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WO2012087038A3 - 신규 ｏ-아세틸호모세린 설피드릴라아제 또는 이의 변이체 및 이를 이용한 메치오닌 전환 방법 - Google Patents

신규 ｏ-아세틸호모세린 설피드릴라아제 또는 이의 변이체 및 이를 이용한 메치오닌 전환 방법 Download PDF

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Publication number
WO2012087038A3
WO2012087038A3 PCT/KR2011/009965 KR2011009965W WO2012087038A3 WO 2012087038 A3 WO2012087038 A3 WO 2012087038A3 KR 2011009965 W KR2011009965 W KR 2011009965W WO 2012087038 A3 WO2012087038 A3 WO 2012087038A3
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WO
WIPO (PCT)
Prior art keywords
methionine
acetic acid
variant
novel
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2011/009965
Other languages
English (en)
French (fr)
Other versions
WO2012087038A2 (ko
Inventor
김소영
신용욱
서창일
손성광
허인경
이한진
김주은
김현아
배지연
나광호
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CJ CheilJedang Corp
Original Assignee
CJ CheilJedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CJ CheilJedang Corp filed Critical CJ CheilJedang Corp
Priority to CN201180065568.6A priority Critical patent/CN103328498B/zh
Priority to JP2013546020A priority patent/JP5907993B2/ja
Priority to RU2013133636/10A priority patent/RU2573928C2/ru
Priority to DK11850058.6T priority patent/DK2657249T3/en
Priority to EP11850058.6A priority patent/EP2657249B1/en
Priority to BR112013015992-8A priority patent/BR112013015992B1/pt
Priority to US13/997,054 priority patent/US9080155B2/en
Priority to ES11850058.6T priority patent/ES2627792T3/es
Publication of WO2012087038A2 publication Critical patent/WO2012087038A2/ko
Publication of WO2012087038A3 publication Critical patent/WO2012087038A3/ko
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/54—Acetic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01049—O-acetylhomoserine aminocarboxypropyltransferase (2.5.1.49)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 O-아세틸호모세린 설피드릴라아제(O-acetylhomoserine sulfhydrylase)의 활성을 가지는 신규 단백질, 이의 변이형 단백질, 이들을 암호화하는 폴리뉴클레오티드, 상기 폴리뉴클레오티드를 포함하는 재조합 벡터, 상기 재조합 벡터로 형질전환된 미생물, 및 상기 단백질을 이용하여 메치오닌 또는 아세트산을 생산하는 방법에 관한 것이다. 본 발명의 생산방법은 기존의 방법보다 높은 전환율 및 반응시간 단축을 통하여 경제적으로 L-메치오닌 및 아세트산을 생산할 수 있는 이점이 있으며, 상기 변이형 단백질을 이용하는 경우 효소 파쇄액의 투입량을 최소화하여 L-메치오닌 및 아세트산을 고수율로 용이하게 생산할 수 있다.
PCT/KR2011/009965 2010-12-21 2011-12-21 신규 ｏ-아세틸호모세린 설피드릴라아제 또는 이의 변이체 및 이를 이용한 메치오닌 전환 방법 Ceased WO2012087038A2 (ko)

Priority Applications (8)

Application Number Priority Date Filing Date Title
CN201180065568.6A CN103328498B (zh) 2010-12-21 2011-12-21 新的o-乙酰高丝氨酸硫化氢解酶或其突变体蛋白，和使用其转化成甲硫氨酸的方法
JP2013546020A JP5907993B2 (ja) 2010-12-21 2011-12-21 新規ｏ−アセチルホモセリンスルフヒドリラーゼまたはその変異タンパク質およびこれを用いたメチオニン転換方法
RU2013133636/10A RU2573928C2 (ru) 2010-12-21 2011-12-21 Новая о-ацетилгомосерин-сульфгидрилаза или ее мутантный белок и способ преобразования в метионин с использованием таковой
DK11850058.6T DK2657249T3 (en) 2010-12-21 2011-12-21 New O-acetylhomoserine sulfhydrylase or variant thereof and method for transformation of methionine using the same
EP11850058.6A EP2657249B1 (en) 2010-12-21 2011-12-21 Novel o-acetylhomoserine sulfhydrylase or variant thereof, and method for transforming methionine using same
BR112013015992-8A BR112013015992B1 (pt) 2010-12-21 2011-12-21 Proteína possuindo atividade de sulfidrilase de o-acetilhomoserina, polinucleotídeo, vetor recombinante, microrganismo e método para produzir metionina ou ácido acético
US13/997,054 US9080155B2 (en) 2010-12-21 2011-12-21 O-acetylhomoserine sulfhydrylase or mutant protein thereof, and method for converting to methionine using the same
ES11850058.6T ES2627792T3 (es) 2010-12-21 2011-12-21 O-acetilhomoserina sulfhidrilasa novedosa o variante de la misma y procedimiento para transformar la metionina usando la misma

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100131954A KR101250651B1 (ko) 2010-12-21 2010-12-21 신규 ｏ-아세틸호모세린 설피드릴라제 또는 변이체 및 이를 이용한 메치오닌 전환 방법
KR10-2010-0131954 2010-12-21

Publications (2)

Publication Number Publication Date
WO2012087038A2 WO2012087038A2 (ko) 2012-06-28
WO2012087038A3 true WO2012087038A3 (ko) 2012-08-23

Family

ID=46314641

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/009965 Ceased WO2012087038A2 (ko) 2010-12-21 2011-12-21 신규 ｏ-아세틸호모세린 설피드릴라아제 또는 이의 변이체 및 이를 이용한 메치오닌 전환 방법

Country Status (11)

Country Link
US (1) US9080155B2 (ko)
EP (1) EP2657249B1 (ko)
JP (1) JP5907993B2 (ko)
KR (1) KR101250651B1 (ko)
CN (1) CN103328498B (ko)
BR (1) BR112013015992B1 (ko)
DK (1) DK2657249T3 (ko)
ES (1) ES2627792T3 (ko)
PL (1) PL2657249T3 (ko)
RU (1) RU2573928C2 (ko)
WO (1) WO2012087038A2 (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101622491B1 (ko) * 2011-09-02 2016-05-18 아르끄마 프랑스 L-메티오닌의 제조 방법
KR101859276B1 (ko) 2015-06-04 2018-05-18 씨제이제일제당 (주) O-아세틸-호모세린을 생산하는 미생물 및 이를 이용하여 o-아세틸-호모세린을 생산하는 방법
WO2017009047A1 (en) * 2015-07-13 2017-01-19 Evonik Degussa Gmbh Method for producing l-methionine
US10378031B2 (en) 2015-10-13 2019-08-13 Cj Cheiljedang Corporation O-acetylhomoserine sulfhydrylase variant and method for producing L-methionine using same
KR102016050B1 (ko) 2018-03-20 2019-08-29 씨제이제일제당 주식회사 신규한 프로모터 및 이의 용도
KR102377500B1 (ko) * 2019-10-28 2022-03-23 씨제이제일제당 주식회사 외래 metZ 유전자에 의해 코딩되는 단백질이 도입된 L-메티오닌 생산 미생물 및 이를 이용한 L-메티오닌 생산방법
WO2024162751A1 (en) * 2023-01-31 2024-08-08 Cj Cheiljedang Corporation O-acylhomoserine sulfhydrylase variant and use thereof
CN120843473A (zh) * 2025-09-25 2025-10-28 山东天力药业有限公司 一种o-乙酰高丝氨酸硫化氢解酶突变体、表达该突变体的重组工程菌及其应用

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WO2004108894A2 (en) * 2003-05-30 2004-12-16 Microbia, Inc. Methods and compositions for amino acid production
KR20080011132A (ko) * 2006-07-28 2008-01-31 씨제이제일제당 (주) Ｌ-메치오닌 전구체 생산 균주 및 상기 ｌ-메치오닌전구체로부터의 ｌ-메치오닌 및 유기산의 생산방법
KR20090106365A (ko) * 2008-04-04 2009-10-08 씨제이제일제당 (주) Ｌ-메치오닌 전구체 생산 균주 및 이를 이용한 ｌ-메치오닌 전구체의 생산 방법
US7611873B1 (en) * 1998-11-17 2009-11-03 Ajinomoto Co., Inc. Method for producing L-methionine by fermentation

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DE10109690A1 (de) * 2000-09-02 2002-03-14 Degussa Neue für das metY-Gen kodierende Nukleotidsequenzen
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
CA2615315C (en) * 2005-07-18 2015-10-06 Basf Aktiengesellschaft Use of dimethyl disulfide for methionine production in microorganisms
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WO2004108894A2 (en) * 2003-05-30 2004-12-16 Microbia, Inc. Methods and compositions for amino acid production
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Also Published As

Publication number Publication date
KR20120070411A (ko) 2012-06-29
WO2012087038A2 (ko) 2012-06-28
CN103328498B (zh) 2015-11-25
US20130273614A1 (en) 2013-10-17
RU2573928C2 (ru) 2016-01-27
JP5907993B2 (ja) 2016-04-26
US9080155B2 (en) 2015-07-14
CN103328498A (zh) 2013-09-25
KR101250651B1 (ko) 2013-04-03
RU2013133636A (ru) 2015-01-27
ES2627792T3 (es) 2017-07-31
BR112013015992A2 (pt) 2020-07-07
EP2657249A4 (en) 2014-10-01
EP2657249A2 (en) 2013-10-30
DK2657249T3 (en) 2017-06-06
PL2657249T3 (pl) 2017-08-31
JP2014506129A (ja) 2014-03-13
BR112013015992B1 (pt) 2022-01-25
EP2657249B1 (en) 2017-03-15

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