[go: up one dir, main page]

WO2013024024A3 - Quality assessment of induced pluripotent cells by referring to splicing signatures of pluripotency - Google Patents

Quality assessment of induced pluripotent cells by referring to splicing signatures of pluripotency Download PDF

Info

Publication number
WO2013024024A3
WO2013024024A3 PCT/EP2012/065681 EP2012065681W WO2013024024A3 WO 2013024024 A3 WO2013024024 A3 WO 2013024024A3 EP 2012065681 W EP2012065681 W EP 2012065681W WO 2013024024 A3 WO2013024024 A3 WO 2013024024A3
Authority
WO
WIPO (PCT)
Prior art keywords
splicing
pluripotency
signatures
referring
induced pluripotent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2012/065681
Other languages
French (fr)
Other versions
WO2013024024A2 (en
Inventor
Delphine DEBONO-LAGNEAUX
Jean-François DELEUZE
Marie-Christine Multon
Corinne Rocher
Emmanuel SPANAKIS
Gilbert Thill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi SA
Original Assignee
Sanofi SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi SA filed Critical Sanofi SA
Publication of WO2013024024A2 publication Critical patent/WO2013024024A2/en
Publication of WO2013024024A3 publication Critical patent/WO2013024024A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Transplantation (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention concerns a method of determining the pluripotency status of a cells comprising looking at the splicing signature in said cells, and correlating said splicing signature with the pluripotency status of the cells.
PCT/EP2012/065681 2011-08-12 2012-08-10 Quality assessment of induced pluripotent cells by referring to splicing signatures of pluripotency Ceased WO2013024024A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11306044 2011-08-12
EP11306044.6 2011-08-12

Publications (2)

Publication Number Publication Date
WO2013024024A2 WO2013024024A2 (en) 2013-02-21
WO2013024024A3 true WO2013024024A3 (en) 2013-04-11

Family

ID=46642550

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2012/065681 Ceased WO2013024024A2 (en) 2011-08-12 2012-08-10 Quality assessment of induced pluripotent cells by referring to splicing signatures of pluripotency

Country Status (2)

Country Link
AR (1) AR087491A1 (en)
WO (1) WO2013024024A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115885048A (en) * 2020-06-19 2023-03-31 富士胶片株式会社 Biomarker determination method and cell production method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010118525A1 (en) * 2009-04-14 2010-10-21 Socpra Sciences Santé Et Humaines S.E.C. Signature of secreted protein isoforms specific to ovarian cancer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010118525A1 (en) * 2009-04-14 2010-10-21 Socpra Sciences Santé Et Humaines S.E.C. Signature of secreted protein isoforms specific to ovarian cancer

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
A. R. GROSSO ET AL: "Tissue-specific splicing factor gene expression signatures", NUCLEIC ACIDS RESEARCH, vol. 36, no. 15, 1 January 2008 (2008-01-01), pages 4823 - 4832, XP055039471, ISSN: 0305-1048, DOI: 10.1093/nar/gkn463 *
CLARA Y. CHEONG ET AL: "Alternative Splicing in Self-Renewal of Embryonic Stem Cells", STEM CELLS INTERNATIONAL, vol. 2011, 9 January 2011 (2011-01-09), pages 1 - 8, XP055039539, ISSN: 1687-966X, DOI: 10.4061/2011/560261 *
DATABASE Geneseq [Online] 10 March 2005 (2005-03-10), "Human cartilage-related fibronectin EBD DNA fragment - SEQ ID 51.", XP002673403, retrieved from EBI accession no. GSN:ADV51120 Database accession no. ADV51120 *
DATABASE NUCLEOTIDE [online] MUNGALL,A.J. ET AL.: "Homo sapiens transmembrane protein 63B (TMEM63B), mRNA.", XP002689365, retrieved from NCBI Database accession no. NM_018426 *
GOPALAKRISHNA-PILLAI SAILESH ET AL: "A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells", PLOS ONE, vol. 6, no. 6, E20663, June 2011 (2011-06-01), pages 1 - 10, XP002689366, ISSN: 1932-6203 *
GUSTAVO S. GUIMARÃES ET AL: "Identification of candidates for tumor-specific alternative splicing in the thyroid", GENES, CHROMOSOMES AND CANCER, vol. 45, no. 6, 1 June 2006 (2006-06-01), pages 540 - 553, XP055047915, ISSN: 1045-2257, DOI: 10.1002/gcc.20316 *
JULIAN P VENABLES ET AL: "Cancer-associated regulation of alternative splicing", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 16, no. 6, 1 June 2009 (2009-06-01), pages 670 - 676, XP055039474, ISSN: 1545-9993, DOI: 10.1038/nsmb.1608 *
M. PRITSKER: "Diversification of stem cell molecular repertoire by alternative splicing", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 102, no. 40, 1 January 2005 (2005-01-01), pages 14290 - 14295, XP055039458, ISSN: 0027-8424, DOI: 10.1073/pnas.0502132102 *
MARTINA HALLEGGER ET AL: "Alternative splicing: global insights", FEBS JOURNAL, vol. 277, no. 4, 1 February 2010 (2010-02-01), pages 856 - 866, XP055039466, ISSN: 1742-464X, DOI: 10.1111/j.1742-4658.2009.07521.x *
MUNGALL A J ET AL: "The DNA sequence and analysis of human chromosome 6", NATURE: INTERNATIONAL WEEKLY JOURNAL OF SCIENCE, NATURE PUBLISHING GROUP, UNITED KINGDOM, vol. 425, no. 6960, 23 October 2003 (2003-10-23), pages 805 - 811, XP008129728, ISSN: 0028-0836 *
RAI B S GILL ET AL: "Sulf2 gene is alternatively spliced in mammalian developing and tumour tissues with functional implications", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 414, no. 3, 24 November 2011 (2011-11-24), pages 468 - 473, XP028328919, ISSN: 0006-291X, [retrieved on 20110924], DOI: 10.1016/J.BBRC.2011.09.088 *
SALOMONIS NATHAN ET AL: "Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 107, no. 23, June 2010 (2010-06-01), pages 10514 - 10519, XP002684311, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AR087491A1 (en) 2014-03-26
WO2013024024A2 (en) 2013-02-21

Similar Documents

Publication Publication Date Title
WO2013055698A3 (en) Payment device with account identifier
DK2559753T3 (en) Microorganism with improved L-valine productivity and method for producing L-valine using the same
EP3540100B8 (en) Fibronectin type iii repeat based protein scaffolds with alternative binding surfaces
BR112014006747A2 (en) fuel cell stack and fuel cell
IL248485B (en) Cartridge case on high strength polymer for idle and subsonic ammunition
CA2726756C (en) Energy consumption reporting and modification system
WO2013046060A9 (en) Paper and methods of making paper
EP2785825A4 (en) MICRO-INCUBATION SYSTEMS FOR MICROFLUIDIC CELL CULTURE AND ASSOCIATED METHODS
WO2013109856A3 (en) Methods of using fgf19 modulators
ZA201307923B (en) Methods and systems for assessing financial personality
GB201400417D0 (en) Detecting undesirable content on a social network
DK2701845T3 (en) PROCEDURE FOR REGULATING THE ROLLING PRESSURE IN A ROLLING PRESSURE
EP2769502A4 (en) Methods, systems and apparatus to facilitate client-based authentication
EP2783405A4 (en) EMBEDDED SEPARATORS, BATTERIES AND RELATED METHODS
PT2785683T (en) Inkt cell modulators and methods of using the same
SG194480A1 (en) Method of forming membranes with modified stress characteristics
BR112015008111A2 (en) genetically modified yeast cell, and method for the production of 3hp.
WO2011119986A3 (en) Methods for culturing and analyzing cells
MY171329A (en) Method for coating light alloy rims
EP2755746A4 (en) CONTACTORS, CARTRIDGES, ENHANCED ELEMENTS AND SYSTEMS, AND ASSOCIATED METHODS
BR112014027361A2 (en) fuel cell stack assembly, and method for assembling a fuel cell stack assembly
EP3065208A4 (en) Separator-fitted single fuel cell, and fuel cell stack
EP2905330A4 (en) Cell culturing member, and cell culturing method
BR112013030164A2 (en) fuel cell and process of making a fuel cell.
WO2012112458A3 (en) Compositions and methods for increasing reprogramming efficiency

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12745867

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 12745867

Country of ref document: EP

Kind code of ref document: A2