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WO2010115424A1 - Micro-organismes méthanogènes permettant de générer du biogaz - Google Patents

Micro-organismes méthanogènes permettant de générer du biogaz Download PDF

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Publication number
WO2010115424A1
WO2010115424A1 PCT/DE2010/075035 DE2010075035W WO2010115424A1 WO 2010115424 A1 WO2010115424 A1 WO 2010115424A1 DE 2010075035 W DE2010075035 W DE 2010075035W WO 2010115424 A1 WO2010115424 A1 WO 2010115424A1
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Prior art keywords
microorganism
nucleotide sequence
microorganisms
culture
species
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English (en)
Inventor
Doris Schmack
Monika Reuter
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Schmack Biogas GmbH
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Schmack Biogas GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

  • the invention relates to processes for the production of biogas from biomass using methanogenic microorganisms and methanogenic microorganisms per se.
  • Biogas plants produce methane through a microbial decomposition process of organic substances.
  • the biogas is produced in a multi-stage process of fermentation or digestion by the activity of anaerobic microorganisms, i. in the absence of air.
  • the organic material used as fermentation substrate has a high molecular structure from a chemical point of view, which is degraded in the individual process steps of a biogas plant by metabolic activity of microorganisms to low molecular weight building blocks.
  • the populations of microorganisms which are active in the fermentation of the organic fermentation substrate have hitherto been insufficiently characterized.
  • exoenzymes e.g., cellulases, amylases, proteases, lipases
  • exoenzymes e.g., cellulases, amylases, proteases, lipases
  • the gaseous products formed besides consist predominantly of carbon dioxide.
  • hydrolysis products eg mono-, disaccharides, di-, oligopeptides, amino acids, glycerol, long-chain fatty acids
  • hydrolysis products eg mono-, disaccharides, di-, oligopeptides, amino acids, glycerol, long-chain fatty acids
  • short chain fatty or carboxylic acids such as butter , Propion and Acetic acid
  • short-chain alcohols such as ethanol
  • gaseous products hydrogen and carbon dioxide hydrogen and carbon dioxide.
  • acetogenesis such as acetic acid but also other substrates such as methanol and formate are converted by methane-forming organisms in the obligate anaerobic methanogenesis to methane and CO 2 .
  • the average amount of methane produced (methane productivity in standard cubic meters per m 3 working volume and day) of biogas plants is between 0.25 and 1, 1 Nm 3 CH 4 / m 3 d, with most plants having a methane productivity in the range from 0.50 to 0, 75 Nm 3 CH 4 / m 3 d (from "Results of the Biogas Measurement Program", 2005, Hrsgb, Agency for Renewable Resources, Gülzow)
  • Substrate-specific methane yields (expressed in standard cubic meters per ton fed substrate) have a very large value range between about 16 and 206 Nm 3 CH 4 A, because the substrates used to operate biogas plants are very different (eg manure or animal excreta, waste from food production or green waste, renewable raw materials such as silage maize or sugar beet pulp) and consequently very different energy contents (from "Results of the Biogas Measurement Program", 2005, Hrsgb.
  • the highest possible volume loading of the fermenter should be achieved.
  • the average room loads (expressed in kg of dry matter per cubic meter per day), under which biogas plants are operated, are 1 to 3 kg oTR / m 3 d, whereby one-stage systems usually have higher room loads than multi-stage systems and a room load of 5 , 7 kg oTR / m 3 d was the highest value that was measured (from "Results of the Biogas Measurement Program", 2005, Hrsgb. Agency for Renewable Resources, Gülzow).
  • the volume loading of a fermenter is the amount of substrate fed to the fermenter, expressed in kilograms of dry organic matter per cubic meter of fermenter volume and per day.
  • the amount of biogas produced depends strongly on the volume load of the fermenter, with increasing space load an increasingly larger amount of biogas is generated.
  • a high space load makes the process of biogas production increasingly economically viable, but on the other hand leads to an increasing destabilization of the biological processes of fermentation.
  • biogas is understood to mean the gaseous product of the anaerobic biodegradation of organic substrates, which generally contains about 45-70% of methane, 30-55% of carbon dioxide, and small amounts of nitrogen, hydrogen sulphide and other gases.
  • fermentation or “fermentation” in the context of the present invention include both anaerobic and aerobic metabolic processes which, under the action of microorganisms in a technical process from the supplied substrate to produce a product, e.g. Lead biogas.
  • a differentiation from the term “fermentation” is given insofar as it is exclusively an annaerobic process.
  • a “fermenter” is understood to mean the container in which the microbiological degradation of the substrate takes place with simultaneous formation of biogas.
  • the terms “reactor”, “fermenter” and “digester” are used interchangeably.
  • growth substrate or “substrate” in the context of the present invention means organic and biodegradable material which is added to the fermenter for fermentation.
  • Substrates can be renewable raw materials, farm fertilizers, substrates from the processing agricultural industry, organic municipal residues, slaughter residues or green waste.
  • Examples of the above-mentioned substrates are maize silage, rye silage, sugar beet pulp, molasses, grass silage, cattle or pig manure, cattle, pig, chicken or horse dung, spent grains, apple, fruit or vine pomace, cereal, potato or fruit vat.
  • fertilization residue or "digestate” is understood to be the residue of the biogas production leaving the fermenter and often stored in its own container.
  • volume loading is the amount of organic dry matter (oTS) supplied to the fermenter per day and cubic meter (m 3 ) working volume in kg.
  • organic dry substance (o-TS) is understood as meaning the anhydrous organic fraction of a substance mixture after removal of the inorganic constituents and drying at 105 ° C.
  • dry matter content is stated in% of the substrate.
  • constitutional time is understood to mean the average residence time of the substrate in the fermenter.
  • specific biogas yield or “specific methane yield” is used to denote the amount of biogas or methane produced (stated in standard cubic meters of Nm 3 gas) divided by the amount (as a rule per tonne) of organic dry substance used or substrate understood.
  • nucleotide sequence encompasses both the DNA sequence and the corresponding RNA sequence,
  • RNA sequences given in the invention using bases A, U, C, G also refer to the corresponding DNA sequences using bases A, T, C, G and vice versa.
  • nucleotide sequence of a microorganism by means of a standardized process by which the individual nucleotides of the DNA or RNA of the microorganism can be detected with high accuracy.
  • individual positions can repeatedly be present in a sequence for which the determination of the nucleotide present at the respective position was not possible with sufficient accuracy.
  • the letter "N” is indicated in the nucleotide sequence in the context of the present invention. If the letter "N" is subsequently used in a nucleotide sequence, this stands as an abbreviation for any conceivable nucleotide, that is to say A, U, G or C. in the case of a ribonucleic acid or for A, T, G or C in the case of a deoxyribonucleic acid.
  • nucleotide mutation means a change in the starting nucleotide sequence, whereby individual nucleotides or a plurality of nucleotide sequences which are directly consecutive or interrupted by unaltered nucleotides may be deleted, added (insertion or addition) or replaced by others (substitution)
  • substitution means any combination of individual variations called.
  • deletion means the removal of 1, 2 or more nucleotides from the respective starting sequence.
  • insertion or “addition” as used herein means the addition of 1, 2 or more nucleotides to the respective starting sequence.
  • substitution as used herein means the replacement of a nucleotide present at a particular position by another.
  • microorganism is understood to mean microscopically small organisms, which as a rule are single-celled organisms but may also be multicellular organisms Examples of microorganisms are bacteria, microscopic algae, fungi or protozoa.
  • the terms "genus” of microorganisms, "type” of microorganisms and “strain” of microorganisms are understood to mean the corresponding basic category of biological taxonomy, in particular the phylogenetic classification of genera, species and strains of
  • Microorganisms are u.a. identified and distinguished by their RNA sequences.
  • a certain species or a particular strain fall not only microorganisms with a very specific RNA sequence, but also to a certain extent their genetic variants, the genetic variance in the series strain, species, genus increases ,
  • culture refers to an accumulation of microorganisms under established conditions which ensure the growth or at least the survival of the microorganisms, for example enrichment cultures or pure cultures, liquid cultures as well as cultures on solid media such as nutrient media also permanent crops such as frozen glycerin cultures, immobilized cultures such as gel cultures or highly concentrated cultures such as cell pellets.
  • the term "pure culture" of a microorganism is understood to mean the progeny of a single cell, which is produced by a multi-stage process from a mixture isolated from various microorganisms.
  • the multi-step process involves the separation of a single cell from a cell population and requires that the colony resulting from the cell through growth and cell division also remain separate from other single cells or colonies. By careful separation of a colony, resuspension in liquid and repeated spreading, pure cultures of microorganisms can be selectively obtained.
  • the isolation of a pure culture can also be carried out in liquid nutrient media, provided that the desired organism outnumbered in the starting material.
  • mixed culture is understood to mean a mixture of different microorganisms, but natural populations of microorganisms are usually mixed cultures, but mixed cultures can also be produced artificially, for example by combining several pure cultures.
  • the object of the invention is to provide a process for the production of biogas, which allows an increased compared to the prior art yield of methane.
  • the question of whether the production of biogas in a given fermenter under certain conditions is satisfactory, can not be judged solely on the basis of the absolute amount of biogas produced.
  • the amount of biogas produced depends strongly on the amount of substrate supplied, especially on the amount of organic dry matter that is introduced into the fermenter. This is expressed in the measuring parameters "room load” and "specific gas yield", which thus constitute a particular importance for the efficiency of a plant. If instabilities of the fermentation process occur, such as a decrease in the pH, a strong increase in the formation of volatile fatty acids or the accumulation of inhibitors such as ammonia or hydrogen sulfide, the specific gas yield decreases.
  • composition of the microbial populations in the various fermentation substrates as well as the development of the organism composition during the fermentation process is largely unknown, but very variable and subject to a complicated dynamic process, which is also influenced by the respective process conditions.
  • various methods are known to those skilled in the art, for example, in the review article by Amann et al. (Microbiol. Review., 59, 143-169, 1995).
  • a preferred method of determining the microorganism composition independent of prior culture of the microorganisms is to construct a rDNA clone library (e.g., based on 16S rRNA) after nucleic acid extraction and PCR, which can then be sequenced.
  • a rDNA clone library e.g., based on 16S rRNA
  • the composition of the microbial population in the fermentation substrate can be determined, for example, by in situ hybridization with specific fluorescence-labeled oligonucleotide probes.
  • Suitable rRNA-based oligonucleotide probes are known from the review mentioned above or may be prepared, for example, by probe base (Loy et al., 2003, Nucleic Acids Res.
  • a quantitative determination of the proportion of individual microorganisms in the total population can be carried out in a suitable manner with the methods of quantitative dot blot, in situ hybridization or whole cell hybridization.
  • the addition of the bacterial culture can be carried out in the form of a liquid culture suspension, preferably in an enrichment or selection medium or in the form of dry, freeze-dried or moist cell pellets.
  • the cell concentrations achieved in enriched cultures are in a concentration range of about 10 6 cells per ml of culture to about 10 13 cells per ml of culture.
  • a preferred embodiment is also an immobilized culture of microorganisms.
  • natural or synthetic polymers can be used as carrier materials on which the particular microorganism is immobilized.
  • Gel-forming polymers are preferably used. These have the advantage that bacteria can be taken up or stored within the gel structure. Preferably, those materials are used which dissolve slowly in water or are degraded, so that the release of the respective microorganism takes place over a longer period of time.
  • suitable polymers are polyaniline, polypyrrole, polyvinylpyrrolidone, polystyrene, polyvinyl chloride, polyvinyl alcohol, polyethylene, polypropylene, epoxy resins, polyethyleneimines, polysaccharides such as agarose, alginate or cellulose, ethylcellulose, methylcellulose, carboxymethylethylcellulose, cellulose acetates, alkali cellulose sulfate, copolymers of polystyrene and maleic anhydride , Copolymers of styrene and methyl methacrylate, polystyrenesulfonate, polyacrylates and polymethacrylates, polycarbonates, polyesters, silicones, cellulose phthalate, proteins such as gelatin, gum arabic, albumin or fibrinogen, mixtures of gelatin and water glass, gelatin and polyphosphate, gelatin and copolymers of maleic anhydride and methyl vinyl ether, Cellulose acetate butyrate
  • Alginates as Immobilisate prove to be particularly advantageous because they do not have a negative influence on the activity of the microorganisms and because they are slowly degraded by other microorganisms. Due to the slow degradation of the alginate immobilizates, the trapped microorganisms are gradually released.
  • the microorganisms are mixed with a polymer gel and then cured in a suitable hardener solution. For this they are first mixed with a gel solution and then dropped into a hardener solution of suitable height.
  • a suitable hardener solution of suitable height.
  • microorganisms described in more detail below have properties, as a result of which the addition of the microorganisms during a fermentation to produce biogas has a positive effect on the amount of biogas produced, especially biomethane, which significantly increases the profitability of the respective plants.
  • microorganisms suitable for this purpose can take place at any desired point in time of the fermentation process; in particular, the microorganisms can be used to inoculate fermentation substrate during initial startup or restart of a fermenter. All of the microorganisms described in more detail below can be used to inoculate fermentation substrate.
  • the microorganisms according to the invention may be added in the form of a culture once or several times at regular or irregular intervals, but preferably weekly or monthly, more preferably daily or twice weekly, in a suitable concentration and amount. Suitable concentrations of microorganisms and amounts added are explained in the specific embodiments.
  • the microorganisms are added even in case of disturbances in the fermentation process to stabilize the fermentation.
  • Such disorders can be detected early by monitoring certain characteristic parameters of the fermentation.
  • Characteristic parameters provide information about the quality of an ongoing fermentation process for the production of biogas.
  • Such characteristic parameters are not only the amount of biogas produced and the methane content of the biogas produced but also, for example, the hydrogen content of the biogas produced, the pH of the fermentation substrate, the redox potential of the fermentation substrate, the carboxylic acid content of the fermentation substrate, the proportions of various carboxylic acids in the fermentation substrate, the hydrogen content of the fermentation substrate, the proportion of dry matter in the fermentation substrate, the proportion of the organic dry matter in the fermentation substrate, the viscosity of the fermentation substrate and the volume loading of the fermentation reactor. All the microorganisms described in more detail below can be added in case of disturbances of the fermentation process to stabilize the fermentation.
  • additional biomass is added to the fermentation reactor in a timely manner to the addition of the microorganisms described below.
  • Timely addition of additional biomass may occur within a period of 1 second to 3 days after addition of microorganisms, or it may occur simultaneously with the addition of microorganisms.
  • the space load in the fermentation reactor by continuous The addition of new substrate continuously increased or kept approximately constant, the fermentation at all room loads, preferably at a space load of ⁇ 0.5 kg of organic dry matter per m 3 and day [kg oTS / m 3 d], more preferably at a space load of ⁇ 4.0 kg oTS / m 3 d and particularly preferably at a volume load of ⁇ 8.0 kg oTS / m 3 d, which is more than double the increase in average room load compared to the current state of the art.
  • the space load in the fermentation reactor is continuously increased by the continuous addition of biomass.
  • fermentation substrate and microorganisms are added continuously.
  • the continuous operation of a fermentation reactor should result in a stable microbial biocenosis to a continuous production of biogas, the exposure of the substrate addition to the fermentation should be reduced as a result of a process disturbance.
  • all the microorganisms described in more detail below can be used.
  • a fermentation microorganism may be added to the fermentation substrate at regular intervals At regular intervals leads to an increase in the wardzelliere and thus to an improved course of methane formation.Also in this embodiment of the present invention, all microorganisms described in more detail below can be used.
  • the production of biogas from biomass takes place with constant mixing of the fermentation substrate. Due to the constant mixing of the fermentation substrate, the added cultures of microorganisms can be better distributed in the fermentation substrate. In addition, the biogas formed can be better removed from the fermentation process.
  • the constant mixing of the fermentation substrate also leads to a uniform heat distribution in the fermentation reactor.
  • Measurements of the temperature in the fermentation reactor carried out at periodic intervals, but also continuously were showed that the fermentation substrate is fermented efficiently in a temperature range from 2O 0 C and 8O 0 C, preferably at about 35 0 C to 60 0 C, more preferably at 4O 0 C to 50 0 C. These temperature ranges are therefore preferred in the context of the present invention in connection with all the microorganisms described in more detail below.
  • the last stage of the fermentation process namely the formation of methane by methanogenic microorganisms, particularly efficient at elevated temperatures.
  • All embodiments of the present invention are not limited to one-step processes for the production of biogas.
  • the use of all microorganisms described in more detail below can also be carried out in two or more stages.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanoculleus strengensis.
  • the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanoculleus strengensis causes a significant increase in the amount of biogas produced.
  • the amount of methane formed can also be increased by increasing the volume load, with the addition of microorganisms of the species Methanoculleus strengensis preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanoculleus strengensis therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • a microorganism of the species Methanoculleus strengensis is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanoculleus strengensis.
  • microorganisms of the species could Methanoculleus strengensis only in trace amounts of less than 10 '4% share of the total number of detected present microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanoculleus strengensis can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • microorganisms of the species Methanoculleus strengensis are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanoculleus strengensis. If, in addition to the determination of the number of microorganisms of the species Methanoculleus strengensis, the total number of microorganisms is also determined, the percentage of microorganisms of the species Methanoculleus strengensis in the culture can be stated as a percentage. Microorganisms of the species Methanoculleus strengensis are in a mixed culture then the predominantly present type of microorganisms, if they have the highest percentage of the various types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanoculleus strengensis at least 10 '4%, especially at least 10' 2%, more preferably at least 1% of the total number of existing in the added to the fermentation substrate culture microorganisms.
  • microorganisms of the species Methanoculleus strengensis account for at least 10%, in particular at least 50%, particularly preferably at least 90%, of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanoculleus strengensis is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanoculleus strengensis is added as a constituent of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanoculleus strengensis is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanoculleus strengensis is between 1 CT 8 % and 50% of the total number of microorganisms present in the fermentation substrate accounts.
  • the proportion of the microorganism of the species Methanoculleus strengensis is between 1 CT 8 % and 50% of the total number of microorganisms present in the fermentation substrate accounts.
  • an addition of very different amounts of microorganisms can be necessary to achieve the desired effect.
  • a microorganism of the species Methanoculleus is particularly preferred laubensis in an amount added to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanoculleus strengensis between 10 -6% and 25% of the total number present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanoculleus is particularly preferred laubensis in an amount to the fermentation substrate is added that, after addition, the proportion of the microorganism of the species Methanoculleus strengensis between 10 "4% and 10% of the total number present in the fermentation substrate microorganisms accounts.
  • microorganism of the species Methanoculleus strengensis admitted that makes up after addition of the content of the microorganism of the species Methanoculleus strengensis at present in the fermentation substrate of microorganisms between 10 -3% and 1% of the total in an amount to the fermentation substrate.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Methanoculleus sp. dm 2.
  • a microorganism of the strain Methanoculleus sp. dm2 added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain krchaeon clone GZK7.
  • a microorganism of the strain krchaeon clone GZK7 is therefore preferably added.
  • the microorganism of the species Methanoculleus strengensis is a Microorganism of the strain Archaeon clone 5.5ft C121A.
  • a microorganism of the strain Archaeon clone 5.5ft C121A is added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Archaeon clone 5.5ft C124A.
  • a microorganism of the strain Archaeon clone 5.5ft C124A is added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Archaeon clone 5.5ft C141A.
  • a microorganism of the strain Archaeon clone 5.5ft C141A is added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Archaeon clone A52.
  • a microorganism of the strain Archaeon clone A52 is therefore preferably added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Archaeon clone MCSArc_B6.
  • a microorganism of the strain Archaeon clone MCSArc_B6 is therefore preferably added.
  • the microorganism of the species Methanoculleus strengensis is a
  • Microorganism of strain Euryarchaeote clone BSA1A-03. which deals with the addition of a microorganism of the type Methanoculleus laubensis, so preferably a microorganism of the strain Euryarchaeote clone BSA 1 A-03 is added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Euryarchaeote clone BSA2A-02.
  • a microorganism of the strain Euryarchaeote clone BSA2A-02 is added.
  • the microorganism of the species Methanoculleus strengensis is a microorganism of the strain Euryarchaeote clone MAA04.
  • a microorganism of the strain Euryarchaeote clone MAA04 is added.
  • the present invention also encompasses the use of a microorganism of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • a microorganism of the strain Methanoculleus sp. dm2 used.
  • a microorganism of the strain Archaeon clone GZK7 is used for the fermentative production of biogas from biomass.
  • a microorganism of the strain Archaeon clone 5.5ft C121A is also preferred for the fermentative production of biogas from biomass.
  • Also preferred for the fermentative production of biogas from biomass is a microorganism of the strain Archaeon clone 5.5ft C124A used. Also preferred for fermentative production of biogas from biomass, a microorganism of the strain Archaeon clone 5.5ft Ci '41 A is used. Also preferably, a microorganism of the strain Archaeon clone A52 is used for the fermentative production of biogas from biomass. Also preferably, a microorganism of the strain Archaeon clone MCSArc_B6 is used for the fermentative production of biogas from biomass.
  • a microorganism of the strain Euryarchaeote clone BSA 1A-03 is used.
  • a microorganism of the strain Euryarchaeote clone BSA2A-02 is used.
  • a microorganism of the strain Euryarchaeote clone MAA04 is used for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG7 obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 4 comprises 974 nucleotides.
  • Methanoculleus sp.dm2 was identified as the next relative. A comparison of the sequences revealed that there are a total of 26 exchanges of nucleotides or deletions. At a length of the specific nucleotide sequence of 974 nucleotides of methanoculleus strengensis SBG7 and a length of the reference sequence of 968 nucleotides, a 97.31% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.31% sequence identity with the nucleotide sequence of SEQ ID NO: 4. More preferably, the nucleotide sequence contains a sequence range greater than 97.4% or greater than 97.5% or greater than 97.6% or greater than 97.7% or greater than 97.8% or greater than 97.9%. or more than 98.0% sequence identity with the nucleotide sequence SEQ ID NO: 4, and more preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 4.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 4, and more preferably the nucleotide sequence contains a sequence region having more than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 4.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 4.
  • SEQ ID NO: 4 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or at 23 positions or at 24 positions or at 25 positions or at 26 nucleotide mutations.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism having a nucleotide sequence containing a sequence region greater than 97.31 is present % Sequence identity with the nucleotide sequence of SEQ ID NO: 4, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.4% or greater than 97.5% or greater than 97 , 6% or more than 97.7% or more than 97.8% or more than 97.9% or more than 98.0% sequence identity with the nucleotide sequence SEQ ID NO: 4. More preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 4.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 4 has. Most preferably, the nucleotide sequence contains a sequence region having more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 4.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 4.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 4 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 4 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 4 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG12 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 9 comprises 1119 nucleotides.
  • the next related sequence identified was the sequence of the non-cultured archaeon clone GZK7.
  • a match of 97.54% At a length of the particular nucleotide sequence of Methanoculleus strengensis SBG12 of 1 1 19 nucleotides and a length of the reference sequence of 1099 nucleotides calculated using the FASTA algorithm a match of 97.54%.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region that exceeds 97.54%.
  • nucleotide sequence SEQ ID NO. 9 has. More preferably, the nucleotide sequence contains a sequence range greater than 97.6% or greater than
  • nucleotide sequence SEQ ID NO: 9 98.1% or more than 98.2% sequence identity with the nucleotide sequence SEQ ID NO: 9, and more preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 9.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 9, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 9. According to a most preferred In an embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 9.
  • SEQ ID NO: 9 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or at 23 positions or at 24 positions or at 25 positions or at 26 positions or at 27 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 97.54 % Sequence identity with the nucleotide sequence of SEQ ID NO: 9, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.6% or greater than 97.7% or greater than 97 , 8% or more than 97.9% or more than 98.0% or more than 98.1% or more than 98.2% sequence identity with the nucleotide sequence of SEQ ID NO: 9. More preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 9.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 9 has.
  • the nucleotide sequence most preferably contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 9.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 9.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 9 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 9 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 9 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG13 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 10 comprises 1 123 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Archaeon clone 5.5ft C121A.
  • a match of 98.04% is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 98.04% sequence identity with the nucleotide sequence of SEQ ID NO: 10. More preferably, the nucleotide sequence contains a sequence range greater than 98.1% or greater than 98.2% or greater than 98.3% or greater than 98.4% or greater than 98.5% or greater than 98.6%. or more than 98.7% sequence identity with the nucleotide sequence SEQ ID NO: 10, and more preferably, the nucleotide sequence contains a sequence region having more than 98.8% sequence identity with the nucleotide sequence SEQ ID NO: 10.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 10, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 10.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 10.
  • SEQ ID NO: 10 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on There are 22 nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 98.04 % Sequence identity with the nucleotide sequence SEQ ID NO: 10, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present, which has a nucleotide sequence having a sequence range greater than 98.1% or greater than 98.2% or greater than 98.3% or greater than 98.4% or greater than 98.5% or greater than 98.6% or more than 98.7% has sequence identity with the nucleotide sequence SEQ ID NO: 10. More preferably, the nucleotide sequence contains a sequence region having more than 98.8% sequence identity with the nucleotide sequence of SEQ ID NO: 10.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 10 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 10.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 10.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 10 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 10 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 10 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in one of the above - mentioned
  • Methanoculleus strengensis method described in more detail for the fermentative production of biogas from biomass.
  • microorganisms Methanoculleus strengensis SBG14 also obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis. The determined
  • 16S rRNA sequence SEQ ID NO: 1 comprises 1 133 nucleotides.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region that exceeds 97.53%.
  • sequence identity with the nucleotide sequence SEQ ID NO. 1 1 has. More preferably, the nucleotide sequence contains a sequence range greater than 97.6% or greater than
  • nucleotide sequence SEQ ID NO: 1 98.1% or more than 98.2% sequence identity with the nucleotide sequence SEQ ID NO: 1 1, and more preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 1 1.
  • the microorganism has a nucleotide sequence which contains a sequence region which has more than 99.0% sequence identity with the nucleotide sequence SEQ ID No. 11, and more preferably the nucleotide sequence contains a sequence region which exceeds 99.5%. Sequence identity with the nucleotide sequence SEQ ID NO. 1 1 has. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 11.
  • SEQ ID NO: 1 can be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine Positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or at 22 Positions or at 23 positions or at 24 positions or at 25 positions or at 26 positions or at 27 positions or at 28 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 97.53 % Sequence identity with the nucleotide sequence SEQ ID NO: 1 1, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.6% or more
  • nucleotide sequence SEQ ID NO: 1 98.1% or greater than 98.2% sequence identity with the nucleotide sequence SEQ ID NO: 1 1.
  • nucleotide sequence contains a sequence region which has more than 98.5% sequence identity with the nucleotide sequence SEQ ID No. 11.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 1 1 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 11.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 11.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 1 1 for the fermentative production of biogas from biomass. It is preferable to use a Microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 1 1 in a method described in more detail above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 1 1 and wherein the microorganism comprises at least 10 '4 % of the total number of microorganisms present in the culture.
  • a culture of microorganisms for fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 1 1 and wherein the microorganism is at least 10 '4 % of the total number of microorganisms present in the culture, in a process described in more detail above in connection with microorganisms of the species Methanoculleus strengensis, for the fermentative production of biogas from biomass.
  • microorganisms Methanoculleus strengensis SBG15 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis. The determined
  • 16S rRNA sequence SEQ ID NO: 12 comprises 801 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Archaeon clone 5.5ft C124A.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region that exceeds 96.88%.
  • nucleotide sequence SEQ ID NO. 12 has. More preferably, the nucleotide sequence contains a sequence range greater than 96.9% or greater than
  • 97.4% or more than 97.5% has sequence identity with the nucleotide sequence SEQ ID NO: 12, and more preferably the nucleotide sequence contains a sequence region having more than 98.0% or more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 12
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 12, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence SEQ ID NO: 12. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 12.
  • SEQ ID NO: 12 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or 23 positions or 24 positions or 25 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 96.88 % Sequence identity with the nucleotide sequence of SEQ ID NO: 12, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 96.9% or more
  • nucleotide sequence SEQ ID NO: 12 contains a sequence region having greater than 98.0% or greater than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 12.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 12 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 12.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 12.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 12 for fermentative purposes
  • Microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 12 in a method described in more detail above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 12 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG31 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 28 comprises 556 nucleotides.
  • the sequence of the uncultured Archaeon clone 5.5ft C141A was identified.
  • a 99.10% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 99.10% sequence identity with the nucleotide sequence of SEQ ID NO: 28. More preferably, the nucleotide sequence contains a sequence region that is greater than 99.2% or greater than 99.3% or greater than 99.4% or greater than 99.5% or greater than 99.6% sequence identity to the nucleotide sequence of SEQ ID NO 28, and particularly preferably, the nucleotide sequence contains a sequence region which has more than 99.7% sequence identity with the nucleotide sequence SEQ ID No. 28.
  • the microorganism has a nucleotide sequence containing a sequence region having greater than 99.8% sequence identity with the nucleotide sequence SEQ ID NO: 28, and more preferably, the nucleotide sequence contains a sequence region having greater than 99.9% sequence identity having the nucleotide sequence of SEQ ID NO: 28. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 28.
  • nucleotide mutations may be present at one or two positions or at three positions or at four positions or at five positions relative to the starting nucleotide sequence SEQ ID NO: 28.
  • the meaning of the term “nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 99.10 having% sequence identity with the nucleotide sequence SEQ ID NO. 28, wherein the microorganism makes up at least 10 '4% of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.2% or greater than 99.3% or greater than 99 , 4% or more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 28.
  • the nucleotide sequence contains a sequence region having greater than 99.6% or greater than 99.7% sequence identity with the nucleotide sequence of SEQ ID NO: 28.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence region having more than 99.8% sequence identity with the nucleotide sequence SEQ ID NO. 28 has. Most preferably, the nucleotide sequence contains a sequence region having more than 99.9% sequence identity with the nucleotide sequence of SEQ ID NO: 28.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 28.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 28 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 28 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 28 and wherein the microorganism is at least 10 '. 4 % of the total number of microorganisms present in the culture.
  • a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 28 and wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture, in one above in connection with microorganisms of the species Methanoculleus strengensis described method for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG30 also obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 27 comprises 909 nucleotides.
  • the sequence of the uncultured Archaeon clone A52 was identified.
  • a 95.82% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 95.82% sequence identity with the nucleotide sequence of SEQ ID NO: 27. More preferably, the nucleotide sequence contains a sequence range greater than 95.9% or greater than 96.0% or greater than 96.1% or greater than 96.2% or greater than 96.3% or greater than 96.4%. or more than 96.5% sequence identity with the nucleotide sequence SEQ ID NO: 27, and most preferably, the nucleotide sequence contains a sequence region having greater than 97.0% or greater than 98.0% sequence identity with the nucleotide sequence SEQ ID NO: 27 ,
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 27, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 27.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 27.
  • SEQ ID NO: 27 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or at ten Positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or 22 positions or at 23 positions or 24 positions or 25 positions or 26 positions or 27 positions or 28 positions or 29 positions or 30 positions or 31 positions or 32 positions or 33 positions or 34 positions or 35 Positions or at 36 positions or at 37 positions or at 38 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 95,82 % Sequence identity with the nucleotide sequence SEQ ID NO: 27, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 95.9% or more
  • nucleotide sequence SEQ ID NO: 27 contains a sequence region having greater than 97.0% or greater than 98.0% sequence identity with the nucleotide sequence SEQ ID NO:
  • No. 27 has.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 27 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 27.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is Microorganisms include a microorganism having a nucleotide sequence containing a sequence region corresponding to the nucleotide sequence of SEQ ID NO: 27.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 27 for fermentative
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 27 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • a culture of microorganisms for fermentative production of biogas from biomass wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 27 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG23 also obtained from the fermentation substrate of a post-fermenter, were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 20 comprises 801 nucleotides.
  • the next related sequence identified was the sequence of the non-cultured archaeon clone MCSArc_B6. A comparison of the sequences revealed that there are a total of 33 exchanges of nucleotides or deletions.
  • the FASTA algorithm gives a 95.88% match.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 95.88% sequence identity with the nucleotide sequence of SEQ ID NO: 20.
  • the nucleotide sequence contains a sequence range greater than 95.9% or greater than 96.0% or greater than 96.1% or greater than 96.2% or greater than 96.3% or greater than 96.4% or greater having 96.5% sequence identity with the nucleotide sequence SEQ ID NO: 20, and more preferably, the nucleotide sequence contains a sequence region having more than 97.0% or more than 98.0% sequence identity with the nucleotide sequence SEQ ID NO: 20.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 20, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 20.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 20.
  • SEQ ID NO: 20 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or 23 positions or 24 positions or 25 positions or 26 positions or 27 positions or 28 positions or 29 positions or 30 positions or 31 positions or 32 positions or 33 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present, which contains a
  • nucleotide sequence containing a sequence range greater than 95.88%
  • Microorganism accounts for at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism is present which has a nucleotide sequence with a sequence range greater than 95.9% or greater than 96.0% or greater than 96 , 1% or more than 96.2% or more than 96.3% or more than 96.4% or more than 96.5% sequence identity with the nucleotide sequence SEQ ID NO: 20.
  • the nucleotide sequence contains a sequence region having greater than 97.0% or greater than 98.0% sequence identity with the nucleotide sequence SEQ ID NO: 20.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 20 has. Most preferably, the nucleotide sequence contains a sequence region that has more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 20.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID NO.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 20 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 20 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 20 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 20 and wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture, in one above in connection with microorganisms of the species Methanoculleus strengensis described method for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG28 also obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 25 comprises 801 nucleotides.
  • the next related sequence identified was the non-cultured Euryarchaeote clone BSA 1A-03.
  • a 99.63% agreement is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 99.63% sequence identity with the nucleotide sequence of SEQ ID NO: 25. More preferably, the nucleotide sequence contains a sequence region that is greater than 99.65% or greater than 99.67% or greater than 99.69% or greater than 99.70% or greater than 99.72% sequence identity to the nucleotide sequence of SEQ ID NO 25, and particularly preferably, the nucleotide sequence contains a sequence region having more than 99.75 sequence identity with the nucleotide sequence SEQ ID NO: 25.
  • the microorganism has a nucleotide sequence containing a sequence region having greater than 99.8% sequence identity with the nucleotide sequence SEQ ID NO: 25, and more preferably the nucleotide sequence contains a sequence region having greater than 99.9% sequence identity having the nucleotide sequence SEQ ID NO: 25.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 25.
  • nucleotide mutations may be present at one or two positions or at three positions relative to the starting nucleotide sequence of SEQ ID NO: 25.
  • the meaning of the term “nucleotide mutation” is explained in the "Definitions” section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 99.63 % Sequence identity with the nucleotide sequence SEQ ID NO: 25, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.65% or greater than 99.67% or greater than 99 , 69% or more than 99.70% sequence identity with the nucleotide sequence SEQ ID NO: 25.
  • the nucleotide sequence contains a sequence region having greater than 99.72% or greater than 99.75% sequence identity with the nucleotide sequence SEQ ID NO: 25.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence region having more than 99.8% sequence identity with the nucleotide sequence SEQ ID NO. 25 has. Most preferably, the nucleotide sequence contains a sequence region having more than 99.9% sequence identity with the nucleotide sequence SEQ ID NO: 25.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 25.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 25 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 25 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No. 25 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG29 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 26 comprises 679 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Euryarchaeote clone BSA2A-02.
  • the FASTA algorithm gives a 99.26% match.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 99.26% sequence identity with the nucleotide sequence of SEQ ID NO: 26. More preferably, the nucleotide sequence contains a sequence region that is greater than 99.28% or greater than 99.3% or greater than 99.4% or greater than 99.5% or greater than 99.6% sequence identity to the nucleotide sequence of SEQ ID NO , 26, and more preferably, the nucleotide sequence contains a sequence region having more than 99.7% sequence identity with the nucleotide sequence SEQ ID NO: 26.
  • the microorganism has a nucleotide sequence which contains a sequence range which exceeds more than 99.8%.
  • nucleotide sequence SEQ ID NO. 26 Sequence identity with the nucleotide sequence SEQ ID NO. 26, and more preferably, the nucleotide sequence contains a sequence region having more than 99.9% sequence identity having the nucleotide sequence of SEQ ID NO: 26. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 26.
  • nucleotide mutations may be present at one or two positions or at three positions or at four positions or at five positions relative to the starting nucleotide sequence SEQ ID NO: 26.
  • the meaning of the term “nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 99,26 % Sequence identity with the nucleotide sequence SEQ ID NO: 26, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.28% or greater than 99.3% or greater than 99 , 4% or more than 99.5% sequence identity with the
  • Nucleotide sequence has SEQ ID NO: 26. Particularly preferably, the
  • Nucleotide sequence has a sequence region which has more than 99.6% or more than 99.7% sequence identity with the nucleotide sequence SEQ ID No. 26.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence region having more than 99.8% sequence identity with the nucleotide sequence SEQ ID NO. 26 has. Most preferably, the nucleotide sequence contains a sequence region having more than 99.9% sequence identity with the nucleotide sequence SEQ ID NO: 26.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is Microorganisms include a microorganism having a nucleotide sequence containing a sequence region corresponding to the nucleotide sequence of SEQ ID NO: 26.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 26 for fermentative
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 26 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus strengensis SBG32 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 29 comprises 935 nucleotides.
  • the next related sequence was a partial sequence of the uncultivated Euryarchaeote clone MAA04.
  • a comparison of the sequences revealed that a total of 23 exchanges of nucleotides or deletions are present.
  • a 97.54% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.54% sequence identity with the nucleotide sequence of SEQ ID NO: 29.
  • the nucleotide sequence contains a sequence range greater than 97.6% or greater than 97.7% or greater than 97.8% or greater than 97.9% or greater than 98.0% or greater than 98.1% or greater 98.2% has sequence identity with the nucleotide sequence SEQ ID NO: 29, and more preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 29.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 29, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 29.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 29.
  • SEQ ID NO: 29 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or at 23 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 97.54 having% sequence identity with the nucleotide sequence SEQ ID NO. 29, wherein the microorganism makes up at least 10 '4% of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.6% or greater than 97.7% or greater than 97 , 8% or more than 97.9% or more than 98.0% or more than 98.1% or more than 98.2% sequence identity with the nucleotide sequence SEQ ID NO: 29 having.
  • the nucleotide sequence contains a sequence region which has more than 98.5% sequence identity with the nucleotide sequence SEQ ID No. 29.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is
  • Microorganisms present a microorganism having a nucleotide sequence with a
  • Sequence region has more than 99.0% sequence identity with the nucleotide sequence
  • nucleotide sequence contains a
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 29.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 29 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 29 in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 29 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus strengensis for the fermentative production of biogas from biomass.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • it is a pure culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanoculleus chikugoensis.
  • the amount of biogas formed can be significantly increased by the addition of microorganisms of the species Methanoculleus chikugoensis to the fermentation substrate.
  • the addition of microorganisms of the species Methanoculleus chikugoensis causes a significant increase in the amount of biogas produced.
  • the amount of methane formed can also be increased by increasing the volume load, with the addition of microorganisms of the species Methanoculleus chikugoensis preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanoculleus chikugoensis therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • a microorganism of the species Methanoculleus chikugoensis is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanoculleus chikugoensis.
  • microorganisms of the species could be detected by Methanoculleus present microorganisms chikugoensis only in trace amounts of less than 10 '4% share of the total number. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanoculleus chikugoensis can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • microorganisms of the species Methanoculleus chikugoensis are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanoculleus chikugoensis.
  • the proportion of microorganisms of the species Methanoculleus chikugoensis in the culture can be specified in percent. Microorganisms of the species Methanoculleus chikugoensis are in a mixed culture then predominantly present type of microorganisms, if they have the highest percentage of the various types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanoculleus chikugoensis at least 10 '4%, especially at least 10 "2%, more preferably at least 1% of the total number of the added to the fermentation substrate culture microorganisms present. According to further preferred embodiments make microorganisms of the species Methanoculleus chikugoensis at least 10%, in particular at least 50%, particularly preferably at least 90% of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanoculleus chikugoensis is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanoculleus chikugoensis is added as a component of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanoculleus chikugoensis is added in an amount to the fermentation substrate, so that after addition of the proportion of the microorganism of the species Methanoculleus chikugoensis between 10 ⁇ 8 % and 50% of the total number of present in the fermentation substrate microorganisms accounts.
  • an addition of very different amounts of microorganisms can be necessary to achieve the desired effect, an addition of very different amounts of microorganisms.
  • a microorganism of the species Methanoculleus chikugoensis is particularly preferably added in an amount to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanoculleus chikugoensis between 10 -6% and 25% of the total number present in the fermentation substrate microorganisms. More preferably, the microorganism of the species Methanoculleus chikugoensis is added in an amount to the fermentation substrate such that, after addition, the proportion of the microorganism of the species Methanoculleus chikugoensis is between 10 '4 % and 10% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanoculleus chikugoensis is very particularly preferably added in an amount to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanoculleus chikugoensis between 10 "3% and 1% of the total number present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanoculleus chikugoensis is a microorganism of the strain Methanoculleus chikugoensis MG62.
  • a microorganism of the strain Methanoculleus chikugoensis MG62 is added.
  • the present invention also encompasses the use of a microorganism of the species Methanoculleus chikugoensis for the fermentative production of biogas from biomass.
  • a microorganism of the strain Methanoculleus chikugoensis MG62 is used for the fermentative production of biogas from biomass.
  • the microorganisms methanoculleus chikugoensis SBG5 obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 2 comprises 1,124 nucleotides.
  • Methanoculleus chikugoensis MG62 was identified as the next relative.
  • the 98.93% agreement is calculated using the FASTA algorithm.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region of greater than 98.93%. Sequence identity with the nucleotide sequence SEQ ID NO. 2 has. More preferably, the nucleotide sequence contains a sequence range greater than 99.0% or greater than 99.1% or greater than 99.2% or greater than 99.3% or greater than 99.4% or greater than 99.5%. or more than 99.6% has sequence identity with the nucleotide sequence SEQ ID NO: 2, and more preferably, the nucleotide sequence contains a sequence region having more than 99.7% sequence identity with the nucleotide sequence SEQ ID NO: 2.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.8% sequence identity with the nucleotide sequence SEQ ID NO: 2, and more preferably the nucleotide sequence contains a sequence region having greater than 99.9% sequence identity having the nucleotide sequence of SEQ ID NO: 2.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 2.
  • SEQ ID NO: 2 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or nucleotide mutations at ten positions or at eleven positions or at twelve positions.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present, which contains a
  • nucleotide sequence containing a sequence region greater than 98.93%
  • Microorganism accounts for at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.0% or greater than 99.1% or greater than 99 , 2% or more than 99.3% or more than 99.4% or more than 99.5% or more than 99.6% sequence identity with the nucleotide sequence SEQ ID NO: 2 having. More preferably, the nucleotide sequence contains a sequence region having more than 99.7% sequence identity with the nucleotide sequence SEQ ID NO: 2.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is
  • Microorganisms present a microorganism having a nucleotide sequence with a
  • Sequence region has more than 99.8% sequence identity with the nucleotide sequence
  • nucleotide sequence contains a
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 2.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 2 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 2 in a method described above in connection with microorganisms of the species Methanoculleus chikugoensis for the fermentative production of biogas from biomass.
  • the present invention also includes the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 2 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus chikugoensis for the fermentative production of biogas from biomass.
  • the microorganisms make said, characterized in terms of their nucleotide sequence microorganisms at least 10 '2%, preferably to at least 1% of the total present in the culture of microorganisms.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • Biogas from biomass which is a pure culture of a microorganism as characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is added to a microorganism of the species Methanoculleus chikugoensis.
  • microorganism of the species Methanoculleus chikugoensis is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanoculleus chikugoensis make up at least 10 '4 % of the total number of microorganisms present in the culture.
  • Biomass is continuously increased.
  • Methanoculleus chikugoensis take place continuously.
  • Methanoculleus chikugoensis accounts for between 10 8 % and 50% of the total number of microorganisms present in the fermentation substrate.
  • microorganism of the species Methanoculleus chikugoensis is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus chikugoensis between 10 "6 % and 25% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus chikugoensis is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus chikugoensis between 10 '4 % and 10% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus chikugoensis is a microorganism of the strain Methanoculleus chikugoensis MG62.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 98.93% sequence identity with the nucleotide sequence SEQ ID No. 2.
  • Microorganism according to claim 24 characterized in that the nucleotide sequence contains a sequence region which has more than 99.2% sequence identity with the nucleotide sequence SEQ ID No. 2.
  • Microorganism according to claim 26 characterized in that the nucleotide sequence contains a sequence region which has more than 99.6% sequence identity with the nucleotide sequence SEQ ID No. 2.
  • nucleotide sequence contains a sequence region which has more than 99.8% sequence identity with the nucleotide sequence SEQ ID No. 2.
  • Microorganism according to claim 28 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 2.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanoculleus marisnigri.
  • the addition of microorganisms of the species Methanoculleus marisnigri to the fermentation substrate the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanoculleus marisnigri causes a significant increase in the amount of biogas produced.
  • the amount of methane produced can also be increased by increasing the volume load, and the addition of microorganisms of the species Methanoculleus marisnigri prevents the fermentation process from becoming unstable under the changed conditions.
  • the use of microorganisms of the species Methanoculleus marisnigri therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • Methods Methanoculleus marisnigri are also to be understood as meaning the “Methanogenium marisnigri” (Bacterial Nomenclature Up-To-Date, Approved List of the DSMZ - German Collection for Microorganisms and Cell Cultures GmbH, as of March 2010) of biogas by fermentation of organic substrates, microorganisms of the species Methanoculleus marisnigri are not yet known.
  • a microorganism of the species Methanoculleus marisnigri is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanoculleus marisnigri.
  • microorganisms of the species could Methanoculleus marisnigri only in trace amounts of less than 10 '4% share of the total number of detected present microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanoculleus marisnigri can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • microorganisms of the species Methanoculleus marisnigri are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanoculleus marisnigri.
  • the proportion of microorganisms of the species Methanoculleus marisnigri in the culture may be expressed as a percentage.
  • Microorganisms of the species Methanoculleus marisnigri are in a mixed culture then predominantly present type of microorganisms, if they have the highest percentage of the various types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanoculleus marisnigri at least 10 '4%, especially at least 10' 2%, more preferably at least 1% of the total number of existing in the added to the fermentation substrate culture microorganisms.
  • microorganisms of the species Methanoculleus marisnigri make up at least 10%, in particular at least 50%, particularly preferably at least 90%, of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanoculleus marisnigri is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanoculleus marisnigri is added as a constituent of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanoculleus marisnigri is added in an amount to the fermentation substrate, so that after addition of the proportion of the microorganism of the species Methanoculleus marisnigri between 10 "8 % and 50% of the total number present in the fermentation substrate
  • a microorganism of the species Methanoculleus marisnigri is particularly preferably added in an amount to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanoculleus marisnigri between 10 -6% and 25% of the total number present in the fermentation substrate microorganisms. More preferably, the microorganism of the species Methanoculleus marisnigri is added to the fermentation substrate in an amount added that after addition of the proportion of the microorganism of the species Methanoculleus marisnigri is between 10 '4 % and 10% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanoculleus marisnigri is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanoculleus marisnigri is between 10 -3 % and 1% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanoculleus marisnigri is a microorganism of the strain Methanoculleus marisnigri JRI.
  • a microorganism of the strain Methanoculleus marisnigri JR1 it is therefore preferable to add a microorganism of the strain Methanoculleus marisnigri JR1.
  • the present invention also encompasses the use of a microorganism of the species Methanoculleus marisnigri for the fermentative production of biogas from biomass.
  • a microorganism of the strain strain Methanoculleus marisnigri JR1 is used for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus marisnigri SBG6 obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 3 comprises 1440 nucleotides.
  • Methanoculleus marisnigri JR1 was identified as the next relative.
  • a 97.36% match is calculated using the FASTA algorithm.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region exceeding 97.36%.
  • nucleotide sequence SEQ ID NO. 3 has. More preferably, the nucleotide sequence contains a sequence range greater than 97.4% or greater than
  • the microorganism has a nucleotide sequence which contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 3, and more preferably the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence Nucleotide sequence SEQ ID NO. 3 has. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 3.
  • SEQ ID NO: 3 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or at ten
  • nucleotide mutations Positions or at 35 positions or at 36 positions or at 37 positions or at 38 positions nucleotide mutations.
  • nucleotide mutation The meaning of the term “nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein a microorganism having a nucleotide sequence containing a sequence region greater than 97.36 is present in the culture of microorganisms % Sequence identity with the nucleotide sequence of SEQ ID NO: 3, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.4% or greater than 97.5% or greater than 97 , 6% or more than 97.7% or more than 97.8% or more than 97.9% or more than 98% sequence identity with the nucleotide sequence SEQ ID NO: 3. More preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 3.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence region having more than 99% sequence identity with the nucleotide sequence SEQ ID NO: 3 , Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 3.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 3.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 3 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 3 in a method described above in connection with microorganisms of the species Methanoculleus marisnig ⁇ for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 3 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus marisnigri for the fermentative production of biogas from biomass.
  • microorganisms make said, characterized in terms of their nucleotide sequence microorganisms at least 10 '2%, preferably at least 1% of the total number of microorganisms present in the culture.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • it is a pure culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is a microorganism of the type
  • Methanoculleus marisnigri is added.
  • microorganism of the species Methanoculleus marisnigri is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanoculleus marisnigri at least 10 '4 % of
  • microorganisms of the species Methanoculleus marisnigri account for at least 50% of the total number of microorganisms present in the culture.
  • Biomass is added to the fermentation reactor.
  • Methanoculleus marisnig ⁇ take place continuously.
  • Methanoculleus marisnigri represents between 10 "8 % and 50% of the total number of microorganisms present in the fermentation substrate.
  • microorganism of the species Methanoculleus marisnigri is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus marisnigri between 10 "6 % and 25% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus marisnigri is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus marisnigri between 10 '4 % and 10% of the total in the Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus marisnigri is a microorganism of the strain Methanoculleus marisnigri JR1.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 97.36% sequence identity with the nucleotide sequence SEQ ID No. 3.
  • Microorganism according to claim 24 characterized in that the nucleotide sequence contains a sequence region which has more than 98% sequence identity with the nucleotide sequence SEQ ID No. 3.
  • Microorganism according to claim 26 characterized in that the nucleotide sequence contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 3.
  • Microorganism according to claim 27 characterized in that the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 3.
  • Microorganism according to claim 28 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 3.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanoculleus thermophilus.
  • the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanoculleus thermophilus causes a significant increase in the amount of biogas formed.
  • the amount of methane formed can also be increased by increasing the volume load, with the addition of microorganisms of the species Methanoculleus thermophilus preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanoculleus thermophilus therefore leads to an improvement in efficiency and efficiency of biogas plants.
  • a microorganism of the species Methanoculleus thermophilus is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanoculleus thermophilus.
  • microorganisms of the species could be detected by Methanoculleus present microorganisms only in trace amounts of less than 10 '4% share of the total number thermophilus. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanoculleus thermophilus can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • Methanoculleus thermophilus Since the already mentioned various positive effects on the fermentation process are associated with the microorganism species Methanoculleus thermophilus, this type of microorganism should be present in the added culture in a concentration exceeding the natural abundance. Of course, mixed cultures of any composition can be used for the addition. All that is required is that the species Methanoculleus thermophilus is present in a concentration which is enriched in relation to the natural occurrence.
  • the proportion of microorganisms of the species Methanoculleus thermophilus in a mixture can be specifically identified with the aid of fluorescence-labeled oligopeptides.
  • microorganisms of the species Methanoculleus thermophilus are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanoculleus thermophilus.
  • the percentage of microorganisms of the species Methanoculleus thermophilus in the culture can be stated as a percentage.
  • Microorganisms of the species Methanoculleus thermophilus are in a mixed culture then the predominantly present type of microorganisms, if they have the highest percentage of the various types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanoculleus thermophilus is at least 10 '4%, especially at least 10' 2%, more preferably at least 1% of the total number existing in the added to the fermentation substrate culture microorganisms.
  • microorganisms of the species Methanoculleus thermophilus account for at least 10%, in particular at least 50%, particularly preferably at least 90%, of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanoculleus thermophilus is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanoculleus thermophilus is added as a constituent of at least one immobilized culture of microorganisms.
  • Microorganisms the amount of isolated from their natural occurrence
  • Microorganisms is insufficient, is usually made an increase in the form of a culture.
  • the addition of the microorganisms to the fermentation substrate of a fermenter is most easily achieved in the form of an immobilized culture of
  • Microorganisms is made.
  • the microorganism of the species Methanoculleus thermophilus is added in an amount to the fermentation substrate, so that after addition of the proportion of microorganism of the species Methanoculleus thermophilus between 10 ⁇ 8 % and 50% of the total number of present in the fermentation substrate microorganisms accounts.
  • an addition of very different amounts of microorganisms can be necessary to achieve the desired effect, an addition of very different amounts of microorganisms.
  • a microorganism of the species Methanoculleus thermophilus is particularly preferably added in an amount to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanoculleus thermophilus between 10 -6% and 25% of the total number present in the fermentation substrate microorganisms. More preferably, the microorganism of the species Methanoculleus thermophilus is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanoculleus thermophilus is between 10 '4 % and 10% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanoculleus thermophilus is very particularly preferably added in an amount to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanoculleus thermophilus between 10 "3% and 1% of the total number present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanoculleus thermophilus is a microorganism of the strain Archaeon clone HDBW-WA02.
  • a microorganism of the strain Archaeon clone HDBW-WA02 is added.
  • the present invention also encompasses the use of a microorganism of the species Methanoculleus thermophilus for the fermentative production of biogas from biomass.
  • a microorganism of the strain Archaeon clone HDBW-WA02 is used for the fermentative production of biogas from biomass.
  • the microorganisms Methanoculleus thermophilus SBG27 obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 24 comprises 1124 nucleotides.
  • the next related sequence identified was the non-cultured Archaeon clone HDBW-WA02.
  • the FASTA algorithm gives a 98.96% match.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region of greater than 98.96%. Sequence identity with the nucleotide sequence SEQ ID NO. 24 has. More preferably, the nucleotide sequence contains a sequence range greater than 99.0% or greater than 99.1% or greater than 99.2% or greater than 99.3% or greater than 99.4% or greater than 99.5%. or more than 99.6% sequence identity with the nucleotide sequence SEQ ID NO: 24, and more preferably, the nucleotide sequence contains a sequence region having more than 99.7% sequence identity with the nucleotide sequence SEQ ID NO: 24.
  • the microorganism has a nucleotide sequence which contains a sequence region which has more than 99.8% sequence identity with the nucleotide sequence SEQ ID No. 24, and more preferably the nucleotide sequence contains a sequence region which has more than 99.9% sequence identity having the nucleotide sequence of SEQ ID NO: 24.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 24.
  • SEQ ID NO: 24 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or nucleotide mutations at ten positions or eleven positions.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 98.96 % Sequence identity with the nucleotide sequence SEQ ID NO: 24, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.0% or greater than 99.1% or greater than 99 , 2% or more than 99.3% or more than 99.4% or more than 99.5% or more than 99.6% sequence identity with the nucleotide sequence SEQ ID NO: 24. More preferably, the nucleotide sequence contains a sequence region having more than 99.7% sequence identity with the nucleotide sequence SEQ ID NO: 24.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence region having more than 99.8% sequence identity with the nucleotide sequence SEQ ID NO. 24 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.9% sequence identity with the nucleotide sequence SEQ ID No. 24.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 24.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 24 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 24 in a method described above in connection with microorganisms of the species Methanoculleus thermophilus for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 24 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanoculleus thermophilus for the fermentative production of biogas from biomass.
  • microorganisms make said, characterized in terms of their nucleotide sequence microorganisms at least 10 '2%, preferably at least 1% of the total number of microorganisms present in the culture.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • it is a pure culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is a microorganism of the type
  • Methanoculleus thermophilus is added.
  • microorganism of the species Methanoculleus thermophilus is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanoculleus thermophilus at least 10 '4 % of
  • microorganisms of the species Methanoculleus thermophilus make up at least 50% of the total number of microorganisms present in the culture.
  • Biomass is continuously increased.
  • Methanoculleus thermophilus take place continuously.
  • Methanoculleus thermophilus accounts for between 10 "8 % and 50% of the total number of microorganisms present in the fermentation substrate.
  • microorganism of the species Methanoculleus thermophilus is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus thermophilus between 10 "6 % and 25% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus thermophilus is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus thermophilus between 10 '4 % and 10% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus thermophilus is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanoculleus thermophilus between 10 '3 % and 1% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanoculleus thermophilus is a microorganism of the strain Archaeon clone HDBW-WA02.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 98.96% sequence identity with the nucleotide sequence SEQ ID No. 24.
  • Microorganism according to claim 24 characterized in that the nucleotide sequence contains a sequence region which has more than 99.2% sequence identity with the nucleotide sequence SEQ ID No. 24.
  • Microorganism according to claim 26 characterized in that the nucleotide sequence contains a sequence region which has more than 99.6% sequence identity with the nucleotide sequence SEQ ID No. 24.
  • Microorganism according to claim 28 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 24.
  • a method for producing biogas from biomass in a fermentation reactor wherein the biomass is added to a microorganism of the species Methanoculleus thermophilus.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanobacterium formicicum.
  • the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanobacterium formicicum causes a significant increase in the amount of biogas produced.
  • the amount of methane formed can also be increased by increasing the space load, with the addition of microorganisms of the species Methanobacterium formicicum preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanobacterium formicicum therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • strains of microorganisms fall under the term "species Methanobacterium formicicum."
  • microorganisms of the species Methanobacterium formicicum are hitherto unknown.
  • a microorganism of the species Methanobacterium formicicum in the form of a culture of Microorganisms added which consists predominantly of microorganisms of the species Methanobacterium formicicum.
  • microorganisms of the species Methanobacterium formicicum In fermentation substrates of biogas plants could microorganisms of the species Methanobacterium formicicum only in trace amounts of less than 10 '4% share of the total number of detected present microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • the addition of the culture of Methanobacterium formicicum can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • Methanobacterium formicicum Since the already mentioned various positive effects on the fermentation process are associated with the microorganism species Methanobacterium formicicum, this type of microorganism should be present in the added culture in a concentration exceeding the natural abundance. Mixed cultures containing Methanobacterium formicicum along with any other microorganisms may also be used for the addition. All that is required is that the species Methanobacterium formicicum is present in a concentration which is enriched in relation to the natural occurrence.
  • Microorganisms of the species Methanobacterium formicicum can be identified in a mixture.
  • microorganisms of the species Methanobacterium formicicum are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanobacterium formicicum.
  • Methanobacterium formicicum also determines the total number of microorganisms, the percentage of microorganisms of the species Methanobacterium formicicum in the culture can be stated in percent. Microorganisms of the species Methanobacterium formicicum in a mixed culture are then the predominant species of microorganisms when they have the highest percentage of the various species present in the mixed culture
  • microorganisms of the species Methanobacterium formicicum make microorganisms of the species Methanobacterium formicicum at least 10 '4%, especially at least 10' 2%, more preferably at least 1% of the total number of existing in the added to the fermentation substrate culture microorganisms.
  • microorganisms of the species Methanobacterium formicicum account for at least 10%, in particular at least 50%, particularly preferably at least 90%, of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanobacterium formicicum is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanobacterium formicicum is added as a constituent of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanobacterium formicicum is added in an amount to the fermentation substrate, so that after addition of the proportion of the microorganism of the species Methanobacterium formicicum between 10 '8 % and 50% of the total number present in the fermentation substrate
  • a microorganism of the species Methanobacterium formicicum is particularly preferred in an amount added to the fermentation substrate, that after addition the content of the microorganism of the species Methanobacterium formicicum between 10 -6% and 25% of the total number of microorganisms present in the fermentation substrate by weight.
  • the microorganism of the species Methanobacterium formicicum is added in an amount added to the fermentation substrate that after addition of the proportion of the microorganism of the species Methanobacterium formicicum accounts for between 10 '4 % and 10% of the total number of present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanobacterium formicicum is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanobacterium formicicum accounts for between 10 '3 % and 1% of the total number of present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanobacterium formicicum is a microorganism of the strain Methanobacterium sp., Clone SMS-sludge-11.
  • a microorganism of the strain Methanobacterium sp., Cloned SMS sludge-11 is added.
  • the microorganism of the species Methanobacterium formicicum is a microorganism of the strain Methanobacterium sp. 169.
  • a microorganism of the strain Methanobacterium sp. 169 added.
  • the microorganism of the species Methanobacterium formicicum is a microorganism of the strain Archaeon clone CG-4.
  • a microorganism of the strain Archaeon clone CG-4 is added.
  • the present invention also encompasses the use of a microorganism of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • a microorganism of the strain Methanobacterium sp. Clone SMS-sludge-11 used.
  • Also preferred for fermentative production of biogas from biomass is a microorganism of the strain Methanobacterium sp. 169 used.
  • a microorganism of the strain Archaeon clone CG-4 is used for the fermentative production of biogas from biomass.
  • Methanobacterium formicicum SBG4 were subjected to sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 1 comprises 1 133 nucleotides.
  • the sequence of uncultivated Methanobacterium sp. clone As the next related 16S rRNA sequence, the sequence of uncultivated Methanobacterium sp. clone
  • SMS-sludge-11 identified. A comparison of the sequences revealed that a total of 26
  • Length of the reference sequence of 1 128 nucleotides is calculated using the FASTA algorithm a 97.70% agreement.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.70% sequence identity with the nucleotide sequence of SEQ ID NO: 1. More preferably, the nucleotide sequence contains a sequence range greater than 97.8% or greater than 97.9% or greater than 98.0% or greater than 98.1% or greater than 98.2% or greater than 98.3%. or more than 98.4% or more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 1, and more preferably, the nucleotide sequence contains a sequence region having more than 99% sequence identity with the nucleotide sequence SEQ ID NO: 1.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 1, and more preferably the nucleotide sequence contains a sequence region having greater than 99.8% sequence identity having the nucleotide sequence of SEQ ID NO: 1.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 1.
  • SEQ ID NO: 1 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or 23 positions or 24 positions or 25 positions or 26 Positions nucleotide mutations present.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present, which contains a
  • Microorganism accounts for at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.8% or greater than 97.9% or greater than 98 , 0% or more than 98.1% or more than 98.2% or more than 98.3% or more than 98.4% or more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 1.
  • the nucleotide sequence contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 1.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range of more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO. 1 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.8% sequence identity with the nucleotide sequence SEQ ID No. 1.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 1.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 1 for fermentative purposes
  • a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 1 has been described in a method described above in connection with microorganisms of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 1 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • the microorganisms Methanobacterium formicicum SBG8 obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the 16S rRNA sequence SEQ ID NO: 5 comprises 903 nucleotides.
  • the next relative was Methanobacterium sp. 169 identified.
  • a comparison of the nucleotide sequences revealed that a total of 22 exchanges of nucleotides or deletions are present.
  • a 97.56% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.56% sequence identity with the nucleotide sequence of SEQ ID NO: 5. More preferably, the nucleotide sequence contains a sequence range greater than 97.6% or greater than 97.7% or greater than 97.8% or greater than 97.9% or greater than 98.0% or greater than 98.1%.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 5 and more preferably the nucleotide sequence contains a sequence region having greater than 99.8% sequence identity having the nucleotide sequence of SEQ ID NO: 5.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 5.
  • SEQ ID NO: 5 can be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or at ten
  • nucleotide mutation Positions nucleotide mutations present. The meaning of the term “nucleotide mutation” is explained in the “Definitions” section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present, which contains a
  • Microorganism accounts for at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.6% or greater than 97.7% or greater than 97 , 8% or more than 97.9% or more than 98.0% or more than 98.1% or more than 98.2% or more than 98.3% or more than 98.4% or more than 98, 5% sequence identity with the nucleotide sequence SEQ ID NO: 5.
  • the nucleotide sequence contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 5.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is Microorganisms, a microorganism having a nucleotide sequence having a sequence region having more than 99.5% sequence identity with the nucleotide sequence of SEQ ID NO: 5. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.8% sequence identity with the nucleotide sequence SEQ ID No. 5.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 5.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 5 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 5 in a method described above in connection with microorganisms of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 5 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • the microorganisms Methanobacterium formicicum SBG25 obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 22 comprises 914 nucleotides.
  • the closest related sequence identified was a 16S rRNA sequence of the uncultured Archaeon clone CG-4.
  • a comparison of the sequences revealed that a total of 27 exchanges of Nucleotides or deletions.
  • a 97.05% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.05% sequence identity with the nucleotide sequence of SEQ ID NO: 22. More preferably, the nucleotide sequence contains a sequence range greater than 97.1% or greater than 97.2% or greater than 97.3% or greater than 97.4% or greater than 97.5% or greater than 97.6%.
  • nucleotide sequence SEQ ID NO: 22 or more than 97.7% or more than 97.8% or more than 97.9% or more than 98.0% sequence identity with the nucleotide sequence SEQ ID NO: 22, and more preferably the nucleotide sequence contains a sequence region greater than 98.5% or greater than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 22.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 22, and more preferably the nucleotide sequence contains a sequence region having greater than 99.8% sequence identity having the nucleotide sequence SEQ ID NO: 22.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 22.
  • SEQ ID NO: 22 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or at 23 positions or at 24 positions or at 25 positions or at 26 positions or at 27 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present, which contains a A nucleotide sequence containing a sequence region having more than 97.05% sequence identity with the nucleotide sequence of SEQ ID NO: 22, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism which has a nucleotide sequence with a sequence range greater than 97.1% or greater than 97.2% or greater than 97 , 3% or more than 97.4% or more than 97.5% or more than 97.6% or more than 97.7% or more than 97.8% or more than 97.9% or more than 98, 0% sequence identity with the nucleotide sequence SEQ ID NO: 22.
  • the nucleotide sequence contains a sequence region which has more than 98.5% or more, ie 99.0% sequence identity, with the nucleotide sequence SEQ ID No. 22.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range of more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO. 22 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.8% sequence identity with the nucleotide sequence SEQ ID No. 22.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 22.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 22 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 22 in a method described above in connection with microorganisms of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • the present invention also includes the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No. 22 and wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanobacterium formicicum for the fermentative production of biogas from biomass.
  • microorganisms make said, characterized in terms of their nucleotide sequence microorganisms at least 10 '2%, preferably to at least 1% of the total present in the culture of microorganisms.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • it is a pure culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is added to a microorganism of the species Methanobacterium formicicum.
  • microorganism of the species Methanobacterium formicicum is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanobacterium formicicum account for at least 10 '4 % of the total number of microorganisms present in the culture.
  • the method according to claim 4 characterized in that constitute in the culture of microorganisms of the species Methanobacterium formicicum at least 10% of the total number of microorganisms present in the culture.
  • microorganism of the species Methanobacterium formicicum is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanobacterium formicicum between 10 "8 % and 50% the total number of microorganisms present in the fermentation substrate.
  • microorganism of the species Methanobacterium formicicum is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanobacterium formicicum between 10 "6 % and 25% of the total in Fermentation substrate present
  • microorganisms 19. The method according to claim 18, characterized in that the microorganism of the species Methanobacterium formicicum is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanobacterium formicicum between 10 '4 % and 10% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanobacterium formicicum is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanobacterium formicicum between 10 "3 % and 1% of the total in Fermentation substrate present
  • microorganism of the species Methanobacterium formicicum is a microorganism of the strain Methanobacterium sp. 169 acts.
  • microorganism of the species Methanobacterium formicicum is a microorganism of the strain Archaeon clone CG-4.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 97.70% sequence identity with the nucleotide sequence SEQ ID No. 1.
  • Microorganism according to claim 28 characterized in that the nucleotide sequence contains a sequence region which has more than 98% sequence identity with the nucleotide sequence SEQ ID No. 1.
  • nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 1.
  • the microorganism according to claim 32 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 1.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which is more than
  • nucleotide sequence contains a sequence region which has more than 98% sequence identity with the nucleotide sequence SEQ ID No. 5.
  • nucleotide sequence contains a sequence region which has more than 98.5% sequence identity with the nucleotide sequence SEQ ID No. 5.
  • Microorganism according to claim 36 characterized in that the nucleotide sequence contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 5.
  • nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 5.
  • Microorganism according to claim 38 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 5.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 97.05% sequence identity with the nucleotide sequence SEQ ID No. 22.
  • Microorganism according to claim 40 characterized in that the nucleotide sequence contains a sequence region which has more than 98% sequence identity with the nucleotide sequence SEQ ID No. 22.
  • the microorganism according to claim 41 characterized in that the nucleotide sequence contains a sequence region which has more than 98.5% sequence identity with the nucleotide sequence SEQ ID No. 22.
  • the microorganism according to claim 42 characterized in that the nucleotide sequence contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 22.
  • microorganism according to claim 43 characterized in that the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 22.
  • Microorganism according to claim 44 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 22.
  • culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass characterized in that in the culture of microorganisms, a microorganism according to claims 28 to 45 is present, wherein the microorganism at least 10 '4 % of the total in the culture microorganisms present.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanosarcina acetivorans.
  • the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanosarcina acetivorans causes a significant increase in the amount of biogas produced.
  • the amount of methane formed can also be increased by increasing the space load, with the addition of microorganisms of the species Methanosarcina acetivorans preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanosarcina acetivorans therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • a microorganism of the species Methanosarcina acetivorans is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanosarcina acetivorans.
  • microorganisms of the species Methanosarcina acetivorans could be detected only in trace amounts of less than 10 "4% share of the total number of present microorganisms.
  • Methanosarcina acetivorans can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • microorganisms of the species Methanosarcina acetivorans are preferably added in the form of cultures of microorganisms to the fermentation substrate, wherein the cultures of microorganisms consist predominantly of microorganisms of the species Methanosarcina acetivorans.
  • the proportion of microorganisms of the species Methanosarcina acetivorans in culture in percent. Microorganisms of the species Methanosarcina acetivorans are in a mixed culture then the predominant species of microorganisms, if they have the highest percentage of the various types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanosarcina acetivorans at least 10 '4%, especially at least 10' 2%, more preferably at least 1% of the total number existing in the added to the fermentation substrate culture microorganisms.
  • microorganisms of the species Methanosarcina acetivorans account for at least 10%, in particular at least 50%, particularly preferably at least 90%, of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanosarcina acetivorans is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanosarcina acetivorans is added as a component of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanosarcina acetivorans is added in an amount to the fermentation substrate, so that after addition of the proportion of the microorganism of the species Methanosarcina acetivorans between 10 '8 % and 50% of the total number present in the fermentation substrate
  • microorganisms constitutes microorganisms.
  • an addition of very different amounts of microorganisms More preferably, a microorganism of the species Methanosarcina acetivorans is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina acetivorans is between 10 6 % and 25% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina acetivorans is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina acetivorans is between 10 -4 % and 10% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina acetivorans is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina acetivorans between 1 CT is 3 % and 1% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina acetivorans is a microorganism of the strain Archaeon clone 5.5ft C85A.
  • a microorganism of the strain Archaeon clone 5.5ft C85A is added.
  • the present invention also encompasses the use of a microorganism of the species Methanosarcina acetivorans for the fermentative production of biogas from biomass.
  • a microorganism of the strain strain Archaeon clone 5.5ft C85A is used for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina acetivorans SBG19 obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 16 comprises 919 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Archaeon clone 5.5ft C85A.
  • a 97.39% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.39% sequence identity with the nucleotide sequence of SEQ ID NO: 16.
  • the nucleotide sequence contains a sequence range greater than 97.4% or greater than 97.5% or greater than 97.6% or greater than 97.7% or greater than 97.8% or greater than 97.9% or greater when 98% has sequence identity with the nucleotide sequence SEQ ID NO: 16, and more preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 16.
  • the microorganism has a nucleotide sequence which contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 16, and more preferably the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence Nucleotide sequence SEQ ID NO. 16 has. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 16.
  • SEQ ID NO: 16 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or 23 positions or 24 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism having a nucleotide sequence containing a sequence region greater than 97.39 is present % Sequence identity with the nucleotide sequence SEQ ID NO: 16, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.4% or more
  • nucleotide sequence SEQ ID NO: 16 More preferably, the nucleotide sequence contains a sequence region having more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 16.
  • the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass comprises a microorganism having a nucleotide sequence with a sequence region having more than 99% sequence identity with the nucleotide sequence SEQ ID NO: 16 , Most preferably, the nucleotide sequence contains a sequence region having more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 16.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 16.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 16 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 16 in a method described above in connection with microorganisms of the species Methanosarcina acetivorans for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 16 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture. It is preferable to use a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanosarcina acetivorans for the fermentative production of biogas from biomass.
  • make said characterized in terms of their nucleotide sequence microorganisms at least 10 '2%, preferably to at least 1% of the total present in the culture of microorganisms.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • it is a pure culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is added to a microorganism of the species Methanosarcina acetivorans.
  • microorganism of the species Methanosarcina acetivorans is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanosarcina acetivorans make up at least 10 '4 % of the total number of microorganisms present in the culture.
  • the method according to claim 3 characterized in that constitute in the culture of microorganisms microorganisms of the species Methanosarcina acetivorans at least 1% of the total number of microorganisms present in the culture. 5. The method according to claim 4, characterized in that in the culture of microorganisms microorganisms of the species Methanosarcina acetivorans make up at least 10% of the total number of microorganisms present in the culture.
  • microorganisms of the species Methanosarcina acetivorans make up at least 50% of the total number of microorganisms present in the culture.
  • Biomass is continuously increased.
  • Methanosarcina acetivorans take place continuously.
  • Methanosarcina acetivorans accounts for between 10 8 % and 50% of the total number of microorganisms present in the fermentation substrate.
  • microorganism of the species Methanosarcina acetivorans is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanosarcina acetivorans between 10 "6 % and 25% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanosarcina acetivorans is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanosarcina acetivorans between 10 '4 % and 10% of the total in Fermentation substrate present microorganisms.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 97.39% sequence identity with the nucleotide sequence SEQ ID No. 16.
  • Microorganism according to claim 24 characterized in that the nucleotide sequence contains a sequence region which has more than 98% sequence identity with the nucleotide sequence SEQ ID No. 16.
  • Microorganism according to claim 26 characterized in that the nucleotide sequence contains a sequence region which has more than 99% sequence identity with the nucleotide sequence SEQ ID No. 16.
  • Microorganism according to claim 27 characterized in that the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 16.
  • Microorganism according to claim 28 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 16.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanosarcina barkeri.
  • the amount of biogas formed can be significantly increased by the addition of microorganisms of the species Methanosarcina barkeri to the fermentation substrate.
  • the addition of microorganisms of the species Methanosarcina barkeri causes a significant increase in the amount of biogas produced.
  • the amount of methane produced can also be increased by increasing the volume load, with the addition of microorganisms of the species Methanosarcina barkeri preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanosarcina barkeri therefore leads to an improvement in efficiency and efficiency of biogas plants.
  • Methanosarcina barkeri type In connection with the production of biogas by fermentation of organic substrates, microorganisms of the species Methanosarcina barkeri are not yet known.
  • a microorganism of the species Methanosarcina barkeri is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanosarcina barkeri.
  • Fermentation substrates of biogas plants were detected in microorganisms of the species Methanosarcina barkeri only in traces of less than 10 '4 % share of the total number of microorganisms present. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanosarcina barkeri can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • this type of microorganism should be present in the added culture in a concentration exceeding the natural abundance.
  • mixed cultures of any composition can be used for the addition. All that is required is that the species Methanosarcina barkeri is present in a concentration enriched with respect to the natural occurrence.
  • microorganisms of the species Methanosarcina barkeri are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanosarcina barkeri.
  • the percentage of microorganisms of the species Methanosarcina barkeri in the culture can be stated as a percentage.
  • Microorganisms of the species Methanosarcina barkeri are in a mixed culture then the predominantly present type of microorganisms, if they have the highest percentage of the different species of microorganisms present in the mixed culture.
  • microorganisms of the species Methanosarcina barkeri make at least 10 '4 %, in particular at least
  • microorganisms of the species Methanosarcina barkeri at least 10%, in particular at least 50%, particularly preferably at least 90% of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanosarcina barkeri is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanosarcina barkeri is added as a component of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanosarcina barkeri is added in an amount to the fermentation substrate, so that after addition of the proportion of the microorganism of the species Methanosarcina barkeri between 10 8 % and 50% of the total number present in the fermentation substrate
  • a microorganism of the species Methanosarcina barkeri is particularly preferably added in an amount to the fermentation substrate that makes up after addition of the content of the microorganism of the species Methanosarcina barkeri between 10 -6% and 25% of the total number present in the fermentation substrate microorganisms. More preferably, the microorganism of the species Methanosarcina barkeri is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina barkeri is between 10 '4 % and 10% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina barkeri is added in an amount to the fermentation substrate, that after addition the proportion of the microorganism of the species Methanosarcina barkeri is between 10 '3 % and 1% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone 5.5ft C140A.
  • the microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone 5.5ft C17A.
  • a microorganism of the strain Archaeon clone 5.5ft C17A is therefore preferably added.
  • the microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone 5.5ft C38A.
  • a microorganism of the strain Archaeon clone 5.5ft C38A is added.
  • the microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone ATB-KM1219.
  • a microorganism of the strain Archaeon clone ATB-KMI 219 is therefore preferably added.
  • the microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone ATB-KM1253.
  • a microorganism of the strain Archaeon clone ATB-KM '1253 is therefore preferably added.
  • the microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone MH1492 3E.
  • the present invention also encompasses the use of a microorganism of the species Methanosarcina barkeri for the fermentative production of biogas from biomass. Preference is given to the fermentative production of biogas from biomass, a microorganism of
  • Tribe tribal Archaeon clone 5.5ft C140A used. Also preferred for fermentative production of biogas from biomass is a microorganism of the strain
  • Archaeon clone 5.5ft C17A used.
  • a microorganism of the strain Archaeon clone 5.5ft C38A is used.
  • a microorganism of the strain Archaeon clone ATB-KM1219 is used.
  • a microorganism of the strain Archaeon clone ATB-KM1253 is used for the fermentative production of biogas from biomass.
  • Also preferred for fermentative production of biogas from biomass is a microorganism of the strain
  • the microorganisms Methanosarcina barkeri SBG 16 obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 13 comprises 933 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Archaeon clone 5.5ft C140A.
  • a match of 96.03% is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 96.03% sequence identity with the nucleotide sequence of SEQ ID NO: 13. More preferably, the nucleotide sequence contains a sequence range greater than 96.1% or greater than 96.2% or greater than 96.3% or greater than 96.4% or greater than 96.5% or greater than 96.6%. or more than 96.7% sequence identity with the nucleotide sequence SEQ ID NO: 13 and particularly preferably, the nucleotide sequence contains a sequence region having more than 97.0% sequence identity with the nucleotide sequence SEQ ID NO: 13.
  • the microorganism has a nucleotide sequence containing a sequence region greater than 97.5% or more
  • Nucleotide sequence SEQ ID NO. 13 and particularly preferably contains the
  • Nucleotide sequence has a sequence region that has more than 99.5% sequence identity with the
  • Nucleotide sequence SEQ ID NO. 13 has. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which is the
  • SEQ ID NO: 13 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or 23 positions or 24 positions or 25 positions or 26 positions or 27 positions or 28 positions or 29 positions or 30 positions or 31 positions or 32 positions or 33 positions or 34 positions or at 35 positions or at 36 positions or at 37 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 96.03 % Sequence identity with the nucleotide sequence SEQ ID NO: 13, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 96.1% or more 96.2% or greater than 96.3% or greater than 96.4% or greater than 96.5% or greater than 96.6% or greater than 96.7% sequence identity to the nucleotide sequence of SEQ ID NO: 13.
  • the nucleotide sequence contains a sequence region having greater than 97.0% or greater than 97.5% or greater than 98.0% or greater than 98.5% sequence identity with the nucleotide sequence of SEQ ID NO: 13.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 13 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 13.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 13.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 13 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 13 in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 13 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in one of the above - mentioned
  • microorganisms of the species Methanosarcina barkeri described method for the fermentative production of biogas from biomass In connection with microorganisms of the species Methanosarcina barkeri described method for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina barkeri SßG77 also obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 14 comprises 1 128 nucleotides.
  • the next related sequence identified was the sequence of the uncultured Archaeon clone 5.5ft C17A.
  • a 99.20% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 99.20% sequence identity with the nucleotide sequence of SEQ ID NO: 14. More preferably, the nucleotide sequence contains a sequence range greater than 99.25% or greater than 99.30% or greater than 99.35% or greater than 99.40% or greater than 99.45% or greater than 99.50%. or more than 99.55% sequence identity with the nucleotide sequence SEQ ID NO: 14, and more preferably, the nucleotide sequence contains a sequence region having more than 99.60% sequence identity with the nucleotide sequence SEQ ID NO: 14.
  • the microorganism has a nucleotide sequence which contains a sequence region which has more than 99.70% or more than 99.80% sequence identity with the nucleotide sequence SEQ ID No. 14, and more preferably the nucleotide sequence contains a sequence region which greater than 99.90% sequence identity with the nucleotide sequence SEQ ID NO: 14. According to a very particularly preferred embodiment, the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 14.
  • SEQ ID NO: 14 may be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions Nucleotide mutations are present.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence range greater than 99.20 having% sequence identity with the nucleotide sequence SEQ ID NO. 14, wherein the microorganism makes up at least 10 '4% of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.25% or greater than 99.30% or greater than 99 , 35% or more than 99.40% or more than 99.45% or more than 99.50% or more than 99.55% Sequence identity with the nucleotide sequence SEQ ID NO.
  • the nucleotide sequence contains a sequence region which has more than 99.60% sequence identity with the nucleotide sequence SEQ ID No. 14.
  • the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass comprises a microorganism having a nucleotide sequence with a sequence range greater than 99.70% or greater than 99.80% sequence identity having the nucleotide sequence SEQ ID NO: 14.
  • the nucleotide sequence most preferably contains a sequence region which has more than 99.90% sequence identity with the nucleotide sequence SEQ ID No. 14.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 14.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 14 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 14 in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 14 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina barkeri SBG18 also obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 15 comprises 1 123 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Archaeon clone 5.5ft C38A.
  • a 97.15% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having more than 97.15% sequence identity with the nucleotide sequence of SEQ ID NO: 15. More preferably, the nucleotide sequence contains a sequence range greater than 97.2% or greater than 97.3% or greater than 97.4% or greater than 97.5% or greater than 97.6% or greater than 97.7%. or more than 97.8% sequence identity with the nucleotide sequence SEQ ID NO: 15, and more preferably, the nucleotide sequence contains a sequence region having more than 97.9% sequence identity with the nucleotide sequence SEQ ID NO: 15.
  • the microorganism has a nucleotide sequence which contains a sequence region which has more than 98.0% or more than 98.5% or more than 99.0% sequence identity with the nucleotide sequence SEQ ID No. 15 and is particularly preferred
  • the nucleotide sequence contains a sequence region that has greater than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 15.
  • the nucleotide sequence contains a sequence region that corresponds to the nucleotide sequence SEQ ID No. 15.
  • SEQ ID NO: 15 can be compared to the starting nucleotide sequence at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions or at eight positions or at nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or 16 positions or 17 positions or 18 positions or 19 positions or 20 positions or 21 positions or on 22 positions or at 23 positions or at 24 positions or at 25 positions or at 26 positions or at 27 positions or at 28 positions or at 29 positions or at 30 positions or at 31 positions or at 32 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation" is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism having a nucleotide sequence containing a sequence region greater than 97.15 is present % Sequence identity with the nucleotide sequence of SEQ ID NO: 15, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 97.2% or greater than 97.3% or greater than 97 , 4% or more than 97.5% or more than 97.6% or more than 97.7% or more than 97.8% sequence identity with the nucleotide sequence SEQ ID NO: 15. More preferably, the nucleotide sequence contains a sequence region having more than 97.9% sequence identity with the nucleotide sequence SEQ ID NO: 15.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 98.0% or greater than 98.5% or greater than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 15. Most notably Preferably, the nucleotide sequence contains a sequence region having more than 99.5% sequence identity with the nucleotide sequence SEQ ID NO: 15.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 15.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 15 for fermentative
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 15 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina barkeri SBG33 also obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 30 comprises 801 nucleotides.
  • the sequence of the non-cultured krchaeon clone ATB-KM1219 was identified.
  • a match of 99.75% At a length of the particular nucleotide sequence of Methanosarcina barkeri SBG33 of 801 nucleotides and a reference sequence length of 801 Nucleotides calculated using the FASTA algorithm, a match of 99.75%.
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region greater than 99.75%.
  • nucleotide sequence SEQ ID NO. 30 Sequence identity with the nucleotide sequence SEQ ID NO. 30 has. Particularly preferably, the nucleotide sequence contains a sequence region which has more than 99.80% sequence identity with the nucleotide sequence SEQ ID No. 30 and particularly preferably contains
  • Nucleotide sequence has a sequence region that has more than 99.85% sequence identity with the nucleotide sequence SEQ ID NO: 30.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.90% sequence identity with the nucleotide sequence SEQ ID NO: 30, and more preferably the nucleotide sequence contains a sequence region having more than 99.95% sequence identity having the nucleotide sequence SEQ ID NO: 30.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 30.
  • nucleotide mutations may be present at one position or at two positions relative to the starting nucleotide sequence SEQ ID NO: 30.
  • the meaning of the term “nucleotide mutation” is explained in the “Definitions” section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 99.75 having% sequence identity with the nucleotide sequence SEQ ID NO. 30, wherein the microorganism makes up at least 10 '4% of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.80%.
  • nucleotide sequence SEQ ID NO. 30 Sequence identity with the nucleotide sequence SEQ ID NO. 30 has. Especially preferred The nucleotide sequence contains a sequence region which has more than 99.85% sequence identity with the nucleotide sequence SEQ ID No. 30.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is
  • Microorganisms present a microorganism having a nucleotide sequence with a
  • nucleotide sequence contains a
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region corresponding to the nucleotide sequence SEQ ID No. 30.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 30 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 30 in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 30 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina barkeri SBG20 also obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID NO: 17 comprises 801 nucleotides.
  • sequence of the uncultured Archaeon clone ATB-KM1253 was identified. A comparison of the sequences revealed that a total of 4 exchanges of nucleotides or deletions are present.
  • the 99.50% match is calculated using the FASTA algorithm.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 99.50% sequence identity with the nucleotide sequence of SEQ ID NO: 17. More preferably, the nucleotide sequence contains a sequence range greater than 99.55% or greater than 99.60% or greater than 99.65% or greater than 99.70% or greater than 99.75% or greater than 99.80%.
  • SEQ ID NO: 17 has sequence identity with the nucleotide sequence, and more preferably, the nucleotide sequence contains a sequence region having more than 99.85 sequence identity with the nucleotide sequence SEQ ID NO: 17.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.90% sequence identity with the nucleotide sequence SEQ ID NO: 17, and more preferably the nucleotide sequence contains a sequence region having more than 99.95% sequence identity having the nucleotide sequence of SEQ ID NO: 17.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 17.
  • nucleotide mutations may be present at one or two positions or at three positions or at four positions relative to the starting nucleotide sequence of SEQ ID NO: 17.
  • the meaning of the term “nucleotide mutation” is explained in the "Definitions” section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 99.50 % Sequence identity with the nucleotide sequence SEQ ID NO: 17, wherein the Microorganism accounts for at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.55% or greater than 99.60% or greater than 99 , 65% or more than 99.70% or more than 99.75% or more than 99.80% sequence identity with the nucleotide sequence SEQ ID NO: 17.
  • the nucleotide sequence contains a sequence region which has more than 99.85% sequence identity with the nucleotide sequence SEQ ID No. 17.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range of more than 99.90% sequence identity with the nucleotide sequence SEQ ID NO. 17 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.95% sequence identity with the nucleotide sequence SEQ ID No. 17.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 17.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 17 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 17 in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, the culture of microorganisms comprising at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 17 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina barkeri SBG24 also obtained from the fermentation substrate of a secondary fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 21 comprises 1 129 nucleotides.
  • the next related sequence identified was the sequence of the uncultivated Archaeon clone MH1492 3E.
  • Deletions are present. At a length of the particular nucleotide sequence of Methanosarcina barkeri SBG24 of 1 129 nucleotides and a length of the reference sequence of 1 129
  • the present invention also encompasses microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region greater than 98.14%.
  • sequence identity with the nucleotide sequence SEQ ID NO. 21 has. More preferably, the nucleotide sequence contains a sequence region greater than 98.2% or greater than
  • nucleotide sequence SEQ ID NO: 21 98.3% or greater than 98.4% or greater than 98.5% or greater than 98.6% sequence identity with the nucleotide sequence SEQ ID NO: 21, and most preferably the nucleotide sequence contains a sequence region greater than 98.7 % Sequence identity with the nucleotide sequence SEQ ID NO: 21, and most preferably the nucleotide sequence contains a sequence region greater than 98.7 % Sequence identity with the
  • Nucleotide sequence SEQ ID NO. 21 has.
  • the microorganism has a nucleotide sequence containing a sequence region having more than 99.0% sequence identity with the nucleotide sequence SEQ ID NO: 21, and more preferably the nucleotide sequence contains a sequence region having greater than 99.5% sequence identity having the nucleotide sequence of SEQ ID NO: 21.
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 21.
  • SEQ ID NO: 21 may be attached at one position or at two positions or at three positions or four positions or five positions or six positions or seven positions or eight positions or nine positions or ten positions or eleven positions or twelve positions or 13 positions or 14 positions or 15 positions or at 16 positions or at 17 positions or at 18 positions or at 19 positions or at 20 positions or at 21 positions nucleotide mutations.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also includes a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, wherein in the culture of microorganisms a microorganism is present, which contains a
  • Microorganism accounts for at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 98.2% or greater than 98.3% or greater than 98 , 4% or more than 98.5% sequence identity with the nucleotide sequence SEQ ID NO: 21. More preferably, the nucleotide sequence contains a sequence region having greater than 98.6% or greater than 98.7% sequence identity with the nucleotide sequence SEQ ID NO: 21.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range exceeding 99.0% sequence identity with the nucleotide sequence SEQ ID NO. 21 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 21.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 21.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 21 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 21 in a method described above in connection with microorganisms of the species Methanosarcina barkeri for the fermentative production of biogas from biomass.
  • the present invention also includes the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms at least one microorganism as above with respect to its
  • Nucleotide sequence SEQ ID NO: 21 is defined and wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • ID No. 21 has been defined and wherein the microorganism is at least 10 '4 % of the
  • Methanosarcina barkeri In connection with microorganisms of the species Methanosarcina barkeri described method for the fermentative production of biogas from biomass.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • it is a pure culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is a microorganism of the type
  • Methanosarcina barkeri is added.
  • microorganism of the species Methanosarcina barkeri is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanosarcina barkeri account for at least 10 '4 % of the total number of microorganisms present in the culture.
  • Fermentation substrate is added that after addition of the proportion of the microorganism of the species Methanosarcina barkeri constitutes at present in the fermentation substrate of microorganisms between 10 -8% and 50% of the total.
  • microorganism of the species Methanosarcina barkeri is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanosarcina barkeri between 10 '4 % and 10% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanosarcina barkeri is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanosarcina barkeri between 10 '3 % and 1% of the total in the Fermentation substrate present microorganisms.
  • Microorganism of the strain Archaeon clone ATB-KM1219 acts. 25. The method according to at least one of claims 1 to 20, characterized in that it is the microorganism of the species Methanosarcina barkeri to a microorganism of the strain Archaeon clone ATB-KM1253.
  • microorganism of the species Methanosarcina barkeri is a microorganism of the
  • microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone ATB-KM1253.
  • microorganism of the species Methanosarcina barkeri is a microorganism of the strain Archaeon clone MH1492_3E.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 96.03% sequence identity with the nucleotide sequence SEQ ID No. 13.
  • nucleotide sequence contains a sequence region which has more than 97.0% sequence identity with the nucleotide sequence SEQ ID No. 13.
  • nucleotide sequence contains a sequence region which has more than 98.0% sequence identity with the nucleotide sequence SEQ ID No. 13.
  • nucleotide sequence contains a sequence region which has more than 99.0% sequence identity with the nucleotide sequence SEQ ID No. 13.
  • nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 13.
  • Microorganism according to claim 38 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 13.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 99.20% sequence identity with the nucleotide sequence SEQ ID No. 14.
  • Microorganism according to claim 40 characterized in that the nucleotide sequence contains a sequence range exceeding 99.30%
  • nucleotide sequence contains a sequence region which has more than 99.50% sequence identity with the nucleotide sequence SEQ ID No. 14.
  • nucleotide sequence contains a sequence region which has more than 99.70% sequence identity with the nucleotide sequence SEQ ID No. 14.
  • the microorganism according to claim 43 characterized in that the nucleotide sequence contains a sequence region which has more than 99.90% sequence identity with the nucleotide sequence SEQ ID No. 14.
  • Microorganism according to claim 44 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID NO.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 97.15% sequence identity with the nucleotide sequence SEQ ID No. 15.
  • the microorganism according to claim 46 characterized in that the nucleotide sequence contains a sequence region which has more than 98.0% sequence identity with the nucleotide sequence SEQ ID No. 15.
  • nucleotide sequence contains a sequence region which has more than 98.5% sequence identity with the nucleotide sequence SEQ ID No. 15.
  • nucleotide sequence contains a sequence region which has more than 99.0% sequence identity with the nucleotide sequence SEQ ID No. 15.
  • nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 15.
  • Microorganism according to claim 50 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 15.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 99.75% sequence identity with the nucleotide sequence SEQ ID No. 30.
  • Microorganism according to claim 52 characterized in that the nucleotide sequence contains a sequence region which has more than 99.80% sequence identity with the nucleotide sequence SEQ ID No. 30.
  • microorganism according to claim 53 characterized in that the nucleotide sequence contains a sequence range which exceeds 99.85%.
  • the microorganism according to claim 54 characterized in that the nucleotide sequence contains a sequence region which has more than 99.90% sequence identity with the nucleotide sequence SEQ ID No. 30.
  • nucleotide sequence contains a sequence region which has more than 99.95% sequence identity with the nucleotide sequence SEQ ID No. 30.
  • Microorganism according to claim 56 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 30.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 99.50% sequence identity with the nucleotide sequence SEQ ID No. 17.
  • Microorganism according to claim 58 characterized in that the nucleotide sequence contains a sequence range which exceeds 99.60%
  • Microorganism according to claim 59 characterized in that the nucleotide sequence contains a sequence region which has more than 99.70% sequence identity with the nucleotide sequence SEQ ID No. 17.
  • 61 Microorganism according to claim 60, characterized in that the nucleotide sequence contains a sequence region which has more than 99.80% sequence identity with the nucleotide sequence SEQ ID No. 17.
  • nucleotide sequence contains a sequence region which has more than 99.90% sequence identity with the nucleotide sequence SEQ ID No. 17.
  • Microorganism according to claim 62 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID NO.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 98.14% sequence identity with the nucleotide sequence SEQ ID No. 21.
  • the microorganism according to claim 64 characterized in that the nucleotide sequence contains a sequence region which has more than 98.5% sequence identity with the nucleotide sequence SEQ ID No. 21.
  • nucleotide sequence contains a sequence region which has more than 99.0% sequence identity with the nucleotide sequence SEQ ID No. 21.
  • nucleotide sequence contains a sequence region which has more than 99.5% sequence identity with the nucleotide sequence SEQ ID No. 21.
  • nucleotide sequence contains a sequence region which has more than 99.9% sequence identity with the nucleotide sequence SEQ ID No. 21.
  • Microorganism according to claim 68 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 21.
  • culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass characterized in that in the culture of microorganisms, a microorganism according to claims 34 to 69 is present, wherein the microorganism at least 10 '4 % of the total in the culture microorganisms present.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass is added according to the invention a microorganism of the species Methanosarcina mazei.
  • the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanosarcina mazei causes a significant increase in the amount of biogas produced.
  • the amount of methane produced can also be increased by increasing the volume load, with the addition of microorganisms of the species Methanosarcina mazei preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanosarcina mazei therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • a microorganism of the species Methanosarcina mazei is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanosarcina mazei.
  • microorganisms of the species Methanosarcina mazei could be detected only in trace amounts of less than 10 '4% share of the total number of present microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanosarcina mazei can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • this type of microorganism should be present in the added culture in a concentration exceeding the natural abundance.
  • mixed cultures of any composition can be used for the addition. All that is required is that the species Methanosarcina mazei is present in a concentration enriched with respect to the natural occurrence.
  • the proportion of microorganisms of the species Methanosarcina mazei can be specifically identified in a mixture with the aid of fluorescence-labeled oligoprobes.
  • microorganisms of the species Methanosarcina mazei are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanosarcina mazei.
  • the proportion of microorganisms of the species Methanosarcina mazei in the culture may be expressed as a percentage. Microorganisms of the species Methanosarcina mazei are in a mixed culture then the predominant species of microorganisms, if they have the highest percentage of the different types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanosarcina mazei make at least 10 '4 %, in particular at least
  • a pure culture of a microorganism of the species Methanosarcina mazei is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanosarcina mazei is added as a constituent of at least one immobilized culture of microorganisms. Since the amount of microorganisms isolated from their natural occurrence is insufficient for the addition of the microorganisms, it is usually propagated in the form of a culture. In practice, it has been found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out in the form of an immobilized culture of microorganisms.
  • the microorganism of the species Methanosarcina mazei is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina mazei is between 10 8 % and 50% of the total number of microorganisms present in the fermentation substrate , In particular, depending on the fermenter size and thus as a function of the amount of fermentation substrate can be necessary to achieve the desired effect, an addition of very different amounts of microorganisms.
  • a microorganism of the species Methanosarcina mazei is particularly preferably added in an amount to the fermentation substrate, that after addition the content of the microorganism of the species Methanosarcina mazei between 10 -6% and 25% of the total number of in the Fermentation substrate present microorganisms. More preferably, the microorganism of the species Methanosarcina mazei is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina mazei is between 10 '4 % and 10% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina mazei is added to the fermentation substrate in an amount such that, after addition, the proportion of the microorganism of the species Methanosarcina mazei is between 10 -3 % and 1% of the total number of microorganisms present in the fermentation substrate.
  • the microorganism of the species Methanosarcina mazei is a microorganism of the strain Methanosarcina mazei Goe1.
  • a microorganism of the strain Methanosarcina mazei Goe 1 is added.
  • the present invention also encompasses the use of a microorganism of the species Methanosarcina mazei for the fermentative production of biogas from biomass.
  • a microorganism of the strain strain Methanosarcina mazei Goe1 is used for the fermentative production of biogas from biomass.
  • the microorganisms Methanosarcina mazei SBG9 obtained from the fermentation substrate of a post-fermenter were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 6 comprises 1 131 nucleotides.
  • Methanosarcina mazei Goe1 was identified as the next relative.
  • a comparison of the sequences revealed that a total of 7 exchanges of nucleotides or deletions are present.
  • the FASTA algorithm gives a 99.38% match.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 99.38% sequence identity with the nucleotide sequence of SEQ ID NO: 6. More preferably, the nucleotide sequence contains a sequence range greater than 99.40% or greater than 99.45% or greater than 99.50% or greater than 99.55% or greater than 99.60% or greater than 99.65%. Sequence identity with the nucleotide sequence SEQ ID NO. 6 and in particular Preferably, the nucleotide sequence contains a sequence region having more than 99.70% sequence identity with the nucleotide sequence SEQ ID NO: 6.
  • the microorganism has a nucleotide sequence which contains a sequence range which exceeds 99.80%.
  • nucleotide sequence SEQ ID No. 6 Sequence identity with the nucleotide sequence SEQ ID No. 6, and more preferably, the nucleotide sequence contains a sequence region having more than 99.90% sequence identity with the nucleotide sequence of SEQ ID NO: 6. According to a most preferred
  • the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 6.
  • nucleotide mutations may be present at one or two positions or at three positions or at four positions or at five positions or at six positions or at seven positions relative to the starting nucleotide sequence SEQ ID NO: 6.
  • the meaning of the term "nucleotide mutation” is explained in the "Definitions" section of the present text.
  • the present invention also encompasses a culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, in which culture of microorganisms a microorganism is present which has a nucleotide sequence containing a sequence region greater than 99.38 % Sequence identity with the nucleotide sequence SEQ ID NO: 6, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence with a sequence range greater than 99.40% or greater than 99.45% or greater than 99 , 50% or more than 99.55% or more than 99.60% or more than 99.65% sequence identity with the nucleotide sequence SEQ ID NO: 6.
  • the nucleotide sequence contains a sequence region which has more than 99.70% sequence identity with the nucleotide sequence SEQ ID No. 6.
  • the culture suitable for use in a process for the fermentative production of biogas from biomass is
  • Microorganisms present a microorganism having a nucleotide sequence with a
  • Sequence region has more than 99.80% sequence identity with the nucleotide sequence SEQ ID NO. 6 has. Most preferably, the nucleotide sequence contains a sequence region which has more than 99.90% sequence identity with the nucleotide sequence SEQ ID No. 6.
  • a microorganism in the culture of microorganisms suitable for use in a process for the fermentative production of biogas from biomass, a microorganism is present which has a nucleotide sequence which contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 6.
  • the present invention also encompasses the use of a microorganism as defined above with respect to its nucleotide sequence SEQ ID NO: 6 for the fermentative production of biogas from biomass. Preference is given to the use of a microorganism as defined above with regard to its nucleotide sequence SEQ ID No. 6 in a method described above in connection with microorganisms of the species Methanosarcina mazei for the fermentative production of biogas from biomass.
  • the present invention also encompasses the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID NO. 6 and wherein the microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture.
  • Preference is given to the use of a culture of microorganisms for the fermentative production of biogas from biomass, wherein the culture of microorganisms comprises at least one microorganism as defined above with respect to its nucleotide sequence SEQ ID No.
  • microorganism is at least 10 ' 4 % of the total number of microorganisms present in the culture, in a method described above in connection with microorganisms of the species Methanosarcina mazei for the fermentative production of biogas from biomass.
  • microorganisms make said, characterized in terms of their nucleotide sequence microorganisms at least 10 '2%, preferably to at least 1% of the total present in the culture of microorganisms.
  • the microorganisms make up at least 10%, particularly preferably at least 25%, of the total number of microorganisms present in the culture.
  • the microorganisms described make up at least 50%, in particular at least 75%, of the total number of microorganisms present in the culture.
  • the above-mentioned microorganisms make up at least 90% of the total number of microorganisms present in the culture.
  • microorganisms suitable for use in a process for the fermentative production of biogas from biomass, which is a pure culture of a microorganism, as has been characterized above with respect to its nucleotide sequence.
  • it is an immobilized culture of microorganisms.
  • the present invention includes the following aspects:
  • a process for the production of biogas from biomass in a fermentation reactor characterized in that the biomass is added to a microorganism of the species Methanosarcina mazei.
  • microorganism of the species Methanosarcina mazei is added in the form of a culture of microorganisms, wherein microorganisms of the species Methanosarcina mazei make up at least 10 '4 % of the total number of microorganisms present in the culture.
  • the method according to claim 5 characterized in that make up in the culture of microorganisms of the species Methanosarcina mazei at least 50% of the total number of microorganisms present in the culture. 7. The method according to claim 6, characterized in that constitute in the culture of microorganisms of the species Methanosarcina mazei at least 75% of the total number of microorganisms present in the culture.
  • microorganism of the species Methanosarcina mazei is added in an amount to the fermentation substrate that after addition of the proportion of the microorganism of the species Methanosarcina mazei between 10 '8 % and 50% the total number of microorganisms present in the fermentation substrate.
  • microorganism of the species Methanosarcina mazei is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanosarcina mazei between 10 "6 % and 25% of the total in Fermentation substrate present microorganisms.
  • microorganism of the species Methanosarcina mazei is added in an amount to the fermentation substrate, that after addition of the proportion of the microorganism of the species Methanosarcina mazei between 10 ⁇ 4 % and 10% of the total in Fermentation substrate present microorganisms.
  • Microorganism of the strain Methanosarcina mazei Goe1 acts.
  • a microorganism comprising a nucleic acid having a nucleotide sequence, characterized in that the nucleotide sequence contains a sequence region which has more than 99.38% sequence identity with the nucleotide sequence SEQ ID No. 6.
  • Microorganism according to claim 24 characterized in that the nucleotide sequence contains a sequence region which has more than 99.40% sequence identity with the nucleotide sequence SEQ ID No. 6.
  • Microorganism according to claim 26 characterized in that the nucleotide sequence contains a sequence region which has more than 99.80% sequence identity with the nucleotide sequence SEQ ID No. 6.
  • Microorganism according to claim 27 characterized in that the nucleotide sequence contains a sequence region which has more than 99.90% sequence identity with the nucleotide sequence SEQ ID No. 6.
  • Microorganism according to claim 28 characterized in that the nucleotide sequence contains a sequence region which corresponds to the nucleotide sequence SEQ ID No. 6.
  • Microorganisms a microorganism according to claims 24 to 29 is present, wherein the microorganism constitutes at least 10 '4 % of the total number of microorganisms present in the culture.
  • the present invention provides a method for producing biogas from biomass in a fermentation reactor.
  • the biomass according to the invention is added to a microorganism of the species Methanothermobacter wolfeii.
  • the amount of biogas formed can be significantly increased.
  • the addition of microorganisms of the species Methanothermobacter wolfeii causes a significant increase in the amount of biogas produced.
  • the amount of methane formed can also be increased by increasing the space load, with the addition of microorganisms of the species Methanothermobacter wolfeii preventing the fermentation process from becoming unstable under the altered conditions.
  • the use of microorganisms of the species Methanothermobacter wolfeii therefore leads to an improvement in the efficiency and efficiency of biogas plants.
  • Methanothermobacter wolfeii also the synonyms "Methanothermobacter wolfef as well as
  • a microorganism of the species Methanothermobacter wolfeii is added in the form of a culture of microorganisms consisting predominantly of microorganisms of the species Methanothermobacter wolfeii.
  • microorganisms of the species could be detected from Methanothermobacter present microorganisms wolfeii only in trace amounts of less than 10 '4% share of the total number.
  • the amount of microorganisms isolated from their natural occurrence is insufficient, usually a propagation is carried out in the form of a culture.
  • it is found that the addition of the microorganisms to the fermentation substrate of a fermenter is most easily carried out directly in the form of a culture of microorganisms.
  • Methanothermobacter wolfeii can be carried out in the form of a culture suspension or in the form of dry, freeze-dried or moist cell pellets.
  • microorganisms of the species Methanothermobacter wolfeii are preferably added to the fermentation substrate in the form of cultures of microorganisms, the cultures of microorganisms consisting predominantly of microorganisms of the species Methanothermobacter wolfeii.
  • the proportion of microorganisms of the species Methanothermobacter wolfeii in the culture can be stated in percent.
  • Microorganisms of the species Methanothermobacter wolfeii are in a mixed culture then the predominantly present type of microorganisms, if they have the highest percentage of the various types of microorganisms present in the mixed culture.
  • microorganisms of the species Methanothermobacter wolfeii at least 10 '4%, especially at least 10 "2%, more preferably at least 1% of the total number of the added to the fermentation substrate culture microorganisms present. According to further preferred embodiments make microorganisms of the species Methanothermobacter wolfeii at least 10%, in particular at least 50%, more preferably at least 90% of the total number of microorganisms present in the culture.
  • a pure culture of a microorganism of the species Methanothermobacter wolfeii is added. Due to the specific metabolic processes and activities, the addition of a pure culture can especially contribute to an improved methane production.
  • a microorganism of the species Methanothermobacter wolfeii is added as a component of at least one immobilized culture of microorganisms.
  • Microorganisms the amount of isolated from their natural occurrence
  • Microorganisms is insufficient, is usually made an increase in the form of a culture.
  • the addition of the microorganisms to the fermentation substrate of a fermenter is most easily achieved in the form of an immobilized culture of
  • Microorganisms is made.
  • the microorganism of the species Methanothermobacter wolfeii is added in an amount to the fermentation substrate, so that after addition of the proportion of the microorganism of the species Methanothermobacter wolfeii between 10 '8 % and 50% of the total number of present in the fermentation substrate microorganisms accounts.
  • an addition of very different amounts of microorganisms depending on the fermenter size and thus as a function of the amount of fermentation substrate can be necessary to achieve the desired effect, an addition of very different amounts of microorganisms.
  • a microorganism of the species Methanothermobacter is particularly preferred wolfeii in an amount added to the fermentation substrate that constitutes wolfeii after addition of the content of the microorganism of the species Methanothermobacter between 10 -6% and 25% of the total number present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanothermobacter is particularly preferred wolfeii in an amount added to the fermentation substrate that constitutes wolfeii after addition of the content of the microorganism of the species Methanothermobacter between 10 -4% and 10% of the total number present in the fermentation substrate microorganisms.
  • the microorganism of the species Methanothermobacter wolfeii is added in an amount to the fermentation substrate such that, after addition, the proportion of the microorganism of the species Methanothermobacter wolfeii is between 10 '. 3 % and 1% of the total number of microorganisms present in the fermentation substrate.
  • the present invention also encompasses the use of a microorganism of the species Methanothermobacter wolfeii for the fermentative production of biogas from biomass.
  • the microorganisms Methanothermobacter wolfeii SBG10 obtained from the fermentation substrate of a postgrader were subjected to a sequence analysis.
  • the determined 16S rRNA sequence SEQ ID No. 7 comprises 1130 nucleotides.
  • Methanothermobacter wolfeii was identified as the next relative.
  • the FASTA algorithm gives a 99.56% match.
  • the present invention also includes microorganisms having a nucleic acid having a nucleotide sequence containing a sequence region having greater than 99.56% sequence identity with the nucleotide sequence of SEQ ID NO: 7. More preferably, the nucleotide sequence contains a sequence range greater than 99.58% or greater than 99.60% or greater than 99.62% or greater than 99.64% or greater than 99.66% or greater than 99.68%. Sequence identity with the nucleotide sequence SEQ ID NO. 7, and more preferably, the nucleotide sequence contains a sequence region having more than 99.70% sequence identity with the nucleotide sequence SEQ ID NO: 7.

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Abstract

L'invention concerne un procédé de génération de biogaz à partir de biomasse dans un réacteur de fermentation, un micro-organisme du type Methanoculleus bourgensis étant ajouté à la biomasse.
PCT/DE2010/075035 2009-04-11 2010-04-08 Micro-organismes méthanogènes permettant de générer du biogaz Ceased WO2010115424A1 (fr)

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