[go: up one dir, main page]

WO2010039180A3 - Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique - Google Patents

Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique Download PDF

Info

Publication number
WO2010039180A3
WO2010039180A3 PCT/US2009/005250 US2009005250W WO2010039180A3 WO 2010039180 A3 WO2010039180 A3 WO 2010039180A3 US 2009005250 W US2009005250 W US 2009005250W WO 2010039180 A3 WO2010039180 A3 WO 2010039180A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte molecules
substrate
particles
fluid sample
labeling agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2009/005250
Other languages
English (en)
Other versions
WO2010039180A2 (fr
Inventor
David C. Duffy
David M. Rissin
Linan Song
David R. Walt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Quanterix Corp
Original Assignee
Quanterix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quanterix Corp filed Critical Quanterix Corp
Priority to EP09736701A priority Critical patent/EP2352842A2/fr
Publication of WO2010039180A2 publication Critical patent/WO2010039180A2/fr
Publication of WO2010039180A3 publication Critical patent/WO2010039180A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des procédés, des systèmes et des trousses pour détecter, quantifier et/ou analyser un échantillon fluidique comprenant des molécules ou des particules d'analyte en faible concentration. Dans certains modes de la réalisation de l'invention, les procédés pour détecter et/ou pour quantifier les molécules d'analyte dans un échantillon consistent à capturer plusieurs molécules d'analyte sur un substrat (par exemple, un réseau comprenant plusieurs contenants de réaction). Le substrat peut ensuite être exposé à des composés réactionnels supplémentaires comme au moins un ligand de liaison. Le substrat peut éventuellement être également exposé à une molécule d'agent de marquage précurseur, cette molécule étant, dans certains cas, convertie en une molécule d'agent de marquage qui peut être détectée soit directement, soit indirectement, cette détermination pouvant être liée à la présence des molécules d'analyte et/ou pouvant être utilisée pour quantifier lesdites molécules d'analyte. Bien que des aspects variés de l'invention peuvent faire intervenir un certain nombre de formats d'épreuves différents, dans un mode de réalisation, les épreuves biologiques sont réalisées dans plusieurs contenants de réaction définis, au moins en partie, par les extrémités distales de brins de fibre optique.
PCT/US2009/005250 2008-09-23 2009-09-22 Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique Ceased WO2010039180A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP09736701A EP2352842A2 (fr) 2008-09-23 2009-09-22 Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/236,484 US20100075862A1 (en) 2008-09-23 2008-09-23 High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US12/236,484 2008-09-23

Publications (2)

Publication Number Publication Date
WO2010039180A2 WO2010039180A2 (fr) 2010-04-08
WO2010039180A3 true WO2010039180A3 (fr) 2010-05-27

Family

ID=41404461

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/005250 Ceased WO2010039180A2 (fr) 2008-09-23 2009-09-22 Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique

Country Status (3)

Country Link
US (1) US20100075862A1 (fr)
EP (1) EP2352842A2 (fr)
WO (1) WO2010039180A2 (fr)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8460879B2 (en) * 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
CA2734029C (fr) 2007-08-30 2016-03-29 The Trustees Of Tufts College Procedes pour determiner la concentration d'un analyte dans une solution
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US20100075439A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification
US8415171B2 (en) * 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) * 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
JP5363663B2 (ja) 2010-03-01 2013-12-11 クワンテリクス コーポレーション 分子または粒子を検出するアッセイにおけるダイナミックレンジを拡張するための方法またはシステム
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US20140302532A1 (en) * 2011-04-12 2014-10-09 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury
WO2013148498A1 (fr) * 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Conjugués de signalisation, et procédés d'utilisation
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
HK1213019A1 (zh) 2013-03-13 2016-06-24 Meso Scale Technologies, Llc 改良的測定方法
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CA2904326C (fr) 2013-03-15 2021-05-11 Lubrizol Advanced Materials, Inc. Compositions cpvc exemptes de metaux lourds
US10408823B2 (en) 2014-05-15 2019-09-10 Meso Scale Technologies, Llc. Assay methods
WO2016130923A1 (fr) 2015-02-13 2016-08-18 Quanterix Corporation Dosages immunologiques pour la détection différentielle de clostridium difficile
CN107430121B (zh) * 2015-03-13 2020-06-23 希森美康株式会社 受试物质的检测方法及在该方法中使用的试剂盒
DK3450980T3 (da) 2015-10-07 2021-06-07 Selma Diagnostics Aps Fremgangsmåde til optagelse af et stabilt dråbemønster
WO2017096114A1 (fr) * 2015-12-04 2017-06-08 Daktari Diagnostics, Inc. Procédés de détermination d'analytes dans des fluides
EP3491385B1 (fr) 2016-07-29 2021-04-07 Selma Diagnostics ApS Améliorations apportées à des procédés de comptage numérique
US12130290B2 (en) 2016-11-29 2024-10-29 Trustees Of Tufts College Compositions and methods for diagnosing breast cancer
WO2018119401A2 (fr) * 2016-12-22 2018-06-28 Daktari Diagnostics, Inc. Dispositifs et procédés pour déterminer un ou plusieurs analytes dans des fluides
US11391735B2 (en) 2017-09-06 2022-07-19 AimPlex Biosciences, Inc. Methods for improving the dynamic range of biological assays
WO2019060607A1 (fr) 2017-09-20 2019-03-28 Quanterix Corporation Procédés et systèmes de détection de molécules d'analytes
WO2020180645A1 (fr) 2019-03-01 2020-09-10 Meso Scale Technologies, Llc. Sondes marquées par électrochimioluminescence destinées à être utilisées dans des procédés de dosage immunologique, procédés utilisant ces sondes et kits les comprenant
EP3973533A1 (fr) * 2019-05-22 2022-03-30 Biomerieux Procédés et systèmes de fabrication d'un réacteur de dosage de production
WO2021030303A1 (fr) 2019-08-12 2021-02-18 Voyager Therapeutics, Inc. Dosage immunologique à haute sensibilité pour la détection de la frataxine dans des liquides biologiques
CA3172579A1 (fr) 2020-04-15 2021-10-21 David C. Duffy Procedes et systemes lies a des dosages hautement sensibles et a l'administration d'objets de capture
US11686730B2 (en) 2020-04-30 2023-06-27 Quanterix Corporation Quantitative antibody test
EP4157992A4 (fr) 2020-05-26 2024-07-31 Bio-Rad Laboratories, Inc. Appareil et procédé de détection numérique rapide

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007098148A2 (fr) * 2006-02-21 2007-08-30 Trustees Of Tufts College Procédés et réseaux pour la détection d'analytes cibles et la détermination de la concentration d'analytes cibles en solution

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3212986A (en) * 1964-11-05 1965-10-19 Robert T Pennington Three tank separate superheat reactor
SE427505B (sv) * 1977-03-04 1983-04-11 Pharmacia Diagnostics Ab Reagens till anvendning vid immunkemiska bestemningsmetoder
US4200110A (en) * 1977-11-28 1980-04-29 United States Of America Fiber optic pH probe
FR2565350B1 (fr) * 1984-06-05 1986-10-10 Paris Nord Universite Moyens propres a permettre le support, le traitement, le stockage et l'analyse automatiques en continu d'echantillons biologiques
US4631211A (en) * 1985-03-25 1986-12-23 Scripps Clinic & Research Foundation Means for sequential solid phase organic synthesis and methods using the same
US4780421A (en) * 1986-04-03 1988-10-25 Sclavo Inc. Cleavable labels for use in binding assays
US5252494A (en) * 1986-06-25 1993-10-12 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix
FR2613074B1 (fr) * 1987-03-27 1990-06-08 Commissariat Energie Atomique Capteur chimique actif, a fibres optiques
US4962037A (en) * 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US4924870A (en) * 1989-01-13 1990-05-15 Fiberoptic Sensor Technologies, Inc. Fiber optic sensors
US5026159A (en) * 1989-05-19 1991-06-25 Acrogen, Inc. Area-modulated luminescence (AML)
US5190857A (en) * 1989-05-19 1993-03-02 Acrogen, Inc. Optical method for measuring an analyte using area-modulated luminescence
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5152816A (en) * 1989-10-16 1992-10-06 Corning Incorporated Method of enlarging end of capillary tube bore
US5108961A (en) * 1989-12-29 1992-04-28 Circon Corporation Etchable core glass compositions and method for manufacturing a high performance microchannel plate
US5532138A (en) * 1990-04-26 1996-07-02 Behringwerke Ag Method and kits for determining peroxidatively active catalysts
US6582908B2 (en) * 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
US5320814A (en) * 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5250264A (en) * 1991-01-25 1993-10-05 Trustees Of Tufts College Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
US5244813A (en) * 1991-01-25 1993-09-14 Trustees Of Tufts College Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample
US5244636A (en) * 1991-01-25 1993-09-14 Trustees Of Tufts College Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample
US6943034B1 (en) * 1991-11-22 2005-09-13 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
US5315375A (en) * 1992-02-11 1994-05-24 Acrogen, Inc. Sensitive light detection system
US5329461A (en) * 1992-07-23 1994-07-12 Acrogen, Inc. Digital analyte detection system
US5298741A (en) * 1993-01-13 1994-03-29 Trustees Of Tufts College Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample
US7241569B2 (en) * 1993-01-18 2007-07-10 Olympus Corporation Method and a device for the evaluation of biopolymer fitness
JP3441763B2 (ja) * 1993-06-30 2003-09-02 キリン−アムジエン・インコーポレーテツド モノクローナル抗体及び該モノクローナル抗体を用いたヒトｇ−ｃｓｆの測定法
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5512490A (en) * 1994-08-11 1996-04-30 Trustees Of Tufts College Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
US6013445A (en) * 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5532379A (en) * 1995-05-05 1996-07-02 Pierce Chemical Company Biotin containing heterobifunctional cleavable compounds
US5690894A (en) * 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5545531A (en) * 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
US5633972A (en) * 1995-11-29 1997-05-27 Trustees Of Tufts College Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
US5814524A (en) * 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
US6387707B1 (en) * 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
US6133436A (en) * 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
AU5794498A (en) * 1996-12-10 1998-07-03 Genetrace Systems, Inc. Releasable nonvolatile mass-label molecules
SE9700384D0 (sv) * 1997-02-04 1997-02-04 Biacore Ab Analytical method and apparatus
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US7622294B2 (en) * 1997-03-14 2009-11-24 Trustees Of Tufts College Methods for detecting target analytes and enzymatic reactions
US20030027126A1 (en) * 1997-03-14 2003-02-06 Walt David R. Methods for detecting target analytes and enzymatic reactions
US6023540A (en) * 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6406845B1 (en) * 1997-05-05 2002-06-18 Trustees Of Tuft College Fiber optic biosensor for selectively detecting oligonucleotide species in a mixed fluid sample
US6203983B1 (en) * 1997-06-16 2001-03-20 Affymetrix, Inc. Method for detecting chemical interactions between naturally occurring bio-polymers which are non-identical binding partners
FR2764989B1 (fr) * 1997-06-20 1999-08-27 Pasteur Sanofi Diagnostics Procede de dosage du c-peptide
US6174695B1 (en) * 1997-08-12 2001-01-16 The Regents Of The University Of California Epoxide hydrolase inhibitor methods
US5987180A (en) * 1997-09-26 1999-11-16 Sarnoff Corporation Multiple component compression encoder motion search method and apparatus
US7115884B1 (en) * 1997-10-06 2006-10-03 Trustees Of Tufts College Self-encoding fiber optic sensor
US7348181B2 (en) * 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
SE9800360D0 (sv) * 1998-02-06 1998-02-06 Goeteborg University Science I Method, apparatus and flow cell for high sensitivity detection of fluorescent molecules
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
US6285807B1 (en) * 1998-11-16 2001-09-04 Trustees Of Tufts College Fiber optic sensor for long-term analyte measurements in fluids
US6429027B1 (en) * 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
EP1190100B1 (fr) * 1999-05-20 2012-07-25 Illumina, Inc. Decodage combinatoire de jeux d'acides nucleiques aleatoires
US6713309B1 (en) * 1999-07-30 2004-03-30 Large Scale Proteomics Corporation Microarrays and their manufacture
US7244559B2 (en) * 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7211390B2 (en) * 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001055703A1 (fr) * 2000-01-31 2001-08-02 Board Of Regents, The University Of Texas System Procede de preparation d'un reseau de capteurs
AU2001247417A1 (en) * 2000-03-14 2001-09-24 Trustees Of Tufts College Cross-reactive sensors
US20040101918A1 (en) * 2001-02-15 2004-05-27 Sabina Cauci Diagnostic method for bacterial vaginosis based on detecting antibodies against gvh toxin of gardnerella vaginalis
EP1421365A1 (fr) * 2001-07-19 2004-05-26 Tufts University Dispositif de reseau optique et procedes d'utilisation de ce dispositif pour le criblage, l'analyse et la manipulation de particules
AU2002353049A1 (en) * 2001-12-14 2003-06-30 Corning Incorporated Two step etching process for an optical fiber preform
US20030198573A1 (en) * 2002-01-25 2003-10-23 Illumina, Inc. Sensor arrays for detecting analytes in fluids
WO2003071223A2 (fr) * 2002-02-19 2003-08-28 Tufts University Mesure / stimulation electro-optique
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7387889B2 (en) * 2002-08-22 2008-06-17 Massachusetts Institute Of Technology Measurement of concentrations and binding energetics
ES2342665T3 (es) * 2003-01-29 2010-07-12 454 Corporation Secuenciacion desde dos extremos.
WO2004107321A2 (fr) * 2003-05-28 2004-12-09 Tufts University Stockage de donnees optique a densite elevee
SE0302525D0 (sv) * 2003-09-24 2003-09-24 Biacore Ab Method and system for interaction analysis
NZ547494A (en) * 2003-10-28 2008-05-30 Bioarray Solutions Ltd Gel-shell beads with absorbed or bound biomolecules
EP1805500A4 (fr) * 2004-09-28 2008-05-07 Singulex Inc Systeme et methode d'analyse d'echantillons
WO2006047038A1 (fr) * 2004-09-28 2006-05-04 Trustees Of Tufts College Appareil et procede d'analyse de la migration cellulaire
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US7602952B2 (en) * 2004-11-16 2009-10-13 Illumina, Inc. Scanner having spatial light modulator
US7595473B2 (en) * 2005-08-22 2009-09-29 Tufts University Method and system of array imaging
US20070074972A1 (en) * 2005-09-13 2007-04-05 Fluidigm Corporation Microfluidic assay devices and methods
US20070116607A1 (en) * 2005-11-23 2007-05-24 Pharmacom Microlelectronics, Inc. Microsystems that integrate three-dimensional microarray and multi-layer microfluidics for combinatorial detection of bioagent at single molecule level
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
WO2009012340A2 (fr) * 2007-07-16 2009-01-22 California Institute Of Technology Dispositifs microfluidiques, procédés et systèmes pour détecter des molécules cibles
US8093063B2 (en) * 2007-11-29 2012-01-10 Quest Diagnostics Investments Incorporated Assay for detecting genetic abnormalities in genomic nucleic acids
US20090254180A1 (en) * 2008-01-14 2009-10-08 Walt Pazanowski Customizable vertebral replacement assembly system
US9487822B2 (en) * 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
US20090289834A1 (en) * 2008-05-23 2009-11-26 Honeywell International Inc. High integrity radio altimeter
US20100075439A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of molecules by capture-and-release using reducing agents followed by quantification
US8222047B2 (en) * 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US20100075355A1 (en) * 2008-09-23 2010-03-25 Quanterix Corporation Ultra-sensitive detection of enzymes by capture-and-release followed by quantification

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007098148A2 (fr) * 2006-02-21 2007-08-30 Trustees Of Tufts College Procédés et réseaux pour la détection d'analytes cibles et la détermination de la concentration d'analytes cibles en solution

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"Chapter 10: Enzyme substrates ED - HAUGLAND R P", 1 January 2005, HANDOOK : A GUIDE TO FLUORESCENT PROBES AND LABELING TECHNOLOGIES. INVITROGEN, EUGENE, OR : MOLECULAR PROBES, US, PAGE(S) 473 - 538, ISBN: 9780971063648, XP001536718 *
LI ZHAOHUI ET AL: "Detection of single-molecule DNA hybridization using enzymatic amplification in an array of femtoliter-sized reaction vessels.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 24 SEP 2008, vol. 130, no. 38, 3 September 2008 (2008-09-03), pages 12622 - 12623, XP002564820, ISSN: 1520-5126 *
RISSIN DAVID M ET AL: "Digital concentration readout of single enzyme molecules using femtoliter arrays and Poisson statistics", NANO LETTERS, ACS, WASHINGTON, DC, US, vol. 6, no. 3, 1 March 2006 (2006-03-01), pages 520 - 523, XP002483266, ISSN: 1530-6984, [retrieved on 20060221] *
RISSIN DAVID M ET AL: "Digital readout of target binding with attomole detection limits via enzyme amplification in femtoliter arrays", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, NEW YORK, USA, vol. 128, no. 19, 17 May 2006 (2006-05-17), pages 6286 - 6287, XP002483267, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
US20100075862A1 (en) 2010-03-25
WO2010039180A2 (fr) 2010-04-08
EP2352842A2 (fr) 2011-08-10

Similar Documents

Publication Publication Date Title
WO2010039180A3 (fr) Détermination ultra-sensible de la concentration de molécules ou de particules d'analyte dans un échantillon fluidique
WO2011109364A3 (fr) Détection ultrasensible de molécules ou de particules à l'aide de billes ou autres objets de capture
Cennamo et al. An easy way to realize SPR aptasensor: A multimode plastic optical fiber platform for cancer biomarkers detection
IN2014DN08220A (fr)
BRPI0809138B8 (pt) método para detectar a presença ou quantidade de um ou mais analitos em uma amostra biológica
BRPI0804853A2 (pt) dispositivo de ensaio para determinar a presença e/ou extensão de um ou mais analitos em uma amostra de lìquido, e, método de realização de um ensaio para determinar a presença e/ou extensão de um analito em uma amostra de lìquido
WO2007084886A3 (fr) Analyses multiplexees d’echantillons test
SE0800871L (sv) Analysförfarande och anordning
WO2008057781A3 (fr) Appareil portable pour analyse d'échantillon améliorée
WO2008008785A3 (fr) Détection ultra-sensible d'analytes
CA2863353C (fr) Dispositifs, methodes et kits de test pour dosage d'analyte electronique
WO2009013658A3 (fr) Procédés et systèmes microfluidiques destinés à être utilisés dans la détection de substances à analyser
EP2340301A4 (fr) Analyseur d imagerie pour essai d analytes
WO2010053980A3 (fr) Essai d'intégrité d'adn pour diagnostic de cancer, utilisant la spectroscopie de fluorescence confocale
MX2011010826A (es) Dispositivos para diagnostico y métodos relacionados.
WO2014011261A3 (fr) Système et procédé de détection optique basés sur l'action capillaire
WO2006080024A3 (fr) Dispositif, systeme et procede d'analyse in vivo
WO2007005666A3 (fr) Systeme et methode de detection d'analytes au moyen de recepteurs differentiels
CN104212803A (zh) 用于汞离子定量快速检测的核酸适配体序列及其检测方法
WO2004102170A8 (fr) Biopuce a reseau de fibres optiques basee sur la spectroscopie a interferences par reflectometrie en lumiere blanche
WO2012145270A3 (fr) Amplification cyclique de polymère luminescent
Xu et al. based chiral biosensors using enzyme encapsulation in hydrogel network for point-of-care detection of lactate enantiomers
WO2007029164A3 (fr) Detection d'oxyde nitrique
WO2005073405A3 (fr) Polynucleotide catalytique et utilisation de celui-ci pour determiner des analytes
CA2483757A1 (fr) Temoin interne pour elements d'analyse

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 09736701

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2009736701

Country of ref document: EP