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WO2011009184A1 - Utilisation du mesquite pour la production d'un polymère polysaccharidique à base aqueuse du type gomme xanthique - Google Patents

Utilisation du mesquite pour la production d'un polymère polysaccharidique à base aqueuse du type gomme xanthique Download PDF

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Publication number
WO2011009184A1
WO2011009184A1 PCT/BR2010/000247 BR2010000247W WO2011009184A1 WO 2011009184 A1 WO2011009184 A1 WO 2011009184A1 BR 2010000247 W BR2010000247 W BR 2010000247W WO 2011009184 A1 WO2011009184 A1 WO 2011009184A1
Authority
WO
WIPO (PCT)
Prior art keywords
xanthan gum
production
mesquite
process according
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BR2010/000247
Other languages
English (en)
Portuguese (pt)
Inventor
Edson Quirino Buzanelli
Marino Tadeu Fabi
Jose Fernando Barreto Da Silva
Lucio Jose Sobral Rocha
Luca Pessoa Buzanelli
Alessander Acacio Ferro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Servico Nacional De Aprendizagem Industrial- Senai/dr-Ba
Quantas Biotecnologia SA
Original Assignee
Servico Nacional De Aprendizagem Industrial- Senai/dr-Ba
Quantas Biotecnologia SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Servico Nacional De Aprendizagem Industrial- Senai/dr-Ba, Quantas Biotecnologia SA filed Critical Servico Nacional De Aprendizagem Industrial- Senai/dr-Ba
Publication of WO2011009184A1 publication Critical patent/WO2011009184A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/06—Xanthan, i.e. Xanthomonas-type heteropolysaccharides

Definitions

  • the present invention relates to the use of the Algaroba vegetable (Prosopis julif ⁇ ora) as raw material for the industrial process of producing xanthan gum-type polysaccharide.
  • Xafitana gum is produced by the action of bacteria of the species Xanthomonas campestr ⁇ s or other Xanthomonas species undergoing aerobic fermentation from a carbon source.
  • Xanthan Gum Biopolymer is usable in a wide range of food, cosmetic, pharmaceutical, textile, explosive and petroleum industries, exhibiting excellent viscosity characteristics and having a significant emulsifying stabilizer effect as well as excellent salt resistance and high change resistance. as well as stability to enzymatic action which makes the use of Xanthan Gum as an important additive in considerable plurality of processes and products increasing.
  • This biopolymer has a molecular mass in the range of 0.5 to 12 million daltons and consists molecularly of a carbohydrate chain in which 2: 2: 1 glucose, mannose and glucuronic acid are arranged in a main chain consisting of D-bonds.
  • glucose-D-glucose (1-4) and in which every three glucose units there is a branched chain consisting of three carbohydrates being the one linked to the glucosidic chain, one acetylated mannose, the central carbohydrate being a glucuronic acid and the third carbohydrate.
  • a mannose containing 1,3-acetal linkage with pyruvic acid a mannose containing 1,3-acetal linkage with pyruvic acid.
  • Xanthan gum is produced by fermentation of carbohydrate-based carbon source generally glucose, sucrose, starch and others in a suitable culture medium where mineral nitrogen in the form of ammonium or oforganic nitrogen cations as well as potassium, magnesium, and other ions in smaller will concentrate, give conditions for the fermentative process to proceed following a known kinetics.
  • carbohydrate-based carbon source generally glucose, sucrose, starch and others
  • suitable culture medium where mineral nitrogen in the form of ammonium or oforganic nitrogen cations as well as potassium, magnesium, and other ions in smaller will concentrate, give conditions for the fermentative process to proceed following a known kinetics.
  • the usual procedure for performing fermentation involves two different steps. Initially, the microorganism to be used is subjected to suitable small-scale cultivation in order to obtain a relatively high concentration of bacteria corresponding to this phase called germination or growth. At this stage, the bacteria
  • the material resulting from this first stage having a relatively high concentration, is inoculated in the fermentation reactor where, in fermentation medium, said production, the development of the biopolymer is consolidated.
  • Raw materials for obtaining xanthan gum are usually carbohydrates such as glucose, dextrose and sucrose. Because sucrose and glucose are human foods, there is interest in using an alternative carbohydrate source whose use does not influence the food balance.
  • the algarobeira is a widespread, drought-resistant and widespread legume originating in Central and South America and even India, and it grows well in arid climates acting as a reforestation plant and fodder being a very well-developed xerophyte. in northeastern Brazil and even uncontrolled and aggressive being even competitive with the native flora.
  • the algarobeira is excellent for feeding the cattle with its foliage and seedlings and is also a source of excellent wood.
  • the resistance of this plant to desert climates is due to its ability to absorb air moisture which is fixed by condensation and stored until its roots are quite long.
  • Proper bacterial fermentation, the idea is to use mesquite and its bran directly in the production of xanthan gum.
  • This invention relates to the use of the Algaroba vegetable (Prosopis julif ⁇ ora) as a raw material for the industrial process of producing xanthan gum polysaccharide, bsise water.
  • the present invention relates to the use of the Algaroba (Prosopis julif ⁇ ora) vegetable pod meal to obtain the aqueous soluble biopolymer of the xanthan gum type.
  • Algaroba Prosopis julif ⁇ ora
  • Algaroba extract as a carbon source is then used as a developmental medium in the production phase to obtain aqueous soluble polysaccharide of the xanthan gum type.
  • Algaroba is the Prosopis julif ⁇ ora vegetable, a legume of enormous resistance to arid soils that characterizes the semi-arid and caatinga and that grows with great vigor in northeastern Brazil.
  • mesquite meal as a direct source for obtaining a sucrose-rich aqueous extract that is directly used as a source of carbon and the same culture medium for the production of xanthan gum biopolymer is an innovative alternative as it allows considerable gains.
  • mesquite meal as a direct source for obtaining a sucrose-rich aqueous extract that is directly used as a source of carbon and the same culture medium for the production of xanthan gum biopolymer is an innovative alternative as it allows considerable gains.
  • the carbon source and the cation and anion rich development and production medium required for the bacterium responsible for exopolymer production are already contained in this respective extract.
  • galactomannan In the endosclerosis, the predominant polysaccharide is galactomannan, consisting basically of 46.28% mannose and 33.97% galactose and which this patent highlights.
  • composition of the pods is as follows without regard to the saccharide content as indicated above.
  • Neutral Fiber Detergent 296.0 310.5 Fiber Acid Detergent 193.2 217.5 Cellulose 154.6 131.3 Lignin 38.6 86.2
  • Xanthomonas campestris cells are grown in mesquite extract medium with growth kinetics suitable for this medium.
  • the raw material is mesquite meal of the type supplied by specialized companies. This bran is extracted with water and the aqueous extract and used directly in the production of xanthan gum-soluble aqueous biopolymer.
  • composition of an extract of this bran is high in carbohydrates; at 50%, at 25% protein, 5% glucides, as well as the elements and microelements which highlight potassium, calcium, magnesium, iron, manganese 3 phosphate.
  • Meal meal is received from the plantation and production centers and properly stored with care for its maintenance and protected for its physical conservation and the development of organisms and microorganisms that may damage or compromise it.
  • the suspension is filtered through a 350 mesh filter and the material obtained is used in two different ways.
  • This aqueous extract has a 56% sucrose content as measured by HPLC. It should be noted that this process relates to obtaining and using the aqueous extract of the pod content in the case of bran form of that pod which is not restricted to that same form, and it may be an alternative to compress the pod into water without her previously dried i
  • 500ml of the solution obtained according to the previous item were sterilized at 125 ° C and to it were added 100ml of a solution of growth medium YM in which the microorganisms Xanthomonas campestris developed for 55 hours, according to the state of the art.
  • YM culture medium is, as known, composed of yeast, malt and nitrogen source in the peptone case.
  • the reference presents the preparation of this culture medium.
  • Such culture medium after sterilization at 125 ° C, was inoculated with the equivalent of 1 ml of previously developed solution and frozen in Ependorf of Xanthomonas campestris. There is no restriction on the Xanthomonas campestris strain to be used. After 55 hours of growth when the kinetics of this bacterial growth reaches its lag phase, this culture medium solution containing sufficiently grown microorganisms is added as indicated to 500 ml of the medium consisting of the mesquite bran aqueous extract.
  • the development of the biopolymer is continued under orbital shaker shaking at 27 ° C. After 72 hours, when the medium reaches viscosity and appearance indicating the presence of the biopolymer in adequate concentration, the medium is treated with iscpropanol and the xanthan gum is precipitated and, after washing, is dried and suitably characterized by chemical, chromatographic and spectroscopic techniques. .
  • aqueous solution resulting from the extraction of mesquite meal as growth medium of the Xanthomonas campestris microorganism and production medium of the xanthan gum biopolymer.
  • 500 ml of the solution obtained according to the previous item were sterilized at 125 ° C and to it is added the equivalent to an Ependorf corresponding to 1 ml of solution and culture medium with preserved Xanthomonas campestr ⁇ s cells, as described above. d'art of the production of the xanthan gum biopolymer.
  • the mesquite pod bran extract by such use, dispenses the use of raw sugar itself and dispenses the inputs that correspond to the usual growth and production means of xanthum gum.
  • the extract under consideration is the culture medium for fermentation with Xanthomonas campestr ⁇ s microorganisms resulting in xanthan gum produced entirely from mesquite extract replacing the usual sources of sucrose, glucose or dextrose monohydrate disaccharides. and also dispensing the culture medium components normally used for the production of such biopolymer.
  • aqueous extract of the mesquite pod already contains not only an adequate and high carbohydrate content, but also proteinaceous substances as well as mineral micronutrient contents which allow considerable simplification in the fermentation process, eliminating the necessary means.
  • Gum growth and production Xanthan being the fermentative procedure performed in medium of mesquite extract.

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé pratique consistant à utiliser le mesquite pour la production d'un biopolymère aqueux-soluble du type gomme xanthique, et permettant de remplacer le milieu de production ou le milieu de croissance, voire les deux, utilisés dans la technique actuelle de production de gomme xanthique, d'où l'obtention d'un procédé très avantageux d'un point de vue économique par rapport aux procédés classiques de production de gomme xanthique.
PCT/BR2010/000247 2009-07-24 2010-07-22 Utilisation du mesquite pour la production d'un polymère polysaccharidique à base aqueuse du type gomme xanthique Ceased WO2011009184A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI0902629-0 2009-07-24
BRPI0902629 BRPI0902629A2 (pt) 2009-07-24 2009-07-24 utilização de algaroba para a produção de polìmero polissacarìdeo base água do tipo goma xantana

Publications (1)

Publication Number Publication Date
WO2011009184A1 true WO2011009184A1 (fr) 2011-01-27

Family

ID=43498676

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2010/000247 Ceased WO2011009184A1 (fr) 2009-07-24 2010-07-22 Utilisation du mesquite pour la production d'un polymère polysaccharidique à base aqueuse du type gomme xanthique

Country Status (2)

Country Link
BR (1) BRPI0902629A2 (fr)
WO (1) WO2011009184A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8601641A (pt) * 1986-04-11 1987-11-03 Petroleo Brasileiro Sa Processo de obtencao de galactomanana e composicao para fluido de fraturamento de formacoes
WO1995005476A1 (fr) * 1993-08-19 1995-02-23 Nera-Associação Empresarial Da Região Do Algarve Procede de production de xanthane a l'aide d'acides organiques non metabolisables
WO2001018226A1 (fr) * 1999-09-07 2001-03-15 Rhodia Chimie Procede de production d'exopolysaccharides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR8601641A (pt) * 1986-04-11 1987-11-03 Petroleo Brasileiro Sa Processo de obtencao de galactomanana e composicao para fluido de fraturamento de formacoes
WO1995005476A1 (fr) * 1993-08-19 1995-02-23 Nera-Associação Empresarial Da Região Do Algarve Procede de production de xanthane a l'aide d'acides organiques non metabolisables
WO2001018226A1 (fr) * 1999-09-07 2001-03-15 Rhodia Chimie Procede de production d'exopolysaccharides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FERRO, A.A. ET AL.: "MESQUITE USE FOR THE PRODUCTION OF WATER BASE POLYMER POLYSACCHARIDES AS XANTHAN GUM", 6TH INTEMACIONAL CONFERENCE ON RENEWABLE RESOURCES AND BIOREFINERIES, 7 June 2010 (2010-06-07) - 9 June 2010 (2010-06-09), GERMANY *
INGLEZ DE SOUZA, J.S. ET AL., ENCICLOPEDIA AGRICOLA BRASILEIRA, vol. 1, 1995, ESALQ/UNIVERSIDADE DE SAO PAULO, pages 119 - 120 *
ROSEIRO, J.C. ET AL.: "BATCH AND FED-BATCH CULTIVATION OF XANTHOMONAS CAMPESTRIS IN CAROB EXTRACTS", LEBENSMITTEL-WISSENSCHAFT TECHNOLOGIE, vol. 25, no. 3, 1992, pages 289 - 293 *

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Publication number Publication date
BRPI0902629A2 (pt) 2011-03-29

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