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WO2012033808A3 - Methods for determining the presence or zygosity of aad-12 soybean event 1606 - Google Patents

Methods for determining the presence or zygosity of aad-12 soybean event 1606 Download PDF

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Publication number
WO2012033808A3
WO2012033808A3 PCT/US2011/050644 US2011050644W WO2012033808A3 WO 2012033808 A3 WO2012033808 A3 WO 2012033808A3 US 2011050644 W US2011050644 W US 2011050644W WO 2012033808 A3 WO2012033808 A3 WO 2012033808A3
Authority
WO
WIPO (PCT)
Prior art keywords
assays
event
zygosity
aad
soybean
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/050644
Other languages
French (fr)
Other versions
WO2012033808A2 (en
Inventor
Ning Zhou
Steven N. Novak
Yunxing Cui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Corteva Agriscience LLC
Original Assignee
Dow AgroSciences LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow AgroSciences LLC filed Critical Dow AgroSciences LLC
Publication of WO2012033808A2 publication Critical patent/WO2012033808A2/en
Anticipated expiration legal-status Critical
Publication of WO2012033808A3 publication Critical patent/WO2012033808A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Provided herein are assays for detecting the presence of soybean event pDAB4472-1606 (Event 1606). The assays can be based on the DNA sequence of the recombinant construct, inserted into the soybean genome, and on the genomic sequences flanking the insertion site. The disclosure also relates in part to endpoint TaqMan PCR assays for the detection of Event 1606 that expresses AAD-12 in soybeans. Some assays are capable of high throughput zygosity analysis. These and other related procedures can be used to uniquely identify the zygosity of and breed soybean lines comprising the event disclosed. Kits and conditions useful in conducting these assays are also provided.
PCT/US2011/050644 2010-09-08 2011-09-07 Methods for determining the presence or zygosity of aad-12 soybean event 1606 Ceased WO2012033808A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38095210P 2010-09-08 2010-09-08
US61/380,952 2010-09-08

Publications (2)

Publication Number Publication Date
WO2012033808A2 WO2012033808A2 (en) 2012-03-15
WO2012033808A3 true WO2012033808A3 (en) 2014-03-20

Family

ID=45811138

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/050644 Ceased WO2012033808A2 (en) 2010-09-08 2011-09-07 Methods for determining the presence or zygosity of aad-12 soybean event 1606

Country Status (2)

Country Link
AR (1) AR082928A1 (en)
WO (1) WO2012033808A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UY34396A (en) * 2011-10-18 2013-05-31 Dow Agrosciences Llc MATERIALS AND METHODS FOR THE DETECTION OF THE ARYLOXIALCANOATE DIOXYGENASE GENE (AAD-12) IN THE PLANTS
CN119307649B (en) * 2024-12-02 2025-08-12 河北省农林科学院粮油作物研究所 InDel molecular marker primer for identifying soybean with high palmitic acid content and application thereof
CN120591459B (en) * 2025-08-05 2025-10-21 隆平生物技术(海南)有限公司 Transgenic soybean event LP689-1 and its detection method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060130175A1 (en) * 2002-10-29 2006-06-15 Ellis Daniel M Cot102 insecticidal cotton
WO2007053482A2 (en) * 2005-10-28 2007-05-10 Dow Agrosciences Llc Novel herbicide resistance genes
US20070271630A1 (en) * 2005-02-24 2007-11-22 Boukharov Andrey A Methods for genetic control of plant pest infestation and compositions thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060130175A1 (en) * 2002-10-29 2006-06-15 Ellis Daniel M Cot102 insecticidal cotton
US20070271630A1 (en) * 2005-02-24 2007-11-22 Boukharov Andrey A Methods for genetic control of plant pest infestation and compositions thereof
WO2007053482A2 (en) * 2005-10-28 2007-05-10 Dow Agrosciences Llc Novel herbicide resistance genes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK 27 February 2009 (2009-02-27), AINLEY ET AL.: "Anti-T Cell and Autoantigen Treatment of Autoimmune Disease.", retrieved from http://www.ncbi.nlm.nih.gov/nuccore/DM036188 accession no. M036188 *

Also Published As

Publication number Publication date
AR082928A1 (en) 2013-01-16
WO2012033808A2 (en) 2012-03-15

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