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WO2012158007A1 - Dispositif laser pour mesurer l'épaisseur de l'épiderme - Google Patents

Dispositif laser pour mesurer l'épaisseur de l'épiderme Download PDF

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Publication number
WO2012158007A1
WO2012158007A1 PCT/MX2011/000061 MX2011000061W WO2012158007A1 WO 2012158007 A1 WO2012158007 A1 WO 2012158007A1 MX 2011000061 W MX2011000061 W MX 2011000061W WO 2012158007 A1 WO2012158007 A1 WO 2012158007A1
Authority
WO
WIPO (PCT)
Prior art keywords
thickness
epidermis
fibres
light
fiber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/MX2011/000061
Other languages
English (en)
Spanish (es)
Inventor
Luis Vidal Ponce Cabrera
Miguel Ángel ARRONTE GARCÍA
Eduardo Marcelo DE POSADA PIÑAN
Teresa Flores Reyes
Eugenio RODRIGUEZ GONZALEZ
Adrian Fermín PEÑA DELGADO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INSTITUTO POLITECNICO NACIONAL
Original Assignee
INSTITUTO POLITECNICO NACIONAL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INSTITUTO POLITECNICO NACIONAL filed Critical INSTITUTO POLITECNICO NACIONAL
Priority to PCT/MX2011/000061 priority Critical patent/WO2012158007A1/fr
Priority to MX2013013301A priority patent/MX343805B/es
Publication of WO2012158007A1 publication Critical patent/WO2012158007A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/103—Measuring devices for testing the shape, pattern, colour, size or movement of the body or parts thereof, for diagnostic purposes
    • A61B5/107—Measuring physical dimensions, e.g. size of the entire body or parts thereof
    • A61B5/1072—Measuring physical dimensions, e.g. size of the entire body or parts thereof measuring distances on the body, e.g. measuring length, height or thickness
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/103—Measuring devices for testing the shape, pattern, colour, size or movement of the body or parts thereof, for diagnostic purposes
    • A61B5/107—Measuring physical dimensions, e.g. size of the entire body or parts thereof
    • A61B5/1079—Measuring physical dimensions, e.g. size of the entire body or parts thereof using optical or photographic means
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
    • A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis

Definitions

  • the present invention corresponds to an application device in the field of biological sciences, designed and constructed with the purpose of making measurements to estimate the thickness of the epidermis, making use of the laser light affected and transmitted through the skin in relation to to preset transmittance models in vivo.
  • optical techniques to solve problems in medicine is a field that is experiencing high growth. These applications can be considered as therapeutic or diagnostic. Within the diagnostic applications, the process of light-matter interaction is considered non-destructive and its main objective is to study the physiology or pathology of the tissue. There are multiple applications in medicine where it is necessary to determine the thickness of the epidermis.
  • the techniques for measuring the thickness of the epidermis are divided into two groups: invasive (destructive) techniques, and non-invasive (non-destructive) techniques.
  • Invasive techniques require thin sections obtained by biopsies and prepared using the parafin-formalin preservation method to be analyzed with the help of an optical microscope. Among its characteristics, it is highlighted that it is the one that delivers the most information in the sample, has a resolution ⁇ 1 ⁇ in deep tissues. Unfortunately during tissue preparation it becomes deformed by changing the actual size of the sample, so this technique is not recommended to accurately determine the thickness of the epidermis.
  • OCT optical coherence tomography
  • LSM Ram Microscopy Laser
  • the OCT technique is capable of making vertical images in vivo, has a typical resolution in the range of 10 to 30 ⁇ and can be used to determine images in the millimeter range when there are contrasting agents that produce dispersion within the sample.
  • the LSM "Laser Sean Microscopy” is considered a high resolution technique ( ⁇ 1 ⁇ ) even when the depth of the image is limited to 200 ⁇ . This technique has the disadvantage that in order to accurately determine the thickness of the epidermis, a fluorescent agent must be injected.
  • WO / 2009/117485 "Optical method for determining morphological parameters and physiological properties of tissues”. The method uses reflectance measurements for various wavelengths, and using the radiative transport equation and numerical inversion methods, determines the parameters described above. Complex calculations are necessary to produce the numerical inversion for the determination of tissue properties.
  • WO / 2010/097218 "Method and apparatus for tissue imaging topography”. This solution consists of an optical device to generate images and measure the surface of the object, specular reflectance, and the structure of the lower layers. The device for creating the image eliminates specular reflection to allow a better obtaining of the surface structure. For its operation requires a CCD chip and a complex lens system.
  • This invention describes a method for determining the thickness of the skin layers using a spectrometer in diffuse reflectance mode for wavelengths between (700-2100 nm). It determines the attenuation of light based on the structure of the tissue and the chemical components of its constituents.
  • various analyzes involving multivariable calculation, and / or neural networks are required so that the thickness of the layers can be determined by mapping the relative intensities obtained from the various skin constituents.
  • a single calibration point is required by the device (achieved by OCT) to directly and immediately obtain the thickness of the epidermis.
  • the chemical components of their constituents are not taken into account to measure the thickness of the epidermis.
  • the proposed development discriminates any deviation that occurs in the measurement due to the effects induced by the variation of the thickness of the stratum corneum.
  • a low power laser is used, so it does not cause damage to the epidermis, an optical fiber and a simple photo-detector that together define the simplicity and low cost of the proposed technique.
  • Figure 1 Scheme of the device in use Figure 2.- Scheme of absorption and dispersion of light in the skin.
  • the present invention is a device that allows the thickness of the epidermis to be measured through the use of visible light, non-invasively and without affecting the integrity of the tissue.
  • the measurement on the skin is done directly and has a high response speed, so that the information can be given to the patient in situ.
  • the apparatus is formed by a light generating source (1), connected to at least one optical emission fiber (5), by means of which the light generated in (1) is directed towards the skin (2) affecting the surface of this, it also has at least one receiving optical fiber (6), which is responsible for receiving the light reflected or transmitted by the epidermis, said signal is conducted through (6) to reach a spectrometer (4), where the signal is processed to determine the thickness of the skin through a transmittance ratio.
  • the optical fibers are combined, both the emission (1) and the reception (5), and it is the one that comes into direct contact with the skin at the time of the test, its function It is to maintain the appropriate distance between the optical fibers (5 and 6) and insulate as much as possible the amount of light from the environment at the time of emission and capture of reflected light.
  • any type of fiber with high transmittance can be used.
  • the distance between fiber groups (5 and 6) can range from 200 ⁇ to ⁇
  • the group of fibers (5) are used for lighting, which are coupled to a visible light source (1) at one end and on the other is arranged at the tip (3), this source can be a halogen lamp , an LED or a diode laser, the latter preferably of red color and having a wavelength between 610-670 nm.
  • the end of the group of fibers (6) found in the tip (3) is used to capture the reflected light, and sent to be processed, its other end is connected to a spectrometer (4) where the signal is processed to be able to Determine the thickness of the skin.
  • the skin (2) where the measurement is to be performed must be in direct contact with the direct one with the tip (3) that contains the illumination fibers (5) and the reception fibers ( 6).
  • this invention holds that the measured value for transmittance is directly proportional to the thickness of the epidermis, so that any light source that includes this spectral range can be used.
  • a novel aspect of this development is that it does not require the use of the entire spectrum, only the range of visible light mentioned above.
  • the first group of lighting fibers (5) is connected to a light source (1), such as allogenous light whose spectrum is known; the second group of fibers or collection fibers (6) are connected to the spectrometer (4).
  • the end of the tip (3) in which both groups of fibers (5 and 6) are embedded are placed in direct contact with the skin (2) where the measurement is required to subsequently relate the measured transmittance to the thickness of the epidermis, as shown in the figural.
  • the device is activated and the visible light source (1) sends the beam through the group of emission fibers (5) affecting the skin , affected light (11), part of the light is absorbed and dispersed within the sample, as seen in Figure 2, crossing the corneal stratum (7), the epidermis (8), the dermis (9) and the fatty tissue (10), and part of the light is reflected in the upper layers and a percentage of light represents the transmitted light (12) itself that is captured by the group of receiving fibers (6), which is measured as transmittance in the Sample surface.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Dentistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Length Measuring Devices By Optical Means (AREA)

Abstract

L'invention concerne un dispositif pour déterminer l'épaisseur de l'épiderme, trouvant une application dans les sciences biologiques et faisant intervenir des mesures de transmittance in vivo, lequel dispositif comprend un arrangement de fibres optiques composé d'un groupe d'une ou de plusieurs fibres. Les deux groupes de fibres sont reliés à une extrémité à une distance caractéristique identique au diamètre de celles-ci. A l'autre extrémité, les groupes de fibres sont séparés, conçus de manière à ne pas tenir compte des variations d'épaisseur de la strate cornéenne de 400 μm, formant deux groupes indépendants. Les fibres peuvent présenter un diamètre compris entre 100 et 600 μm. Un groupe de fibres est utilisé pour l'éclairage, lequel groupe est couplé à une source de lumière visible, par exemple, une lampe halogène, une DEL ou un laser à diodes, lesquels sont, de préférence, de couleur rouge (610-670 nm), et l'autre groupe, destiné à la collecte, est utilisé pour mesurer le signal transmis qui est traité par un spectromètre.
PCT/MX2011/000061 2011-05-18 2011-05-18 Dispositif laser pour mesurer l'épaisseur de l'épiderme Ceased WO2012158007A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
PCT/MX2011/000061 WO2012158007A1 (fr) 2011-05-18 2011-05-18 Dispositif laser pour mesurer l'épaisseur de l'épiderme
MX2013013301A MX343805B (es) 2011-05-18 2011-05-18 Dispositivo láser para la medición de espesores de la epidermis,.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/MX2011/000061 WO2012158007A1 (fr) 2011-05-18 2011-05-18 Dispositif laser pour mesurer l'épaisseur de l'épiderme

Publications (1)

Publication Number Publication Date
WO2012158007A1 true WO2012158007A1 (fr) 2012-11-22

Family

ID=47177159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MX2011/000061 Ceased WO2012158007A1 (fr) 2011-05-18 2011-05-18 Dispositif laser pour mesurer l'épaisseur de l'épiderme

Country Status (1)

Country Link
WO (1) WO2012158007A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2728452A1 (fr) * 1994-12-22 1996-06-28 Dior Christian Parfums Dispositif pour l'analyse photoacoustique de peau in situ
US20010041829A1 (en) * 2000-01-12 2001-11-15 Suresh Thennadil Non-invasive method of determining skin thickness and characterizing layers of skin tissue in vivo
US20040142402A1 (en) * 2001-11-15 2004-07-22 Katsuhiko Maruo Method of measuring biological component concentration and apparatus therefor
KR20080061059A (ko) * 2006-12-28 2008-07-02 전자부품연구원 이미지센서를 이용한 피부두께 측정 장치 및 그 측정 방법
KR20080061058A (ko) * 2006-12-28 2008-07-02 전자부품연구원 리니어 이미지센서를 이용한 피부두께 측정 장치 및 그측정 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2728452A1 (fr) * 1994-12-22 1996-06-28 Dior Christian Parfums Dispositif pour l'analyse photoacoustique de peau in situ
US20010041829A1 (en) * 2000-01-12 2001-11-15 Suresh Thennadil Non-invasive method of determining skin thickness and characterizing layers of skin tissue in vivo
US20040142402A1 (en) * 2001-11-15 2004-07-22 Katsuhiko Maruo Method of measuring biological component concentration and apparatus therefor
KR20080061059A (ko) * 2006-12-28 2008-07-02 전자부품연구원 이미지센서를 이용한 피부두께 측정 장치 및 그 측정 방법
KR20080061058A (ko) * 2006-12-28 2008-07-02 전자부품연구원 리니어 이미지센서를 이용한 피부두께 측정 장치 및 그측정 방법

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