WO2012121462A1 - Method for producing hydrogen gas using thermococcus sp. strains - Google Patents
Method for producing hydrogen gas using thermococcus sp. strains Download PDFInfo
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- C01B3/02—Production of hydrogen or of gaseous mixtures containing a substantial proportion of hydrogen
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- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- the present invention relates to a method for producing hydrogen gas using a strain of the genus Thermococcus.
- Hydrogen energy has been spotlighted as an energy to replace fossil energy in the future because it does not emit substances that may adversely affect the environment such as carbon dioxide, NOx, and SOx while the calorific value per weight is three times higher than that of oil.
- Conventionally used hydrogen production methods include electrolysis of water, thermal cracking of natural gas or naphtha, or steam reforming.
- these methods again have a problem of making high-temperature and high-pressure conditions using fossil fuels, and generate a mixed gas containing carbon monoxide, which causes a difficult problem of removing carbon monoxide from such gases.
- the biological hydrogen production method using microorganisms does not need to make a high-temperature, high-pressure condition by inputting additional energy, and does not include carbon monoxide in the generated gas.
- Such biological hydrogen production methods can be broadly divided into using photosynthetic microorganisms and non-photosynthetic microorganisms (mainly anaerobic microorganisms). Examples of the former include the Republic of Korea Patent No. 10-0680624 "Hydrogen production method using the photosynthetic bacterium Rhodobacter spheroides strain excellent hydrogen production at high salt concentration" and the like.
- the present inventors have tried to develop a method for producing hydrogen using a strain of the genus Thermococcus in a high temperature environment.
- the present invention was completed by clarifying that hydrogen is generated when culturing a strain of thermococcus by adding carbon monoxide, formic acid or a salt thereof, or starch to the medium.
- an object of the present invention is to provide a method for producing hydrogen gas using strains of the genus Thermococcus.
- the present invention provides a method for producing hydrogen gas using a strain of the genus Thermococcus comprising the following steps:
- thermococcus strain in a culture medium to which (i) carbon monoxide, (ii) formic acid or its salt or (iii) starch is added under anaerobic conditions;
- step (b) obtaining the hydrogen gas generated by step (a).
- the present inventors endeavored to develop a method for producing hydrogen using a thermophilus strain, a highly thermophilic strain in a high temperature environment. When culturing the strain of Cocous was confirmed that hydrogen is generated.
- the medium used in step (a) may be used without limitation as long as it is a medium used to culture microbial strains in the art, but preferably, YPS medium is used.
- the present invention is a method capable of obtaining hydrogen very efficiently by using a strain of the genus Thermococcus.
- the present invention is a method for producing hydrogen through a bioconversion process when adding carbon monoxide (CO), formic acid or salts thereof, or starch to a medium in the thermococcus strain culture medium.
- CO carbon monoxide
- thermococcus strain used in the present invention is thermococcus gamma tolerance ( Thermococcus gammatolerans ; DSM 15229), Thermococcus barophyllus MP ( Thermococcus barophilus MP; DSM 11836 strains of any one can be used.
- step (a) in the present invention is carried out at a culture temperature of 50-90 ° C, more preferably at a culture temperature of 60-85 ° C, most preferably 78- It is carried out at a culture temperature of 82 °C.
- the present invention relates to a method for producing hydrogen gas using a strain of the genus Thermococcus.
- the present invention does not require high pressure conditions, can generate hydrogen at atmospheric pressure, and does not generate harmful by-products.
- the present invention has the advantage that it is possible to produce high purity hydrogen with high efficiency and to produce hydrogen even at high temperature, compared with the conventional technology of producing hydrogen using microorganisms.
- the present invention has an economic advantage that can be directly utilized to generate hydrogen by capturing high temperature carbon monoxide discharged from petroleum refining process without a separate cooling process, and can be usefully used in terms of air purification.
- the method for producing hydrogen according to the present invention enables the production of hydrogen using biomass.
- Techniques for producing carbon monoxide, formic acid or starch using biomass are currently available.
- the present invention is an important technique for producing hydrogen gas using inexpensive biomass.
- the method for producing hydrogen according to the present invention is a method for producing hydrogen, which is less susceptible to inhibition by high concentration substrates. Therefore, the hydrogen production efficiency is expected to be higher than the conventional hydrogen production method.
- % used to indicate the concentration of a particular substance is solid / solid (weight / weight)%, solid / liquid (weight / volume)%, and Liquid / liquid is (volume / volume)%.
- thermococcus gamma tolerance in a yeast extract-peptone-sulfur (YPS) medium in an anaerobic environment of 80 °C Thermococcus gammatolerans ; DSM 15229), Thermococcus barophyllus MP ( Thermococcus barophilus MP; DSM 11836) strains were cultured (Holden et al. 2001). The strains were purchased from DSMZ (www.dsmz.de), Germany.
- Tests on physiological properties include 1.0 ml trace element mix, 1 ml aqueous vitamin solution (Balch, WE, GE Fox, LJ Magrum, CR Woese, and RS Wolfe. 1979. Methanogens: reevaluation of a unique biological group.Microbiol. Rev 43: 260-296.), NaCl (30 gl. -One ) And yeast extract (0.5 g l) -One Modified medium 1 (Sokolova, T. G., C. Jeanthon, N. A Kostrikina, N. A. Chernyh, A. V. Lebedinsky, E. Stackebrandt, and E. A. Bonch-Osmolovskaya. 2004.
- Example 2 Analysis of the result by culturing the thermococcus strain
- Hydrogen gas was measured using a gas chromatograph HP 5890 series II (Hewlett Packard) equipped with an HP-PLOT Molesieve column (Agilent) and a TCD detector. Argon was used as the gas carrier. In order to quantify hydrogen gas, a gas calibration standard (Supleco) containing 1% (w / w) of each component (CO, CO 2 , H 2 , CH 4 and O 2 ) in nitrogen was used.
- Thermococcus gamma tolerance ( Thermococcus gammatolerans ; DSM 15229), Thermococcus barophyllus MP ( Thermococcus barophilus MP; DSM 11836) Hydrogen production rate was analyzed using a variety of energy sources to ensure that strains to produce hydrogen efficiently.
- thermococcus gamma tolerance ( Thermococcus gammatolerans ; DSM 15229), Thermococcus barophyllus MP ( Thermococcus barophilus MP; DSM 11836) All strains showed growth or produced hydrogen in a medium containing starch, CO, or formate in the absence of sulfur (Tables 1 and 2).
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Abstract
Description
본 발명은 써모코쿠스 속 균주를 이용한 수소 가스 생산 방법에 관한 것이다.The present invention relates to a method for producing hydrogen gas using a strain of the genus Thermococcus.
수소에너지는 중량당 발열량이 석유보다 3배 이상 높으면서도, 이산화탄소, NOx, SOx 등 환경에 악영향을 미칠 수 있는 물질들을 배출하지 않아, 장차 화석에너지를 대체할 에너지로써 각광받고 있다.Hydrogen energy has been spotlighted as an energy to replace fossil energy in the future because it does not emit substances that may adversely affect the environment such as carbon dioxide, NOx, and SOx while the calorific value per weight is three times higher than that of oil.
종래부터 사용된 수소 생산 방법에는 물의 전기분해, 천연가스나 나프타의 열분해 (thermal-cracking) 또는 수증기 개질법 (steam reforming) 등이 있다. 그러나 이러한 방법들은 다시 화석연료를 사용하여 고온, 고압 조건을 만들어야 하는 문제가 있으며, 일산화탄소를 포함한 혼합가스를 발생시키므로 그러한 가스로부터 일산화탄소를 제거하여야 하는 어려운 문제를 발생시킨다.Conventionally used hydrogen production methods include electrolysis of water, thermal cracking of natural gas or naphtha, or steam reforming. However, these methods again have a problem of making high-temperature and high-pressure conditions using fossil fuels, and generate a mixed gas containing carbon monoxide, which causes a difficult problem of removing carbon monoxide from such gases.
반면 미생물을 이용한 생물학적 수소 생산 방법은 별도의 에너지를 투입하여 고온, 고압 조건을 만들 필요가 없고, 생성된 가스에 일산화탄소를 포함하지 않는다는 장점이 있다. 이러한 생물학적 수소생산방법은 크게 광합성 미생물을 이용하는 것과 비-광합성 미생물(주로 혐기성 미생물)을 이용하는 것으로 나눠볼 수 있다. 전자에 속하는 예로 대한민국 등록특허 제10-0680624호 "높은 염분농도에서 수소생성능이 우수한 광합성 세균 로도박터 스페로이데스 균주를 이용한 수소생산방법" 등이 있다.On the other hand, the biological hydrogen production method using microorganisms does not need to make a high-temperature, high-pressure condition by inputting additional energy, and does not include carbon monoxide in the generated gas. Such biological hydrogen production methods can be broadly divided into using photosynthetic microorganisms and non-photosynthetic microorganisms (mainly anaerobic microorganisms). Examples of the former include the Republic of Korea Patent No. 10-0680624 "Hydrogen production method using the photosynthetic bacterium Rhodobacter spheroides strain excellent hydrogen production at high salt concentration" and the like.
그러나 빛을 에너지원으로 사용하는 광합성 세균들의 고농도 배양기술이 아직 충분히 개발되어 있지 않으며, 종래의 광합성 세균들은 높은 분압의 기질이 있을 경우 기질저해가 심하다는 단점이 있다. 또한, 이들은 빛이 존재하는 경우에만 수소생성능이 지속 될 수 있다는 문제점이 있다. However, high concentration culture technology of photosynthetic bacteria using light as an energy source has not yet been sufficiently developed, and conventional photosynthetic bacteria have a disadvantage of severe substrate inhibition when there is a high partial pressure substrate. In addition, they have a problem that hydrogen production performance can be continued only in the presence of light.
따라서, 유기 탄소를 이용하여 수소를 생산할 수 있는 미생물들을 이용하여 수소를 생산하려는 시도가 지속적으로 이루어지고 있으며, 그 예로 대한민국 등록특허 제10-0315663호 "사이트로박터속 균주 Y19 및 이에 의한 수소 생산", 대한민국 등록특허 제10-0315662호 "로도슈도모나스 팔루스트리스 P4 및 이에 의한 수소 생산" 등이 있다.Therefore, attempts have been made to produce hydrogen using microorganisms capable of producing hydrogen using organic carbon. For example, Korean Patent No. 10-0315663 "Sysitebacterium strain Y19 and hydrogen production by this." ", Republic of Korea Patent No. 10-0315662" Rhodoshudo Monas Palustasis P4 and thereby hydrogen production "and the like.
본 명세서 전체에 걸쳐 다수의 논문 및 특허문헌이 참조되고 그 인용이 표시되어 있다. 인용된 논문 및 특허문헌의 개시 내용은 그 전체로서 본 명세서에 참조로 삽입되어 본 발명이 속하는 기술 분야의 수준 및 본 발명의 내용이 보다 명확하게 설명된다.Throughout this specification, many papers and patent documents are referenced and their citations are indicated. The disclosures of cited papers and patent documents are incorporated herein by reference in their entirety, and the level of the technical field to which the present invention belongs and the contents of the present invention are more clearly explained.
본 발명자들은 고온의 환경에서 고호열성 균주인 써모코쿠스 속 균주를 이용하여 수소를 생산할 수 있는 방법을 개발하고자 노력하였다. 그 결과, 일산화탄소, 포름산 또는 이의 염, 또는 녹말을 배지에 첨가하여 써모코쿠스 속 균주를 배양하는 경우 수소가 발생하는 것을 규명함으로써, 본 발명을 완성하게 되었다.The present inventors have tried to develop a method for producing hydrogen using a strain of the genus Thermococcus in a high temperature environment. As a result, the present invention was completed by clarifying that hydrogen is generated when culturing a strain of thermococcus by adding carbon monoxide, formic acid or a salt thereof, or starch to the medium.
따라서, 본 발명의 목적은 써모코쿠스 속 균주를 이용한 수소 가스 생산 방법을 제공하는데 있다.Accordingly, an object of the present invention is to provide a method for producing hydrogen gas using strains of the genus Thermococcus.
본 발명의 다른 목적 및 이점은 하기의 발명의 상세한 설명, 청구범위 및 도면에 의해 보다 명확하게 된다.Other objects and advantages of the present invention will become apparent from the following detailed description, claims and drawings.
본 발명의 양태에 따르면, 본 발명은 다음 단계를 포함하는 써모코쿠스 속 균주를 이용한 수소 가스 생산 방법을 제공한다:According to an aspect of the present invention, the present invention provides a method for producing hydrogen gas using a strain of the genus Thermococcus comprising the following steps:
(a) 혐기성 조건하에서 (i) 일산화탄소, (ii) 포름산 또는 이의 염 또는 (iii) 녹말이 첨가된 배양액에 써모코쿠스 속 균주를 배양시키는 단계; 및(a) culturing a thermococcus strain in a culture medium to which (i) carbon monoxide, (ii) formic acid or its salt or (iii) starch is added under anaerobic conditions; And
(b) 상기 단계 (a)에 의하여 발생된 수소 가스를 수득하는 단계.(b) obtaining the hydrogen gas generated by step (a).
본 발명자들은 고온의 환경에서 고호열성 균주인 써모코쿠스 속 균주를 이용하여 수소를 생산할 수 있는 방법을 개발하고자 노력하였으며, 그 결과, 일산화탄소, 포르산 또는 이의 염, 또는 녹말을 배지에 첨가하여 써모코쿠스 속 균주를 배양하는 경우 수소가 발생하는 것을 규명하였다.The present inventors endeavored to develop a method for producing hydrogen using a thermophilus strain, a highly thermophilic strain in a high temperature environment. When culturing the strain of Cocous was confirmed that hydrogen is generated.
본 발명에서 단계 (a)에서 이용되는 배지는 당업계에서 미생물 균주를 배양하기 위하여 이용되는 배지라면 제한없이 이용이 가능하나, 바람직하게는 YPS 배지를 이용된다.In the present invention, the medium used in step (a) may be used without limitation as long as it is a medium used to culture microbial strains in the art, but preferably, YPS medium is used.
본 발명은 써모코쿠스 속 균주를 이용하여 수소를 매우 효율적으로 수득할 수 있는 방법이다.The present invention is a method capable of obtaining hydrogen very efficiently by using a strain of the genus Thermococcus.
보다 구체적으로, 본 발명은 써모코쿠스 속 균주 배양액에 일산화탄소(CO), 포름산 또는 이의 염, 또는 녹말을 배지에 첨가하는 경우 생물전환공정을 통하여 수소를 생산하는 방법이다. More specifically, the present invention is a method for producing hydrogen through a bioconversion process when adding carbon monoxide (CO), formic acid or salts thereof, or starch to a medium in the thermococcus strain culture medium.
본 발명의 바람직한 구현예에 따르면, 본 발명에서 이용하는 써모코쿠스 속 균주는 써모코쿠스 감마톨레란스(Thermococcus gammatolerans; DSM 15229), 써모코쿠스 바로필루스 MP (Thermococcus barophilus MP; DSM 11836) 균주 중 어느 하나의 균주를 이용할 수 있다.According to a preferred embodiment of the present invention, the thermococcus strain used in the present invention is thermococcus gamma tolerance (Thermococcus gammatolerans; DSM 15229), Thermococcus barophyllus MP (Thermococcus barophilus MP; DSM 11836 strains of any one can be used.
본 발명의 바람직한 구현예에 따르면, 본 발명에서의 단계 (a)는 50-90 ℃의 배양 온도에서 실시되며, 보다 바람직하게는 60-85℃의 배양 온도에서 실시되고, 가장 바람직하게는 78-82℃의 배양 온도에서 실시된다.According to a preferred embodiment of the invention, step (a) in the present invention is carried out at a culture temperature of 50-90 ° C, more preferably at a culture temperature of 60-85 ° C, most preferably 78- It is carried out at a culture temperature of 82 ℃.
본 발명의 특징 및 이점을 요약하면 다음과 같다:The features and advantages of the present invention are summarized as follows:
(i) 본 발명은 써모코쿠스 속 균주를 이용한 수소 가스 생산 방법에 관한 것이다.(i) The present invention relates to a method for producing hydrogen gas using a strain of the genus Thermococcus.
(ii) 본 발명은 종래의 화학적 생산 방법과 달리 고압 조건을 필요로 하지 않고, 상압 조건에서 수소를 발생시킬 수 있으며, 유해한 부산물을 발생시키지 않는다는 장점이 있다.(ii) Unlike the conventional chemical production method, the present invention does not require high pressure conditions, can generate hydrogen at atmospheric pressure, and does not generate harmful by-products.
(iii) 본 발명은 미생물을 이용하여 수소를 생산하는 종래의 기술과 비교하더라도 고순도의 수소를 고효율로 생산할 수 있고, 고온 조건에서도 수소를 생산할 수 있는 장점이 있다.(iii) The present invention has the advantage that it is possible to produce high purity hydrogen with high efficiency and to produce hydrogen even at high temperature, compared with the conventional technology of producing hydrogen using microorganisms.
(iv) 또한, 본 발명은 석유정제공정 등에서 배출되는 고온의 일산화탄소를 별도의 냉각과정 없이 바로 포획하여 수소생성에 활용할 수 있는 경제적 이점이 있으며, 공기정화의 측면에서도 유용하게 이용될 수 있다.(iv) In addition, the present invention has an economic advantage that can be directly utilized to generate hydrogen by capturing high temperature carbon monoxide discharged from petroleum refining process without a separate cooling process, and can be usefully used in terms of air purification.
(v) 본 발명에 의한 수소 생산 방법은 바이오매스(biomass)를 이용하여 수소를 생산할 수 있도록 한다. 바이오매스를 이용하여 일산화탄소, 포름산 또는 녹말을 제조할 수 있는 기술은 현재 가용하다. 그렇기 때문에 본 발명은 값싼 바이오매스를 이용하여 수소 가스를 생산하는 중요한 기술이다.(v) The method for producing hydrogen according to the present invention enables the production of hydrogen using biomass. Techniques for producing carbon monoxide, formic acid or starch using biomass are currently available. As such, the present invention is an important technique for producing hydrogen gas using inexpensive biomass.
(vi) 본 발명에 의한 수소 생산 방법은 고농도 기질에 의한 저해를 덜 받을 수 있는 수소생산 방법이다. 따라서 수소 생산 효율이 종래 수소 생산 방법보다 높은 것으로 예상된다.(vi) The method for producing hydrogen according to the present invention is a method for producing hydrogen, which is less susceptible to inhibition by high concentration substrates. Therefore, the hydrogen production efficiency is expected to be higher than the conventional hydrogen production method.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .
실시예Example
본 명세서 전체에 걸쳐, 특정 물질의 농도를 나타내기 위하여 사용되는 “%“는 별도의 언급이 없는 경우, 고체/고체는 (중량/중량) %, 고체/액체는 (중량/부피) %, 그리고 액체/액체는 (부피/부피) %이다.Throughout this specification, unless otherwise indicated, “%” used to indicate the concentration of a particular substance is solid / solid (weight / weight)%, solid / liquid (weight / volume)%, and Liquid / liquid is (volume / volume)%.
실시예 1: 써모코쿠스 속 균주 배양 Example 1 Strain Culture of the Genus Thermococcus
일반적인 배양조건으로서, 80℃의 혐기성 환경에서 YPS(yeast extract-peptone-sulfur) 배지에 써모코쿠스 감마톨레란스(Thermococcus gammatolerans; DSM 15229), 써모코쿠스 바로필루스 MP (Thermococcus barophilus MP; DSM 11836) 균주들을 배양시켰다(Holden et al. 2001). 위 균주들은 독일 DSMZ (www.dsmz.de)에서 구입하여 사용하였다. 생리적 특성들에 대한 시험은 1.0 ㎖ 트레이스 엘리먼트 믹스쳐, 1 ㎖ 비타민 수용액 (Balch, W. E., G. E. Fox, L. J. Magrum, C. R. Woese, and R. S. Wolfe. 1979. Methanogens: reevaluation of a unique biological group. Microbiol. Rev. 43:260-296.), NaCl (30 g l-1) 및 효모추출물(yeast extract) (0.5 g l-1)을 추가한 변형배지 1(Sokolova, T. G., C. Jeanthon, N. A Kostrikina, N. A. Chernyh, A. V. Lebedinsky, E. Stackebrandt, and E. A. Bonch-Osmolovskaya. 2004. The first evidence of anaerobic CO oxidation coupled with H2 production by a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. Extremophiles 8:317-323.)을 이용하여 수행하였다. pH는 NaOH를 사용하여 8.0에 맞췄다. 혐기적 조건에서 만든 배지를 25-㎖ 시럼 바틀(serum bottle)에 넣고, 나머지 가스층(15 ㎖)을 N2/CO2 (80:20, 1 bar) 또는 100% CO로 채웠다. 포름산염(formate)이나 녹말(starch)로 배양시키는 경우에는, 가압멸균(autoclaving)시키기 전에 각각 10 g/l의 포름산염나트륨(Sigma) 이나 5 g/l의 가용성 녹말(Sigma)을 배지에 첨가하였다. 생리적 시험을 위한 배양은 모두 80 ℃에서 2일간 수행하였다.As a general culture condition, thermococcus gamma tolerance (YPS) in a yeast extract-peptone-sulfur (YPS) medium in an anaerobic environment of 80 ℃Thermococcus gammatolerans; DSM 15229), Thermococcus barophyllus MP (Thermococcus barophilus MP; DSM 11836) strains were cultured (Holden et al. 2001). The strains were purchased from DSMZ (www.dsmz.de), Germany. Tests on physiological properties include 1.0 ml trace element mix, 1 ml aqueous vitamin solution (Balch, WE, GE Fox, LJ Magrum, CR Woese, and RS Wolfe. 1979. Methanogens: reevaluation of a unique biological group.Microbiol. Rev 43: 260-296.), NaCl (30 gl.-One) And yeast extract (0.5 g l)-OneModified medium 1 (Sokolova, T. G., C. Jeanthon, N. A Kostrikina, N. A. Chernyh, A. V. Lebedinsky, E. Stackebrandt, and E. A. Bonch-Osmolovskaya. 2004. The first evidence of anaerobic CO oxidation coupled with H2 production by a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent. Extremophiles 8: 317-323.). pH was adjusted to 8.0 using NaOH. The medium made under anaerobic conditions is placed in a 25-ml serum bottle and the remaining gas layer (15 ml) is placed in N.2/ CO2 (80:20, 1 bar) or 100% CO. When incubated with formate or starch, 10 g / l sodium formate (Sigma) or 5 g / l soluble starch (Sigma) is added to the medium before autoclaving. It was. All cultures for physiological testing were performed at 80 ° C. for 2 days.
실시예 2: 써모코쿠스 속 균주 배양을 통한 결과물 분석Example 2: Analysis of the result by culturing the thermococcus strain
<분석방법> < Method of Analysis>
수소가스는 HP-PLOT Molesieve column (Agilent) 및 TCD detector를 갖춘 gas chromatograph HP 5890 series Ⅱ(Hewlett Packard)를 이용하여 측정하였다. 아르곤을 가스 운반체로 사용하였다. 수소가스를 정량하기 위하여 질소에 각각의 성분(CO, CO2, H2, CH4 및 O2) 1%(w/w)이 포함된 Gas calibration standard (Supleco)를 사용하였다.Hydrogen gas was measured using a gas chromatograph HP 5890 series II (Hewlett Packard) equipped with an HP-PLOT Molesieve column (Agilent) and a TCD detector. Argon was used as the gas carrier. In order to quantify hydrogen gas, a gas calibration standard (Supleco) containing 1% (w / w) of each component (CO, CO 2 , H 2 , CH 4 and O 2 ) in nitrogen was used.
<다양한 물질들을 이용한 수소 생성> < Hydrogen production using various materials>
써모코쿠스 감마톨레란스(Thermococcus gammatolerans; DSM 15229), 써모코쿠스 바로필루스 MP (Thermococcus barophilus MP; DSM 11836) 균주들이 수소를 효율적으로 생산하게 하는지 확인하기 위하여, 다양한 에너지원을 이용하여 수소 생성율을 분석하였다. 그 결과, 써모코쿠스 감마톨레란스(Thermococcus gammatolerans; DSM 15229), 써모코쿠스 바로필루스 MP (Thermococcus barophilus MP; DSM 11836) 균주들 모두 황이 없는 조건에서 녹말, CO, 혹은 포름산염(formate)이 포함된 배지에서 성장을 나타내거나 수소를 생산하였다(표 1 및 표 2). Thermococcus gamma tolerance (Thermococcus gammatolerans; DSM 15229), Thermococcus barophyllus MP (Thermococcus barophilus MP; DSM 11836) Hydrogen production rate was analyzed using a variety of energy sources to ensure that strains to produce hydrogen efficiently. As a result, thermococcus gamma tolerance (Thermococcus gammatolerans; DSM 15229), Thermococcus barophyllus MP (Thermococcus barophilus MP; DSM 11836) All strains showed growth or produced hydrogen in a medium containing starch, CO, or formate in the absence of sulfur (Tables 1 and 2).
표 1
M, 변형 배지(modified medium) Ⅰ M, modified medium I
표 2
이상으로 본 발명의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.Having described the specific part of the present invention in detail, it is apparent to those skilled in the art that such a specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereto. Therefore, the substantial scope of the present invention will be defined by the appended claims and equivalents thereof.
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| KANAI, T. ET AL.: "Continuous hydrogen production by the hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1", J. BIOTECHNOL., vol. 116, no. 3, 2005, pages 271 - 282, XP004737500 * |
| KAPDAN, I. K. ET AL.: "Bio-hydrogen production from waste materials", ENZYME AND MICROBIAL TECHNOLOGY, vol. 38, no. 5, 2006, pages 569 - 582, XP005252242 * |
| SOKOLOVA, T. G. ET AL.: "Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park.", INT. J. SYST. EVOL. MICROBIOL., vol. 54, no. 6, 2004, pages 2353 - 2359 * |
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