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WO2011099021A1 - Composition et méthode de préparation d'un produit bactérien offrant un apport en phosphore à des plantes qui en sont dépourvus du fait d'un ph du sol plus élevé - Google Patents

Composition et méthode de préparation d'un produit bactérien offrant un apport en phosphore à des plantes qui en sont dépourvus du fait d'un ph du sol plus élevé Download PDF

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Publication number
WO2011099021A1
WO2011099021A1 PCT/IN2010/000179 IN2010000179W WO2011099021A1 WO 2011099021 A1 WO2011099021 A1 WO 2011099021A1 IN 2010000179 W IN2010000179 W IN 2010000179W WO 2011099021 A1 WO2011099021 A1 WO 2011099021A1
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composition
pac
culture
phosphate solubilizing
product according
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PCT/IN2010/000179
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English (en)
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Babubhai C. Patel
Chetan S. Patel
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like

Definitions

  • the present invention relates generally to a composition and method of making a bacterial based product that makes phosphorous available to plant which are unavailable due to higher soil PH.
  • a biological product for actively producing nitrogen and phosphate enrichment of soil comprising a nitrogen-fixing effective amount of Azospirillum sp. M32, an amount of Bacillus polymixa M7 sufficient to produce polymyxin, an anti-microbial peptide active against Gram negative bacteria which converts phosphorous materials fixed in the soil to which it is applied to a phosphate in plant-available forms, and a nutrient for the bacteria comprising a cellulosic material containing cellulose as an integral component selected from the group consisting of straw, xylan, hemicellulose and lignin, as well as other organic carbon or nitrogen materials.
  • compositions for providing phosphate fertilizer to the root zone of plants.
  • the composition comprises a microorganism capable of producing and secreting a solubilization agent, a carbon source for providing raw material for the microorganism to convert into the solubilization agent, and rock phosphate ore for providing a source of insoluble phosphate that is solubilized by the solubilization agent and released as soluble phosphate.
  • the composition is provided in a physical form, such as a granule, that retains the microorganism, carbon source, and rock phosphate ore, but permits water and soluble phosphate to diffuse into the soil.
  • a method of using the composition for providing phosphate fertilizer to plants is also disclosed.
  • VA mycorrhiza have growth-retardant activity for Poa annua and may be used to control the growth of P. annua in high quality sport or amenity turf consisting mainly of Agrostis or Festuca species.
  • Strains of VA mycorrhiza selected from genera Glomus, Acaulospora, Entrophosphora, Gigaspora, Scutellospora and Scierocytis may be used.
  • the VA mycorrhiza is applied to the soil as a formulated product in which the VA mycorrhizae constitute the principal component having biological activity on the growth of turf grass.
  • Dose rates of inoculum having an MPN of from about 200 to 1000 or more propagules per gram are effective and may be formulated with a clay or other solid carrier.
  • the amount of formulated product applied to the turf may be from about 5 g/m2 to about 1 kg/m2.
  • the infective prop are selected from fungal spores, mycelium, hyphae, and fragments of mycorrhiza infected roots. These may be applied to sport or amenity turf, or other grassy area in need of P. annua control, or an area in which high quality turf-grass is to be laid or sown.
  • the fungal inoculum is obtained by propagation of the microorganism in symbiotic association with the root systems of host plants either hydroponically, or in soil, depending upon the microorganism species used. Spores, cryoprotected hyphae and the seed of a vector plant are combined at low temperature with a cellulosic material which is used to form the packages. The formed packages are rapidly freeze-dried, and stored. The carrier system is inoculated into soil and, under proper environmental conditions, the plant seed germinates and the microorganisms become active and develop a symbiotic relationship with the growing plant.
  • the growing plant provides a vehicle for inoculating other nearby plant roots with the microorganisms.
  • the invention relates to eco-friendly compositions and methods for providing plant growth enhancing formulations comprising mixtures of microbial isolates.
  • numerous bacterial and fungal strains were isolated from a variety of soil types, from rhizospheres and from root nodules of leguminous plants, in designed combinations, for providing plant growth and plant productivity enhancing formulations.
  • These specifically designed polymicrobial formulations would further provide protection against plant pathogens lowering the need for nitrogen containing fertilizers, solubilize minerals, protect plants against pathogens, and make available to the plant valuable nutrients, such as phosphate, thus reducing and eliminating the need for using chemical fertilizers and chemical pesticides.
  • invention relates to product(s) containing alive microorganism ⁇ ) suitable for soil treatment, microorganisms multiplying under different climatic and natural circumstances, as well as the procedure for the production of the products, furthermore procedure for the treatment of the soil and plants with the products. More detailed, the invention relates to a procedure for preparing the products from any of the micro-organisms specified below, or from the mixture thereof. Furthermore, the invention relates to a procedure for the creation of the cultures of the micro-organisms to be used. Subject of the invention are the micro-organisms themselves, as well.
  • the invention relates to a procedure for the treatment of the soil and the plants with a product containing at least one of the micro-organisms Azospirillum brasilense ssp. SW51 (NCAIM /P/ B 001293), Azotobacter vinelandii spp. M657 (NCAIM IV I B 001291 ), Pseudomonas fluorescens var. SW1 1 (NCAIM /P/ B 001296), Bacillus polymyxa var. SW17 (NCAIM /P/ B 001295/, Bacillus megaterium var. M326 (NCAIM /P/ B 001301/ -, furthermore the products multiplying and existing in the environment of the plant in question, containing the listed micro-organisms and their production.
  • invention relates to a process for preparing a novel biofertiliser strains of nitrogen fixer and phosphate solubiiiser to promote soil health in particular for growth of tea plants comprising of nitrogen fixer and phosphate solubiiiser in equal proportion
  • the nitrogen fixer is prepared by the steps of a) transferring the stock culture Azotobacter Chroococcum (BAH) into mother culture modified Ashley's medium, b) allowing the mother culture medium to grow for 3-7 days, c) transferring the grown mother culture to main culture medium and d) allowing the main culture medium to grow ge for another 3-7 days to obtain Azotobacter choococcum (BAH) strains and in which the phosphate solubiiiser is prepared from as a mixture of Bacillus subtilis (BB 15), Pseudomonas Putida (BP19) Aspergillus awamori (FA16) and Aspergit
  • the known phosphate solubilizing bacteria product has different method of its preparation.
  • the present invention is based upon phosphate solubilizing bacteria from Bacillus megaterium , Pseudomonas putida that makes phosphorous available to plant, which are un available due to higher soil PH. It also increases efficiency of chemical fertilizer.
  • the inventor has invented a composition and method of making a bacterial based product that makes phosphorous available to plant, which are unavailable due to higher soil PH. It also increases efficiency of chemical fertilizer.
  • the phosphate solubilizing bacteria have the capability to change the insoluble form of phosphorus into soluble one. Accordingly, in one aspect, this invention provides the composition and method of preparing phosphate solubilizing bacteria from Bacillus megaterium and Pseudomonas putida that makes phosphorous available to plant, which are un available due to higher soil PH. It also increases efficiency of chemical fertilizer.
  • Pure culture of bacteria (Bacillus megatherium , Pseudomonas putida) is inoculated aseptically on plate having 20 ml PAC-09 Agar media; such two to three plates are generally inoculated. Such inoculated plate is maintained in BOD incubator at 27 ⁇ 1 °C for 6 to 7 days with 12/12 hour lighting cycle.
  • composition for one liter of PAC-09 agar media is 10 gm Sucrose, 0.1 gm MgS0 4 7H 2 0, 0.2 gm NaCl, 0.05 gm FeS0 4 , 5 gm Ca 3 (P0 4 ) 2 , 0.5 gm (NH ⁇ SC ⁇ , 0.2 gm KC1, 0.5 gm Yeast extract, 0.05 gm MnS0 4 , 20 gm Agar agar and 1000 ml distilled water).
  • Step-2 Starter culture (In 100 ml flask)
  • composition for one liter of PAC-09 broth media is 10 gm Sucrose, 0.1 gm MgS0 4 7H 2 0, 0.2 gm NaCl, 0.05 gm FeS0 4 , 5 gm Ca 3 (P0 4 ) 2 , 0.5 gm (NH 4 ) 2 S0 4 , 0.2 gm KC1, 0.5 gm Yeast extract, 0.05 gm MnS0 4 and 1000 ml distilled water).
  • Step-3 Growth culture (In 1000 ml flask)
  • step-2 After sufficient growth in 250 ml flask as specified in step-2, 50 ml culture from this grown culture is inoculated aseptically in 500 ml PAC-09 broth media in a 1000 ml capacity flask for further growth at 27 ⁇ 1 °C for at least 3 to 4 days on shaker with 150 RPM, such two flasks is prepared.
  • composition for one liter of PAC-09 broth media is 10 gm Sucrose, 0.1 gm MgS0 4 7H 2 0, 0.2 gm NaCl, 0.05 gm FeS0 4 , 5 gm Ca 3 (P0 4 ) 2 , 0.5 gm (NH 4 ) 2 S0 4 , 0.2 gm KC1, 0.5 gm Yeast extract, 0.05 gm MnS0 4 and 1000 ml distilled water).
  • Step-4 Seed Fermentor (In small fermentor of 10 lit capacities)
  • One (1) lit grown culture from step-3 is inoculated aseptically in small fermenter having 10 litter PAC-09 broth media, and allow for further growth at 27 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM.
  • Composition for one liter of PAC-09 broth media is 10 gm Sucrose, 0.1 gm MgS0 4 7H 2 0, 0.2 gm NaCl, 0.05 gm FeS0 4 , 5 gm Ca 3 (P0 4 ) 2 , 0.5 gm (Ntt ⁇ SC ⁇ , 0.2 gm KC1, 0.5 gm Yeast extract, 0.05 gm MnS0 4 and 1000 ml distilled water).
  • Step-5 Production Fermentor (In Big fermentor of 200 lit capacities)
  • Ten (10) lit culture grown in step-4 is inoculated aseptically in Big fermenter having 200 litter PAC-09 media
  • Composition for one liter of PAC-09 broth media is 10 gm Sucrose, 0.1 gm MgS0 4 7H 2 0, 0.2 gm NaCl, 0.05 gm FeS0 4 , 5 gm Ca 3 (P0 4 ) 2 , 0.5 gm (NH 4 ) 2 S0 4 , 0.2 gm KC1, 0.5 gm Yeast extract, 0.05 gm MnS0 4 and 1000 ml distilled water), and allow for further growth at 27 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM.
  • Step-6 Final out put from big fermentor mixed with starch @ 4 to 5 % which act as a stabilizer and melto dextrin @ 1 to 2 % which act as a drying agent or with 5 % skim milk powder and agitate continuously by using agitator.
  • Step-7 Mix culture prepared as per step-6, is then Spray dried at definite 160 °C in let and 70 to 72 °C out let temp in spray dryer unit.
  • Step-7 Collecting dry bacterial powder
  • Material dried in spray dryer unit is then collected in clean stainless steel vessels and stored at 5 °C up to further use.
  • dextrose @ 10 % 10 gm bacterial powder with 90 gm dextrose powder.
  • Material prepared as above is then packed in 100 gm, 250 gm and 500 gm packing by using pouch packing machine.
  • the final material prepared has to be used in following form:
  • the final material prepared has to be used in following form:

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention concerne une composition et une méthode de préparation d'un produit bactérien amélioré de solubilisation du phosphate, issu de Bacillus megaterium et de Pseudomonas putida, offrant un apport en phosphore à des plantes qui en sont dépourvus du fait d'un pH du sol plus élevé. La composition de l'invention augmente aussi le rendement des engrais chimiques.
PCT/IN2010/000179 2010-02-09 2010-03-24 Composition et méthode de préparation d'un produit bactérien offrant un apport en phosphore à des plantes qui en sont dépourvus du fait d'un ph du sol plus élevé Ceased WO2011099021A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN338MU2010 2010-02-09
IN338/MUM/2010 2010-02-09

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WO2011099021A1 true WO2011099021A1 (fr) 2011-08-18

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PCT/IN2010/000179 Ceased WO2011099021A1 (fr) 2010-02-09 2010-03-24 Composition et méthode de préparation d'un produit bactérien offrant un apport en phosphore à des plantes qui en sont dépourvus du fait d'un ph du sol plus élevé

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105112319A (zh) * 2015-07-08 2015-12-02 南京农业大学 一株假单胞菌属解磷细菌y2及其制备的生物有机肥和应用
CN108531424A (zh) * 2018-04-10 2018-09-14 暨南大学 一株用于土壤改良的高效溶磷菌及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6309440B1 (en) * 1998-08-25 2001-10-30 Thomas T. Yamashita Method and composition for promoting and controlling growth of plants
US6939688B1 (en) * 1999-11-01 2005-09-06 Soil Biogenics Ltd Biological addition to organic-mineral fertilizers
US20080248953A1 (en) * 2005-11-17 2008-10-09 Mcgill University Use of Bacteriocins For Promoting Plant Growth and Disease Resistance

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6309440B1 (en) * 1998-08-25 2001-10-30 Thomas T. Yamashita Method and composition for promoting and controlling growth of plants
US6939688B1 (en) * 1999-11-01 2005-09-06 Soil Biogenics Ltd Biological addition to organic-mineral fertilizers
US20080248953A1 (en) * 2005-11-17 2008-10-09 Mcgill University Use of Bacteriocins For Promoting Plant Growth and Disease Resistance

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NAUTIYAL C.S.: "An efficient microbiological growth medium for screening phosphate solubilizing microorganisms", FEMS MICROBIOLOGY LETTERS, vol. 170, no. 1, January 1999 (1999-01-01), pages 265 - 270, XP002511358 *
RODGRIGUEZ ET AL.: "Phosphate solubilizing bacteria and their role in plant growth promotion", BIOTECHNOLOGY ADVANCES, vol. 17, no. 4, 1999, pages 319 - 339, XP002553030 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105112319A (zh) * 2015-07-08 2015-12-02 南京农业大学 一株假单胞菌属解磷细菌y2及其制备的生物有机肥和应用
CN108531424A (zh) * 2018-04-10 2018-09-14 暨南大学 一株用于土壤改良的高效溶磷菌及其应用
CN108531424B (zh) * 2018-04-10 2020-08-07 暨南大学 一株用于土壤改良的溶磷菌及其应用

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