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WO2012003461A3 - Phloroglucinol synthases and methods of making and using the same - Google Patents

Phloroglucinol synthases and methods of making and using the same Download PDF

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Publication number
WO2012003461A3
WO2012003461A3 PCT/US2011/042822 US2011042822W WO2012003461A3 WO 2012003461 A3 WO2012003461 A3 WO 2012003461A3 US 2011042822 W US2011042822 W US 2011042822W WO 2012003461 A3 WO2012003461 A3 WO 2012003461A3
Authority
WO
WIPO (PCT)
Prior art keywords
phloroglucinol
methods
making
methyl
same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/042822
Other languages
French (fr)
Other versions
WO2012003461A2 (en
Inventor
John Frost
Craig Banotai
Betsy Lehner
Man Kit Lau
Justas Jancauskas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amyris Inc
Original Assignee
Amyris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Inc filed Critical Amyris Inc
Publication of WO2012003461A2 publication Critical patent/WO2012003461A2/en
Publication of WO2012003461A3 publication Critical patent/WO2012003461A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/22—Preparation of oxygen-containing organic compounds containing a hydroxy group aromatic
    • C—CHEMISTRY; METALLURGY
    • C06—EXPLOSIVES; MATCHES
    • C06B—EXPLOSIVES OR THERMIC COMPOSITIONS; MANUFACTURE THEREOF; USE OF SINGLE SUBSTANCES AS EXPLOSIVES
    • C06B25/00—Compositions containing a nitrated organic compound
    • C06B25/04—Compositions containing a nitrated organic compound the nitrated compound being an aromatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention provides nucleic acids, amino acids, cells, and related methods for the biosynthetic production of phloroglucinol. In various embodiments, the invention includes an isolated or recombinant phloroglucinol synthase, which can be used in conjunction with an enzyme system for the biosynthetic production of phloroglucinol, mono-O-methyl phloroglucinol, di-O-methyl phloroglucinol and tri-O-methyl phloroglucinol. The synthesis can be from a renewable carbon source. The phloroglucinol can be used in subsequent reactions.
PCT/US2011/042822 2010-07-02 2011-07-01 Phloroglucinol synthases and methods of making and using the same Ceased WO2012003461A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39889710P 2010-07-02 2010-07-02
US61/398,897 2010-07-02

Publications (2)

Publication Number Publication Date
WO2012003461A2 WO2012003461A2 (en) 2012-01-05
WO2012003461A3 true WO2012003461A3 (en) 2012-05-03

Family

ID=44561233

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/042822 Ceased WO2012003461A2 (en) 2010-07-02 2011-07-01 Phloroglucinol synthases and methods of making and using the same

Country Status (1)

Country Link
WO (1) WO2012003461A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399142A (en) * 2015-07-30 2017-02-15 北京大学 Seaweed epiphytic pseudomonas capable of producing phloroglucinol and artificial culture method of seaweed epiphytic pseudomonas
CN105734003B (en) * 2015-12-20 2019-09-13 寿光富康制药有限公司 A kind of seaweed fungal component and its method for preparing phloroglucin
FR3068368A1 (en) 2017-06-30 2019-01-04 Compagnie Generale Des Etablissements Michelin USE OF TYPE III POLYKETIDE SYNTHASES OF BACTERIA AS PHLOROGLUCINOL SYNTHASES
FR3068367A1 (en) * 2017-06-30 2019-01-04 Compagnie Generale Des Etablissements Michelin USE OF TYPE III POLYKETIDE SYNTHASES AS PHLOROGLUCINOL SYNTHASES
CN109456927A (en) * 2018-11-14 2019-03-12 中国科学院青岛生物能源与过程研究所 The recombinant bacterium and its construction method of a kind of high yield 2,4- diacetyl phloroglucin and application
FR3092339B1 (en) * 2019-02-05 2024-04-12 Michelin & Cie CELLS, PARTICULARLY YEAST, RESISTANT TO PHLOROGLUCINOL

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006044290A2 (en) * 2004-10-12 2006-04-27 Board Of Trustees Of Michigan State University Biosynthesis of philoroglucinol and preparation of 1,3-dihydroxybenzene therefrom

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5350576A (en) 1991-09-13 1994-09-27 Mycogen Corporation Bacillus thuringiensis isolates for controlling acarides
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US6194167B1 (en) 1997-02-18 2001-02-27 Washington State University Research Foundation ω-3 fatty acid desaturase
US6774107B1 (en) 1999-03-18 2004-08-10 Merck Patent Gmbh Protein for blocking platelet adhesion
US20030031670A1 (en) 1999-11-08 2003-02-13 Jack R. Wands Diagnosis and treatment of malignant neoplasms
EP1130098A3 (en) 2000-02-29 2003-09-10 Pfizer Products Inc. Mammalian osteoregulins
US6555353B2 (en) 2000-09-01 2003-04-29 E. I. Du Pont De Nemours And Company Methanotrophic carbon metabolism pathway genes and enzymes
US7531593B2 (en) 2006-10-31 2009-05-12 E.I. Du Pont De Nemours And Company Thermoplastic elastomer blend composition

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006044290A2 (en) * 2004-10-12 2006-04-27 Board Of Trustees Of Michigan State University Biosynthesis of philoroglucinol and preparation of 1,3-dihydroxybenzene therefrom

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ACHKAR JIHANE ET AL: "Biosynthesis of phloroglucinol", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC; US, vol. 127, no. 15, 26 March 2005 (2005-03-26), pages 5332 - 5333, XP002397679, ISSN: 0002-7863, DOI: 10.1021/JA042340G *
DATABASE EMBL [Online] 6 November 2009 (2009-11-06), "Vibrio sp. RC586 VOA.Contig114, whole genome shotgun sequence.", XP002659412, retrieved from EBI accession no. EMBL:ADBD01000013 Database accession no. ADBD01000013 *
DATABASE UniProt [Online] 15 December 2009 (2009-12-15), "SubName: Full=PrpF protein involved in 2-methylcitrate cycle;", XP002659413, retrieved from EBI accession no. UNIPROT:D0IK39 Database accession no. D0IK39 *
WU S ET AL: "The key role of phloroglucinol O-methyltransferase in the biosynthesis of Rosa chinensis volatile 1,3,5-trimethoxybenzene", PLANT PHYSIOLOGY MAY 2004 AMERICAN SOCIETY OF PLANT BIOLOGISTS US, vol. 135, no. 1, May 2004 (2004-05-01), pages 95 - 102, XP002659414, DOI: DOI:10.1104/PP.103.037051 *

Also Published As

Publication number Publication date
WO2012003461A2 (en) 2012-01-05

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