16

1

AT5G08760.1 156.9006 39.3351 1.995961713 8.805219 1.31E-18 7.15752E-16 7.15752E-16

193.6132

AT1G72930.1 59.82 1.694477872 8.772977 1.74E-18 9.34944E-16 9.34944E-16

5

ATCGOl 180.1 953.6365 624.501 0.610735573 8.738779 2.36E-18 1.2418E-15 1.2418E-15

AT3G63160.1 834.9635 1251.65 -8.675992 4.10E-18 2.11966E-15 2.11966E-15

0.584046159

AT5G17920.1 643.7895 1012.39 -0.65310421 -8.671221 4.28E-18 2.16947E-15 2.16947E-15

AT5G13930.1 272.441 521.067 -8.609819 7.32E-18 3.64588E-15 3.64588E-15

0.935525057

AT2G38540.1 721.902 1107.59 -8.604886 7.64E-18 3.73818E-15 3.73818E-15

0.617549027

AT1G75040.1 134.8691 30.3642 2.151116584 8.555388 1.17E-17 5.64758E-15 5.64758E-15

114.5984

AT5G44568.1 20.9744 2.4498864 8.51371 1.68E-17 7.82405E-15 7.82405E-15

75

151.0934

AT5G49360.1 39.4663 1.936747943 8.474792 2.36E-17 1.07557E-14 1.07557E-14

5

68.99072

AT1G65960.1 0 7.385301513 8.396417 4.60E-17 2.03449E-14 2.03449E-14

5

AT1G64770.2 63.37355 0 6.599604979 8.244165 1.66E-16 7.23429E-14 7.23429E-14

AT3G16240.1 897.3015 596.715 0.58855078 8.214911 2.12E-16 9.09099E-14 9.09099E-14

AT1G21250.1 100.4144 17.3973 2.529030848 8.111644 4.99E-16 2.04252E-13 2.04252E-13

AT1G44575.1 796.1525 1171.49 -8.023658 1.03E-15 4.01815E-13 4.01815E-13

0.557227934

141.5520

AT5G54610.1 38.9761 1.860670998 7.990298 1.35E-15 5.19549E-13 5.19549E-13

5

AT2G 18660.1 70.84787 6.21963 3.509823817 7.951095 1.85E-15 6.93975E-13 6.93975E-13

AT5G54270.1 788.598 1147.09 -7.711238 1.25E-14 4.55267E-12 4.55267E-12

0.540616632

104.5118

AT1G15690.2 251.059 -7.696117 1.40E-14 5.05743E-12 5.05743E-12

65 1.264359706

AT5G24530.1 87.90475 14.8102 2.569350001 7.655411 1.93E-14 6.76979E-12 6.76979E-12

AT1G72930.2 148.6832 46.4299 1.679115565 7.63969 2.18E-14 7.55302E-12 7.55302E-12

52.04808

AT2G24790.2 0 6.232964678 7.521122 5.43E-14 1.86026E-11 1.86026E-11

5

AT5G59890.2 0 52.3849 -7.483181 7.25E-14 2.45425E-11 2.45425E-11

6.072965409

AT1G56120.1 57.44258 3.82696 3.907849822 7.433484 1.06E-13 3.53478E-11 3.53478E-11

AT3G29240.2 287.539 140.079 1.037516958 7.418725 1.18E-13 3.90421E-11 3.9042 IE- 11

AT1G09310.1 563.933 853.757 -7.330523 2.29E-13 7.47895E-11 7.47895E-11

0.598301734

AT3G29240.1 281.814 138.41 1.025795104 7.278667 3.37E-13 1.07425E-10 1.07425E-10

AT1G04040.1 258.055 456.789 -7.204214 5.84E-13 1.83882E-10 1.83882E-10

0.823849325

AT5G45775.1 48.698 0 6.821822772 7.18689 6.63E-13 2.06406E-10 2.06406E-10

AT3G17390.1 252.017 445.662 -7.104898 1.20E-12 3.66627E-10 3.66627E-10

0.822428898

AT3G16370.1 343.0555 559.223 -6.917854 4.59E-12 1.35111E-09 1.35111E-09

0.704981701

AT3G26060.1 496.6235 752.205 -6.887753 5.67E-12 1.65235E-09 1.65235E-09

0.598973365

AT4G 14400.1 87.08665 19.8483 2.133436131 6.839911 7.92E-12 2.28576E-09 2.28576E-09

AT3G23810.1 159.5375 310.494 -6.800591 1.04E-11 2.9741E-09 2.9741E-09

0.960669814

48.83483

AT1G57720.2 3.78518 3.68947681 6.7236 1.77E-11 4.95738E-09 4.95738E-09

5

AT1G22930.2 40.75225 0 5.779820388 6.677983 2.42E-11 6.63847E-09 6.63847E-09

AT3G11945.2 0 41.6028 -5.64437038 -6.673383 2.50E-11 6.78216E-09 6.78216E-09 44.51555

AT1G13470.1 2.72981 4.027437065 6.604207 4.00E-11 1.06326E-08 1.06326E-08

4

582.4196

AT1G31580.1 843.076 -6.526891 6.71E-11 1.75245E-08 1.75245E-08

45 0.533603672

ATCG00020.1 80.63565 18.8952 2.093398024 6.504905 7.77E-11 2.00979E-08 2.00979E-08

AT5G37260.1 513.717 334.83 0.617545021 6.472945 9.61E-11 2.43863E-08 2.43863E-08

AT4G 14400.3 78.4433 18.0911 2.116370098 6.459482 1.05E-10 2.64129E-08 2.64129E-08

AT2G05070.1 380.147 590.189 -6.443349 1.17E-10 2.91134E-08 2.91134E-08

0.634619626

AT1G66970.1 396.5445 243.271 0.704918425 6.356111 2.07E-10 5.10846E-08 5.10846E-08

AT4G 14400.2 74.82275 17.3254 2.11058833 6.297965 3.02E-10 7.24925E-08 7.24925E-08

80.03337

AT1G72910.1 20.1556 1.989421018 6.275407 3.49E-10 8.30957E-08 8.30957E-08

5

AT1G04430.2 35.78905 0 5.613820815 6.256759 3.93E-10 9.28539E-08 9.28539E-08

AT5G20630.1 350.177 545.662 -6.24406 4.26E-10 9.98584E-08 9.98584E-08

0.639923249

AT3G60420.1 61.95607 11.4365 2.437600033 6.242145 4.32E-10 1.00233E-07 1.00233E-07

AT1G19960.1 80.94655 20.9954 1.946896319 6.223958 4.85E-10 1.11631E-07 1.11631E-07

AT1G12090.1 81.92595 184.516 -1.17135352 -6.199355 5.67E-10 1.29468E-07 1.29468E-07

34.66080

AT5G40890.2 0 5.167025653 6.126398 8.99E-10 1.98651E-07 1.98651E-07

5

39.91199

AT2G05540.1 2.93739 3.764215662 6.120709 9.32E-10 2.04228E-07 2.04228E-07

5

AT3G47370.3 0 34.5368 -6.0692 1.29E-09 2.79574E-07 2.79574E-07

6.193382566

AT4G13510.1 86.51895 25.2621 1.776041584 6.055394 1.40E-09 3.0224E-07 3.0224E-07

AT5G03350.1 60.1157 11.6823 2.36341748 6.041106 1.53E-09 3.27682E-07 3.27682E-07

AT5G42100.2 34.4656 1.48951 4.532247416 5.98665 2.14E-09 4.55031E-07 4.55031E-07

AT1G67870.1 567.521 394.117 0.526049797 5.936905 2.90E-09 6.12254E-07 6.12254E-07

AT2G40100.1 199.685 341.719 -5.897282 3.70E-09 7.7301E-07 7.7301E-07

0.775084502

AT1G29070.1 494.5855 710.446 -5.870197 4.35E-09 8.96835E-07 8.96835E-07

0.522505052

AT2G44120.2 8.63801 52.7006 -5.837352 5.30E-09 1.08461E-06 1.08461E-06

2.609048495

30.95762

AT3G17510.2 0 5.877117413 5.818922 5.92E-09 1.1847E-06 1.1847E-06

35

22.99125

AT3G09820.2 80.6454 -5.724347 1.04E-08 2.047E-06 2.047E-06

5 1.810507026

162.4307

AT1G55330.1 287.074 -5.696754 1.22E-08 2.39012E-06 2.39012E-06

5 0.821597896

AT3G48640.1 38.6603 4.06748 3.248645676 5.692066 1.26E-08 2.43929E-06 2.43929E-06

AT2G38230.1 390.9695 254.156 0.621341776 5.675915 1.38E-08 2.64352E-06 2.64352E-06

AT2G46440.1 54.05935 10.8745 2.313595097 5.658143 1.53E-08 2.91193E-06 2.91193E-06

21.57527

AT1G02205.2 76.6937 -5.623856 1.87E-08 3.52915E-06 3.52915E-06

5 1.829729124

AT4G38550.1 160.5464 77.4153 1.052299688 5.605496 2.08E-08 3.8976E-06 3.8976E-06

AT1G01060.2 250.9395 144.859 0.79269026 5.573683 2.49E-08 4.64935E-06 4.64935E-06

AT2G31880.1 69.6876 19.4327 1.842415606 5.569738 2.55E-08 4.72372E-06 4.72372E-06

AT3G51860.1 98.75402 36.5954 1.432177163 5.559832 2.70E-08 4.96627E-06 4.96627E-06

AT3G27690.1 205.449 339.993 -5.546477 2.91E-08 5.28975E-06 5.28975E-06

0.726724735

AT5G24210.1 156.7978 75.5965 1.052513971 5.54055 3.02E-08 5.43596E-06 5.41274E-06

AT1G49750.1 237.609 135.322 0.812193078 5.533304 3.14E-08 5.5919E-06 5.5919E-06

100.8057

AT5G17230.2 195.917 -5.39198 6.97E-08 1.2163E-05 1.2163E-05

5 0.958664655 AT5G10140.2 0 26.7327 -5.336438 9.48E-08 1.64401E-05 1.64401E-05

6.244617498

26.51290

AT2G03680.2 0 6.578973158 5.334817 9.56E-08 1.64833E-05 1.64833E-05

3

106.9263

AT2G39570.1 44.0233 1.280278223 5.322925 1.02E-07 1.73799E-05 1.73799E-05

5

AT1G24147.1 68.21087 21.088 1.693579388 5.205106 1.94E-07 3.22003E-05 3.22003E-05

AT3G62290.1 424.7795 293.481 0.533447037 5.197502 2.02E-07 3.33432E-05 3.33432E-05

AT1G44575.2 363.722 526.853 -5.167806 2.37E-07 3.88661E-05 3.88661E-05

0.534564293

AT3G02470.1 274.837 170.98 0.684748661 5.164208 2.41E-07 3.93852E-05 3.93852E-05

AT1G56280.1 259.6525 160.063 0.697942262 5.100365 3.39E-07 5.49681E-05 5.49681E-05

AT3G61430.2 274.454 172.013 0.674046764 5.092859 3.53E-07 5.65213E-05 5.65213E-05

AT1G64500.1 320.509 210.531 0.606332188 5.033693 4.81E-07 7.53377E-05 7.53377E-05

AT5G54710.1 113.5198 51.3931 1.14329737 5.029547 4.92E-07 7.65477E-05 7.65477E-05

230.6922

AT3G14210.1 358.291 -5.023831 5.07E-07 7.84171E-05 7.84171E-05

5 0.635162266

23.01831

AT3G02560.2 0 5.941842243 5.015986 5.28E-07 8.12271E-05 8.12271E-05

55

AT3G57260.1 69.732 23.4044 1.57504108 5.002758 5.65E-07 8.65196E-05 8.65196E-05

AT2G24200.2 52.2909 13.6058 1.942338114 4.994795 5.89E-07 8.96662E-05 8.96662E-05

AT5G10760.1 59.45502 17.6958 1.748391642 4.966618 6.81E-07 0.000103148 0.000103148

124.5248

AT5G03240.2 59.8018 1.058172859 4.958404 7.11E-07 0.000107014 0.000107014

5

41.26470

AT3G50470.1 8.2487 2.322669654 4.954745 7.24E-07 0.000108452 0.000108452

5

ATCG00960.1 45.9658 10.5839 2.118689514 4.947975 7.50E-07 0.000111682 0.000111682

AT2G36830.1 289.9395 429.607 -4.941137 7.77E-07 0.000115045 0.000115045

0.567265608

AT2G30520.2 37.51625 6.76536 2.471277018 4.894965 9.83E-07 0.000144083 0.000144083

AT4G38810.1 0 22.3198 -4.817873 1.45E-06 0.000208172 0.000208172

4.864075199

AT3G19030.1 332.064 224.782 0.562934812 4.810701 1.50E-06 0.000214659 0.000214659

AT3G45140.1 231.9285 353.562 -4.790882 1.66E-06 0.000233347 0.000233347

0.608283113

114.0330

AT2G29400.1 54.4958 1.065235068 4.769835 1.84E-06 0.00025778 0.00025778

5

AT4G19200.1 285.5205 187.329 0.608020076 4.762116 1.92E-06 0.000266482 0.000266482

105.4080

AT1G64770.1 189.357 -4.744741 2.09E-06 0.000287489 0.000287489

5 0.845123705

21.61947

AT2G30766.1 65.6063 -1.60150287 -4.724797 2.30E-06 0.000315607 0.000315607

5

AT5G17310.1 0 20.7333 -4.712765 2.44E-06 0.000333168 0.000333168

5.770089403

AT5G11740.1 184.7765 291.719 -4.691011 2.72E-06 0.000366982 0.000366982

0.658798068

AT5G21020.2 203.471 122.787 0.728665363 4.680228 2.87E-06 0.000384926 0.000384926

AT4G13930.1 203.2735 314.319 -4.661021 3.15E-06 0.000418553 0.000418553

0.628807334

AT2G44290.1 88.3886 38.4574 1.200599079 4.608604 4.05E-06 0.00053665 0.00053665

AT5G15230.2 0 20.287 -4.58388 4.56E-06 0.000598442 0.000598442

4.805591229

AT3G02470.4 213.7805 324.954 -4.562984 5.04E-06 0.000654967 0.000654967

0.604105243

AT1G78370.1 267.146 390.688 -4.56103 5.09E-06 0.000657974 0.000657974

0.548388528

AT4G23170.1 35.79804 7.43065 2.268320278 4.550972 5.34E-06 0.000686988 0.000686988

AT2G28000.1 284.429 410.728 -4.525805 6.02E-06 0.000766851 0.000766093

0.530114734 AT3G08030.2 18.8735 0 5.219092044 4.525033 6.04E-06 0.000766093 0.000766093

AT3G18490.1 240.167 154.837 0.633287672 4.520372 6.17E-06 0.000779548 0.000779548

AT1G21310.1 45.48745 12.6007 1.851964679 4.515327 6.32E-06 0.000794677 0.000794677

AT1G02205.1 13.39814 48.3486 -4.502136 6.73E-06 0.000841784 0.000841784

1.851441383

AT3G18410.2 23.48501 66.0838 -1.49255644 -4.497167 6.89E-06 0.000857774 0.000857774

19.06896

AT1G48450.2 0 4.649081679 4.475494 7.62E-06 0.000941018 0.000937454

85

36.26389

AT4G35450.4 86.3329 -4.475345 7.63E-06 0.000937454 0.000937454

5 1.251376562

AT5G59670.1 47.34948 13.9064 1.767599589 4.465194 8.00E-06 0.000978632 0.000978632

243.9390

AT5G63160.1 159.033 0.617194563 4.45775 8.28E-06 0.001008726 0.001008726

5

AT3G16520.2 0 18.6479 -4.434901 9.21E-06 0.00111691 0.00111691

5.111278493

AT1G67860.1 98.82745 47.325 1.06230931 4.430812 9.39E-06 0.001133286 0.001133286

AT5G10140.3 0 18.4917 -4.411873 1.02E-05 0.001226316 0.001226316

6.600416813

AT1G08450.1 106.3937 53.8889 0.981352682 4.316964 1.58E-05 0.001876571 0.001876571

AT5G21940.1 111.3664 57.5503 0.952418673 4.312011 1.62E-05 0.001910843 0.001910843

AT3G16770.1 90.2963 42.6029 1.083715234 4.30219 1.69E-05 0.001980488 0.001980488

186.9533

AT2G47730.1 115.842 0.690519907 4.289131 1.79E-05 0.002091652 0.002091652

5

AT2G32870.1 52.3485 18.072 1.534392023 4.254682 2.09E-05 0.002420559 0.002420559

AT2G07180.1 65.92335 26.8164 1.297673983 4.222174 2.42E-05 0.002774203 0.002774203

103.6963

AT3G02770.1 53.0562 0.966771858 4.211115 2.54E-05 0.002901447 0.002901447

5

AT1G04430.1 38.18775 85.6231 -1.16489015 -4.203648 2.63E-05 0.002986447 0.002986447

75.19192

AT5G66052.1 33.1597 1.181146724 4.19762 2.70E-05 0.00305435 0.00305435

2

AT5G59890.1 141.467 81.7258 0.791602055 4.179953 2.92E-05 0.003288012 0.003288012

15.92978

AT5G28050.2 0 5.770030105 4.175208 2.98E-05 0.003343551 0.003343551

95

115.2520

AT4G37800.1 190.328 -4.13368 3.57E-05 0.003913145 0.003913145

5 0.723695406

AT2G41410.1 81.27165 38.0377 1.09532217 4.115747 3.86E-05 0.004196663 0.004196663

AT5G19120.1 58.0915 22.5763 1.363518019 4.112684 3.91E-05 0.004235938 0.004235938

45.04706

AT2G04450.1 14.5981 1.625652503 4.111409 3.93E-05 0.004242628 0.004242628

5

AT3G47370.1 100.4849 51.7838 0.95640598 4.109687 3.96E-05 0.004257624 0.004257624

AT4G25100.4 55.5167 21.0502 1.399087874 4.097735 4.17E-05 0.004465975 0.004465975

52.02938

AT5G36700.3 104.305 -4.091979 4.28E-05 0.004560498 0.004560498

5 1.003409758

119.6499

AT1G01060.3 66.2419 0.853003835 4.087401 4.36E-05 0.004633368 0.004633368

5

AT2G44120.1 100.9012 52.3321 0.947175274 4.086216 4.38E-05 0.004639102 0.004639102

AT1G21270.1 50.61175 18.1258 1.481427688 4.079207 4.52E-05 0.004762814 0.004762814

AT3G47480.1 27.38465 5.36984 2.350416434 4.064303 4.82E-05 0.005058133 0.005058133

17.79924

AT2G45660.1 1.37645 3.692791937 4.060438 4.90E-05 0.005122967 0.005122967

5

AT5G42080.2 0 16.9984 -4.050554 5.11E-05 0.00532394 0.00532394

4.133182121

AT5G01015.1 37.3 82.16 -4.042615 5.29E-05 0.005486669 0.005486669

1.139260551

AT5G16110.1 97.16485 50.1273 0.954837976 4.035896 5.44E-05 0.005624777 0.005624777

56.48328

AT3G57520.1 22.1192 1.352524789 4.031051 5.55E-05 0.005720415 0.005720415

5 AT2G41100.1 70.8626 31.7054 1.160295822 4.019367 5.84E-05 0.005989128 0.005989128

AT3G25770.1 64.73805 120.424 -3.982575 6.82E-05 0.006944747 0.006944747

0.895437129

14.41986

AT4G25030.2 0 5.75904041 3.972438 7.11E-05 0.007220256 0.007200565

7

AT5G17230.3 146.5025 88.3957 0.728877187 3.972208 7.12E-05 0.007200565 0.007200565

AT5G15850.1 162.436 101.107 0.683988523 3.966248 7.30E-05 0.007355778 0.007355778

AT5G41700.1 114.1155 63.4843 0.846023007 3.965003 7.34E-05 0.007367178 0.007367178

AT2G40000.1 38.24575 11.4828 1.735824964 3.963898 7.37E-05 0.007374398 0.007374398

1.049671

AT3G47810.2 16.6027 -3.958396 7.55E-05 0.00751887 0.00751887

5 3.983408073

53.48717

AT3G57520.3 20.7402 1.366763201 3.953289 7.71E-05 0.007653414 0.007653414

5

AT4G34881.1 103.5325 55.9049 0.889037073 3.93251 8.41E-05 0.00831621 0.00831621

52.86118

AT3G57520.2 20.5203 1.365156944 3.926662 8.61E-05 0.008490297 0.008490297

5

18.13819

AT3G56710.1 1.88024 3.270041927 3.909729 9.24E-05 0.009075384 0.009075384

2

31.90283

AT4G14365.1 8.40803 1.923844911 3.877204 0.0001057 0.010304287 0.010301075

5

AT1G14150.2 40.15836 84.1875 -3.876415 0.000106 0.010301075 0.010301075

1.067905687

AT3G52430.1 45.89365 16.5724 1.469512004 3.861773 0.0001126 0.010899933 0.010899933

AT1G23390.1 119.5275 68.8856 0.795068246 3.856019 0.0001152 0.01112025 0.011088453

AT3G11670.2 0 13.6863 -5.30164053 -3.813825 0.0001368 0.013110567 0.013092323

AT1G65960.2 220.68 314.282 -3.813306 0.0001371 0.013092323 0.013092323

0.510103761

157.5342

AT5G53370.1 99.4493 0.663632402 3.808108 0.00014 0.013278055 0.013278055

5

17.58763

AT1G23850.1 1.94192 3.179005455 3.80328 0.0001428 0.013492917 0.013492917

05

AT5G15410.2 20.09229 52.686 -3.802371 0.0001433 0.013496024 0.013496024

1.390777649

AT5G06320.1 79.2979 39.6311 1.000649649 3.785265 0.0001535 0.014360398 0.014360398

AT5G03240.1 141.4455 87.145 0.698756483 3.767899 0.0001646 0.015292503 0.015292503

AT2G28840.1 44.37275 16.2228 1.451651122 3.763667 0.0001674 0.015501168 0.015501168

AT3G04210.1 74.01495 36.3798 1.024679093 3.726641 0.000194 0.017904078 0.017904078

AT1G61250.1 66.235 30.9537 1.0974819 3.721258 0.0001982 0.018228769 0.018228769

46.92701

AT5G20250.3 18.3344 1.35586564 3.680989 0.0002323 0.021221923 0.021220136

5

18.46846

AT2G40750.1 2.62729 2.813416603 3.679316 0.0002339 0.021220136 0.021220136

5

AT2G25520.1 93.58005 51.169 0.870930961 3.676628 0.0002363 0.021374058 0.021374058

109.0306

AT1G09070.1 63.0193 0.790868119 3.666732 0.0002457 0.022072403 0.022049666

5

20.64979

AT1G21520.1 3.61574 2.513764614 3.666159 0.0002462 0.022049666 0.022049666

5

25.02145

AT2G17040.1 5.72093 2.128844088 3.661394 0.0002508 0.02239076 0.02239076

5

21.89316

AT2G39210.1 4.24149 2.367837468 3.649563 0.0002627 0.023371476 0.023371476

5

AT1G73805.1 25.07119 5.8294 2.104611171 3.639155 0.0002735 0.024257752 0.024257752

AT5G20250.2 42.08793 15.5894 1.432841153 3.630791 0.0002826 0.024896558 0.024896558

AT4G05070.1 135.6605 84.443 0.683950976 3.624432 0.0002896 0.02535408 0.02535408

13.99789

AT3G25882.1 1.05854 3.725062509 3.611788 0.0003041 0.026370423 0.026370423

8

AT2G29670.1 42.95285 16.2893 1.398829252 3.603852 0.0003135 0.027103568 0.027103568

AT2G46430.2 14.68468 1.37024 3.421811317 3.584123 0.0003382 0.02905344 0.02905344 14.52849

AT5G25260.1 1.31569 3.464993356 3.58269 0.0003401 0.029122053 0.029110692

1

AT4G28780.1 30.1687 65.8212 -3.581977 0.000341 0.029110692 0.029110692

1.125499798

11.65867

AT1G32550.2 0 5.556828692 3.570095 0.0003569 0.030274958 0.030274958

05

AT5G17300.1 169.682 112.894 0.587864718 3.567533 0.0003604 0.030478011 0.030478011

AT2G41250.1 166.3665 110.247 0.59362556 3.561839 0.0003683 0.031051045 0.031051045

AT2G21187.2 14.09215 1.21001 3.541800861 3.558328 0.0003732 0.031372456 0.031372456

AT2G37600.2 18.74904 3.11703 2.588572782 3.549742 0.0003856 0.032216004 0.032216004

AT5G05360.2 13.0779 0 3.914093394 3.54862 0.0003873 0.03225517 0.03225517

AT5G05580.1 69.853 120.35 -0.78484216 -3.53931 0.0004012 0.033212696 0.033212696

AT1G55910.1 48.88935 20.728 1.237939304 3.508612 0.0004505 0.037179904 0.037179904

AT1G11260.1 81.46365 43.8517 0.893523683 3.502554 0.0004608 0.037808007 0.037808007

AT5G22620.2 3.196995 19.1645 -3.500783 0.0004639 0.037946547 0.037946547

2.583647966

AT4G02220.1 25.41155 6.55252 1.955362619 3.496985 0.0004705 0.038376276 0.038376276

AT1G68440.1 198.8245 138.371 0.52295393 3.495715 0.0004728 0.038444918 0.038444918

AT3G28540.2 19.66845 3.70323 2.409027208 3.493543 0.0004767 0.038530455 0.038530455

AT5G44570.1 13.66375 1.21859 3.487068772 3.483023 0.0004958 0.039958805 0.039958805

AT4G32870.1 53.45735 23.9963 1.155576349 3.480012 0.0005014 0.040174403 0.040174403

AT3G48115.1 14.44465 1.55023 3.219981065 3.466037 0.0005282 0.042198214 0.042111803

ATCG00490.1 37.93395 14.0194 1.436064998 3.45262 0.0005552 0.044096658 0.044096658

AT1G43670.1 81.3995 133.953 -3.445467 0.0005701 0.044890056 0.044890056

0.718635054

1.280390

AT4G11100.2 13.9348 -3.43839 0.0005852 0.045947797 0.045947797

5 3.444036513

AT1G02930.1 63.4848 31.324 1.019142759 3.436556 0.0005892 0.045996528 0.045996528

AT1G22890.1 55.3102 25.6411 1.109087404 3.428607 0.0006067 0.047230263 0.047230263

AT1G65845.1 24.21185 6.1926 1.967096152 3.426629 0.0006111 0.047440767 0.047440767

AT1G35710.1 35.2188 12.5388 1.489946469 3.41691 0.0006334 0.048890267 0.048890267

C24xCol at ZTO

MPV C24xCol log2(Fold_c

Gene Name z-score p-value q-value q-value

RPKM RPKM hange)

AT2G01020.1 1320.29 5833.45 -54.38968 0 0 0

2.143494519

AT1G29930.1 8620.885 12539.5 -25.1443 1.63E-139 1.4998E-135 1.4998E-135

0.540571938

AT3G41768.1 1553.44 3346.65 -24.99893 6.28E-138 4.33E-134 4.33E-134

1.107251159

513.3905

AT2G14610.1 24.828 4.37001684 22.97246 8.79E-117 4.8478E-113 4.8478E-113

7

ATCG01210.1 602.974 1641.09 -21.71599 1.45E-104 6.6592E-101 6.6592E-101

1.444486661

ATCG00920.1 705.1415 1647.43 -1.22423247 -19.05896 5.54E-81 2.18119E-77 2.18119E-77

ATCG00950.1 906.044 1948.43 -19.02615 1.04E-80 3.57139E-77 3.57139E-77

1.104659083

AT2G25510.1 4023.435 2667.04 0.593188263 17.81468 5.44E-71 1.66599E-67 1.66599E-67

AT5G42530.1 3922.185 2604.56 0.590617913 17.52474 9.28E-69 2.55849E-65 2.55849E-65

AT3G12120.2 2.257155 217.043 -15.09695 1.70E-51 3.34099E-48 3.34099E-48

6.587331591

AT1G75750.1 559.408 1182.7 -1.08011138 -14.53863 6.90E^8 1.2679E-44 1.2679E-44

AT1G29920.1 2344.825 3458.31 -13.62408 2.88E^2 4.96472E-39 4.96472E-39

0.560586942 160.3250

AT5G17920.2 4.22705 5.245204599 13.21932 6.79E-M) 1.10109E-36 1.10109E-36

2

AT4G05050.3 1.039953 157.229 -12.61917 1.66E-36 2.53668E-33 2.53668E-33

7.240205201

ATCGOl 180.1 953.6365 1601.37 -12.19442 3.33E-34 4.58997E-31 4.58997E-31

0.747795324

AT3G22231.1 1308.492 796.735 0.715733246 11.853 2.08E-32 2.60267E-29 2.60267E-29

381.0823

AT1G67865.1 135.319 1.49373837 11.33458 8.85E-30 1.06071E-26 1.06071E-26

5

ATCGOl 160.1 249.744 67.3325 1.891075081 10.78273 4.15E-27 4.58218E-24 4.58218E-24

353.1850

AT2G14560.1 128.82 1.455067557 10.70823 9.31E-27 9.87835E-24 9.87835E-24

2

104.5118

AT1G15690.2 0 7.29687573 10.3829 2.97E-25 3.02978E-22 3.02978E-22

65

239.2091

AT1G14880.1 67.768 1.819596475 10.28901 7.90E-25 7.77978E-22 7.77978E-22

5

AT2G41090.1 675.719 365.51 0.886512544 10.13346 3.93E-24 3.73287E-21 3.61828E-21

AT3G16240.1 897.3015 535.676 0.744232163 10.13319 3.94E-24 3.61828E-21 3.61828E-21

AT1G65490.1 324.7945 127.963 1.343800482 9.689885 3.33E-22 2.86911E-19 2.8691 IE- 19

373.3330

AT5G35630.2 703.086 -9.684476 3.51E-22 2.93343E-19 2.93343E-19

6 0.913237897

AT2G05100.1 674.7335 1081.46 -9.173056 4.60E-20 3.52242E-17 3.52242E-17

0.680590608

AT2G28190.1 92.55565 265.555 -9.084052 1.05E-19 7.7971E-17 7.7971E-17

1.520617727

AT2G10940.1 578.3505 917.874 -8.282628 1.20E-16 8.30723E-14 8.30723E-14

0.666352043

AT5G59890.2 0 63.9149 -8.22571 1.94E-16 1.30521E-13 1.30521E-13

6.359966691

AT3G22235.2 74.05175 212.24 -8.114739 4.87E-16 3.19675E-13 3.19675E-13

1.519090846

AT1G75040.1 134.8691 35.2148 1.937306055 8.048485 8.38E-16 5.37631E-13 5.37631E-13

AT3G51600.1 415.613 693.382 -7.927293 2.24E-15 1.40386E-12 1.40386E-12

0.738409604

AT3G16640.1 889.9105 607.65 0.550419663 7.859076 3.87E-15 2.37166E-12 2.37166E-12

AT1G04040.1 258.055 475.994 -7.734057 1.04E-14 6.11269E-12 6.11269E-12

0.883264804

171.3832

AT5G55450.1 59.4632 1.52715677 7.721292 1.15E-14 6.61646E-12 6.61646E-12

1

ATCG00020.1 80.63565 212.923 -7.632563 2.30E-14 1.29526E-11 1.29526E-11

1.400842081

AT4G05050.1 485.539 287.27 0.757179844 7.558548 4.08E-14 2.24821E-11 2.22577E-11

52.04808

AT2G24790.2 0 5.891228072 7.557277 4.12E-14 2.22577E-11 2.22577E-11

5

AT2G10940.2 407.4455 668.332 -7.541889 4.63E-14 2.45672E-11 2.45672E-11

0.713957856

AT2G05070.1 380.147 630.717 -7.484321 7.19E-14 3.7423 lE-11 3.7423 IE- 11

0.730435414

193.6132

AT1G72930.1 77.8413 1.314569978 7.360252 1.84E-13 9.2045 lE-11 9.2045 lE-11

5

179.0094

AT3G56400.1 69.1124 1.373019266 7.308027 2.71E-13 1.33509E-10 1.33509E-10

5

48.83483

AT1G57720.2 1.91114 4.675405251 7.184789 6.73E-13 3.1464E-10 3.1464E-10

5

68.99072

AT1G65960.1 182.561 -7.07953 1.45E-12 6.4341 lE-10 6.4341 lE-10

5 1.403904273

AT1G56120.1 57.44258 5.79389 3.309516349 7.0137 2.32E-12 1.01603E-09 1.01603E-09

AT2G 18660.1 70.84787 11.3959 2.636209613 6.993992 2.67E-12 1.15131E-09 1.15131E-09

AT4G26850.1 674.307 462.123 0.54512868 6.785379 1.16E-11 4.62777E-09 4.62777E-09

AT5G20630.1 350.177 199.967 0.808322393 6.769836 1.29E-11 5.07959E-09 5.07959E-09

ATCGOl 170.1 130.006 44.9598 1.531870685 6.739541 1.59E-11 6.08606E-09 6.08606E-09

AT1G22930.2 40.75225 0 5.788341314 6.689329 2.24E-11 8.35558E-09 8.35558E-09 AT3G62030.2 699.811 487.304 0.522143277 6.667851 2.60E-11 9.54516E-09 9.54516E-09

AT4G02520.1 507.579 327.674 0.631371178 6.653073 2.87E-11 1.0416E-08 1.0416E-08

AT3G12120.1 359.5715 211.917 0.76277935 6.543498 6.01E-11 2.12487E-08 2.12487E-08

AT1G72930.2 148.6832 60.4789 1.297737839 6.388334 1.68E-10 5.78134E-08 5.78134E-08

ATCG00970.1 158.7295 67.9677 1.223649071 6.315829 2.69E-10 9.14929E-08 9.14929E-08

AT3G11945.2 0 37.0903 -6.281999 3.34E-10 1.12417E-07 1.12417E-07

5.478731688

36.26389

AT4G35450.4 1.15537 4.972107044 6.251073 4.08E-10 1.33838E-07 1.33838E-07

5

557.0393

AT1G54040.1 805.464 -6.236803 4.47E-10 1.44905E-07 1.4357E-07

55 0.532040851

AT4G01150.2 586.0575 840.747 -6.236421 4.48E-10 1.4357E-07 1.4357E-07

0.520629507

AT2G44120.1 100.9012 32.6914 1.625960266 6.190838 5.98E-10 1.87553E-07 1.87553E-07

AT5G27850.1 309.2735 180.082 0.780229237 6.181549 6.35E-10 1.96696E-07 1.96696E-07

AT3G47370.1 100.4849 33.1822 1.598497276 6.105233 1.03E-09 3.14556E-07 3.14556E-07

AT3G09500.1 268.0005 149.878 0.838447061 6.100461 1.06E-09 3.20532E-07 3.20532E-07

AT4G27090.1 434.7615 288.601 0.59114789 5.829111 5.57E-09 1.65247E-06 1.65247E-06

AT5G37260.1 513.717 354.893 0.533589698 5.81702 5.99E-09 1.7576E-06 1.7576E-06

208.1798

AT5G19240.1 109.736 0.923793541 5.811936 6.18E-09 1.77409E-06 1.77409E-06

5

AT3G11120.1 146.7835 66.028 1.152539951 5.802952 6.52E-09 1.83365E-06 1.83365E-06

AT3G50480.1 25.33585 85.7781 -5.752485 8.79E-09 2.40132E-06 2.39033E-06

1.759429129

AT2G27710.1 188.265 96.0242 0.971294872 5.751595 8.84E-09 2.39033E-06 2.39033E-06

AT2G27720.1 186.423 94.9241 0.973733544 5.734753 9.77E-09 2.58962E-06 2.58962E-06

114.5984

AT5G44568.1 45.8336 1.322110334 5.686485 1.30E-08 3.40615E-06 3.40615E-06

75

AT3G62030.1 383.1195 570.84 -5.669191 1.43E-08 3.73287E-06 3.73287E-06

0.575291973

80.03337

AT1G72910.1 24.8386 1.688017889 5.657717 1.53E-08 3.95384E-06 3.95384E-06

5

AT2G37190.1 144.8805 66.512 1.123176872 5.652015 1.59E-08 4.04945E-06 4.04945E-06

151.0934

AT5G49360.1 71.468 1.080071799 5.599836 2.15E-08 5.37927E-06 5.37927E-06

5

AT3G16370.1 343.0555 518.303 -5.585095 2.34E-08 5.80332E-06 5.80332E-06

0.595353748

AT3G51860.1 98.75402 36.9613 1.417823977 5.556559 2.75E-08 6.7752E-06 6.7752E-06

AT3G45030.1 149.773 71.6305 1.064131651 5.511212 3.56E-08 8.69765E-06 8.69765E-06

AT2G38170.3 374.4115 554.421 -5.502178 3.75E-08 9.07514E-06 9.07514E-06

0.566357156

AT5G24530.1 87.90475 30.903 1.508194226 5.480769 4.23E-08 1.01558E-05 1.01558E-05

AT5G42100.2 34.4656 3.40547 3.339231303 5.452807 4.96E-08 1.17879E-05 1.17879E-05

AT3G29240.2 287.539 177.27 0.697809243 5.438993 5.36E-08 1.26303E-05 1.26303E-05

AT3G29240.1 281.814 174.586 0.69080541 5.339966 9.30E-08 2.11888E-05 2.11888E-05

AT3G22890.1 416.275 599.592 -5.32439 1.01E-07 2.28979E-05 2.28979E-05

0.526444216

AT2G38230.1 390.9695 264.62 0.563133961 5.30823 1.11E-07 2.48199E-05 2.48199E-05

AT3G52590.1 375.8795 252.778 0.572399328 5.27614 1.32E-07 2.93435E-05 2.93435E-05

AT1G08830.1 43.88145 109.936 -1.32498079 -5.246866 1.55E-07 3.41336E-05 3.412E-05

AT3G47370.3 0 25.7724 -5.245471 1.56E-07 3.412E-05 3.412E-05

5.771075041

AT5G39740.1 227.42 133.893 0.764278597 5.211975 1.87E-07 4.05738E-05 4.05738E-05

AT5G08760.1 156.9006 80.6245 0.960560656 5.204588 1.94E-07 4.18916E-05 4.18916E-05 AT2G44290.1 88.3886 33.5614 1.39705741 5.200673 1.99E-07 4.2452E-05 4.2452E-05

AT3G62870.1 286.016 180.784 0.661828855 5.192526 2.07E-07 4.40119E-05 4.40119E-05

AT2G36830.1 289.9395 439.645 -5.187487 2.13E-07 4.48739E-05 4.48739E-05

0.600587169

44.51555

AT1G13470.1 8.94291 2.315493248 5.158538 2.49E-07 5.2E-05 5.19405E-05

4

30.95762

AT3G17510.2 87.502 -5.157339 2.50E-07 5.19405E-05 5.19405E-05

35 1.499021267

AT1G08360.1 272.419 170.973 0.672058815 5.13226 2.86E-07 5.8921E-05 5.8921E-05

AT3G60420.1 61.95607 18.5354 1.740962385 5.083495 3.71E-07 7.45945E-05 7.45945E-05

AT3G17390.1 252.017 388.419 -5.059797 4.20E-07 8.38767E-05 8.38767E-05

0.624092719

AT3G01290.1 191.8198 109.023 0.815119121 5.044311 4.55E-07 9.03082E-05 9.03082E-05

22.99125

AT3G09820.2 72.0057 -5.02269 5.10E-07 0.000100373 0.00010036

5 1.647025896

23.01831

AT3G02560.2 0 5.446015819 5.021349 5.13E-07 0.00010036 0.00010036

55

AT4G38810.1 0 23.7423 -4.9532108 -4.973256 6.58E-07 0.000126974 0.000126974

AT2G04780.2 1.06124 24.5109 -4.969085 6.73E-07 0.000127947 0.000127605

4.529600594

AT3G04920.1 149.0955 77.9528 0.935563965 4.96827 6.76E-07 0.000127605 0.000127605

AT5G02960.1 380.5195 265.575 0.518851043 4.890066 1.01E-06 0.000189117 0.000189117

AT4G34670.1 215.8025 130.786 0.722503462 4.848545 1.24E-06 0.000230202 0.000230202

AT3G62250.1 386.0615 271.558 0.507570323 4.835689 1.33E-06 0.000240745 0.000240745

AT3G12965.1 59.713 18.9069 1.659132215 4.829394 1.37E-06 0.000246858 0.000246858

AT3G05590.1 287.902 189.506 0.603334284 4.824459 1.40E-06 0.000251406 0.000251406

AT5G56670.1 232.5995 144.727 0.684513902 4.814745 1.47E-06 0.000262246 0.000262246

141.5520

AT5G54610.1 74.3701 0.928538026 4.811952 1.49E-06 0.000264234 0.000264234

5

AT2G30520.2 37.51625 7.38593 2.344664133 4.770109 1.84E-06 0.000321393 0.000320815

AT5G02450.1 204.3965 123.101 0.731528011 4.76699 1.87E-06 0.000322328 0.000322328

34.66080

AT5G40890.2 88.0257 -4.750545 2.03E-06 0.000347511 0.000347511

5 1.344619633

AT1G27400.1 145.5735 78.323 0.894239823 4.735198 2.19E-06 0.000372556 0.000372556

AT3G54920.1 103.9923 188.33 -4.733029 2.21E-06 0.000374252 0.000374252

0.856786123

AT3G55500.1 57.0985 18.0501 1.661446016 4.727013 2.28E-06 0.000383157 0.000383157

AT4G12600.1 69.45335 25.6882 1.43493848 4.700556 2.59E-06 0.000431054 0.000431054

AT4G23400.1 256.9365 384.372 -4.697305 2.64E-06 0.000435345 0.000435345

0.581091393

AT2G36170.1 122.473 61.8842 0.984820713 4.689864 2.73E-06 0.000448794 0.000448794

AT1G18540.1 194.0175 117.032 0.729283727 4.632662 3.61E-06 0.000589103 0.000589103

AT5G28060.1 242.2155 155.76 0.636966401 4.629099 3.67E-06 0.000595805 0.000595805

AT3G13750.1 211.134 325.144 -4.620852 3.82E-06 0.000616362 0.000616362

0.622919879

AT5G15410.2 20.09229 61.6865 -4.588542 4.46E-06 0.000699428 0.000699428

1.618312789

AT5G16130.1 211.551 132.191 0.678381549 4.557695 5.17E-06 0.000805839 0.000805839

AT5G47560.1 266.595 391.966 -4.543641 5.53E-06 0.000856667 0.000856667

0.556078794

AT4G09320.1 278.3445 187.156 0.572630276 4.5416 5.58E-06 0.000860172 0.000860172

18.87408

AT4G21105.2 0 6.29454374 4.53221 5.84E-06 0.000894321 0.000894321

2

ATCG00490.1 37.93395 89.9628 -4.515378 6.32E-06 0.000957749 0.000957749

1.245838959 AT1G69530.2 120.4594 205.725 -4.499481 6.81E-06 0.001021018 0.001021018

0.772170145

AT3G08030.2 18.8735 0 4.809976145 4.488129 7.19E-06 0.001065369 0.001065369

AT2G25080.1 307.8295 213.45 0.528233332 4.464598 8.02E-06 0.001183082 0.001183082

39.91199

AT2G05540.1 10.0143 1.994760813 4.46076 8.17E-06 0.001198065 0.001198065

5

AT3G49010.3 81.03775 35.6894 1.183098471 4.398715 1.09E-05 0.001572347 0.001572347

AT3G04840.1 282.714 193.69 0.545593856 4.395871 1.10E-05 0.001584779 0.001584779

AT5G19510.1 271.0745 184.105 0.558160597 4.387116 1.15E-05 0.001632896 0.001632896

AT3G48640.1 38.6603 9.73569 1.989497697 4.382897 1.17E-05 0.00165632 0.00165632

AT3G15356.1 64.3508 126.717 -4.364498 1.27E-05 0.001792846 0.001792846

0.977580096

AT3G22235.1 44.22475 97.2191 -4.349407 1.37E-05 0.001911018 0.001911018

1.136385791

AT1G77940.1 205.669 131.147 0.64913955 4.333006 1.47E-05 0.002048743 0.002048743

AT4G 18730.1 200.069 126.868 0.657169421 4.317338 1.58E-05 0.002177669 0.002177669

AT5G58420.1 176.348 107.89 0.708864062 4.314739 1.60E-05 0.002192487 0.002192487

AT1G48920.1 65.83735 26.4087 1.317892983 4.299856 1.71E-05 0.002333412 0.002333412

AT2G14560.2 30.43515 6.06344 2.327530054 4.278229 1.88E-05 0.002559356 0.002559356

AT2G37600.2 18.74904 1.20669 3.957689729 4.27416 1.92E-05 0.002593766 0.002593766

AT5G25610.1 177.222 273.151 -4.243191 2.20E-05 0.002950199 0.002950199

0.624140996

AT1G56110.1 43.8299 13.3493 1.715151294 4.232604 2.31E-05 0.003077667 0.003077667

AT3G61430.2 274.454 190.091 0.529874138 4.226514 2.37E-05 0.00314692 0.00314692

AT4G25100.4 55.5167 20.4359 1.441816033 4.217103 2.47E-05 0.003265422 0.003265422

AT1G56045.1 163.396 99.164 0.720484294 4.209152 2.56E-05 0.003366351 0.003366351

AT3G23450.1 38.83625 87.0084 -4.197453 2.70E-05 0.003511554 0.003511554

1.163750787

AT5G42080.2 0 18.1007 -4.19583 2.72E-05 0.003520197 0.003520197

4.223828655

15.92978

AT5G28050.2 0 5.634943973 4.181894 2.89E-05 0.003708295 0.003708295

95

19.06896

AT1G48450.2 1.55976 3.611830842 4.180818 2.90E-05 0.003708643 0.003708643

85

AT1G69530.1 94.2991 164.305 -4.158935 3.20E-05 0.004063582 0.004063582

0.801060477

AT1G48630.1 72.4728 31.9923 1.179714919 4.15069 3.31E-05 0.004193453 0.00417733

AT5G52650.1 170.0575 105.595 0.687481111 4.131466 3.60E-05 0.004518647 0.004518647

AT5G03850.1 194.262 125.166 0.634160997 4.130368 3.62E-05 0.004519753 0.004519753

AT1G24147.1 68.21087 29.3735 1.215486483 4.119213 3.80E-05 0.004701638 0.004701638

AT3G16520.2 0 16.274 -4.112524 3.91E-05 0.004818411 0.004818411

4.914834216

AT2G27530.1 223.618 149.677 0.579183774 4.10944 3.97E-05 0.004861499 0.004861499

AT1G70600.1 278.658 196.082 0.507038468 4.104508 4.05E-05 0.00494441 0.00494441

AT1G52300.1 272.9655 191.31 0.512806334 4.10104 4.11E-05 0.004996997 0.004996997

AT3G60770.1 232.785 158.299 0.556345956 4.054362 5.03E-05 0.00608084 0.006064841

AT2G47880.1 20.3154 2.37149 3.098708081 4.053955 5.04E-05 0.006064841 0.006064841

17.72281

AT3G02360.2 1.46184 3.599750167 4.025633 5.68E-05 0.006813485 0.006813485

15

AT5G09510.1 64.91725 27.9302 1.216775982 4.021755 5.78E-05 0.006896688 0.006896688

AT1G64650.2 0 15.5573 -4.017782 5.87E-05 0.006983714 0.006983714

4.890524371

AT1G49750.1 237.609 163.466 0.539598888 3.992822 6.53E-05 0.00772822 0.00772822 AT3G44010.1 190.605 124.586 0.613444006 3.979949 6.89E-05 0.008124024 0.008100246

AT5G59850.1 114.701 64.2739 0.83557305 3.979632 6.90E-05 0.008100246 0.008100246

AT1G21250.1 100.4144 53.5949 0.905798547 3.973319 7.09E-05 0.008282833 0.008282833

AT4G15440.1 102.2531 55.143 0.890894919 3.956599 7.60E-05 0.00884672 0.0088253

AT5G10760.1 59.45502 24.8078 1.261004827 3.953795 7.69E-05 0.008876198 0.008876198

AT2G20450.1 72.14735 33.6266 1.10134349 3.928384 8.55E-05 0.009827157 0.009827157

AT3G57260.1 69.732 31.9689 1.125151756 3.92635 8.62E-05 0.009869465 0.009833395

AT4G31500.1 96.3199 51.0496 0.915934237 3.926235 8.63E-05 0.009833395 0.009833395

AT5G48490.1 45.379 16.1631 1.489320891 3.902581 9.52E-05 0.010801477 0.010757439

AT5G10360.1 220.6445 150.827 0.548829073 3.902576 9.52E-05 0.010757439 0.010757439

AT3G44750.1 23.40085 4.1588 2.492321723 3.899471 9.64E-05 0.010851857 0.010851857

AT5G56710.1 124.4825 72.8395 0.773150015 3.892375 9.93E-05 0.011128866 0.011128866

AT3G11945.1 90.1307 47.2517 0.931652361 3.849896 0.0001182 0.013035841 0.013035841

AT2G44120.2 8.63801 33.2949 -3.847175 0.0001195 0.01312889 0.01312889

1.946530315

105.9526

AT1G69530.3 173.5 -3.829481 0.0001284 0.013998094 0.013998094

5 0.711515991

AT1G78450.1 35.23955 76.7712 -3.827718 0.0001293 0.014043241 0.014043241

1.123369697

AT2G21187.2 14.09215 0 4.473193702 3.825792 0.0001304 0.014097969 0.014097969

AT5G59670.1 47.34948 17.8888 1.404291969 3.820745 0.000133 0.014333472 0.014333472

AT2G32870.1 52.3485 21.158 1.306944938 3.810461 0.0001387 0.01488493 0.01488493

AT1G69530.4 105.7521 172.769 -3.804492 0.0001421 0.01518936 0.01518936

0.708158065

AT3G18410.2 23.48501 58.2358 -3.784366 0.0001541 0.016409089 0.016409089

1.310166103

AT5G64140.1 102.1007 56.925 0.842858466 3.781109 0.0001561 0.016561341 0.016561341

13.05923

AT1G47740.2 0 5.385887761 3.77991 0.0001569 0.016577509 0.016577509

5

AT3G53020.1 181.1825 120.02 0.594168781 3.778556 0.0001577 0.016604256 0.016604256

AT1G04480.1 234.1695 164.93 0.505699338 3.754208 0.0001739 0.018165594 0.018165594

AT1G36050.1 13.0713 0 4.893381694 3.744774 0.0001806 0.018720188 0.018720188

AT2G01250.1 162.074 104.962 0.626785556 3.736171 0.0001868 0.019299472 0.019299472

AT5G21940.1 111.3664 64.559 0.78662389 3.733817 0.0001886 0.019408172 0.019408172

17.79924

AT2G45660.1 2.27088 2.97049267 3.721399 0.0001981 0.020237093 0.020222273

5

AT5G62190.1 88.55875 47.3931 0.901957827 3.718706 0.0002002 0.020303584 0.020303584

AT5G19120.1 58.0915 25.6563 1.179013954 3.71441 0.0002037 0.020575987 0.020575987

AT3G48930.1 207.13 142.955 0.534975443 3.701151 0.0002146 0.021602538 0.021602538

AT3G14310.1 100.6882 164.133 -3.692507 0.0002221 0.022188544 0.022188544

0.704970712

AT3G16530.1 57.4174 106.35 -3.677332 0.0002357 0.023465705 0.023465705

0.889260125

124.5248

AT5G03240.2 75.7706 0.716723596 3.658693 0.0002535 0.025058861 0.025058861

5

46.51102

AT5G10140.1 90.8146 -3.654291 0.0002579 0.02540157 0.025334657

46 0.965351532

AT4G31700.1 135.561 84.6313 0.679678952 3.654053 0.0002581 0.025334657 0.025334657

AT3G20470.1 83.5314 140.814 -3.638762 0.000274 0.026791934 0.026791934

0.753400253

AT5G60920.1 124.1495 192.674 -3.618052 0.0002968 0.028926624 0.028926624

0.634083452

AT1G67860.1 98.82745 56.3478 0.810552532 3.60409 0.0003132 0.030419272 0.030419272 AT3G61110.1 187.7985 128.354 0.549057283 3.601594 0.0003163 0.03060512 0.03060512

AT4G10450.1 40.65705 15.0543 1.433329913 3.593464 0.0003263 0.031466109 0.031466109

AT5G24210.1 156.7978 102.897 0.607704397 3.581602 0.0003415 0.032701626 0.032701626

AT2G47610.1 162.9155 108.022 0.592798706 3.576102 0.0003488 0.03328144 0.03328144

AT1G14150.2 40.15836 80.6448 -3.569357 0.0003579 0.034032354 0.034032354

1.005881158

AT1G34030.1 136.7185 87.2324 0.648272486 3.528769 0.0004175 0.039297406 0.039292763

103.6963

AT3G02770.1 60.9533 0.766588878 3.527899 0.0004189 0.039292763 0.039292763

5

AT4G25100.1 57.6868 104.39 -3.519513 0.0004323 0.04028205 0.04028205

0.855670375

AT1G76160.1 144.3505 215.75 -3.511233 0.000446 0.041140921 0.041140921

0.579784455

115.2520

AT4G37800.1 179.33 -3.51022 0.0004477 0.041160375 0.041160375

5 0.637824444

AT1G15405.1 33.79175 70.5434 -3.497764 0.0004692 0.042988067 0.042988067

1.061840045

AT3G06680.2 45.4426 18.7566 1.276647046 3.488671 0.0004854 0.044329887 0.044329887

ColxC24 at ZT6

MPV ColxC24 log2(Fold_c

Gene Name z-score p-value q-value q-value

RPKM RPKM hange)

AT2G34430.1 3462.985 6992.6 -33.22218 5.15E-242 1.4153E-237 1.4153E-237

1.013812842

AT1G67090.1 14717.8 19905.4 -24.95106 2.08E-137 2.8571E-133 2.8571E-133

0.435597828

AT1G66100.1 679.961 1761.73 -1.37346893 -21.46937 3.01E-102 2.75799E-98 2.75799E-98

458.2150

AT2G42540.2 30.3444 3.916522645 21.11586 5.69E-99 3.90669E-95 3.90669E-95

5

AT2G34420.1 8960.135 12360 -20.93067 2.81E-97 1.54714E-93 1.54714E-93

0.464086369

441.3380

AT2G42540.1 29.7895 3.889008321 20.67792 5.47E-95 2.50758E-91 2.50758E-91

5

346.5066

AT5G59320.1 3.5468 6.610221249 19.33086 2.95E-83 1.01489E-79 1.01489E-79

5

AT4G 18440.1 566.515 1400.57 -1.30582803 -18.37603 2.04E-75 6.2405E-72 6.2405E-72

AT4G04020.1 537.8893 103.833 2.373044259 18.26353 1.62E-74 4.43853E-71 4.43853E-71

324.0262

AT5G35630.2 0 8.560251691 17.23648 1.41E-66 3.53255E-63 3.53255E-63

65

1315.334

AT5G11740.1 675.18 0.96208566 15.24272 1.84E-52 3.61272E-49 3.61272E-49

5

AT5G25610.1 618.1075 206.122 1.584359274 15.1416 8.61E-52 1.57734E-48 1.57734E-48

AT5G01530.1 4531.32 6244.14 -14.78162 1.92E^9 3.3058E-46 3.3058E-46

0.462571507

AT3G27690.1 1939.925 3065.26 -14.77739 2.05E^9 3.31323E-46 3.31323E-46

0.660008574

AT1G55670.1 3702.25 5138.19 -13.71138 8.68E^3 1.25536E-39 1.25536E-39

0.472857921

AT2G38170.3 255.224 37.0744 2.783268749 13.69964 1.02E^2 1.40202E-39 1.40202E-39

AT3G22231.1 178.0048 547.966 -13.61818 3.12E-12 4.08639E-39 4.08639E-39

1.622170235

AT3G61470.1 4325.365 5780.87 -12.79598 1.73E-37 2.0629E-34 2.0629E-34

0.418464748

AT1G15820.1 4339.485 5773.43 -12.57418 2.93E-36 3.35281E-33 3.35281E-33

0.411904843

AT1G20440.1 160.9686 11.6055 3.793898706 12.38847 3.02E-35 3.1893E-32 3.1893E-32

AT3G23810.1 566.5985 240.548 1.236001978 12.1176 8.52E-34 8.67345E-31 8.67345E-31

AT2G23672.1 5.192375 142.125 -12.06963 1.53E-33 1.49996E-30 1.49996E-30

4.774621959

134.4629

AT5G59310.1 0 7.109524035 11.87158 1.66E-32 1.57594E-29 1.57594E-29

03 AT1G37130.1 504.3845 206.333 1.289549364 11.7986 3.97E-32 3.63515E-29 3.63515E-29

AT5G03350.1 23.60015 186.792 -11.71978 1.01E-31 8.94791E-29 8.94791E-29

2.984564734

175.7573

AT5G24780.1 477.698 -11.61772 3.35E-31 2.87647E-28 2.87647E-28

5 1.442513814

AT3G17390.1 666.1755 332.394 1.003007956 11.2034 3.92E-29 3.26768E-26 3.26768E-26

109.3148

AT5G52310.1 2.94445 5.214347545 10.93057 8.23E-28 6.6545E-25 6.6545E-25

6

391.8129

AT5G24770.1 777.359 -10.8053 3.25E-27 2.55095E-24 2.55095E-24

5 0.988415936

105.2391

AT2G38530.1 3.0328 5.116877192 10.70561 9.58E-27 7.31304E-24 7.31304E-24

4

AT2G23670.1 414.1405 173.237 1.257373201 10.49241 9.36E-26 6.95279E-23 6.95279E-23

AT2G30570.1 2429.63 3320.6 -10.4757 1.12E-25 8.07832E-23 8.07832E-23

0.450707319

AT1G07600.1 412.678 173.04 1.253910959 10.4524 1.43E-25 1.00666E-22 1.00666E-22

AT5G24780.2 132.5342 363.795 -10.21656 1.67E-24 1.09375E-21 1.09375E-21

1.456761024

AT3G62030.1 624.871 1059.55 -9.952409 2.46E-23 1.50322E-20 1.50322E-20

0.761821378

AT1G67740.1 1726.13 2436.67 -9.93445 2.95E-23 1.76117E-20 1.76117E-20

0.497369757

AT2G38170.1 178.079 41.0644 2.116557273 9.822587 9.00E-23 5.26251E-20 5.26251E-20

AT4G05180.1 1944.87 2681.19 -9.682862 3.57E-22 2.04155E-19 2.04155E-19

0.463199733

AT3G09390.1 205.567 57.7938 1.830622048 9.626382 6.19E-22 3.46989E-19 3.46989E-19

AT1G07610.1 121.0873 16.9464 2.836996826 9.525739 1.64E-21 9.00672E-19 9.00672E-19

AT3G22235.1 12.89104 116.145 -9.516719 1.79E-21 9.63094E-19 9.63094E-19

3.171486483

AT5G13930.1 427.8275 204.013 1.068368134 9.426963 4.22E-21 2.18881E-18 2.18881E-18

AT2G01020.1 203.1045 451.654 -9.407713 5.07E-21 2.55673E-18 2.53349E-18

1.152995781

AT3G41979.1 203.1045 451.654 -9.407713 5.07E-21 2.55673E-18 2.53349E-18

1.152995781

503.8607

AT3G14210.1 867.557 -9.249251 2.26E-20 1.08994E-17 1.08994E-17

5 0.783933471

342.2011

AT5G15970.1 156.451 1.129133728 8.795495 1.42E-18 6.63357E-16 6.63357E-16

5

AT1G14250.1 148.4455 350.021 -8.786624 1.54E-18 7.05903E-16 6.96765E-16

1.237508121

AT4G17470.1 47.4905 179.262 -8.786231 1.55E-18 6.96765E-16 6.96765E-16

1.916358847

AT3G16140.1 1447.015 2013.51 -0.47663276 -8.641076 5.57E-18 2.46824E-15 2.46824E-15

264.4387

AT5G24770.2 508.632 -0.94368863 -8.387459 4.97E-17 2.13306E-14 2.13306E-14

5

AT2G36830.1 703.7615 442.005 0.671023903 8.353332 6.64E-17 2.80576E-14 2.77599E-14

AT5G02160.1 264.548 507.659 -8.352789 6.67E-17 2.77599E-14 2.77599E-14

0.940330238

AT4G08870.1 693.13 434.565 0.673553978 8.315383 9.15E-17 3.75123E-14 3.75123E-14

AT5G06870.1 72.55255 214.68 -8.295107 1.08E-16 4.38434E-14 4.38434E-14

1.565089567

AT2G46820.1 1529.07 2085.93 -0.44803629 -8.246223 1.63E-16 6.51101E-14 6.51101E-14

AT3G08580.2 650.6945 405.147 0.683534902 8.153619 3.53E-16 1.38658E-13 1.38658E-13

AT3G50820.1 1681.18 2255.8 -8.094834 5.73E-16 2.21947E-13 2.21947E-13

0.424164965

AT4G17090.1 186.248 66.0338 1.495948358 7.982347 1.44E-15 5.40497E-13 5.40497E-13

AT2G01021.1 174.4355 366.853 -7.969921 1.59E-15 5.89638E-13 5.89638E-13

1.072508405

62.71857

AT2G14610.1 3.48079 4.171406053 7.954437 1.80E-15 6.59352E-13 6.59352E-13

3

137.0262

AT1G01470.1 38.2337 1.841535569 7.889879 3.02E-15 1.09375E-12 1.09375E-12

5 AT1G19670.1 164.022 348.704 -7.867538 3.62E-15 1.29085E-12 1.29085E-12

1.088113579

AT4G35090.1 598.303 371.403 0.687890828 7.858764 3.88E-15 1.3668E-12 1.3668E-12

AT2G43550.1 257.5345 481.592 -7.845147 4.32E-15 1.50432E-12 1.50432E-12

0.903045714

AT3G22235.2 28.66485 124.359 -7.828232 4.95E-15 1.69963E-12 1.69963E-12

2.117156286

AT1G77760.1 88.24675 14.3045 2.625074076 7.816977 5.41E-15 1.8357E-12 1.8357E-12

134.0233

AT2G42530.1 37.4585 1.83911934 7.796238 6.38E-15 2.13748E-12 2.13748E-12

5

AT1G07590.1 234.388 99.5185 1.235862064 7.792126 6.59E-15 2.18162E-12 2.18162E-12

AT3G14420.4 37.59345 141.037 -7.768253 7.96E-15 2.60336E-12 2.60336E-12

1.907520468

AT1G79850.1 648.4465 987.261 -7.713707 1.22E-14 3.95127E-12 3.95127E-12

0.606443987

AT1G13930.1 804.3055 547.062 0.556039239 7.658241 1.88E-14 6.02334E-12 6.02334E-12

AT3G13520.1 355.206 188.352 0.915224599 7.613058 2.68E-14 8.45548E-12 8.45548E-12

AT1G78370.1 913.699 1306.39 -7.545667 4.50E-14 1.40521E-11 1.4052 IE- 11

0.515794771

1242.907

AT1G54040.2 1701.42 -7.532904 4.96E-14 1.53227E-11 1.53227E-11

3 0.453020618

AT3G09820.2 0 62.9915 -7.417464 1.19E-13 3.64532E-11 3.64532E-11

8.778646427

AT4G34950.1 260.2055 123.433 1.075923304 7.391307 1.45E-13 4.39071E-11 4.39071E-11

AT5G23820.1 87.83275 218.543 -7.297504 2.93E-13 8.66256E-11 8.66256E-11

1.315086289

AT1G20620.2 53.6408 160.841 -7.24653 4.28E-13 1.25004E-10 1.25004E-10

1.584232552

AT4G27440.2 228.739 423.272 -7.242854 4.39E-13 1.27089E-10 1.27089E-10

0.887882689

AT1G75040.1 97.9543 23.1209 2.082911268 7.213694 5.45E-13 1.55881E-10 1.55881E-10

AT3G11630.1 963.286 1345.9 -7.15292 8.50E-13 2.33454E-10 2.33454E-10

0.482535119

122.0934

AT5G49480.1 36.8416 1.728578183 7.141309 9.24E-13 2.51536E-10 2.51536E-10

5

AT4G27440.1 248.0635 445.29 -0.84403574 -7.093706 1.31E-12 3.45012E-10 3.45012E-10

AT1G09750.1 174.089 342.429 -0.97597984 -7.09046 1.34E-12 3.49837E-10 3.49837E-10

211.3350

AT2G46820.2 94.281 1.164493093 7.075383 1.49E-12 3.86385E-10 3.86385E-10

5

45.69840

AT1G57720.2 1.64845 4.792961778 6.994116 2.67E-12 6.85576E-10 6.85576E-10

5

AT2G15620.1 333.763 184.809 0.852789014 6.977554 3.00E-12 7.64285E-10 7.64285E-10

AT3G14420.6 292.068 155.532 0.909092861 6.866642 6.57E-12 1.64214E-09 1.64214E-09

109.4150

AT2G46600.1 32.6081 1.746508908 6.807293 9.95E-12 2.4622E-09 2.4622E-09

5

AT5G57560.1 50.4339 4.27188 3.561450765 6.78277 1.18E-11 2.8671E-09 2.8671E-09

AT1G20450.1 64.6089 10.0933 2.678334973 6.759049 1.39E-11 3.34834E-09 3.34834E-09

ATCG01210.1 348.6355 567.636 -6.751578 1.46E-11 3.49476E-09 3.49476E-09

0.703246609

AT4G35090.2 422.0065 258.767 0.705611575 6.737302 1.61E-11 3.82256E-09 3.82256E-09

AT1G10370.1 223.5145 108.442 1.0434448 6.690312 2.23E-11 5.23069E-09 5.23069E-09

AT4G17340.1 182.009 79.9321 1.187162893 6.662373 2.69E-11 6.27502E-09 6.27502E-09

AT4G16980.1 814.2085 1141.36 -6.65324 2.87E-11 6.62108E-09 6.62108E-09

0.487283721

AT5G42530.1 936.878 1287.67 -6.63978 3.14E-11 7.19435E-09 7.19435E-09

0.458829814

AT2G38140.1 350.1345 562.886 -6.555454 5.55E-11 1.24955E-08 1.24055E-08

0.684933544

AT1G62180.1 291.283 160.608 0.858877748 6.555315 5.55E-11 1.24055E-08 1.24055E-08 AT3G22600.1 38.85114 0 5.55738228 6.540333 6.14E-11 1.36035E-08 1.36035E-08

38.67408

AT3G02560.2 0 5.653373321 6.527623 6.68E-11 1.46906E-08 1.46906E-08

45

AT3G56290.1 164.4246 69.9576 1.232873459 6.514408 7.30E-11 1.59165E-08 1.59161E-08

AT3G55500.1 60.69725 9.68021 2.648520904 6.513225 7.36E-11 1.59161E-08 1.59161E-08

AT1G02820.1 67.21645 12.655 2.409106842 6.510667 7.48E-11 1.6063E-08 1.6063E-08

AT2G24200.2 89.89505 24.342 1.884793969 6.487731 8.71E-11 1.85633E-08 1.85633E-08

AT2G45470.1 309.285 507.531 -6.481844 9.06E-11 1.91539E-08 1.91539E-08

0.714559079

AT1G48920.1 227.9215 115.252 0.983745235 6.45365 1.09E-10 2.29053E-08 2.29053E-08

AT1G03600.1 533.516 789.738 -6.439919 1.20E-10 2.48863E-08 2.48863E-08

0.565842573

AT1G20070.1 55.13055 7.67472 2.844665972 6.435973 1.23E-10 2.53496E-08 2.53496E-08

1167.055

AT4G21280.1 1547.88 -6.421444 1.35E-10 2.76835E-08 2.76835E-08

5 0.407420459

AT1G03820.1 45.00694 4.19056 3.424932525 6.312853 2.74E-10 5.49494E-08 5.49494E-08

AT1G15690.1 364.2845 223.12 0.707245771 6.271195 3.58E-10 7.13484E-08 7.13484E-08

AT5G40890.2 34.8311 0 6.019060306 6.187222 6.12E-10 1.21062E-07 1.21062E-07

AT3G08030.1 92.44265 28.3651 1.704441667 6.155276 7.49E-10 1.47116E-07 1.47116E-07

AT1G20450.2 53.9998 8.58684 2.652754849 6.148499 7.82E-10 1.5245E-07 1.5245E-07

AT5G04140.1 338.0065 204.99 0.721497458 6.139152 8.30E-10 1.60557E-07 1.60557E-07

58.48607

AT5G55450.1 10.8282 2.433299733 6.107217 1.01E-09 1.94833E-07 1.94833E-07

5

AT2G36530.1 466.2445 311.729 0.580794332 6.041838 1.52E-09 2.90781E-07 2.90781E-07

AT2G20260.1 646.1025 910.553 -6.038504 1.56E-09 2.94804E-07 2.94804E-07

0.494979936

AT1G13440.1 637.5915 457.379 0.479242269 6.034334 1.60E-09 3.00446E-07 3.00446E-07

AT5G47330.1 35.02124 1.70094 4.36382601 6.011057 1.84E-09 3.44575E-07 3.44575E-07

AT3G08940.2 908.204 1218.59 -5.945919 2.75E-09 5.10461E-07 5.10461E-07

0.424124511

AT3G51860.1 32.48985 1.10451 4.878510697 5.913184 3.36E-09 6.14761E-07 6.14761E-07

AT3G22121.1 155.86 286.782 -5.911594 3.39E-09 6.16618E-07 6.16618E-07

0.879703754

AT1G27950.1 270.783 156.966 0.786685074 5.891805 3.82E-09 6.90639E-07 6.90639E-07

AT5G01410.1 289.716 171.773 0.754136076 5.890507 3.85E-09 6.91536E-07 6.91536E-07

AT2G41410.1 70.83545 18.3246 1.950689839 5.887499 3.92E-09 6.99667E-07 6.99667E-07

88.25640

AT4G16590.1 28.1462 1.648760849 5.87991 4.10E-09 7.27785E-07 7.27785E-07

65

AT1G23310.1 547.1075 384.62 0.508390549 5.867442 4.43E-09 7.79631E-07 7.79631E-07

AT1G19960.1 18.65192 74.784 -5.850583 4.90E-09 8.57428E-07 8.55536E-07

2.003405492

AT3G11945.2 31.05615 0 5.723547275 5.849895 4.92E-09 8.55536E-07 8.55536E-07

AT4G30270.1 93.204 31.5099 1.564586696 5.82753 5.63E-09 9.72269E-07 9.72269E-07

AT5G15960.1 38.48454 3.71908 3.371261316 5.801074 6.59E-09 1.13173E-06 1.13173E-06

30.74252

AT4G35450.4 0 5.249077763 5.79978 6.64E-09 1.13341E-06 1.13341E-06

3

AT2G37170.1 176.47 87.9734 1.00428367 5.770776 7.89E-09 1.33855E-06 1.33855E-06

AT5G44130.1 167.5135 82.2992 1.025327056 5.713713 1.11E-08 1.84101E-06 1.84101E-06

AT2G38230.1 219.5125 121.296 0.85577112 5.674136 1.39E-08 2.30758E-06 2.30758E-06

AT5G06290.1 248.677 403.604 -5.657232 1.54E-08 2.53144E-06 2.53144E-06

0.698667393

AT3G09820.1 214.831 118.334 0.860337537 5.637118 1.73E-08 2.82858E-06 2.82858E-06 AT2G44120.1 124.886 53.7443 1.216428098 5.62054 1.90E-08 3.09545E-06 3.09545E-06

AT3G54210.1 696.1735 952.059 -5.614421 1.97E-08 3.18815E-06 3.18815E-06

0.451604083

AT1G23310.2 385.4955 255.963 0.590778739 5.570834 2.54E-08 4.0743E-06 4.0743E-06

AT3G51600.1 406.4395 274.186 0.567885709 5.537902 3.06E-08 4.89091E-06 4.89091E-06

ATCG00950.1 561.683 787.314 -5.523682 3.32E-08 5.27329E-06 5.27329E-06

0.487182994

AT3G02470.4 223.374 126.727 0.817737338 5.51985 3.39E-08 5.35862E-06 5.35862E-06

159.0129

AT5G15650.1 78.9837 1.009517406 5.500066 3.80E-08 5.96179E-06 5.96179E-06

5

34.41927

AT2G36590.1 3.433 3.325674947 5.455964 4.87E-08 7.60533E-06 7.60533E-06

5

AT1G56070.1 654.826 491.372 0.414295955 5.426212 5.76E-08 8.93718E-06 8.93603E-06

AT2G33450.1 447.3685 644.968 -5.425228 5.79E-08 8.93603E-06 8.93603E-06

0.527763906

AT1G70600.1 539.2125 390.436 0.465767859 5.420499 5.94E-08 9.12446E-06 9.12446E-06

AT1G23130.1 536.9025 389.053 0.464693418 5.398615 6.72E-08 1.01964E-05 1.01964E-05

AT4G21960.1 466.8715 667.543 -5.39265 6.94E-08 1.04829E-05 1.04434E-05

0.515835249

AT5G01600.1 227.489 368.688 -5.391624 6.98E-08 1.04854E-05 1.04434E-05

0.696603674

ATCG00920.1 210.067 346.045 -5.391366 6.99E-08 1.04434E-05 1.04434E-05

0.720110117

AT2G37180.1 96.4126 37.3495 1.368132772 5.386281 7.19E-08 1.0685E-05 1.0685E-05

26.51602

AT2G05540.1 0 5.222932109 5.38413 7.28E-08 1.07554E-05 1.07554E-05

4

AT5G58250.1 228.457 369.187 -5.364301 8.13E-08 1.18787E-05 1.18787E-05

0.692429105

AT5G36700.2 60.8255 16.4314 1.888220867 5.342868 9.15E-08 1.32324E-05 1.32324E-05

AT3G02480.1 25.8883 0 5.694228326 5.340986 9.24E-08 1.33006E-05 1.32463E-05

145.3564

AT3G20470.1 71.1875 1.029899249 5.340781 9.25E-08 1.32463E-05 1.32463E-05

5

AT1G55330.1 447.538 314.794 0.507602142 5.299718 1.16E-07 1.65145E-05 1.65145E-05

AT4G37990.1 25.3133 0 5.661823693 5.281119 1.28E-07 1.81881E-05 1.81881E-05

AT4G22240.1 243.3315 146.883 0.728255663 5.248123 1.54E-07 2.15441E-05 2.15441E-05

AT4G18100.1 465.2135 331.925 0.487035668 5.222824 1.76E-07 2.45815E-05 2.45815E-05

26.36685

AT2G43620.1 0 7.476711596 5.185855 2.15E-07 2.96941E-05 2.96941E-05

5

AT1G78040.2 336.089 224.307 0.583368681 5.14798 2.63E-07 3.59997E-05 3.59997E-05

77.32357

AT1G15690.2 159.663 -5.14489 2.68E-07 3.6416E-05 3.6416E-05

45 1.046049787

26.73460

AT4G 14690.1 1.66485 4.005244257 5.13467 2.83E-07 3.82621E-05 3.82621E-05

5

AT1G51402.1 44.57105 109.876 -5.131281 2.88E-07 3.87668E-05 3.87668E-05

1.301697442

AT5G42650.1 434.275 617.961 -5.117311 3.10E-07 4.15463E-05 4.15463E-05

0.508906888

AT2G06850.1 130.2085 63.0986 1.04514373 5.112615 3.18E-07 4.23859E-05 4.23859E-05

AT1G73325.1 59.1657 131.923 -5.098207 3.43E-07 4.5521E-05 4.5521E-05

1.156863159

ATCGOl 180.1 739.6015 980.298 -5.096085 3.47E-07 4.58125E-05 4.58125E-05

0.406472229

110.2576

AT5G04140.2 205.117 -5.081002 3.75E-07 4.93672E-05 4.93672E-05

75 0.895567982

AT4G00430.1 221.075 132.169 0.742152054 5.079338 3.79E-07 4.95643E-05 4.95643E-05

AT3G08940.1 716.4695 952.318 -5.076527 3.84E-07 5.00645E-05 5.00645E-05

0.410538111

AT3G51240.1 49.32555 11.9124 2.049871034 5.068548 4.01E-07 5.19626E-05 5.19626E-05

AT1G28400.1 69.54185 23.7105 1.552355361 5.006171 5.55E-07 7.13009E-05 7.13009E-05 AT1G02930.1 25.4132 1.66288 3.933822082 4.97925 6.38E-07 8.1588E-05 8.1588E-05

AT5G09440.1 24.29697 1.30663 4.216853837 4.964953 6.87E-07 8.74275E-05 8.74275E-05

AT3G08030.2 0 23.2043 -4.931059 8.18E-07 0.000102627 0.000102527

5.134820866

AT1G78570.1 102.9068 46.0761 1.159247804 4.920211 8.65E-07 0.0001075 0.0001075

AT2G05520.2 126.8635 63.0189 1.009420573 4.912267 9.00E-07 0.000111446 0.000111446

AT2G37660.1 277.9155 419.075 -4.91071 9.07E-07 0.000111831 0.000111831

0.592562161

AT3G14395.1 50.615 13.5515 1.901112447 4.895288 9.82E-07 0.000120428 0.000120428

AT3G27830.1 513.994 705.21 -4.884054 1.04E-06 0.000126933 0.000126933

0.456301414

AT1G29070.1 536.027 731.077 -4.874983 1.09E-06 0.000132319 0.000132319

0.447717693

AT3G25520.1 515.7785 385.653 0.419448303 4.864482 1.15E-06 0.000138927 0.000138927

AT2G16630.1 48.0092 12.4335 1.949078427 4.844358 1.27E-06 0.0001531 0.0001531

173.2718

AT4G23600.2 98.6053 0.813300193 4.84032 1.30E-06 0.000155562 0.000155562

5

164.9534

AT1G75750.1 92.4279 0.835658642 4.826211 1.39E-06 0.000166266 0.000166266

5

AT5G28050.2 22.4831 0 7.337228301 4.814964 1.47E-06 0.000175148 0.000175148

AT1G64720.1 380.8885 542.51 -4.807789 1.53E-06 0.000179994 0.000179994

0.510281001

23.95319

AT3G03341.1 1.69904 3.81742647 4.788494 1.68E-06 0.000197344 0.000197344

65

AT1G65490.1 89.1382 38.1739 1.223457243 4.768935 1.85E-06 0.000216577 0.000215758

AT2G05520.3 118.6263 58.7657 1.013377655 4.764567 1.89E-06 0.000219453 0.000219453

AT3G23080.2 22.4045 1.21997 4.198870954 4.7624 1.91E-06 0.000220892 0.000220892

AT1G13440.2 401.5785 289.256 0.473335146 4.739875 2.14E-06 0.000244868 0.000244868

199.2271

AT4G23600.1 120.442 0.726075696 4.737148 2.17E-06 0.000247154 0.000247154

5

AT5G08280.1 267.697 401.27 -4.736139 2.18E-06 0.000247361 0.000247361

0.583972329

AT3G18080.1 97.6601 180.675 -4.729983 2.25E-06 0.000253932 0.000253932

0.887555734

AT4G02520.1 78.51135 31.5522 1.315160631 4.72219 2.33E-06 0.00026278 0.00026278

AT2G24200.1 88.28225 167.429 -4.7186 2.37E-06 0.000266367 0.000266367

0.923354131

AT2G42600.1 154.593 86.1563 0.843446796 4.706881 2.52E-06 0.000279857 0.000279857

AT3G05590.1 419.8245 305.998 0.456264138 4.702316 2.57E-06 0.000285034 0.000285034

AT3G04840.1 447.584 330.345 0.438184956 4.696787 2.64E-06 0.00029168 0.00029168

AT4G01080.1 137.5963 233.91 -4.695728 2.66E-06 0.000292023 0.000292023

0.765511864

AT2G42600.2 160.3205 90.7009 0.821770142 4.694648 2.67E-06 0.000292401 0.000292401

AT5G49030.2 0 21.2129 -4.690047 2.73E-06 0.000297867 0.000297867

6.799703959

AT2G42220.1 398.643 559.451 -4.67278 2.97E-06 0.000322765 0.000321863

0.488914441

AT4G27520.1 477.796 357.505 0.418431357 4.672545 2.97E-06 0.000321863 0.000321863

21.93812

AT1G70410.1 1.33091 4.042955142 4.66398 3.10E-06 0.000334246 0.000334246

25

AT1G20620.1 119.0748 208.498 -4.663085 3.12E-06 0.000334393 0.000334393

0.808165419

AT4G37910.1 98.44825 45.6804 1.107790278 4.648985 3.34E-06 0.00035669 0.00035669

AT5G13510.1 423.24 588.134 -4.646971 3.37E-06 0.000358791 0.000358791

0.474668913

20.07786

AT2G03680.2 0 6.73495905 4.638918 3.50E-06 0.000371618 0.000371618

9

117.2258

AT2G05520.4 59.2447 0.984532738 4.631015 3.64E-06 0.000384605 0.000384605

5 117.2599

AT2G05520.6 59.3782 0.981705082 4.621297 3.81E-06 0.000401526 0.000401163

5

34.59604

AT3G57260.1 6.7224 2.363558825 4.620691 3.82E-06 0.000401163 0.000401163

5

AT1G20620.5 112.8033 198.852 -4.60457 4.13E-06 0.000430243 0.000430243

0.817885799

100.7092

AT5G09510.1 47.9177 1.071565632 4.583684 4.57E-06 0.000473767 0.000473767

5

19.33259

AT4G28140.1 0 6.455159995 4.58112 4.62E-06 0.000477811 0.000477811

4

114.2347

AT2G05520.5 57.9495 0.979133464 4.551952 5.32E-06 0.000547052 0.000547052

5

AT5G10760.1 34.946 7.18588 2.281890582 4.550837 5.34E-06 0.000547907 0.000547907

AT1G48300.1 55.4394 18.3987 1.591307811 4.547651 5.42E-06 0.000554197 0.000554197

AT1G66580.1 204.8385 127.991 0.678444532 4.546114 5.46E-06 0.000556191 0.000556191

129.7008

AT1G06430.1 69.7416 0.895096567 4.519763 6.19E-06 0.000627794 0.000627794

5

AT3G62870.1 430.363 319.92 0.427842863 4.5161 6.30E-06 0.000636396 0.000636396

AT4G16720.1 474.944 359.375 0.402267366 4.510565 6.47E-06 0.000650837 0.000650837

185.9050

AT4G23600.3 113.424 0.71284002 4.508321 6.53E-06 0.000655359 0.000655359

5

AT3G45970.1 31.7402 5.89237 2.429391289 4.494996 6.96E-06 0.000692714 0.000692714

AT5G58420.1 259.923 174.514 0.574741522 4.472116 7.74E-06 0.000765606 0.000765606

AT5G61820.1 63.1373 23.7803 1.408725651 4.452035 8.51E-06 0.000834836 0.000834836

AT3G09440.1 183.1945 112.324 0.705709973 4.439 9.04E-06 0.000880734 0.000880734

19.37297

AT4G30290.1 0 7.568540481 4.428378 9.49E-06 0.000921969 0.000921969

2

AT5G19240.1 59.28965 21.6634 1.452520593 4.410138 1.03E-05 0.000999621 0.000999621

AT1G56045.1 269.169 183.709 0.551089959 4.396776 1.10E-05 0.001059421 0.001059421

AT1G67870.1 369.085 269.725 0.452463859 4.378713 1.19E-05 0.001143118 0.001143118

39.74885

AT5G36700.3 91.3503 -4.37641 1.21E-05 0.001151244 0.001151244

5 1.200496164

17.59498

AT4G39320.1 0 5.011798359 4.367156 1.26E-05 0.001196942 0.001196942

5

AT1G21500.1 460.866 622.934 -4.362522 1.29E-05 0.001218372 0.001218372

0.434731979

AT2G45960.1 389.639 288.692 0.432606958 4.336259 1.45E-05 0.001363965 0.001363965

113.5348

AT1G04680.1 60.0109 0.919838735 4.320787 1.55E-05 0.001458219 0.001454106

5

221.8808

AT3G08580.1 332.816 -4.32024 1.56E-05 0.001456866 0.001454106

86 0.584939401

195.4151

AT1G52400.1 124.024 0.655922995 4.319909 1.56E-05 0.001454106 0.001454106

5

110.9747

AT1G19450.1 58.3038 0.928569638 4.303715 1.68E-05 0.001554089 0.001554089

5

AT3G53260.1 95.67 47.1698 1.020203114 4.301113 1.70E-05 0.001567177 0.001567177

AT1G04480.1 265.1155 182.525 0.53852694 4.281704 1.85E-05 0.001704627 0.001704627

AT2G36630.1 46.5118 14.7938 1.652604096 4.275287 1.91E-05 0.00174863 0.00174863

AT1G64510.1 386.603 531.557 -4.269728 1.96E-05 0.001786824 0.001786824

0.459371571

AT2G43100.1 268.03 388.305 -4.265824 1.99E-05 0.001812342 0.001812342

0.534795797

AT2G27530.1 310.8025 222.322 0.48334739 4.242049 2.21E-05 0.001989124 0.001989124

19.88279

AT2G23130.2 1.74141 3.513192758 4.237135 2.26E-05 0.002026522 0.002026522

5

AT5G09810.1 301.848 215.173 0.488325183 4.215827 2.49E-05 0.002220466 0.002213536

AT3G03150.1 139.3555 225.769 -4.215801 2.49E-05 0.002213536 0.002213536

0.696077462

AT4G19710.1 16.6726 0 4.65839719 4.204096 2.62E-05 0.00232372 0.00232372 AT1G18540.1 310.8365 223.222 0.477676701 4.201334 2.65E-05 0.002344694 0.002344694

AT4G08950.1 27.71085 5.18266 2.418686308 4.18963 2.79E-05 0.002461043 0.002461043

17.88963

AT2G02990.1 0 7.929592137 4.186811 2.83E-05 0.002483837 0.002483837

53

AT3G22840.1 29.33637 5.98508 2.293247931 4.181673 2.89E-05 0.002532552 0.002532552

AT3G61430.1 195.7595 126.546 0.629420416 4.180629 2.91E-05 0.002536138 0.002536138

AT1G64200.1 58.5824 116.181 -4.173988 2.99E-05 0.002594771 0.002594771

0.987834949

AT5G08640.1 34.404 8.62201 1.99648017 4.163756 3.13E-05 0.002705365 0.002705365

AT1G67865.1 238.648 162.579 0.553743326 4.156296 3.23E-05 0.002786423 0.002786423

AT1G78040.1 164.6155 101.804 0.693305937 4.147727 3.36E-05 0.002883719 0.002872322

AT5G59850.1 206.154 135.643 0.603907856 4.14749 3.36E-05 0.002877712 0.002872322

AT3G22120.1 206.7305 309.514 -4.147207 3.37E-05 0.002872322 0.002872322

0.582253415

AT4G13930.1 276.018 194.789 0.502850144 4.129655 3.63E-05 0.003072027 0.003072027

AT2G26080.1 244.7025 168.207 0.540791096 4.12772 3.66E-05 0.003088477 0.003088477

AT1G75690.1 429.7455 577.771 -0.42701526 -4.122477 3.75E-05 0.003140371 0.003140371

AT2G23120.1 94.4464 48.183 0.970971584 4.111925 3.92E-05 0.003277454 0.003277454

AT3G48460.1 79.9663 37.8417 1.079415235 4.107401 4.00E-05 0.003332161 0.003332161

AT3G23080.1 23.5216 62.2661 -1.40446072 -4.106152 4.02E-05 0.003340108 0.003340108

AT1G26770.2 52.84815 19.8332 1.413935501 4.083991 4.43E-05 0.003664308 0.003664308

AT5G39740.1 361.4705 270.102 0.42037359 4.079369 4.52E-05 0.003726694 0.003726694

AT1G01550.1 59.3871 24.116 1.30015895 4.072412 4.65E-05 0.00382832 0.00382832

AT5G60790.1 88.68805 44.4266 0.997315991 4.068755 4.73E-05 0.003865747 0.003865747

AT1G56110.1 71.9269 32.6163 1.140938295 4.064023 4.82E-05 0.003933297 0.003933297

AT5G50915.1 46.7127 16.1821 1.529415993 4.060338 4.90E-05 0.003984065 0.003984065

AT4G26850.1 248.3955 172.655 0.524746923 4.057153 4.97E-05 0.004026862 0.004026862

AT2G45960.3 312.0555 227.472 0.456113668 4.052782 5.06E-05 0.004090799 0.004090799

AT2G45660.1 21.37576 2.84639 2.908770463 4.051007 5.10E-05 0.004109877 0.004109877

AT1G29660.1 329.3605 242.616 0.440992848 4.049968 5.12E-05 0.004116092 0.004116092

14.86674

AT3G22620.1 0 5.587465388 4.046361 5.20E-05 0.004167813 0.004167813

55

19.01078

AT5G22500.1 1.96224 3.276244617 4.029802 5.58E-05 0.004459573 0.004459573

4

AT2G45560.2 0 16.4005 -4.024495 5.71E-05 0.004534986 0.004510541

4.384561539

AT4G09010.1 321.969 446.542 -4.024143 5.72E-05 0.004528668 0.004510541

0.471874087

AT2G21970.1 77.8651 37.1049 1.069367122 4.024029 5.72E-05 0.004517856 0.004510541

AT2G38750.1 65.0826 122.903 -0.91717634 -4.018387 5.86E-05 0.004600932 0.004600932

14.96870

AT1G54575.1 0 4.819717975 4.006379 6.17E-05 0.004827325 0.004827325

5

AT5G28060.1 318.717 234.408 0.443254165 4.000738 6.31E-05 0.004929815 0.004929815

AT1G54100.2 19.33045 2.22785 3.117151218 3.977359 6.97E-05 0.005409646 0.005409646

AT5G02450.1 255.9535 180.71 0.502205391 3.972496 7.11E-05 0.005505764 0.005505764

AT4G15440.1 60.80035 116.117 -0.93342767 -3.967902 7.25E-05 0.00559718 0.00559718

AT1G64770.1 162.779 103.012 0.660102182 3.963107 7.40E-05 0.005694842 0.005694842

AT3G51920.1 126.989 74.6499 0.766491301 3.951004 7.78E-05 0.005957332 0.005957332

AT3G05220.2 0 15.5797 -3.944393 8.00E-05 0.006090159 0.006090159

4.486207627 AT5G22620.2 18.9079 2.18907 3.110599146 3.92997 8.50E-05 0.006431659 0.006431659

154.7682

AT1G52400.2 97.0833 0.672814486 3.924834 8.68E-05 0.006552365 0.006552365

5

AT5G13650.1 175.642 114.192 0.621176285 3.917524 8.95E-05 0.006717307 0.006717307

AT4G25700.1 64.34235 28.6802 1.165713544 3.906901 9.35E-05 0.007000353 0.007000353

29.09972

AT5G54710.1 6.99806 2.05597858 3.900238 9.61E-05 0.007156852 0.007156852

5

AT4G34670.1 253.273 179.847 0.493923193 3.897996 9.70E-05 0.007203877 0.007203877

AT3G53020.1 265.962 191.009 0.477579515 3.885518 0.0001021 0.007563726 0.007563726

AT2G32240.1 36.5203 11.1272 1.714608012 3.884315 0.0001026 0.007580824 0.007580824

AT4G01310.1 381.159 512.331 -3.878677 0.000105 0.007737848 0.007737848

0.426683247

19.47636

AT2G44120.2 52.9524 -3.868399 0.0001096 0.008028299 0.008028299

5 1.442971631

AT1G76160.1 133.5865 81.0125 0.721557785 3.859374 0.0001137 0.008308461 0.008308461

AT5G58770.1 28.1426 6.77607 2.054234937 3.833542 0.0001263 0.009207334 0.009207334

AT5G13650.2 186.3285 124.418 0.582653139 3.828971 0.0001287 0.009355199 0.009355199

AT4G20830.2 33.78515 9.86844 1.775495313 3.824345 0.0001311 0.009507427 0.009507427

AT5G10360.1 310.636 231.288 0.425534619 3.819607 0.0001337 0.009666418 0.009666418

AT1G68520.1 87.17055 45.762 0.929690707 3.817901 0.0001346 0.009707912 0.009707912

AT5G24490.1 97.3623 163.033 -0.74372886 -3.815122 0.0001361 0.00979214 0.00979214

102.1920

AT1G04430.1 57.1517 0.838414649 3.808586 0.0001398 0.010028307 0.010008044

5

35.66851

AT5G65390.1 11.0193 1.694618573 3.808442 0.0001398 0.010008044 0.010008044

5

25.43309

AT3G01290.1 5.5356 2.199895434 3.79943 0.000145 0.010352058 0.01034748

5

AT3G21670.1 97.6897 53.9061 0.857757921 3.792046 0.0001494 0.010609746 0.010609746

AT5G15350.1 177.596 117.931 0.590656085 3.780168 0.0001567 0.011071722 0.011065706

AT1G69420.2 14.7296 0 3.894474273 3.779026 0.0001574 0.011065706 0.011065706

AT5G13710.2 3.81119 22.6792 -3.770367 0.000163 0.011427593 0.011427593

2.573056313

AT4G09000.1 138.2455 86.0323 0.684282208 3.760731 0.0001694 0.01184671 0.01184671

AT2G20450.1 132.7545 81.6483 0.701266012 3.759396 0.0001703 0.011879908 0.011879908

AT5G05270.1 33.90255 10.2961 1.71929582 3.749404 0.0001773 0.012269894 0.012269894

AT2G23090.1 314.92 236.711 0.411858633 3.744207 0.000181 0.012495173 0.012495173

AT4G12600.1 101.0628 57.102 0.823638874 3.734066 0.0001884 0.012912178 0.012912178

AT3G04920.1 200.002 137.637 0.539146075 3.72231 0.0001974 0.013461488 0.013461488

AT1G78680.1 76.57135 133.718 -3.717764 0.000201 0.013638325 0.013601946

0.804317084

23.57028

AT2G30790.1 57.9478 -3.717226 0.0002014 0.013633717 0.013601946

5 1.297784687

AT4G25700.2 0 14.093 -3.717194 0.0002014 0.013601946 0.013601946

4.323401946

AT5G06320.1 57.51165 25.494 1.17369648 3.7128 0.000205 0.013806441 0.013802516

AT3G02230.1 96.5691 53.9015 0.84123621 3.712252 0.0002054 0.013802516 0.013802516

AT4G13940.4 127.384 78.0935 0.705909702 3.702331 0.0002136 0.014283971 0.014283971

104.1147

AT5G67370.1 59.8091 0.799737557 3.701688 0.0002142 0.014285465 0.014285465

5

AT1G09590.1 248.8525 179.988 0.467390157 3.692406 0.0002221 0.014745624 0.014745624

AT5G13490.1 30.75745 8.79014 1.806977851 3.691681 0.0002228 0.014752143 0.014752143

AT3G44860.1 52.76995 100.632 -3.686303 0.0002275 0.014994962 0.014994962

0.931300619 AT1G01550.2 3.815575 22.074 -3.685626 0.0002281 0.014998893 0.014998893

2.532375692

AT5G47560.1 65.289 31.4222 1.055055751 3.646683 0.0002656 0.017381555 0.017381555

AT4G 14040.1 250.1965 349.582 -3.643955 0.0002685 0.017483656 0.017483656

0.482569295

112.8117

AT1G09780.1 67.2892 0.745470466 3.641317 0.0002712 0.017622061 0.017622061

5

AT3G44750.1 52.5095 22.627 1.214533141 3.640163 0.0002725 0.017659525 0.017659525

AT5G08610.1 54.60045 24.2421 1.171398162 3.612252 0.0003035 0.019581802 0.019581802

12.14895

AT2G37770.2 0 4.838740053 3.611525 0.0003044 0.019590797 0.019590797

25

AT1G29395.1 38.73728 13.9761 1.47076082 3.596588 0.0003224 0.020701855 0.020701855

AT3G12965.1 75.92265 39.5693 0.940148805 3.594564 0.0003249 0.020814823 0.020814823

AT3G48930.1 293.242 220.976 0.408202062 3.586692 0.0003349 0.021403209 0.021403209

AT5G56030.1 129.9964 81.7603 0.668999276 3.580396 0.0003431 0.021874742 0.021874742

AT1G03870.1 100.0647 58.0459 0.785667049 3.577617 0.0003467 0.022057384 0.022057384

AT5G61960.2 13.8915 1.16435 3.576605691 3.565297 0.0003634 0.023066583 0.023066583

AT1G23490.1 267.5665 198.841 0.428282234 3.563631 0.0003658 0.023160003 0.023114921

AT1G32080.1 274.819 376.858 -3.563539 0.0003659 0.023114921 0.023114921

0.455539268

AT2G37190.1 206.018 145.56 0.501156436 3.557777 0.000374 0.023573553 0.023573553

AT2G28190.1 76.8872 40.6387 0.91988919 3.555801 0.0003768 0.023697171 0.023697171

113.6606

AT4G13940.2 68.9889 0.720296708 3.554853 0.0003782 0.023728461 0.023728461

5

AT1G43710.1 67.8649 34.1304 0.991608277 3.543276 0.0003952 0.024626281 0.024626281

AT1G26770.1 38.86326 14.358 1.436552122 3.542353 0.0003966 0.02465671 0.02465671

AT3G56360.1 52.46815 23.3549 1.167716654 3.532595 0.0004115 0.025527071 0.025527071

12.90172

AT2G47485.1 0 3.836012242 3.519845 0.0004318 0.026725832 0.026725832

6

AT2G41090.1 216.6435 305.831 -3.516221 0.0004377 0.027032407 0.026989544

0.497411698

AT4G21620.1 69.4058 35.4847 0.967859119 3.516047 0.000438 0.026989544 0.026989544

AT2G43290.1 33.3801 11.163 1.58026348 3.511705 0.0004452 0.027372866 0.027372866

AT2G20570.1 147.0465 96.6855 0.604900997 3.507429 0.0004525 0.027754539 0.027754539

AT3G19030.1 43.476 17.5363 1.309874827 3.503563 0.0004591 0.028097865 0.028097865

AT5G03240.3 19.96325 49.9114 -3.502132 0.0004616 0.028123916 0.028123916

1.322022762

AT2G27420.1 23.75605 5.7992 2.034369175 3.500909 0.0004637 0.028190767 0.028190767

AT3G07050.1 43.253 17.4534 1.309292093 3.493421 0.0004769 0.028929296 0.028929296

AT1G61570.1 64.44595 32.1158 1.004806463 3.488613 0.0004855 0.029389781 0.029389781

AT2G39030.1 47.57119 20.3481 1.225194026 3.487512 0.0004875 0.029446156 0.029446156

121.0834

AT1G54270.1 75.7629 0.676438228 3.486697 0.000489 0.029471303 0.029471303

5

AT2G34620.1 78.36245 132.249 -3.485199 0.0004918 0.029507306 0.029507306

0.755022404

11.35781

AT5G44420.1 0 4.697724965 3.475127 0.0005106 0.030504632 0.030504632

96

AT5G14200.1 132.0217 200.827 -3.470602 0.0005193 0.030888921 0.030888921

0.605178164

AT3G13920.3 150.2965 100.002 0.58778256 3.463605 0.000533 0.031635067 0.031635067

12.37697

AT3G50970.1 0 3.885002168 3.461468 0.0005372 0.031818619 0.031818619

6

AT5G08760.1 42.9918 17.4898 1.297547723 3.459807 0.0005406 0.03187816 0.031858932

AT1G64500.1 62.9123 31.2495 1.009509 3.459392 0.0005414 0.031858932 0.031858932 AT3G50685.1 227.1525 317.031 -3.458168 0.0005439 0.031867489 0.031867489

0.480962734

AT5G37770.1 31.36805 10.2605 1.612194811 3.451731 0.000557 0.032568092 0.032568092

AT3G01820.1 38.8337 14.8564 1.386224606 3.45112 0.0005583 0.032572568 0.032572568

AT5G64840.1 181.511 126.477 0.521181929 3.448696 0.0005633 0.032796734 0.032796734

25.20068

AT3G18410.2 57.4904 -3.446038 0.0005689 0.033050965 0.033050965

5 1.189858119

AT2G43570.1 12.61471 0 3.700068534 3.438789 0.0005843 0.033877117 0.033877117

AT3G20390.1 259.4635 355.003 -3.432903 0.0005972 0.034548334 0.034538772

0.452299614

AT5G52470.1 82.98185 46.4496 0.837129648 3.427769 0.0006086 0.035060602 0.035013801

AT2G45960.2 210.535 151.72 0.472648815 3.427563 0.000609 0.035013801 0.035013801

AT1G69870.1 29.4477 9.28845 1.664645184 3.418698 0.0006292 0.036099114 0.036099114

AT5G16130.1 270.425 204.381 0.403967446 3.414987 0.0006379 0.036518284 0.036518284

AT5G38120.1 6.892615 26.4538 -3.404284 0.0006634 0.037822197 0.037822197

1.940351637

AT4G37540.1 41.8206 17.1081 1.289534215 3.39678 0.0006818 0.038794186 0.038794186

AT1G09240.1 19.73373 4.14063 2.252741478 3.394118 0.0006885 0.039092243 0.039092243

AT4G25630.1 46.8327 20.5187 1.190576885 3.386681 0.0007074 0.040084644 0.040084644

AT5G24120.1 33.2371 11.7192 1.503920421 3.384597 0.0007128 0.040307074 0.040307074

AT1G04040.1 267.1705 202.3 0.4012644 3.375756 0.0007361 0.04153929 0.04153929

AT2G39390.1 114.5075 71.8784 0.671811895 3.371804 0.0007468 0.042053534 0.042053534

AT1G29250.1 59.2979 29.3775 1.013269384 3.368264 0.0007564 0.042510191 0.042477404

AT1G70780.1 75.82905 41.596 0.866305848 3.367351 0.0007589 0.042520705 0.042477404

AT1G70782.1 75.82905 41.596 0.866305848 3.367351 0.0007589 0.042520705 0.042477404

11.79340

AT1G43160.1 0 8.128882501 3.366021 0.0007626 0.042596051 0.042530922

55

AT3G13120.1 276.9475 373.91 -0.43307854 -3.365883 0.000763 0.042530922 0.042530922

AT2G15020.1 34.35115 12.5067 1.457657226 3.365308 0.0007646 0.042533379 0.042533379

AT1G72430.1 83.0429 47.1253 0.817355023 3.364135 0.0007678 0.042628363 0.042628363

AT3G03920.1 68.9142 36.4634 0.918352195 3.361944 0.000774 0.042881392 0.042881392

AT5G28840.1 127.2875 82.4102 0.627195932 3.361269 0.0007759 0.042899783 0.042899783

AT5G62190.1 64.5973 33.3699 0.952926502 3.350971 0.0008053 0.044348959 0.044272139

AT3G48000.1 164.4945 113.739 0.532312322 3.340521 0.0008362 0.045868282 0.045868282

AT2G16660.1 179.909 126.875 0.503859537 3.339389 0.0008396 0.045963774 0.045963774

AT4G 14746.1 15.39521 2.31579 2.732905209 3.333651 0.0008571 0.046829176 0.046829176

AT5G05600.1 38.43492 15.3466 1.3244986 3.321168 0.0008964 0.048877742 0.048869207

AT5G10380.1 24.9028 7.12153 1.806048834 3.320664 0.000898 0.048869207 0.048869207

AT3G16080.1 193.064 138.572 0.4784434 3.315274 0.0009155 0.04972293 0.04972293

10.53352

AT4G21105.2 0 4.470537721 3.314468 0.0009182 0.049768117 0.049768117

75

C24xCol at ZT6

MPV C24xCol log2(Fold_c

Gene Name z-score p-value q-value q-value

RPKM RPKM hange)

AT2G25510.1 611.3505 3869.93 -51.38346 0 0 0

2.662235821

AT5G42530.1 936.878 3577.68 -40.76095 0 0 0

1.933091256

315.6361

AT1G31580.1 1651.36 -31.56433 1.14E-218 1.0576E-214 1.0576E-214

28 2.387320413 AT5G38430.1 9310.055 5752.33 0.694643248 28.89545 1.36E-183 7.5793E-180 7.5793E-180

AT3G22231.1 178.0048 660.3000 -28.35438 7.39E-177 3.428E-173 3.428E-173

2.704509818

AT5G38420.1 7993.535 4929.52 0.697386476 26.85234 7.92E-159 3.1482E-155 3.1482E-155

AT3G15353.1 1288.045 2949.59 -26.07815 6.45E-150 2.2439E-146 2.2439E-146

1.195331433

AT2G34420.1 8960.135 12674.1 -25.90238 6.26E-148 1.9355E-144 1.9355E-144

0.500290929

AT1G72610.1 3176.92 1474.8 1.10710944 25.08491 7.27E-139 2.022E-135 2.022E-135

AT5G38410.1 7088.105 4653.98 0.606934965 22.30605 3.23E-110 8.1642E-107 8.1642E-107

AT5G17920.1 1161.278 375.489 1.628870833 20.417 1.18E-92 2.73849E-89 2.73849E-89

1315.334

AT5G11740.1 473.186 1.47495044 20.19821 1.02E-90 2.17287E-87 2.17287E-87

5

AT5G17920.2 13.88325 382.905 -20.06434 1.51E-89 3.00695E-86 3.00695E-86

4.785569259

AT4G25100.4 357.812 5.34104 6.065937273 19.63069 8.46E-86 1.56858E-82 1.56858E-82

AT2G10940.1 1218.745 466.415 1.385710208 18.54352 9.20E-77 1.59999E-73 1.59999E-73

AT1G66100.1 679.961 1526.48 -18.39503 1.44E-75 2.35683E-72 2.35683E-72

1.166684781

458.2150

AT2G42540.2 63.9289 2.906206901 -18.37543 2.07E-75 3.19483E-72 3.19483E-72

5

AT2G41090.1 216.6435 773.524 -18.29638 8.84E-75 1.29509E-71 1.29509E-71

1.836123103

441.3380

AT2G42540.1 61.8759 2.834434625 17.80726 6.21E-71 8.63563E-68 8.63563E-68

5

AT1G76960.1 9.92065 254.398 -16.28972 1.17E-59 1.41258E-56 1.41258E-56

4.680508869

19.60383

AT1G14880.1 281.693 -16.28168 1.33E-59 1.54379E-56 1.54379E-56

5 3.844915899

AT3G22235.1 12.89104 258.424 -16.13781 1.38E-58 1.53999E-55 1.53999E-55

4.325299496

AT5G38410.2 4347.715 2955.89 0.55666479 16.12193 1.79E-58 1.91485E-55 1.91485E-55

AT3G22235.2 28.66485 292.907 -15.77219 4.83E-56 4.80427E-53 4.80427E-53

3.353088037

AT2G36830.1 703.7615 233.299 1.592906469 15.63618 4.13E-55 3.82821E-52 3.82821E-52

AT5G38410.3 4226.515 2893.7 0.54655321 15.629 4.62E-55 4.14666E-52 4.14666E-52

AT2G10940.2 874.739 337.023 1.376005567 15.62358 5.03E-55 4.37375E-52 4.37375E-52

AT1G64370.1 211.228 634.748 -14.97024 1.15E-50 9.68816E-48 9.68816E-48

1.587382856

AT1G19960.1 18.65192 241.06 -14.85232 6.72E-50 5.50009E-47 5.50009E-47

3.691996227

AT1G75040.1 97.9543 16.7790 2.082162123 14.5494 5.89E^8 4.68366E-45 4.68366E-45

AT3G23810.1 566.5985 180.975 1.646536366 14.36992 7.99E-17 6.17718E-44 6.17718E-44

AT4G21960.1 466.8715 991.922 -14.01437 1.27E^4 9.32259E-42 9.32259E-42

1.087201156

AT5G03350.1 23.60015 219.943 -13.43274 3.89E^1 2.77366E-38 2.77366E-38

3.220261752

AT3G17390.1 666.1755 265.899 1.325023945 13.24164 5.04E-W 3.50858E-37 3.50858E-37

AT5G42980.1 762.189 1355.23 -13.11378 2.75E-39 1.86338E-36 1.86338E-36

0.830317024

346.5066

AT5G59320.1 88.2156 1.973777331 12.73814 3.63E-37 2.40446E-34 2.40446E-34

5

AT5G10760.1 34.946 229.594 -12.63815 1.30E-36 8.41719E-34 8.41719E-34

2.715885704

AT1G23130.1 536.9025 1004.57 -12.12274 8.00E-34 4.94913E-31 4.94913E-31

0.903846069

134.4629

AT5G59310.1 5.20005 4.692538897 11.80582 3.64E-32 2.20313E-29 2.20313E-29

03

113.8939

AT4G19170.1 363.427 -11.76324 6.04E-32 3.57416E-29 3.57416E-29

5 1.673974492

AT3G44860.1 52.76995 244.965 -11.61978 3.27E-31 1.89548E-28 1.89548E-28

2.214787114 AT4G16190.1 127.3365 378.674 -11.4825 1.62E-30 9.17288E-28 9.17288E-28

1.572310353

AT4G02520.1 78.51135 291.53 -11.46206 2.05E-30 1.1386E-27 1.1386E-27

1.892671215

AT3G14420.4 37.59345 206.054 -11.32547 9.81E-30 5.35471E-27 5.35471E-27

2.454469245

AT3G16240.1 757.2185 380.334 0.993442744 11.18741 4.70E-29 2.51465E-26 2.51465E-26

AT3G26520.1 1303.235 789.03 0.723945195 11.17399 5.47E-29 2.87018E-26 2.87018E-26

AT2G45180.1 1598.02 1027.31 0.637413871 11.04301 2.37E-28 1.22099E-25 1.22099E-25

AT4G16980.1 814.2085 434.427 0.906284512 10.73555 6.93E-27 3.44345E-24 3.44345E-24

AT5G19140.1 93.59195 293.191 -10.4464 1.52E-25 7.42975E-23 7.42975E-23

1.647384467

AT3G08580.2 650.6945 326.505 0.994875274 10.38273 2.97E-25 1.42551E-22 1.40316E-22

AT3G13520.1 355.206 129.617 1.454401003 10.38261 2.98E-25 1.40316E-22 1.40316E-22

105.2391

AT2G38530.1 4.6991 4.485143084 10.3472 4.31E-25 1.99832E-22 1.99832E-22

4

AT1G67870.1 369.085 697.978 -10.24655 1.23E-24 5.50392E-22 5.41663E-22

0.919228458

AT1G19670.1 164.022 402.608 -10.24655 1.23E-24 5.41663E-22 5.41663E-22

1.295486505

AT5G42650.1 434.275 783.331 -0.85101315 -10.17682 2.52E-24 1.09407E-21 1.09407E-21

ATCG01210.1 348.6355 131.691 1.404562728 10.01774 1.27E-23 5.45288E-21 5.45288E-21

AT3G26740.1 99.45095 284.496 -9.69365 3.21E-22 1.35268E-19 1.35268E-19

1.516351311

AT1G15930.2 282.746 101.639 1.476052528 9.366527 7.50E-21 3.113E-18 3.113E-18

AT5G13930.1 427.8275 195.266 1.131588452 9.360389 7.94E-21 3.25076E-18 3.25076E-18

AT4G00430.1 221.075 66.0414 1.74309328 9.34636 9.07E-21 3.65824E-18 3.65824E-18

AT1G24147.1 19.4416 125.673 -2.69245587 -9.307352 1.31E-20 5.20959E-18 5.20959E-18

AT1G45145.1 32.44625 154.024 -9.29514 1.47E-20 5.76142E-18 5.76142E-18

2.247031517

AT2G16600.2 238.351 478.887 -9.146274 5.89E-20 2.24624E-17 2.24624E-17

1.006597594

AT1G04040.1 267.1705 96.8646 1.463719298 9.047644 1.46E-19 5.49303E-17 5.49303E-17

104.0749

AT1G66970.1 270.343 -8.796531 1.41E-18 5.23518E-16 5.23518E-16

5 1.377168136

AT5G37600.1 46.7268 169.984 -8.660092 4.71E-18 1.7034E-15 1.7034E-15

1.863076812

AT3G09820.1 214.831 71.0585 1.596123049 8.650346 5.13E-18 1.83159E-15 1.83159E-15

ATCGOl 180.1 739.6015 443.431 0.738038519 8.560397 1.12E-17 3.96169E-15 3.96169E-15

102.5731

AT3G25760.1 260.361 -8.473838 2.37E-17 8.15641E-15 8.15641E-15

5 1.343860226

10.15764

AT1G15930.1 87.772 -8.355275 6.53E-17 2.18848E-14 2.18848E-14

5 3.111194822

AT1G64360.1 9.670385 86.4521 -8.351314 6.75E-17 2.23618E-14 2.23618E-14

3.160255776

AT2G43570.1 12.61471 93.833 -8.347189 6.99E-17 2.28842E-14 2.28842E-14

2.894988351

AT3G60245.1 766.863 471.311 0.70228951 8.341281 7.35E-17 2.37775E-14 2.37775E-14

62.71857

AT2G14610.1 3.0800 3.999400127 7.954437 1.51E-16 1.05186E-13 1.05186E-13

3

AT5G10380.1 24.9028 119.302 -2.26023836 -8.209622 2.22E-16 7.01588E-14 7.01588E-14

AT4G23670.1 600.8025 914.259 -8.198415 2.44E-16 7.61524E-14 7.61524E-14

0.605712108

AT4G32940.1 20.4915 109.229 -8.167735 3.14E-16 9.71503E-14 9.71503E-14

2.414258439

AT5G19140.2 59.93795 183.233 -8.131782 4.23E-16 1.29349E-13 1.29349E-13

1.612137707

109.4150

AT2G46600.1 261.635 -8.073213 6.85E-16 2.07091E-13 2.0709 IE- 13

5 1.257744355 AT3G28270.2 285.9618 509.557 -8.06356 7.41E-16 2.19355E-13 2.19355E-13

0.833421099

88.25640

AT4G16590.1 11.8691 2.894490463 8.047552 8.45E-16 2.47399E-13 2.47399E-13

65

AT4G26530.1 114.9041 268.26 -7.999542 1.25E-15 3.58233E-13 3.58233E-13

1.223201675

109.3148

AT5G52310.1 21.5452 2.343051135 7.971586 1.57E-15 4.44775E-13 4.44775E-13

6

AT1G72930.1 92.90955 231.688 -7.877205 3.35E-15 9.40949E-13 9.40949E-13

1.318284522

AT4G26530.2 109.9663 257.106 -7.841935 4.44E-15 1.23447E-12 1.23447E-12

1.225301812

AT1G27950.1 270.783 118.057 1.197653582 7.794587 6.46E-15 1.76278E-12 1.76278E-12

AT1G55330.1 447.538 249.397 0.843566074 7.483005 7.26E-14 1.92502E-11 1.92502E-11

AT5G44020.1 296.737 140.968 1.073817122 7.468575 8.11E-14 2.12804E-11 2.12804E-11

AT3G25520.1 515.7785 302.737 0.768686627 7.408762 1.27E-13 3.31518E-11 3.31518E-11

AT3G09390.1 205.567 381.015 -7.369934 1.71E-13 4.3982E-11 4.3982E-11

0.890239111

AT1G70890.1 39.63525 132.839 -7.320318 2.47E-13 6.31509E-11 6.31509E-11

1.744822786

110.2576

AT5G04140.2 244.968 -7.290384 3.09E-13 7.81812E-11 7.81812E-11

75 1.151714218

AT3G62410.1 540.7345 801.816 -0.5683508 -7.257147 3.95E-13 9.91024E-11 9.91024E-11

AT2G06850.1 130.2085 38.1651 1.770497754 7.251446 4.12E-13 1.02442E-10 1.02364E-10

AT2G05520.2 126.8635 268.291 -7.250345 4.16E-13 1.02364E-10 1.02364E-10

1.080521609

AT1G67430.1 550.251 334.503 0.718070712 7.205929 5.76E-13 1.4071E-10 1.4071E-10

AT4G30530.1 312.5785 515.58 -7.174667 7.25E-13 1.73867E-10 1.72458E-10

0.721977752

AT4G39090.1 85.48125 205.208 -7.174606 7.25E-13 1.72458E-10 1.72458E-10

1.263407065

AT1G29660.1 329.3605 169.712 0.956578965 7.146397 8.91E-13 2.10067E-10 2.10067E-10

AT1G59870.1 55.5652 156.899 -7.133028 9.82E-13 2.29579E-10 2.29579E-10

1.497582634

AT1G49750.1 93.0316 214.596 -7.07502 1.49E-12 3.46485E-10 3.46485E-10

1.205830441

AT3G25770.1 473.1745 710.846 -7.032668 2.03E-12 4.65945E-10 4.65945E-10

0.587164717

AT1G35710.1 6.546205 59.6782 -6.966254 3.25E-12 7.42359E-10 7.42359E-10

3.188473335

AT5G15200.1 652.0425 421.935 0.627945236 6.956112 3.50E-12 7.91298E-10 7.91298E-10

AT1G20440.1 160.9686 59.3688 1.439002433 6.932923 4.12E-12 9.17632E-10 9.17632E-10

AT5G44130.1 167.5135 63.4465 1.400664882 6.928828 4.24E-12 9.3709E-10 9.3709E-10

AT4G26850.1 248.3955 424.214 -6.894517 5.40E-12 1.18417E-09 1.18417E-09

0.772153196

AT1G72930.2 71.21175 175.961 -6.811872 9.63E-12 2.07796E-09 2.07796E-09

1.305068493

AT4G35100.1 375.694 210.823 0.833525807 6.785275 1.16E-11 2.47997E-09 2.47997E-09

AT5G20290.1 711.159 475.889 0.579547044 6.753689 1.44E-11 3.06143E-09 3.06143E-09

AT1G75380.2 21.78725 90.6164 -6.745885 1.52E-11 3.18202E-09 3.18202E-09

2.056288067

AT3G49910.1 427.8035 251.332 0.767353917 6.736858 1.62E-11 3.36077E-09 3.36077E-09

44.84697

AT1G64770.2 0 7.390903552 6.73223 1.67E-11 3.44376E-09 3.44376E-09

15

AT1G20620.2 53.6408 146.453 -6.725662 1.75E-11 3.57626E-09 3.56885E-09

1.449035087

AT1G75380.3 21.26615 89.2931 -6.724897 1.76E-11 3.56885E-09 3.56885E-09

2.069989824

324.0262

AT5G35630.2 515.312 -6.716873 1.86E-11 3.74358E-09 3.74358E-09

65 0.669335429

AT4G00430.2 14.59439 74.9079 -6.674323 2.48E-11 4.97197E-09 4.97197E-09

2.359703965 AT1G10960.1 582.582 374.521 0.637414512 6.664507 2.66E-11 5.27792E-09 5.24315E-09

AT2G05520.3 118.6263 243.064 -6.664432 2.66E-11 5.24315E-09 5.24315E-09

1.034912335

AT4G25050.1 800.4635 552.684 0.534380772 6.650707 2.92E-11 5.71566E-09 5.71566E-09

AT3G62550.1 26.63585 98.2075 -6.628234 3.40E-11 6.6104E-09 6.6104E-09

1.882463885

AT3G56240.1 205.623 360.653 -6.625735 3.46E-11 6.67655E-09 6.67655E-09

0.810609778

AT3G18740.1 342.354 189.926 0.850051451 6.587948 4.46E-11 8.49894E-09 8.49894E-09

AT3G47370.3 0 40.0957 -6.582222 4.63E-11 8.77279E-09 8.76076E-09

5.367582087

AT3G47370.1 136.2025 48.1329 1.500657931 6.581418 4.66E-11 8.76076E-09 8.76076E-09

AT1G70700.1 114.4384 234.857 -6.562752 5.28E-11 9.86455E-09 9.86455E-09

1.037211362

AT1G70600.1 539.2125 342.844 0.653301667 6.555382 5.55E-11 1.02952E-08 1.02952E-08

AT2G43530.1 77.90625 181.223 -1.21795509 -6.5527 5.65E-11 1.04125E-08 1.04125E-08

AT5G64770.1 54.4497 144.355 -6.530836 6.54E-11 1.19727E-08 1.19727E-08

1.406625074

AT3G49010.3 151.2035 58.1052 1.379752349 6.5075 7.64E-11 1.38964E-08 1.38964E-08

AT5G23010.1 365.5995 559.938 -6.501496 7.95E-11 1.43688E-08 1.43688E-08

0.615002994

AT1G47128.1 180.017 323.387 -0.84512853 -6.499394 8.06E-11 1.4477E-08 1.4477E-08

AT3G45860.1 4.81574 49.5845 -6.497857 8.15E-11 1.45318E-08 1.45318E-08

3.364059799

AT2G21970.1 77.8651 179.753 -6.479923 9.18E-11 1.62642E-08 1.62642E-08

1.206967102

AT1G64720.1 380.8885 576.461 -6.434425 1.24E-10 2.1827E-08 2.1827E-08

0.597854277

AT5G57560.1 50.4339 5.29832 3.250786906 6.425242 1.32E-10 2.28964E-08 2.28964E-08

AT3G09820.2 0 43.7015 -6.405567 1.50E-10 2.58914E-08 2.58914E-08

8.251172061

AT4G38740.1 416.8195 618.579 -6.385419 1.71E-10 2.91784E-08 2.91784E-08

0.569535084

AT3G48930.1 293.242 158.085 0.891391267 6.350816 2.14E-10 3.61177E-08 3.61177E-08

AT5G60360.1 73.40985 170.558 -6.349839 2.16E-10 3.61289E-08 3.61289E-08

1.216216867

AT2G 18020.1 642.202 431.994 0.572015883 6.341342 2.28E-10 3.79508E-08 3.79508E-08

AT1G75380.1 18.7841 78.9449 -6.325823 2.52E-10 4.17186E-08 4.17186E-08

2.071334074

AT5G08760.1 42.9918 122.045 -6.314707 2.71E-10 4.45651E-08 4.45472E-08

1.505279771

AT2G32060.2 116.0795 38.8043 1.580824775 6.313856 2.72E-10 4.45472E-08 4.45472E-08

AT5G58420.1 259.923 134.729 0.94802388 6.300407 2.97E-10 4.83057E-08 4.83057E-08

AT2G41840.1 393.4945 234.956 0.743952861 6.2872 3.23E-10 5.22922E-08 5.22922E-08

AT3G28540.2 4.25398 45.6963 -3.4251922 -6.284034 3.30E-10 5.30604E-08 5.30604E-08

AT1G26770.2 52.84815 6.86553 2.944409968 6.280483 3.38E-10 5.39746E-08 5.39746E-08

AT3G28540.1 3.848515 44.483 -6.274903 3.50E-10 5.56264E-08 5.56264E-08

3.530880313

AT1G73600.2 83.0039 183.364 -6.27137 3.58E-10 5.65804E-08 5.65804E-08

1.143459392

AT5G41700.2 52.538 136.808 -6.2695 3.62E-10 5.69404E-08 5.69404E-08

1.380719409

AT4G17390.1 340.614 195.191 0.803251201 6.256316 3.94E-10 6.16178E-08 6.16178E-08

AT2G 14890.1 140.3554 54.2973 1.370132206 6.236095 4.49E-10 6.97379E-08 6.97379E-08

AT4G05320.3 217.646 365.354 -6.221302 4.93E-10 7.62167E-08 7.62167E-08

0.747311497

AT4G18100.1 465.2135 292.884 0.667563581 6.208057 5.36E-10 8.20128E-08 8.20128E-08

117.2258

AT2G05520.4 230.943 -6.203926 5.51E-10 8.37362E-08 8.37362E-08

5 0.978246078 AT5G15350.1 177.596 78.8818 1.17083471 6.19863 5.70E-10 8.61319E-08 8.61319E-08

159.0129

AT5G15650.1 66.8221 1.250747036 6.18234 6.32E-10 9.44827E-08 9.44827E-08

5

AT5G49730.1 76.3389 171.75 -6.180081 6.41E-10 9.53323E-08 9.53323E-08

1.169819796

AT4G16720.1 474.944 301.601 0.655116205 6.16751 6.94E-10 1.02133E-07 1.02133E-07

AT5G43850.1 81.42705 178.769 -6.153738 7.57E-10 1.10828E-07 1.10828E-07

1.134516542

AT3G49120.1 99.9597 205.511 -1.03979714 -6.151408 7.68E-10 1.1188E-07 1.1188E-07

114.2347

AT2G05520.5 225.511 -6.145703 7.96E-10 1.15372E-07 1.15372E-07

5 0.981196224

AT3G05590.1 419.8245 258.949 0.697118374 6.130261 8.77E-10 1.26489E-07 1.26489E-07

AT3G62870.1 430.363 267.373 0.686700263 6.123911 9.13E-10 1.30958E-07 1.30958E-07

58.48607

AT5G55450.1 15.1610 2.433299733 6.107217 1.01E-09 1.94833E-07 1.94833E-07

5

AT4G04830.1 34.88045 105.139 -1.59180736 -6.103821 1.04E-09 1.47775E-07 1.47775E-07

AT1G78040.2 336.089 195.052 0.784984533 6.091036 1.12E-09 1.59259E-07 1.59259E-07

AT3G04840.1 447.584 281.936 0.666790759 6.082918 1.18E-09 1.66688E-07 1.66688E-07

AT5G40890.2 34.8311 0 6.551316678 6.08175 1.19E-09 1.67059E-07 1.67059E-07

AT1G13470.1 4.126738 43.148 -6.077901 1.22E-09 1.70258E-07 1.70258E-07

3.386219937

117.2599

AT2G05520.6 228.076 -6.069426 1.28E-09 1.78593E-07 1.78593E-07

5 0.959804296

AT3G02560.1 138.1556 54.844 1.33288834 6.056772 1.39E-09 1.91304E-07 1.91304E-07

AT1G03870.1 100.0647 31.8169 1.65306794 6.055175 1.40E-09 1.92259E-07 1.92259E-07

AT2G36620.1 254.738 135.796 0.907573202 6.010705 1.85E-09 2.50721E-07 2.50721E-07

113.5348

AT1G04680.1 40.2007 1.497842679 6.000396 1.97E-09 2.65874E-07 2.65874E-07

5

ATCG00950.1 561.683 376.251 0.578060722 5.987595 2.13E-09 2.8627E-07 2.8627E-07

AT1G73325.1 59.1657 142.265 -5.982214 2.20E-09 2.94469E-07 2.94469E-07

1.265747821

AT2G14560.2 2.119945 36.3541 -5.966404 2.43E-09 3.22902E-07 3.22902E-07

4.100019339

AT3G51600.1 406.4395 253.408 0.681578547 5.911507 3.39E-09 4.49164E-07 4.49164E-07

AT5G27850.1 360.274 217.794 0.72613033 5.888251 3.90E-09 5.14702E-07 5.14702E-07

AT4G13340.1 114.953 42.3373 1.441042946 5.864949 4.49E-09 5.89667E-07 5.85469E-07

AT1G18540.1 310.8365 180.264 0.786044613 5.864592 4.50E-09 5.88164E-07 5.85469E-07

100.3834

AT5G38980.1 200.259 -5.864577 4.50E-09 5.85469E-07 5.85469E-07

5 0.996345646

AT5G49740.1 70.5982 157.367 -5.86198 4.57E-09 5.91935E-07 5.91935E-07

1.156429733

AT2G39800.1 53.57355 131.5 -5.855327 4.76E-09 6.13275E-07 6.13275E-07

1.295469996

AT1G77940.1 217.966 111.911 0.961751261 5.839812 5.23E-09 6.70105E-07 6.70105E-07

AT3G56340.1 384.657 238.333 0.690593839 5.818779 5.93E-09 7.56622E-07 7.56622E-07

AT1G10370.1 223.5145 116.404 0.941227791 5.806992 6.36E-09 8.04459E-07 8.04459E-07

AT5G15960.1 38.48454 3.34243 3.525309751 -5.805065 6.43E-09 8.10086E-07 8.10086E-07

AT1G15690.1 364.2845 222.731 0.709763243 5.802354 6.54E-09 8.19589E-07 8.19589E-07

AT5G50915.1 46.7127 6.54033 2.8363795 5.794872 6.84E-09 8.53136E-07 8.53136E-07

45.69840

AT1G57720.2 6.19391 2.883221487 5.779446 7.49E-09 9.30982E-07 9.30982E-07

5

AT3G11945.2 31.05615 0 5.275827476 5.766811 8.08E-09 9.99047E-07 9.99047E-07

AT1G76160.1 133.5865 55.0893 1.277930183 5.76326 8.25E-09 1.01579E-06 1.01579E-06

AT1G56280.1 88.9902 181.272 -5.716802 1.09E-08 1.33054E-06 1.33054E-06

1.026437724 AT3G54400.1 91.5691 29.8424 1.617497278 5.702174 1.18E-08 1.44359E-06 1.44359E-06

AT1G70820.1 89.6604 181.739 -5.691833 1.26E-08 1.52712E-06 1.52712E-06

1.019325205

AT4G31700.1 245.3485 134.246 0.869953349 5.689029 1.28E-08 1.54566E-06 1.54566E-06

AT5G39740.1 361.4705 223.044 0.696549573 5.683996 1.32E-08 1.58498E-06 1.57847E-06

AT2G16660.1 179.909 87.2213 1.044514961 5.683961 1.32E-08 1.57847E-06 1.57847E-06

AT5G02960.1 451.5985 294.811 0.615250339 5.682344 1.33E-08 1.58664E-06 1.58664E-06

AT4G05320.6 188.296 312.924 -5.661231 1.50E-08 1.77951E-06 1.77813E-06

0.732809959

AT1G80920.1 31.00335 92.1328 -5.660636 1.51E-08 1.77813E-06 1.77813E-06

1.571290747

AT5G60360.3 64.1305 144.197 -5.659349 1.52E-08 1.78395E-06 1.77969E-06

1.168958589

AT2G34480.1 619.133 433.058 0.515689098 5.659036 1.52E-08 1.77969E-06 1.77969E-06

AT5G60360.2 62.8228 141.295 -5.603589 2.10E-08 2.43421E-06 2.43421E-06

1.169350264

AT1G52000.1 78.9107 164.629 -5.59602 2.19E-08 2.53228E-06 2.53228E-06

1.060925651

AT3G60770.1 317.7625 190.968 0.734617969 5.587015 2.31E-08 2.65609E-06 2.65609E-06

AT1G21600.2 29.50665 0 6.679879862 5.581877 2.38E-08 2.72452E-06 2.72452E-06

AT4G00100.1 323.0825 195.316 0.726092471 5.575697 2.47E-08 2.81148E-06 2.81148E-06

AT4G19200.1 139.7155 247.456 -5.56871 2.57E-08 2.91461E-06 2.91461E-06

0.824679941

AT2G37600.1 102.1552 37.3379 1.45204995 5.560681 2.69E-08 3.03953E-06 3.03953E-06

AT2G36160.1 247.725 138.246 0.841501732 5.555057 2.78E-08 3.12632E-06 3.12632E-06

AT1G23290.1 321.947 195.055 0.722942226 5.544481 2.95E-08 3.2946E-06 3.2946E-06

AT4G13930.1 276.018 160.026 0.786456029 5.52882 3.22E-08 3.58821E-06 3.58821E-06

AT2G16630.1 48.0092 8.29843 2.532400575 5.526857 3.26E-08 3.61413E-06 3.61413E-06

AT5G18380.2 467.176 311.442 0.585002647 5.520335 3.38E-08 3.73599E-06 3.73599E-06

AT3G18490.1 171.271 287.485 -5.517773 3.43E-08 3.77586E-06 3.77586E-06

0.747205791

AT5G01410.1 289.716 435.497 -5.510979 3.57E-08 3.90911E-06 3.90911E-06

0.588023419

AT2G37600.2 2.42516 32.7231 -3.75415759 -5.504283 3.71E-08 4.04471E-06 4.04471E-06

143.1423

AT1G04800.1 64.3029 1.154494862 5.502232 3.75E-08 4.07607E-06 4.07607E-06

5

AT5G02160.1 264.548 403.852 -5.483857 4.16E-08 4.50561E-06 4.50561E-06

0.610297173

AT4G27520.1 477.796 321.122 0.573273269 5.480314 4.25E-08 4.57895E-06 4.57895E-06

AT1G26770.1 38.86326 4.70618 3.045778518 5.474686 4.38E-08 4.69052E-06 4.69052E-06

AT4G01080.1 137.5963 242.321 -5.464126 4.65E-08 4.95939E-06 4.95939E-06

0.816477761

AT3G02020.1 97.5078 187.977 -5.449071 5.06E-08 5.37741E-06 5.37741E-06

0.946966616

AT2G43550.1 257.5345 393.853 -5.43578 5.46E-08 5.77208E-06 5.77208E-06

0.612891552

AT5G47930.1 223.9765 122.846 0.866496487 5.416986 6.06E-08 6.38838E-06 6.38838E-06

AT3G56050.2 4.982875 38.6699 -5.413712 6.17E-08 6.48179E-06 6.48179E-06

2.956160745

AT5G65730.1 262.541 151.868 0.789724828 5.41171 6.24E-08 6.53005E-06 6.53005E-06

AT5G10360.1 310.636 188.908 0.717541236 5.410079 6.30E-08 6.56513E-06 6.56513E-06

AT3G14395.1 50.615 10.0508 2.33225466 5.409257 6.33E-08 6.57073E-06 6.57073E-06

AT2G24200.1 88.28225 174.265 -5.401869 6.60E-08 6.82181E-06 6.82181E-06

0.981087538

AT1G57660.1 341.2315 213.079 0.679362417 5.400654 6.64E-08 6.84272E-06 6.84272E-06 AT4G37450.1 59.97305 14.7015 2.028350983 5.394852 6.86E-08 7.04149E-06 7.04149E-06

13.27769

AT5G09220.1 56.5144 -5.382159 7.36E-08 7.50131E-06 7.50131E-06

5 2.089613799

AT1G73602.1 60.7741 134.404 -5.375043 7.66E-08 7.7752E-06 7.7752E-06

1.145047546

AT2G39800.2 47.2306 113.953 -5.369929 7.88E-08 7.96983E-06 7.96983E-06

1.270645138

AT1G73600.1 60.6468 134.105 -5.36836 7.95E-08 8.01035E-06 8.01035E-06

1.144859599

AT5G63160.1 37.3404 98.0129 -1.39223426 -5.339875 9.30E-08 9.27606E-06 9.27606E-06

AT1G31812.1 300.536 443.38 -5.333703 9.62E-08 9.56283E-06 9.56283E-06

0.561005879

AT2G31880.1 14.16455 57.5917 -2.02357614 -5.322769 1.02E-07 1.01199E-05 1.01199E-05

10.69296

AT2G 18660.1 50.5745 -5.318567 1.05E-07 1.03197E-05 1.03197E-05

05 2.241748813

AT5G20630.1 71.6367 21.4164 1.74198289 5.317716 1.05E-07 1.03314E-05 1.03314E-05

AT5G47330.1 35.02124 3.8129 3.199269567 5.316355 1.06E-07 1.03723E-05 1.03723E-05

AT1G20620.1 119.0748 214.39 -5.308784 1.10E-07 1.07745E-05 1.07745E-05

0.848369489

AT5G28060.1 318.717 197.607 0.689641919 5.289927 1.22E-07 1.19049E-05 1.19049E-05

AT1G03820.1 45.00694 8.06393 2.480592459 5.288678 1.23E-07 1.19447E-05 1.19447E-05

AT3G02480.1 25.8883 0 6.093851992 5.278925 1.30E-07 1.25545E-05 1.25545E-05

AT1G04480.1 265.1155 156.921 0.756582586 5.237496 1.63E-07 1.56715E-05 1.56715E-05

AT1G18080.1 426.722 285.446 0.580078646 5.235222 1.65E-07 1.5811E-05 1.5811E-05

AT4G37990.1 25.3133 0 5.661823693 5.226369 1.73E-07 1.65297E-05 1.65297E-05

AT3G11510.1 346.654 221.064 0.649032305 5.224727 1.74E-07 1.662E-05 1.662E-05

AT5G23740.1 241.908 139.546 0.79371771 5.217596 1.81E-07 1.72136E-05 1.72136E-05

AT3G55500.1 60.69725 16.1713 1.908195493 5.21188 1.87E-07 1.76922E-05 1.76922E-05

AT4G 11600.1 59.63525 129.987 -5.208967 1.90E-07 1.79113E-05 1.79113E-05

1.124130091

AT3G22230.1 294.74 180.5 0.707444029 5.20386 1.95E-07 1.83486E-05 1.83486E-05

AT5G40370.1 168.8965 276.823 -5.198999 2.00E-07 1.87715E-05 1.87715E-05

0.712824383

AT1G29070.1 536.027 376.851 0.508311451 5.195396 2.04E-07 1.90746E-05 1.90746E-05

AT4G36040.1 53.96315 121.009 -5.170692 2.33E-07 2.17044E-05 2.17044E-05

1.165067881

AT1G48630.1 127.47 57.715 1.143139524 5.150937 2.59E-07 2.40397E-05 2.40397E-05

77.32357

AT1G15690.2 154.29 -5.148906 2.62E-07 2.42207E-05 2.42207E-05

45 0.996664323

AT1G70700.2 82.6952 161.649 -5.141054 2.73E-07 2.51716E-05 2.51716E-05

0.966989086

AT1G32640.1 54.92195 121.917 -5.138388 2.77E-07 2.54471E-05 2.54471E-05

1.150444554

AT2G25210.1 167.7465 86.1502 0.961356614 5.121202 3.04E-07 2.77878E-05 2.77878E-05

AT5G36910.1 261.1826 156.14 0.742218613 5.111394 3.20E-07 2.91743E-05 2.91743E-05

AT2G27710.1 225.888 129.394 0.80383691 5.097858 3.44E-07 3.12365E-05 3.12365E-05

AT5G62350.1 225.7375 345.362 -5.094229 3.50E-07 3.17371E-05 3.17371E-05

0.613463251

AT4G13510.1 16.1091 58.594 -5.083922 3.70E-07 3.34008E-05 3.34008E-05

1.862877047

AT2G01020.1 203.1045 112.591 0.851130695 5.079503 3.78E-07 3.40215E-05 3.39629E-05

AT3G41979.1 203.1045 112.591 0.851130695 5.079503 3.78E-07 3.40215E-05 3.39629E-05

AT5G56710.1 180.366 95.8504 0.912071051 5.078911 3.80E-07 3.39629E-05 3.39629E-05

AT2G47610.1 239.894 140.379 0.773069943 5.077347 3.83E-07 3.4134E-05 3.4134E-05

AT1G28400.1 69.54185 21.9971 1.660568109 5.064267 4.10E-07 3.63297E-05 3.63297E-05 AT3G61110.1 219.651 125.507 0.807445237 5.046621 4.50E-07 3.97228E-05 3.97228E-05

AT2G37180.1 96.4126 38.4059 1.327893744 5.044897 4.54E-07 3.99559E-05 3.99559E-05

26.36685

AT2G43620.1 1.60163 4.041112691 5.038608 4.69E-07 4.11607E-05 4.11607E-05

5

AT5G61170.1 267.4925 162.597 0.718197802 5.024322 5.05E-07 4.42062E-05 4.42062E-05

AT2G27530.1 310.8025 197.068 0.657304579 5.003802 5.62E-07 4.90301E-05 4.90301E-05

AT2G37190.1 206.018 116.002 0.828620713 4.998621 5.77E-07 5.02084E-05 5.02084E-05

34.41927

AT2G36590.1 4.74212 2.859612633 4.994948 5.89E-07 5.1014E-05 5.1014E-05

5

AT5G03850.1 274.107 168.361 0.703181188 4.991444 5.99E-07 5.17868E-05 5.17868E-05

AT2G19680.2 24.6494 1.17276 4.393572822 4.983665 6.24E-07 5.3747E-05 5.3747E-05

AT3G09440.1 183.1945 99.3672 0.882534572 4.976688 6.47E-07 5.55482E-05 5.55482E-05

AT5G20700.1 321.366 457.395 -4.969166 6.72E-07 5.7569E-05 5.7569E-05

0.509223293

AT5G35480.1 82.00435 157.24 -4.94976 7.43E-07 6.34213E-05 6.34213E-05

0.939195923

AT1G52870.2 49.98965 111.593 -4.943414 7.68E-07 6.53219E-05 6.53219E-05

1.158545201

AT3G52590.1 419.8655 286.886 0.549449681 4.940893 7.78E-07 6.57699E-05 6.57699E-05

AT5G26340.1 5.49148 35.3731 -4.932854 8.10E-07 6.83288E-05 6.83288E-05

2.687385733

AT2G40750.1 3.83774 31.0582 -3.01664534 -4.899091 9.63E-07 8.04511E-05 8.04511E-05

AT1G27400.1 181.1985 99.1051 0.870539806 4.891737 1.00E-06 8.32669E-05 8.32669E-05

AT3G51730.1 70.7813 140.324 -4.872558 1.10E-06 9.15006E-05 9.15006E-05

0.987321614

AT3G16080.1 193.064 108.381 0.832967311 4.860871 1.17E-06 9.67832E-05 9.67832E-05

AT2G36530.1 466.2445 327.528 0.509468461 4.855503 1.20E-06 9.91474E-05 9.91474E-05

AT3G50480.1 9.601025 43.5373 -2.1809916 -4.852257 1.22E-06 0.000100486 0.000100486

AT2G21140.1 37.23775 6.52483 2.512753685 4.846088 1.26E-06 0.000103354 0.000103354

AT5G16130.1 270.425 168.205 0.685007939 4.843342 1.28E-06 0.000104179 0.000104179

AT4G12730.1 196.582 111.348 0.820055582 4.838973 1.31E-06 0.000106184 0.000106184

AT5G42300.1 155.1895 250.812 -4.826642 1.39E-06 0.000112642 0.000112642

0.692575426

AT5G63800.1 41.3443 96.8591 -4.821824 1.42E-06 0.000115063 0.000115063

1.228199154

AT4G39730.1 215.876 325.826 -4.808626 1.52E-06 0.000122568 0.000122568

0.593898871

AT1G08450.1 27.68435 75.2494 -4.804975 1.55E-06 0.000124465 0.000124465

1.442609429

AT2G26440.1 7.865295 39.4411 -4.802188 1.57E-06 0.000125847 0.000125847

2.326127006

AT4G34670.1 253.273 155.599 0.702860499 4.79601 1.62E-06 0.000129416 0.000129416

AT1G01620.2 132.2735 65.2952 1.018475213 4.772631 1.82E-06 0.000144973 0.000144973

AT2G28190.1 76.8872 28.5546 1.429020267 4.766176 1.88E-06 0.000149266 0.000149266

AT4G08950.1 27.71085 2.94495 3.23413592 4.751912 2.02E-06 0.000159284 0.000159284

AT5G36700.2 60.8255 19.1552 1.666940185 4.749275 2.04E-06 0.000160918 0.000160918

AT1G52300.1 373.3325 253.102 0.560742199 4.7469 2.07E-06 0.000162358 0.000162358

AT3G14420.6 292.068 187.44 0.639875441 4.734595 2.19E-06 0.000172039 0.000172039

AT1G73480.1 29.46285 77.121 -4.726393 2.29E-06 0.00017813 0.00017813

1.388226767

1.509807

AT3G49580.1 23.3208 -4.725132 2.30E-06 0.000178738 0.000178738

5 3.949180757

AT1G52870.1 44.2545 99.8168 -4.722812 2.33E-06 0.000180286 0.000180286

1.173458491

AT1G70410.1 21.93812 0 4.473385485 4.721415 2.34E-06 0.000181025 0.000181025 25

AT3G11120.1 200.42 116.073 0.78799406 4.719126 2.37E-06 0.000182566 0.000182566

AT5G54940.1 64.36515 128.67 -4.712243 2.45E-06 0.000188322 0.000188322

0.999324053

AT2G17040.1 5.346735 33.0479 -2.62782852 -4.711003 2.47E-06 0.00018895 0.00018895

AT3G02230.1 96.5691 41.6563 1.21302693 4.702118 2.57E-06 0.000196821 0.000196821

AT3G23390.1 220.109 131.443 0.743780823 4.700933 2.59E-06 0.000197424 0.000197424

AT1G20620.5 112.8033 193.66 -4.69683 2.64E-06 0.000200879 0.000200879

0.779716726

AT1G35720.1 87.4434 159.684 -4.674216 2.95E-06 0.000223113 0.000223113

0.868798362

AT1G65800.1 11.9294 46.3172 -4.670619 3.00E-06 0.000226441 0.000226441

1.957026558

AT3G18080.1 97.6601 173.235 -4.669744 3.02E-06 0.000226793 0.000226793

0.826889278

AT2G15620.1 333.763 222.69 0.583787256 4.656843 3.21E-06 0.000240174 0.000240174

AT5G54940.2 83.38085 153.883 -4.65595 3.22E-06 0.00024057 0.00024057

0.884045876

102.1920

AT1G04430.1 45.902 1.154654047 4.649484 3.33E-06 0.000246913 0.000246913

5

AT3G56020.1 147.575 77.7368 0.924778716 4.648385 3.35E-06 0.000247572 0.000247572

AT5G05270.1 33.90255 5.86501 2.531188317 4.643156 3.43E-06 0.000252579 0.000252579

AT4G36130.1 165.621 91.0596 0.863002585 4.641883 3.45E-06 0.000253471 0.000253471

AT3G47480.1 7.940425 38.0335 -4.634931 3.57E-06 0.00026145 0.00026145

2.259982575

20.07786

AT2G03680.2 0 5.211350506 4.631522 3.63E-06 0.000265095 0.000265095

9

34.59604

AT3G57260.1 3.7950 2.363558825 4.620691 3.82E-06 0.000401163 0.000401163

5

19.88279

AT2G23130.2 0 5.180853404 4.606231 4.10E-06 0.00029867 0.00029867

5

AT3G26280.1 8.263135 38.3708 -4.599146 4.24E-06 0.000308202 0.000308202

2.215247701

AT5G03240.2 76.3518 143.056 -4.581459 4.62E-06 0.000333714 0.000333714

0.905845934

AT3G14067.1 64.18025 126.092 -4.568636 4.91E-06 0.000353873 0.000353873

0.974275431

AT3G59540.1 266.0445 170.021 0.645954628 4.557391 5.18E-06 0.000371427 0.000371427

AT5G19240.1 59.28965 118.637 -4.529883 5.90E-06 0.000422142 0.000422142

1.000701836

116.2725

AT3G18130.1 56.8121 1.033240404 4.52844 5.94E-06 0.000423943 0.000423943

5

AT2G32060.1 7.911885 36.9795 -4.52668 5.99E-06 0.000426395 0.000426395

2.224632357

AT1G09690.1 409.8555 288.564 0.506224031 4.525364 6.03E-06 0.000427963 0.000427963

AT4G26230.1 167.6455 94.1817 0.831895089 4.524495 6.05E-06 0.000428631 0.000428631

AT5G19590.1 63.8111 124.859 -4.523744 6.08E-06 0.000429065 0.000429065

0.968420507

AT5G45950.1 88.21415 37.8044 1.222455933 4.521877 6.13E-06 0.000431771 0.000431771

AT3G05220.2 0 19.2534 -4.499957 6.80E-06 0.000477573 0.000477573

4.791653411

AT4G34920.1 51.17365 106.596 -4.495132 6.95E-06 0.0004873 0.0004873

1.058680259

AT5G19510.1 306.2095 203.634 0.58854058 4.493571 7.00E-06 0.000489656 0.000489656

AT3G16460.1 3.602485 26.9743 -4.482677 7.37E-06 0.000514047 0.000513208

2.904521193

AT2G32680.1 3.882205 27.6563 -4.482491 7.38E-06 0.000513208 0.000513208

2.832659952

AT1G09590.1 248.8525 157.873 0.656526423 4.472535 7.73E-06 0.000535028 0.000535028

AT5G19250.1 108.457 183.601 -4.47129 7.77E-06 0.000536817 0.000536817

0.759450747 AT1G49500.1 71.62675 134.327 -4.444913 8.79E-06 0.000604095 0.000604095

0.907178931

AT4G31700.2 162.8475 91.7583 0.827611004 4.439366 9.02E-06 0.000618348 0.000618348

AT5G02450.1 255.9535 164.212 0.640322177 4.434938 9.21E-06 0.0006281 0.0006281

AT3G04720.1 5.205575 30.3464 -4.434169 9.24E-06 0.000628806 0.000628806

2.543395944

AT1G33120.1 322.121 218.076 0.562771712 4.423858 9.70E-06 0.000657984 0.000657984

AT5G56670.1 269.8235 175.667 0.619172805 4.417299 9.99E-06 0.000676614 0.000676123

AT2G15960.1 41.6755 91.6976 -4.416931 1.00E-05 0.000676123 0.000676123

1.137684467

AT5G57040.1 166.058 254.982 -4.409911 1.03E-05 0.000696723 0.000696723

0.618708178

AT1G02920.1 4.983335 29.5522 -4.39923 1.09E-05 0.000730141 0.000730141

2.568082069

AT2G16600.1 75.03555 138.133 -4.396027 1.10E-05 0.000739208 0.000739208

0.880411845

AT5G14200.1 132.0217 211.89 -4.380381 1.18E-05 0.000792423 0.000792423

0.682540422

AT1G70580.3 8.34782 36.5202 -4.37807 1.20E-05 0.000798949 0.000797549

2.129223268

AT4G32340.1 37.9247 85.5793 -1.17412411 -4.374971 1.21E-05 0.000804596 0.00080286

AT3G53870.1 368.6355 257.67 0.516670434 4.373824 1.22E-05 0.000805006 0.000805006

AT4G12420.1 121.1695 62.0311 0.965962985 4.369996 1.24E-05 0.000817312 0.000817312

AT5G07090.1 191.897 115.084 0.737644886 4.35729 1.32E-05 0.000862135 0.000860448

AT1G22930.1 9.68981 38.9233 -4.351342 1.35E-05 0.000881712 0.000881712

2.006093748

19.33259

AT4G28140.1 1.08286 4.15811659 4.350527 1.36E-05 0.000882928 0.000882928

4

AT2G39460.1 135.815 72.9033 0.897586801 4.347418 1.38E-05 0.000893447 0.000893447

AT1G19660.1 17.8959 53.6328 -4.342999 1.41E-05 0.000909495 0.000909495

1.583486474

AT5G59850.1 206.154 126.737 0.701884683 4.321504 1.55E-05 0.001000471 0.001000471

AT1G74670.1 146.062 80.9283 0.851864695 4.310643 1.63E-05 0.001046041 0.001046041

AT3G45030.1 171.8965 100.449 0.775076969 4.307594 1.65E-05 0.001058118 0.001058072

19.37297

AT4G30290.1 1.20805 4.003293221 4.306587 1.66E-05 0.001058072 0.001058072

2

AT5G24120.1 33.2371 77.5837 -4.301039 1.70E-05 0.001082432 0.001082432

1.222959071

AT1G70810.1 26.8441 67.6598 -4.29452 1.75E-05 0.001112184 0.001112184

1.333693876

AT5G47240.1 19.6022 55.9484 -4.291632 1.77E-05 0.001124186 0.00112216

1.513081294

AT4G05320.5 138.2835 217.9 -4.29137 1.78E-05 0.001122957 0.00112216

0.656037172

AT3G55750.1 174.0625 102.343 0.766192993 4.291023 1.78E-05 0.00112216 0.00112216

35.66851

AT5G65390.1 8.17107 2.126054236 4.288471 1.80E-05 0.00113256 0.00113256

5

17.88963

AT2G02990.1 0 7.194432604 4.282171 1.85E-05 0.00115987 0.00115987

53

AT2G45470.1 309.285 210.51 0.555048098 4.280122 1.87E-05 0.00116535 0.00116535

AT5G44568.1 27.47175 68.4234 -4.276185 1.90E-05 0.001183484 0.001183484

1.316540979

AT5G02870.1 206.932 128.137 0.691469642 4.272328 1.93E-05 0.00120146 0.00120146

AT3G09500.1 324.7455 223.491 0.539092799 4.269597 1.96E-05 0.001213555 0.001213555

AT4G05070.1 30.8337 73.4221 -4.260488 2.04E-05 0.00126128 0.00126128

1.251706357

AT1G22780.1 171.975 101.211 0.764832759 4.258558 2.06E-05 0.001269398 0.001269398

AT2G01250.1 236.565 151.875 0.639352233 4.257789 2.06E-05 0.001270952 0.001270952

AT2G24850.1 15.05075 47.754 - -4.248892 2.15E-05 0.001319572 0.001319572 1.665786202

4.637550

AT1G56120.1 27.5249 -4.246765 2.17E-05 0.001329219 0.001329219

5 2.569302425

AT3G45970.1 31.7402 6.45287 2.298298353 4.245837 2.18E-05 0.001331802 0.001331802

AT5G23020.1 50.26739 101.623 -4.243162 2.20E-05 0.00134483 0.00134483

1.015532271

127.0074

AT4G05320.1 202.301 -4.220273 2.44E-05 0.001485662 0.001485662

5 0.671590326

AT3G02470.4 223.374 142.075 0.652808555 4.215753 2.49E-05 0.001512432 0.001512432

26.51602

AT2G05540.1 4.22082 2.651269253 4.213167 2.52E-05 0.001526526 0.001525341

4

AT1G20693.1 32.93125 76.0091 -4.212851 2.52E-05 0.001525341 0.001525341

1.206714877

100.7092

AT5G09510.1 49.396 1.027730074 4.195804 2.72E-05 0.001637611 0.001637611

5

AT4G38550.1 31.44855 73.4867 -4.19002 2.79E-05 0.00167629 0.00167629

1.224489667

17.59498

AT4G39320.1 0 4.276634861 4.18217 2.89E-05 0.001727779 0.001727779

5

AT4G04330.1 18.38745 52.7439 -4.181412 2.90E-05 0.001729832 0.001729832

1.520282833

19.01078

AT5G22500.1 1.41182 3.751189961 4.175397 2.97E-05 0.001772381 0.001772381

4

AT5G19190.1 86.40955 39.7393 1.120624308 4.172982 3.01E-05 0.001787451 0.001787451

AT3G49010.1 22.44765 59.1488 -1.39778448 -4.160602 3.17E-05 0.001883144 0.00188269

AT2G23130.1 82.84035 37.4389 1.145795602 4.16017 3.18E-05 0.00188269 0.00188269

AT1G69620.1 259.096 171.972 0.591313054 4.151115 3.31E-05 0.001954596 0.00195404

AT1G13360.1 50.3716 100.439 -4.150695 3.31E-05 0.00195404 0.00195404

0.995637107

AT5G47700.1 158.847 92.8125 0.775246818 4.141634 3.45E-05 0.002028584 0.00202649

AT1G20620.4 157.0028 237.858 -4.141386 3.45E-05 0.00202649 0.00202649

0.599310261

AT1G04820.1 157.274 91.6391 0.779244994 4.139691 3.48E-05 0.00203722 0.00203722

AT1G56045.1 269.169 180.44 0.576993074 4.137366 3.51E-05 0.002053644 0.002053644

AT3G09200.2 211.6115 134.171 0.657345155 4.128873 3.65E-05 0.00212652 0.00212652

AT2G47130.1 2.116742 20.4663 -4.126664 3.68E-05 0.002142546 0.002142546

3.273332967

AT3G10020.2 8.846985 35.2453 -4.12466 3.71E-05 0.002152277 0.002152277

1.994173103

AT3G06700.1 219.815 140.855 0.642079061 4.120045 3.79E-05 0.002191285 0.002191285

AT2G04780.1 63.43555 25.0704 1.339306555 4.119342 3.80E-05 0.002193418 0.002193418

AT2G37710.1 20.81545 55.9775 -4.111163 3.94E-05 0.002258536 0.002257239

1.427192309

AT4G33865.1 289.5415 197.804 0.549698544 4.104491 4.05E-05 0.002315163 0.002315163

AT3G53460.1 277.0895 187.699 0.561931079 4.097288 4.18E-05 0.002383481 0.002380101

11.26915

AT4G27740.1 39.5408 -4.097142 4.18E-05 0.002380101 0.002380101

5 1.810962719

AT5G01750.2 180.5685 265.477 -4.094373 4.23E-05 0.002403813 0.002403813

0.556040638

39.74885

AT5G36700.3 84.485 -4.090277 4.31E-05 0.002441675 0.002441675

5 1.087781916

AT2G44290.1 31.4387 71.9526 -4.066296 4.78E-05 0.002695934 0.002695934

1.194505257

AT3G22600.1 38.85114 10.8788 1.836437505 4.062442 4.86E-05 0.0027353 0.0027353

AT5G64310.1 28.34837 5.60128 2.339437331 4.055639 5.00E-05 0.002810466 0.002810466

AT1G41880.1 216.1145 139.019 0.636513799 4.053238 5.05E-05 0.002833753 0.002833753

AT5G02870.2 180.921 111.211 0.702060382 4.049256 5.14E-05 0.002876595 0.002876595

AT3G51450.1 26.1406 63.6222 -4.043047 5.28E-05 0.002936146 0.002936146

1.283238004 AT3G56090.1 114.0505 60.6298 0.911573803 4.036714 5.42E-05 0.003010493 0.003010493

AT3G15840.2 72.9656 129.284 -4.027281 5.64E-05 0.003127572 0.003127572

0.825255377

AT1G78040.1 164.6155 99.1714 0.731104157 4.003977 6.23E-05 0.00343192 0.00343192

AT1G19660.2 14.32745 44.0369 -4.000083 6.33E-05 0.003481996 0.003481996

1.619931052

AT3G16670.1 82.72135 38.8801 1.089227801 3.989334 6.63E-05 0.003636406 0.003636406

AT3G15840.1 73.22795 128.849 -3.978998 6.92E-05 0.003783239 0.003783239

0.815215027

AT4G23260.1 10.79297 37.5543 -3.974701 7.05E-05 0.003837112 0.00383111

1.798886188

AT1G14345.1 165.107 99.9701 0.723832717 3.974608 7.05E-05 0.00383111 0.00383111

AT4G14320.1 293.7255 204.063 0.525453898 3.9655 7.32E-05 0.003972635 0.003972635

AT2G17360.1 215.522 140.058 0.621810753 3.963007 7.40E-05 0.00400656 0.00400656

AT4G31985.1 246.511 165.211 0.577342278 3.961551 7.45E-05 0.004023251 0.004023251

AT2G29550.1 64.8752 27.1722 1.255535703 3.960691 7.47E-05 0.00402995 0.00402995

AT1G43170.1 159.6775 95.9774 0.734394402 3.959153 7.52E-05 0.004048135 0.004048135

AT3G28940.1 60.286 111.25 -3.958214 7.55E-05 0.004056227 0.004056227

0.883910422

AT1G24145.1 3.00834 21.4763 -3.952189 7.74E-05 0.004151744 0.004144992

2.835705929

AT3G17020.1 54.64205 103.466 -3.95066 7.79E-05 0.004162313 0.004152025

0.921073246

AT1G16720.1 107.7315 172.786 -3.950505 7.80E-05 0.004157042 0.004152025

0.681546181

AT1G66330.1 13.3654 41.8977 -3.950336 7.80E-05 0.004152025 0.004152025

1.648368033

12.58668

AT4G37150.1 40.4872 -3.944946 7.98E-05 0.004238423 0.004238423

5 1.685567508

AT3G62820.1 70.5978 31.076 1.18382241 3.942799 8.05E-05 0.004268415 0.004268415

AT1G55490.2 294.1845 205.001 0.521090289 3.938081 8.21E-05 0.004344931 0.004344931

AT4G39200.1 203.697 131.076 0.63602118 3.932296 8.41E-05 0.004433993 0.004433993

AT4G34950.1 260.2055 177.043 0.555551654 3.929042 8.53E-05 0.004485929 0.004481208

AT2G24200.2 89.89505 44.4291 1.01673676 3.928841 8.54E-05 0.004481208 0.004481208

AT5G45380.1 4.34361 24.3753 -3.925682 8.65E-05 0.00451487 0.00451487

2.488453496

AT4G15530.3 5.433125 26.7065 -3.922377 8.77E-05 0.004568696 0.004558671

2.297336772

AT2G29670.1 44.8778 89.5463 -3.922353 8.77E-05 0.004560614 0.004558671

0.996631868

AT1G78680.1 76.57135 132.282 -3.920018 8.85E-05 0.004587876 0.004587876

0.788740165

AT3G28900.1 118.236 65.0822 0.861334443 3.915429 9.02E-05 0.004667342 0.004667342

AT1G71030.1 59.28655 109.213 -3.912355 9.14E-05 0.004709659 0.004709659

0.881367844

AT3G11940.2 194.898 124.425 0.647442899 3.90867 9.28E-05 0.004773219 0.004773219

AT1G29670.1 168.6545 103.702 0.701627094 3.907406 9.33E-05 0.004789412 0.004789412

AT5G56710.2 99.81805 51.7525 0.947672164 3.902881 9.51E-05 0.004861926 0.004861926

AT4G20110.1 1.37375 16.8839 -3.902234 9.53E-05 0.004866007 0.004866007

3.619456803

AT1G07890.8 93.20835 47.06 0.985957883 3.899216 9.65E-05 0.00491802 0.00491802

14.86674

AT3G22620.1 0 7.244620388 3.896877 9.74E-05 0.004956666 0.0049486

55

AT1G24020.1 45.96455 15.8094 1.539739001 3.896829 9.75E-05 0.0049486 0.0049486

AT3G19010.1 16.1142 46.0219 -3.894035 9.86E-05 0.004996857 0.004996857

1.513987979

AT1G65790.1 4.724616 24.9451 -3.890243 0.0001001 0.005066365 0.00505291

2.400487471 AT4G15440.1 60.80035 110.917 -3.890008 0.0001002 0.00505291 0.00505291

0.867328967

AT1G14320.1 291.3175 203.634 0.516613907 3.887635 0.0001012 0.005093317 0.005093317

AT1G06460.1 108.4214 172.413 -3.884025 0.0001027 0.005160254 0.005151361

0.669219017

AT3G07110.1 117.3875 64.8146 0.856888058 3.884006 0.0001027 0.005151361 0.005151361

AT3G15536.1 0 14.7836 -3.882304 0.0001035 0.005178222 0.00516918

4.432086848

AT2G41410.1 70.83545 124.144 -3.882292 0.0001035 0.00516918 0.00516918

0.809471085

AT2G21580.1 131.8296 75.6834 0.800625531 3.880737 0.0001041 0.005193016 0.005193016

AT3G06680.2 64.5535 27.5728 1.227249539 3.880219 0.0001044 0.005194778 0.005194778

AT5G60900.1 4.537915 24.3877 -3.870171 0.0001088 0.005403958 0.005397258

2.426052213

AT3G08030.1 92.44265 46.8061 0.981862056 3.869641 0.000109 0.005406068 0.005397258

AT1G27970.1 72.0882 32.7808 1.136912063 3.856413 0.0001151 0.005686737 0.005686737

AT5G44580.1 67.87095 119.802 -3.854986 0.0001157 0.005699743 0.005699743

0.819785881

AT2G15020.1 34.35115 73.6516 -3.854426 0.000116 0.005702722 0.005702722

1.100358468

AT1G11910.1 192.7875 274.367 -3.847398 0.0001194 0.005858421 0.005853555

0.509095456

AT2G27720.1 151.4485 91.1964 0.731778509 3.84362 0.0001212 0.005928475 0.005928475

AT2G47110.1 230.8265 154.599 0.578277874 3.839104 0.0001235 0.006027974 0.006013843

AT4G24350.1 21.9515 54.8501 -3.838912 0.0001236 0.006022128 0.006013843

1.321174725

AT3G15840.4 62.51505 112.441 -3.83882 0.0001236 0.006013843 0.006013843

0.846892736

AT3G16780.1 95.5603 49.365 0.952922856 3.836584 0.0001248 0.006058252 0.006058252

AT1G08450.2 17.5863 47.8418 -3.833629 0.0001263 0.006120842 0.006120842

1.443819686

AT3G55280.3 85.22735 42.0601 1.018864195 3.832132 0.000127 0.006147516 0.006147516

27.81259

AT1G72910.1 63.7041 -3.828991 0.0001287 0.006215677 0.006215677

5 1.195647869

AT3G54600.1 135.5905 204.984 -3.827202 0.0001296 0.00625017 0.00625017

0.596255204

AT5G13490.1 30.75745 7.70148 1.997728278 3.825102 0.0001307 0.006292779 0.006292779

AT1G17880.1 201.8825 131.42 0.61933101 3.821667 0.0001326 0.006370067 0.006370067

AT2G34510.1 68.3158 30.5636 1.160404798 3.816732 0.0001352 0.006487584 0.006487584

AT5G23900.1 156.6775 95.7283 0.710780619 3.809771 0.0001391 0.006661507 0.006661507

AT4G22010.1 44.50175 15.5337 1.518460561 3.795006 0.0001476 0.007058562 0.007058562

AT3G53460.2 244.442 166.607 0.553043173 3.792329 0.0001492 0.007122864 0.007122864

AT5G09440.1 24.29697 4.6934 2.372071085 3.785757 0.0001532 0.00730126 0.00730126

AT2G05220.1 136.243 80.2575 0.763473986 3.784439 0.0001541 0.007327534 0.007327534

AT3G58610.2 182.814 116.923 0.644817806 3.771702 0.0001621 0.007685654 0.007685654

AT2G30520.2 26.7304 5.91234 2.176680285 3.768011 0.0001646 0.007773681 0.007773681

AT5G24210.1 36.603 75.7976 -3.765861 0.000166 0.007827607 0.007827607

1.050190271

AT1G72370.1 264.0975 183.591 0.524575308 3.754313 0.0001738 0.008169706 0.008169706

AT5G19770.1 175.744 111.564 0.655603869 3.753695 0.0001742 0.008176101 0.008176101

AT1G26880.1 125.022 72.1306 0.793498657 3.749827 0.000177 0.008289243 0.008289243

1.043211

AT1G18150.2 14.9293 -3.746214 0.0001795 0.008395345 0.008395345

5 3.839042939

AT5G20720.1 275.706 193.572 0.510260381 3.738834 0.0001849 0.008631165 0.008631165

AT4G 18730.1 203.8 134.336 0.601308075 3.738151 0.0001854 0.00864012 0.00864012 AT3G53020.1 265.962 185.687 0.518347316 3.726104 0.0001945 0.009033228 0.009033228

AT2G43970.1 120.6245 184.502 -3.721265 0.0001982 0.009192776 0.009192776

0.613113493

2.040674

AT5G55460.1 17.4505 -3.717178 0.0002015 0.009311694 0.009311694

5 3.096150387

AT5G07090.2 160.0175 99.6567 0.683190984 3.716654 0.0002019 0.009315525 0.009315525

AT1G23390.1 71.23 122.022 -3.715322 0.0002029 0.009349266 0.009349266

0.776584387

AT4G15000.1 160.8545 100.342 0.680830698 3.714848 0.0002033 0.009351313 0.009351313

AT4G15000.2 170.427 107.957 0.658697122 3.712144 0.0002055 0.009436202 0.009436202

AT2G40590.1 134.0685 79.5196 0.753587904 3.711311 0.0002062 0.009451727 0.009451727

AT3G28220.1 258.9823 180.226 0.523046347 3.707738 0.0002091 0.0095703 0.0095703

23.95319

AT3G03341.1 4.83662 2.308147094 3.697959 0.0002173 0.009913929 0.009913929

65

AT4G24350.2 17.767 46.727 -3.692556 0.000222 0.010110415 0.010110415

1.395056316

AT2G31610.1 206.279 137.147 0.588873892 3.68989 0.0002244 0.010200286 0.010200286

15.17815

AT3G14620.1 42.4533 -1.48388027 -3.685225 0.0002285 0.010371991 0.010371991

5

AT5G42050.1 40.45025 80.1281 -3.678349 0.0002347 0.010620932 0.010620932

0.986159649

AT1G01620.1 130.1535 76.9638 0.757962171 3.675394 0.0002375 0.010727196 0.010727196

AT5G02290.1 5.313335 24.5548 -3.672054 0.0002406 0.010850812 0.010850812

2.208315491

AT1G70090.1 27.23075 6.54858 2.055982708 3.667316 0.0002451 0.011035888 0.011035888

AT4G10500.1 1.936441 16.8354 -3.663961 0.0002483 0.01116345 0.011160861

3.120018548

AT5G54585.1 7.822255 29.3742 -3.658799 0.0002534 0.011354052 0.011354052

1.908893088

AT4G34630.1 26.82615 60.2975 -3.658279 0.0002539 0.01135879 0.01135879

1.168458169

AT5G60670.1 113.3605 64.4409 0.814869478 3.654286 0.0002579 0.011518496 0.011511906

AT5G62300.1 127.003 74.8122 0.763517114 3.654022 0.0002582 0.011511906 0.011511906

114.1013

AT1G50010.1 65.0921 0.809761497 3.646213 0.0002661 0.011848252 0.011838167

5

AT2G38310.1 41.60895 14.6564 1.5053631 3.646021 0.0002663 0.011838167 0.011838167

AT4G10480.1 157.401 98.7428 0.672697246 3.635417 0.0002775 0.01229667 0.01229667

2.573757

AT3G61280.1 18.2311 -3.634037 0.000279 0.012343022 0.012343022

5 2.824453577

AT1G51402.1 44.57105 85.2262 -0.93519006 -3.631096 0.0002822 0.012464724 0.012464724

AT1G43170.3 147.9435 91.4055 0.69469343 3.627348 0.0002863 0.012626944 0.012618962

AT1G43170.2 148.202 91.6117 0.693961151 3.627103 0.0002866 0.012618962 0.012618962

AT5G47560.1 65.289 112.868 -3.624777 0.0002892 0.012712952 0.012711581

0.789724666

AT2G37040.1 83.36525 42.6804 0.965872438 3.624397 0.0002896 0.012711581 0.012711581

AT4G29110.1 4.750885 22.9965 -3.618608 0.0002962 0.012938068 0.012938068

2.275146114

AT4G35750.1 20.58515 50.392 -3.616212 0.0002989 0.013037879 0.013037879

1.291590755

AT5G40890.1 39.2044 77.5505 -3.612556 0.0003032 0.013181836 0.013181836

0.984120503

AT2G36170.1 146.575 90.5977 0.694092727 3.607745 0.0003089 0.013407626 0.013407626

AT4G25630.1 46.8327 18.0976 1.371717827 3.605322 0.0003118 0.013512294 0.013512294

AT1G21310.1 5.43912 24.3046 -2.15978423 -3.603494 0.000314 0.013586549 0.013586549

AT1G44350.1 30.4341 64.9462 -3.601501 0.0003164 0.013669927 0.013669927

1.093556413

AT3G11010.1 2.50068 17.8366 -3.600943 0.0003171 0.013678049 0.013678049

2.834448276 175.7573

AT5G24780.1 113.916 0.625614629 3.59854 0.00032 0.013783649 0.013783649

5

AT4G01390.1 0 12.6783 -3.592347 0.0003277 0.014093696 0.014093696

4.416609511

AT5G02790.1 138.1735 203.342 -3.591167 0.0003292 0.014135824 0.014135824

0.557427282

AT3G04920.1 200.002 133.683 0.581198412 3.590031 0.0003306 0.014175707 0.014175707

AT2G38750.1 65.0826 112.013 -3.586032 0.0003357 0.014372585 0.014372585

0.783322386

AT1G74050.1 189.3245 125.116 0.597594824 3.582664 0.0003401 0.014536879 0.014536879

AT3G26180.2 12.13213 36.374 -3.577533 0.0003469 0.014779741 0.014779741

1.584074724

AT5G03240.1 69.64555 117.803 -0.7582732 -3.576702 0.000348 0.014804112 0.014804112

AT3G02080.1 232.522 160.7 0.532997294 3.574943 0.0003503 0.01488122 0.01488122

AT3G26450.1 117.8318 178.217 -0.59690602 -3.571977 0.0003543 0.015005052 0.015005052

AT2G26560.1 17.45965 45.0435 -3.57085 0.0003558 0.01504682 0.01504682

1.367294294

AT5G21020.2 43.5136 82.9645 -3.56936 0.0003579 0.015109735 0.015089939

0.931027768

AT3G04290.1 21.31906 4.04385 2.398342439 3.569306 0.0003579 0.015089939 0.015089939

AT3G09630.1 141.656 87.2829 0.698620768 3.567305 0.0003607 0.015159624 0.015159624

11.79340

AT1G43160.1 0 5.978682576 3.566008 0.0003625 0.015211864 0.015211864

55

14.96870

AT1G54575.1 40.912 -3.55744 0.0003745 0.015692885 0.015692885

5 1.450574652

AT1G23205.1 43.1845 16.1266 1.421071281 3.556829 0.0003754 0.015705774 0.015705774

AT4G16520.1 41.2548 79.6748 -3.55544 0.0003773 0.015765267 0.015765267

0.949561506

AT1G66580.1 204.8385 138.274 0.566956991 3.551604 0.0003829 0.015972891 0.015972891

AT4G26690.1 75.55445 37.7975 0.999225915 3.550041 0.0003852 0.01602004 0.01602004

AT2G29170.1 12.38437 36.5425 -3.548103 0.000388 0.016114282 0.016114282

1.561054857

AT3G14600.1 135.832 83.1466 0.708094221 3.535333 0.0004073 0.016838131 0.016815064

AT5G35530.1 142.7585 88.5753 0.688600296 3.535303 0.0004073 0.016815064 0.016815064

AT1G09070.1 60.24825 104.886 -3.532294 0.000412 0.016982367 0.016982367

0.799830881

AT1G08360.1 234.445 163.1 0.523492729 3.530467 0.0004148 0.017049553 0.017049553

AT5G24155.1 41.8267 80.1262 -0.93784988 -3.529066 0.000417 0.017114821 0.017114821

AT3G51240.1 49.32555 20.1973 1.288172693 3.523938 0.0004252 0.017398156 0.017387727

AT4G 14020.1 19.12295 47.1917 -3.523707 0.0004256 0.017387727 0.017387727

1.303228045

AT2G29630.1 121.496 181.724 -0.58084015 -3.521019 0.0004299 0.017539186 0.017539186

AT1G07770.1 191.237 127.746 0.582083561 3.515355 0.0004392 0.01786524 0.01786524

AT1G72370.2 215.5775 147.76 0.544950839 3.513348 0.0004425 0.017974446 0.017974446

AT5G63780.1 36.0298 71.8538 -3.511348 0.0004458 0.018083804 0.018083804

0.995873812

AT2G23840.1 25.43675 56.5974 -3.509064 0.0004497 0.018213264 0.018213264

1.153821427

AT2G04570.1 36.25685 12.2237 1.568572547 3.508528 0.0004506 0.018223486 0.018223486

101.8616

AT2G43970.2 157.316 -3.504732 0.0004571 0.01843174 0.01843174

5 0.627054417

AT5G51550.1 62.29865 29.0113 1.102585956 3.496688 0.0004711 0.018969031 0.018969031

AT5G53350.1 39.0402 75.8594 -3.495811 0.0004726 0.019003961 0.019003961

0.958367517

148.8668

AT2G25450.1 214.164 -3.492073 0.0004793 0.019244097 0.019244097

2 0.524693754

AT2G20450.1 132.7545 81.3493 0.706558928 3.488308 0.0004861 0.019489069 0.019489069 AT3G07110.2 94.4911 52.2733 0.854104211 3.4749 0.000511 0.020430773 0.020430773

AT2G37270.1 125.5275 75.9206 0.725440159 3.472378 0.0005159 0.020594096 0.020594096

AT4G15800.1 86.8944 46.7384 0.894654857 3.467612 0.0005251 0.020902786 0.020902786

AT3G52400.1 3.903295 20.0947 -3.460568 0.000539 0.02142676 0.02142676

2.364050632

AT4G01610.1 13.42655 37.4812 -3.457873 0.0005445 0.021580571 0.021573386

1.481078496

AT3G44010.1 191.9815 129.284 0.570423554 3.457579 0.0005451 0.021573386 0.021573386

AT4G12600.1 101.0628 57.3862 0.816476306 3.456277 0.0005477 0.021647067 0.021647067

AT5G43150.1 17.43441 43.8824 -3.454588 0.0005511 0.021752205 0.021752205

1.331704889

25.43309

AT3G01290.1 55.9695 -3.451333 0.0005578 0.021985081 0.021985081

5 1.137933823

AT5G19780.1 120.443 72.2518 0.737245113 3.450253 0.0005601 0.022041989 0.022027671

AT2G44120.1 124.886 75.6991 0.722263704 3.450047 0.0005605 0.022027671 0.022027671

127.1249

AT5G16010.1 77.452 0.714872808 3.449214 0.0005622 0.022064586 0.022064586

5

AT4G15210.1 52.92815 23.0609 1.19858641 3.447939 0.0005649 0.022137829 0.022137829

AT2G37970.1 33.0581 66.9049 -3.447192 0.0005664 0.022167831 0.022167831

1.017108067

AT1G72430.1 83.0429 44.1414 0.911724439 3.444974 0.0005711 0.022319164 0.022319164

AT3G11940.1 146.9755 93.1789 0.657500482 3.441624 0.0005782 0.022502541 0.022502541

AT2G03310.1 20.23 48.0341 -3.437147 0.0005879 0.022845928 0.022845928

1.247562637

AT3G07310.1 34.43935 68.7102 -3.435347 0.0005918 0.022966093 0.022959549

0.996466368

AT5G08640.1 34.404 11.5135 1.579249844 3.434731 0.0005931 0.022986345 0.022959549

AT3G61280.2 2.303189 16.2955 -3.43467 0.0005933 0.022959549 0.022959549

2.822768911

AT1G20330.1 122.084 73.7616 0.726932282 3.430653 0.0006021 0.023205388 0.023205388

AT5G43780.1 11.92649 34.7217 -3.42735 0.0006095 0.023457041 0.023457041

1.541668069

AT2G39030.1 47.57119 19.7472 1.26844001 3.41901 0.0006285 0.024121202 0.024121202

AT5G52650.1 205.7485 141.411 0.540987565 3.40929 0.0006513 0.024894302 0.024894302

AT2G39420.1 16.0851 41.2915 -3.405293 0.0006609 0.025192307 0.025192307

1.360119923

AT5G45350.1 46.3692 84.6594 -3.40234 0.0006681 0.025396589 0.025396589

0.868503428

AT5G27770.1 156.159 101.077 0.627560969 3.401497 0.0006702 0.0254403 0.0254403

AT3G15840.3 60.9621 103.882 -3.399224 0.0006758 0.025617694 0.025617694

0.768961189

AT1G43170.4 131.4465 81.5314 0.689048035 3.394298 0.000688 0.026047529 0.026047529

AT5G66040.1 68.09825 112.847 -3.382833 0.0007174 0.027122686 0.027122686

0.728678436

AT3G27820.1 97.56445 149.737 -3.375847 0.0007359 0.027745431 0.027745431

0.618003288

AT1G73230.1 166.4565 109.78 0.600529963 3.374315 0.00074 0.027862487 0.027862487

AT3G56800.1 116.46 172.815 -3.373603 0.0007419 0.027896916 0.027896916

0.569393922

AT3G48460.1 79.9663 42.6109 0.908169613 3.369323 0.0007535 0.028295515 0.028295515

AT1G56600.1 1.397526 13.5927 -3.366808 0.0007604 0.028516317 0.028516317

3.281885027

AT2G20340.1 23.1057 51.6196 -3.364646 0.0007664 0.028702055 0.028702055

1.159670165

AT1G20510.1 29.70575 61.168 -3.36164 0.0007748 0.028938347 0.028938347

1.042034892

AT1G04270.1 192.161 131.063 0.552055104 3.3568 0.0007885 0.029370886 0.029370886

AT3G05560.1 101.2684 58.7248 0.786142263 3.34746 0.0008156 0.030338135 0.030338135 AT3G11660.1 39.91865 75.1171 -3.338789 0.0008414 0.031217626 0.031217626

0.912078436

AT1G62510.1 35.51086 12.6655 1.487356268 3.338141 0.0008434 0.031248779 0.031242794

AT2G43150.1 54.35145 94.2755 -3.330066 0.0008683 0.032083904 0.032083904

0.794564373

AT3G26170.1 2.576655 16.2861 -2.66006988 -3.328902 0.0008719 0.032175513 0.032175513

AT5G64140.1 136.9705 86.7759 0.658498878 3.326939 0.0008781 0.032360099 0.03235192

AT5G56030.1 129.9964 81.2623 0.677813568 3.326273 0.0008802 0.03235192 0.03235192

AT3G13470.1 134.619 85.0349 0.662755067 3.317401 0.0009086 0.033309025 0.033309025

AT1G01100.3 130.5215 81.8099 0.67394013 3.315923 0.0009134 0.033441647 0.033441647

AT2G02100.1 63.984 31.6562 1.015223101 3.310465 0.0009314 0.034055811 0.034055811

AT3G09630.2 117.901 71.9835 0.711837799 3.309179 0.0009357 0.03416781 0.03416781

AT2G04080.1 19.606 45.7695 -3.303753 0.000954 0.034790286 0.034790286

1.223091303

12.57268

AT1G53903.1 34.7245 -3.302642 0.0009578 0.034882678 0.034882678

5 1.465661139

AT5G35970.1 139.274 198.837 -3.301443 0.0009619 0.034986348 0.034986348

0.513660284

AT5G01820.1 34.16445 66.6662 -3.294306 0.0009867 0.035840158 0.035840158

0.964459594

AT3G56710.1 4.43891 20.0737 -3.291057 0.0009981 0.036162187 0.036162187

2.177029197

12.45429

AT1G53885.1 34.3974 -3.287042 0.0010125 0.036634156 0.036595918

5 1.465656163

AT1G80180.1 23.4099 51.2345 -1.12999684 -3.283762 0.0010243 0.036966996 0.036966996

AT5G42820.2 0 10.9771 -3.282412 0.0010292 0.037048292 0.037048292

4.117051616

AT3G16530.1 7.604745 26.0176 -3.277516 0.0010472 0.037648114 0.037648114

1.774516109

AT4G34260.1 56.6728 26.8279 1.078922353 3.276341 0.0010516 0.037756421 0.037756421

AT2G 14750.1 118.724 173.646 -3.271452 0.00107 0.038365819 0.038365819

0.548537574

AT1G03400.1 19.945 45.9537 -3.270205 0.0010747 0.038436265 0.038436265

1.204153903

AT5G40670.1 29.15275 59.3368 -3.267641 0.0010845 0.038736322 0.038736322

1.025295145

19.47636

AT2G44120.2 4.15719 2.228043852 3.263361 0.001101 0.039225518 0.039225518

5

AT5G45350.2 38.6945 72.5102 -3.262278 0.0011052 0.0393253 0.0393253

0.906055435

AT1G01120.1 90.9571 51.8437 0.811017573 3.259839 0.0011148 0.039614304 0.039614304

AT4G15530.5 5.15112 21.2777 -3.258715 0.0011192 0.039720882 0.039709945

2.046384158

195.4151

AT1G52400.1 135.365 0.529687556 3.258432 0.0011203 0.039709945 0.039709945

5

AT4G14716.1 35.89045 68.5827 -0.93424469 -3.254552 0.0011357 0.040205107 0.040205107

AT3G44450.1 115.5999 169.527 -3.252518 0.0011439 0.040442575 0.040442575

0.552374915

11.15382

AT3G62650.1 31.9422 -1.51792514 -3.250299 0.0011528 0.040707778 0.040707778

5

AT3G51870.1 49.7047 22.2805 1.157600668 3.249221 0.0011572 0.040758893 0.040758893

10.03854

AT1G35140.1 0 4.891265253 3.24873 0.0011592 0.040777724 0.040760522

5

AT2G09990.1 107.949 64.9621 0.732679703 3.24849 0.0011602 0.040760522 0.040760522

AT4G09760.3 6.276375 23.3532 -3.247203 0.0011655 0.040893773 0.040893773

1.895616793

AT3G51430.1 5.86931 22.5797 -3.246511 0.0011683 0.040941633 0.040941633

1.943763504

AT2G01250.2 137.569 88.3278 0.639215924 3.246127 0.0011699 0.040945376 0.040945376

121.0834

AT1G54270.1 75.32 0.684896782 3.240195 0.0011945 0.041701854 0.041701854

5 AT5G64000.1 1.725425 13.6717 -3.234694 0.0012177 0.042407287 0.042407287

2.986168975

AT5G62210.1 15.54975 2.47134 2.653525974 3.227869 0.0012472 0.043377827 0.043348688

AT5G19860.1 28.31295 57.7088 -3.227704 0.0012479 0.043348688 0.043348688

1.027329257

AT3G52930.1 211.094 148.989 0.502679754 3.226243 0.0012543 0.043516159 0.043516159

AT2G36630.1 46.5118 20.3304 1.193958173 3.222202 0.0012721 0.044079693 0.044079693

AT2G39700.1 36.61115 13.9912 1.387763386 3.216242 0.0012988 0.044838068 0.044838068

AT5G67070.1 67.59125 35.0348 0.948047835 3.212666 0.0013151 0.045343821 0.045343821

AT1G56300.1 8.7324 27.5081 -3.210543 0.0013248 0.045567137 0.045567137

1.655406374

AT1G50290.1 10.86475 31.0838 -3.204137 0.0013547 0.046478454 0.046478454

1.516507906

AT4G29190.1 6.61898 23.6136 -3.194614 0.0014002 0.047921359 0.047921359

1.834937187

AT1G14150.2 30.24258 60.0252 -3.191125 0.0014172 0.048384815 0.048371258

0.988987084

118.6646

AT3G47520.1 74.0249 0.680807681 3.190535 0.0014201 0.048424258 0.048371258

5

AT2G26250.1 117.454 73.0736 0.684673657 3.190329 0.0014211 0.048399455 0.048371258

164.9534

AT1G75750.1 111.308 0.567501665 3.189874 0.0014234 0.048416525 0.048371258

5

AT5G59670.1 4.169245 18.8557 -3.189752 0.0014239 0.048377742 0.048371258

2.177142652

AT5G65207.1 7.281995 24.783 -3.189405 0.0014257 0.04837673 0.048371258

1.766945182

AT2G17230.1 35.16915 13.2435 1.409026018 3.189086 0.0014272 0.048371258 0.048371258

AT4G10120.1 20.8183 46.439 -3.186572 0.0014397 0.048734229 0.048734229

1.157484642

AT3G03470.1 8.907795 27.6019 -3.18314 0.0014569 0.04925572 0.04925572

1.631627317

AT2G39390.1 114.5075 70.8649 0.692298965 3.181367 0.0014658 0.049497978 0.049497978

ColxC24 at ZT15

MPV ColxC24 log2(Fold_c

Gene Name z-score p-value q-value q-value

RPKM RPKM hange)

AT2G01021.1 3461.741 740.071 2.225762199 43.95194 0 0 0

AT3G41768.1 2347.425 5409.73 -34.91497 4.41E-267 6.0643E-263 6.0643E-263

1.204477526

AT2G01020.1 1836.3 3497.17 -22.62845 2.27E-113 1.789E-109 1.5654E-109

0.929386154

AT3G41979.1 1836.3 3497.17 -22.62845 2.27E-113 1.789E-109 1.5654E-109

0.929386154

579.6276

AT2G14610.1 54.6289 3.407390069 22.36927 7.84E-111 4.3171E-107 4.3171E-107

22

ATCG00950.1 1088.383 2347.85 -21.48863 1.99E-102 7.8217E-99 7.8217E-99

1.109153912

AT3G21055.1 4251.64 2561.14 0.731233327 21.01078 5.23E-98 1.79836E-94 1.79836E-94

ATCG00920.1 815.312 1901.34 -20.90662 4.66E-97 1.42569E-93 1.42569E-93

1.221592385

AT2G21660.1 827.607 229.977 1.847456263 19.05715 5.73E-81 1.57767E-77 1.57767E-77

ATCG01210.1 1461.44 2681.08 -18.81474 5.72E-79 1.43111E-75 1.43111E-75

0.875423668

AT4G03520.2 1.001273 302.059 -8.23685118 -16.80823 2.12E-63 4.873E-60 4.873E-60

AT5G04140.2 242.664 6.41035 5.242413157 16.17897 7.10E-59 1.3955E-55 1.3955E-55

AT4G39260.2 441.755 100.806 2.131664953 15.27612 1.10E-52 1.78624E-49 1.78624E-49

ATCGOl 180.1 1541.505 2511.18 -15.02716 4.87E-51 7.06184E-48 7.06184E-48

0.704025875

AT1G52400.1 59.40885 348.168 -14.91604 2.59E-50 3.56742E-47 3.56742E-47

2.551033844 AT1G52400.2 47.99325 278 -13.27849 3.09E-M) 3.86048E-37 3.86048E-37

2.534181465

179.4729

AT1G15690.2 0 8.617609432 12.73053 4.00E-37 4.78857E-34 4.78857E-34

9

AT4G25100.2 371.2835 790.044 -12.27507 1.23E-34 1.41409E-31 1.41409E-31

1.089411801

AT4G10340.1 1469.24 2189.77 -11.68032 1.61E-31 1.76939E-28 1.76939E-28

0.575709267

AT4G25100.4 140.914 8.91135 3.983027137 11.6611 2.01E-31 2.13247E-28 2.13247E-28

AT1G75750.1 359.5155 116.444 1.626417668 11.49928 1.33E-30 1.35613E-27 1.35613E-27

277.5745

AT5G24770.1 73.8251 1.910691909 11.28535 1.55E-29 1.524E-26 1.524E-26

35

266.8912

AT1G75040.1 73.4331 1.861749597 10.87643 1.49E-27 1.32574E-24 1.32574E-24

5

AT1G29920.1 164.5345 423.093 -10.74136 6.51E-27 5.59826E-24 5.59826E-24

1.362584693

AT3G12120.2 4.96863 112.235 -10.67994 1.26E-26 1.05392E-23 1.05392E-23

4.497530722

372.5500

AT5G55450.1 140.887 1.40289576 10.5099 7.78E-26 6.29662E-23 6.29662E-23

5

AT5G04140.1 238.819 522.786 -10.29052 7.78E-25 5.94562E-22 5.94562E-22

1.130302888

ATCG00020.1 73.8648 253.993 -10.1438 3.53E-24 2.62681E-21 2.62681E-21

1.781829815

AT1G67870.1 629.7125 330.548 0.92983352 9.879421 5.11E-23 3.70363E-20 3.70363E-20

AT2G44120.2 83.07385 2.25871 5.200823459 9.459184 3.10E-21 2.13578E-18 2.13578E-18

AT3G28220.1 28.57905 148.045 -9.327008 1.09E-20 7.31156E-18 7.31156E-18

2.373005901

AT1G78370.1 854.062 1292.31 -9.28234 1.66E-20 1.08664E-17 1.08664E-17

0.597539476

186.4137

AT5G24770.2 49.8654 1.902396975 9.221482 2.93E-20 1.87581E-17 1.87581E-17

145

AT5G45775.1 75.92565 0 7.00967632 8.902619 5.45E-19 3.41237E-16 3.41237E-16

AT3G29240.1 79.69635 5.42383 3.877129786 8.695311 3.46E-18 2.11579E-15 2.11579E-15

20.71277

AT1G52040.1 110.992 -8.173564 2.99E-16 1.75359E-13 1.75359E-13

225 2.421863129

AT2G21660.2 701.7705 437.244 0.682560716 8.033215 9.50E-16 5.33411E-13 5.33411E-13

AT1G19670.1 126.0645 286.49 -7.911162 2.55E-15 1.34987E-12 1.34987E-12

1.184322715

ATCG00490.1 47.7807 157.021 -7.768115 7.97E-15 4.06087E-12 4.06087E-12

1.716457623

AT2G03680.2 0 57.9074 -7.753177 8.96E-15 4.48548E-12 4.48548E-12

6.973220575

144.9084

AT1G51402.1 43.6022 1.73266889 7.631221 2.33E-14 1.12301E-11 1.12301E-11

5

AT3G57260.1 121.8981 31.1592 1.967945541 7.624789 2.44E-14 1.16009E-11 1.16009E-11

AT3G27830.1 941.4945 649.667 0.535252242 7.526437 5.21E-14 2.39227E-11 2.36508E-11

AT1G52000.1 26.67185 113.168 -7.525771 5.24E-14 2.36508E-11 2.36508E-11

2.085076271

AT1G21600.2 52.3702 0 6.707250432 7.456062 8.91E-14 3.89514E-11 3.89514E-11

51.58478

AT3G18410.2 0 6.542466115 7.427602 1.11E-13 4.75632E-11 4.75632E-11

5

176.4607

AT5G23820.1 66.774 1.401988951 7.229206 4.86E-13 2.02627E-10 2.02627E-10

5

481.2081

AT5G24780.1 291.397 0.723674992 6.994223 2.67E-12 1.04891E-09 1.04891E-09

3

AT2G24850.1 21.3537 92.0221 -2.10749431 -6.833139 8.31E-12 3.17618E-09 3.17618E-09

AT1G48450.2 0 43.55 -6.782036 1.18E-11 4.4682E-09 4.4682E-09

6.641633403

AT5G64040.1 493.4495 735.338 -6.761753 1.36E-11 5.07136E-09 5.07136E-09

0.575505095

AT5G54270.1 415.562 638.195 -6.734946 1.64E-11 6.0189E-09 6.0189E-09

0.618933569 AT1G57720.2 45.49585 2.63292 4.110999361 6.688051 2.26E-11 7.98147E-09 7.90264E-09

42.09787

AT4G33510.2 0 6.690615795 6.68764 2.27E-11 7.90264E-09 7.90264E-09

15

AT2G 18660.1 91.00222 23.0413 1.981679624 6.617859 3.64E-11 1.2385E-08 1.2385E-08

AT5G36700.2 78.6425 17.9609 2.130449541 6.442481 1.18E-10 3.94562E-08 3.94562E-08

35.75408

AT1G64770.2 0 5.853867161 6.235626 4.50E-10 1.49235E-07 1.4799E-07

2

AT5G10760.1 124.5697 45.5232 1.452279315 6.235062 4.52E-10 1.4799E-07 1.4799E-07

73.26946

AT4G05050.3 170.032 -6.218984 5.00E-10 1.62048E-07 1.62048E-07

85 1.214522231

AT4G21105.2 35.6223 0 6.822769709 6.13106 8.73E-10 2.79417E-07 2.79417E-07

AT4G38680.1 162.705 73.0272 1.155752765 5.984206 2.17E-09 6.88011E-07 6.88011E-07

AT3G02360.2 32.61275 0 5.464165053 5.948284 2.71E-09 8.47605E-07 8.47605E-07

AT1G35720.1 152.038 275.793 -5.93116 3.01E-09 9.30356E-07 9.30356E-07

0.859153887

AT4G39260.4 131.9026 53.2971 1.307344062 5.928667 3.05E-09 9.34097E-07 9.34097E-07

AT5G59890.2 32.99025 0 6.706697438 5.917873 3.26E-09 9.86532E-07 9.86532E-07

148.5108

AT4G08870.1 64.333 1.20693745 5.914743 3.32E-09 9.94543E-07 9.94543E-07

485

AT5G13710.2 3.22441 38.2966 -5.810886 6.21E-09 1.83937E-06 1.83937E-06

3.570109208

AT2G20630.2 30.10265 0 6.53991854 5.674303 1.39E-08 4.035E-06 4.035E-06

AT2G41840.1 536.4915 372.104 0.527849415 5.61097 2.01E-08 5.70961E-06 5.70961E-06

86.86759

AT4G17470.1 28.6921 1.598164532 5.581894 2.38E-08 6.68268E-06 6.68268E-06

5

AT2G38170.3 124.6885 52.7325 1.241564111 5.539995 3.02E-08 8.32638E-06 8.32638E-06

AT3G22121.1 290.8145 177.171 0.714956726 5.380644 7.42E-08 1.98354E-05 1.98354E-05

AT5G10140.2 0 27.2907 -5.374929 7.66E-08 2.00849E-05 2.00849E-05

5.048308192

AT5G64040.2 344.6735 503.351 -5.330256 9.81E-08 2.54688E-05 2.54688E-05

0.546334398

AT3G01910.2 2.40797 31.1999 -5.3132 1.08E-07 2.77114E-05 2.77114E-05

3.695652082

322.5456

AT5G24780.2 205.103 0.653154811 5.241832 1.59E-07 3.97865E-05 3.97865E-05

6

AT1G20620.4 152.5625 260.288 -5.23898 1.61E-07 4.00422E-05 4.00422E-05

0.770708411

174.5131

AT2G30766.1 91.3746 0.933470661 5.216624 1.82E-07 4.47838E-05 4.47838E-05

5

AT2G38210.1 62.0906 17.464 1.829990838 5.185346 2.16E-07 5.2524E-05 5.2524E-05

AT1G14150.1 219.2235 127.218 0.785099647 5.059368 4.21E-07 0.000100689 0.000100689

AT4G13510.1 200.952 113.858 0.819615273 5.023092 5.08E-07 0.000120659 0.000120659

AT5G03240.2 64.52 20.0866 1.683513055 4.990942 6.01E-07 0.000140167 0.000140167

28.29400

AT5G44570.2 2.67128 3.404893395 4.940297 7.80E-07 0.000180437 0.000180437

7

AT3G12120.1 188.835 106.828 0.821836357 4.880372 1.06E-06 0.000240886 0.000240886

AT1G32550.2 0 22.1078 -4.8707 1.11E-06 0.00025091 0.00025091

6.166385615

AT5G42800.1 22.2941 0 6.688338482 4.866998 1.13E-06 0.000253576 0.000253576

AT3G57240.1 66.74325 22.6734 1.557621192 4.803309 1.56E-06 0.000338267 0.000338267

AT5G17220.1 29.34973 3.56225 3.042486557 4.789752 1.67E-06 0.000359115 0.000359115

AT4G35090.1 226.3215 342.333 -4.77159 1.83E-06 0.000390025 0.000390025

0.597026727

AT2G27450.2 0 20.9109 -4.746875 2.07E-06 0.000437433 0.000437433

5.747751624

151.0342

AT2G46390.1 80.7414 0.903495239 4.723862 2.31E-06 0.000486256 0.000486256

5 AT2G03680.1 164.3215 90.8615 0.854780229 4.705126 2.54E-06 0.000529081 0.000529081

AT4G00810.2 136.1445 70.4949 0.949547908 4.672714 2.97E-06 0.000597247 0.000597247

AT3G05730.1 140.0293 230.546 -4.637016 3.53E-06 0.000700009 0.000700009

0.719325904

AT1G14250.1 70.0527 136.677 -4.609376 4.04E-06 0.000794113 0.000794113

0.964257925

AT5G02870.2 259.953 167.121 0.637357775 4.606789 4.09E-06 0.000798352 0.000798352

110.0902

AT1G58380.1 53.2725 1.047223813 4.550276 5.36E-06 0.001038576 0.001038576

5

46.57989

AT3G61440.2 101.752 -4.529241 5.92E-06 0.001139501 0.001139501

55 1.127277844

AT1G67865.1 185.8609 109.934 0.757585652 4.519462 6.20E-06 0.001185131 0.001185131

AT1G01100.4 219.142 136.453 0.683461899 4.493179 7.02E-06 0.001332074 0.001326561

AT5G28050.2 19.81015 0 7.383785204 4.489005 7.16E-06 0.001339958 0.001339958

24.42635

AT5G44570.1 2.61547 3.223296202 4.485342 7.28E-06 0.001353977 0.001353977

05

ATCG00960.1 16.9773 53.9485 -1.66797581 -4.460843 8.16E-06 0.001508222 0.001508222

AT1G11850.2 48.99665 14.8955 1.717806561 4.410865 1.03E-05 0.001876916 0.001876916

AT4G25100.3 169.1335 261.687 -4.380322 1.19E-05 0.002118219 0.002118219

0.629679816

ATCGOl 170.1 15.46675 50.2841 -4.367452 1.26E-05 0.002218116 0.002218116

1.700932209

17.81928

AT3G08030.2 0 4.996221369 4.360917 1.30E-05 0.002270866 0.002270866

35

AT5G02870.1 286.834 193.918 0.56476932 4.353997 1.34E-05 0.002328946 0.002320244

17.72738

AT4G25700.2 0 4.460762996 4.262471 2.02E-05 0.003435422 0.003435422

6

AT3G29240.2 79.5884 143.836 -4.2599 2.05E-05 0.003453845 0.003453845

0.853794727

33.75093

AT4G35450.4 78.587 -4.241283 2.22E-05 0.003730333 0.003730333

5 1.219363209

AT1G52410.2 7.791805 34.2854 -4.208733 2.57E-05 0.004258509 0.004258509

2.137564876

AT3G51370.2 0 17.2424 -4.156014 3.24E-05 0.005152924 0.005152924

4.362322625

AT5G54060.1 15.7555 0 6.322329217 4.121326 3.77E-05 0.005925353 0.005925353

AT3G51860.1 21.68511 2.64867 3.033364748 4.111302 3.93E-05 0.006153442 0.006131293

AT4G00810.1 112.4418 59.8053 0.910833206 4.103223 4.07E-05 0.006300804 0.006300804

15.87751

AT5G63890.1 0 6.838229056 4.091504 4.29E-05 0.006554249 0.006554249

8

AT1G71880.1 80.0928 37.3041 1.102338354 4.043931 5.26E-05 0.007993839 0.007993839

29.80730

AT3G02560.2 69.3476 -3.98106 6.86E-05 0.010099395 0.010099395

95 1.218179795

AT4G38550.1 190.9064 122.887 0.635533165 3.937909 8.22E-05 0.011908308 0.011883592

14.35398

AT4G22880.1 0 6.268462434 3.936887 8.25E-05 0.011896496 0.011883592

75

AT3G02360.1 35.13195 76.5535 -3.918314 8.92E-05 0.012652575 0.012599111

1.123684683

AT3G01290.1 105.2984 56.8883 0.88827964 3.88903 0.0001006 0.014063293 0.014052751

13.98048

AT4G22880.2 0 6.217696855 3.887983 0.0001011 0.014052751 0.014052751

2

AT5G03240.3 95.47145 158.007 -3.874554 0.0001068 0.014629036 0.014629036

0.726847198

AT3G16570.1 142.124 85.2785 0.736896231 3.859801 0.0001135 0.015387947 0.015387947

AT4G35090.2 158.616 236.357 -3.832175 0.000127 0.017055405 0.017055405

0.575429286

AT2G44290.1 90.8008 47.1084 0.946720676 3.806641 0.0001409 0.018642535 0.018642535

AT4G01080.1 87.086 145.92 -3.804639 0.000142 0.018704056 0.018704056

0.744664921 AT5G45775.2 89.9534 149.384 -3.790127 0.0001506 0.019736916 0.019736916

0.731775917

123.8201

AT2G47840.1 72.2454 0.777268476 3.769992 0.0001633 0.021297916 0.021297916

5

AT3G07390.1 130.1808 77.7734 0.74316797 3.72087 0.0001985 0.025069472 0.025069472

AT1G21500.1 221.5205 151.709 0.54613354 3.714884 0.0002033 0.025553215 0.025553215

AT2G02100.1 80.13995 135.131 -3.701001 0.0002148 0.026870225 0.026870225

0.753765162

AT1G52410.1 6.19633 26.7843 -3.691373 0.000223 0.027532731 0.027532731

2.111901707

AT2G44120.1 81.46215 136.601 -3.685972 0.0002278 0.027997857 0.027997857

0.745766249

21.42631

AT2G37600.2 3.85465 2.474711974 3.676655 0.0002363 0.028783058 0.028783058

5

AT5G36790.2 169.03 245.09 -3.653566 0.0002586 0.031224236 0.031224236

0.536032298

AT5G59890.1 53.3122 98.6382 -3.648388 0.0002639 0.031721057 0.031721057

0.887680755

20.27753

AT4G 14090.1 3.52182 2.525489204 3.617748 0.0002972 0.035413012 0.035413012

5

AT1G14880.1 176.1683 116.273 0.599438222 3.594901 0.0003245 0.03833878 0.03833878

AT2G41430.2 42.9403 83.4118 -3.58072 0.0003426 0.040308149 0.040308149

0.957919221

AT1G21880.1 11.74495 0 5.964335555 3.572144 0.0003541 0.041474426 0.041377303

AT2G34430.1 61.06315 107.97 -3.571646 0.0003547 0.041377303 0.041377303

0.822256589

17.41794

AT2G23130.2 2.45794 2.825052818 3.558923 0.0003724 0.043069117 0.043069117

5

AT3G23450.1 96.7361 54.0827 0.838887198 3.553693 0.0003799 0.04375096 0.04375096

AT5G47700.2 120.455 72.6856 0.7287528 3.519769 0.0004319 0.049334227 0.049334227

C24xCol at ZT15

MPV C24xCol log2(Fold_c

Gene Name z-score p-value q-value q-value

RPKM RPKM hange)

AT2G01021.1 3461.741 607.577 2.510358627 46.93695 0 0 0

579.6276

AT2G14610.1 35.2795 4.038224328 23.68328 5.36E-124 7.3615E-120 7.3615E-120

22

481.2081

AT5G24780.1 12.4192 5.276016869 22.74701 1.54E-114 1.4061E-110 1.4061E-110

3

322.5456

AT5G24780.2 9.00857 5.162061481 18.58423 4.31E-77 1.97312E-73 1.97312E-73

6

AT2G21660.1 827.607 247.085 1.743938417 18.2042 4.78E-74 1.87492E-70 1.87492E-70

277.5745

AT5G24770.1 5.10647 5.76440508 17.34266 2.24E-67 7.69234E-64 7.69234E-64

35

AT4G03520.2 1.001273 285.747 -16.43798 1.02E-60 3.12034E-57 3.12034E-57

8.156759159

ATCG00950.1 1088.383 1946.32 -0.8385626 -15.65182 3.23E-55 8.8652E-52 8.8652E-52

186.4137

AT5G24770.2 3.30768 5.816544525 14.21173 7.75E^6 1.93436E-42 1.93436E-42

145

AT1G75750.1 359.5155 76.2046 2.23810398 14.12814 2.55E^5 5.83034E-42 5.83034E-42

AT4G39260.2 441.755 119.526 1.885921988 14.03009 1.02E^4 2.15481E-41 2.15481E-41

ATCG01210.1 1461.44 2288.02 -13.52237 1.15E^1 2.26327E-38 2.26327E-38

0.646709063

AT3G41768.1 2347.425 3369.09 -13.51355 1.30E^1 2.38144E-38 2.38144E-38

0.521279904

AT4G25100.4 140.914 6.20682 4.504816834 12.01334 3.02E-33 3.95408E-30 3.95408E-30

144.9084

AT1G51402.1 9.8557 3.878039572 11.69162 1.41E-31 1.7559E-28 1.7559E-28

5

AT4G 18440.1 404.0195 147.769 1.451081284 11.13576 8.40E-29 9.22919E-26 9.22919E-26

AT2G23672.1 17.0232 152.207 -11.04433 2.34E-28 2.46617E-25 2.46617E-25

3.160460546 AT1G29920.1 164.5345 406.653 -10.27441 9.19E-25 9.01279E-22 9.01279E-22

1.305408134

266.8912

AT1G75040.1 81.0298 1.719727523 10.23549 1.37E-24 1.3019E-21 1.3019E-21

5

AT1G65960.1 109.535 0 8.251457359 10.13331 3.93E-24 3.59818E-21 3.59818E-21

116.0679

AT4G15210.1 10.7609 3.431099004 10.00396 1.46E-23 1.29693E-20 1.29693E-20

5

AT3G62030.2 328.8595 620.297 -0.9154878 -9.515474 1.81E-21 1.55218E-18 1.55218E-18

148.5108

AT4G08870.1 28.6892 2.371988677 9.401832 5.36E-21 4.46176E-18 4.46176E-18

485

AT2G44120.2 83.07385 2.58449 5.006442891 9.397117 5.61E-21 4.52898E-18 4.52898E-18

372.5500

AT5G55450.1 159.547 1.223452775 9.381228 6.52E-21 5.11589E-18 5.11589E-18

5

AT2G23670.1 394.7805 175.692 1.168002229 9.309295 1.29E-20 9.81535E-18 9.81535E-18

86.86759

AT4G17470.1 5.15348 4.075199218 9.192622 3.83E-20 2.84521E-17 2.84521E-17

5

AT5G45775.1 75.92565 0 6.665457351 8.971287 2.93E-19 2.11774E-16 2.11774E-16

AT4G25100.2 371.2835 657.635 -0.82476588 -8.964454 3.12E-19 2.19547E-16 2.19547E-16

94.03327

AT4G15210.2 9.03151 3.380132244 8.949431 3.57E-19 2.4529E-16 2.4529E-16

5

176.4607

AT5G23820.1 47.8109 1.883935853 8.860369 7.97E-19 5.21394E-16 5.21394E-16

5

AT3G09820.2 0 88.0258 -8.835747 9.94E-19 6.34953E-16 6.34953E-16

8.740960313

73.26946

AT4G05050.3 0 7.726125357 8.49983 1.90E-17 1.18491E-14 1.18491E-14

85

65.87779

AT4G16590.1 2.00549 5.037765533 8.374935 5.53E-17 3.29833E-14 3.29833E-14

24

AT4G21960.1 799.3895 1160.58 -8.152787 3.56E-16 2.07772E-13 2.07772E-13

0.537875444

AT2G21660.2 701.7705 432.971 0.696728905 8.045428 8.59E-16 4.81621E-13 4.81621E-13

AT3G22235.2 810.328 1164.88 -7.969981 1.59E-15 8.71538E-13 8.71538E-13

0.523603446

AT5G36700.2 78.6425 10.5243 2.901584906 7.633889 2.28E-14 1.15824E-11 1.15824E-11

AT2G 18660.1 91.00222 17.0511 2.416036927 7.43912 1.01E-13 5.06036E-11 5.06036E-11

51.58478

AT3G18410.2 0 6.575845369 7.40949 1.27E-13 6.21683E-11 6.21683E-11

5

46.57989

AT3G61440.2 0 6.23926614 7.081849 1.42E-12 6.62012E- 10 6.62012E-10

55

AT1G57720.2 45.49585 0 5.71178973 7.020741 2.21E-12 9.46883E-10 9.46883E-10

AT3G57260.1 121.8981 36.448 1.741764082 6.978854 2.98E-12 1.25719E-09 1.25719E-09

179.4729

AT1G15690.2 334.67 -6.881843 5.91E-12 2.45813E-09 2.45813E-09

9 0.898972493

42.09787

AT4G33510.2 0 6.678861795 6.678103 2.42E-11 9.92025E-09 9.92025E-09

15

AT1G48450.2 0 39.4203 -6.49789944 -6.483079 8.99E-11 3.52532E-08 3.52532E-08

AT1G65960.2 137.2315 264.127 -6.37406 1.84E-10 7.02066E-08 7.02066E-08

0.944620112

1.032503

AT3G47370.3 37.8044 -5.19433558 -6.360863 2.01E-10 7.54648E-08 7.54648E-08

5

AT2G38170.3 124.6885 44.3047 1.492796754 6.31743 2.66E-10 9.86852E-08 9.86852E-08

AT1G14250.1 70.0527 14.6331 2.259205222 6.271747 3.57E-10 1.30712E-07 1.30712E-07

AT2G34430.1 61.06315 149.081 -6.154774 7.52E-10 2.64679E-07 2.64679E-07

1.287722482

35.75408

AT1G64770.2 0 6.87708154 6.123021 9.18E-10 3.19142E-07 3.19142E-07

2

AT4G39260.4 131.9026 51.4437 1.358406688 6.052883 1.42E-09 4.88344E-07 4.88344E-07

33.75093

AT4G35450.4 0 5.762038408 6.047285 1.47E-09 4.99371E-07 4.99371E-07

5

AT5G59890.2 32.99025 0 7.013572396 5.85809 4.68E-09 1.49498E-06 1.49498E-06 AT5G10760.1 124.5697 50.3447 1.307041381 5.71076 1.12E-08 3.50954E-06 3.50954E-06

183.9688

AT1G45201.1 90.7849 1.018937244 5.690093 1.27E-08 3.91739E-06 3.91739E-06

5

29.80730

AT3G02560.2 0 5.205813557 5.654176 1.57E-08 4.72524E-06 4.69436E-06

95

AT5G13710.2 3.22441 36.5534 -5.653425 1.57E-08 4.69436E-06 4.69436E-06

3.502898498

AT5G10140.2 0 29.5086 -5.616044 1.95E-08 5.76871E-06 5.76871E-06

5.161034268

28.29400

AT5G44570.2 1.04258 4.762266511 5.442241 5.26E-08 1.50494E-05 1.50494E-05

7

AT3G01290.1 105.2984 40.7935 1.368072317 5.437373 5.41E-08 1.53068E-05 1.53068E-05

AT4G39800.1 225.2965 355.253 -5.39912 6.70E-08 1.87642E-05 1.87642E-05

0.657021931

AT4G35090.1 226.3215 355.086 -5.344967 9.04E-08 2.45863E-05 2.45863E-05

0.649794837

AT1G02930.1 49.2991 10.0629 2.292515178 5.308071 1.11E-07 2.98257E-05 2.98257E-05

AT1G14150.2 58.98955 130.729 -5.263124 1.42E-07 3.73938E-05 3.73938E-05

1.148047905

AT5G17220.1 29.34973 2.11703 3.793233613 5.222849 1.76E-07 4.60765E-05 4.60765E-05

AT1G35720.1 152.038 253.141 -0.73550924 -5.034674 4.79E-07 0.000120584 0.000120584

AT4G38680.1 162.705 84.9059 0.938321873 4.998005 5.79E-07 0.000143298 0.000143298

AT2G38210.1 62.0906 18.9262 1.713990097 4.925026 8.43E-07 0.000206798 0.000206798

AT2G24200.2 22.4597 68.3165 -4.916452 8.81E-07 0.000214148 0.000214148

1.604895407

280.9648

AT4G02520.1 177.338 0.663887944 4.87827 1.07E-06 0.000257778 0.000257778

5

24.42635

AT5G44570.1 1.46219 4.062235634 4.869996 1.12E-06 0.000266473 0.000266473

05

AT3G57240.1 66.74325 22.7349 1.553713281 4.759734 1.94E-06 0.000443573 0.000443573

20.51970

AT2G38530.1 0 5.215145096 4.692111 2.70E-06 0.000613629 0.000613629

6

AT4G13510.1 200.952 120.855 0.733573775 4.502328 6.72E-06 0.001441881 0.001441881

AT5G28050.2 19.81015 0 7.364767124 4.485284 7.28E-06 0.001549981 0.001549981

AT5G02870.2 259.953 169.359 0.618166149 4.404957 1.06E-05 0.002217782 0.002217782

17.72738

AT4G25700.2 0 5.315375935 4.368436 1.25E-05 0.00260319 0.00260319

6

AT3G49260.2 0 17.4991 -4.334143 1.46E-05 0.003021083 0.003021083

5.278782217

AT5G63530.2 5.379785 30.0142 -4.329184 1.50E-05 0.003066864 0.003066864

2.480024792

14.77304

AT1G29395.1 48.1886 -4.312881 1.61E-05 0.00322976 0.00322976

5 1.705724665

ATCG00020.1 73.8648 135.819 -4.299784 1.71E-05 0.003401818 0.003401818

0.878726393

178.5347

AT1G19570.1 107.192 0.73600767 4.255974 2.08E-05 0.004111749 0.004111749

5

AT5G42080.2 16.58675 0 6.003075889 4.235929 2.28E-05 0.004464218 0.004464218

AT5G42800.1 22.2941 0 9.52397146 4.232278 2.31E-05 0.004505159 0.004505159

AT3G51370.2 0 17.6987 -4.230426 2.33E-05 0.004510415 0.004510415

4.400005421

AT5G02870.1 286.834 195.468 0.553283601 4.190086 2.79E-05 0.005280625 0.005280625

AT4G35090.2 158.616 241.98 -4.165641 3.10E-05 0.005839336 0.005839336

0.609349505

110.0902

AT1G58380.1 57.2074 0.944413022 4.133389 3.57E-05 0.006677079 0.006677079

5

103.2391

AT3G47370.1 53.0319 0.961057826 4.061347 4.88E-05 0.008931575 0.008931575

5

141.3817

AT1G19570.2 81.9168 0.78736464 4.014861 5.95E-05 0.0107453 0.010688668

5

AT1G01100.4 219.142 143.304 0.612787133 4.013024 5.99E-05 0.010688668 0.010688668 174.5131

AT2G30766.1 107.857 0.69421594 3.998407 6.38E-05 0.011224807 0.011224807

5

AT1G48860.2 14.66555 0 5.821620651 3.986099 6.72E-05 0.011747639 0.011747639

AT1G14150.1 219.2235 144.649 0.599846109 3.938133 8.21E-05 0.014271377 0.014271377

AT2G41430.2 42.9403 87.4553 -3.929085 8.53E-05 0.014725906 0.014725906

1.026213547

AT1G71880.1 80.0928 38.3209 1.063541112 3.888089 0.000101 0.017339773 0.017339773

AT3G09820.1 243.1455 165.176 0.557815812 3.88615 0.0001018 0.017370218 0.017370218

AT3G27690.1 25.05365 60.1201 -3.849258 0.0001185 0.019958518 0.019958518

1.262826608

AT1G11850.2 48.99665 18.5258 1.403147269 3.780852 0.0001563 0.025698428 0.025698428

AT5G17310.1 13.114 0 5.554890206 3.76686 0.0001653 0.026860085 0.026860085

14.35398

AT4G22880.1 0 7.777263739 3.753615 0.0001743 0.028154003 0.028154003

75

AT5G45775.2 89.9534 147.158 -3.721923 0.0001977 0.031381295 0.031381295

0.710116256

AT1G10830.2 0 14.5844 -3.716528 0.000202 0.031874446 0.031874446

3.915779711

13.98048

AT4G22880.2 0 7.727703228 3.712604 0.0002051 0.032187827 0.032187827

2

AT2G37600.1 92.2382 48.6732 0.922236732 3.709009 0.0002081 0.032462785 0.032462785

20.27753

AT4G 14090.1 3.20293 2.662418111 3.707569 0.0002093 0.032463376 0.032463376

5

AT3G18140.2 2.04307 17.237 -3.670891 0.0002417 0.037286582 0.037167676

3.076698164

AT1G48450.1 96.50765 52.3755 0.881751195 3.653276 0.0002589 0.039279407 0.039279407

AT5G54060.1 15.7555 0 9.157965058 3.645229 0.0002672 0.040306398 0.040306398

AT2G45560.2 1.283521 14.9864 -3.636978 0.0002759 0.041391823 0.041391823

3.545475058

AT2G38310.1 54.4937 23.1272 1.23649884 3.61747 0.0002975 0.044156643 0.044156643

ATCGOl 170.1 15.46675 42.3211 -3.594794 0.0003246 0.047671263 0.047671263

1.452207048

AT2G41410.1 76.9253 38.7775 0.98823837 3.587706 0.0003336 0.048725047 0.048725047

AT5G36790.2 169.03 241.713 -3.580788 0.0003426 0.049768973 0.049734862

0.516015744

AT2G43745.1 0 11.8812 -3.579588 0.0003441 0.049734862 0.049734862

6.102440347

Table 7. Biotic, abiotic, and photosynthesis gene expression level, location of (evening element) EE and (CCAl-bininding sequence) CBS motifs, and circadian correlations in hybrids and parents.

Table 8. Results of regression analysis performed using JMP10

Claims

1. A method of producing a hybrid plant with increased biomass comprising the steps of:

(a) detecting an expression level of a stress-responsive gene in a plurality of plants;

(b) selecting a first parent plant having a first expression level of the stress-responsive gene from the plurality of plants;

(c) selecting a second parent plant of a different genotype having a second expression level of the stress-responsive gene from the plurality of plants, wherein the second expression level is different from the first expression level; and

(d) crossing the first parent plant with the second parent plant to produce a progeny plant.

2. The method of claim 1 wherein the stress-responsive gene is an abiotic stress gene.

3. The method of claim 2, wherein the stress-responsive gene is selected from the group consisting of: LSR3, ERD11/GSTF6, COR47/RD17, LTI45/ERD10, COR413-TM1, ALDH7B4, ATGRP3, PIP2B, RD28, CAX1/RCI4, COR15A, PIP1B, LTI30, PIP1A, PIP1;4, COR78/RD29A, COR6.6/KIN2, RD22, ERDl, GRP8, GRP2, GRP7/CCR2, MDH, HSP70 family, CAT2, LOS2, ATAVPl, LTP4, TCH2, TCH4, mtHsc70-l, COR15B, MTIA, LTP3, LOS1, Salt-stress responsive protein, FIB, KIN1, and homologs thereof.

4. The method of claim 3, wherein the stress-responsive gene is selected from the group consisting of: COR47/RD17, COR78/RD29A, and homologs thereof.

5. The method of claim 1, wherein the stress-responsive gene is a biotic stress gene.

6. The method of claim 5, wherein the stress-responsive gene is selected from the group consisting of: PR1, PR5, NUDT6, HSPR02, CNGC11, EBP/ERF72, PCC1, HR3/MLA10, CAX3, PAD4, WRKY70, PR2/BGL2, ACD6, NIA2, NHL3, Protease inhibitor, WAKl, WAK2, EP1, RVE2, BG3, LURP1, DMR6, SAG21, SUR2, ANK, and homologs thereof.

7. The method of claim 6, wherein the stress-responsive gene is selected from the group consisting of: PR1, ACD6, and homologs thereof.

8. The method of claim 1 wherein the step of detecting an expression level of a stress- responsive gene is performed at one or more time points selected from the group consisting of zeitgeber time (ZT) 0, ZT6, ZT12, ZT15, ZT18, and ZT21.

9. The method of claim 1, wherein the ratio of the first gene expression level to the second gene expression level is greater than 1.00.

10. The method of claim 9, wherein the ratio of the first gene expression level to the second gene expression level is greater than 1.5.

11. The method of claim 10, wherein the ratio of the first gene expression level to the second gene expression level is greater than 3.0.

12. The method of claim 1, wherein the expression levels of stress-responsive genes are detected using quantitative reverse transcription polymerase chain reaction (qRT-PCR).

13. The method of claim 1, wherein the progeny plant exhibits and improved agronomic trait compared with the parent plants.

14. The method of claim 13, wherein the agronomic trait is selected from the group consisting of: biomass, yield, disease tolerance, insect resistance, pathogen resistance, plant growth and development, starch content, oil content, fatty acid content, protein content, fruit ripening, and stress resistance.

15. The method of claim 1, wherein the plants are monocot plants.

16. The method of claim 1, wherein the plants are dicot plants.

17. The method of claim 1, wherein the plant type of the plants is selected from the group consisting of: Arabidopsis thaliana, maize (corn; Zea mays), soybean (Glycine max), cotton (Gossypium hirsutum; Gossypium sp.), peanut (Arachis hypogaea), barley (Hordeum vulgare); oats (Avena sativa); orchard grass (Dactylis glomerata); rice (Oryza sativa, including indica and japonica varieties); sorghum (Sorghum bicolor); sugar cane (Saccharum sp.); tall fescue (Festuca arundinacea); turfgrass species (e.g. species: Agrostis stolonifera, Poa pratensis, Stenotaphrum secundatum); wheat (Triticum aestivum); alfalfa (Medicago sativa); members of the genus Brassica, including broccoli, cabbage, carrot, cauliflower, Chinese cabbage; cucumber, dry bean and other leguminous plants, eggplant, tobacco (Nicotiana sp.), fennel, garden beans, gourd, leek, lettuce, melon, okra, onion, pea, pepper, pumpkin, radish, spinach, squash, sweet corn, tomato, potato, watermelon, Miscanthus, ornamental plants, and other fruit, vegetable, tuber, oilseed, and root crops, wherein oilseed crops may include soybean, canola, oil seed rape, oil palm, sunflower, olive, corn, cottonseed, peanut, flaxseed, safflower, and coconut.

18. A progeny plant produced by the method of claim 1.

19. A plant part, plant cell, or seed of the plant of claim 18.

20. A method of producing a hybrid plant with increased biomass comprising the steps of:

(a) detecting an expression level of at least two stress-responsive genes in a plurality of plants;

(b) selecting a first parent plant having a first expression level of each of the stress- responsive genes from the population of plants;

(c) selecting a second parent plant of a different genotype having a second expression level of each of the stress-responsive gene from the population of plants, wherein the first expression level for each gene is different than the second expression level for that gene; and

(d) crossing the first parent plant with the second parent plant to produce a progeny plant.