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WO2012116992A2 - Utilisation orale - Google Patents

Utilisation orale Download PDF

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Publication number
WO2012116992A2
WO2012116992A2 PCT/EP2012/053374 EP2012053374W WO2012116992A2 WO 2012116992 A2 WO2012116992 A2 WO 2012116992A2 EP 2012053374 W EP2012053374 W EP 2012053374W WO 2012116992 A2 WO2012116992 A2 WO 2012116992A2
Authority
WO
WIPO (PCT)
Prior art keywords
skin
steviol
isosteviol
vitamin
oral composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2012/053374
Other languages
English (en)
Other versions
WO2012116992A3 (fr
Inventor
Regina Goralczyk
Remo GRÄUB
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of WO2012116992A2 publication Critical patent/WO2012116992A2/fr
Publication of WO2012116992A3 publication Critical patent/WO2012116992A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/04—Preparations for care of the skin for chemically tanning the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/36—Carboxylic acids; Salts or anhydrides thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A61K8/602—Glycosides, e.g. rutin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/92—Oral administration

Definitions

  • the invention relates to the use of an oral composition comprising Steviol or Isosteviol as skin tanning agent. It further relates methods of enhancing the natural skin tan by oral administration of an effective amount of Steviol or Isosteviol.
  • a tanned skin is always a sign of good health.
  • One method of obtaining a tan is to expose skin to UV radiation causing direct DNA damage to the skin, which the body naturally combats and seeks to repair.
  • the body In the process of repairing the damage and protect the skin, the body creates and releases the brown- colored pigment called melanin into the skin's cells, which gives the skin a darker tone.
  • Melanin is produced by cells called melanocytes and protects the body from direct and indirect DNA damage by absorbing an excess of solar radiation.
  • exposure to UV radiation may have detrimental health effects such as sunburn or even skin cancer many people prefer alternatives which can produce a tanning result without exposure to ultraviolet radiation.
  • an oral composition comprising Steviol or Isosteviol
  • Steviol or Isosteviol has the surprising benefit of activating the melanin formation in human skin melanocytes and can thus be used for sunless skin tanning.
  • the change in pigmentation i.e. increased melanin content
  • one embodiment of this invention is a method of enhancing the natural tan of an animal's including human's skin comprising administering an oral composition comprising Steviol or Isosteviol for a time sufficient and in an amount effective to enhance the skin tan, and observing or appreciating the result.
  • Another embodiment of this invention is the use of Steviol or Isosteviol in the manufacture of an oral composition for the enhancement of the natural skin tan.
  • the invention relates to a method of reducing the risk of skin damage due to ultraviolet radiation, comprising the step of periodically or intermittently ingesting Steviol or Isosteviol in a quantity and over a span of time sufficient to cause a measurable increase in a person's ability to withstand a fixed dosage of ultraviolet radiation without suffering erythema.
  • Another aspect of this invention comprises a method of
  • Enhancing the photoprotection to both UV A and UV B induced skin damage comprising administering an oral composition comprising Steviol or Isosteviol.
  • Steviol or Isosteviol is used in a cosmetic oral nutraceutical or food composition for the above advantages involving skin tanning.
  • the oral composition comprising Steviol or Isosteviol can also provide preventive or therapeutic benefits among at least some patients who suffer from, or who are at elevated risk of, various other types of skin conditions, syndromes, or diseases. This includes patients who suffer from albinism, porphyria, or skin blotching or other lesions, and among patients taking certain types of antibiotics that increase their sensitivity to sunlight.
  • the invention also relates to a method of preventing photosensitivity in people with inherited erythropoietic protoporphyria and/ or treatment of vitiligo said method comprising administering an oral composition comprising Steviol or Isosteviol.
  • compositions according to the invention are especially attractive, since many people, have a special interest in cosmetic treatments considered as "natural” with mild effects and without major side effects.
  • Steviol or Isosteviol has further advantages in that it is easy to use, do not leave any residue on the skin, and dosages are easier to control than in topical formulations.
  • Steviol [CAS number: 471-80-7] may be obtained by enzymatic hydrolysis of stevioside according to methods described in the literature. It can for example be prepared by chemical synthesis such as e.g.: by deglycosylation of the respective glucosides (e.g.: stevioside or rebaudiosides A and C) [see e.g. Bulletin de la Societe de Chimie Bitechnik (1931), 13 781-96: The sweet principle in Kaa-he-e (Stevia rebaudiana. Bertoni). II. Hydrolysis of stevioside by enzymes. III. Steviol by enzymic hydrolysis and isosteviol by acid hydrolysis].
  • glucosides e.g.: stevioside or rebaudiosides A and C
  • Isosteviol [CAS: 27975-19-5] may be obtained by acid hydrolysis of stevioside according to methods described in the literature [see e.g. Bulletin de la Societe de Chimie Bitechnik (1931), 13 781-96: The sweet principle in Kaa-he-e (Stevia rebaudiana. Bertoni). II. Hydrolysis of stevioside by enzymes. III. Steviol by enzymic hydrolysis and isosteviol by acid hydrolysis]. In all embodiments of the invention preferably Steviol is used.
  • the oral composition is a nutraceutical composition further comprising a nutraceutically acceptable carrier.
  • the animal is a human.
  • skin means both skin of a human and of an animal.
  • skin tanning agent refers to agents who result in or support a darkening of the skin color which may be the result of an increased skin pigmentation (by an increased melanin formation) or other means (e.g. coloration of the skin by a pigment).
  • oral composition comprises any composition which is suitable for oral administration.
  • the term "nutraceutical composition” as used herein includes food products, foodstuffs, dietary supplements, nutritional supplements or a supplement composition for a food product or a foodstuff, including beverages (e.g., but not limited to, sports beverages, functional waters, juices, smoothies; instant drinks), soups, dairy products (e.g., but not limited to, single shot yogurt drinks), nutritional bars, and spreads.
  • beverages e.g., but not limited to, sports beverages, functional waters, juices, smoothies; instant drinks
  • soups e.g., but not limited to, single shot yogurt drinks
  • nutritional bars e.g., but not limited to, single shot yogurt drinks
  • food product refers to any food or feed suitable for consumption by humans or animals.
  • dietary supplement refers to a small amount of a compound for supplementation of a human or animal diet packaged in single or multiple dose units. Dietary supplements do not generally provide significant amounts of calories but may contain other micronutrients (e.g. vitamins or minerals).
  • nutritional supplement refers to a composition comprising a dietary supplement in combination with a source of calories.
  • nutritional supplements are meal replacements or supplements (e.g. nutrient or energy bars or nutrient beverages or concentrates).
  • Preventing as used herein is not intended to mean that the event will never occur, but means delaying the onset of the condition or event, and lessening the severity of the condition or event when it does occur.
  • “Chronic administration” is meant to convey that administration of the active ingredient regularly occurs over an extended period of time, for example once or twice per day for a time of at least about two weeks, preferably for at least one month, and more preferably at least two months.
  • the regular administration can be every two days, every three days, or once per week or twice per week.
  • Extended period of time means substantially daily for a period of time of at least about two weeks, preferably at least about a month, and even more preferably for at least about two months.
  • Observing or “appreciating” may be done by either the individual who ingests the active ingredient, or may be done by a third party.
  • the post-administration condition may be compared with the pre-administration condition and analyzed either using a standard test, or by subjective analysis.
  • the present invention relates to the use of oral Steviol or Isosteviol containing compositions in skin care, particularly for tanning of the skin. It also relates to orally administered skin care compositions, particularly for the tanning of the skin comprising Steviol or Isosteviol and a carrier conventionally used for oral compositions. Furthermore, the invention relates to a method of tanning the skin which comprises administering orally to a mammal, in particular a human, in need of such treatment a composition comprising an effective amount of Steviol or Isosteviol and observing the skin tan.
  • Another aspect of this invention is a supplement especially designed for skin tanning and/ or sun protection which comprises Steviol or Isosteviol. The humans should eat this supplement daily for at least one month, and preferably for at least two months prior to the sun exposure in order for its skin to be optimal tanned.
  • the supplement is in the form of a tablet or a capsule.
  • Steviol or Isosteviol can be combined with any other known or hereafter- discovered active ingredients that are believed to be helpful in inducing a skin-darkening response when ingested orally.
  • skin tanning agents might include, as examples, carotenoids such as lutein, lycopene, astaxanthin, canthaxanthin, zeaxanthin or beta- carotene as well as melanocyte-stimulating hormones and analogs or active portions thereof, tyrosine such as particularly L-tyrosine, copper, vitamin E and D and/or any or all of the ingredients listed in U.S. Pat. No.
  • 6,254,898 (Bragaglia 2001), which include green tea extract, lipoic acid, and selenomethionine.
  • Particularly preferred according to the invention is the use of Steviol or Isosteviol in combination with at least one further agent selected from the group of beta-carotene, lycopene, lutein, L-tyrosine and/ or canthaxanthin, vitamin E, vitamin D and copper.
  • the additional agent is preferably used in effective dosage levels of 0.50-250 mg/day such as 1.0 to 180 mg/ day in order to effectively support the darkening of the skin color.
  • the Steviol or Isosteviol is administered to a non-human animal, which is preferably a mammal.
  • a non-human animal which is preferably a mammal.
  • the benefits of this invention are not limited to mammals, and may be extended to other animals such as birds, or fish, or other animals where an increased skin coloration is of interest.
  • the bird may be poultry, especially ones which are entered in show competitions.
  • Fish, for example koi and the like can also benefit from enhanced coloration.
  • the non-human animal is a mammal, such as a companion animal (dog, cat, ferret) or an animal which is shown in competition (such as dogs, horses, cats, rabbits and other farm animals).
  • compositions of this invention will enhance the tan of the animal's skin.
  • another aspect of this invention is a veterinary nutraceutical or foodstuff containing a skin tan-enhancing amount of Steviol or Isosteviol.
  • Another aspect of this invention is a supplement especially designed for a show animal which comprises Steviol or Isosteviol.
  • the animal should be fed this supplement daily for at least one month, and preferably for at least two months prior to the competition in order for its skin to be at its optimal tan condition.
  • the supplement is in the form of a treat or chew.
  • Food products or foodstuffs are, for example, beverages such as non-alcoholic and alcoholic drinks as well as liquid preparations to be added to drinking water and liquid food.
  • Non-alcoholic drinks are for instance: soft drinks, sport drinks, fruit juices, (e.g. orange juice, apple juice and grapefruit juice); lemonades, teas, near-water drinks, milk and other dairy drinks (e.g. yoghurt drinks) and diet drinks.
  • food products or foodstuffs refer to solid or semi-solid foods comprising the composition according to the invention. These forms can include, but are not limited to, baked goods such as: cakes and cookies, puddings, dairy products, confections, snack foods, or frozen confections or novelties (e.g.
  • ice cream, milk shakes prepared frozen meals, candy, snack products (e.g. potato crisps/chips), liquid food such as soups, spreads, sauces, salad dressings, prepared meat products, cheese, yogurt and any other fat- or oil- containing foods, and food ingredients (e.g. wheat flour).
  • the food product may be a prepared and packaged food (e.g. mayonnaise, salad dressing, bread, or cheese food).
  • Dietary supplements of the present invention are formulated for oral delivery.
  • the ingredients of the dietary supplement of this invention are contained in acceptable excipients and/or carriers for oral consumption. The actual form of the carrier, and thus, the dietary supplement itself, is not critical.
  • the carrier may be a liquid, gel, gelcap, capsule, powder, solid tablet (coated or non-coated), tea, or the like.
  • the dietary supplement is preferably in the form of a tablet or capsule and most preferably in the form of a hard (shell) gelatine capsule.
  • Suitable excipients and/or carriers include maltodextrin, calcium carbonate, dicalcium phosphate, tricalcium phosphate, microcrystalline cellulose, dextrose, rice flour, magnesium stearate, stearic acid, croscarmellose sodium, sodium starch glycolate, crospovidone, sucrose, vegetable gums, lactose, methylcellulose, povidone, carboxymethylcellulose, corn starch, and the like (including mixtures thereof).
  • Preferred carriers include calcium carbonate, magnesium stearate, maltodextrin, and mixtures thereof.
  • the various ingredients and the excipient and/or carrier are mixed and formed into the desired form using conventional techniques.
  • the tablet or capsule of the present invention may be coated with an enteric coating that dissolves at a pH of about 6.0 to 7.0.
  • a suitable enteric coating that dissolves in the small intestine but not in the stomach is cellulose acetate phthalate. Further details on techniques for formulation and administration may be found in the latest edition of Remington's Pharmaceutical Sciences (Maack Publishing Co., Easton, PA).
  • the dietary supplement is provided as a powder or liquid suitable for adding by the consumer to a food or beverage.
  • the dietary supplement can be administered to an individual in the form of a powder, for instance to be used by mixing into a beverage, or by stirring into a semi-solid food such as a pudding, topping, sauce, puree, cooked cereal, or salad dressing, for instance, or by otherwise adding to a food, e.g. enclosed in caps of food or beverage containers for release immediately before consumption.
  • the dietary supplement may comprise one or more inert ingredients, especially if it is desirable to limit the number of calories added to the diet by the dietary supplement.
  • the dietary supplement of the present invention may also contain optional ingredients including, for example, herbs, vitamins, minerals, enhancers, colorants, sweeteners, flavourings, inert ingredients, and the like.
  • the dietary supplements further comprise vitamins and minerals including, but not limited to, calcium phosphate or acetate, tribasic; potassium phosphate, dibasic; magnesium sulphate or oxide; salt (sodium chloride); potassium chloride or acetate; ascorbic acid; ferric orthophosphate; niacinamide; zinc sulphate or oxide; calcium pantothenate; copper gluconate; riboflavin; beta-carotene; pyridoxine hydrochloride; thiamine mononitrate; folic acid; biotin; chromium chloride or picolonate; potassium iodide; sodium selenate; sodium molybdate; phylloquinone; vitamin D3; cyanocobalamin; sodium selenite; copper sulphate; vitamin A; vitamin C; inositol; potassium iodide.
  • vitamins and minerals including, but not limited to, calcium phosphate or acetate, tribasic; potassium phosphate, di
  • the present invention provides nutritional supplements (e.g. energy bars or meal replacement bars or beverages) comprising the composition according to the invention.
  • the nutritional supplement may serve as meal or snack replacement and generally provide nutrient calories.
  • the nutritional supplements provide carbohydrates, proteins, and fats in balanced amounts.
  • the nutritional supplement can further comprise carbohydrate, simple, medium chain length, or polysaccharides, or a combination thereof.
  • a simple sugar can be chosen for desirable organoleptic properties.
  • Uncooked cornstarch is one example of a complex carbohydrate.
  • the nutritional supplement contains, in one embodiment, combinations of sources of carbohydrate of three levels of chain length (simple, medium and complex; e.g. sucrose, maltodextrins, and uncooked cornstarch).
  • Sources of protein to be incorporated into the nutritional supplement of the invention can be any suitable protein utilized in nutritional formulations and can include whey protein, whey protein concentrate, whey powder, egg, soy flour, soy milk, soy protein, soy protein isolate, caseinate (e.g. sodium caseinate, sodium calcium caseinate, calcium caseinate, potassium caseinate), animal and vegetable protein and hydrolysates or mixtures thereof.
  • caseinate e.g. sodium caseinate, sodium calcium caseinate, calcium caseinate, potassium caseinate
  • the biological value of the protein should be considered first, with the highest biological values being found in caseinate, whey, lactalbumin, egg albumin and whole egg proteins.
  • the protein is a combination of whey protein concentrate and calcium caseinate. These proteins have high biological value; that is, they have a high proportion of the essential amino acids. See Modern Nutrition in Health and Disease, Eighth Edition, Lea & Febiger, publishers, 1986, especially Volume 1 , pages 30-32
  • the nutritional supplement can also contain other ingredients, such as one or a combination of other vitamins, minerals, antioxidants, fibers and other dietary supplements (e.g. protein, amino acids, choline, lecithin, omega-3 fatty acids). Selection of one or several of these ingredients is a matter of formulation, design, consumer and end-user preference.
  • the amounts of these ingredients added to the dietary supplements of this invention are readily known to the skilled artisan. Guidance to such amounts can be provided by the U.S. RDA doses for children and adults.
  • vitamins and minerals that can be added include, but are not limited to, calcium phosphate or acetate, tribasic; potassium phosphate, dibasic; magnesium sulphate or oxide; salt (sodium chloride); potassium chloride or acetate; ascorbic acid; ferric orthophosphate; niacinamide; zinc sulphate or oxide; calcium pantothenate; copper gluconate; riboflavin; beta-carotene; pyridoxine hydrochloride; thiamine mononitrate; folic acid; biotin; chromium chloride or picolonate; potassium iodide; sodium selenate; sodium molybdate; phylloquinone; vitamin D3; cyanocobalamin; sodium selenite; copper sulphate; vitamin A; vitamin C; Vitamin E, inositol; and potassium iodide.
  • a multi-vitamin and mineral supplement may be added to the nutraceutical compositions of the present invention to obtain an adequate amount of an essential nutrient, which is missing in some diets.
  • the multi-vitamin and mineral supplement may also be useful for disease prevention and protection against nutritional losses and deficiencies due to lifestyle patterns.
  • the nutritional supplement can be provided in a variety of forms, and by a variety of production methods.
  • the liquid ingredients are cooked; the dry ingredients are added with the liquid ingredients in a mixer and mixed until the dough phase is reached; the dough is put into an extruder, and extruded; the extruded dough is cut into appropriate lengths; and the product is cooled.
  • the bars may contain other nutrients and fillers to enhance taste, in addition to the ingredients specifically listed herein.
  • flavourings can be in the form of flavored extracts, volatile oils, chocolate flavourings, peanut butter flavoring, cookie crumbs, crisp rice, vanilla or any commercially available flavoring.
  • flavourings include, but are not limited to: pure anise extract, imitation banana extract, imitation cherry extract, chocolate extract, pure lemon extract, pure orange extract, pure peppermint extract, imitation pineapple extract, imitation rum extract, imitation strawberry extract, or pure vanilla extract; or volatile oils, such as lemon oil, bay oil, bergamot oil, cedarwood oil, walnut oil, cherry oil, cinnamon oil, clove oil, or peppermint oil; peanut butter, chocolate flavoring, vanilla cookie crumb, butterscotch or toffee.
  • the dietary supplement contains cocoa or chocolate.
  • Emulsifiers may be added for stability of the nutraceutical compositions.
  • suitable emulsifiers include, but are not limited to, lecithin (e.g.
  • the nutraceutical composition can contain natural or artificial (preferably low calorie) sweeteners, such as, but not limited to: saccharides, cyclamates, aspartamine, aspartame, acesulfame K, and/or sorbitol. Such artificial sweeteners can be desirable if the nutritional supplement is intended to be consumed by an overweight or obese individual, or an individual with type II diabetes who is prone to hyperglycaemia.
  • the preferred dosages range from 0.01 to 100 mg/kg body weight per day, preferably 0.1 to 50 mg/kg body weight per day, and more preferably from 0.5 to 25 mg/kg body weight per day, and/or 0.5 to 10 mg/kg body weight per day.
  • the preferred daily dosage of the subject composition as specified above may be administered in the form of one or more dosage units such as, e.g., a tablet. Most preferably the daily dosage of the subject composition is provided in the form of one dosage unit taken twice daily, for a total of two dosage units a day, or in the form of two dosage units taken twice daily, for a total of four dosage units a day. Compared to taking the total daily dose once a day, twice daily dosing of half the total daily dose in one or more dosage units per dose provides improved absorption and better maintenance of blood levels of the essential ingredients.
  • the Steviol or Isosteviol containing nutraceutical composition is eaten on a regular basis, i.e. at least daily for a sustained period of time (i.e. at least one week, preferably at least two weeks, and more preferably for at least three weeks, or at least one or two months) until the skin tan enhancement is noted. After this time, the consumer may choose to lessen the dosage.
  • Example 1 Induction of melanin synthesis by Steviol in human epidermal melanocytes Quantification of melanin synthesis in cell culture:
  • HEMn-MP Normal human melanocytes NHM (HEMn-MP, Clonetics) were seeded in 96 well cell culture plates and grown to sub-confluence for two days in a mixture of M2-Medium (Clonetics) and Medium (Promocell). Culture medium was exchanged with Culture Medium containing Steviol (96%) and melanogenesis progressed for another three days at 37°C with another medium exchange on day two. Including cell layer and culture supernatant the total melanin was extracted using 1.7M KOH with vigorous shaking at RT. The total melanin content was determined at 405 nm in an absorbance plate reader versus a control (100%)
  • Glycyrrhizin is used as a positive control since it is a known inducer of melanogenesis. Steviol shows a clear dose dependent positive effect on the production of melanin in human melanocytes. Furthermore, as can be retrieved from the results, steviol is about 1000 times more potent than the positive control Glycyrrhizin.
  • Steviol, microcrystalline cellulose, and Si0 2 are mixed in a tumbler mixer for 10 min. Then, lactose is added and the composition is mixed for a further 10 min. Crospovidone is combined with the other ingredients and mixed for 10 min. Finally, Mg-stearate is added to the other components and mixed for another 2 min. The mixture is compressed to tablets. One tablet per day is taken in the morning with breakfast.
  • Steviol, lactose and Si0 2 are mixed in a tumbler mixer for 15 minutes and then filled into capsules.
  • Two capsules per day for 3 months may be administered to a human adult for increasing the skin tan.
  • the dose can be increased on demand to up to 3 capsules per day.
  • 1 capsule can be taken on a regular basis.
  • the ready-to-drink soft drink contains ca. 15 mg enriched steviol extract per serving (250 ml). As a regular skin tanning supporting drink, 2 servings per day (500ml) may be drunk.
  • the enriched steviol is premixed with skimmed milk powder and placed in a planetary bowl mixer. Cornflakes and rice crispies are added and the total is mixed gently. Then the dried and cut apples are added.
  • water and salt are mixed in the amounts given above (solution 1).
  • glucose-, invert sugar- and sorbitol- syrups are mixed in the amounts given above (solution 2).
  • the fat phase constitutes a mixture of baking fat, palm kernel fat, lecithin and emulsifier.
  • Solution 1 is heated to 110°C.
  • Solution 2 is heated to 113°C and then cooled in a cold water bath. Subsequently, solutions 1 and 2 are combined.
  • the fat phase is melted at 75°C in a water bath, then added to the combined mixture of solutions 1 and 2.
  • Apple flavor and citric acid are added to the liquid sugar/fat mix.
  • the liquid mass is added to the dry ingredients and mixed well in the planetary bowl mixer.
  • the mass is put on a marble plate and rolled to the desired thickness.
  • the mass is cooled down to room temperature and cut into pieces.
  • the non- baked cereal bar contains ca. 10 mg steviol per serving (30 g). To support a natural skin tan, 1-2 cereal bars may be eaten per day.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Emergency Medicine (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

La présente invention concerne l'utilisation d'une composition orale comprenant du stéviol ou de l'isostéviol en tant qu'agent de bronzage de la peau. L'invention porte en outre sur des procédés d'amélioration du bronzage naturel de la peau, par l'administration orale d'une quantité efficace de stéviol ou d'isostéviol.
PCT/EP2012/053374 2011-03-01 2012-02-28 Utilisation orale Ceased WO2012116992A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11156451.4 2011-03-01
EP11156451 2011-03-01

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WO2012116992A2 true WO2012116992A2 (fr) 2012-09-07
WO2012116992A3 WO2012116992A3 (fr) 2013-01-10

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012116990A3 (fr) * 2011-03-01 2014-05-01 Dsm Ip Assets B.V. Nouvelle utilisation
WO2019154910A1 (fr) * 2018-02-07 2019-08-15 Ubeauty Global Composition de bronzage
CN113476321A (zh) * 2021-07-07 2021-10-08 珠海沅芷健康科技有限公司 一种用异甜菊醇制备皮肤美白和抗黑色素生成的化妆品制剂的应用
GR1010258B (el) * 2021-07-08 2022-06-27 ΙΟΥΛΙΑ ΚΑΙ ΕΙΡΗΝΗ ΤΣΕΤΗ ΦΑΡΜΑΚΕΥΤΙΚΑ ΕΡΓΑΣΤΗΡΙΑ ΑΒΕΕ με δ.τ. "INTERMED ΑΒΕΕ", Συνθεση που περιεχει καροτενοειδη, βιταμινες, συνενζυμο q10, αμινοξεα και μεταλλα για χρηση ως αυτομαυριστικο χωρις εκθεση στον ηλιο

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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WO2012116990A3 (fr) * 2011-03-01 2014-05-01 Dsm Ip Assets B.V. Nouvelle utilisation
WO2019154910A1 (fr) * 2018-02-07 2019-08-15 Ubeauty Global Composition de bronzage
CN113476321A (zh) * 2021-07-07 2021-10-08 珠海沅芷健康科技有限公司 一种用异甜菊醇制备皮肤美白和抗黑色素生成的化妆品制剂的应用
CN113476321B (zh) * 2021-07-07 2023-09-22 珠海沅芷健康科技有限公司 一种用异甜菊醇制备皮肤美白和抗黑色素生成的化妆品制剂的应用
GR1010258B (el) * 2021-07-08 2022-06-27 ΙΟΥΛΙΑ ΚΑΙ ΕΙΡΗΝΗ ΤΣΕΤΗ ΦΑΡΜΑΚΕΥΤΙΚΑ ΕΡΓΑΣΤΗΡΙΑ ΑΒΕΕ με δ.τ. "INTERMED ΑΒΕΕ", Συνθεση που περιεχει καροτενοειδη, βιταμινες, συνενζυμο q10, αμινοξεα και μεταλλα για χρηση ως αυτομαυριστικο χωρις εκθεση στον ηλιο

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