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WO2012031910A3 - Method for preparing alpha-ketopimelic acid by c1-elongation - Google Patents

Method for preparing alpha-ketopimelic acid by c1-elongation Download PDF

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Publication number
WO2012031910A3
WO2012031910A3 PCT/EP2011/064718 EP2011064718W WO2012031910A3 WO 2012031910 A3 WO2012031910 A3 WO 2012031910A3 EP 2011064718 W EP2011064718 W EP 2011064718W WO 2012031910 A3 WO2012031910 A3 WO 2012031910A3
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WO
WIPO (PCT)
Prior art keywords
homo
alpha
enzyme
acid
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2011/064718
Other languages
French (fr)
Other versions
WO2012031910A2 (en
Inventor
Axel Christoph Trefzer
Stefanus Cornelis Hendrikus Jozef Turk
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
Original Assignee
DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of WO2012031910A2 publication Critical patent/WO2012031910A2/en
Publication of WO2012031910A3 publication Critical patent/WO2012031910A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/50—Polycarboxylic acids having keto groups, e.g. 2-ketoglutaric acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01087—Homoisocitrate dehydrogenase (1.1.1.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01114—Methanogen homoaconitase (4.2.1.114)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst catalysing at least one of these conversions, wherein the heterologous biocatalyst comprises a. an NifV enzyme or another Aks enzyme having homo(n)citrate activity or a homologue thereof having homo(n)citrate activity; b. an AksD enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, c. an AksE enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, d. an AksF enzyme having homon- isocitrate dehydrogenase or a homologue thereof having homon-aconitase activity.
PCT/EP2011/064718 2010-09-10 2011-08-26 Method for preparing alpha-ketopimelic acid by c1-elongation Ceased WO2012031910A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10176270.6 2010-09-10
EP10176270 2010-09-10

Publications (2)

Publication Number Publication Date
WO2012031910A2 WO2012031910A2 (en) 2012-03-15
WO2012031910A3 true WO2012031910A3 (en) 2012-06-07

Family

ID=43530673

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/064718 Ceased WO2012031910A2 (en) 2010-09-10 2011-08-26 Method for preparing alpha-ketopimelic acid by c1-elongation

Country Status (2)

Country Link
TW (1) TW201217536A (en)
WO (1) WO2012031910A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103937841A (en) * 2014-05-13 2014-07-23 上海交通大学 Application of enoyl coenzyme A hydratase in adipic acid biosynthesis

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2720997A1 (en) 2011-06-17 2014-04-23 Invista Technologies S.à.r.l. Use of hydrolases to increase monomer content in waste stream
BR112013033672A2 (en) 2011-06-30 2017-03-14 Invista Tech Sarl method for converting a compound, substantially pure host cell culture and isolated cell
US9102960B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
US9102958B2 (en) 2011-12-16 2015-08-11 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals via CoA-dependent carbon chain elongation associated with carbon storage
EP2794897A2 (en) * 2011-12-21 2014-10-29 Invista Technologies S.a r.l. Bioconversion process for producing nylon-7, nylon-7,7 and polyesters
EP2931907A2 (en) 2012-12-14 2015-10-21 Invista Technologies S.A R.L. METHODS OF PRODUCING 7-CARBON CHEMICALS VIA CoA-DEPENDENT CARBON CHAIN ELONGATION ASSOCIATED WITH CARBON STORAGE
EP2938733A2 (en) 2012-12-31 2015-11-04 Invista North America S.a.r.l. Methods of producing 7-carbon chemicals via methyl-ester shielded carbon chain elongation
US9738911B2 (en) 2012-12-31 2017-08-22 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via pyruvate and succinate semialdehyde aldol condensation
US9617572B2 (en) 2012-12-31 2017-04-11 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via aromatic compounds
CN105026570A (en) 2012-12-31 2015-11-04 英威达技术有限责任公司 Methods of producing 7-carbon chemicals from long chain fatty acids via oxidative cleavage
CN105189770A (en) 2012-12-31 2015-12-23 英威达技术有限责任公司 Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis
WO2014105790A2 (en) 2012-12-31 2014-07-03 Invista North America S.A.R.L. Methods of producing 7-carbon chemicals via c1 carbon chain elongation associated with coenzyme b synthesis
US9580733B2 (en) 2012-12-31 2017-02-28 Invista North America S.A.R.L. Methods of producing 6-carbon chemicals via methyl-ester shielded carbon chain elongation
WO2015176026A1 (en) 2014-05-15 2015-11-19 Invista North America S.á.r.l. Methods of producing 6-carbon chemicals using 2,6-diaminopimelate as precursor to 2-aminopimelate
EP3155112A1 (en) 2014-06-16 2017-04-19 Invista Technologies S.à.r.l. Process for producing glutarate and glutaric acid methyl ester
CN106795530A (en) 2014-06-16 2017-05-31 英威达技术有限责任公司 Methods, reagents and cells for the biosynthesis of compounds
BR112016029365A2 (en) 2014-06-16 2017-10-31 Invista Tech Sarl methods, reagents and cells for compound biosynthesis
WO2015195707A1 (en) 2014-06-16 2015-12-23 Invista Technologies S.A.R.L. Methods, reagents and cells for biosynthesizing compounds
CN109337938B (en) * 2018-08-31 2021-05-07 河南师范大学 Method for producing 2-oxoadipic acid by fermenting and degrading paraffin with alkanobacterium

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008000632A1 (en) * 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
WO2009113855A2 (en) * 2008-03-11 2009-09-17 Dsm Ip Assets B.V. PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID
WO2010068944A2 (en) * 2008-12-12 2010-06-17 Celexion, Llc Biological synthesis of difunctional alkanes from carbohydrate feedstocks
WO2010104390A2 (en) * 2009-03-11 2010-09-16 Dsm Ip Assets B.V. Preparation of alpha-ketopimelic acid
WO2011031147A1 (en) * 2009-09-11 2011-03-17 Dsm Ip Assets B.V. Preparation of a compound comprising an amine group from an alpha-keto acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (en) 1986-03-17 1986-03-17 Novo Industri As PREPARATION OF PROTEINS
KR100516986B1 (en) 1997-02-19 2005-09-26 코닌클리즈케 디에스엠 엔.브이. Process for the preparation of caprolactam in the absence of catalysts by contacting 6-aminocaproic acid derivatives with superheated steam
US7402383B2 (en) 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
EP2194139A1 (en) 2004-01-19 2010-06-09 DSM IP Assets B.V. Biochemical synthesis of 6 amino caproic acid
DK1733040T3 (en) 2004-04-02 2010-11-22 Dsm Ip Assets Bv Filamentous fungal mutants with improved homologous recombination efficiency

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008000632A1 (en) * 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
WO2009113855A2 (en) * 2008-03-11 2009-09-17 Dsm Ip Assets B.V. PREPARATION OF 6-AMINOCAPROIC ACID FROM α-KETOPIMELIC ACID
WO2010068944A2 (en) * 2008-12-12 2010-06-17 Celexion, Llc Biological synthesis of difunctional alkanes from carbohydrate feedstocks
WO2010104390A2 (en) * 2009-03-11 2010-09-16 Dsm Ip Assets B.V. Preparation of alpha-ketopimelic acid
WO2011031147A1 (en) * 2009-09-11 2011-03-17 Dsm Ip Assets B.V. Preparation of a compound comprising an amine group from an alpha-keto acid

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [Online] 30 June 2007 (2007-06-30), "Methanococcus aeolicus Nankai-3, complete genome.", XP002639544, retrieved from EBI accession no. EMBL:CP000743 Database accession no. CP000743 *
DREVLAND RANDY M ET AL: "Methanogen Homoaconitase Catalyzes Both Hydrolyase Reactions in Coenzyme B Biosynthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 283, no. 43, 24 October 2008 (2008-10-24), pages 28888 - 28896, XP002570860, ISSN: 0021-9258, [retrieved on 20080902], DOI: 10.1074/JBC.M802159200 *
GRAHAM DAVID E ET AL: "Elucidation of methanogenic coenzyme biosyntheses: from spectroscopy to genomics", NATURAL PRODUCT REPORTS, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 19, no. 2, 1 April 2002 (2002-04-01), pages 133 - 147, XP002570861, ISSN: 0265-0568, DOI: 10.1039/B103714P *
HOWELL DAVID M ET AL: "alpha-Keto acid chain elongation reactions involved in the biosynthesis of coenzyme B (7-mercaptoheptanoyl threonine phosphate) in methanogenic archaea", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 37, no. 28, 14 July 1998 (1998-07-14), pages 10108 - 10117, XP002503245, ISSN: 0006-2960, DOI: 10.1021/BI980662P *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103937841A (en) * 2014-05-13 2014-07-23 上海交通大学 Application of enoyl coenzyme A hydratase in adipic acid biosynthesis
CN103937841B (en) * 2014-05-13 2015-10-28 上海交通大学 The application of enoyl-CoA hydratase in hexanodioic acid biosynthesizing

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WO2012031910A2 (en) 2012-03-15
TW201217536A (en) 2012-05-01

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