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WO2010125665A1 - Réacteur - Google Patents

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Publication number
WO2010125665A1
WO2010125665A1 PCT/JP2009/058457 JP2009058457W WO2010125665A1 WO 2010125665 A1 WO2010125665 A1 WO 2010125665A1 JP 2009058457 W JP2009058457 W JP 2009058457W WO 2010125665 A1 WO2010125665 A1 WO 2010125665A1
Authority
WO
WIPO (PCT)
Prior art keywords
reactor
gas
content liquid
dissolved oxygen
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2009/058457
Other languages
English (en)
Japanese (ja)
Inventor
陽一 石川
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Able Corp
Original Assignee
Able Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Able Corp filed Critical Able Corp
Priority to PCT/JP2009/058457 priority Critical patent/WO2010125665A1/fr
Publication of WO2010125665A1 publication Critical patent/WO2010125665A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates

Definitions

  • a microplate provided with a plurality of reactors as described above (in the microplate, the reactor is also referred to as a well and the microplate is also referred to as a cassette), for example, examination of reaction conditions such as biopolymers, chemical substances, Widely used in various biological and biochemical tests such as examination of culture conditions for cells, tissues, etc. of microorganisms, insects, animals and plants, drug discovery screening, cytotoxicity tests, pharmacokinetic tests, safety evaluation of compounds, etc.
  • reaction conditions such as biopolymers, chemical substances
  • various biological and biochemical tests such as examination of culture conditions for cells, tissues, etc. of microorganisms, insects, animals and plants, drug discovery screening, cytotoxicity tests, pharmacokinetic tests, safety evaluation of compounds, etc.
  • reactors such as 6, 24, 96, for example.
  • FIG. 3 is a partial sectional view for explanation showing the structure of the reactor.
  • the reactor 51 shown in the figure has a container shape made of a transparent material, and a pH sensor 53 (for example, , A fluorescence measurement system pH sensor) and a dissolved oxygen concentration sensor 54 (for example, a fluorescence measurement system dissolved oxygen concentration sensor) are provided in a recess formed in a state of being separated from each other with the center point of the bottom 52 interposed therebetween.
  • a vent hole 56 penetrating vertically is provided at the center of the bottom portion 52, and a vent filter such as a membrane is attached to the vent hole 56.
  • the pH of the content liquid 61 and the dissolved oxygen concentration are controlled based on the output signal by the above-described signal processing device (computer) in which the gas 55 whose supply gas type, flow rate, supply time, etc. are controlled is the bottom 52. It is performed by being supplied from the vent hole 56.
  • the gas 55 supplied in this way is dissolved in the content liquid 61 by stirring the reactor (in the case of a microplate, the whole) by an external stirring means, and the pH and dissolved oxygen concentration of the inner solution are reduced. Will be adjusted and controlled.
  • examples of the bottom shape of the reactor include a flat bottom, a round bottom, and a V bottom.
  • a flat bottom is preferable.
  • the volume of the reactor is not particularly limited and is selected and determined according to the purpose. For example, the reactor volume is 100 to 20,000 ⁇ l.
  • the material of the reactor is not particularly limited, and commonly used materials such as synthetic resins such as polystyrene, polyethylene, polypropylene, polyacrylonitrile, and polyethylene terephthalate, glass such as borosilicate glass and quartz glass, etc. Can be mentioned.
  • synthetic resins such as polystyrene, polyethylene, polypropylene, polyacrylonitrile, and polyethylene terephthalate
  • glass such as borosilicate glass and quartz glass, etc.
  • a transparent material is used for the bottom.
  • the reactor of the present invention controls the environment of the content liquid, particularly control of pH and dissolved oxygen concentration, and the most characteristic feature is that the bottom of the reactor and the content liquid stored in the reactor are moved up and down ( By establishing a vent pipe that penetrates in the axial direction) and opens in the space above the content liquid level, the gas that is controlled and supplied to this space is allowed to flow at the interface with the content liquid by stirring the content liquid. It is in a point where it is gradually dissolved in the content liquid by gas-liquid contact.
  • the structure characterized by the present invention other than the installation of a vent pipe inside the reactor, for example, a pH sensor provided at the bottom of the reactor, a dissolved oxygen concentration sensor, and a mechanism relating to external detection and control of information from these sensors
  • a vent pipe for example, a pH sensor provided at the bottom of the reactor, a dissolved oxygen concentration sensor, and a mechanism relating to external detection and control of information from these sensors
  • detection / control mechanism for example, a fluorometer, etc.
  • the reactor of the present invention can also be used for controlling dissolved carbon dioxide gas.
  • a measuring method of pH and dissolved oxygen concentration of the content liquid during control in addition to the method of measuring with a pH sensor or a dissolved oxygen concentration sensor provided on the inner surface of the bottom of the reactor as described in the above known example, for example, other known methods such as measuring by inserting a pH sensor or a dissolved oxygen concentration sensor into the content liquid from above the reactor can also be employed.
  • a ventilation filter can also be provided in the inside of the said ventilation pipe.
  • This ventilation filter is effective in preventing foreign matter (including microorganisms) from entering the contents from the external gas supply source side and intrusion of the contents liquid into the ventilation pipe.
  • the position where such a ventilation filter is provided may be an appropriate position inside the ventilation pipe, but for the purpose described above, it is considered more effective to be near the upper end opening of the ventilation pipe.
  • a well-known thing filter medium containing a film
  • porous urethane etc. can be mentioned.
  • the microplate of the present invention is provided with a plurality of reactors arranged in a line through support members, and is also referred to as a cassette as described above.
  • the alignment state is not particularly limited, but typically the whole is rectangular, and is arranged in a ratio of 2: 3 in the width direction and the longitudinal direction in the number (for example, In the case of 24, 4 ⁇ 6 rows, etc.).
  • microplate of the present invention having such a structure can be effectively used as a cassette capable of performing reactions, cultures, tests, and the like under a plurality of conditions simultaneously in the same manner as a conventional microplate. .
  • the reactor 1 of the present invention can prevent a decrease in the amount of the content liquid 5 due to evaporation. Furthermore, if the upper end opening 4b of the vent pipe 4 is positioned in the upper part of the space 6 and the gas 11 higher than the temperature of the content liquid 5 is supplied to the space 6, the convection of the gas 11 in the space 6 is prevented. Further, a decrease due to evaporation of the content liquid 5 is also prevented.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention porte sur un réacteur, qui effectue une régulation environnementale, par exemple, du pH ou de la concentration d'oxygène dissout d'un liquide contenu qui est reçu à l'intérieur de celui-ci, par délivrance d'un gaz à partir d'une source de gaz dans l'intérieur à partir du bas. Un tube de communication qui traverse le bas et le liquide contenu et qui s'ouvre au-dessus de la surface du liquide est disposé à l'intérieur du réacteur, et le gaz est délivré dans l'espace au-dessus de la surface du liquide par l'intermédiaire du tube de communication. Ceci permet la dissolution du gaz dans le liquide contenu de façon graduelle tout en empêchant la génération de bulles de gaz ou de mousse. De plus, la plage de fluctuation du pH ou de la concentration d'oxygène dissout du liquide contenu est réduite, et la régulation environnementale de celui-ci est rendue régulière.
PCT/JP2009/058457 2009-04-30 2009-04-30 Réacteur Ceased WO2010125665A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/JP2009/058457 WO2010125665A1 (fr) 2009-04-30 2009-04-30 Réacteur

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2009/058457 WO2010125665A1 (fr) 2009-04-30 2009-04-30 Réacteur

Publications (1)

Publication Number Publication Date
WO2010125665A1 true WO2010125665A1 (fr) 2010-11-04

Family

ID=43031832

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2009/058457 Ceased WO2010125665A1 (fr) 2009-04-30 2009-04-30 Réacteur

Country Status (1)

Country Link
WO (1) WO2010125665A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019151542A1 (fr) * 2018-02-05 2019-08-08 エイブル株式会社 Procédé de mesure d'échantillon, couvercle de plaque à puits multiples, kit de mesure d'échantillon et dispositif de mesure d'échantillon

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59194645U (ja) * 1983-06-09 1984-12-24 三和テッキ株式会社 油圧防振器のオイルリザ−バ
US20050176155A1 (en) * 2004-02-11 2005-08-11 Gener8, Inc. Well plate reactor
JP2008505629A (ja) * 2004-07-09 2008-02-28 チップマン テクノロジーズ オイ 細胞を培養するための基礎構造およびその使用
JP2008086264A (ja) * 2006-10-02 2008-04-17 Olympus Corp 細胞培養液供給排出装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59194645U (ja) * 1983-06-09 1984-12-24 三和テッキ株式会社 油圧防振器のオイルリザ−バ
US20050176155A1 (en) * 2004-02-11 2005-08-11 Gener8, Inc. Well plate reactor
JP2008505629A (ja) * 2004-07-09 2008-02-28 チップマン テクノロジーズ オイ 細胞を培養するための基礎構造およびその使用
JP2008086264A (ja) * 2006-10-02 2008-04-17 Olympus Corp 細胞培養液供給排出装置

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019151542A1 (fr) * 2018-02-05 2019-08-08 エイブル株式会社 Procédé de mesure d'échantillon, couvercle de plaque à puits multiples, kit de mesure d'échantillon et dispositif de mesure d'échantillon
JP2019135480A (ja) * 2018-02-05 2019-08-15 エイブル株式会社 試料計測方法、マルチウエルプレートの蓋、試料計測キット、及び、試料計測装置

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