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WO2011115975A3 - Use of achromopeptidase for lysis at room temperature - Google Patents

Use of achromopeptidase for lysis at room temperature Download PDF

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Publication number
WO2011115975A3
WO2011115975A3 PCT/US2011/028494 US2011028494W WO2011115975A3 WO 2011115975 A3 WO2011115975 A3 WO 2011115975A3 US 2011028494 W US2011028494 W US 2011028494W WO 2011115975 A3 WO2011115975 A3 WO 2011115975A3
Authority
WO
WIPO (PCT)
Prior art keywords
achromopeptidase
sample
room temperature
gram
absence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2011/028494
Other languages
French (fr)
Other versions
WO2011115975A2 (en
Inventor
Danielle Hilligoss
Lisa M. Keller
Samah Ramadan
Jamie Coady
Tobin J. Hellyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Priority to CN2011800203890A priority Critical patent/CN102869773A/en
Priority to BR112012023875A priority patent/BR112012023875A2/en
Priority to JP2013500151A priority patent/JP2013521804A/en
Priority to EP11756845.1A priority patent/EP2547767A4/en
Priority to AU2011227452A priority patent/AU2011227452B2/en
Priority to CA2793144A priority patent/CA2793144A1/en
Publication of WO2011115975A2 publication Critical patent/WO2011115975A2/en
Publication of WO2011115975A3 publication Critical patent/WO2011115975A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A process for detecting the presence or absence of gram-positive bacteria in a biological sample. The biological sample can be obtained from any mammal and contains, at a minimum, cellular components. The sample is combined with an enzymatic lysing agent such as achromopeptidase, and lysed at room temperature. Ferric oxide is then added to the sample containing achromopeptidase. A magnetic field is applied to the sample and nucleic acids are extracted from the cellular components. Target nucleic acids, if present, are amplified using techniques such as Polymerase Chain Reaction (PCR) and then used to detect the presence or absence of gram-positive bacteria. Staphylococcus aureus and Streptococcus agalactiae are examples of target bacteria detected by the methods of the present invention.
PCT/US2011/028494 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature Ceased WO2011115975A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN2011800203890A CN102869773A (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature
BR112012023875A BR112012023875A2 (en) 2010-03-16 2011-03-15 use of acromopeptidase for lysis at room temperature
JP2013500151A JP2013521804A (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature
EP11756845.1A EP2547767A4 (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature
AU2011227452A AU2011227452B2 (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature
CA2793144A CA2793144A1 (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31431810P 2010-03-16 2010-03-16
US61/314,318 2010-03-16

Publications (2)

Publication Number Publication Date
WO2011115975A2 WO2011115975A2 (en) 2011-09-22
WO2011115975A3 true WO2011115975A3 (en) 2012-01-19

Family

ID=44649786

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/028494 Ceased WO2011115975A2 (en) 2010-03-16 2011-03-15 Use of achromopeptidase for lysis at room temperature

Country Status (8)

Country Link
US (1) US20110275090A1 (en)
EP (1) EP2547767A4 (en)
JP (1) JP2013521804A (en)
CN (1) CN102869773A (en)
AU (1) AU2011227452B2 (en)
BR (1) BR112012023875A2 (en)
CA (1) CA2793144A1 (en)
WO (1) WO2011115975A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8597884B2 (en) 2011-03-09 2013-12-03 Becton, Dickinson And Company Process controls for molecular assay
US9528987B2 (en) 2011-06-23 2016-12-27 University Of Washington Reagent patterning in capillarity-based analyzers and associated systems and methods
WO2013117968A1 (en) 2012-02-10 2013-08-15 Reametrix Inc. Methods for lysing bacteria from sample and isolating cellular components therefrom, and kits therefor
WO2014116756A1 (en) 2013-01-22 2014-07-31 University Of Washington Through Its Center For Commercialization Sequential delivery of fluid volumes and associated devices, systems and methods
EP3320966B1 (en) 2016-11-14 2022-02-16 Airbus Operations GmbH Autoclave and method for welding thermoplastic composite parts
WO2019055331A1 (en) * 2017-09-13 2019-03-21 Becton, Dickinson And Company Methods and compositions for extracting nucleic acids using ferric oxide particles
WO2021217173A1 (en) * 2020-04-21 2021-10-28 Siemens Healthcare Diagnostics Inc. Clinical specimen collection swabs for molecular diagnostic applications and methods of production and use thereof
CN118834934B (en) * 2024-08-19 2025-05-16 希莱乐检(郑州)生物科技有限公司 Lysis solution for releasing nucleic acid of Mycobacterium tuberculosis and its application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
US5508164A (en) * 1990-10-29 1996-04-16 Dekalb Genetics Corporation Isolation of biological materials using magnetic particles
US6433160B1 (en) * 1998-10-30 2002-08-13 Becton, Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
US20040002594A1 (en) * 2002-06-27 2004-01-01 John Krupey Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8414273D0 (en) * 1984-06-05 1984-07-11 Oxoid Ltd Identifying streptococcal grouping
JP2589729B2 (en) * 1988-01-30 1997-03-12 極東製薬工業株式会社 Bacterial identification method using chromosomal DNA and kit for identification
JP3172917B2 (en) * 1999-07-23 2001-06-04 北海道 Bacteria detection method
US7595176B2 (en) * 2003-05-30 2009-09-29 Georgia Tech Research Corporation Methods and reagents for quantitative analysis of Dehalococcoides species
JP4621919B2 (en) * 2003-12-26 2011-02-02 プリマハム株式会社 Multiplex detection method for microorganisms
WO2006028601A2 (en) * 2004-07-26 2006-03-16 Nanosphere, Inc. Method for distinguishing methicillin resistant s. aureus from methicillin sensitive s. aureus in a mixed culture
CA2615984C (en) * 2005-07-21 2013-04-23 Morinaga Milk Industry Co., Ltd. Method for detection of microorganism and kit for detection of microorganism
EP2095125A2 (en) * 2006-11-22 2009-09-02 3M Innovative Properties Company Systems and methods for preparing and analyzing samples
JP2008148570A (en) * 2006-12-14 2008-07-03 Hitachi Ltd Microorganism detection system
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090274577A1 (en) * 2008-05-05 2009-11-05 B-K Medical Aps Sterilization by treatment with reductant and oxidant

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5508164A (en) * 1990-10-29 1996-04-16 Dekalb Genetics Corporation Isolation of biological materials using magnetic particles
US5185242A (en) * 1991-06-24 1993-02-09 Becton Dickinson And Company Method for lysing mycobacteria using achromopeptidase
US6433160B1 (en) * 1998-10-30 2002-08-13 Becton, Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
US20040002594A1 (en) * 2002-06-27 2004-01-01 John Krupey Removal of extraneous substances from biological fluids containing nucleic acids and the recovery of nucleic acids

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PAULE ET AL.: "Direct detection of Staphylococcus aureus from adult and neonate nasal swab specimens using real-time polymerase chain reaction.", J MOL DIAGN., vol. 6, no. 3, August 2004 (2004-08-01), pages 191 - 196, XP009045081 *
See also references of EP2547767A4 *
SEKAR ET AL.: "An improved protocol for quantification of freshwater Actinobacteria by fluorescence in situ hybridization.", APPL ENVIRON MICROBIOL., vol. 69, no. 5, May 2003 (2003-05-01), pages 2928 - 2935, XP055087056, DOI: doi:10.1128/AEM.69.5.2928-2935.2003 *

Also Published As

Publication number Publication date
US20110275090A1 (en) 2011-11-10
WO2011115975A2 (en) 2011-09-22
AU2011227452B2 (en) 2015-04-09
EP2547767A4 (en) 2013-12-25
JP2013521804A (en) 2013-06-13
AU2011227452A1 (en) 2012-10-04
BR112012023875A2 (en) 2015-09-22
CA2793144A1 (en) 2011-09-22
EP2547767A2 (en) 2013-01-23
CN102869773A (en) 2013-01-09

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