WO2016188096A1

WO2016188096A1 - A mixing device

Info

Publication number: WO2016188096A1
Application number: PCT/CN2015/097374
Authority: WO
Inventors: Jianfeng Wang; Haipeng Hu
Original assignee: ABON Biopharm Hangzhou Co Ltd
Current assignee: ABON Biopharm Hangzhou Co Ltd
Priority date: 2015-05-27
Filing date: 2015-12-15
Publication date: 2016-12-01
Anticipated expiration: 2017-11-27

Abstract

A kind of mixing device (100), used for mixing liquid or solid, comprises a device body (130) and a cover body covering and sealing the device body (130). The cover body comprises a first cover body (110) and a second cover body (120); and the first cover body (110) is positioned inside the second cover body (120), and the first cover body (110) may move relatively to the second cover body (120). The mixing device can realize liquid or solid, the mixing time in need of using function, also can be quantitative mixing. It is solved the problem of inaccuracy in a subsequent test which is caused by mixing, then storing and then using. In addition, it is simple in operation and convenient for use.

Description

A MIXING DEVICE

FIELD OF THE INVENTION

The present invention relates to a mixing device for mixing liquid or solid, in particular to a mixing device for mixing a liquid and a solid.

BACKGROUND OF THE INVENTION

The following background art is intended for helping a reader to understand the present invention, but shall be not regarded as the prior art.

The immune conjugation reaction principle is used for detecting whether or not an analyte is present in a sample, and this technique is widely used in various fields. Diseases and health status of human beings (early pregnancy, tumors, infectious diseases and drugs or the like) may be monitored by using this technique to detect analytes of various biospecimens (saliva, blood, urine, serum, sweat or the like) . The fundamental principle of this detection technology is based on the fact that immune molecules have a specific binding performance, for example, antibody/antigen, hapten/antibody, biotin/anti-biotin, etc. In addition, many detections like this may be completed on a solid medium, for example, a frequently-used transverse flow reagent strip, glass or plastic porous discs, an immunochromatography apparatus, etc. Generally, immune specific binding molecules may be further combined with some solid particles or chemical substances, so that a detection result may be obtained with the naked eye or by means of other apparatuses qualitatively, quantitatively or semi-quantitatively.

The detection reagent strips or apparatuses using these principles may be available from the prior art, for example, reagent strips or apparatuses containing reagent strips as described in the following patents: US 4857453； US 5073484； US 5119831； US 5185127； US 5275785； US 5416000； US 5504013； US 5602040； US 5622871； US 5654162； US 5656503； US 5686315； US 5766961； US 5770460； US 5916815； US 5976895； US 6248598； US 6140136； US 6187269； US 6187598； US 6228660； US 6235241； US 6306642； US 6352862； US 6372515； US 6379620； and US 6403383.

Generally, in the process of detecting these biospecimens, they need to be preprocessed so that analytes are released from the biospecimens. Therefore, preprocessing or buffering biospecimens becomes an indispensable key step to ensure an accurate or precise detection result.

In some detections, a sample under detection is preprocessed by adding a buffer solution. The buffer solution for buffering generally consists of one substance or two or more solid or liquid substances by mixing. In some detections, two substances (two different liquids, or one liquid and one solid substance) are mixed to form a buffer solution to buffer the sample. However, the two substances mixed in advance may react, or unfit for storing for longer time, or have a significantly decreased processing effect on the sample after storage for long time, or even cause inaccurate subsequent detections. Traditionally, sometimes, two different substances are separately stored generally. When it is necessary to process a sample, these substances are mixed and prepared by an operator on the spot, which not only increase cumbersome experimental steps, but also increases potential pollution risks. Therefore, it is indispensable to timely mix when a sample needs to be buffered.

SUMMARY OF THE INVENTION

The present invention provides a mixing device, through which a liquid or/and a solid originally separately stored may be mixed if needed, thereby implementing a function of timely mixing and processing, avoiding a cumbersome process of separately storing a sample and then allocating the sample by an experimenter on the spot before processing the sample traditionally, and reducing unnecessary pollution.

The mixing device (FIGS. 1-14) of the present invention includes a device body and a cover body covering and sealing the device body, where the cover body includes a first cover body and a second cover body； and the first cover body may move relatively to the second cover body. Specifically, the movement may be a rotational movement relatively to the second cover or an up-and-down movement relatively to the second cover. In some specific preferred embodiments, on the second cover body the first cover body includes a first position and a second position relatively to the second cover body； when the first cover body is in the first position, the first cover body can rotate instead of moving up and down relatively to the second cover body； when the first cover body and the second cover body are in the second position, the first cover body can move up and down relatively to the second cover body, so that the second cover body gets close to the first cover body. At this time, when the second cover body gets close to the first cover body or after the distance between both is close, it may become a locked second position (or a third position) . Closer distance between the second cover body and the first cover body may implement some advantageous technical effects. In some embodiments, the first cover body includes a first position, a second position and a third position on the second cover body. Preferably, when the first cover body is in the first position, the first cover body may rotate relatively to the second cover body； when the first cover body is in the second position, the first cover body may move up and down relatively to the second cover body； and when the first cover body is in the third position, the first cover body is fixed relatively to the second cover body. Preferably, when the first cover body is in the first position, the first cover body may rotate instead of moving up and down relatively to the second cover body； when the first cover body is in the second position, the first cover body may move up and down instead of rotating relatively to the second cover body； and when the first cover body is in the third position, the first cover body is fixed relatively to the second cover body or becomes a lock state.

In some embodiments, the first cover body and the second cover body are buckle structures. Preferably, when the first cover body is in the first position, the first cover body is not engaged with the second cover body, at this time, the first cover body may move up and down or rotate relatively to the second cover body in such a way: the first cover body rotates relatively to the second cover body and moves to a proper position, at this time, the first cover body moves up and down relatively to the second cover body to complete the up-and-down movement of the first cover body and the second cover body, so that the second cover body gets close to the first cover body, relatively, the first cover body gets close to the second cover body； when the first cover body is engaged and fixed to the second cover body, the second cover body and the first cover body are in a lock position.

In some embodiments, the bottom of the first cover body is provided with a piercing or puncturing structure. In other embodiments, the bottom of the second cover body is provided with a sealing structure. Preferably, when the first cover body is in the first position, the puncturing structure is not in contact with the sealing structure； and when the first cover body is moving from the first position to the second position, the puncturing structure is in contact with the sealing structure and punctures the sealing structure. Preferably, when the first cover body is in the third position, the puncturing structure at the bottom of the first cover body punctures the sealing structure at the bottom of the second cover body.

When the first cover body is in the first position, the first cover body is positioned inside the second cover body, and the first cover body is not engaged with the second cover body, at this time the puncturing structure at the bottom of the first cover body is inserted into an inner pot of the second cover body and is not in contact with the sealing structure, but is positioned above the bottom of the second cover body and is not in contact with the sealing structure at the bottom of the second cover body. Afterward, the first cover body is engaged to the second cover body or in the process or after the first cover body gets close to the second cover body, i.e., the first cover body reaches the second position or in the third position. In the engagement process, i.e., in the process when the first cover body arrives at the second position and the third position from the first position, the puncturing structure of the first cover body is in contact with the bottom of the second cover body and punctures the sealing structure at the bottom. In the present invention, generally a liquid or solid is placed inside the second cover body, another liquid or solid is placed inside the device body, after the first cover body punctures the second cover body, the liquid or solid inside the second cover body falls into the device body, thereby implementing the function of mixing two liquids or mixing a solid and a liquid.

In some specific embodiments, the second cover body includes a cavity body, the first cover body includes a rod body, a lower part of the rod body is positioned inside the cavity body of the second cover body； and the rod body may serve as the puncturing structure and move up and down inside the cavity body of the second cover body. In some other specific embodiments, at an upper part of the rod body of the first cover body there is provided with a convex rib； on the top of the second cover body there is provided with annular lug bosses forming a hollow cavity body in which the rod body passes across the cavity body and the convex rib on the rod body is blocked by the annular lug bosses formed. At this time, the first cover body may rotate but is not easy to move up and down relatively to the second cover body, only when the first cover body rotates to the recess or opening of the annular lug bosses can the lug boss on the rod body moves down along the opening, so that the distance between the first cover body and the second cover body is shortened. Preferably, little difference is between the lug boss on the rod body and the annular lug boss on the second cover body in terms of opening size. Once moving to a certain position where the first cover body is neither able to rotate left and right nor able to move up and down relatively to the second cover body, the first cover body and the second cover body are locked into an integrated structure. Preferably, on a side wall of the cavity body of the second cover body there is provided with a groove for receiving the convex rib. In a preferred embodiment, when the first cover body is in the first position, the convex rib on the rod body is positioned above a non-groove of the cavity body； when the first cover body is in the second position, the convex rib is positioned above the groove of the cavity body； and when the first cover body is in the third position, the convex rib is engaged and fixed inside the groove. Preferably, the convex rib is engaged and fixed inside the groove so that the first cover body and the second cover body are in a lock state.

The function of engagement of the first cover body and the second cover body is implemented by means of the convex rib on the rod body and the groove on the cavity body. In addition to such a structure, the relative movement of the first cover body and the second cover body may be implemented by other structures. When the first cover body is in the first position, the lower part of the rod body is inside the cavity body, but the bottom of the rod body is not in contact with the bottom of the cavity body. In addition, the upper part of the rod body and the convex rib at the upper part are outside the whole cavity body. Afterward, the first cover body is rotated so that the convex rib arrives at the groove on the cavity body, i.e., the second position. Now the bottom of the rod body is still not in contact with the bottom of the cavity body. Finally, the first cover body is pressed, so that the convex rib of the rod body moves downward along the groove of the cavity body until it arrives at the bottom of the groove, namely, the first cover body arrives at the third position of the second cover body. In the process when the convex rib is moving in the groove, namely in the process when the first cover body is moving from the second position to the third position, the rod body is moving downward inside the cavity body, and the bottom of the rod body gradually gets close to the bottom of the cavity body. In a more preferred embodiment, there are two convex ribs symmetrically distributed on the rod body； and there are two grooves symmetrically positioned on the cavity body of the second cover body. From the point of view of motion curve of the first cover body, at the beginning, the first cover body and the second cover body are in relatively fixed positions, by means of motion, the distance between the first cover body and the second cover body is shortened. By means of the physical distance shortening, it is implemented that solid or liquid is released after the puncturing structure punctures the sealing structure that seals the liquid or solid stored in the cover body. And the released liquid or solid subsequently is mixed with the solid or liquid inside the device body, thereby forming a new solution or mixture.

In some embodiments, the puncturing structure on the first cover body includes a lug boss positioned on the bottom of the rod body； and there may be one or more lug bosses. In a specific embodiment, the lug boss is an annular and keen-edged lug boss at the bottom of the rod body. In another preferred embodiment, the bottom of the rod body includes a concave pit for containing liquids or solids； and the annular lug boss is positioned at the edge of the concave pit. In other embodiments, the sealing structure on the second cover body is a puncturable sealing membrane positioned at the bottom of the cavity body.

In some embodiments, in order to ensure that liquid inside the mixing device does not leak out, although the first cover body may move relatively to the second cover body, the cover body may maintain the sealing of the device body, mainly the opening at one end of the device body is sealed. In some preferred embodiments, the cavity body on the device body may be sealed by the rod body on the first cover body. In a specific embodiment, the middle part of the rod body is provided with an annular lug boss for sealing the cavity body.

In some embodiments, the cover body is positioned at the bottom of the device body. As a part of the cavity body of the device body, the cover body needs to be sealed, particularly it still needs to keep tight after the second cover body is punctured. Therefore, it is sealed between the rod body of the first cover body and the cavity body of the second cover body. In this way, before or after mixing a liquid or a solid, the whole device can be kept in the device body, preventing the liquid or the solid from leaking out. In some embodiments, the first cover body is engaged and fixed to the second cover body, they can be detached from the device body, thereby exposing mixed solution in the device body and using for subsequent sample treatment. In other embodiments, the second cover body is provided with a screw thread mutually engaged to the device body. In some embodiments, in order to prevent a misoperation, the cavity body of the second cover body further has a fool-proof design, i.e., the cavity body of the second cover body further includes a concave hole； when the first cover body is in the first position, the convex rib is positioned inside the concave hole. In specific use, the first cover body is slightly lifted up until the convex rib on the rod body breaks away from the concave hole, then the first cover body is rotated to arrive at a designated location, and then the first cover body is moved relatively to the second cover body so that the distance between the first cover body and the second cover body is shortened, thereby releasing solid substances or releasing, by means of puncturing, the liquid or solid stored inside the second cover body.

In some preferred embodiments, the device body is a test-tube-shaped structure. First liquid is stored in the device body. One end of the test-tube is sealed by the cover body structure； the other end thereof is sealed by the cover body. After second liquid or solid in the cover body is mixed with liquid in the device body, the cover sealing the device body may be directly opened to implement use of liquid mixed. Of course, the device body may be combined with the cover body structure detachably. After the liquid or solid stored in the cover body is totally released, the cover body is detached from the device body, and the opening at one end of the device body is directly exposed for subsequent sample treatment, for example, a cotton swab on which sample is collected is inserted into the solution in the device body, or liquid is directly dropped onto a collected sample carrier such as a cotton swab, test paper, polyester fibre or other water-absorbing materials.

In another aspect (FIGS. 15-20) , the present invention provides a cover body device for storing and mixing a liquid or solid and a mixing device including the cover body. By means of the mixing cover body and the device, a liquid or/and a solid originally separately stored may be mixed if needed, thereby implementing a function of mixing at any time, i.e., the function of timely mixing and timely using.

In some preferred embodiments, different locations of the first cover body and the of the second cover body may be certainly implemented by means of a snap ring for liming location. And the snap ring may be detached from the first cover body and the second cover body. The following describes in detail how to implement such a function.

In another aspect, the cover body for storing and mixing a liquid or solid provided by the present invention includes an upper cover and a lower cover, where the upper cover may be movably connected with the lower cover； the lower cover includes a sealing cavity body； and the upper cover includes a puncturing structure and a sealing structure for puncturing and sealing the sealing cavity body of the lower cover. The sealing cavity body in the lower cover is used for storing liquid.

In some preferred embodiments, on the lower cover the upper cover is provided with a first position and a second position； when the upper cover is in the first position, the puncturing and sealing structure is positioned on an upper surface of the sealing cavity body and is not in contact with the upper surface； when the upper cover is in the process of moving from the first position to the second position, the puncturing and sealing structure is in contact with the upper surface of the sealing cavity body, punctures and seals the surface, and enters and squeezes the sealing cavity body, so that liquid in the sealing cavity body flows out； and when the upper cover is in the second position, the puncturing and sealing structure is totally inside the sealing cavity body.

By means of relative movement between the upper cover and the lower cover, liquid sealed inside the lower cover can be transferred into the upper cover, in which, a solid or liquid is prestored. In this way, it is implemented mixing two liquids or a liquid and a solid. In other words, in the process of relative movement between the upper cover and the lower cover, liquid is transferred from the sealing cavity body of the lower cover to the upper cover, which becomes a key point for implementing mixing of a liquid or solid. In the present invention, this function of liquid transferring is implemented by puncturing and sealing the sealing cavity body by means of the puncturing and sealing structure on the upper cover and moving and squeezing the sealing cavity body to force liquid inside the sealing cavity body to flow out. Specifically speaking, first of all, the puncturing and sealing structure punctures the surface of the sealing cavity body so that the sealing cavity body is communicated with the upper cover. Simultaneously, the puncturing and sealing structure seals the punctured surface of the sealing cavity body so that the sealing cavity body is sealed again in addition to the channel communicated with the upper cover. Finally, the puncturing and sealing structure continues moving toward the bottom of the cavity body, squeezing the sealing cavity body to force the liquid to flow out into the upper cover along the channel. Namely, when the upper cover is in the first position, solids or liquids on the upper cover and the lower cover are respectively stored, thereby implementing the storage function of the cover body. At the moment, the puncturing and sealing structure is positioned on the upper surface of the sealing cavity body and is not in contact with the upper surface； when the upper cover is in the second position, the cover body implements the mixing function, at the moment, the puncturing and sealing structure is totally inside the sealing cavity body, and all or a large proportion of liquid inside the cavity body is squeezed to flow into the upper cover and mix with the liquid or solid originally stored in the upper cover. To the upper cover, the process of moving from the first position to the second position is the process of mixing the liquid or solid. At the moment, the puncturing and sealing structure is in contact with the upper surface of the sealing cavity body, punctures and seals the surface, and gradually enters and squeezes the sealing cavity body, so that liquid in the sealing cavity body flows into the upper cover and gradually mixes with the liquid or solid inside the upper cover.

In some specific embodiments, the puncturing and sealing structure includes a conical lug boss. The lug boss is conical, while it is ensured that the keen-edged lug boss at the top can puncture the surface of the sealing cavity body, the volume of liquid entering into the sealing cavity body is gradually increased, i.e., the volume of the sealing cavity body is gradually reduced, so that the process of compression or outflow of the liquid inside the sealing cavity body is progressive, thereby avoiding phenomena of slow outflow or sudden blowout.

Preferably, the puncturing and sealing structure further includes a cylinder connected to an end of the conical lug boss.

More preferably, the diameter of the cylinder is equal to the inside diameter of the sealing cavity body.

In other embodiments, the puncturing and sealing structure further includes a bottleneck cylinder connected between the lug boss and the cylinder. In this way, the lug boss is smoothly connected to the cylinder, also liquid does not jet out due to sudden pressure increase resulted from reduction of space as the puncturing device squeezes the cavity body to force the liquid to flow out.

After the cylinder enters into the sealing cavity body, a seal is formed between the cylinder and the side wall of the sealing cavity body, so that the puncturing and sealing structure becomes a piston. In the process of moving downward inside the sealing cavity body, the cylinder is appressed to the inside wall of the sealing cavity body and moves, and the space of the remaining sealing cavity body is gradually reduced in a sealing stat, so that enough pressure is maintained inside the cavity body, and liquid therein flows out of the cavity body until the puncturing and sealing structure is filled in the sealing cavity body.

In order to ensure liquid inside the sealing cavity body is sufficiently transferred out, in a specific embodiment, the length of the conical lug boss is smaller than one half of that of the sealing cavity body.

In some embodiments, the puncturing and sealing structure further includes a channel through which liquid inside the sealing cavity body flows into the upper cover.

More specifically, the opening of the channel is positioned on the conical lug boss. In this way, the channel may be communicated with the liquid inside the cavity body in the first time.

In other specific embodiments, the upper surface of the sealing cavity body includes a puncturable membrane or rubber.

In some specific embodiments, the cover body includes a clasp that prevents the upper cover and the lower cover from moving； and the clasp may be detached from the cover body.

When the clasp is fixed to the cover body, the upper cover cannot move relatively to the lower cover, at the moment, the upper cover is in the first position of the lower cover. After the clasp (also referred to as the snap ring) is removed from the cover body, the upper cover can move relatively to the lower cover, specifically speaking, the upper cover can move, along the lower cover, towards the bottom of the lower cover, and finally arrives at the second position. In some embodiments, between the upper cover and the lower cover is a threaded connection, i.e., movement of the upper cover and the lower cover is implemented by means of thread engagement.

In another aspect, the present invention further provides a device for storing and mixing liquid or solid, including a device body and a cover body of the present invention, and the device body is connected with the upper cover of the cover body and is communicated with liquid.

In a more specific embodiment, the channel of the puncturing and sealing structure is communicated with liquid inside the device body.

The device body is connected and communicated with the upper cover, so the liquid entering into the upper cover can also enter into the device body, in which, the entered liquid is mixed with a liquid or solid originally stored inside the device body. More specifically, liquid may enter into the device body through the channel, of the puncturing and sealing structure, which is directly communicated with the device body.

In another aspect (FIGS. 21-26) , the present invention provides a device for storing and timely mixing a liquid or solid and a method for mixing at least two substances by using the device. By means of the mixing device and the method, a liquid or/and a solid originally separately stored may be mixed if needed, thereby implementing a function of mixing at any time, i.e., the function of timely mixing and timely using.

The device for mixing at least two substances of the present invention includes a pedestal and a container connected to the pedestal, where the pedestal includes a piston cavity body and a piston positioned inside the piston cavity body； the bottom of the container is communicated with the piston cavity body on the pedestal and is sealed by the piston； and the piston may move inside the piston cavity body.

In a preferred embodiment, the bottom of the container includes a through hole, and the container is communicated with piston cavity body by means of the through hole.

In another embodiment, the external surface of the piston includes at least two sealing regions.

Specifically, the through hole at the bottom of the container is positioned within the sealing regions and is sealed by the sealing regions.

In some other preferred embodiments, each sealing region includes a groove which is communicated with the through hole at the bottom of the container. In the groove of the sealing region, substances such as liquid or solid or the like that need mixing may be prestored.

In some embodiments, the piston includes at least a first position and a second position in the piston cavity body.

In a specific embodiment, when the piston is in the first position, the first sealing region of the piston is positioned at the bottom of the container and seals the through hole at the bottom of the container. More specifically, the through hole at the bottom of the container is communicated with the first groove inside the first sealing region and is sealed by the groove. The solid or liquid inside the groove is communicated and mixed with the substance inside the container.

In another specific embodiment, when the piston is in the second position, a second sealing region of the piston is positioned at the bottom of the container and seals the through hole at the bottom of the container. Similarly, at the moment, the through hole at the bottom of the container is communicated with the second groove inside the second sealing region and is sealed by the groove. The solid or liquid inside the second groove is communicated and mixed with the substance inside the container.

In a preferred embodiment, the surface of the piston includes multiple sealing rings and sealing lines, and the piston is divided, by the sealing rings and the sealing lines, into at least two sealing regions.

In some other embodiments, the piston includes a push rod positioned inside the piston.

In some embodiments, the push rod has an initial position and an end position inside the piston.

In a preferred embodiment, when the push rod is in the initial position, the piston cannot relatively move inside the piston cavity body； when the push rod is in the end position, driven by the push rod, the piston can move back and forth inside the piston cavity body.

In another preferred embodiment, when the pull rod is in the initial position, the piston has a smaller size and the pull rod can move relatively to the piston； when the pull rod is in the end position, the piston has a larger size and the pull rod cannot move relatively to the piston.

In a specific embodiment, the push rod includes a rod shank and a buckle structure connected to the rod shank； and the piston includes a corresponding mechanism matching up with the buckle structure.

More specifically, when the push rod is in the initial position, the buckle structure of the push rod and the corresponding mechanism of the piston are in an unfastened state, so that they can move relatively； when the push rod is in the end position, the buckle structure of the push rod and the corresponding mechanism of the piston are in a fastened state, so that they cannot move relatively.

In a specific embodiment, the buckle structure includes two elastic arms and a lug positioned at an outer side of a free end of the elastic arm.

In another specific embodiment, the corresponding mechanism of the piston includes a channel for receiving the elastic arm and a lock-hole corresponding to the lug.

When the push rod is in the initial position, the two elastic arms of the push rod and the lug positioned at the outer side of the free end of the elastic arm are received in the channel of the corresponding mechanism inside the piston； when the push rod is in the end position, the lug at the free end of the elastic arm is buckled in the lockhole of the corresponding mechanism inside the piston. Namely, when the push rod is pulled out of the piston, the elastic arm moves inside the channel of the piston until the lug on the elastic arm arrives at the lockhole on the piston and is engaged by the lockhole, thereby arriving at the end position. At the moment, the push rod and the piston are engaged and fixed.

In some other embodiments, the piston further includes a lock slot and a stop pin inside the lock slot. When the piston is in the first position, the stop pin is positioned in the lock slot, and the piston cannot move inside the cavity body. When the piston needs to move inside the cavity body, the stop pin is removed, and the piston is pushed inside the piston cavity body so that the piston arrives at the second position, thereby finishing movement of the piston inside the cavity body.

The present invention further includes a method for mixing at least two substances, including: there is provided with a mixing device, which includes a pedestal and a container connected to the pedestal, where the pedestal includes a piston cavity body and a piston received in the piston cavity body, inside the piston cavity the piston at least has a first position and a second position, at the joint where the container is connected to the pedestal there is provided with a through hole communicated with the piston cavity body, on the surface of the external wall of the piston there is provided with at least a first region and a second region. And the method includes the following steps:

a) a first substance is stored in the second region on the surface of the external wall of the piston；

b) the piston is disposed in the first position inside the piston cavity, the through hole at the joint where the container is connected to the pedestal is connected with the first region on the surface of the external wall of the piston, and is enclosed and sealed by the first region；

c) a second substance is stored in the container；

d) the piston moves, inside the piston cavity, from the first position to the second position, the through hole at the joint where the container is connected to the pedestal is connected with the second region on the surface of the external wall of the piston, and is enclosed and sealed by the second region； and

e) the first substance is mixed with the second substance.

Preferably, the first region and the second region include a groove for receiving a substance, and the groove is communicated with the through hole at the bottom of the container and seals the through hole.

Preferably, the piston further includes push rod that has an initial position and an end position inside the piston. When the push rod is in the initial position, the piston has a smaller size and the push rod can move relatively to the piston； when the push rod is in the end position, the piston has a larger size and the push rod cannot move relatively to the piston.

Preferably, the push rod includes a shank part and a buckle structure extending from the shank part. The piston includes a corresponding mechanism for receiving the buckle structure of the push rod. When the push rod is in the initial position, the buckle structure of the push rod and the corresponding mechanism of the piston are in an unfastened state, so that they can move relatively； when the push rod is in the end position, the buckle structure of the push rod and the corresponding mechanism of the piston are in a fastened state, so that they cannot move relatively.

Preferably, the buckle structure of the push rod includes two elastic arms, and a lug is disposed at an outer side of a free end of either of the elastic arms. The corresponding mechanism inside the piston includes a channel for receiving the elastic arms and a lockhole corresponding to the lug. When the push rod is in the initial position, the elastic arms of the push rod and the lug are compressed and received in the channel of the corresponding mechanism inside the piston. When the push rod is in the end position, the lug at the free end of the elastic arm of the push rod is buckled in the lockhole of the corresponding mechanism inside the piston.

More preferably, between the Step c) and the Step d) a Step f) is further included: the push rod is moved from the initial position to the end position.

In in another preferred embodiment, between the Step a) and the Step b) a Step g) is further included: a third substance is placed in the first region on the external surface of the piston； between the Step c) and the Step f) a Step h) is further included: the third substance is mixed with the second substance.

More specifically, the third substance is place in the first groove of the first region.

Beneficial Effects

The mixing device of the present invention can implement a function of mixing liquid or solid, particularly mixing a liquid and a solid at any time when they are needed for use, can also implement a quantitative mixing. It is solved the problem of inaccuracy in a subsequent test which is caused by mixing, then storing and then using. In addition, it is simple in operation and convenient for use.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic diagram of a mixing device of the present invention；

FIG. 2 is a sectional drawing of the device in FIG. 1；

FIG. 3 is a schematic diagram of a first cover body of the device in FIG. 1 being positioned in a first position of a second cover body；

FIG. 4 is a schematic diagram of the first cover body of the device in FIG. 1 being positioned in a second position of the second cover body；

FIG. 5 is a schematic diagram of the first cover body of the device in FIG. 1 being positioned in a third position of the second cover body；

FIG. 6 is a sectional drawing of the first cover body of the device in FIG. 1 being positioned in the first position of the second cover body；

FIG. 7 is a sectional drawing of the first cover body of the device in FIG. 1 being positioned in the third position of the second cover body；

FIG. 8 is a schematic diagram of another mixing device of the present invention；

FIG. 9 is a sectional drawing of the device in FIG. 8；

FIG. 10 is a schematic diagram of a first cover body of the device in FIG. 8 being positioned in a first position of a second cover body；

FIG. 11 is a schematic diagram of the first cover body of the device in FIG. 8 being positioned in a second position of the second cover body；

FIG. 12 is a schematic diagram of the first cover body of the device in FIG. 8 being positioned in a third position of the second cover body；

FIG. 13 is a sectional drawing of the first cover body of the device in FIG. 8 being positioned in the first position of the second cover body；

FIG. 14 is a sectional drawing of the first cover body of the device in FIG. 8 being positioned in the third position of the second cover body；

FIG. 15 is a schematic diagram of the mixing device according to a specific embodiment of the present invention；

FIG. 16 is a schematic diagram of a storing and mixing device according to a specific embodiment of the present invention；

FIG. 17 is a schematic diagram of a clasp being detached from the cover body；

FIG. 18 is a sectional drawing of the upper cover of the device in FIG. 16 being in the first position of the lower cover；

FIG. 19 is a sectional drawing of the clasp being removed from the device in FIG. 16；

FIG. 20 is a sectional drawing of relative motion of the upper cover and the lower cover of the device in FIG. 16；

FIG. 21 is a schematic breakdown drawing of the device according to a specific embodiment of the present invention；

FIG. 22 is a principal view of the device as shown in FIG. 21 of the present invention；

FIG. 23 is a sectional drawing when the piston of the device as shown in FIG. 21 is in the first position and the push rod is in the initial position；

FIG. 24 is a partial sectional drawing of FIG. 23；

FIG. 25 is a partial sectional drawing when the piston of the device is in the first position and the push rod is in the end position； and

FIG. 26 is a partial sectional drawing of the device when the piston is in the second position and the push rod is in the end position.

Reference Numbers in the Attached Drawings:

Mixing device 100, 800； 1800； 80； first cover body 110, 810； second cover body 120, 820； device body 130, 830； cover top of the first cover body 111, 811； upper part of the rod body of the first cover body 113, 813； lower part of the rod body of the first cover body 112, 812； lug boss at the bottom of the rod body 115, 815； concave pit at the bottom of the rod body 816； upper sealing ring of the rod body 116； convex rib at the upper part of the rod body 114, 814； lower part of the cavity part of the second cover body 122, 822； upper part of the cavity part 123, 823； concave groove at the upper part of the cavity part 124, 824； concave hole at the upper part of the cavity part 125, 825； sealing membrane at the bottom of the cavity body 121, 821； cap of the second cover body 126； internal thread of the cap 127； thread at the opening of the device body 131； solid inside the second cover body 140, 840； liquid inside the device body 150, 850； another upper cover of the device body 860； cover body 1100； lower cover 1110； upper cover 1120； buckle 1130； device body 1120； upper cover of device body1210； liquid inside the lower cover 1130； sealing cavity body of the lower cover 1112； sealing surface of the sealing cavity body 1113； internal thread of the upper cover 1121； puncturing and sealing structure 1122, lug boss 1221； cylinder 1222； bottleneck cylinder 1223； channel 1224； channel opening 1225； solid 1400； external thread of the lower cover 1111； container 1； through hole at the bottom of the container 101； pedestal 200； piston cavity body of the pedestal 201； piston 300； external surface of the piston 310； push rod 320； handle of the push rod 321； elastic arm of the push rod 322； upper lug boss of the elastic arm 323； first sealing region 311； second sealing region 312； first concave groove inside the first sealing region 313； second concave groove inside the second sealing region 314； channel of the piston 315； piston lockhole 316； sealing ring on the piston 318； sealing line on the piston 317； cover on the container 400； liquid 600； and solid 500.

DETAILED DESCRIPTION OF THE EMBODIMENTS

The following further describes structures involved in the present invention or these adopted technical terms. In the following detailed description, reference words appended to figures are a part of the paper, which is described by illustrating any practicable specific scheme of the present invention. It is not excluded that other specific schemes may be implemented in the present invention and that the structure of the present invention may be changed without departing from the scope of application of the present invention.

According to one aspect, in the present invention, a substance is respectively and separately stored in the device body and the cover body of the mixing device. When it is needed mixing, the substance in the cover body can enter into the device body to mix. In this way, it is implemented the function of separately storing and mixing for using at any time. Specifically speaking, when it is needed to mix two substances, the substance originally stored closely on the cover body can be released. In other words, the cover body needs such a structure that is sealed when it needs to implement storing and open when it needs to implement mixing, so that the cover body is communicated with the device body when it needs to implement mixing. In some embodiments, the cover body may be divided into two parts movably connected. When the cover body needs to be sealed, the two parts of the cover body is in a connection status. When the cover body needs to be communicated with the device body, the two parts of the cover body is in another connection status (see FIGs. 1-14 and the following detailed description) .

In some embodiments, as shown in FIG. 1 and FIG. 8, the cover body is divided into a first cover body and a second cover body, where the first cover body is positioned inside the second cover body, the first cover body may move relatively to the second cover body, to implement the requirements of the cover body for sealing and opening. More specifically, on the second cover body the first cover body has three positions: a first position, a second position and a third position. By means of position change of the first cover body on the three positions, it is implemented the function that the sealing state of the cover body is broken and the whole cover body is communicated with the device body. specifically, when the first cover body is in the first position, the first cover body may rotate relatively to the second cover body； when the first cover body is in the second position, the first cover body may move up and down relatively to the second cover body； and when the first cover body is in the third position, the first cover body is fixed relatively to the second cover body. In some embodiments, the first cover body and the second cover body are buckle structures, which may implement the foregoing function. Specifically, when the first cover body is in the first position and the second position, the first cover body is not engaged with the second cover body； and when the first cover body is in the third position, the first cover body is engaged and fixed to the second cover body.

In order to implement the function that the cover body can be communicated with the device body at any time and that the solid or liquid on the cover body is mixed with the solid or liquid inside the device body, the first cover body is provided with a puncturing structure, and at the bottom of the second cover body there is provided with a sealing structure. When the first cover body is in the first position and the second position, the puncturing structure is not in contact with the sealing structure； and when the first cover body is in the third position, the puncturing structure on the first cover body punctures the sealing structure at the bottom of the second cover body. Namely, in the process when the first cover body is moving from the second position to the third position, the puncturing structure on the first cover body gradually gets close to and contacts the sealing structure at the bottom of the second cover body, finally the sealing structure is punctured so that the liquid or solid inside the sealing structure breaks away from the second cover body and enters into the device body and mix with the liquid or solid therein, thereby implementing mixing of the two substances.

As shown in FIG. 1, the mixing device 100 in an embodiment of the present invention includes a first cover body 110, a second cover body 120 and a device body 130. By means of the cap 126, the second cover body covers and is connected hermetically with the device body 130. Specifically, the internal thread 127 of the cap 126 is mutually engaged to the external thread 131 at the opening of the device body 130 so that the second cover body 120 is fixed to the device body 130. The device body 130 contains a buffer solution 150, as shown in FIG. 2. The first cover body 110 consists of a cover top 111 and a rod body connected to the cover top, where the rod body is divided into an upper part 113 and a lower part 112. The second cover body 120 consists of a cavity body and a cap 126 connected to an outside of the cavity body, for coupling to the device body 130. The cavity body includes an upper cavity body 123 and a lower cavity body 122. When the first cover body 110 is connected to the second cover body 120, the lower part 112 of the rod body is positioned inside the lower cavity body 122, as shown in FIG. 6. The upper part 113 of the rod body is provided with two convex ribs 114 symmetrically distributed on the rod body 113. Correspondingly, the upper cavity body 123 is provided with two symmetrical grooves 124 for receiving the convex ribs 114. In addition, the bottom of the rod body further includes a lug boss 115 for puncturing the bottom of the cavity body, and an annular lug boss-sealing ring 116, which is positioned in the middle part of the rod body and used for sealing the cavity body. The bottom of the cavity body of the second cover body 120 has a sealing membrane 121, on which there is provided with solid substance 140. In addition, the upper cavity body 123 is further provided with a concave hole 125, used for preventing the rod body 112 from moving inside the cavity body 122 due to a mis-operation.

The following will make a detailed description with reference to the structure of the device 100 and specific operating steps of the device 100.

As shown in FIG. 3 and FIG. 6, when the first cover body 110 is in the first position, i.e., the initial state, the lower part 112 of the rod body of the first cover body is positioned inside the cavity body 122 of the second cover body, where the sealing ring 116 on the rod body seals the cavity body 122. At the moment, the upper part 113 of the rod body is positioned outside the cavity body 123, the convex rib 114 is also positioned above a non-groove outside the cavity body 123. The lug boss 115 at the bottom of the rod body 112 is positioned inside lower cavity body 122, a section of distance from the sealing membrane 121 at the bottom. By means of the internal thread 127, the cap 126 of the second cover body is engaged to the external thread 131 at the cavity opening of the device body 130, and the first cover body 110 and the second cover body 120 seal the device body 130.

Then, the first cover body 110 is rotated so that the first cover body 110 moves relatively to the second cover body 120 and the device body 130 and arrives at the second position, namely, the convex rib 114 on the rod body arrives at the groove 124 on the cavity body, as shown in Fig. 4. At the moment, the distance between the lug boss 115 at the bottom of the rod body and the sealing membrane 121 at the bottom of the cavity body is unchanged, and they are not in contact with each other.

Then the first cover body 110 is pressed down or pushed, so that the first cover body 110 moves from the second position to the third position, as shown in FIG. 5 and FIG. 7. In this process, the convex rib 114 on the rod body moves deep in the groove 124 of the cavity body. When the convex rib 114 arrives at the bottom of the groove 124, the convex rib 114 is obstructed, and thus cannot move further. The convex rib 114 is engaged and fixed by the groove 124, i.e., the first cover body 110 is engaged and fixed by the second cover body 120. At the same time, the rod body 112 moves, in the cavity body 122, towards the bottom of the cavity body, in the moving process, the lug boss 115 at the bottom of the rod body gradually gets close to the sealing membrane 121 at the bottom of the cavity body, contacts and punctures the sealing membrane 121. Once the sealing membrane 121 is punctured, the solid substance 140 thereon breaks away from the bottom of the cavity body together with the sealing membrane 121, and falls into the device body 130 connected with the second cover body, and mixes with liquid 150 inside the device body.

In some embodiments, in order that the sealing membrane 121 can be effectively punctured, at the bottom of the rod body there may be provided with multiple lug bosses 115 uniformly distributed at the bottom of the rod body 112. In another embodiment, the lug boss 115 is an annular and keen-edged lug boss at the bottom of the rod body.

In some other embodiments, after the solid is mixed with the liquid, the first cover body 110 is engaged to the second cover body 120, and they can be detached from the device body 130. After detachment, the sample that needs to be detected may be added, through the cavity opening, into the device body 130.

FIGS. 8-14 are schematic diagrams of a mixing device 800 according to another embodiment of the present invention. Specific description is as below: different from the previous embodiment, after a first cover body 810 is connected to a second cover body 820, they are positioned at the lower part of a device body 830, forming the bottom of the device body 830. The device body 830 is provided with liquid solution 850, and another upper cover 860 covers the upper cavity opening of the device body 830.

As shown in FIG. 8 and FIG. 9, the first cover body 810 includes a cover top (cover bottom) 811 and a rod body, where a convex rib 814 is positioned at an upper part 813 of the rod body, the bottom of the rod body is provided with a concave pit 816 for receiving liquid or solid, and the concave pit is internally provided with solid substance 840. At the edge of the concave pit 816 there is provided with an annular and keen-edged lug boss 815 for puncturing a sealing membrane. The second cover body 820 includes an upper part 823 and a lower part 822 of the cavity body, where the lower part 822 of the cavity body of the second cover body is positioned inside the lower cavity body of the device body 130, and they are hermetically fixed. At the bottom of the cavity body 822 of the second cover body there is provided with a sealing membrane 821 that seals one end of the cavity body. Similarly, the upper part of the cavity body is provided with a concave hole 825 preventing the convex rib from sliding.

When the first cover body is in the first position, as shown in FIG. 10 and FIG. 13, the first cover body 810 is connected with the second cover body 820, the bottom of the device body 830 is sealed, and the device body is filled with the liquid 850. At the moment, the second cover body 820 is separated, by the sealing membrane 821 at the bottom of the cavity body, from the device body 830, being in a disconnection state. The lower rod body 812 of the first cover body is positioned inside the cavity body 822 of the second cover body, the rod body 812 seals the cavity body 822, and the rod body 812 may be provided with a sealing ring (not shown in FIGs. ) . At the moment, the annular lug boss 815 at the bottom of the rod body is not in contact with the sealing membrane 821 at the bottom of the cavity body, a certain distance being kept between both. The convex rib on the rod body is positioned at a non-groove above the cavity body of the second cover body, and more specifically, positioned in the foolproof concave hole 825.

Then, the first cover body 810 is slightly lifted up until the convex rib 814 breaks away from the concave hole 825, then the first cover body 810 is rotated to arrive at the second position, as shown in FIG. 11, the convex rib 114 arrives above the groove 824. In this process, the rod body 812 rotates inside the cavity body 822, and the distance between the keen-edged lug boss 815 at the bottom of the rod body and the sealing membrane 821 at the bottom of the cavity body is unchanged.

Then the first cover body 810 is pressed again, so that the convex rib 814 moves inside the groove 824, and finally arrives at the bottom of the groove 824 and is fixed, i.e., the first cover body 810 arrives at the third position of the second cover body 820, as shown in FIG. 12 and FIG. 14. In this process, the

rod body

812 and 813 moves up and down inside the

cavity body

822 and 823. In the moving process, the annular lug boss 815 at the bottom of the rod body gradually gets close to the bottom of the cavity body and finally contacts with and punctures the sealing membrane 821. After the sealing membrane 821 is punctured, the cavity body 822 is communicated with the device body 830, and the bottom of the rod body 812 enters into the device body 830, and contacts with the liquid 850 inside the device body 830. At the moment, the solid substance 840 in the concave pit 816 is mixed with and dissolved in the liquid 850 inside the device body, forming a mixture. In some embodiments, the

device body

130 and 830 may be made from compressible material, so that mixing may be more intensive by squeezing the

device body

130 and 830.

In another specific embodiment, the present invention further provides a mixing device, as shown in FIGs. 15-20. The device includes a cover body 1100 which is divided into two parts: an upper cover 1120 and a lower cover 1110, which may be movably connected with each other. In an initial state, the upper cover 1120 is fixedly connected with the lower cover 1110. In this way, liquid or solid may be separately stored in the cover body 1100 without changing their states. After the upper cover 1120 moves relatively to the lower cover 1110, the solid or liquid separately stored in the upper cover 1120 and the lower cover 1110 may be mixed. As shown in FIG. 15, in a specific embodiment, the upper cover 1120 is connected to the lower cover 1110 by means of mutual engagement of internal and external threads, and the upper cover 1120 is fixed to the lower cover 1110 by means of a buckle 1130 so that they cannot move. The buckle 1130 is positioned on the lower cover 1110. There is certain distance between the bottom of the upper cover 1120 and the bottom of the lower cover 1110. When it is required that the upper cover 1120 moves relatively to the lower cover 1110, the buckle 1130 is removed from the cover body 1100, as shown in FIG. 17, the upper cover 1120 is rotated downward, in this way, the upper cover 1120 can move on the lower cover 1110.

As shown in the sectional drawing of FIG. 18, the lower cover 1110 includes a sealing cavity body 1112 which is internally filled with liquid solution 1300, for example, buffer solution. The bottom and the side wall of the sealing cavity body 1112 is an integrated structure, which is positioned inside the cavity of the lower cover 1110. The upper surface 1113 of the sealing cavity body is sealed by means of the sealing membrane or sealing rubber. The sealing membrane or sealing rubber can be punctured by a keen-edged object, so that it is convenient for the liquid inside the cavity body to be communicated with the outside.

The upper cover 1120 includes a puncturing structure 1122 that can puncture and then seal the upper surface of the cavity body, similarly as shown in FIG. 18 or 19.Specifically speaking, the puncturing and sealing structure 1122 consists of three parts connected together: a conical lug boss 1221, a bottleneck cylinder 1223 and a cylinder 1222. The conical lug boss 1221 is mainly used for puncturing the sealing membrane or sealing rubber of the surface 113 of the sealing cavity body and then sealing the surface 113. One end (with a smaller diameter) of the bottleneck cylinder 1223 is connected to the tail end of the lug boss 1221, the other end is connected to the cylinder 1222, which is mainly used for sealing the surface 1113 of the sealing cavity body, and then sealing the side wall of the cavity body 1112, so that space of the sealing cavity body 1112 is sealed and reduced, and liquid 1300 inside the cavity body 1112 is squeezed to flow out. The diameter of the cylinder 1222 is the same as the inside diameter of the sealing cavity body 1112, so that it is ensured that a sealing is formed between the external side wall of the cylinder and the internal side wall of the cavity body.

In order that the liquid 1300 inside the cavity body 1112 can be sufficiently transferred into the upper cover 1120, the lug boss 1221 needs to occupy less space so that the cylinder 1222 connected to the lug boss further enters into the cavity body 1112. Therefore, the shorter the height of the lug boss 1221 is, the better it is. In some specific embodiments, the length of the conical lug boss 1221 is smaller than one half of that (height) of the sealing cavity body 1112.

The puncturing structure 1122 of the upper cover 1120 further includes a channel 1224 communicated with the sealing cavity body 1112. Through the channel 1224, the liquid 1300 inside the cavity body 112 is transferred into the upper cover 1120, and then arrives at other structures connected to the upper cover 1120. Generally, the channel opening 1225 is positioned on the lug boss 1221, to ensure that the liquid 1300 can be timely transferred into the channel 1224. In a specific embodiment, the opening 1225 is positioned at the top of the lug boss 1221, as shown in FIG. 6.

As shown in FIG. 16, the present invention further includes a mixing device 1800, which includes a cover body 1100 and a device body 1200, where the upper cover 1120 of the cover body is connected with the device body 1200.

As shown in FIG. 20, the channel 1224 is a hollow slender pipe, which is connected with the device body 1200, so that the liquid 1300 inside the lower cover 1110 can be directly transferred, through the channel 1224, into the device body 1200. Additionally, the slender channel 1224 allows sufficient liquid to be transferred into the device body 1200 instead of being wasted inside the channel 1224.

The following describes in detail the cover body 1100 and the device 1800 of the present invention with reference to specific operating procedures.

During production, the required liquid 1300 is stored by the mixing device 1800 into the cavity body 1112 of the lower cover, then the surface 1113 of the cavity body is sealed by the sealing membrane, so that the liquid 1300 is sealed and stored in the cavity body 1112 of the lower cover.

The upper cover 1120 and the lower cover 1110 are fixed to form the cover body 11100 by means of engagement of the internal thread 1121 of the upper cover and the external thread 1111 of the lower cover. In addition, the middle-lower part of the lower cover 1110 is further fixedly provided with a clasp 1130, by means of the clasp 1130, the upper cover 1120 and the lower cover 1110 are limited and fixed in position.

The top of the upper cover 1120 is connected with the device body 1200 which is internally provided with solid 1400 separately stored, and the device body 1200 includes the upper cover 1120 covering and sealing the device body.

The upper cover 1120 is internally provided with a puncturing and sealing structure 1122, and they may become an integrated structure by fixedly connecting the tail end of the cylinder 1222 on the structure 1122 with the upper cover 1120. The other end of the cylinder 1222 is connected to the bottleneck cylinder 1223 which is connected to the conical lug boss 1221. The length of the whole puncturing and sealing structure 1122 is equal to the height (length) of the sealing cavity body 1112.

When the upper cover 1120 and the lower cover 1110 are fixed by the clasp 1130, i.e., when the upper cover 1120 is in the first position of the lower cover 1110, as shown in FIG. 4, the puncturing structure 1122 is positioned above the upper surface 1113 of the sealing cavity body, and there is a certain distance between the lug boss 1221 and the upper surface 1113.

When it is needed to mix a solid and a liquid, the clasp 1130 engaged and fixed to the lower cover is removed, as shown in FIG. 17 and FIG. 19, then the upper cover 1120 is rotated so that on the lower cover 1110 the upper cover 1120 moves towards the bottom of the lower cover 1110. Meanwhile, the puncturing structure 122 inside the upper cover also gets close to the sealing cavity body 1112, and then the lug boss 1221 contacts with the sealing membrane on the upper surface 1113 of the sealing cavity body. As the upper cover 1120 continues rotating, the lug boss 1221 squeezes and punctures the sealing membrane 1113, and enters into the cavity body 1112. At the moment, the lug boss 1221 works closely with the sealing membrane 1113, and the sealing cavity body 1112 is still sealed. The liquid 1300 inside the cavity body is squeezed because space is occupied by the lug boss 1221, and enters, through the through hole opening 1225, into the channel 1224 on the lug boss. As the lug boss 1221, the bottleneck cylinder 1223 connected with the lug boss, and the cylinder 1222 enter into the cavity body 1112, the puncturing structure 1122 comprehensively seals and occupies space of the cavity body 1112, so that more and more liquid 1300 inside the cavity body enters into the channel 1224. Through the slender channel 1224, the liquid 1300 enters into the device body 1200 communicated with the channel, contacts with the solid 1400 and mixes and dissolves the solid, as shown in FIG. 20. Finally, when the upper cover 1120 arrives at the second position (not shown in FIGs. ) , it arrives at the bottom of the lower cover 1110. The upper cover 1120 stops rotating, and is fixed relatively to the position of the lower cover 1110. At the moment, the puncturing structure 1122 totally enters into the sealing cavity body 1112, and occupies most of the volume of the cavity body 1112, so that the liquid 1300 inside the cavity body is sufficiently squeezed and discharged. In this way, the device body 1200 has sufficient quantity of mixed liquid, thereby ensuring a successful subsequent testing.

In another aspect, the present invention provides another mixing device, as shown in FIGs. 21-26, the device 80 of the present invention includes a pedestal 200, and a container 1 connected to the pedestal. The container 1 (the container may be a test-tube, a tube, or any a vessel for receiving liquid) may be fixedly or detachably connected with the pedestal 200. The bottom of the container is communicated with the pedestal or the piston cavity body inside the pedestal, and liquid or fluid inside the container may flow into the piston cavity body. In a specific embodiment, the pedestal 200 and the container 1 are an integrated structure. The top of the container 1 further includes a cover 400 for covering or sealing the container, to prevent substance stored in the container 1 from volatilizing or leaking out, as shown in FIG. 22.The container and the pedestal may be shaped like an inverse “T” , the vertical axis of the container is perpendicular to the horizontal axis of the pedestal (FIG. 22) . Of course, the container may also be connected with the pedestal at an arbitrary angle, for example, an L shape, etc.

The pedestal 200 includes a piston cavity body 201 and a piston 300 (FIG. 22) contained inside the piston cavity body. And, the piston 300 may move relatively to the piston cavity body 201. As shown in FIG. 23, space of the piston cavity body 201 is larger than the volume of the piston 300, which allows that the piston 300 may move, inside the piston cavity body 201, forward a certain distance along the horizontal axis of the piston cavity body. As shown in FIG. 21, the piston cavity body 201 is positioned on the pedestal 200, and the cavity body 201 is connected and communicated with the bottom 101 of the container 1. The piston 300 is positioned inside the piston cavity body 201 of the pedestal, and is communicated with the bottom 101 of the container. The may be in contact with or not in contact with the liquid inside the container in different states or positions. However, the piston may all along seal the bottom of the container, thereby preventing the fluid inside the container from flowing out. In some specific embodiments, the bottom 101 of the container may be sealed by the piston 300, so as to ensure that substance (liquid or solid) therein does not leak out of the bottom 101. In the process of moving inside the piston cavity body 201, the piston 300 keeps sealing the bottom 101 of the container. In some specific embodiments, a through hole 101 is disposed at the joint where the bottom of the container is connected with the pedestal. The container is communicated with the piston cavity body 201 of the pedestal by means of the through hole 101. More specifically, the through hole 101 is sealed by the piston 300. There are many sealing methods thereupon. For example, a seal groove is disposed on the piston, on the position of the corresponding seal groove there is provided with elastic sealing rings 318, 351 and 352. These elastic sealing rings are in contact with the internal wall of the piston cavity to form a liquid seal or a gas seal. In this way, the part between two sealing rings on the piston and the container form sealed space, and the liquid inside the container cannot flow out of the piston cavity body. In this way, a plurality of sealing regions is formed among the three sealing rings of the piston.

In some specific embodiments, the external surface 310 of the piston includes at least two regions: the first sealing region 311 and the second sealing region 312. These regions and the piston cavity body 201 constitute a sealing. In the process when the piston 300 is moving inside the piston cavity body 201, one of these regions is positioned at the through hole 101 through which the container 1 is communicated with the piston cavity body 201, for sealing the through hole 101 and then sealing the bottom of the container 1.

The piston 300 may move inside the piston cavity body 201. In a specific embodiment, inside the piston cavity body 201 the piston 300 has at least two positions: the first position and the second position. When the piston 300 is in the first position of the cavity body, as shown in FIG. 23, 4 or 5, the bottom 101 of the container is positioned on the first sealing region 311, more specifically, the through hole 101 at the bottom of the container is positioned on the first sealing region 311, and the space formed by the first sealing region 311 and the piston cavity body 201 is sealed, so that the container bottom and the through hole at the bottom are sealed, and the solid or liquid therein does not leak out of the piston cavity body 201. Similarly, when the piston 300 is in the second position of the piston cavity body 201, as shown in FIG. 26, the second sealing region 312 is positioned at the bottom 101 of the container, and the through hole 101 at the bottom is positioned within the second sealing region 312, and is sealed by enclosed space formed by the second region 312 and the piston cavity body 201. In some preferred embodiments, the sealing regions further include grooves. The first region 311 includes a first groove 313, and the second sealing region 312 includes a second groove 314. These

grooves

313 and 314 are used for receiving substances needing to be mixed in the invention, namely some solids or liquids. In addition, the

grooves

313 and 314 are also used for sealing the through hole at the bottom of the container. When the

grooves

313 and 314 are positioned at the through hole at the bottom of the container, the side walls of the

grooves

313 and 314 seal the through hole 101, so that the

grooves

313 and 314 are connected with the container bottom into a whole, meanwhile, solid substance 500 inside the groove is mixed with liquid substance 600 inside the container. As the piston moves, the solid (at the beginning the solid may be not in contact with the liquid, both of which is separated from each other) prestored in the

groove

313 or 314 may be mixed with the liquid inside the container in due time. In this way, the solid is dissolved into the liquid inside the container.

In some embodiments, the external surface 310 of the piston includes multiple sealing rings 318 and sealing lines 317. The external surface 310 of the piston is divided, by the sealing rings 318 and sealing lines 317, into at least two sealing regions. As shown in FIG. 21, the first sealing region 311 and the second sealing region 312 are formed by means of division by the sealing rings 318 and the sealing lines 317. Both the first sealing region 311 and the second sealing region 312 are positioned below the container bottom 101. By means of motion of the piston 300 inside the piston cavity body 201, the sealing

regions

311 and 312 successively arrive at the container bottom 101. Similarly, the

grooves

313 and 314 positioned within the sealing regions are respectively communicated with the through hole 101 at the bottom of the container and seal the through hole 101.

In addition to dividing into the first region 311 and the second region 312, the sealing rings 318 and the sealing lines 317 may also divide, on the external surface of the piston, into a third region, a fourth region……. Of course, the piston 300 moves inside the piston cavity body 201, and arrives at the first position, the second position, the third position, the fourth region……. When the piston 300 arrives at a certain specific position, the sealing region on the external surface 310 of the piston is positioned at the bottom 101 of the container and is communicated with the container 1. In this way, it is implemented that the solid or liquid inside the region and/or inside the groove of the region is mixed with the solid or liquid inside the container 1. In these regions, at the beginning the liquid or solid inside the container may be separated from each other without contact. By means of motion of the piston, they become in contact with each other from a separated state, for example, the solid is in contact with the liquid. In this way, some chemical substances that may react with each other or substances in different states may be in contact with each other if necessary. For example, influenza antigens need to be extracted when an influenza sample is collected, so that substances originally separated from each other are not in contact with each other unless the influenza sample needs to be processed, thereby forming fresh treating liquid.

It is a problem to be solved by the present invention how the piston 300 moves inside the piston cavity body 201. In some embodiments, the piston 300 includes a push rod 320 positioned inside the piston 300. By squeezing the push rod 320, the piston 300 is driven to push ahead inside the cavity body 201. In a more specific embodiment, the push rod 320 includes a rod shank 321 and a buckle structure connected to the rod shank； and the piston 300 includes a corresponding mechanism matching up with the buckle structure. By means of matching of the buckle structure and the corresponding mechanism, the push rod 320 may move relatively to the piston 300, or maintain a fixed relation.

Specifically, the push rod 320 has an initial position and an end position inside the piston 300. When the push rod 320 is in the initial position, the piston 300 has a smaller size and the push rod 320 can move relatively to the piston； when the push rod 320 is in the end position, the piston 300 has a larger size and the push rod 320 cannot move relatively to the piston 300. More specifically, when the push rod 320 is in the initial position, the buckle structure of the push rod 320 and the corresponding mechanism of the piston 300 are in an unfastened state, so that they can move relatively； when the push rod 320 is in the end position, the buckle structure of the push rod 320 and the corresponding mechanism of the piston 300 are in a fastened state, so that they cannot move relatively.

In addition, when the push rod 320 is in the initial position, the piston 300 cannot move relatively inside the piston cavity body 201； at the moment, the whole push rod 320 is positioned inside the piston 300 and can move relatively to the piston 300, the piston 300 is unable to move inside the piston cavity body 201, and at the moment the piston 300 is in the first position, as shown in FIG. 24. After the push rod 320 is pulled out of the piston 300 from the initial position and arrives at the end position, the push rod 320 is engaged and fixed to the piston 300, as shown in FIG. 25.Driven by the fixed push rod 320, the piston 300 may move back and forth inside the piston cavity body 201, so that the piston 300 arrives at the second position, as shown in FIG. 26.

In a specific embodiment, the buckle structure on the push rod 320 includes an elastic arm 322 and a lug 323 positioned at an outer side of a free end of the elastic arm. The corresponding mechanism of the piston 300 includes a channel 315 for receiving the elastic arm and a lockhole 316 corresponding to the lug. When the push rod 320 is in the initial position, the elastic arm 322 and the lug 323 of the push rod are compressed and received in the channel 315 of the corresponding mechanism inside the piston 300； when the push rod 320 is in the end position, the lug 323 at the free end of the elastic arm 322 of the push rod is buckled in the lockhole 316 of the corresponding mechanism inside the piston. When the push rod 320 is pulled out of the piston 300, the elastic arm 322 moves inside the channel 315 of the piston until the lug 323 on the elastic arm arrives at the lockhole 316 on the piston and is engaged by the lockhole. At the moment, the push rod 320 and the piston 300 are engaged and fixed.

The following describes in detail the method for mixing at least two substances by using the device 800 of the present invention. Specific steps of the method are as below:

1. Mixing two substances (two different liquids, or a solid and another solid)

a first substance (solid 500) is stored in the second region 312 on the external surface of the piston； more specifically, the solid 500 is stored in the second groove 314 of the second region；

the piston 300 with the solid 500 is disposed in the first position inside the piston cavity 201, the through hole 101 at the joint where the container 1 is communicated with the pedestal 200 is connected with the first region 311 on the external surface of the piston, and is enclosed and sealed by the first region 311； specifically, the through hole 101 is positioned inside the first groove 313, and is enclosed and sealed by the first groove 313 which is communicated with the container 1；

a second substance (liquid 600) is stored in the container 1 of the device；

the push rod 320 inside the piston 300 is pulled out of the channel 315 of the piston 300 until the push rod 320 moves from the initial position and arrives at the end position, i.e., the elastic arm 322 of the push rod is pulled out of the channel 315 of the piston until the lug 323 at the free end of the elastic arm arrives at the lockhole 316 on the piston and is engaged and fixed；

the handle 321 of the push rod is pushed, so that the piston 300 connected and fixed to the push rod 320 moves, inside the piston cavity 201, from the first position to the second position, the through hole 101 at the joint where the container 1 is communicated with the pedestal 200 is connected with the second region 312 on the external surface 110 of the piston body, and is enclosed and sealed by the second region 312. At the moment, the second sealing region further includes a second groove 314 in which the solid is positioned, and the through hole 101 is positioned inside the second groove 314, and is enclosed and sealed by the second groove 314； and

the container 1 is shaken so that the first substance (solid 500) is mixed with the second substance (liquid 600) .

2. Another method for mixing three substances includes the following specific steps:

a third substance is stored in the first region 311 on the external surface 310 of the piston； more specifically, the third substance is stored in the first groove 314 of the first region；

the piston 300 with the solid 500 and the third substance is disposed in the first position inside the piston cavity 201, the through hole 101 at the joint where the container 1 is communicated with the pedestal 200 is connected with the first region 311 on the external surface of the piston, and is enclosed and sealed by the first region 311； specifically, the through hole 101 is positioned inside the first groove 313, and is enclosed and sealed by the first groove 313, the third substance inside the first groove 313 is communicated with the container 1；

a second substance (liquid 600) is stored in the container 1 of the device；

the third substance is mixed with the second substance (liquid 600) ；

the container 1 is shaken so that the first substance (solid 500) and the second substance (liquid 600) are mixed with the third substance.

The present invention has been described in detail and illustrated so that those skilled in the art may make and use the present invention. Various alternatives, revisions and improvements apparently do not depart from the essence the scope of the present invention.

Those skilled in the art may easily understand that the present invention is very suitable for executing targets and tasks, and acquiring the results and advantages mentioned, and their inherent advantages. The embodiments as provided herein are representative and exemplary preferred embodiments, and are not intended to limit the scope of the present invention. Those skilled in the art may make some alterations for other purposes. All these alterations are contained in the spirit of the present invention and are defined within the scope of the claims.

To those skilled in the art, it is apparently very easy to perform different substitutions and alterations on what is disclosed in the present invention without departing from the scope and the spirit of the present invention.

All patents and publications as mentioned in the specification indicate that the level involved in the present invention is the level of ordinary skill in the art. All patents and publications are referenced in bibliographies in the same degree, just like that each of the publications is particularly and separately referenced in bibliographies.

Description of embodiments of the present invention may be put into practice in lack of an element or some elements not particularly disclosed in this paper, or of a limitation or some limitations. Therefore, for example, in each embodiment, any a term among “comprise” , “basically” and “consist of” may be replaced by other two terms. Terms and means of expression used in this paper are intended for description instead of limitation. However, this does not mean that use of these terms and means of expression may exclude any function as shown and description thereof or a part of equivalents. It is recognized that various modifications are possible within the scope of the claims of the present invention. Therefore, it is understood that although the present invention discloses preferred embodiments and optional functions, modified and changed concepts as disclosed herein may be resorted to those skilled in the art, and these modifications and changes are regarded as appended claims as defined within the scope of the present invention.

Other embodiments will be described in the following claims.

Claims

A mixing device, comprising a device body and a cover body covering and sealing the device body, wherein the cover body comprises a first cover body and a second cover body； and the first cover body is positioned inside the second cover body, and the first cover body may move relatively to the second cover body.
The mixing device according to claim 1, wherein the first cover body comprises a first position, a second position and a third position on the second cover body.
The mixing device according to claim 2, wherein when the first cover body is in the first position, the first cover body may rotate relatively to the second cover body； when the first cover body is in the second position, the first cover body may move up and down relatively to the second cover body； when the first cover body is in the third position, the first cover body is fixed relatively to the second cover body.
The mixing device according to any one of claims 1-3, wherein the first cover body and the second cover body are buckle structures.
The mixing device according to claim 4, wherein when the first cover body is in the first position and the second position, the first cover body is not engaged with the second cover body； and when the first cover body is in the third position, the first cover body is engaged and fixed to the second cover body.
The mixing device according to claim 5, wherein on the first cover body there is provided with a puncturing structure； and on a bottom of the second cover body there is provided with a sealing structure.
The mixing device according to claim 6, wherein when the first cover body is in the first position and the second position, the puncturing structure is not in contact with the sealing structure； and when the first cover body is in the third position, the puncturing structure on the first cover body punctures the sealing structure on the bottom of the second cover body.
The mixing device according to any one of claims 1-7, wherein the second cover body comprises a cavity body, the first cover body comprises a rod body, a lower part of the rod body is positioned inside the cavity body of the second cover body； and the rod body may move up and down inside the cavity body of the second cover body.
The mixing device according to claim 8, wherein at an upper part of the rod body of the first cover body there is provided with a convex rib； and on a side wall of the cavity body of the second cover body there is provided with a groove for receiving the convex rib.
The mixing device according to claim 9, wherein when the first cover body is in the first position, the convex rib on the rod body is positioned above a non-groove of the cavity body； when the first cover body is in the second position, the convex rib is positioned above the groove of the cavity body； and when the first cover body is in the third position, the convex rib is engaged and fixed inside the groove.
The mixing device according to claim 10, wherein there are two convex ribs symmetrically distributed on the rod body； and there are two grooves symmetrically positioned on the cavity body of the second cover body.
The mixing device according to claim 6, wherein the puncturing structure on the first cover body comprises a lug boss positioned on the bottom of the rod body； there may be one or more lug bosses.
The mixing device according to claim 6, wherein the sealing structure on the second cover body is a puncturable sealing membrane positioned on the bottom of the cavity body.
The mixing device according to claim 8, wherein the rod body of the first cover body seals the cavity body of the second cover body.
A device for storing and mixing liquid or solid, comprising an upper cover and a lower cover, wherein the upper cover may be movably connected with the lower cover； the lower cover comprises a sealing cavity body； and the upper cover comprises a puncturing and sealing structure for puncturing and sealing the sealing cavity body of the lower cover.
The device according to claim 15, wherein on the upper cover and the lower cover there is provided with a first position and a second position； when the upper cover is in the first position, the puncturing and sealing structure is positioned on an upper surface of the sealing cavity body and is not in contact with the upper surface； when the upper cover is in the process of moving from the first position to the second position, the puncturing and sealing structure is in contact with the upper surface of the sealing cavity body, punctures and seals the surface, and enters and squeezes the sealing cavity body, so that liquid in the sealing cavity body flows out； and when the upper cover is in the second position, the puncturing and sealing structure is totally inside the sealing cavity body.
The device according to claim 15 or 16, wherein the puncturing and sealing structure comprises a conical lug boss.
The device according to claim 17, wherein the puncturing and sealing structure further comprises a cylinder connected to an end of the conical lug boss.
The device according to claim 18, wherein a diameter of the cylinder is equal to an inside diameter of the sealing cavity body.
The device according to claim 19, wherein a length of the conical lug boss is smaller than one half of that of the sealing cavity body.
The device according to claim 17, wherein the puncturing and sealing structure further comprises a channel through which liquid inside the sealing cavity body flows into the upper cover.
The device according to claim 17, wherein an opening of the channel is positioned on the conical lug boss.
The device according to claim 22, wherein the upper surface of the sealing cavity body comprises a puncturable membrane or rubber.
The device according to claim 16, wherein the cover body comprises a clasp that prevents the upper cover and the lower cover from moving； and the clasp may be detached from the cover body.
A device for mixing at least two substances, comprising a pedestal and a container connected to the pedestal, wherein the pedestal comprises a piston cavity body and a piston positioned inside the piston cavity body； a bottom of the container is communicated with the piston cavity body on the pedestal and is sealed by the piston； and the piston may move inside the piston cavity body.
The device according to claim 25, wherein the bottom of the container comprises a through hole, and the container is communicated with the through hole and the piston cavity body.
The device according to claim 26, wherein an external surface of the piston comprises at least two sealing regions.
The device according to claim 27, wherein the through hole is positioned within the sealing region and is sealed by the sealing region.
The device according to claim 28, wherein each sealing region comprises a groove which is communicated with the through hole at the bottom of the container.
The device according to any one of claims 25-29, wherein the piston comprises at least a first position and a second position in the piston cavity body.
The device according to claim 30, wherein when the piston is in the first position, a first sealing region of the piston is positioned at the bottom of the container and seals the through hole at the bottom of the container.
The device according to claim 30, wherein when the piston is in the second position, a second sealing region of the piston is positioned at the bottom of the container and seals the through hole at the bottom of the container.
The device according to claim 28, wherein the surface of the piston comprises multiple sealing rings and sealing lines, and the piston is divided, by the sealing rings and the sealing lines, into at least two sealing regions.
The device according to any one of claims 25-33, wherein the piston comprises a push rod positioned inside the piston.
The device according to claim 34, wherein the push rod has an initial position and an end position inside the piston.
The device according to claim 34, wherein when the push rod is in the initial position, the piston cannot relatively move inside the piston cavity body； when the push rod is in the end position, driven by the push rod, the piston can move back and forth inside the piston cavity body.
The device according to claim 36, wherein when the pull rod is in the initial position, the piston has smaller size and the pull rod can move relatively to the piston； when the pull rod is in the end position, the piston has larger size and the pull rod cannot move relatively to the piston.
The device according to claim 36, wherein the push rod comprises a rod shank and a buckle structure connected to the rod shank； and the piston comprises a corresponding mechanism matching up with the buckle structure.
The device according to claim 38, wherein when the push rod is in the initial position, the buckle structure of the push rod and the corresponding mechanism of the piston are in an unfastened state, so that they can move relatively； when the push rod is in the end position, the buckle structure of the push rod and the corresponding mechanism of the piston are in a fastened state, so that they cannot move relatively.
The device according to claim 39, wherein the buckle structure comprises two elastic arms and a lug positioned at an outer side of a free end of the elastic arm.
The device according to claim 40, wherein the corresponding mechanism of the piston comprises a channel for receiving the elastic arm and a lockhole corresponding to the lug.