WO2010037397A1

WO2010037397A1 - Mhc multimers in cmv immune monitoring

Info

Publication number: WO2010037397A1
Application number: PCT/DK2009/050257
Authority: WO
Inventors: Liselotte Brix; Jørgen SCHØLLER; Henrik Pedersen
Original assignee: Dako Denmark ApS
Current assignee: Dako Denmark ApS
Priority date: 2008-10-01
Filing date: 2009-10-01
Publication date: 2010-04-08
Anticipated expiration: 2011-04-01

Abstract

The present invention relates to MHC-peptide complexes and uses thereof in the diagnosis of, treatment of or vaccination against a disease in an individual. More specifically the invention discloses MHC complexes comprising CMV antigenic peptides and uses there of.

Description

MHC MULTIMERS IN CMV IMMUNE MONITORING

All patent and non-patent references cited in US 61/101 ,898 as well as in this application are hereby incorporated by reference in their entirety. US 61/101 ,898 is hereby also incorporated herein by reference in its entirety.

PCT/DK2009/050185, PCT/DK2008/0001 18, and PCT/DK2008/050167 are hereby also incorporated herein by reference in their entirety. All patent and non-patent references cited in PCT/DK2009/050185, PCT/DK2008/0001 18, and PCT/DK2008/050167 are hereby also incorporated by reference in their entirety.

Field of invention

The present invention relates to MHC-peptide complexes and uses thereof in the treatment of a disease in an individual.

Background of invention

Biochemical interactions between peptide epitope specific membrane molecules encoded by the Major Histocompatibility Complex (MHC, in humans HLA) and T-cell receptors (TCR) are required to elicit specific immune responses. This requires activation of T-cells by presentation to the T-cells of peptides against which a T-cell response should be raised. The peptides are presented to the T-cells by the MHC complexes.

The immune response

The immune response is divided into two parts termed the innate immune response and the adaptive immune response. Both responses work together to eliminate pathogens (antigens). Innate immunity is present at all times and is the first line of defense against invading pathogens. The immediate response by means of pre- existing elements, i.e. various proteins and phagocytic cells that recognize conserved features on the pathogens, is important in clearing and control of spreading of pathogens. If a pathogen is persistent in the body and thus only partially cleared by the actions of the innate immune system, the adaptive immune system initiate a response against the pathogen. The adaptive immune system is capable of eliciting a response against virtually any type of pathogen and is unlike the innate immune system capable of establishing immunological memory.

The adaptive response is highly specific to the particular pathogen that activated it but it is not so quickly launched as the innate when first encountering a pathogen.

However, due to the generation of memory cells, a fast and more efficient response is generated upon repeated exposure to the same pathogen. The adaptive response is carried out by two distinct sets of lymphocytes, the B cells producing antibodies leading to the humoral or antibody mediated immune response, and the T cells leading to the cell mediated immune response.

T cells express a clonotypic T cell receptor (TCR) on the surface. This receptor enable the T cell to recognize peptide antigens bound to major histocompatibility complex (MHC) molecules, called human leukocyte antigens (HLA) in man. Depending on the type of pathogen, being intracellular or extracellular, the antigenic peptides are bound to MHC class I or MHC class II, respectively. The two classes of MHC complexes are recognized by different subsets of T cells; Cytotoxic CD8+ T cells recognizing MHC class I and CD4+ helper cells recognizing MHC class II. In general, TCR recognition of MHC-peptide complexes result in T cell activation, clonal expansion and differentiation of the T cells into effector, memory and regulatory T cells.

B cells express a membrane bound form of immunoglobulin (Ig) called the B cell receptor (BCR). The BCR recognizes an epitope that is part of an intact three dimensional antigenic molecule. Upon BCR recognition of an antigen the BCR:antigen complex is internalized and fragments from the internalized antigen is presented in the context of MHC class Il on the surface of the B cell to CD4+ helper T-cells (Th). The specific Th cell will then activate the B cell leading to differentiation into an antibody producing plasma cell.

A very important feature of the adaptive immune system is its ability to distinguish between self and non-self antigens, and preferably respond against non-self. If the immune system fails to discriminate between the two, specific immune responses against self-antigens are generated. These autoimmune reactions can lead to damage of self-tissue. The adaptive immune response is initiated when antigens are taken up by professional antigen presenting cells such as dendritic cells, Macrophages, Langerhans cells and B- cells. These cells present peptide fragments, resulting from the degradation of proteins, in the context of MHC class Il proteins (Major Histocompatibility Complex) to helper T cells. The T helper cells then mediate help to B-cells and antigen-specific cytotoxic T cells, both of which have received primary activation signals via their BCR respective TCR. The help from the Th-cell is mediated by means of soluble mediators e.g. cytokines.

In general the interactions between the various cells of the cellular immune response is governed by receptor-ligand interactions directly between the cells and by production of various soluble reporter substances e.g. cytokines by activated cells.

MHC-peptide complexes. MHC complexes function as antigenic peptide receptors, collecting peptides inside the cell and transporting them to the cell surface, where the MHC-peptide complex can be recognized by T-lymphocytes. Two classes of classical MHC complexes exist, MHC class I and II. The most important difference between these two molecules lies in the protein source from which they obtain their associated peptides. MHC class I molecules present peptides derived from endogenous antigens degraded in the cytosol and are thus able to display fragments of viral proteins and unique proteins derived from cancerous cells. Almost all nucleated cells express MHC class I on their surface even though the expression level varies among different cell types. MHC class Il molecules bind peptides derived from exogenous antigens. Exogenous proteins enter the cells by endocytosis or phagocytosis, and these proteins are degraded by proteases in acidified intracellular vesicles before presentation by MHC class Il molecules. MHC class Il molecules are only expressed on professional antigen presenting cells like B cells and macrophages.

The three-dimensional structure of MHC class I and Il molecules are very similar but important differences exist. MHC class I molecules consist of two polypeptide chains, a heavy chain, α, spanning the membrane and a light chain, β2-microglobulin (β2m). The heavy chain is encoded in the gene complex termed the major histocompatibility complex (MHC), and its extracellular portion comprises three domains, α1 , α2 and α3. The β2m chain is not encoded in the MHC gene and consists of a single domain, which together with the α3 domain of the heavy chain make up a folded structure that closely resembles that of the immunoglobulin. The α1 and α2 domains pair to form the peptide binding cleft, consisting of two segmented α helices lying on a sheet of eight β-strands. In humans as well as in mice three different types of MHC class I molecule exist. HLA- A, B, C are found in humans while MHC class I molecules in mice are designated H- 2K, H-2D and H-2L

The MHC class Il molecule is composed of two membrane spanning polypeptide chains, α and β, of similar size (about 30000 Da). Genes located in the major histocompatibility complex encode both chains. Each chain consists of two domains, where α1 and β1 forms a 9-pocket peptide-binding cleft, where pocket 1 , 4, 6 and 9 are considered as major peptide binding pockets. The α2 and β2, like the α2 and β2m in the MHC class I molecules, have amino acid sequence and structural similarities to immunoglobulin constant domains. In contrast to MHC class I complexes, where the ends of the antigenic peptide is buried, peptide-ends in MHC class Il complexes are not. HLA-DR, DQ and DP are the human class Il molecules, H-2A, M and E are those of the mice.

A remarkable feature of MHC genes is their polymorphism accomplished by multiple alleles at each gene. The polygenic and polymorphic nature of MHC genes is reflected in the peptide-binding cleft so that different MHC complexes bind different sets of peptides. The variable amino acids in the peptide binding cleft form pockets where the amino acid side chains of the bound peptide can be buried. This permits a specific variant of MHC to bind some peptides better than others.

MHC multimers

Due to the short half-life of the peptide-MHC-T cell receptor ternary complex (typically between 10 and 25 seconds) it is difficult to label specific T cells with labelled MHC- peptide complexes, and like-wise, it is difficult to employ such monomers of MHC- peptide for therapeutic and vaccine purposes because of their weak binding. In order to circumvent this problem, MHC multimers have been developed. These are complexes that include multiple copies of MHC-peptide complexes, providing these complexes with an increased affinity and half-life of interaction, compared to that of the monomer MHC-peptide complex. The multiple copies of MHC-peptide complexes are attached, covalently or non-covalently, to a multimerization domain. Known examples of such MHC multimers include the following:

• MHC-dimers: Each MHC dimer contains two copies of MHC-peptide. IgG is used as multimerization domain, and one of the domains of the MHC protein is covalently linked to IgG.

• MHC-tetramers: Each MHC-tetramer contains four copies of MHC-peptide, each of which is biotinylated. The MHC complexes are held together in a complex by the streptavidin tetramer protein, providing a non-covalent linkage between a streptavidin monomer and the MHC protein. Tetramers are described in US patent 5,635,363.

• MHC pentamers: Five copies of MHC-peptide complexes are multimerised by a self-assembling coiled-coil domain, to form a MHC pentamer. MHC pentamers are described in the US patent 2004209295

• MHC dextramers: A large number of MHC-peptide complexes, typically more than ten, are attached to a dextran polymer. MHC-dextramers are described in the patent application WO 02/072631 A2.

• MHC streptamers: 8-12 MHC-peptide complexes attached to Streptactin. MHC streptamers are described in Knabel M et al. Reversibel MHC multimer staining for functional isolation of T-cell populations and effective adoptive transfer. Nature medicine 6. 631 -637 (2002).

Use of MHC multimers in flow cytometry and related techniques

The concentration of antigen-specific T-cells in samples from e.g. peripheral blood can be very low. Flow cytometry and related methods offer the ability to analyze a large number of cells and simultaneously identify the few of interest. MHC multimers have turned out to be very valuable reagents for detection and characterization of antigen- specific T-cells in flow cytometer experiments. The relative amount of antigen-specific T cells in a sample can be determined and also the affinity of the binding of MHC multimer to the T-cell receptor can be determined. The basic function of a flow cytometer is its ability to analyse and identify fluorochrome labelled entities in a liquid sample, by means of its excitation, using a light source such as a laser beam and the light emission from the bound fluorochrome. MHC multimers is used as detections molecule for identification of antigen-specific T- cells in flow cytometry, by labelling the MHC multimer with a specific fluorochrome, which is detectable, by the flow cytometer used. In order to facilitate the identification of a small amount of cells, the cells can be sub- categorized using antibodies or other fluorochrome labelled detections molecules directed against surface markers other than the TCR on the specific T-cells population. Antibodies or other fluorochrome labelled detections molecules can also be used to identify cells known not to be antigen-specific T-cells. Both kinds of detections molecules are in the following referred to as gating reagents. Gating reagents, helps identify the "true" antigen-specific T cells bound by MHC multimers by identifying specific subpopulations in a sample, e.g. T cells and by excluding cells that for some reason bind MHC mulimers without being antigen-specific T-cells. Other cytometry methods, e.g. fluorescence microscopy and IHC can like flow cytometry be employed in identification of antigen-specific T cells in a cell sample using MHC multimers.

Application of MHC multimers in immune monitoring, diagnostics, prognostics, therapy and vaccines

T cells are pivotal for mounting an adaptive immune response. It is therefore of importance to be able to measure the number of specific T cells when performing a monitoring of a given immune response, for example in connection with vaccine development, infectious diseases e.g. tuberculosis, toxicity studies etc.

Accordingly, the present invention further provides powerful tools in the fields of vaccines, therapy and diagnosis. One objective is to isolate antigen-specific T-cells and culture these in the presence of co-stimulatory molecules. Ex vivo priming and expansion of T-cell populations allows the T-cells to be used in immunotherapy of various types of infectious diseases. A second objective of the present invention is to identify and label specific subsets of cells with relevance for the development or treatment of diseases.

Of special interest of the present invention is transplantation related disease. MHC multimers of the present invention can be used in immune monitoring following transplantation but may also be used for immune monitoring in other immuno suppressive conditions e.g. in HIV infected patients or patients receiving chemotherapy SUMMARY OF INVENTION.

Measurement of antigen-specific T cells during an immune response are important parameters in vaccine development, autologous cancer therapy, transplantation, infectious diseases, inflammation, autoimmunity, toxicity studies etc. MHC multimers are crucial reagents in monitoring of antigen-specific T cells. The present invention describes novel methods to generate MHC multimers and methods to improve existing and new MHC multimers. The invention also describes improved methods for the use of MHC multimers in analysis of T cells in samples including diagnostic and prognostic methods. Furthermore the use of MHC multimers in therapy are described, e.g. anti- tumour and anti-virus therapy, including isolation of antigen-specific T cells capable of inactivation or elimination of undesirable target cells or isolation of specific T cells capable of regulation of other immune cells. The present invention also relates to MHC multimers comprising one or more CMV derived peptides. In one preferred embodyment such MHC multimers are used in monitoring immune status following e.g. transplantation, during immunosuppresive treatment, during anticancer treatment, in HIV infected individuals or other conditions where the immune system is supressed.

In one preferred embodiment the present invention relates to a MHC multimer such as a MHC dextramer such as a chemically biotinylated MHC dextramer. These MHC multimers prefereably comprise one or more peptides derived from one or more CMV antigens such as one or more peptides derived from pp28, pp50, pp65, pp150, pp71 , gH, gB, IE-1 , IE-2, US2, US3, US6, US1 1 , and UL18.

In one preferred embodiment the measurement of antigen-specific T cells according to the present invention is based on measurement of the number of T cells and not on measurement of activation of T cells.

In one preferred embodiment the measurement of antigen-specific T cells according to the present invention is based on a binding assay and not on a functional assay.

In another preferred embodiment the method for measurement of T cells is not based on and/or does not involve priming of Antigen presenting cells. The method for measurement of T cells can in one preferred embodiment be performed at experimental condition that will only allow limited or completely prevent priming of Antigen presenting cells. These experimental conditions can involve one or more factors that do not allow priming of Antigen presenting cells such as an incubation time that is too short to allow priming or a pH or salt concentration that will prevent or inhibit priming of Antigen presenting cells.

In another preferred embodiment the present invention relates to a CMV vaccine. In a CMV vaccine the peptides bound in the peptide binding cleft of MHC are derived from CMV proteins.

DEFINITIONS As used everywhere herein, the term "a", "an" or "the" is meant to be one or more, i. e. at least one.

Adjuvant: adjuvants are drugs that have few or no pharmacological effects by themselves, but can increase the efficacy or potency of other drugs when given at the same time. In another embodiment, an adjuvant is an agent which, while not having any specific antigenic effect in itself, can stimulate the immune system, increasing the response to a vaccine.

Agonist: agonist as used herein is a substance that binds to a specific receptor and triggers a response in the cell. It mimics the action of an endogenous ligand that binds to the same receptor.

Antagonist: antagonist as used herein is a substance that binds to a specific receptor and blocks the response in the cell. It blocks the action of an endogenous ligand that binds to the same receptor.

Antibodies: As used herein, the term "antibody" means an isolated or recombinant binding agent that comprises the necessary variable region sequences to specifically bind an antigenic epitope. Therefore, an antibody is any form of antibody or fragment thereof that exhibits the desired biological activity, e.g., binding the specific target antigen. Antibodies can derive from multiple species. For example, antibodies include rodent (such as mouse and rat), rabbit, sheep, camel, and human antibodies. Antibodies can also include chimeric antibodies, which join variable regions from one species to constant regions from another species. Likewise, antibodies can be humanized, that is constructed by recombinant DNA technology to produce immunoglobulins which have human framework regions from one species combined with complementarity determining regions (CDR's) from a another species' immunoglobulin. The antibody can be monoclonal or polyclonal. Antibodies can be divided into isotypes (IgA, IgG, IgM, IgD, IgE, IgGI , lgG2, lgG3, lgG4, IgAI , lgA2, IgMI , lgM2)

Antibodies: In another embodiment the term "antibody" refers to an intact antibody, or a fragment of an antibody that competes with the intact antibody for antigen binding. In certain embodiments, antibody fragments are produced by recombinant DNA techniques. In certain embodiments, antibody fragments are produced by enzymatic or chemical cleavage of intact antibodies. Exemplary antibody fragments include, but are not limited to, Fab, Fab', F(ab')2, Fv, and scFv. Exemplary antibody fragments also include, but are not limited to, domain antibodies, nanobodies, minibodies ((scFv- C.sub.H3).sub.2), maxibodies ((scFv-C.sub.H2-C. sub. H3).sub.2), diabodies (noncovalent dimer of scFv).

Antigen presenting cell: An antigen-presenting cell (APC) as used herein is a cell that displays foreign antigen complexed with MHC on its surface.

Antigenic peptide: Used interchangeably with binding peptide. Any peptide molecule that is bound or able to bind into the binding groove of either MHC class 1 or MHC class 2.

Aptamer: the term aptamer as used herein is defined as oligonucleic acid or peptide molecules that bind a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist. Aptamers can be divided into DNA aptamers, RNA aptamers and peptide aptamers.

Avidin: Avidin as used herein is a glycoprotein found in the egg white and tissues of birds, reptiles and amphibians. It contains four identical subunits having a combined mass of 67,000-68,000 daltons. Each subunit consists of 128 amino acids and binds one molecule of biotin.

Biologically active molecule: A biologically active molecule is a molecule having itself a biological activity/effect or is able to induce a biological activity/effect when administered to a biological system. Biologically active molecules include adjuvants, immune targets (e.g. antigens), enzymes, regulators of receptor activity, receptor ligands, immune potentiators, drugs, toxins, cytotoxic molecules, co-receptors, proteins and peptides in general, sugar moieties, lipid groups, nucleic acids including siRNA, nanoparticles, and small molecules.

Bioluminescent: Bioluminescence, as used herein, is the production and emission of light by a living organism as the result of a chemical reaction during which chemical energy is converted to light energy.

Biotin: Biotin, as used herein, is also known as vitamin H or B₇. Niotin has the chemical formula Ci₀H₁₆N₂O₃S.

Bispecific antibodies: The term bispecific antibodies as used herein is defined as monoclonal, preferably but not limited to human or humanized, antibodies that have binding specificities for at least two different antigens. The antibody can also be trispecific or multispecific.

Carrier: A carrier as used herin can be any type of molecule that is directly or indirectly associated with the MHC peptide complex. In this invention, a carrier will typically refer to a functionalized polymer (e.g. dextran) that is capable of reacting with MHC-peptide complexes, thus covalently attaching the MHC-peptide complex to the carrier, or that is capable of reacting with scaffold molecules (e.g. streptavidin), thus covalently attaching streptavidin to the carrier; the streptavidin then may bind MHC-peptide complexes. Carrier and scaffold are used interchangeably herein where scaffold typically refers to smaller molecules of a multimerization domain and carrier typically refers to larger molecule and/or cell like structures.

Chelating chemical compound: Chelating chemical compound, as used herein, is the process of reversible bindingof a ligand to a metal ion, forming a metal complex.

Chemiluminescent: Chemiluminescence, as used herein, is the emission of light (luminescence) without emission of heat as the result of a chemical reaction. Chromophore: A chromophore, as used herein, is the part of a visibly coloured molecule responsible for light absorption over a range of wavelengths thus giving rise to the colour. By extension the term can be applied to uv or ir absorbing parts of molecules.

CMV: Cytomegalovirus

Coiled-coil polypeptide: Used interchangeably with coiled-coil peptide and coiled-coil structure. The term coiled-coil polypeptide as used herein is a structural motif in proteins, in which 2-7 alpha-helices are coiled together like the strands of a rope

Covalent binding: The term covalent binding is used herein to describe a form of chemical bonding that is characterized by the sharing of pairs of electrons between atoms. Attraction-to-repulsion stability that forms between atoms when they share electrons is known as covalent bonding.

Crosslinking is the process of chemically joining two or more molecules by a covalent bond. Crosslinking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules.

Diagnosis: The act or process of identifying or determining the nature and cause of a disease or injury through evaluation

Diabodies: The term "diabodies" refers to small antibody fragments with two antigen- binding sites, which fragments comprise a heavy-chain variable domain (VH) connected to a light-chain variable domain (VL) in the same polypeptide chain (VH-VL). By using a linker that is too short to allow pairing between the two domains on the same chain, the domains are forced to pair with the complementary domains of another chain and create two antigen-binding sites.

Dendritic cell: The term dendritic cell as used herein is a type of immune cells. Their main function is to process antigen material and present it on the surface to other cells of the immune system, thus functioning as antigen-presenting cells. Detection: In this invention detection means any method capable of measuringen one molecule bound to anoher molecule. The molecules are typically proteins but can be any type of molecule

Dextran: the term dextran as used herein is is a complex, branched polysaccharide made of many glucose molecules joined into chains of varying lengths. The straight chain consists of α1 ->6 glycosidic linkages between glucose molecules, while branches begin from α1 ->3 linkages (and in some cases, α1 ->2 and α1 ->4 linkages as well).

Direct detection of T cells: Direct detection of T cells is used herein interchangeably with direct detection of TCR and direct detection of T cell receptor. As used herein direct detection of T cells is detection directly of the binding interaction between a specific T cell receptor and a MHC multimer.

DNA: The term DNA (Deoxyribonucleic acid) duplex as used herein is a polymer of simple units called nucleotides, with a backbone made of sugars and phosphate atoms joined by ester bonds. Attached to each sugar is one of four types of molecules called bases.

DNA duplex: In living organisms, DNA does not usually exist as a single molecule, but instead as a tightly-associated pair of molecules. These two long strands entwine like vines, in the shape of a double helix.

Electrophilic: electrophile, as used herein, is a reagent attracted to electrons that participates in a chemical reaction by accepting an electron pair in order to bond to a nucleophile.

Enzyme label: enzyme labelling, as used herein, involves a detection method comprising a reaction catalysed by an enzyme.

Epitope-focused antibody: Antibodies also include epitope-focused antibodies, which have at least one minimal essential binding specificity determinant from a heavy chain or light chain CDR3 from a reference antibody, methods for making such epitope- focused antibodies are described in U.S. patent application Ser. No. 1 1/040,159, which is incorporated herein by reference in its entirety. Flow cytomerty: The analysis of single cells using a flow cytometer.

Flow cytometer: Instrument that measures cell size, granularity and flourescence due to bound fluorescent marker molecules as single cells pass in a stream past photodectors. A flow cytomter carry out the measurements and/or sorting of individual cells.

Fluorescent: the term fluorescent as used herein is to have the ability to emit light of a certain wavelength when activated by light of another wavelength.

Fluorochromes: fluorochrome, as used herein, is any fluorescent compound used as a dye to mark e.g. protein with a fluorescent label.

Fluorophore: A fluorophore, as used herein, is a component of a molecule which causes a molecule to be fluorescent.

Folding: In this invention folding means in vitro or in vivo folding of proteins in a tertiery structure.

Fusion antibody: As used herein, the term "fusion antibody" refers to a molecule in which an antibody is fused to a non-antibody polypeptide at the N- or C-terminus of the antibody polypeptide.

Glycosylated: Glycosylation, as used herein, is the process or result of addition of saccharides to proteins and lipids.

Hapten: A residue on a molecule for which there is a specific molecule that can bind, e.g. an antibody.

Heteroconjugate antibodies are composed of two covalently joined antibodies. Such antibodies have, for example, been proposed to target immune system cells to unwanted cells. IgG: IgG as used herein is a monomeric immunoglobulin, built of two heavy chains and two light chains. Each molecule has two antigen binding sites.

Isolated antibody: The term "isolated" antibody as used herein is an antibody which has been identified and separated and/or recovered from a component of its natural environment.

Immunoconjugates: The invention also pertains to immunoconjugates comprising an antibody or a MHC-peptide complex conjugated to a cytotoxic agent such as a chemotherapeutic agent, toxin (e.g., an enzymatically active toxin of bacterial, fungal, plant, or animal origin, or fragments thereof), or a radioactive isotope (i.e., a radioconjugate). Enzymatically active toxins and fragments thereof that can be used include diphtheria A chain, nonbinding active fragments of diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa), ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin, Aleurites fordii proteins, dianthin proteins, Phytolaca americana proteins (PAPI, PAPII, and PAP-S), momordica charantia inhibitor, curcin, crotin, sapaonaria officinalis inhibitor, gelonin, mitogellin, restrictocin, phenomycin, enomycin, and the tricothecenes. A variety of radionuclides are available for the production of radioconjugated antibodies or MHC-peptide complexes. Conjugates of the antibody or MHC-peptide complex and cytotoxic agent are made using a variety of bifunctional protein-coupling agents such as N-succinimidyl-3-(2-pyridyldithiol) propionate (SPDP), iminothiolane (IT), bifunctional derivatives of imidoesters (such as dimethyl adipimidate HCL), active esters (such as disuccinimidyl suberate), aldehydes (such as glutaraldehyde), bis-azido compounds (such as bis(p-azidobenzoyl)hexanediamine), bis-diazonium derivatives (such as bis-(p-diazoniumbenzoyl)-ethylenediamine), diisocyanates (such as tolyene 2,6-diisocyanate), and bis-active fluorine compounds (such as 1 ,5-difluoro-2,4-dinitrobenzene).

Immune monitoring: Immune monitoring of the present invention refers to testing of immune status in the diagnosis and therapy of diseases like but not limited to cancer, immunoproliferative and immunodeficiency disorders, autoimmune abnormalities, and infectious diseases. It also refers to testing of immune status before, during and after vaccination and transplantation procedures.

Immune monitoring process: a series of one or more immune monitoring analysis lmmuno profiling: lmmuno profiling as used herein defines the profiling of an individual's antigen-specific T-cell repertoire

Indirect detection of T cells: Indirect detection of T cells is used interchangeably herein with Indirect detection of TCR and indirect detection of T cell receptor. As used herein indirect detection of T cells is detection of the binding interaction between a specific T cell receptor and a MHC multimer by measurement of the effect of the binding interaction.

lonophore: ionophore, as used herein, is a lipid-soluble molecule usually synthesized by microorganisms capable of transporting ions.

Label: Label herein is used interchangeable with labeling molecule. Label as described herein is an identifiable substance that is detectable in an assay and that can be attached to a molecule creating a labeled molecule. The behavior of the labeled molecule can then be studied.

Labelling: Labelling herein means attachment of a label to a molecule.

Lanthanide: lanthanide, as used herein, series comprises the 15 elements with atomic numbers 57 through 71 , from lanthanum to lutetium.

Linker molecule: Linker molecule and linker is used interchangeable herein. A linker molecule is a molecule that covalently or non-covalently connects two or more molecules, thereby creating a larger complex consisting of all molecules including the linker molecule.

Liposomes: The term liposomes as used herein is defined as a spherical vesicle with a membrane composed of a phospholipid and cholesterol bilayer. Liposomes, usually but not by definition, contain a core of aqueous solution; lipid spheres that contain no aqueous material are called micelles.

Immunoliposomes: The antibodies or MHC-peptide complexes disclosed herein can also be formulated as immunoliposomes. Liposomes comprising the antibody or MHC- peptide complexes are prepared by methods known in the art, such as described in Epstein et al., Proc. Natl. Acad. Sci. USA 82: 3688 (1985); Hwang et al., Proc. Natl. Acad. Sci. USA 77: 4030 (1980); and U.S. Pat. Nos. 4,485,045 and 4,544,545. Particularly useful liposomes can be generated by the reverse-phase evaporation method with a lipid composition comprising phosphatidylcholine, cholesterol, and PEG- derivatized phosphatidylethanolamine (PEG-PE).

Marker: Marker is used interchangeably with marker molecule herein. A marker is molecule that specifically associates covalently or non-covalently with a molecule belonging to or associated with an entity.

MHC: Denotes the major histocompatibility complex.

MHC I is used interchangeably herein with MHC class I and denotes the major histocompatibility complex class I.

MHC Il is used interchangeably herein with MHC class Il and denotes the major histocompatibility complex class I.

MHC molecule: a MHC molecule as used everywhere herein is defined as any MHC class I molecule or MHC class Il molecule as defined herein.

A "MHC Class I molecule" as used everywhere herein is used interchangeably with MHC I molecule and is defined as a molecule which comprises 1 -3 subunits, including a MHC I heavy chain, a MHC I heavy chain combined with a MHC I beta2microglobulin chain, a MHC I heavy chain combined with MHC I beta2microglobulin chain through a flexible linker, a MHC I heavy chain combined with an antigenic peptide, a MHC I heavy chain combined with an antigenic peptide through a linker, a MHC I heavy chain/ MHC I beta2microglobulin dimer combined with an antigenic peptide, and a MHC I heavy chain/ MHC I beta2microglobulin dimer combined with an antigenic peptide through a flexible linker to the heavy chain or beta2microglobulin. The MHC I molecule chains can be changed by substitution of single or by cohorts of native amino acids, or by inserts, or deletions to enhance or impair the functions attributed to said molecule. MHC complex: MHC complex is herein used interchangeably with MHC-peptide complex, and defines any MHC I and/or MHC Il molecule combined with antigenic peptide unless it is specified that the MHC complex is empty, i.e. is not complexed with antigenic peptide

MHC Class I like molecules (including non-classical MHC Class I molecules) include CD1d, HLA E, HLA G, HLA F, HLA H, MIC A, MIC B, ULBP-1 , ULBP-2, and ULBP-3.

A "MHC Class Il molecule" as used everywhere herein is used interchangeably with MHC Il molecule and is defined as a molecule which comprises 2-3 subunits including a MHC Il alpha-chain and a MHC Il beta-chain (i.e. a MHC Il alpha/beta-dimer), an MHC Il alpha/beta dimer with an antigenic peptide, and an MHC Il alpha/beta dimer combined with an antigenic peptide through a flexible linker to the MHC Il alpha or MHC Il beta chain, a MHC Il alpha/beta dimer combined through an interaction by affinity tags e.g. jun-fos, a MHC Il alpha/beta dimer combined through an interaction by affinity tags e.g. jun-fos and further combined with an antigenic peptide through a flexible linker to the MHC Il alpha or MHC Il beta chain. The MHC Il molecule chains can be changed by substitution of single or by cohorts of native amino acids, or by inserts, or deletions to enhance or impair the functions attributed to said molecule. Under circumstances where the MHC Il alpha-chain and MHC Il beta-chain have been fused, to form one subunit, the "MHC Class Il molecule" can comprise only 1 subunit or 2 subunits if antigenic peptide also. Included.

MHC Class Il like molecules (including non-classical MHC Class Il molecules) include HLA DM, HLA DO, I-A beta2, and I-E beta2.

A "peptide free MHC Class I molecule" is used interchangeably herein with "peptide free MHC I molecule" and as used everywhere herein is meant to be a MHC Class I molecule as defined above with no peptide.

A "peptide free MHC Class Il molecule" is used interchangeably herein with "peptide free MHC Il molecule" and as used everywhere herein is meant to be a MHC Class Il molecule as defined above with no peptide. o

Such peptide free MHC Class I and Il molecules are also called "empty" MHC Class I and Il molecules.

The MHC molecule may suitably be a vertebrate MHC molecule such as a human, a mouse, a rat, a porcine, a bovine or an avian MHC molecule. Such MHC complexes from different species have different names. E.g. in humans, MHC complexes are denoted HLA. The person skilled in the art will readily know the name of the MHC complexes from various species.

In general, the term "MHC molecule" is intended to include all alleles. By way of example, in humans e.g. HLA A, HLA B, HLA C, HLA D, HLA E, HLA F, HLA G, HLA H, HLA DR, HLA DQ and HLA DP alleles are of interestshall be included, and in the mouse system, H-2 alleles are of interestshall be included. Likewise, in the rat system RT1 -alleles, in the porcine system SLA-alleles, in the bovine system BoLA, in the avian system e.g. chicken-B alleles, are of interestshall be included.

"MHC complexes" and "MHC constructs" are used interchangeably herein.

By the terms "MHC complexes" and "MHC multimers" as used herein are meant such complexes and multimers thereof, which are capable of performing at least one of the functions attributed to said complex or multimer. The terms include both classical and non-classical MHC complexes. The meaning of "classical" and "non-classical" in connection with MHC complexes is well known to the person skilled in the art. Non- classical MHC complexes are subgroups of MHC-like complexes. The term "MHC complex" includes MHC Class I molecules, MHC Class Il molecules, as well as MHC- like molecules (both Class I and Class II), including the subgroup non-classical MHC Class I and Class Il molecules.

MHC multimer: The terms MHC multimer, MHC-multimer, MHCmer and MHC'mer herein are used interchangeably, to denote a complex comprising more than one MHC- peptide complexes, held together by covalent or non-covalent bonds.

Monoclonal antibodies: Monoclonal antibodies, as used herein, are antibodies that are identical because they were produced by one type of immune cell and are all clones of a single parent cell. Monovalent antibodies: The antibodies in the present invention can be monovalent antibodies. Methods for preparing monovalent antibodies are well known in the art. For example, one method involves recombinant expression of immunoglobulin light chain and modified heavy chain. The heavy chain is truncated generally at any point in the Fc region so as to prevent heavy chain crosslinking. Alternatively, the relevant cysteine residues are substituted with another amino acid residue or are deleted so as to prevent crosslinking. In vitro methods are also suitable for preparing monovalent antibodies. Digestion of antibodies to produce fragments thereof, particularly, Fab fragments, can be accomplished using routine techniques known in the art.

Multimerization domain: A multimerization domain is a molecule, a complex of molecules, or a solid support, to which one or more MHC or MHC-peptide complexes can be attached. A multimerization domain consist of one or more carriers and/or one or more scaffolds and may also contain one or more linkers connecting carrier to scaffold, carrier to carrier, scaffold to scaffold. The multimerization domain may also contain one or more linkers that can be used for attachment of MHC complexes and/or other molecules to the multimerization domain. Multimerization domains thus include IgG, streptavidin, streptactin, micelles, cells, polymers, beads and other types of solid support, and small organic molecules carrying reactive groups or carrying chemical motifs that can bind MHC complexes and other molecules.

Nanobodies: Nanobodies as used herein is a type of antibodies derived from camels, and are much smaller than traditional antibodies.

Neutralizing antibodies: neutralizing antibodies as used herein is an antibody which, on mixture with the homologous infectious agent, reduces the infectious titer.

NMR: NMR (Nuclear magnetic resonance), as used herein, is a physical phenomenon based upon the quantum mechanical magnetic properties of an atom's nucleus. NMR refers to a family of scientific methods that exploit nuclear magnetic resonance to study molecules. Non-covalent: The term noncovalent bond as used herein is a type of chemical bond, that does not involve the sharing of pairs of electrons, but rather involves more dispersed variations of electromagnetic interactions.

Nucleic acid duplex: A nucleic acid is a complex, high-molecular-weight biochemical macromolecule composed of nucleotide chains that convey genetic information. The most common nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).

Nucleophilic: a nucleophile, as used herein, is a reagent that forms a chemical bond to its reaction partner (the electrophile) by donating both bonding electrons.

"One or more" as used everywhere herein is intended to include one and a plurality.

A "peptide free MHC Class I molecule" as used everywhere herein is meant to be a MHC Class I molecule as defined above with no peptide.

A "peptide free MHC Class Il molecule" as used everywhere herein is meant to be a MHC Class Il molecule as defined above with no peptide.

Pegylated: pegylated, as used herein, is conjugation of Polyethylene glycol (PEG) to proteins.

Pentamer, MHC pentamer and pentamer MHC multimer is used interchangeable herein and refers to a MHC multimer comprising 5 MHC molecules and optionally one or more labelling compunds.

Peptide or protein: Any molecule composed of at least two amino acids. Peptide normally refers to smaller molecules of up to around 30 amino acids and protein to larger molecules containing more amino acids. Phosphorylated; phosphorylated, as used herein, is is the addition of a phosphate (PO₄) group to a protein molecule or a small molecule. "A plurality" as used everywhere herein should be interpreted as two or more. PNA: PNA (Peptide nucleic acid) as used herein is a chemical similar to DNA or RNA. PNA is not known to occur naturally in existing life on Earth but is artificially synthesized and used in some biological research and medical treatments. DNA and RNA have a deoxyribose and ribose sugar backbone, respectively, whereas PNA's backbone is composed of repeating N-(2-aminoethyl)-glycine units linked by peptide bonds. The various purine and pyrimidine bases are linked to the backbone by methylene carbonyl bonds. PNAs are depicted like peptides, with the N-terminus at the first (left) position and the C-terminus at the right.

"A plurality" as used everywhere herein should be interpreted as two or more. This applies i.a. to the MHC peptide complex and the binding entity. When a plurality of MHC peptide complexes is attached to the multimerization domain, such as a scaffold or a carrier molecule, the number of MHC peptide complexes need only be limited by the capacity of the multimerization domain.

Polyclonal antibodies: a polyclonal antibody as used herein is an antibody that is derived from different B-cell lines. They are a mixture of immunoglobulin molecules secreted against a specific antigen, each recognising a different epitope.

Polymer: the tern polymer as used herein is defined as a compound composed of repeating structural units, or monomers, connected by covalent chemical bonds.

Polypeptide: Peptides are the family of short molecules formed from the linking, in a defined order, of various α-amino acids. The link between one amino acid residue and the next is an amide bond and is sometimes referred to as a peptide bond. Longer peptides are reffered to as proteins or polypeptide.

Polysaccharide: The term polysaccharide as used herein is defined as polymers made up of many monosaccharides joined together by glycosidic linkages. Radicals: radicals, as used herein, are atomic or molecular species with unpaired electrons on an otherwise open shell configuration. These unpaired electrons are usually highly reactive, so radicals are likely to take part in chemical reactions.

Radioactivity: Radioactive decay is the process in which an unstable atomic nucleus loses energy by emitting radiation in the form of particles or electromagnetic waves. RNA: RNA (Ribonucleic acid) as used herein is a nucleic acid polymer consisting of nucleotide monomers that plays several important roles in the processes that translate genetic information from deoxyribonucleic acid (DNA) into protein products

Scaffold: A scaffold is typically an organic molecule carrying reactive groups, capable of reacting with reactive groups on a MHC-peptide complex. Particularly small organic molecules of cyclic structure (e.g. functionalized cycloalkanes or functionalized aromatic ring structures) are termed scaffolds. Scaffold and carrier are used interchangeably herein where scaffold typically refers to smaller molecules of a multimerization domain and carrier typically refers to larger molecule and/or cell like structures.

Staining: In this invention staining means specific or unspecific labelling of cells by binding labeled molecules to defined proteins or other structures on the surface of cells or inside cells. The cells are either in suspension or part of a tissue. The labeled molecules can be MHC multimers, antibodies or similar molecules capable of binding specific structures on the surface of cells.

Streptavidin: Streptavidin as used herein is a tetrameric protein purified from the bacterium Streptomyces avidinii. Streptavidin is widely use in molecular biology through its extraordinarily strong affinity for biotin.

Sugar: Sugars as used herein include monosaccharides, disaccharides, trisaccharides and the oligosaccharides - comprising 1 , 2, 3, and 4 or more monosaccharide units respectively.

Therapy: Treatment of illness or disability Vaccine: A vaccine is an antigenic preparation used to establish immunity to a disease or illness and thereby protects or cure the body from a specific disease or illness. Vaccines are either prophylactic and prevent disease or therapeutic and treat disease. Vaccines may contain more than one type of antigen and is then called a combined vaccine.

Vaccination: The introduction of vaccine into the body of human or animals for the purpose of inducing immunity.

B. L. is an abereviation for Bind level

Aff. Is an abbreviation for affinity

DETAILED DESCRIPTION OF INVENTION. The present invention in one aspect refers to a MHC monomer comprising a-b-P, or a MHC multimer comprising (a-b-P)_n, wherein n > 1 ,

wherein a and b together form a functional MHC protein capable of binding the peptide P,

wherein (a-b-P) is the MHC-peptide complex formed when the peptide P binds to the functional MHC protein, and

wherein each MHC peptide complex of a MHC multimer is associated with one or more multimerization domains.

The peptide is in one embodiment a CMV peptide such as e.g. a peptide derived from the CMV internal matrix protein pp65.

MHC monomers and MHC multimers comprising one or more MHC peptide complexes of class 1 or class 2 MHC are covered by the present invention. Accordingly, the peptide P can have a length of e.g. 8, 9 ,10, 1 1 , 12, 13, 14, 15, 16, 16-20, or 20-30 amino acid residues.

Examples of the peptide P is provided herein below. In one embodiment, the peptide P can be selected from the group consisting of sequences disclosed in the electronically enclosed "Sequence Listing" and annotated consecutively (using integers) starting with SEQ ID NO 1 and ending with SEQ ID NO 9697.

In another aspect the present invention is directed to a composition comprising a plurality of MHC monomers and/or MHC multimers according to the present invention, wherein the MHC multimers are identical or different, and a carrier.

In yet another aspect there is provided a kit comprising a MHC monomer or a MHC multimer according to the present invention, or a composition according to the present invention, and at least one additional component, such as a positive control and/or instructions for use.

In a still further aspect there is provided a method for immune monitoring one or more diseases comprising monitoring of antigen-specific T cells, said method comprising the steps of

i) providing the MHC monomer or MHC multimer or individual components thereof according to the present invention, or the individual components thereof, ii) providing a population of antigen-specific T cells or individual antigen-specific T cells, and iii) measuring the number, activity or state and/or presence of antigen-specific of T cells specific for the peptide P of thesaid MHC monomer or MHC multimer, thereby immune monitoring said one or more diseases.

In yet another aspect there is provided a method for diagnosing one or more diseases comprising immune monitoring of antigen-specific T cells, said method comprising the following steps: of

i) providing the MHC monomer or MHC multimer or individual components thereof according to the present invention, or individual components thereof, ii) providing a population of antigen-specific T cells or individual antigen-specific T cells, and iii) measuring the number, activity or state and/or presence of T cells specific for said

MHC monomer or the peptide P of the MHC multimer, thereby diagnosing said one or more diseases. There is also provided a method for isolation of one or more antigen-specific T cells, said method comprising the steps of

i) providing the MHC monomer or MHC multimer or individual components thereof according to the present invention, or individual components thereof, and ii) providing a population of antigen-specific T cells or individual antigen-specific T cells, and iii) thereby isolating said T cells specific for the peptide P of the said MHC monomer or MHC multimer.

The present invention makes it possible to pursue different immune monitoring methods using the MHC monomers and MHC multimers according to the present invention. The immune monitoring methods include e.g. flow cytometry, ELISPOT, LDA, Quantaferon and Quantaferon-like methods. Using the above-cited methods, the MHC monomers and/or the MHC multimers can be provided as a MHC peptide complex, or the peptide and the MHC monomer and/or multimer can be provided separately.

Accordingly, recognition of TCR's can be achieved by direct or indirect detection, e.g. by using one or more of the following methods:

ELISPOT technique using indirect detection, e.g. by adding the antigenic peptide optionally associated with a MHC monomer or MHC multimer, followed by measurement of INF-gamma secretion from a population of cells or from individual cells.

Another technique involves a Quantaferon-like detection assays, e.g. by using indirect detection, e.g. by adding the antigenic peptide optionally associated with a MHC monomer or MHC multimer, followed by measurement of INF-gamma secretion from a population of cells or from individual cells.

Flow cytometry offers another alternative for performing detection assays, e.g. by using direct detection (e.g. of MHC tetramers), e.g. by adding the antigenic peptide optionally associated with a MHC monomer or MHC multimer, followed by detection of a fluorescein label, thereby measuring the number of TCRs on specific T-cells.

Flow cytometry can also be used for indirect detection, e.g. by adding the antigenic peptide optionally associated with a MHC monomer or MHC multimer, followed by addition of a "cell-permeabilizing factor", and subsequent measurement of an intracellular component (e.g. INF-gamma mRNA), from individual cells or populations of cells.

By using the above-mentioned and other techniques, one can diagnose and/or monitor e.g. infectious diseases caused e.g. by mycobacetrium, Gram positive bacteria, Gram negative bacteria, Spirochetes, intracellular bacterium, extracelular bacterium, Borrelia, TB, CMV, HPV, Hepatitis, BK, fungal organisms and microorganisms. The diagnosis and/or monitoring of a particular disease can greatly aid in directing an optimal treatment of said disease in an individual.

Cancer monitoring methods also fall within the scope of the present invention.

In still further aspects of the present invention there is provided a method for performing a vaccination of an individual in need thereof, said method comprising the steps of

providing a MHC monomer or a MHC multimer according to the present invention, or the individual components thereof, and

administering said MHC monomer or MHC multimer to said individual and obtaining a protective immune response, thereby performing a vaccination of the said individual.

In yet another embodiment there is provided a method for performing therapeutic treatment of an individual comprising the steps of

Providing the MHC multimer according to the present invention, or individual components thereof, and Isolating or obtaining T-cells from a source, such as an individual or an ex-vivo library or cell bank, wherein said isolated or obtained T-cells are specific for said provided MHC multimer,

Optionally manipulating said T-cells, and

Introducing said isolated or obtained T-cells into an individual to be subjected to a therapeutic treatment, wherein the individual can be the same individual or a different individual from the source individual.

There is also provided a method comprising one or more steps for minimizing undesired binding of the MHC multimer according to the present invention. This method is disclosed herein below in more detail.

In further aspects the present invention provides:

A method for performing a control experiment comprising the step of counting of particles comprising the MHC multimer according to the present invention.

A method for performing a control experiment comprising the step of sorting of particles comprising the MHC multimer according to the present invention.

A method for performing a control experiment comprising the step of performing flow cytometry analysis of particles comprising the MHC multimer according to the present invention.

A method for performing a control experiment comprising the step of performing a immunohistochemistry analysis comprising the MHC multimer according to the present invention.

A method for performing a control experiment comprising the step of performing a immunocytochemistry analysis comprising the MHC multimer according to the present invention. A method for performing a control experiment comprising the step of performing an ELISA analysis comprising the MHC multimer according to the present invention.

In a still further aspect of the present invention there is provided a method for generating MHC multimers according to the present invention, said method comprising the steps of

i) providing one or more peptides P; and/or ii) providing one or more functional MHC proteins, iii) optionally providing one or more multimerization domains, and iv) contacting the one or more peptides P and the one or more functional MHC proteins and the one or more multimerization domains simultaneously or sequentialy in any order, thereby obtaining MHC multimers according to the present invention.

The method can also be performed by initially providing one or more antigenic peptide(s) P and one or more functional MHC proteins to generate a MHC-peptide complex (a-b-P); subsequently providing one or more multimerisation domain(s); and reacting the one or more MHC-peptide complexes and the one or more multimerization domain(s) to generate a MHC multimer according to the present invention.

In one aspect, the present invention is directed to novel MHC complexes optionally comprising a multimerization domain preferably comprising a carrier molecule and/or a scaffold.

There is also provided a MHC multimer comprising 2 or more MHC-peptide complexes and a multimerization domain to which the 2 or more MHC-peptide complexes are associated. The MHC multimer can generally be formed by association of the 2 or more MHC-peptide complexes with the multimerization domain to which the 2 or more MHC-peptide complexes are capable of associating.

The multimerization domain can be a scaffold associated with one or more MHC- peptide complexes, or a carrier associated with one or more, preferably more than one, MHC-peptide complex(es), or a carrier associated with a plurality of scaffolds each associated with one or more MHC-peptide complexes, such as 2 MHC-peptide complexes, 3 MHC-peptide complexes, 4 MHC-peptide complexes, 5 MHC-peptide complexes or more than 5 MHC-peptide complexes. Accordingly, multimerization domain collectively refers to each and every of the above. It will be clear from the detailed description of the invention provided herein below when the multimerization domain refers to a scaffold or a carrier or a carrier comprising one or more scaffolds.

Generally, when a multimerization domain comprising a carrier and/or a scaffold is present, the MHC complexes can be associated with this domain either directly or via one or more binding entities. The association can be covalent or non-covalent.

Accordingly, there is provided in one embodiment a MHC complex comprising one or more entities (a-b-P)_n, wherein a and b together form a functional MHC protein capable of binding a peptide P, and wherein (a-b-P) is the MHC-peptide complex formed when the peptide P binds to the functional MHC protein, said MHC complex optionally further comprising a multimerization domain comprising a carrier molecule and/or a scaffold. "MHC complex" refers to any MHC complex, including MHC monomers in the form of a single MHC-peptide complex and MHC multimers comprising a multimerization domain to which more than one MHC peptide complex is associated.

When the invention is directed to complexes comprising a MHC multimer, i.e. a plurality of MHC peptide complexes of the general composition (a-b-P)_n associated with a multimerization domain, n is by definition more than 1 , i.e. at least 2 or more. Accordingly, the term "MHC multimer" is used herein specifically to indicate that more than one MHC-peptide complex is associated with a multimerization domain, such as a scaffold or carrier or carrier comprising one or more scaffolds. Accordingly, a single MHC-peptide complex can be associated with a scaffold or a carrier or a carrier comprising a scaffold and a MHC-multimer comprising 2 or more MHC-peptide complexes can be formed by association of the individual MHC-peptide complexes with a scaffold or a carrier or a carrier comprising one or more scaffolds each associated with one or more MHC-peptide complexes.

When the MHC complex comprises a multimerization domain to which the n MHC- peptide complexes are associated, the association can be a covalent linkage so that each or at least some of the n MHC-peptide complexes is covalently linked to the multimerization domain, or the association can be a non-covalent association so that each or at least some of the n MHC-peptide complexes are non-covalently associated with the multimerization domain.

The MHC complexes of the invention may be provided in non-soluble or soluble form, depending on the intended application.

Effective methods to produce a variety of MHC complexes comprising highly polymorphic human HLA encoded proteins makes it possible to perform advanced analyses of complex immune responses, which may comprise a variety of peptide epitope specific T-cell clones.

One of the benefits of the MHC complexes of the present invention is that the MHC complexes overcome low intrinsic affinities of monomer ligands and counter receptors. The MHC complexes have a large variety of applications that include targeting of high affinity receptors (e.g. hormone peptide receptors for insulin) on target cells. Taken together poly-ligand binding to target cells has numerous practical, clinical and scientifically uses.

Thus, the present invention provides MHC complexes which present mono-valent or multi-valent binding sites for MHC recognising cells, such as MHC complexes optionally comprising a multimerization domain, such as a scaffold or a carrier molecule, which multimerization domain have attached thereto, directly or indirectly via one or more linkers, covalently or non-covalently, one or more MHC peptide complexes. "One or more" as used herein is intended to include one as well as a plurality, such as at least 2. This applies i.a. to the MHC peptide complexes and to the binding entities of the multimerization domain. The scaffold or carrier molecule may thus have attached thereto a MHC peptide complex or a plurality of such MHC peptide complexes, and/or a linker or a plurality of linkers.

PRODUCT

The product of the present invention is a MHC monomer or a MHC multimer as described above. As used in the description of this invention, the term "MHC multimers" will be used interchangeably with the terms MHC'mers and MHCmers, and will include any number, (larger than one) of MHC-peptide complexes, held together in a large complex by covalent or non-covalent interactions between a multimerization domain and one or more MHC-peptide complexes, and will also include the monomeric form of the MHC-peptide complex, i.e. a MHC-peptide complex that is not attached to a multimerization domain. The multimerization domain consists of one or more carriers and/or one or more scaffolds while the MHC-peptide complex consists of MHC molecule and antigenic peptide. MHC-peptide complexes may be attached to the multimerization domain through one or more linkers. A schematic representation of a MHC multimer is presented in figure 1.

Design and generation of antigenic peptides

Approaches and methods for the identification and design of appropriate peptides

MHC class 1 protein typically binds octa-, nona-, deca- or ondecamer (8-, 9-, 10,- 1 1 - mer) peptides in their peptide binding groove. The individual MHC class 1 alleles have individual preferences for the peptide length within the given range. MHC class 2 proteins typically bind peptides with a total length of 13-18 amino acids, comprising a 9'-mer core motif containing the important amino acid anchor residues. However the total length is not strictly defined, as opposed to most MHC class 1 molecules.

For some of the MHC alleles the optimal peptide length and the preferences for specific amino acid residues in the so called anchor positions are known.

To identify high-afinity binding peptides derived from a specific protein for a given MHC allele it is necessary to systematically work through the amino acid sequence of the protein to identify the putative high-affinity binding peptides. Although a given peptide is a binder it is not necessarily a functional T-cell epitope. Functionality needs to be confirmed by a functional analysis e.g. ELISPOT, CTL killing assay or flow cytometry assay.

The binding affinity of the peptide for the MHC molecules can for some MHC molecules be predicted in databases such as www.syfpeithi.de; http://www- bimas.cit.nih.gov/molbio/hla_bind/; www.cbs.dtu.dk/services/NetMHC/; www.cbs.dtu.dk/services/NetMHCII/

Design of binding peptides The first step in the design of binding peptides is obtaining the protein's amino acid seguence. When only the genomic DNA sequences are known, i.e. the reading frame and direction of transcription of the genes is unknown, the DNA sequence needs to be translated in all three reading frames in both directions leading to a total of six amino acid sequences for a given genome. From these amino acid sequences binding peptides can then be identified as described below. In organisms having intron/exon gene structure the present approach must be modified accordingly, to identify peptide sequence motifs that are derived by combination of amino acid sequences derived partly from two separate introns. cDNA sequences can be translated into the actual amino acid sequences to allow peptide identification. In cases where the protein sequence is known, these can directly be used to predict peptide epitopes.

Binding peptide sequences can be predicted from any protein sequence by either a total approach, generating binding peptide sequences for potentially any MHC allele, or by a directed approach, identifying a subset of binding peptides with certain preferred characteristics such as affinity for MHC protein, specificity for MHC protein, likelihood of being formed by proteolysis in the cell, and other important characteristics.

Design of MHC class 1 binding peptide sequence

Many parameters influence the design of the individual binding peptide, as well as the choice of the set of binding peptides to be used in a particular application. Important characteristics of the MHC-peptide complex are physical and chemical (e.g. proteolytic) stability. The relevance of these parameters must be considered for the production of the MHC-peptide complexes and the MHC multimers, as well as for their use in a given application. As an example, the stability of the MHC-peptide complex in assay buffer (e.g. PBS), in blood, or in the body can be very important for a particular application. In the interaction of the MHC-peptide complex with the TCR, a number of additional characteristics must be considered, including binding affinity and specificity for the TCR, degree of cross-talk, undesired binding or interaction with other TCRs. Finally, a number of parameters must be considered for the interaction of MHC-peptide complexes or MHC multimers with the sample or individual it is being applied to. These include immunogenicity, allergenicity, as well as side effects resulting from un-desired interaction with "wrong" T cells, including cross-talk with e.g. autoimmune diseases and un-desired interaction with other cells than antigen-specific T cells. For some applications, e.g. immuno profiling of an individual's immune response focused on one antigen, it is preferred that all possible binding peptides of that antigen are included in the application (i.e. the "total approach" for the design of binding peptides described below). For other applications, e.g vaccines it may be adequate to include a few or just one binding peptide for each of the HLA-alleles included in the application (i.e. the "directed approach" whereby only the most potent binding peptides can be included). Personalized diagnostics, therapeutics and vaccines will often fall in- between these two extremes, as it will only be necessary to include a few or just one binding peptide in e.g. a vaccine targeting a given individual, but the specific binding peptide may have to be picked from binding peptides designed by the total approach, and identified through the use of immuno profiling studies involving all possible binding peptides. The principles of immuno profiling is described elsewhere herein.

a) Total approach

The MHC class 1 binding peptide prediction is done as follows using the total approach. The actual protein sequence is split up into 8-, 9-, 10-, and 1 1 -mer peptide sequences. This is performed by starting at amino acid position 1 identifying the first 8- mer; then move the start position by one amino acid identifying the second 8-mer; then move the start position by one amino acid, identifying the third 8-mer. This procedure continues by moving start position by one amino acid for each round of peptide identification. Generated peptides will be amino acid position 1 -8, 2-9, 3-10 etc. This procedure can be carried out manually or by means of a software program (Figure 2). This procedure is then repeated in an identical fashion for 9-, 10 and 1 1 -mers, respectively.

b) Directed approach

The directed approach identifies a preferred subset of binding peptides from the binding peptides generated in the total approach. This preferred subset is of particularly value in a given context. Software programs are available that use neural networks or established binding preferences to predict the interaction of specific binding peptides with specific MHC class I alleles, and/or probability of the binding peptide in question to be generated by the proteolytic machinery of the average individual. However, the proteolytic activitiy varies a lot among individuals, and for personalized diagnostics, treatment or vaccination it may be desirable to disregard these general proteolytic data. Examples of such programs are www.syfpeithi.de; www.imtech.res.in/raghava/propred1/index.html; www.cbs.dtu.dk/services/NetMHC/. Identified peptides can then be tested for biological relevance in functional assays such as Cytokine release assays, ELISPOT and CTL killing assays or their binding to selected MHC molecules may be determined in binding assays.

Prediction of good HLA class 1 peptide binders can be done at the HLA superfamily level even taking the combined action of endosolic, cytosolic and membrane bound protease activities as well as the TAP1 and TAP2 transporter specificities into consideration using the program www.cbs.dtu.dk/services/NetCTL/.

Alternatively, simple consensus sequences for the individual MHC allele can be used to choose a set of relevant binding peptides that will suit the "average" individual. Such consensus sequences often solely consider the affinity of the binding peptide for the MHC protein; in other words, a subset of binding peptides is identified where the designed binding peptides have a high probability of forming stable MHC-peptide complexes, but where it is uncertain whether this MHC-peptide complex is of high relevance in a population, and more uncertain whether this MHC-peptide complex is of high relevance in a given individual. For class I MHC-alleles, the consensus sequence for a binding peptide is generally given by the formula

X1 -X2-X3-X4-....-Xn, where n equals 8, 9, 10, or 1 1 , and where X represents one of the twenty naturally occurring amino acids, optionally modified as described elsewhere in this application. XI -Xn can be further defined. Thus, certain positions in the consensus sequence are the socalled anchor positions and the selection of useful amino acids for these positions is limited to those able to fit into the corresponding binding pockets in the HLA molecule. For HLA-A^*02, for example, X2 and X9 are primary anchor positions and useful amino acids at these two positions in the binding peptide are preferable limited to leucine or methionine for X2 and to valine or leucine at postion X9. In contrast the primary anchor positions of peptides binding HLA-B^*08 are X3, X5 and X9 and the corresponding preferred amino acids at these positions are lysine at position X3, lysine or arginine at position X5 and leucine at position X9.

Design of MHC class 2 binding peptide sequence. a) Total approach and b) directed approach The approach to predict putative peptide binders for MHC class 2 can be done in a similar way as described for MHC class 1 binding peptide prediction above. The change is the different size of the peptides, which is preferably 13-16 amino acids long for MHC class 2. The putative binding peptide sequences only describe the central part of the peptide including the 9-mer core peptide; in other words, the peptide sequences shown represent the core of the binding peptide with a few important flanking amino acids, which in some cases may be of considerably length generating binding peptides longer than the 13-16 amino acids.

Alternatively, simple consensus sequences for the individual MHC allele can be used to choose a set of relevant binding peptides that will suit the "average" individual. Such consensus sequences often solely consider the affinity of the binding peptide for the MHC protein; in other words, a subset of binding peptides is identified where the designed binding peptides have a high probability of forming stable MHC-peptide complexes, but where it is uncertain whether this MHC-peptide complex is of high relevance in a population, and more uncertain whether this MHC-peptide complex is of high relevance in a given individual.

For class Il MHC-alleles, the consensus sequence for the interacting core of a binding peptide is generally given by the formula X1 -X2-X3-X4-....-Xn, where n equals 9, and where X represents one of the twenty naturally occurring amino acids, optionally modified as described elsewhere in this application. XI -Xn can be further defined. Thus, certain positions in the consensus sequence are the socalled anchor positions and the selection of useful amino acids for these positions is limited to those able to fit into the corresponding binding pockets in the HLA molecule. For example HLA-DRB1 ^*1501 have X1 , X4 and X7 as primary anchor positions where preferred amino acids at the three positions are as follows, X1 : leucine, valine and isoleucine, X4: phenylalanine, tyrosine or isoleucine, X7: isoleucine, leucine, valine, methionine or phenylalanine. In general, MHC Il binding peptides have much more varied anchor positions than MHC I binding peptides and the number of usefull amino acids at each anchor position is much higher.

Choice of MHC allele

More than 600 MHC alleles (class 1 and 2) are known in humans; for many of these, the peptide binding characteristics are known. Figure 3 presents an updated list of the HLA class 1 alleles. The frequency of the different HLA alleles varies considerably, also between different ethnic groups (Figure 4). Thus it is of outmost importance to carefully select the MHC alleles that corresponds to the population that one wish to study.

The combined choice of peptide, MHC and carrier.

Above it has been described how to generate binding peptides, and which MHC alleles are available. Below it is further described how one may modify the binding peptides in order to increase the stability, affinity, specificity and other features of the MHC-peptide complex or the MHC multimer. In the following it is described what characteristics of binding peptides and MHC alleles are important when using the MHC-peptide complex or MHC-multimer for different purposes.

A first preferred embodiment employs binding peptides of particularly high affinity for the MHC proteins. This may be done in order to increase the stability of the MHC- peptide complex. A higher affinity of the binding peptide for the MHC proteins may in some instances also result in increased rigidity of the MHC-peptide complex, which in turn often will result in higher affinity and/or specificity of the MHC-peptide complex for the T-cell receptor. A higher affinity and specificity will in turn have consequences for the immunogenicity and allergenicity, as well as possible side-effects of the MHC- peptide complex in e.g. the body.

Binding peptides of particularly high affinity for the MHC proteins may be identified by several means, including the following.

• Incubation of candidate binding peptides and MHC proteins, followed by analysis of the resulting complexes to identify those binding peptides that have most frequently been associated with MHC proteins. The binding peptides that have most frequently been associated with MHC proteins typically will represent high-affinity binding peptides. The identification of binding peptides with particularly high-affinity may involve enrichment of binding peptides, e.g. incubation of candidate peptides with immobilized MHC molecules, removal of non-binding peptides by e.g. washing, elution of binding peptides. This pool of peptides enriched for binding to the chosen MHC molecules may then be identified e.g. by mass spectrometry or HPLC and amino acid sequencing or the pool can be further enriched by another round of incubation with immobilized MHC. O

• Candidate binding peptides may be compared to consensus sequences for the binding to a specific MHC allele. Thus, for a given class 1 allele, the consensus 8'mer sequence may be given by the sequence "X1 -X2-X3-X4-X5-X6-X7-X8", where each of the X1 -X8 amino acids can be chosen from a specific subset of amino acids, as described above.

Those binding peptides that correlate the best with the consensus sequence are expected to have particularly high affinity for the MHC allele in question.

• Based on a large data set of affinities of binding peptides for specific MHC alleles, software programs (often involving neural networks) have been developed that allow a relatively accurate prediction of the affinity of a given candidate binding peptide for a given MHC allele. By examining candidate binding peptides using such software programs, one can identify binding peptides of expected high-affinity for the MHC molecule.

A second prefered embodiment employs binding peptides with medium affinity for the MHC molecule. A medium affinity of the peptide for the MHC protein will often lead to lower physical and chemical stability of the MHC-peptide complex, which can be an advantage for certain applications. As an example, it is often desirable to administer a drug on a daily basis due to convenience. An MHC-peptide complex-based drug with high stability in the body would not allow this. In contrast a binding peptide with medium or low affinity for the MHC protein can be an advantage for such applications, since these functional MHC-peptide molecules will be cleared more rapidly from the body due to their lower stability.

For some applications where some level of cross-talk is desired, e.g. in applications where the target is a number of T cell clones that interact with a number of structurally related MHC-peptide complexes, e.g. MHC-peptide complexes containing binding peptides from different strains of a given species, a medium or low affinity of the binding peptide for the MHC protein can be an advantage. Thus, these MHC-peptide complexes are often more structurally flexible, allowing the MHC-peptide complexes to interact with several structurally related TCRs.

The affinity of a given peptide for a MHC protein, predicted by a software program or by its similarity to a consensus sequence, should only be considered a guideline to its real affinity. Moreover, the affinity can vary a lot depending on the conditions in the o

environment, e.g. the affinity in blood may be very different from the affinity in a biochemical assay. Further, in the context of a MHC multimer, the flexibility of the MHC-peptide complex can sometimes be an important parameter for overall avidity.

In summary, a lot of factors must be considered for the choice of binding peptides in a certain application. Some applications benefit from the use of all possible binding peptides for an antigen ("total approach"), other applications benefit from the selective choice of just a few binding peptides. Depending on the application, the affinity of the binding peptide for MHC protein is preferably high, medium, or low; the physical and/or chemical stability of the MHC-peptide complex is preferably high, medium or low; the binding peptide is preferably a very common or very rare epitope in a given population; etc.

It is obvious from the above preferred embodiments that most or all of the binding peptides generated by the total approach have important applications. In other words, in order to make relevant MHC multimers that suit the different applications with regard to e.g. personalized or general targeting, or with regard to affinity, avidity, specificity, immunogenicity, stimulatory efficiency, or stability, one must be able to choose from the whole set of binding peptides generated by the total approach

Peptide modifications

In addition to the binding peptides designed by the total approach, homologous peptides and peptides that have been modified in the amino acid side chains or in the backbone can be used as binding peptides.

Homologous peptides

Homologues MHC peptide sequences may arise from the existence of multiple strongly homologous alleles, from small insertions, deletions, inversions or substitutions. If they are sufficiently homologous to peptides derived by the total approach, i.e. have an amino acid sequence identity greater than e.g. more than 90%, more than 80%, or more than 70%, or more than 60%, to one or two binding peptides derived by the total approach, they may be good candidates. Identity is often most important for the anchor residues. A MHC binding peptide may be of split- or combinatorial epitope origin i.e. formed by linkage of peptide fragments derived from two different peptide fragments and/or proteins. Such peptides can be the result of either genetic recombination on the DNA level or due to peptide fragment association during the complex break down of proteins during protein turnover. Possibly it could also be the result of faulty reactions during protein synthesis i.e. caused by some kind of mixed RNA handling. A kind of combinatorial peptide epitope can also be seen if a portion of a longer peptide make a loop out leaving only the terminal parts of the peptide bound in the groove.

Uncommon, artificial and chemically modified amino acids.

Peptides having un-common amino acids, such as selenocysteine and pyrrolysine, may be bound in the MHC groove as well. Artificial amino acids e.g. having the isomeric D-form may also make up isomeric D-peptides that can bind in the binding groove of the MHC molecules. Bound peptides may also contain amino acids that are chemically modified or being linked to reactive groups that can be activated to induce changes in or disrupt the peptide. Example post-translational modifications are shown below. However, chemical modifications of amino acid side chains or the peptide backbone can also be performed.

Any of the modifications can be found individually or in combination at any position of the peptide, e.g. position 1 , 2, 3, 4, 5, 6, etc. up to n.

Phosphorylation, Sulfation, Porphyrin ring linkage, Flavin linkage

Tyrosine GFP prosthetic group (Thr-Tyr-Gly sequence) formation, Lysine tyrosine quinone (LTQ) formation, Topaquinone (TPQ) formation

Asparagine Deamidation, Glycosylation

Aspartate Succinimide formation

Glutamine Transglutamination

Glutamate Carboxylation, Methylation, Polyglutamylation, Polyglycylation

Arginine Citrullination, Methylation

Proline Hydroxylation

Table 1 Post translational modification of peptides

Post translationallv modified peptides

The amino acids of the antigenic peptides can also be modified in various ways dependent on the amino acid in question, or the modification can affect the amino- or carboxy-terminal end of the peptide. See table 1. Such peptide modifications are occuring naturally as the result of post tranlational processing of the parental protein. A non-exhaustive description of the major post translational modifications is given below, divided into three main types.

a) Modification that adds a chemical moiety to the binding peptide.

acetylation, the addition of an acetyl group, usually at the N-terminus of the protein

alkylation, the addition of an alkyl group (e.g. methyl, ethyl). Methylation, the addition of a methyl group, usually at lysine or arginine residues is a type of alkylation. Demethylation involves the removal of a methyl-group.

amidation at C-terminus

biotinylation, acylation of conserved lysine residues with a biotin appendage

formylation

gamma-carboxylation dependent on Vitamin K

glutamylation, covalent linkage of glutamic acid residues to tubulin and some other proteins by means of tubulin polyglutamylase

glycosylation, the addition of a glycosyl group to either asparagine, hydroxylysine, serine, or threonine, resulting in a glycoprotein. Distinct from glycation, which is regarded as a nonenzymatic attachment of sugars.

glycylation, covalent linkage of one to more than 40 glycine residues to the tubulin C-terminal tail

heme moiety may be covalently attached hydroxylation, is any chemical process that introduces one or more hydroxyl groups (-OH) into a compound (or radical) thereby oxidizing it. The principal residue to be hydroxylated is Proline. The hydroxilation occurs at the C^γ atom, forming hydroxyproline (Hyp). In some cases, proline may be hydroxylated instead on its C^β atom. Lysine may also be hydroxylated on its C^δ atom, forming hydroxylysine (HyI).

iodination

isoprenylation, the addition of an isoprenoid group (e.g. farnesol and geranylgeraniol)

lipoylation, attachment of a lipoate functionality, as in prenylation, GPI anchor formation, myristoylation, farnesylation, geranylation

nucleotides or derivatives thereof may be covalently attached, as in ADP- ribosylation and flavin attachment

oxidation, lysine can be oxidized to aldehyde

pegylation, addition of poly-ethylen-glycol groups to a protein. Typical reactive amino acids include lysine, cysteine, histidine, arginine, aspartic acid, glutamic acid, serine, threonine, tyrosine. The N-terminal amino group and the C-terminal carboxylic acid can also be used

phosphatidylinositol may be covalently attached

phosphopantetheinylation, the addition of a 4'-phosphopantetheinyl moiety from coenzyme A, as in fatty acid, polyketide, non-ribosomal peptide and leucine biosynthesis

phosphorylation, the addition of a phosphate group, usually to serine, tyrosine, threonine or histidine

pyroglutamate formation as a result of N-terminal glutamine self-attack, resulting in formation of a cyclic pyroglutamate group.

racemization of proline by prolyl isomerase tRNA-mediated addition of amino acids such as arginylation

sulfation, the addition of a sulfate group to a tyrosine.

Selenoylation (co-translational incorporation of selenium in selenoproteins)

b) Modification that adds protein or peptide.

ISGylation, the covalent linkage to the ISG15 protein (Interferon-Stimulated Gene

15)

SUMOylation, the covalent linkage to the SUMO protein (Small Ubiquitin-related Modifier)

ubiquitination, the covalent linkage to the protein ubiquitin.

c) Modification that converts one or more amino acids to different amino acids.

citrullination, or deimination the conversion of arginine to citrulline

deamidation, the conversion of glutamine to glutamic acid or asparagine to aspartic acid

The peptide modifications can occur as modification of a single amino acid or more than one i.e. in combinations. Modifications can be present on any position within the peptide i.e. on position 1 , 2, 3, 4, 5, etc. for the entire length of the peptide.

Sources of binding peptides a) From natural sources The binding peptides can be obtained from natural sources by enzymatic digestion or proteolysis of natural proteins or proteins derived by in vitro translation of mRNA. Binding peptides may also be eluted from the MHC binding groove.

b) From recombinant sources 1) as monomeric or multimeric peptide

Alternatively peptides can be produced recombinantly by transfected cells either as monomeric antigenic peptides or as multimeric (concatemeric) antigenic peptides. Optionally, the Multimeric antigenic peptides are cleaved to form monomeric antigenic peptides before binding to MHC protein.

2) as part of a bigger recombinant protein

Binding peptides may also constitute a part of a bigger recombinant protein e.g. consisting of,

2a) For MHC class 1 binding peptides, Peptide-linker-β2m, β2m being full length or truncated;

Peptide-linker-MHC class 1 heavy chain, the heavy chain being full length or truncated. Most importantly the truncated class I heavy chain will consist of the extracellular part i.e the αi , α2, and α domains. The heavy chain fragment may also only contain the α1 and α2 domains, or α1 domain alone, or any fragment or full length β2m or heavy chain attached to a designer domain(s) or protein fragment(s).

2b) For MHC class 2 binding peptides the recombinant construction can consist of,

Peptide-linker-MHC class 2 α-chain, full length or truncated;

Peptide-linker-MHC class 2 β-chain, full length or truncated;

Peptide-linker-MHC class 2 α-chain-linker-MHC class 2 β-chain, both chains can be full length or truncated, truncation may involve, omission of α- and/or β-chain intermembrane domain, or omission of α- and/or β-chain intermembrane plus cytoplasmic domains. MHC class 2 part of the construction may consist of fused domains from NH2-terminal, MHC class 2 β1 domain-MHC class 2 α1 domain-constant oc3 of MHC class 1 , or alternatively of fused domains from NH2-terminal, MHC class 2 α1 domain-MHC class 2 β1 domain-constant α3 of MHC class 1 . In both cases β2m will be associated non-covalently in the folded MHC complex. β2m can also be covalently associated in the folded MHC class 2 complex if the following constructs are used from NH2 terminal, MHC class 2 β1domain-MHC class 2 α1 domain-constant α3 of MHC class 1 -linker-β2m, or alternatively of fused domains from NH2-terminal, MHC class 2 α1 domain-MHC class 2 β1 domain-constant α3 of MHC class 1 -linker-β2m; the construct may also consist of any of the above MHC class 2 constructs with added designer domain(s) or sequence(s).

c) From chemical synthesis MHC binding peptide may also be chemically synthesized by solid phase or fluid phase synthesis, according to standard protocols.

Comprehensive collections of antigenic peptides, derived from one antigen, may be prepared by a modification of the solid phase synthesis protocol, as described in the following and exemplified in Example 21 .

The protocol for the synthesis of the full-length antigen on solid support is modified by adding a partial cleavage step after each coupling of an amino acid. Thus, the starting point for the synthesis is a solid support to which has been attached a cleavable linker. Then the first amino acid X1 (corresponding to the C-terminal end of the antigen) is added and a coupling reaction performed. The solid support now carries the molecule "Iinker-X1 ". After washing, a fraction (e.g. 10%) of the cleavable linkers are now cleaved, to release into solution X1. The supernatant is transferred to a collection container. Additional solid support carrying a cleavable linker is added, e.g. corresponding to 10% of the initial amount of solid support.

Then the second amino acid X2 is added and coupled to X1 or the cleavable linker, to form on solid support the molecules "Iinker-X2" and "Iinker-X1 -X2". After washing, a fraction (e.g. 10%) of the cleavable linker is cleaved, to release into solution X2 and X1 -X2. The supernatant is collected into the collection container, which therefore now contains X1 , X2, and X1 -X2. Additional solid support carrying a cleavable linker is added, e.g. corresponding to 10% of the initial amount of solid support. Then the third amino acid X3 is added and coupled to X2 or the cleavable linker, to form on solid support the molecules "Iinker-X3", "Iinker-X2-X3" and "Iinker-X1 -X2-X3". After washing, a fraction (e.g. 10%) of the cleavable linker is cleaved, to release into solution X3, X2-X3 and X1 -X2-X3. The supernatant is collected into the collection container, which therefore now contains X1 , X2, X3, X1 -X2, X2-X3 and X1 -X2-X3. Additional solid support carrying a cleavable linker is added, e.g. corresponding to 10% of the initial amount of solid support. This step-wise coupling and partial cleavage of the linker is continued until the N- terminal end of the antigen is reached. The collection container will now contain a large number of peptides of different length and sequence. In the present example where a 10% partial cleavage was employed, a large fraction of the peptides will be 8'-mers, 9'- mers, 10'-mers and 1 1 '-mers, corresponding to class I antigenic peptides. As an example, for a 100 amino acid antigen the 8'-mers will consist of the sequences X1 -X2- X3-X4-X5-X6-X7-X8, X2-X3-X4-X5-X6-X7-X8-X9, , X93-X94-X95-X96-X97-X98-

X99-X100.

Optionally, after a number of coupling and cleavage steps or after each coupling and cleavage step, the used (inactivated) linkers on solid support can be regenerated, in order to maintain a high fraction of linkers available for synthesis. The collection of antigenic peptides can be used as a pool for e.g. the display by APCs to stimulate CTLs in ELISPOT assays, or the antigenic peptides may be mixed with one or more MHC alleles, to form a large number of different MHC-peptide complexes which can e.g. be used to form a large number of different MHC multimers which can e.g. be used in flow cytometry experiments.

Loading of the peptide into the MHCmer

Loading of the peptides into the MHCmer being either MHC class 1 or class 2 can be performed in a number of ways depending on the source of the peptide and the MHC, and depending on the application. MHC class 2 molecules can in principle be loaded with peptides in similar ways as MHC class 1. However, due to complex instability the most successful approach have been to make the complexes recombinant in toto in eukaryotic cells from a gene construct encoding the following form β chain-flexible linker-α chain-flexible linker-antigenic peptide.

The antigenic peptide may be added to the other peptide chain(s) at different times and in different forms, as follows. a) Loading of antigenic peptide during MHC complex folding a1) Antigenic peptide is added as a free peptide MHC class I molecules are most often loaded with peptide during assembly in vitro by the individual components in a folding reaction i.e. consisting of purified recombinant heavy chain α with the purified recombinant β2 microglobulin and a peptide or a peptide mix. a2) Antigenic peptide is part of a recombinant protein construct Alternatively the peptide to be folded into the binding groove can be encoded together with e.g. the α heavy chain or fragment hereof by a gene construct having the structure, heavy chain-flexible linker- peptide. This recombinant molecule is then folded in vitro with β2-microglobulin.

b) Antigenic peptide replaces another antigenic peptide by an exchange reaction. b1) Exchange reaction "in solution"

Loading of desired peptide can also be made by an in vitro exchange reaction where a peptide already in place in the binding groove are being exchanged by another peptide species. b2) Exchange reaction "in situ"

Peptide exchange reactions can also take place when the parent molecule is attached to other molecules, structures, surfaces, artificial or natural membranes and nano- particles. b3) Aided exchange reaction. This method can be refined by making the parent construct with a peptide containing a meta-stable amino acid analog that is split by either light or chemically induction thereby leaving the parent structure free for access of the desired peptide in the binding groove. b4) Display by in vivo loading Loading of MHC class I and Il molecules expressed on the cell surface with the desired peptides can be performed by an exchange reaction. Alternatively cells can be transfected by the peptides themselves or by the mother proteins that are then being processed leading to an in vivo analogous situation where the peptides are bound in the groove during the natural cause of MHC expression by the transfected cells. In the case of professional antigen presenting cells e.g. dendritic cells, macrophages,

Langerhans cells, the proteins and peptides can be taken up by the cells themselves by phagocytosis and then bound to the MHC complexes the natural way and expressed on the cell surface in the correct MHC context.

Other features of product

In one preferred embodiment the MHC multimer is between 50,000 Da and 1 ,000,000 Da, such as from 50,000 Da to 980,000; for example from 50,000 Da to 960,000; such as from 50,000 Da to 940,000; for example from 50,000 Da to 920,000; such as from 50,000 Da to 900,000; for example from 50,000 Da to 880,000; such as from 50,000 Da to 860,000; for example from 50,000 Da to 840,000; such as from 50,000 Da to

820,000; for example from 50,000 Da to 800,000; such as from 50,000 Da to 780,000; for example from 50,000 Da to 760,000; such as from 50,000 Da to 740,000; for example from 50,000 Da to 720,000; such as from 50,000 Da to 700,000; for example from 50,000 Da to 680,000; such as from 50,000 Da to 660,000; for example from 50,000 Da to 640,000; such as from 50,000 Da to 620,000; for example from 50,000 Da to 600,000; such as from 50,000 Da to 580,000; for example from 50,000 Da to 560,000; such as from 50,000 Da to 540,000; for example from 50,000 Da to 520,000; such as from 50,000 Da to 500,000; for example from 50,000 Da to 480,000; such as from 50,000 Da to 460,000; for example from 50,000 Da to 440,000; such as from 50,000 Da to 420,000; for example from 50,000 Da to 400,000; such as from 50,000 Da to 380,000; for example from 50,000 Da to 360,000; such as from 50,000 Da to 340,000; for example from 50,000 Da to 320,000; such as from 50,000 Da to 300,000; for example from 50,000 Da to 280,000; such as from 50,000 Da to 260,000; for example from 50,000 Da to 240,000; such as from 50,000 Da to 220,000; for example from 50,000 Da to 200,000; such as from 50,000 Da to 180,000; for example from 50,000 Da to 160,000; such as from 50,000 Da to 140,000; for example from 50,000 Da to 120,000; such as from 50,000 Da to 100,000; for example from 50,000 Da to 80,000; such as from 50,000 Da to 60,000; such as from 100,000 Da to 980,000; for example from 100,000 Da to 960,000; such as from 100,000 Da to 940,000; for example from 100,000 Da to 920,000; such as from 100,000 Da to 900,000; for example from 100,000 Da to 880,000; such as from 100,000 Da to 860,000; for example from 100,000 Da to 840,000; such as from 100,000 Da to 820,000; for example from 100,000 Da to 800,000; such as from 100,000 Da to 780,000; for example from 100,000 Da to 760,000; such as from 100,000 Da to 740,000; for example from 100,000 Da to 720,000; such as from 100,000 Da to 700,000; for example from 100,000 Da to 680,000; such as from 100,000 Da to 660,000; for example from 100,000 Da to 640,000; such as from 100,000 Da to 620,000; for example from 100,000 Da to 600,000; such as from 100,000 Da to 580,000; for example from 100,000 Da to 560,000; such as from 100,000 Da to 540,000; for example from 100,000 Da to 520,000; such as from 100,000 Da to 500,000; for example from 100,000 Da to 480,000; such as from 100,000 Da to 460,000; for example from 100,000 Da to 440,000; such as from 100,000 Da to 420,000; for example from 100,000 Da to 400,000; such as from 100,000 Da to 380,000; for example from 100,000 Da to 360,000; such as from 100,000 Da to 340,000; for example from 100,000 Da to 320,000; such as from 100,000 Da to 300,000; for example from 100,000 Da to 280,000; such as from 100,000 Da to 260,000; for example from 100,000 Da to 240,000; such as from 100,000 Da to 220,000; for example from 100,000 Da to 200,000; such as from 100,000 Da to 180,000; for example from 100,000 Da to 160,000; such as from 100,000 Da to 140,000; for example from 100,000 Da to 120,000; such as from 150,000 Da to 980,000; for example from 150,000 Da to 960,000; such as from 150,000 Da to 940,000; for example from 150,000 Da to 920,000; such as from 150,000 Da to 900,000; for example from 150,000 Da to 880,000; such as from 150,000 Da to 860,000; for example from 150,000 Da to 840,000; such as from 150,000 Da to 820,000; for example from 150,000 Da to 800,000; such as from 150,000 Da to 780,000; for example from 150,000 Da to 760,000; such as from 150,000 Da to 740,000; for example from 150,000 Da to 720,000; such as from 150,000 Da to 700,000; for example from 150,000 Da to 680,000; such as from 150,000 Da to 660,000; for example from 150,000 Da to 640,000; such as from 150,000 Da to 620,000; for example from 150,000 Da to 600,000; such as from 150,000 Da to 580,000; for example from 150,000 Da to 560,000; such as from 150,000 Da to 540,000; for example from 150,000 Da to 520,000; such as from 150,000 Da to 500,000; for example from 150,000 Da to 480,000; such as from 150,000 Da to 460,000; for example from 150,000 Da to 440,000; such as from 150,000 Da to 420,000; for example from 150,000 Da to 400,000; such as from 150,000 Da to 380,000; for example from 150,000 Da to 360,000; such as from 150,000 Da to 340,000; for example from 150,000 Da to 320,000; such as from 150,000 Da to 300,000; for example from 150,000 Da to 280,000; such as from 150,000 Da to 260,000; for example from 150,000 Da to 240,000; such as from 150,000 Da to 220,000; for example from 150,000 Da to 200,000; such as from 150,000 Da to 180,000; for example from 150,000 Da to 160,000.

In another preferred embodiment the MHC multimer is between 1 ,000,000 Da and 3,000,000 Da, such as from 1 ,000,000 Da to 2,800,000; for example from 1 ,000,000 Da to 2,600,000; such as from 1 ,000,000 Da to 2,400,000; for example from 1 ,000,000 Da to 2,200,000; such as from 1 ,000,000 Da to 2,000,000; for example from 1 ,000,000 Da to 1 ,800,000; such as from 1 ,000,000 Da to 1 ,600,000; for example from 1 ,000,000 Da to 1 ,400,000.

Above it was described how to design and produce the key components of the MHC multimers, i.e. the MHC-peptide complex. In the following it is described how to generate the MHC monomer or MHC multimer products of the present invention. Number of MHC complexes pr multimer

A non-exhaustive list of possible MHC mono- and multimers illustrates the possibilities, n indicates the number of MHC complexes comprised in the multimer:

a) n=1 , Monomers

b) n=2, Dimers, multimerization can be based on IgG scaffold, streptavidin with two MHCs, coiled-coil dimerization e.g. Fos.Jun dimerization

c) n=3, Trimers, multimerization can be based on streptavidin as scaffold with three MHCs, TNFalpha-MHC hybrids, triplex DNA-MHC konjugates or other trimer structures

d) n=4, Tetramers, multimerization can be based on streptavidin with all four binding sites occupied by MHC molecules or based on dimeric IgA

e) n=5, Pentamers, multimerization can take place around a pentameric coil-coil structure

f) n=6, Hexamers

g) n=7, Heptamers

h) n=8-12, Octa- dodecamers, multimerization can take place using Streptactin

i) n=10, Decamers, multimerization can take place using IgM

j) 1 <n<100, Dextramers, as multimerization domain polymers such as polypeptide, polysaccharides and Dextrans can be used.

k) 1 <n<1000, Multimerization can make use of dendritic cells (DC), antigen-presenting cells (APC), micelles, liposomes, beads, surfaces e.g. microtiterplate, tubes, microarray devices, micro-fluidic systems I) 1 <n, n in billions or trillions or higher, multimerization take place on beads, and surfaces e.g. microtiterplate, tubes, microarray devices, micro-fluidic systems

MHC origin

Any of the three components of a MHC complex can be of any of the below mentioned origins. The list is non- exhaustive. A complete list would encompass all Chordate species. By origin is meant that the sequence is identical or highly homologous to a naturally occurring sequence of the specific species.

List of origins:

• Human

• Mouse

• Primate o Chimpansee o Gorilla o Orang Utan

• Monkey o Macaques • Porcine (Swine/Pig)

• Bovine (Cattle/Antilopes)

• Equine (Horse)

• Camelides (Camels)

• Ruminants (Deears) • Canine (Dog)

• Feline (Cat)

• Bird o Chicken o Turkey • Fish

• Reptiles

• Amphibians

Generation of MHC multimers Different approaches to the generation of various types of MHC multimers are described in US patent 5,635,363 (Altmann et al.), patent application WO 02/072631 A2 (Winther et al.), patent application WO 99/42597, US patent 2004209295, US patent 5,635,363, and is described elsewhere in the present patent application as well. In brief, MHC multimers can be generated by first expressing and purifying the individual protein components of the MHC protein, and then combining the MHC protein components and the peptide, to form the MHC-peptide complex. Then an appropriate number of MHC-peptide complexes are linked together by covalent or non- covalent bonds to a multimerization domain. This can be done by chemical reactions between reactive groups of the multimerization domain (e.g. vinyl sulfone functionalities on a dextran polymer) and reactive groups on the MHC protein (e.g. amino groups on the protein surface), or by non-covalent interaction between a part of the MHC protein (e.g. a biotinylated peptide component) and the multimerization domain (e.g. four binding sites for biotin on the strepavidin tetrameric protein). As an alternative, the MHC multimer can be formed by the non-covalent association of amino acid helices fused to one component of the MHC protein, to form a pentameric MHC multimer, held together by five helices in a coiled-coil structure making up the multimerization domain.

Appropriate chemical reactions for the covalent coupling of MHC and the multimerization domain include nucleophilic substitution by activation of electrophiles

(e.g. acylation such as amide formation, pyrazolone formation, isoxazolone formation; alkylation; vinylation; disulfide formation), addition to carbon-hetero multiple bonds (e.g. alkene formation by reaction of phosphonates with aldehydes or ketones; arylation; alkylation of arenes/hetarenes by reaction with alkyl boronates or enolethers), nucleophilic substitution using activation of nucleophiles (e.g. condensations; alkylation of aliphatic halides or tosylates with enolethers or enamines), and cycloadditions.

Appropriate molecules, capable of providing non-covalent interactions between the multimerization domain and the MHC-peptide complex, involve the following molecule pairs and molecules: streptavidin/biotin, avidin/biotin, antibody/antigen, DNA/DNA,

DNA/PNA, DNA/RNA, PNA/PNA, LNA/DNA, leucine zipper e.g. Fos/Jun, IgG dimeric protein, IgM multivalent protein, acid/base coiled-coil helices, chelate/metal ion-bound chelate, streptavidin (SA) and avidin and derivatives thereof, biotin, immunoglobulins, antibodies (monoclonal, polyclonal, and recombinant), antibody fragments and derivatives thereof, leucine zipper domain of AP-1 (jun and fos), hexa-his (metal chelate moiety), hexa-hat GST (glutathione S-transferase) glutathione affinity, Calmodulin-binding peptide (CBP), Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, E2Tag, HA Epitope Tag, Myc Epitope, FLAG Epitope, AU1 and AU5 Epitopes, GIu-GIu Epitope, KT3 Epitope, IRS Epitope, Btag Epitope, Protein Kinase-C Epitope, VSV Epitope, lectins that mediate binding to a diversity of compounds, including carbohydrates, lipids and proteins, e.g. Con A (Canavalia ensiformis) or WGA (wheat germ agglutinin) and tetranectin or Protein A or G (antibody affinity). Combinations of such binding entities are also comprised. In particular, when the MHC complex is tagged, the binding entity can be an "anti-tag". By "anti-tag" is meant an antibody binding to the tag and any other molecule capable of binding to such tag.

Generation of components of MHC

When employing MHC multimers for diagnostic purposes, it is preferable to use a MHC allele that corresponds to the tissue type of the person or animal to be diagnosed. Once the MHC allele has been chosen, a peptide derived from the antigenic protein may be chosen. The choice will depend on factors such as known or expected binding affinity of the MHC protein and the various possible peptide fragments that may be derived from the full sequence of the antigenic peptide, and will depend on the expected or known binding affinity and specificity of the MHC-peptide complex for the TCR. Preferably, the affinity of the peptide for the MHC molecule, and the affinity and specificity of the MHC-peptide complex for the TCR, should be high.

Similar considerations apply to the choice of MHC allele and peptide for therapeutic and vaccine purposes. In addition, for some of these applications the effect of binding the MHC multimer to the TCR is also important. Thus, in these cases the effect on the T-cell's general state must be considered, e.g. it must be decided whether the desired end result is apoptosis or proliferation of the T-cell.

Likewise, it must be decided whether stability is important. For some applications low stability may be an advantage, e.g. when a short-term effect is desired; in other instances, a long-term effect is desired and MHC multimers of high stability is desired. Stabilities of the MHC protein and of the MHC-peptide complex may be modified as described elsewhere herein. Finally, modifications to the protein structure may be advantageous for some diagnostics purposes, because of e.g. increased stability, while for vaccine purposes modifications to the MHC protein structure may induce undesired allergenic responses.

Generation of protein chains of MHC

Generation of MHC class I heavy chain and β2-Microqlobulin

MHC class I heavy chain (HC) and β2-mircroglobulin (β2m) can be obtained from a variety of sources. a) Natural sources by means of purification from eukaryotic cells naturally expressing the MHC class 1 or β2m molecules in question. b) The molecules can be obtained by recombinant means e.g. using. a. in vitro translation of mRNA obtained from cells naturally expressing the MHC or β2m molecules in question b. by expression and purification of HC and/or β2m gene transfected cells of mammalian, yeast, bacterial or other origin. This last method will normally be the method of choice. The genetic material used for transfection/transformation can be: i. of natural origin isolated from cells, tissue or organisms ii. of synthetical origin i.e. synthetic genes identical to the natural DNA sequence or it could be modified to introduce molecular changes or to ease recombinant expression. The genetic material can encode all or only a fragment of β2m, all or only a fragment of MHC class 1 heavy chain. Of special interest are MHC class 1 heavy chain fragments consisting of, the complete chain minus the intramembrane domain, a chain consisting of only the extracellular α1 and α2 class 1 heavy chain domains, or any of the mentioned β2m and heavy chain fragments containing modified or added designer domain(s) or sequence(s).

Generation of MHC class 2 α- and β-chains

MHC class 2 α- and β-chains can be obtained from a variety of sources: a) Natural sources by means of purification from eukaryotic cells naturally expressing the MHC class 2 molecules in question. b) By recombinant means e.g. using: a. in vitro translation of mRNA obtained from cells naturally expressing the MHC class 2 molecules in question b. By purification from MHC class 2 gene transfected cells of mammalian, yeast, bacterial or other origin. This last method will normally be the method of choice.The genetic material used for transfection/transformation can be i. of natural origin isolated from cells, tissue or organisms ii. of synthetical origin i.e. synthetic genes identical to the natural

DNA sequence or it could be modified to introduce molecular changes or to ease recombinant expression.

The genetic material can encode all or only a fragment of MHC class 2 α- and β-chains. Of special interest are MHC class 2 α- and β-chain_fragments consisting of, the complete α- and 3-chains minus the intramembrane domains of either or both chains; and o> and 3-chains consisting of only the extracellular domains of either or both, i.e α1 plus α2 and β1 plus β2 domains, respectively. The genetic material can be modified to encode the interesting MHC class 2 molecule fragments consisting of domains starting from the amino terminal in consecutive order, MHC class 2 β1 plus MHC class 2 α1 plus MHC class 1 α3 domains or in alternative order, MHC class 2 α1 plus MHC class 2 β1 plus MHC class 1 α3 domains.

Lastly, the gentic material can encode any of the above mentioned MHC class 2 α- and β-chain molecules or fragments containing modified or added designer domain(s) or sequence(s). c) The MHC material may also be of exclusively synthetic origin manufactured by solid phase protein synthesis. Any of the above mentioned molecules can be made this way.

Modified MHC I or MHC Il complexes

MHC I and MHC Il complexes modified in any way as described above, can bind TCR. Modifications include mutations (substitutions, deletions or insertions of natural or non- natural amino acids, or any other organic molecule. The mutations are not limited to those that increase the stability of the MHC complex, and could be introduced anywhere in the MHC complex. One example of special interest is mutations introduced in the α3 subunit of MHC I heavy chain. The α3-subunit interacts with CD8 molecules on the surface of T cells. To minimize binding of MHC multimer to CD8 molecules on the surface of non-specific T cells, amino acids in α3 domain involved in the interaction with CD8 can be mutated. Such a mutation can result in altered or abrogated binding of MHC to CD8 molecules. Another example of special interest is mutations in areas of the β2-domain of MHC Il molecules responsible for binding CD4 molecules.

Another embodiment is chemically modified MHC complexes where the chemical modification could be introduced anywhere in the complex, e.g. a MHC complex where the peptide in the peptide-binding cleft has a dinitrophenyl group attached. Modified MHC complexes could also be MHC I or MHC Il fusion proteins where the fusion protein is not necessarily more stable than the native protein. Of special interest is MHC complexes fused with genes encoding an amino acid sequence capable of being biotinylated with a Bir A enzyme (Schatz, P. J. ,(1993), Biotechnology

1 1 (10):1 138-1 143). This biotinylation sequence could be fused with the COOH-terminal of β2m or the heavy chain of MHC I molecules or the COOH-terminal of either the α- chain or β-chain of MHC II. Similarly, other sequences capable of being enzymatically or chemically modified, can be fused to the NH₂ or COOH-terminal ends of the MHC complex.

Stabilization of empty MHC complexes and MHC-peptide complexes.

Classical MHC complexes are in nature embedded in the membrane. A preferred embodiment includes multimers comprising a soluble form of MHC Il or I where the transmembrane and cytosolic domains of the membrane-anchored MHC complexes are removed. The removal of the membrane-anchoring parts of the molecules can influence the stability of the MHC complexes. The stability of MHC complexes is an important parameter when generating and using MHC multimers.

MHC I complexes consist of a single membrane-anchored heavy chain that contains the complete peptide binding groove and is stable in the soluble form when complexed with β2m. The long-term stability is dependent on the binding of peptide in the peptide- binding groove. Without a peptide in the peptide binding groove the heavy chain and β2m tend to dissociate. Similarly, peptides with high affinity for binding in the peptide- binding groove will typically stabilize the soluble form of the MHC complex while peptides with low affinity for the peptide-binding groove will typically have a smaller stabilizing effect.

In contrast, MHC Il complexes consist of two membrane-anchored chains of almost equal size. When not attached to the cell membrane the two chains tend to dissociate and are therefore not stable in the soluble form unless a high affinity peptide is bound in the peptide-binding groove or the two chains are held together in another way.

In nature MHC I molecules consist of a heavy chain combined with β2m, and a peptide of typically 8-1 1 amino acids. Herein, MHC I molecules also include molecules consisting of a heavy chain and β2m (empty MHC), or a heavy chain combined with a peptide or a truncated heavy chain comprising α1 and α2 subunits combined with a peptide, or a full-length or truncated heavy chain combined with a full-length or truncated β2m chain. These MHC I molecules can be produced in E. coli as recombinant proteins, purified and refolded in vitro (Garboczi et al., (1992), Proc. Natl. Acad. Sci. 89, 3429-33). Alternatively, insect cell systems or mammalian cell systems can be used. To produce stable MHC I complexes and thereby generate reliable MHC I multimers several strategies can be followed. Stabilization strategies for MHC I complexes are described in the following.

Stabilization strategies for MHC I complexes

o Generation of covalent protein -fusions.

MHC I molecules can be stabilized by introduction of one or more linkers between the individual components of the MHC I complex. This could be a complex consisting of a heavy chain fused with β2m through a linker and a soluble peptide, a heavy chain fused to β2m through a linker, a heavy chain /β2m dimer covalently linked to a peptide through a linker to either heavy chain or β2m, and where there can or can not be a linker between the heavy chain and β2m, a heavy chain fused to a peptide through a linker, or the α1 and α2 subunits of the heavy chain fused to a peptide through a linker. In all of these example protein-fusions, each of the heavy chain, β2m and the peptide can be truncated.

The linker could be a flexible linker, e.g. made of glycine and serine and e.g. between 5-20 residues long. The linker could also be rigid with a defined structure, e.g. made of amino acids like glutamate, alanine, lysine, and leucine creating e.g. a more rigid structure.

In heavy chain-β2m fusion proteins the COOH terminus of β2m can be covalently linked to the NH₂ terminus of the heavy chain, or the NH₂ terminus of β2m can be linked to the COOH terminus of the heavy chain. The fusion-protein can also comprise a β2m domain, or a truncated β2m domain, inserted into the heavy chain, to form a fusion- protein of the form "heavy chain (first part)-β2m-heavy chain (last part)". Likewise, the fusion-protein can comprise a heavy chain domain, or a truncated heavy chain, inserted into the β2m chain, to form a fusion-protein of the form

"β2m(first part)-heavy chain-β2m(last part)".

In peptide-β2m fusion proteins the COOH terminus of the peptide is preferable linked to the NH₂ terminus of β2m but the peptide can also be linked to the COOH terminal of β2m via its NH₂ terminus. In heavy chain-peptide fusion proteins it is preferred to fuse the NH₂ terminus of the heavy chain to the COOH terminus of the peptide, but the fusion can also be between the COOH terminus of the heavy chain and the NH₂ terminus of the peptide. In heavy chain-β2m-peptide fusion proteins the NH₂ terminus of the heavy chain can be fused to the COOH terminus of β2m and the NH₂ terminus of β2m can be fused to the COOH terminus of the peptide.

o Non-covalent stabilization by binding to an unnatural component Non-covalent binding of unnatural components to the MHC I complexes can lead to increased stability. The unnatural component can bind to both the heavy chain and the β2m, and in this way promote the assemble of the complex, and/or stabilize the formed complex. Alternatively, the unnatural component can bind to either β2m or heavy chain, and in this way stabilize the polypeptide in its correct conformation, and in this way increase the affinity of the heavy chain for β2m and/or peptide, or increase the affinity of β2m for peptide.

Here, unnatural components mean antibodies, peptides, aptamers or any other molecule with the ability to bind peptides stretches of the MHC complex. Antibody is here to be understood as truncated or full-length antibodies (of isotype IgG, IgM, IgA, IgE), Fab, scFv or bi-Fab fragments or diabodies.

An example of special interest is an antibody binding the MHC I molecule by interaction with the heavy chain as well as β2m. The antibody can be a bispecific antibody that binds with one arm to the heavy chain and the other arm to the β2m of the MHC complex. Alternatively the antibody can be monospecific, and bind at the interface between heavy chain and β2m.

Another example of special interest is an antibody binding the heavy chain but only when the heavy chain is correct folded. Correct folded is here a conformation where the MHC complex is able to bind and present peptide in such a way that a restricted T cell can recognize the MHC-peptide complex and be activated. This type of antibody can be an antibody like the one produced by the clone W6/32

(M0736 from Dako, Denmark) that recognizes a conformational epitope on intact human and some monkey MHC complexes containing β2m, heavy chain and peptide.

o Generation of modified proteins or protein components

One way to improve stability of a MHC I complex is to increase the affinity of the binding peptide for the MHC complex. This can be done by mutation/substitution of amino acids at relevant positions in the peptide, by chemical modifications of amino acids at relevant positions in the peptide or introduction by synthesis of non-natural amino acids at relevant positions in the peptide. Alternatively, mutations, chemical modifications, insertion of natural or non-natural amino acids or deletions could be introduced in the peptide binding cleft, i.e. in the binding pockets that accommodate peptide side chains responsible for anchoring the peptide to the peptide binding cleft. Moreover, reactive groups can be introduced into the antigenic peptide; before, during or upon binding of the peptide, the reactive groups can react with amino acid residues of the peptide binding cleft, thus covalently linking the peptide to the binding pocket. Mutations/substitutions, chemical modifications, insertion of natural or non-natural amino acids or deletions could also be introduced in the heavy chain and/or β2m at positions outside the peptide-binding cleft. By example, it has been shown that substitution of XX with YY in position nn of human β₂m enhance the biochemical stability of MHC Class I molecule complexes and thus may lead to more efficient antigen presentation of subdominant peptide epitopes.

A preferred embodiment is removal of "unwanted cysteine residues" in the heavy chain by mutation, chemical modification, amino acid exchange or deletion. "Unwanted cysteine residues" is here to be understood as cysteines not involved in the correct folding of the final MHC I molecule. The presence of cysteine not directly involved in the formation of correctly folded MHC I molecules can lead to formation of intra molecular disulfide bridges resulting in a non correct folded MHC complex during in vitro refolding.

Another method for covalent stabilization of MHC I complex am to covalently attach a linker between two of the subunits of the MHC complex. This can be a linker between peptide and heavy chain or between heavy chain and beta2microglobulin.

Stabilization with soluble additives.

The stability of proteins in aqueous solution depends on the composition of the solution. Addition of salts, detergents organic solvent, polymers ect. can influence the stability. Of special interest are additives that increase surface tension of the MHC molecule without binding the molecule. Examples are sucrose, mannose, glycine, betaine, alanine, glutamine, glutamic acid and ammoniumsulfate. Glycerol, mannitol and sorbitol are also included in this group even though they are able to bind polar regions. Another group of additives of special interest are able to increase surface tension of the MHC molecule and simultaneously interact with charged groups in the protein. Examples are MgSO₄, NaCI, polyethylenglycol, 2-methyl-2,4-pentandiol and guanidiniumsulfate.

Correct folding of MHC I complexes is very dependent on binding of peptide in the peptide-binding cleft and the peptide binding stabilises correct conformation. Addition of molar excess of peptide will force the equilibrium against correct folded MHC-peptide complexes. Likewise is excess β2m also expected to drive the folding process in direction of correct folded MHC I complexes. Therefore peptide identical to the peptide bound in the peptide-binding cleft and β2m are included as stabilizing soluble additives.

Other additives of special interest for stabilization of MHC I molecules are BSA, fetal and bovine calf serum or individual protein components in serum with a protein stabilizing effect.

All of the above mentioned soluble additives could be added to any solution containing MHC I molecules in order to increase the stability of the molecule. That could be during the refolding process, to the soluble monomer or to a solutions containing MHC I bound to a carrier.

MHC Il molecules as used herein are defined as classical MHC Il molecule consisting of a α-chain and a β-chain combined with a peptide. It could also be a molecule only consisting of α-chain and β-chain (α/β dimer or empty MHC II), a truncated α-chain

(e.g. α1 domain alone) combined with full-length β-chain either empty or loaded with a peptide, a truncated β-chain (e.g. β1 domain alone) combined with a full-length α-chain either empty or loaded with a peptide or a truncated α-chain combined with a truncated β-chain (e.g. α1 and β1 domain) either empty or loaded with a peptide.

In contrast to MHC I molecules MHC Il molecules are not easily refolded in vitro. Only some MHC Il alleles may be produced in E. coli followed by refolding in vitro. O--

Therefore preferred expression systems for production of MHC Il molecules are eukaryotic systems where refolding after expression of protein is not necessary. Such expression systems could be stable Drosophila cell transfectants, baculovirus infected insect cells, CHO cells or other mammalian cell lines suitable for expression of proteins.

Stabilization of soluble MHC Il molecules is even more important than for MHC I molecules since both α- and β-chain are participants in formation of the peptide binding groove and tend to dissociate when not embedded in the cell membrane.

Stabilization strategies for MHC Il complexes o Generation of covalent protein -fusions.

MHC Il complexes can be stabilized by introduction of one or more linkers between the individual components of the MHC Il complex. This can be a α/β dimer with a linker between α-chain and β-chain; a α/β dimer covalently linked to the peptide via a linker to either the α-chain or β-chain; a α/β dimer, covalently linked by a linker between the α-chain and β-chain, and where the dimer is covalently linked to the peptide; a α/β dimer with a linker between α-chain and β-chain, where the dimer is combined with a peptide covalently linked to either α-chain or β-chain.

The linker can be a flexible linker, e.g. made of glycine and serine, and is typically between 5-20 residues long, but can be shorter or longer. The linker can also be more rigid with a more defined structure, e.g. made of amino acids like glutamate, alanine, lysine, and leucine.

The peptides can be linked to the NH₂- or COOH-terminus of either α-chain or β- chain. Of special interest are peptides linked to the NH₂-terminus of the β-chain via their COOH-terminus, since the linker required is shorter than if the peptide is linked to the COOH-terminus of the β-chain.

Linkage of α-chain to β-chain can be via the COOH-terminus of the β-chain to the NH₂-terminus of the α-chain or from the COOH-terminus of the α-chain to the NH₂- terminus of the β-chain. In a three-molecule fusion protein consisting of α-chain, β-chain and peptide a preferred construct is where one linker connect the COOH-terminus of the β-chain with the NH₂-terminus of the α-chain and another linker connects the COOH- terminal of the peptide with the NH₂-terminal of the β-chain. Alternatively one linker joins the COOH-terminus of the α-chain with the NH₂-terminus of the β-chain and the second linker joins the NH₂-terminus of the peptide with the COOH-terminus of the β-chain. The three peptides of the MHC complex can further be linked as described above for the three peptides of the MHC complex, including internal fusion points for the proteins.

o Non-covalent stabilization by binding ligand.

Non-covalent binding of ligands to the MHC Il complex can promote assembly of α- and β-chain by bridging the two chains, or by binding to either of the α- or β-chains, and in this way stabilize the conformation of α or β, that binds β or α, respectively, and/or that binds the peptide. Ligands here mean antibodies, peptides, aptamers or any other molecules with the ability to bind proteins.

A particular interesting example is an antibody binding the MHC complex distal to the interaction site with TCR, i.e. distal to the peptide-binding cleft. An antibody in this example can be any truncated or full length antibody of any isotype (e.g. IgG,

IgM, IgA or IgE), a bi-Fab fragment or a diabody. The antibody could be bispecific with one arm binding to the α-chain and the other arm binding to the β-chain. Alternatively the antibody could be monospecific and directed to a sequence fused to the α-chain as well as to the β-chain.

Another example of interest is an antibody binding more central in the MHC Il molecule, but still interacting with both α- and β-chain. Preferable the antibody binds a conformational epitope, thereby forcing the MHC molecule into a correct folded configuration. The antibody can be bispecific binding with one arm to the α- chain and the other arm to the β-chain. Alternatively the antibody is monospecific and binds to a surface of the complex that involves both the α- and β-chain, e.g. both the α2- and β2- domain or both the α1 - and β1 - domain.

The antibodies described above can be substituted with any other ligand that binds at the α-/β-chain interface, e.g. peptides and aptamers. The ligand can also bind the peptide, although, in this case it is important that the ligand does not interfere with the interaction of the peptide or binding cleft with the TCR.

o Non-covalent stabilization by induced multimerization.

In nature the anchoring of the α- and β-chains in the cell membrane stabilizes the MHC Il complexes considerably. As mentioned above, a similar concept for stabilization of the α/β-dimer was employed by attachment of the MHC Il chains to the Fc regions of an antibody, leading to a stable α/β-dimer, where α and β are held together by the tight interactions between two Fc domains of an antibody. Other dimerization domains can be used as well. In one other example of special interest MHC Il molecules are incorporated into artificial membrane spheres like liposomes or lipospheres. MHC Il molecules can be incorporated as monomers in the membrane or as dimers like the MHC II- antibody constructs describes above. In addition to stabilization of the MHC Il complex an increased avidity is obtained. The stabilization of the dimer will in most cases also stabilize the trimeric MHC-peptide complex.

Induced multimerization can also be achieved by biotinylation of α- as well as β- chain and the two chains brought together by binding to streptavidin. Long flexible linkers such as extended glycine-serine tracts can be used to extend both chains, and the chains can be biotinylated at the end of such extended linkers. Then streptavidin can be used as a scaffold to bring the chains together in the presence of the peptide, while the flexible linkers still allow the chains to orientate properly.

o Generation of modified proteins or protein components

Stability of MHC Il complexes can be increased by covalent modifications of the protein. One method is to increase the affinity of the peptide for the MHC complex.

This can be done by exchange of the natural amino acids with other natural or non- natural amino acids at relevant positions in the peptide or by chemical modifications of amino acids at relevant positions in the peptide. Alternatively, mutations, chemical modifications, insertion of natural or non-natural amino acids or deletions can be introduced in the peptide-binding cleft.

Mutations, chemical modifications, insertion of natural or non-natural amino acids or deletions can alternatively be introduced in α- and/or β- chain at positions outside the peptide-binding cleft. In this respect a preferred embodiment is to replace the hydrophobic transmembrane regions of α-chain and β-chain by leucine zipper dimerisation domains (e.g. Fos-Jun leucine zipper; acid-base coiled-coil structure) to promote assembly of α-chain and β-chain.

Another preferred embodiment is to introduce one or more cysteine residues by amino acid exchange at the COOH-terminal of both α-chain and β-chain, to create disulfide bridges between the two chains upon assembly of the MHC complex. Another embodiment is removal of "unwanted cysteine residues" in either of the chains by mutation, chemical modification, amino acid exchange or deletion. "Unwanted cysteine residues" is here to be understood as cysteines not involved in correct folding of the MHC ll-peptide complex. The presence of cysteines not directly involved in the formation of correctly folded MHC Il complexes can lead to formation of intra molecular disulfide bridges and incorrectly folded MHC complexes.

MHC Il complexes can also be stabilized by chemically linking together the subunits and the peptide. That can be a linker between peptide and α-chain, between peptide and β-chain, between α-chain and β-chain, and combination thereof.

Such linkages can be introduced prior to folding by linking two of the complex constituents together, then folding this covalent hetero-dimer in the presence of the third constituent. An advantage of this method is that it only requires complex formation between two, rather than three species.

Another possibility is to allow all three constituents to fold, and then to introduce covalent cross-links on the folded MHC-complex, stabilizing the structure. An advantage of this method is that the two chains and the peptide will be correctly positioned relatively to each other when the cross linkages are introduced.

o Stabilization with soluble additives.

Salts, detergents, organic solvent, polymers and any other soluble additives can be added to increase the stability of MHC complexes. Of special interest are additives that increase surface tension of the MHC complex. Examples are sucrose, mannose, glycine, betaine, alanine, glutamine, glutamic acid and ammonium sulfate. Glycerol, mannitol and sorbitol are also included in this group even though they are able to bind polar regions.

Another group of additives of special interest increases surface tension of the MHC complex and simultaneously can interact with charged groups in the protein. Examples are MgSO₄, NaCI, polyethylenglycol, 2-methyl-2,4-pentanediol and guanidiniumsulphate.

Correct formation of MHC complexes is dependent on binding of peptide in the peptide-binding cleft; the bound peptide appears to stabilize the complex in its correct conformation. Addition of molar excess of peptide will force the equilibrium towards correctly folded MHC-peptide complexes. Likewise, excess β2m is also expected to drive the folding process in direction of correctly folded MHC complexes. Therefore peptide identical to the peptide bound in the peptide-binding cleft and β2m can be included as stabilizing soluble additives.

Other additives of special interest for stabilization of MHC complexes are BSA, fetal and bovine calf serum, and other protein components in serum with a protein stabilizing effect.

All of the above mentioned soluble additives could be added to any solution containing MHC complexes in order to increase the stability of the molecule. This can be during the refolding process, to the formed MHC complex or to a solution of MHC multimers comprising several MHC complexesThat could be to the soluble monomer, to a solution containing MHC Il bound to a carrier or to solutions used during analysis of MHC Il specific T cells with MHC Il multimers.

Other additives of special interest for stabilization of MHC Il molecules are BSA, fetal and bovine calf serum or individual protein components in serum with a protein stabilizing effect.

All of the above mentioned soluble additives could be added to any solution containing MHC Il molecules in order to increase the stability of the molecule. That could be to the soluble monomer, to a solution containing MHC Il bound to a carrier or to solutions used during analysis of MHC Il specific T cells with MHC Il multimers.

Chemically modified MHC I and Il complexes There are a number of amino acids that are particularly reactive towards chemical cross linkers. In the following, chemical reactions are described that are particularly preferable for the cross-linking or modification of MHC I or MHC Il complexes. The amino group at the N-terminal of both chains and of the peptide, as well as amino groups of lysine side chains, are nucleophilic and can be used in a number of chemical reactions, including nucleophilic substitution by activation of electrophiles (e.g. acylation such as amide formation, pyrazolone formation, isoxazolone formation; alkylation; vinylation; disulfide formation), addition to carbon-hetero multiple bonds (e.g. alkene formation by reaction of phosphonates with aldehydes or ketones; arylation; alkylation of arenes/hetarenes by reaction with alkyl boronates or enolethers), nucleophilic substitution using activation of nucleophiles (e.g. condensations; alkylation of aliphatic halides or tosylates with enolethers or enamines), and cycloadditions. Example reagents that can be used in a reaction with the amino groups are activated carboxylic acids such as NHS-ester, tetra and pentafluoro phenolic esters, anhydrides, acid chlorides and fluorides, to form stable amide bonds. Likewise, sulphonyl chlorides can react with these amino groups to form stable sulphone-amides. Iso-Cyanates can also react with amino groups to form stable ureas, and isothiocyanates can be used to introduce thiourea linkages.

Aldehydes, such as formaldehyde and glutardialdehyde will react with amino groups to form shiff's bases, than can be further reduced to secondary amines. The guanidino group on the side chain of arginine will undergo similar reactions with the same type of reagents.

Another very useful amino acid is cysteine. The thiol on the side chain is readily alkylated by maleimides, vinyl sulphones and halides to form stable thioethers, and reaction with other thiols will give rise to disulphides. o

Carboxylic acids at the C-terminal of both chains and peptide, as well as on the side chains of glutamic and aspartic acid, can also be used to introduce cross-links. They will require activation with reagents such as carbodiimides, and can then react with amino groups to give stable amides.

Thus, a large number of chemistries can be employed to form covalent cross-links. The crucial point is that the chemical reagents are bi-functional, being capable of reacting with two amino acid residues.

They can be either homo bi-functional, possessing two identical reactive moieties, such as glutardialdehyde or can be hetero bi-functional with two different reactive moieties, such as GMBS (MaleimidoButyryloxy-Succinimide ester).

Alternatively, two or more reagents can be used; i.e. GMBS can be used to introduce maleimides on the α-chain, and iminothiolane can be used to introduce thiols on the β-chain; the malemide and thiol can then form a thioether link between the two chains.

For the present invention some types of cross-links are particularly useful. The folded MHC-complex can be reacted with dextrans possessing a large number (up to many hundreds) of vinyl sulphones. These can react with lysine residues on both the α and β chains as well as with lysine residues on the peptide protruding from the binding site, effectively cross linking the entire MHC-complex. Such cross linking is indeed a favored reaction because as the first lysine residue reacts with the dextran, the MHC-complex becomes anchored to the dextran favoring further reactions between the MHC complex and the dextran multimerization domain.

Another great advantage of this dextran chemistry is that it can be combined with fluorochrome labelling; i.e. the dextran is reacted both with one or several MHC- complexes and one or more fluorescent protein such as APC.

Another valuable approach is to combine the molecular biological tools described above with chemical cross linkers. As an example, one or more lysine residues can be inserted into the α-chain, juxtaposed with glutamic acids in the β-chain, where after the introduced amino groups and carboxylic acids are reacted by addition of carbodiimide. Such reactions are usually not very effective in water, unless as in this case, the groups are well positioned towards reaction. This implies that one avoids excessive reactions that could otherwise end up denaturing or changing the conformation of the MHC-complex.

Likewise a dextran multimerization domain can be cross-linked with appropriately modified MHC-complexes; i.e. one or both chains of the MHC complex can be enriched with lysine residues, increasing reactivity towards the vinylsulphone dextran. The lysine's can be inserted at positions opposite the peptide binding cleft, orienting the MHC-complexes favorably for T-cell recognition.

Another valuable chemical tool is to use extended and flexible cross-linkers. An extended linker will allow the two chains to interact with little or no strain resulting from the linker that connects them, while keeping the chains in the vicinity of each other should the complex dissociate. An excess of peptide should further favor reformation of dissociated MHC-complex.

Other TCR binding molecules

MHC I and MHC Il complexes bind to TCRs. However, other molecules also bind TCR. Some TCR-binding molecules are described in the following. MHC I and MHC Il complexes binding to TCRs may be substituted with other molecules capable of binding TCR or molecules that have homology to the classical MHC molecules and therefore potentially could be TCR binding molecules. These other TCR binding or MHC like molecules include:

Non-classical MHC complexes and other MHC-like molecules: Non-classical MHC complexes include protein products of MHC Ib and MHC Nb genes. MHC Ib genes encode β2m-associated cell-surface molecules but show little polymorphism in contrast to classical MHC class I genes. Protein products of MHC class Ib genes include HLA-E, HLA-G, HLA-F, HLA-H, MIC A, MIC B, ULBP-1 , ULBP- 2, ULBP-3 in humans and H2-M, H2-Q, H2-T and Rae1 in mice.

Non-classical MHC Il molecules (protein products of MHC Nb genes) include HLA-DM, HLA-DO in humans and H2-DM and H2-DO in mice that are involved in regulation of peptide loading into MHC Il molecules. Another MHC-like molecule of special interest is the MHC l-like molecule CD1 . CD1 is similar to MHC I molecules in its organization of subunits and association with β2m but presents glycolipids and lipids instead of peptides.

Artificial molecules capable of binding specific TCRs

Of special interest are antibodies that bind TCRs. Antibodies herein include full length antibodies of isotype IgG, IgM, IgE, IgA and truncated versions of these, antibody fragments like Fab fragments and scFv. Antibodies also include antibodies of antibody fragments displayed on various supramolecular structures or solid supports, including filamentous phages, yeast, mammalian cells, fungi, artificial cells or micelles, and beads with various surface chemistries.

Peptide binding TCR Another embodiment of special interest is peptides that bind TCRs. Peptides herein include peptides composed of natural, non-natural and/or chemically modified amino acids with a length of 8-20 amino acid. The peptides could also be longer than 20 amino acids or shorter than 8 amino acids. The peptides can or can not have a defined tertiary structure.

Aptamers

Aptamers are another preferred group of TCR ligands. Aptamers are herein understood as natural nucleic acids (e.g. RNA and DNA) or unnatural nucleic acids (e.g. PNA, LNA, morpholinos) capable of binding TCR. The aptamer molecules consist of natural or modified nucleotides in various lengths.

Other TCR-binding molecules can be ankyrin repeat proteins or other repeat proteins, Avimers, or small chemical molecules, as long as they are capable of binding TCR with a dissociation constant smaller than 10^'3 M..

Verification of correctly folded MHC-peptide complexes

Quantitative ELISA and other techniques to quantify correctly folded MHC complexes

When producing MHC multimers, it is desirable to determine the degree of correctly folded MHC.

The fraction or amount of functional and/or correctly folded MHC can be tested in a number of different ways, including: • Measurement of correctly folded MHC in a quantitative ELISA, e.g. where the MHC bind to immobilized molecules recognizing the correctly folded complex.

• Measurement of functional MHC in an assay where the total protein concentration is measured before functional MHC is captured, by binding to e.g. immobilized TCR, and the excess, non-bound protein are measured. If the dissociation constant for the interaction is known, the amount of total and the amount of non-bound protein can be determined. From these numbers, the fraction of functional MHC complex can be determined.

• Measurement of functional MHC complex by a non-denaturing gel-shift assay, where functional MHC complexes bind to TCR (or another molecule that recognize correctly folded MHC complex), and thereby shifts the TCR to another position in the gel.

Multimerization domain A number of MHC complexes associate with a multimerization domain to form a MHC multimer. The size of the multimerization domain spans a wide range, from multimerisation domains based on small organic molecule scaffolds to large multimers based on a cellular structure or solid support. The multimerization domain may thus be based on different types of carriers or scaffolds, and likewise, the attachment of MHC complexes to the multimerization domain may involve covalent or non-covalent linkers. Characteristics of different kinds of multimerization domains are described below.

Molecular weight of multimerization domain.

• In one embodiment the multimerization domain(s) in the present invention is preferably less than 1 ,000 Da (small molecule scaffold). Examples include short peptides (e.g. comprising 10 amino acids), and various small molecule scaffolds (e.g. aromatic ring structures).

• In another embodiment the multimerization domain(s) is preferably between 1 ,000 Da and 10,000 Da (small molecule scaffold, small peptides, small polymers). Examples include polycyclic structures of both aliphatic and aromatic compounds, peptides comprising e.g. 10-100 amino acids, and other polymers such as dextran, polyethylenglycol, and polyureas.

• In another embodiment the multimerization domain(s) is between 10,000 Da and 100,000 Da (Small molecule scaffold, polymers e.g. dextran, streptavidin, IgG, pentamer structure). Examples include proteins and large polypeptides, small molecule scaffolds such as steroids, dextran, dimeric streptavidin, and multi-subunit proteins such as used in Pentamers.

• In another embodiment the multimerization domain(s) is preferably between 100,000 Da and 1 ,000,000 Da (Small molecule scaffold, polymers e.g. dextran, streptavidin, IgG, pentamer structure). Typical examples include larger polymers such as dextran (used in e.g. Dextramers), and streptavidin tetramers.

• In another embodiment the multimerization domain(s) is preferably larger than 1 ,000,000 Da (Small molecule scaffold, polymers e.g. dextran, streptavidin, IgG, pentamer structure, cells, liposomes, artificial lipid bilayers, polystyrene beads and other beads. Most examples of this size involve cells or cell-based structures such as micelles and liposomes, as well as beads and other solid supports.

As mentioned elsewhere herein multimerisation domains can comprise carrier molecules, scaffolds or combinations of the two.

Type of multimerization domain.

In principle any kind of carrier or scaffold can be used as multimerization domain, including any kind of cell, polymer, protein or other molecular structure, or particles and solid supports. Below different types and specific examples of multimerization domains are listed.

• Cell. Cells can be used as carriers. Cells can be either alive and mitotic active, alive and mitotic inactive as a result of irradiation or chemically treatment, or the cells may be dead. The MHC expression may be natural (i.e. not stimulated) or may be induced/stimulated by e.g. lnf-γ. Of special interest are natural antigen presenting cells (APCs) such as dendritic cells, macrophages, Kupfer cells, Langerhans cells, B-cells and any MHC expressing cell either naturally expressing, being transfected or being a hybridoma. • Cell-like structures. Cell-like carriers include membrane-based structures carrying MHC-peptide complexes in their membranes such as micelles, liposomes, and other structures of membranes, and phages such as filamentous phages.

• Solid support. Solid support includes beads, particulate matters and other surfaces. A preferred embodiment include beads (magnetic or non-magnetic beads) that carry electrophilic groups e.g. divinyl sulfone activated polysaccharide, polystyrene beads that have been functionalized with tosyl- activated esters, magnetic polystyrene beads functionalized with tosyl-activated esters), and where MHC complexes may be covalently immobilized to these by reaction of nucleophiles comprised within the MHC complex with the electrophiles of the beads. Beads may be made of sepharose, sephacryl, polystyrene, agarose, polysaccharide, polycarbamate or any other kind of beads that can be suspended in aqueous buffer. Another embodiment includes surfaces, i.e. solid supports and particles carrying immobilized MHC complexes on the surface. Of special interest are wells of a microtiter plate or other plate formats, reagent tubes, glass slides or other supports for use in microarray analysis, tubings or channels of micro fluidic chambers or devices, Biacore chips and beads Molecule. Multimerization domains may also be molecules or complexes of molecules held together by non-covalent bonds. The molecules constituting the multimerization domain can be small organic molecules or large polymers, and may be flexible linear molecules or rigid, globular structures such as e.g. proteins. Different kinds of molecules used in multimerization domains are described below. o Small organic molecules. Small organic molecules here includes steroids, peptides, linear or cyclic structures, and aromatic or aliphatic structures, and many others. The prototypical small organic scaffold is a functionalized benzene ring, i.e. a benzene ring functionalized with a number of reactive groups such as amines, to which a number of MHC molecules may be covalently linked. However, the types of reactive groups constituting the linker connecting the MHC complex and the multimerization domain, as well as the type of scaffold structure, can be chosen from a long list of chemical structures. A non-comprehensive list of scaffold structures are listed below. Typical scaffolds include aromatic structures, benzodiazepines, hydantoins, piperazines, indoles, furans, thiazoles, steroids, diketopiperazines, morpholines, tropanes, coumarines, qinolines, pyrroles, oxazoles, amino acid precursors, cyclic or aromatic ring structures, and many others. Typical carriers include linear and branched polymers such as peptides, polysaccharides, nucleic acids, and many others. Multimerization domains based on small organic or polymer molecules thus include a wealth of different structures, including small compact molecules, linear structures, polymers, polypeptides, polyureas, polycarbamates, cyclic structures, natural compound derivatives, alpha-, beta-, gamma-, and omega-peptides, mono-, di- and tri-substituted peptides, L- and D-form peptides, cyclohexane- and cyclopentane-backbone modified beta- peptides, vinylogous polypeptides, glycopolypeptides, polyamides, vinylogous sulfonamide peptide, Polysulfonamide-conjugated peptide (i.e., having prosthetic groups), Polyesters, Polysaccharides such as dextran and aminodextran, polycarbamates, polycarbonates, polyureas, poly- peptidylphosphonates, Azatides, peptoids (oligo N-substituted glycines), Polyethers, ethoxyformacetal oligomers, poly-thioethers, polyethylene, glycols (PEG), polyethylenes, polydisulfides, polyarylene sulfides,

Polynucleotides, PNAs, LNAs, Morpholinos, oligo pyrrolinone, polyoximes, Polyimines, Polyethyleneimine, Polyacetates, Polystyrenes, Polyacetylene, Polyvinyl, Lipids, Phospholipids, Glycolipids, polycycles, (aliphatic), polycycles (aromatic), polyheterocycles, Proteoglycan, Polysiloxanes, Polyisocyanides, Polyisocyanates, polymethacrylates,

Monofunctional, Difunctional, Trifunctional and Oligofunctional open-chain hydrocarbons, Monofunctional, Difunctional, Trifunctional and Oligofunctional Nonaromat Carbocycles, Monocyclic, Bicyclic, Tricyclic and Polycyclic Hydrocarbons, Bridged Polycyclic Hydrocarbones, Monofunctional, Difunctional, Trifunctional and Oligofunctional

Nonaromatic, Heterocycles, Monocyclic, Bicyclic, Tricyclic and Polycyclic Heterocycles, bridged Polycyclic Heterocycles, Monofunctional, Difunctional, Trifunctional and Oligofunctional Aromatic Carbocycles, Monocyclic, Bicyclic, Tricyclic and Polycyclic Aromatic Carbocycles, Monofunctional, Difunctional, Trifunctional and Oligofunctional Aromatic

Hetero-cycles. Monocyclic, Bicyclic, Tricyclic and Polycyclic Heterocycles. Chelates, fullerenes, and any combination of the above and many others. Biological polymers. Biological molecules here include peptides, proteins (including antibodies, coiled-coil helices, streptavidin and many others), nucleic acids such as DNA and RNA, and polysaccharides such as dextran. The biological polymers may be reacted with MHC complexes (e.g. a number of MHC complexes chemically coupled to e.g. the amino groups of a protein), or may be linked through e.g. DNA duplex formation between a carrier DNA molecule and a number of DNA oligonucleotides each coupled to a MHC complex. Another type of multimerization domain based on a biological polymer is the streptavidin-based tetramer, where a streptavidin binds up to four biotinylated MHC complexes, as described above (see Background of the invention). o Self-assembling multimeric structures. Several examples of commercial

MHC multimers exist where the multimer is formed through self- assembling. Thus, the Pentamers are formed through formation of a coiled-coil structure that holds together 5 MHC complexes in an apparently planar structure. In a similar way, the Streptamers are based on the Streptactin protein which oligomerizes to form a MHC multimer comprising several MHC complexes (see Background of the invention).

In the following, alternative ways to make MHC multimers based on a molecule multimerization domain are described. They involve one or more of the abovementoned types of multimerization domains.

MHC dextramers can be made by coupling MHC complexes to dextran via a streptavidin-biotin interaction. In principle, biotin-streptavdin can be replaced by any dimerization domain, where one half of the dimerization domain is coupled to the MHC- peptide complex and the other half is coupled to dextran. For example, an acidic helix (one half of a coiled-coil dimer) is coupled or fused to MHC, and a basic helix (other half of a coiled-coil dimmer) is coupled to dextran. Mixing the two results in MHC binding to dextran by forming the acid/base coiled-coil structure.

Antibodies can be used as scaffolds by using their capacity to bind to a carefully selected antigen found naturally or added as a tag to a part of the MHC molecule not involved in peptide binding. For example, IgG and IgE will be able to bind two MHC molecules, IgM having a pentameric structure will be able to bind 10 MHC molecules. The antibodies can be full-length or truncated; a standard antibody-fragment includes the Fab2 fragment. Peptides involved in coiled-coil structures can act as scaffold by making stable dimeric, trimeric, tetrameric and pentameric interactions. Examples hereof are the Fos-Jun heterodimeric coiled coil, the E. coli homo-trimeric coiled-coil domain Lpp-56, the engineered Trp-zipper protein forming a discrete, stable, α-helical pentamer in water at physiological pH.

Further examples of suitable scaffolds, carriers and linkers are streptavidin (SA) and avidin and derivatives thereof, biotin, immunoglobulins, antibodies (monoclonal, polyclonal, and recombinant), antibody fragments and derivatives thereof, leucine zipper domain of AP- 1 (jun and fos), hexa-his (metal chelate moiety), hexa-hat GST (glutathione S-tranf erase), glutathione, Calmodulin-binding peptide (CBP), Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, E2Tag, HA Epitope Tag, Myc Epitope, FLAG Epitope, AU1 and AU5 Epitopes, GIu-GIu Epitope, KT3 Epitope, IRS Epitope, Btag Epitope, Protein Kinase-C Epitope, VSV Epitope, lectins that mediate binding to a diversity of compounds, including carbohydrates, lipids and proteins, e.g. Con A {Canavalia ensiformis) or WGA (wheat germ agglutinin) and tetranectin or Protein A or G (antibody affinity). Combinations of such binding entities are also comprised. Non- limiting examples are streptavidin-biotin and jun-fos. In particular, when the MHC molecule is tagged, the binding entity may be an "anti-tag". By "anti-tag" is meant an antibody binding to the tag, or any other molecule capable of binding to such tag.

MHC complexes can be multimerized by other means than coupling or binding to a multimerization domain. Thus, the multimerization domain may be formed during the multimerization of MHCs. One such method is to extend the bound antigenic peptide with dimerization domains. One end of the antigenic peptide is extended with dimerization domain A (e.g. acidic helix, half of a coiled-coil dimer) and the other end is extended with dimerization domain B (e.g. basic helix, other half of a coiled-coil dimer). When MHC complexes are loaded/mixed with these extended peptides the following multimer structure will be formed: A-MHC-BA-MHC-BA-MHC-B etc. The antigenic peptides in the mixture can either be identical or a mixture of peptides with comparable extended dimerization domains. Alternatively both ends of a peptide are extended with the same dimerization domain A and another peptide (same amino acid sequence or a different amino acid sequence) is extended with dimerization domain B. When MHC o

and peptides are mixed the following structures are formed: A-MHC-AB-MHC-BA- MHC-AB-MHC-B etc. Multimerization of MHC complexes by extension of peptides are restricted to MHC Il molecules since the peptide binding groove of MHC I molecules is typically closed in both ends thereby limiting the size of peptide that can be embedded in the groove, and therefore preventing the peptide from extending out of the groove.

Another multimerization approach applicable to both MHC I and MHC Il complexes is based on extension of N- and C-terminal of the MHC complex. For example the N- terminal of the MHC complex is extended with dimerization domain A and the C- terminal is extended with dimerization domain B. When MHC complexes are incubated together they pair with each other and form multimers like: A-MHC-BA-MHC-BA-MHC- BA-MHC-B etc. Alternatively the N-terminal and the C-terminal of a MHC complex are both extended with dimerization domain A and the N-terminal and C-terminal of another preparation of MHC complex (either the same or a different MHC) are extended with dimerization domain B. When these two types of MHC complexes are incubated together multimers will be formed: A-MHC-AB-MHC-BA-MHC-AB-MHC-B etc.

In all the above-described examples the extension can be either chemically coupled to the peptide/MHC complex or introduced as extension by gene fusion.

Dimerization domain AB can be any molecule pair able to bind to each other, such as acid/base coiled-coil helices, antibody-antigen, DNA-DNA, PNA-PNA, DNA-PNA, DNA- RNA, LNA-DNA, leucine zipper e.g. Fos/Jun, streptavidin-biotin and other molecule pairs as described elsewhere herein.

Linker molecules.

A number of MHC complexes associate with a multimerization domain to form a MHC multimer. The attachment of MHC complexes to the multimerization domain may involve covalent or non-covalent linkers, and may involve small reactive groups as well as large protein-protein interactions.

The coupling of multimerization domains and MHC complexes involve the association of an entity X (attached to or part of the multimerization domain) and an entity Y (attached to or part of the MHC complex). Thus, the linker that connects the multimerization domain and the MHC complex comprises an XY portion. Covalent linker. The XY linkage can be covalent, in which case X and Y are reactive groups. In this case, X can be a nucleophilic group (such as -NH₂, - OH, -SH, -NH-NH₂), and Y an electrophilic group (such as CHO, COOH, CO) that react to form a covalent bond XY; or Y can be a nucleophilic group and X an electrophilic group that react to form a covalent bond XY. Other possibilities exist, e.g either of the reactive groups can be a radical, capable of reacting with the other reactive group. A number of reactive groups X and Y, and the bonds that are formed upon reaction of X and Y, are shown in figure 5. X and Y can be reactive groups naturally comprised within the multimerization domain and/or the MHC complex, or they can be artificially added reactive groups. Thus, linkers containing reactive groups can be linked to either of the multimerization domain and MHC complex; subsequently the introduced reactive group(s) can be used to covalently link the multimerization domain and MHC complex. Example natural reactive groups of MHC complexes include amino acid side chains comprising -NH₂, -OH, -SH, and -NH-. Example natural reactive groups of multimerization domains include hydroxyls of polysaccharides such as dextrans, but also include amino acid side chains comprising -NH₂, -OH, -SH, and -NH- of polypeptides, when the polypeptide is used as a multimerization domain. In some MHC multimers, one of the polypeptides of the MHC complex

(i.e. the β2M, heavy chain or the antigenic peptide) is linked by a protein fusion to the multimerization domain. Thus, during the translation of the fusion protein, an acyl group (reactive group X or Y) and an amino group (reactive group Y or X) react to form an amide bond. Example MHC multimers where the bond between the multimerization domain and the MHC complex is covalent and results from reaction between natural reactive groups, include MHC-pentamers (described in US patent 2004209295) and MHC-dimers, where the linkage between multimerization domain and MHC complex is in both cases generated during the translation of the fusion protein. Example artificial reactive groups include reactive groups that are attached to the multimerization domain or MHC complex, through association of a linker molecule comprising the reactive group. The activation of dextran by reaction of the dextran hydroxyls with divinyl sulfone, introduces a reactive vinyl group that can react with e.g. amines of the MHC complex, to form an amine that now links the multimerization domain (the dextran polymer) and the MHC complex. An o

alternative activation of the dextran multimerization domain involves a multistep reaction that results in the decoration of the dextran with maleimide groups, as described in the patent Siiman et al. US 6,387,622. In this approach, the amino groups of MHC complexes are converted to -SH groups, capable of reacting with the maleimide groups of the activated dextran. Thus, in the latter example, both the reactive group of the multimerization domain (the maleimide) and the reactive group of the MHC complex (the thiol) are artificially introduced.

Sometimes activating reagents are used in order to make the reactive groups more reactive. For example, acids such as glutamate or aspartate can be converted to activated esters by addition of e.g. carbodiimid and NHS or nitrophenol, or by converting the acid moiety to a tosyl-activated ester. The activated ester reacts efficiently with a nucleophile such as -NH₂, -SH, -OH, etc.

For the purpose of this invention, the multimerization domains (including small organic scaffold molecules, proteins, protein complexes, polymers, beads, liposomes, micelles, cells) that form a covalent bond with the MHC complexes can be divided into separate groups, depending on the nature of the reactive group that the multimerization domain contains. One group comprise multimerization domains that carry nucleophilic groups (e.g. -NH₂, -OH, -SH, -

CN, -NH-NH₂), exemplified by polysaccharides, polypeptides containing e.g. lysine, serine, and cysteine; another group of multimerization domains carry electrophilic groups (e.g. -COOH, -CHO, -CO, NHS-ester, tosyl-activated ester, and other activated esters, acid-anhydrides), exemplified by polypeptides containing e.g. glutamate and aspartate, or vinyl sulfone activated dextran; yet another group of multimerization domains carry radicals or conjugated double bonds.

The multimerization domains appropriate for this invention thus include those that contain any of the reactive groups shown in Figure 5 or that can react with other reactive groups to form the bonds shown in Figure 5.

Likewise, MHC complexes can be divided into separate groups, depending on the nature of the reactive group comprised within the MHC complex. One group comprise MHCs that carry nucleophilic groups (e.g. -NH₂, -OH, -SH, -CN, -NH- NH₂), e.g. lysine, serine, and cysteine; another group of MHCs carry electrophilic groups (e.g. -COOH, -CHO, -CO, NHS-ester, tosyl-activated ester, and other activated esters, acid-anhydrides), exemplified by e.g. glutamate and aspartate; yet another group of MHCs carry radicals or conjugated double bonds.

The reactive groups of the MHC complex are either carried by the amino acids of the MHC-peptide complex (and may be comprised by any of the peptides of the MHC-peptide complex, including the antigenic peptide), or alternatively, the reactive group of the MHC complex has been introduced by covalent or non- covalent attachment of a molecule containing the appropriate reactive group.

Preferred reactive groups in this regard include -CSO₂OH, phenylchloride, -SH, -SS, aldehydes, hydroxyls, isocyanate, thiols, amines, esters, thioesters, carboxylic acids, triple bonds, double bonds, ethers, acid chlorides, phosphates, imidazoles, halogenated aromatic rings, any precursors thereof, or any protected reactive groups, and many others. Example pairs of reactive groups, and the resulting bonds formed, are shown in figure 5.

Reactions that may be employed include acylation (formation of amide, pyrazolone, isoxazolone, pyrimidine, comarine, quinolinon, phthalhydrazide, diketopiperazine, benzodiazepinone, and hydantoin), alkylation, vinylation, disulfide formation, Wittig reaction, Horner-Wittig-Emmans reaction, arylation (formation of biaryl or vinylarene), condensation reactions, cycloadditions ((2+4), (3+2)), addition to carbon-carbon multiplebonds, cycloaddition to multiple bonds, addition to carbon-hetero multiple bonds, nucleophilic aromatic substitution, transition metal catalyzed reactions, and may involve formation of ethers, thioethers, secondary amines, tertiary amines, beta-hydroxy ethers, beta-hydroxy thioethers, beta-hydroxy amines, beta-amino ethers, amides, thioamides, oximes, sulfonamides, di- and tri-functional compounds, substituted aromatic compounds, vinyl substituted aromatic compounds, alkyn substituted aromatic compounds, biaryl compounds, hydrazines, hydroxylamine ethers, substituted cycloalkenes, substituted cyclodienes, substituted 1 , 2, 3 triazoles, substituted cycloalkenes, beta-hydroxy ketones, beta-hydroxy aldehydes, vinyl ketones, vinyl aldehydes, substituted alkenes, substituted alkenes, substituted amines, and many others. O-.

MHC dextramers can be made by covalent coupling of MHC complexes to the dextran backbone, e.g. by chemical coupling of MHC complexes to dextran backbones. The MHC complexes can be coupled through either heavy chain or β2-microglobulin if the MHC complexes are MHC I or through α-chain or β- chain if the MHC complexes are MHC II. MHC complexes can be coupled as folded complexes comprising heavy chain/beta2microglobulin or α-chain/β- chain or either combination together with peptide in the peptide-binding cleft. Alternatively either of the protein chains can be coupled to dextran and then folded in vitro together with the other chain of the MHC complex not coupled to dextran and together with peptide. Direct coupling of MHC complexes to dextran multimerization domain can be via an amino group or via a sulphide group. Either group can be a natural component of the MHC complex or attached to the MHC complex chemically. Alternatively, a cysteine may be introduced into the genes of either chain of the MHC complex.

Another way to covalently link MHC complexes to dextran multimerization domains is to use the antigenic peptide as a linker between MHC and dextran. Linker containing antigenic peptide at one end is coupled to dextran. Antigenic peptide here means a peptide able to bind MHC complexes in the peptide- binding cleft. As an example, 10 or more antigenic peptides may be coupled to one dextran molecule. When MHC complexes are added to such peptide- dextran construct the MHC complexes will bind the antigenic peptides and thereby MHC-peptide complexes are displayed around the dextran multimerization domain. The antigenic peptides can be identical or different from each other. Similarly MHC complexes can be either identical or different from each other as long as they are capable of binding one or more of the peptides on the dextran multimerization domain.

Non-covalent linker. The linker that connects the multimerization domain and the MHC complex comprises an XY portion. Above different kinds of covalent linkages XY were described. However, the XY linkage can also be non- covalent. Non-covalent XY linkages can comprise natural dimerization pairs such as antigen-antibody pairs, DNA-DNA interactions, or can include natural O

interactions between small molecules and proteins, e.g. between biotin and streptavidin. Artificial XY examples include XY pairs such as His₆ tag (X) interacting with Ni-NTA (Y) and PNA-PNA interations.

Protein-protein interactions. The non-covalent linker may comprise a complex of two or more polypeptides or proteins, held together by non-covalent interactions. Example polypeptides and proteins belonging to this group include Fos/Jun, Acid/Base coiled coil structure, antibody/antigen (where the antigen is a peptide), and many others.

A preferred embodiment involving non-covalent interactions between polypeptides and/or proteins are represented by the Pentamer structure described in US patent 2004209295.

Another preferred embodiment involves the use of antibodies, with affinity for the surface of MHC opposite to the peptide-binding groove. Thus, an anti-MHC antibody, with its two binding site, will bind two MHC complexes and in this way generate a bivalent MHC multimer. In addition, the antibody can stabilize the MHC complex through the binding interactions. This is particularly relevant for MHC class Il complexes, as these are less stable than class I MHC complexes.

Polynucleotide-polynucleotide interactions. The non-covalent linker may comprise nucleotides that interact non-covalently. Example interactions include PNA/PNA, DNA/DNA, RNA/RNA, LNA/DNA, and any other nucleic acid duplex structure, and any combination of such natural and unnatural polynucleotides such as DNA/PNA, RNA/DNA, and PNA/LNA.

Protein-small molecule interactions. The non-covalent linker may comprise a macromolecule (e.g. protein, polynucleotide) and a small molecule ligand of the macromolecule. The interaction may be natural (i.e., found in Nature, such as the Streptavidin/biotin interaction) or non-natural (e.g. His-tag peptide/Ni-NTA interaction). Example interactions include Streptavidin/biotin and anti-biotin antibody/biotin. Combinations - non-covalent linker molecules. Other combinations of proteins, polynucleotides, small organic molecules, and other molecules, may be used to link the MHC to the multimerization domain. These other combinations include protein-DNA interactions (e.g. DNA binding protein such as the gene regulatory protein CRP interacting with its DNA recognition sequence), RNA aptamer- protein interactions (e.g. RNA aptamer specific for growth hormone interacting with growth hormone)

Synthetic molecule-synthetic molecule interaction. The non-covalent linker may comprise a complex of two or more organic molecules, held together by non- covalent interactions. Example interactions are two chelate molecules binding to the same metal ion (e.g. E DTA- N i⁺⁺- NTA), or a short polyhistidine peptide (e.g. HiS₆) bound to NTA-Ni⁺⁺.

In another preferred embodiment the multimerization domain is a bead. The bead is covalently or non-covalently coated with MHC multimers or single MHC complexes, through non-cleavable or cleavable linkers. As an example, the bead can be coated with streptavidin monomers, which in turn are associated with biotinylated MHC complexes; or the bead can be coated with streptavidin tetramers, each of which are associated with 0, 1 , 2, 3, or 4 biotinylated MHC complexes; or the bead can be coated with MHC-dextramers where e.g. the reactive groups of the MHC-dextramer (e.g. the divinyl sulfone-activated dextran backbone) has reacted with nucleophilic groups on the bead, to form a covalent linkage between the dextran of the dextramer and the beads.

In another preferred embodiment, the MHC multimers described above (e.g. where the multimerization domain is a bead) further contains a flexible or rigid, and water soluble, linker that allows for the immobilized MHC complexes to interact efficiently with cells, such as T-cells with affinity for the MHC complexes. In yet another embodiment, the linker is cleavable, allowing for release of the MHC complexes from the bead. If T-cells have been immobilized, by binding to the MHC complexes, the T-cells can very gently be released by cleavage of this cleavable linker. Appropriate cleavable linkers are shown in Figure 6. Most preferably, the linker is cleaved at physiological conditions, allowing for the integrity of the isolated cells. Further examples of linker molecules that may be employed in the present invention include Calmodulin-binding peptide (CBP), 6xHIS, Protein A, Protein G, biotin, Avidine, Streptavidine, Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, GST tagged proteins, E2Tag, HA Epitope Tag, Myc Epitope, FLAG Epitope, AU1 and AU5

Epitopes, GIu-GIu Epitope, KT3 Epitope, IRS Epitope, Btag Epitope, Protein Kinase-C Epitope, VSV Epitope.

The list of dimerization- and multimerization domains, described elsewhere in this document, define alternative non-covalent linkers between the multimerization domain and the MHC complex.

The abovementioned dimerization- and multimerization domains represent specific binding interactions. Another type of non-covalent interactions involves the non-specific adsorption of e.g. proteins onto surfaces. As an example, the non-covalent adsorption of proteins onto glass beads represents this class of XY interactions. Likewise, the interaction of MHC complexes (comprising full-length polypeptide chains, including the transmembrane portion) with the cell membrane of for example dendritic cells is an example of a non-covalent, primarily non-specific XY interaction.

In some of the abovementioned embodiments, several multimerization domains (e.g. streptavidin tetramers bound to biotinylated MHC complexes) are linked to another multimerization domain (e.g. the bead). For the purpose of this invention we shall call both the smaller and the bigger multimerization domain, as well as the combined multimerization domain, for multimerization domain

Additional features of product

Additional components may be coupled to carrier or added as individual components not coupled to carrier

Attachment of biologically active molecules to MHC multimers

Engagement of MHC complex to the specific T cell receptor leads to a signaling cascade in the T cell. However, T-cells normally respond to a single signal stimulus by going into apoptosis. T cells needs a second signal in order to become activated and o

start development into a specific activation state e.g. become an active cytotoxic T cell, helper T cell or regulatory T cell.

It is to be understood that the MHC multimer of the invention may further comprise one or more additional substituents. The definition of the terms "one or more", "a plurality", "a", "an", and "the" also apply here. Such biologically active molecules may be attached to the construct in order to affect the characteristics of the constructs, e.g. with respect to binding properties, effects, MHC molecule specificities, solubility, stability, or detectability. For instance, spacing could be provided between the MHC complexes, one or both chromophores of a Fluorescence Resonance Energy Transfer (FRET) donor/acceptor pair could be inserted, functional groups could be attached, or groups having a biological activity could be attached.

MHC multimers can be covalently or non-covalently associated with various molecules: having adjuvant effects; being immune targets e.g. antigens; having biological activity e.g. enzymes, regulators of receptor activity, receptor ligands, immune potentiators, drugs, toxins, co-receptors, proteins and peptides in general; sugar moieties; lipid groups; nucleic acids including siRNA; nano particles; small molecules. In the following these molecules are collectively called biologically active molecules. Such molecules can be attached to the MHC multimer using the same principles as those described for attachment of MHC complexes to multimerisation domains as described elsewhere herein. In brief, attachment can be done by chemical reactions between reactive groups on the biologically active molecule and reactive groups of the multimerisation domain and/or between reactive groups on the biologically active molecule and reactive groups of the MHC-peptide complex. Alternatively, attachment is done by non- covalent interaction between part of the multimerisation domain and part of the biological active molecule or between part of the MHC-peptide complex and part of the biological active molecule. In both covalent and non-covalent attachment of the biologically molecule to the multimerisation domain a linker molecule can connect the two. The linker molecule can be covalent or non-covalent attached to both molecules. Examples of linker molecules are described elsewhere herein. Some of the MHCmer structures better allows these kind of modifications than others. Biological active molecules can be attached repetitively aiding to recognition by and stimulation of the innate immune system via Toll or other receptors. o

MHC multimers carrying one or more additional groups can be used as therapeutic or vaccine reagents.

In particular, the biologically active molecule may be selected from

proteins such as MHC Class l-like proteins like MIC A, MIC B, CD1 d, HLA E, HLA F, HLA G, HLA H, ULBP-1 , ULBP-2, and ULBP-3,

co-stimulatory molecules such as CD2, CD3, CD4, CD5, CD8, CD9, CD27, CD28, CD30, CD69, CD134 (OX40), CD137 (4-1 BB), CD147, CDw150 (SLAM), CD152

(CTLA-4), CD153 (CD30L), CD40L (CD154), NKG2D, ICOS, HVEM, HLA Class II, PD- 1 , Fas (CD95), FasL expressed on T and/or NK cells, CD40, CD48, CD58, CD70, CD72, B7.1 (CD80), B7.2 (CD86), B7RP-1 , B7-H3, PD-L1 , PD-L2, CD134L, CD137L, ICOSL, LIGHT expressed on APC and/or tumour cells,

cell modulating molecules such as CD16, NKp30, NKp44, NKp46, NKpδO, 2B4, KIR, LIR, CD94/NKG2A, CD94/NKG2C expressed on NK cells, IFN-alpha, IFN-beta, IFN- gamma, IL-1 , IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, IL-10, IL-1 1 , IL-12, IL-15, CSFs (colony- stimulating factors), vitamin D3, IL-2 toxins, cyclosporin, FK-506, rapamycin, TGF-beta, clotrimazole, nitrendipine, and charybdotoxin,

accessory molecules such as LFA-1 , CD1 1 a/18, CD54 (ICAM-1 ), CD106 (VCAM), and CD49a,b,c,d,e,f/CD29 (VLA-4),

adhesion molecules such as ICAM-1 , ICAM-2, GlyCAM-1 , CD34, anti-LFA-1 , anti- CD44, anti-beta7, chemokines, CXCR4, CCR5, anti-selectin L, anti-selectin E, and anti-selectin P,

toxic molecules selected from toxins, enzymes, antibodies, radioisotopes, chemi- luminescent substances, bioluminescent substances, polymers, metal particles, and haptens, such as cyclophosphamide, methrotrexate, Azathioprine, mizoribine, 15- deoxuspergualin, neomycin, staurosporine, genestein, herbimycin A, Pseudomonas exotoxin A, saporin, Rituxan, Ricin, gemtuzumab ozogamicin, Shiga toxin, heavy metals like inorganic and organic mercurials, and FN18-CRM9, radioisotopes such as OO

incorporated isotopes of iodide, cobalt, selenium, tritium, and phosphor, and haptens such as DNP, and digoxiginin,

and combinations of any of the foregoing, as well as antibodies (monoclonal, polyclonal, and recombinant) to the foregoing, where relevant. Antibody derivatives or fragments thereof may also be used.

Design and generation of product to be used for immune monitoring, diagnosis, therapy or vaccination The product of the present invention may be used forjmmune monitoring, diagnosis, therapy and/or vaccination. The generation of product may follow some or all of the following general steps.

1 . Design of antigenic peptides 2. Choice of MHC allele

3. Generation of product

4. Validation and optimization of product

Production of a MHC multimer diagnostic or immune monitoring reagent may follow some or all of the following steps.

1 . Identify disease of interest. Most relevant diseases in this regard are infectious-, cancer-, auto immune-, transplantation-, or immuno-suppression- related diseases.

2. Identify relevant protein antigen(s). This may be individual proteins, a group of proteins from a given tissue or subgroups of proteins from an organism.

3. Identify the protein sequence. Amino acid sequences can be directly found in databases or deduced from gene- or mRNA sequence e.g. using the following link http://www.ncbi.nlm.nih.gov/Genbank/index.html. If not in databases relevant proteins or genes encoding relevant proteins may be isolated and sequenced. In some cases only DNA sequences will be available without knowing which part of the sequence is protein coding. Then the DNA sequence is translated into amino acid sequence in all reading frames.

4. Choose MHC allele(s). Decide on needed MHC allele population coverage. If a broad coverage of a given population is needed (i.e. when generally applicable reagents are sought) the most frequently expressed MHC alleles by the population of interest may be chosen e.g. using the database http://www.allelefrequencies.net/test/default1.asp or http://epitope.liai.org:8080/tools/population/iedb_input.

In case of personalized medicine the patient is tissue typed (HLA type) and then MHC alleles may be selected according to that.

5. Run the general peptide epitope generator program described elsewhere herein on all selected amino acid sequences from step 3, thereby generating all possible epitopes of defined length (8'-, 9'-, 10'-, 1 1 '-, 13-, 14'-, 15'-, and/or 16'- mers). 6. If searching for broadly applicable epitope sequences, a good alternative to step 5 is to run the "intelligent" peptide epitope prediction programs on the selected amino acid sequences of step 3 using the selected MHC alleles from step 4 e.g. using epitope prediction programs like http://www.syfpeithi.de/, http://www.cbs.dtu.dk/services/NetMHC/, and http://www.cbs. dtu.dk/services/NetMHCI I/.

This step can also be used supplementary to step 5 by running selected or all epitopes from the general peptide epitope generator program through one or more of the intelligent peptide epitope prediction programs.

7. If searching for broadly applicable epitope sequences, one may choose to select the epitopes with highest binding score, or the most likely proteolytic products of the species in question, for the chosen MHC alleles and run them through the BLAST program (http://www.ncbi.nlm.nih.gov/blast/Blast.cgi) to validate the uniqueness of the peptides. If the peptide sequences are present in other species, evaluate the potential risk of disease states caused by the non- relevant species in relation to causing false positive results. If considered being a potential problem for evaluating the future analysis outcome, leave out the peptide. Preferably, choose unique peptide sequences only present in the selected protein.

8. Produce selected peptides as described elsewhere herein, e.g. by standard organic synthesis, and optionally test for binding to the desired MHC alleles by e.g in vitro folding, peptide exchange of already preloaded MHC complexes or another method able to test for peptide binding to MHC I or Il molecules.

9. Generate desired MHC multimer by covalently or non-covalently attaching MHC-peptide complex(es) to multimerization domain, and optionally attach a fluorophore to the MHC multimer, as described elsewhere herein. Optionally, test efficacy in detecting specific T-cells using e.g. the methods described in the section "Detection".

The MHC multimer reagents may be used in a diagnostic procedure or kit for testing patient and control samples e.g. by flow cytometry, immune histochemistry, Elispot or other methods as described herein.

Production of a MHC multimer therapeutic reagent may follow some or all of the following steps. 1. As step 1 - 8 above for diagnostic reagent.

9. Select additional molecules (e.g. biologically active molecules, toxins) to attach to the MHC multimer as described elsewhere herein. The additional molecules can have different functionalities as e.g. adjuvants, specific activators, toxins etc. 10. Test the therapeutic reagent following general guidelines

1 1 . Use for therapy

Processes involving MHC multimers

The present invention relates to methods for detecting the presence of MHC recognising cells in a sample comprising the steps of

(a) providing a sample suspected of comprising MHC recognising cells,

(b) contacting the sample with a MHC multimer as defined above, and

(c) determining any binding of the MHC multimer. Binding indicates the presence of MHC recognising cells.

Such methods are a powerful tool in diagnosing various diseases. Establishing a diagnosis is important in several ways. A diagnosis provides information about the disease, thus the patient can be offered a suitable treatment regime. Also, establishing a more specific diagnosis may give important information about a subtype of a disease for which a particular treatment will be beneficial (i.e. various subtypes of diseases may involve display of different peptides which are recognised by MHC recognising cells, and thus treatment can be targeted effectively against a particular subtype). In this way, it may also be possible to gain information about aberrant cells, which emerge through the progress of the disease or condition, or to investigate whether and how T- cell specificity is affected. The binding of the MHC multimer makes possible these options, since the binding is indicative for the presence of the MHC recognising cells in the sample, and accordingly the presence of MHC multimers displaying the peptide.

The present invention also relates to methods for monitoring MHC recognising cells comprising the steps of

(a) providing a sample suspected of comprising MHC recognising cells,

(b) contacting the sample with a MHC complex as defined above, and

(c) determining any binding of the MHC multimer, thereby monitoring MHC recognising cells.

Such methods are a powerful tool in monitoring the progress of a disease, e.g. to closely follow the effect of a treatment. The method can i.a. be used to manage or control the disease in a better way, to ensure the patient receives the optimum treatment regime, to adjust the treatment, to confirm remission or recurrence, and to ensure the patient is not treated with a medicament which does not cure or alleviate the disease. In this way, it may also be possible to monitor aberrant cells, which emerge through the progress of the disease or condition, or to investigate whether and how T- cell specificity is affected during treatment. The binding of the MHC multimer makes possible these options, since the binding is indicative for the presence of the MHC recognising cells in the sample, and accordingly the presence of MHC multimers displaying the peptide.

The present invention also relates to methods for establishing a prognosis of a disease involving MHC recognising cells comprising the steps of (a) providing a sample suspected of comprising MHC recognising cells,

(b) contacting the sample with a MHC multimer as defined above, and

(c) determining any binding of the MHC multimer, thereby establishing a prognosis of a disease involving MHC recognising cells.

Such methods are a valuable tool in order to manage diseases, i.a. to ensure the patient is not treated without effect, to ensure the disease is treated in the optimum way, and to predict the chances of survival or cure. In this way, it may also be possible to gain information about aberrant cells, which emerge through the progress of the disease or condition, or to investigate whether and how T-cell specificity is affected, thereby being able to establish a prognosis. The binding of the MHC multimer makes possible these options, since the binding is indicative for the presence of the MHC recognising cells in the sample, and accordingly the presence of MHC complexs displaying the peptide.

The present invention also relates to methods for determining the status of a disease involving MHC recognising cells comprising the steps of

(a) providing a sample suspected of comprising MHC recognising cells,

(b) contacting the sample with a MHC complex as defined above, and

(c) determining any binding of the MHC complex, thereby determining the status of a disease involving MHC recognising cells.

Such methods are a valuable tool in managing and controlling various diseases. A disease could, e.g. change from one stage to another, and thus it is important to be able to determine the disease status. In this way, it may also be possible to gain information about aberrant cells which emerge through the progress of the disease or condition, or to investigate whether and how T-cell specificity is affected, thereby determining the status of a disease or condition. The binding of the MHC complex makes possible these options, since the binding is indicative for the presence of the MHC recognising cells in the sample, and accordingly the presence of MHC complexs displaying the peptide.

The present invention also relates to methods for the diagnosis of a disease involving MHC recognising cells comprising the steps of (a) providing a sample suspected of comprising MHC recognising cells, (b) contacting the sample with a MHC multimer as defined above, and

(c) determining any binding of the MHC multimer, thereby diagnosing a disease involving MHC recognising cells.

Such diagnostic methods are a powerful tool in the diagnosis of various diseases. Establishing a diagnosis is important in several ways. A diagnosis gives information about the disease, thus the patient can be offered a suitable treatment regime. Also, establishing a more specific diagnosis may give important information about a subtype of a disease for which a particular treatment will be beneficial (i.e. various subtypes of diseases may involve display of different peptides which are recognised by MHC recognising cells, and thus treatment can be targeted effectively against a particular subtype). Valuable information may also be obtained about aberrant cells emerging through the progress of the disease or condition as well as whether and how T-cell specificity is affected. The binding of the MHC multimer makes possible these options, since the binding is indicative for the presence of the MHC recognising cells in the sample, and accordingly the presence of MHC multimers displaying the peptide.

The present invention also relates to methods of correlating cellular morphology with the presence of MHC recognising cells in a sample comprising the steps of (a) providing a sample suspected of comprising MHC recognising cells, (b) contacting the sample with a MHC multimer as defined above, and

(c) determining any binding of the MHC multimer, thereby correlating the binding of the MHC multimer with the cellular morphology.

Such methods are especially valuable as applied in the field of histochemical methods, as the binding pattern and distribution of the MHC multimers can be observed directly. In such methods, the sample is treated so as to preserve the morphology of the individual cells of the sample. The information gained is important i.a. in diagnostic procedures as sites affected can be observed directly.

The present invention also relates to methods for determining the effectiveness of a medicament against a disease involving MHC recognising cells comprising the steps of

(a) providing a sample from a subject receiving treatment with a medicament,

(b) contacting the sample with a as defined herein, and (c) determining any binding of the MHC multimer, thereby determining the effectiveness of the medicament.

Such methods are a valuable tool in several ways. The methods may be used to determine whether a treatment is effectively combating the disease. The method may also provide information about aberrant cells which emerge through the progress of the disease or condition as well as whether and how T-cell specificity is affected, thereby providing information of the effectiveness of a medicament in question. The binding of the MHC multimer makes possible these options, since the binding is indicative for the presence of the MHC recognising cells in the sample, and accordingly the presence of MHC multimers displaying the peptide. The present invention also relates to methods for manipulating MHC recognising cells populations comprising the steps of

(a) providing a sample comprising MHC recognising cells,

(b) contacting the sample with a MHC multimer immobilised onto a solid support as defined above,

(c) isolating the relevant MHC recognising cells, and

(d) expanding such cells to a clinically relevant number, with or without further manipulation.

Such ex vivo methods are a powerful tool to generate antigen-specific, long-lived human effector T-cell populations that, when re-introduced to the subject, enable killing of target cells and has a great potential for use in immunotherapy applications against various types of cancer and infectious diseases.

As used everywhere herein, the term "MHC recognising cells" are intended to mean such which are able to recognise and bind to MHC multimers. The intended meaning of "MHC multimers" is given above. Such MHC recognising cells may also be called MHC recognising cell clones, target cells, target MHC recognising cells, target MHC molecule recognising cells, MHC molecule receptors, MHC receptors, MHC peptide specific receptors, or peptide-specific cells. The term "MHC recognising cells" is intended to include all subsets of normal, abnormal and defect cells, which recognise and bind to the MHC molecule. Actually, it is the receptor on the MHC recognising cell that binds to the MHC molecule.

As described above, in diseases and various conditions, peptides are displayed by means of MHC multimers, which are recognised by the immune system, and cells targeting such MHC multimers are produced (MHC recognising cells). Thus, the presence of such MHC protein recognising cells is a direct indication of the presence of MHC multimers displaying the peptides recognised by the MHC protein recognising cells. The peptides displayed are indicative and may involved in various diseases and conditions. For instance, such MHC recognising cells may be involved in diseases of inflammatory, auto-immune, allergic, viral, cancerous, infectious, allo- or xenogene (graft versus host and host versus graft) origin.

The MHC multimers of the present invention have numerous uses and are a valuable and powerful tool e.g. in the fields of therapy, diagnosis, prognosis, monitoring, stratification, and determining the status of diseases or conditions. Thus, the MHC multimers may be applied in the various methods involving the detection of MHC recognising cells.

Furthermore, the present invention relates to compositions comprising the MHC multimers in a solubilising medium. The present invention also relates to compositions comprising the MHC multimers immobilised onto a solid or semi-solid support.

The MHC multimers can be used in a number of applications, including analyses such as flow cytometry, immunohistochemistry (IHC), and ELISA-like analyses, and can be used for diagnostic, prognostic or therapeutic purposes including autologous cancer therapy or vaccines such as CMV or HIV vaccine or cancer vaccine.

The MHC multimers are very suitable as detection systems. Thus, the present invention relates to the use of the MHC multimers as defined herein as detection systems.

In another aspect, the present invention relates to the general use of MHC peptide complexes and multimers of such MHC peptide complexes in various methods. These methods include therapeutic methods, diagnostic methods, prognostic methods, methods for determining the progress and status of a disease or condition, and methods for the stratification of a patient.

The MHC multimers of the present invention are also of value in testing the expected efficacy of medicaments against or for the treatment of various diseases. Thus, the present invention relates to methods of testing the effect of medicaments or treatments, the methods comprising detecting the binding of the MHC multimers to MHC recognising cells and establishing the effectiveness of the medicament or the treatment in question based on the specificity of the MHC recognising cells. As mentioned above, the present invention also relates generally to the field of therapy. Thus, the present invention relates per se to the MHC multimer as defined herein for use as medicaments, and to the MHC multimers for use in in vivo and ex vivo therapy.

The present invention relates to therapeutic compositions comprising as active ingredients the MHC multimers as defined herein.

An important aspect of the present invention is therapeutic compositions comprising as active ingredients effective amounts of MHC recognising cells obtained using the MHC multimers as defined herein to isolate relevant MHC recognising cells, and expanding such cells to a clinically relevant number.

The present invention further relates to methods for treating, preventing or alleviating diseases, methods for inducing anergy of cells, as well as to methods for up-regulating, down-regulating, modulating, stimulating, inhibiting, restoring, enhancing and/or otherwise manipulating immune responses.

The invention also relates to methods for obtaining MHC recognising cells by using the MHC multimers as described herein.

Also encompassed by the present invention are methods for preparing the therapeutic compositions of the invention.

The present invention is also directed to generating MHC multimers for detecting and analysing receptors on MHC recognising cells, such as epitope specific T-cell clones or other immune competent effector cells.

It is a further object of the present invention to provide new and powerful strategies for the development of curative vaccines. This in turn will improve the possibilities for directed and efficient immune manipulations against diseases caused by tumour genesis or infection by pathogenic agent like viruses and bacteria. HIV is an important example. The ability to generate and optionally attach recombinant MHC multimers to multimerization domains, such as scaffolds and/or carrier molecules, will enable the development of a novel analytical and therapeutical tool for monitoring immune responses and contribute to a rational platform for novel therapy and "vaccine" applications.

Therapeutic compositions (e.g. "therapeutical vaccines") that stimulate specific T-cell proliferation by peptide-specific stimulation is indeed a possibility within the present invention. Thus, quantitative analysis and ligand-based detection of specific T-cells that proliferate by the peptide specific stimulation should be performed simultaneously to monitoring the generated response.

Application of MHC multimers in immune monitoring, diagnostics, therapy, vaccine

MHC multimers as described herein can be used to identify and isolate specific T cells in a wide array of applications. In principle all kind of samples possessing T cells can be analyzed with MHC multimers.

MHC multimers detect antigen-specific T cells of the various T cell subsets. T cells are pivotal for mounting an adaptive immune response. It is therefore of importance to be able to measure the number of specific T cells when performing a monitoring of a given immune response. Typically, the adaptive immune response is monitored by measuring the specific antibody response, which is only one of the effector arms of the immune system. This can lead to miss-interpretation of the actual clinical immune status.

In many cases intruders of the organism can hide away inside the cells, which often does not provoke a humoral response. In other cases, e.g. in the case of certain viruses the intruder mutates fast, particularly in the genes encoding the proteins that are targets for the humoral response. Examples include the influenza and HIV viruses. The high rate of mutagenesis renders the humoral response unable to cope with the infection. In these cases the immune system relies on the cellular immune response. When developing vaccines against such targets one needs to provoke the cellular response in order to get an efficient vaccine.

MHC multimers can be used for monitoring immune responses elicited by vaccines One preferred embodiment of the present invention is monitoring the effect of vaccines against infectious disease, e.g. CMV infection. CMV is a member of the herpes virus familily and is particularly abundant, with 50-80 % of all individuals world-wide being infected. In healthy immuno-competent individuals, CMV infection is asymptomatic. An equilibrium is achieved where CMV-specific T-cells control the persisting virus. However, in AIDS patients, in patients rcieving immune suppressive treatment or in others with compromisedimmune system the latent infections may be reactivated if the immune system is suppressed too much, leading to serious complications and death. Also in young children with not fully developed immune system infection with CMV pose a danger. An affective and save vaccine against CMV are therefore useful to prevent or inhibit CMV infection in e.g. young children. MHC multimers of the present invention can be useful during development of CMV vaccine's and later to monitor the outcome of a vaccination.

In another preferred embodiment of the present invention MHC multimers are used as components of a CMV vaccine. An example of usefull MHC multimers are cells expressing MHC-peptide complexes where the antigenic peptides are derived from proteins of CMV. Such cells if used as a vaccine may be able to induce a cellular immune response generating T cells specific for the protein from which the antigenic peptides are derived and thereby generate an immune respons against the CMV. To further enhance the MHC-peptide specific stimulation of the T cells, T cell stimulatory molecules can be coupled to the multimerisation domain together with MHC or may be added as soluble adjuvant together with the MHC multimer. Example T cell stimulatory molecules include but are not limited to IL-2, CD80 (B7.1 ), CD86 (B7.2), anti-CD28 antibody, CD40, CD37ligand (4-1 BBL), IL-6, IL-15JL-21 , IFN-γ, IFN-α, IFN-β, CD27 ligand, CD30 ligand, IL-23, IL-1 α and IL-1 β. One or more T cell stimulatory molecules may be added together with or coupled to the MHC multimer. Likewise, adjuvants or molecules enhancing or otherwise affecting the cellular, humoral or innate immune response may be coupled to or added together with the MHC multimer vaccine.

Other MHC multimers as described elsewhere herein may also be usefull as vaccines against CMV or other infectious diseases by eliciting pathogen-specific immune responses.

In principles any MHC multimer or derivatives of MHC multimers can be useful as vaccines, as vaccine components or as engineered intelligent adjuvant. The possibility of combining MHC multimers that specifically bind certain T cells with molecules that trigger, e.g. the humoral response or the innate immune response, can accelerate vaccine development and improve the efficiency of vaccines.

The number of antigen-specific cytotoxic T cells can be used as surrogate markers for the overall wellness of the immune system. The immune system can be compromised severely by natural causes such as HIV infections, big traumas, by immuno suppressive therapy in relation to transplantation or due to treatment with chemotherapy. The efficacy of an anti HIV treatment can be evaluated by studying the number of common antigen-specific cytotoxic T cells, specific for e.g. Cytomegalovirus (CMV) and Epstein- Barr virus. In this case the measured T cells can be conceived as surrogate markers. The treatment can then be corrected accordingly and a prognosis can be made.

A similar situation is found for patients undergoing transplantation as they are severely immune compromised due to pharmaceutical immune suppression to avoid organ rejection. The suppression can lead to outbreak of opportunistic infections caused by reactivation of otherwise dormant viruses residing in the transplanted patients or the grafts. This can be the case for CMV and EBV viruses. Therefore measurement of the number of virus-specific T cells can be used to give a prognosis for the outcome of the transplantation and adjustment of the immune suppressive treatment. Similarly, the BK virus has been implied as a causative reagent for kidney rejection. Therefore measurement of BK-virus specific T cells can have prognostic value.

MHC multimers can be of importance in diagnosis of infections caused by bacteria, virus and parasites that hide away inside cells. Serum titers can be very low and direct measurement of the disease-causing organisms by PCR or other methods directly detecting the presence of pathogen can be very difficult because the host cells are not identified or are inaccessible. Other clinical symptoms of a chronical infection can be unrecognizable in an otherwise healthy individuals, even though such persons still are disease-carriers and at risk of becoming spontaneously ill if being compromised by other diseases or stress.

Antigen-specific T helper cells and regulatory T cells have been implicated in the development of autoimmune disorders. In most cases the timing of events leading to autoimmune disease is unknown and the exact role of the immune cells not clear. Use of MHC multimers to study these diseases will lead to greater understanding of the disease-causing scenario and make provisions for development of therapies and vaccines for these diseases.

Therapeutic use of MHC multimers is possible, either directly or as part of therapeutic vaccines. In therapies involving T cells, e.g. treatment with in vitro amplified antigen- specific effector T cells, the T cells often do not home effectively to the correct target sites but ends up in undesired parts of the body. If the molecules responsible for interaction with the correct homing receptor can be identified these can be added to the MHC multimer making a dual, triple or multiple molecular structure that are able to aid the antigen-specific T cells home to the correct target, as the MHC multimer will bind to the specific T cell and the additional molecules will mediate binding to the target cells. In a preferable embodiment, MHC multimers bound to other functional molecules are employed to directly block, regulate or kill the targeted cells.

In another aspect of the present invention modulation of regulatory T cells could be part of a treatment. In diseases where the function of regulatory T cells is understood it may be possible to directly block, regulate or kill these regulatory cells by means of MHC multimers that besides MHC-peptide complexes also features other functional molecules. The MHC multimers specifically recognize the target regulatory T cells and direct the action of the other functional molecules to this target T cell.

Diseases

MHCmers can be used in immune monitoring, diagnostics, prognostics, therapy and vaccines for many different diseases, including but not limited to the diseases listed in the following.

a) Infectious diseases caused by virus such as,

Adenovirus (subgropus A-F), BK-virus, CMV (Cytomegalo virus, HHV-5), EBV (Epstein Barr Virus, HHV-4), HBV (Hepatitis B Virus), HCV (Hepatitis C virus), HHV-6a and b

(Human Herpes Virus-6a and b), HHV-7, HHV-8, HSV- 1 (Herpes simplex virus-1 , HHV- 1 ), HSV-2 (HHV-2), JC-virus, SV-40 (Simian virus 40), VZV (Varizella-Zoster- Virus, HHV-3), Parvovirus B19, Haemophilus influenza, HIV-1 (Human immunodeficiency Virus-1 ), HTLV-1 (Human T-lymphotrophic virus-1 ), HPV (Human Papillomavirus giving rise to clinical manifestions such as Hepatitis, AIDS, Measles, Pox, Chicken pox, Rubella, Herpes and others

b) Infectious diseases caused by bacteria such as, Gram positive bacteria, gram negative bacteria, intracellular bacterium, extracellular bacterium, Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium avium subsp. Paratuberculosis, Mycobacterium africanum, Mycobacterium canetti, Mycobacterium microti, Mycobacterium kansasii, Mycobacterium malmoense, Mycobacterium abscessus, Mycobacterium xenopi, other mycobacteria, Borrelia burgdorferi, other spirochetes, Helicobacter pylori, Streptococcus pneumoniae, Listeria monocytogenes, Histoplasma capsulatum, Bartonella henselae, Bartonella quintana giving rise to clinical manifestations such as Tuberculosis, Pneumonia, Stomach ulcers, Paratuberculosis and others

c) Infectious diseases caused by fungus such as,

Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Pneumocystis carinii giving rise to clinical manifestations such as skin-, nail-, and mucosal infections, Meningitis, Sepsis and others

d) Parasitic diseases caused by parasites such as,

Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Schistosoma mansoni, Schistosoma japonicum, Schistosoma haematobium, Trypanosoma cruzi,

Trypanosoma rhodesiense, Trypanosoma gambiense, Leishmania donovani, and

Leishmania tropica.

e) Allergic diseases caused by allergens such as,

Birch, Hazel, Elm, Ragweed, Wormwood, Grass, Mould, Dust Mite giving rise to clinical manifestations such as Asthma.

f) Transplantation-related diseases caused by reactions to minor histocompatibility antigens such as HA-1 , HA-8, USP9Y, SMCY, TPR-protein, HB-1 Y and other antigens in relation to, Graft-versus-host-related disease, allo- or xenogene reactions i.e. graft-versus-host and host-versus-graft disease. g) Cancerous diseases associated with antigens such as Survivin, Survivin-2B, Livin/ML-IAP, Bcl-2, Mcl-1 , BcI-X(L), Mucin-1 , NY-ESO-1 , Telomerase, CEA, MART-1 , HER-2/neu, bcr-abl, PSA, PSCA, Tyrosinase, p53, hTRT, Leukocyte Proteinase-3, hTRT, gpl OO, MAGE antigens, GASC, JMJD2C, JARD2 (JMJ), JHDM3a, WT- 1 ,CA 9, Protein kinases, where the cancerous diseases include malignant melanoma, renal carcinoma, breast cancer, lung cancer, cancer of the uterus, cervical cancer, prostatic cancer, pancreatic cancer, brain cancer, head and neck cancer, leukemia, cutaneous lymphoma, hepatic carcinoma, colorectal cancer, bladder cancer.

h) Autoimmune and inflammatory diseases, associated with antigens such as GAD64, Collagen, human cartilage glycoprotein 39, β-amyloid, Aβ42, APP, Presenilin 1 , where the autoimmune and inflammatory diseases include Diabetes type 1 , Rheumatoid arthritis, Alzheimer, chronic inflammatory bowel disease, Crohn's disease, ulcerative colitis uterosa, Multiple Sclerosis, Psoriasis

Approaches to the analysis or treatment of diseases.

For each application of a MHC multimer, a number of choices must be made. These include: A. Disease (to be e.g. treated, prevented, diagnosed, monitored).

B. Application (e.g. analyze by flow cytometry, isolate specific cells, induce an immune response)

C. Label (e.g. should the MHC multimer be labelled with a fluorophore or a chromophore) D. Biologically active molecule (e.g. should a biologically active molecule such as an interleukin be added or chemically linked to the complex)

E. Peptide (e.g. decide on a peptide to be complexed with MHC)

F. MHC (e.g. use a MHC allele that does not interfere with the patient's immune system in an undesired way).

A number of diseases A₁-A_n, relevant in connection with MHC multimers, have been described herein; a number of applications B₁-B_n, relevant in connection with MHC multimers, have been described herein; a number of Labels C₁-C_n, relevant in connection with MHC multimers, have been described herein; a number of biologically active molecules D₁-D_n, relevant in connection with MHC multimers, have been described herein; a number of peptides ErE_n, relevant in connection with MHC multimers, have been described herein; and a number of MHC molecules FrF_n, relevant in connection with MHC multimers, have been described herein.

Thus, each approach involves a choice to be made regarding all or some of the parameters A-F. A given application and the choices it involves can thus be described as follows:

Ai x Bi x Ci x Di x Ei x Fi

Where i specifies a number between 1 and n. n is different for different choices A, B, C, D, E, or F. Consequently, the present invention describes a large number of approaches to the diagnosis, monitoring, prognosis, therapeutic or vaccine treatment of diseases. The total number of approaches, as defined by these parameters, are

n(A) x n(B) x n(C) x n(D) x n(E) x n(F),

where n(A) describes the number of different diseases A described herein, n(B) describes the number of different applications B described herein, etc.

Detection

Diagnostic procedures, immune monitoring and some therapeutic processes all involve identification and/or enumeration and/or isolation of antigen-specific T cells. Identification and enumeration of antigen-specific T cells may be done in a number of ways, and several assays are currently employed to provide this information. In the following it is described how MHC multimers as described in the present invention can be used to detect specific T cell receptors (TCRs) and thereby antigen- specific T cells in a variety of methods and assays. In the present invention detection includes detection of the presence of antigen-specific T cell receptors/ T cells in a sample, detection of and isolation of cells or entities with antigen-specific T cell receptor from a sample and detection and enrichment of cells or entities with antigen- specific T cell receptor in a sample.

The sample may be a biological sample including solid tissue, solid tissue section or a fluid such as, but not limited to, whole blood, serum, plasma, nasal secretions, sputum, urine, sweat, saliva, transdermal exudates, pharyngeal exudates, bronchoalveolar lavage, tracheal aspirations, cerebrospinal fluid, synovial fluid, fluid from joints, vitreous fluid, vaginal or urethral secretions, or the like. Herein, disaggregated cellular tissues such as, for example, hair, skin, synovial tissue, tissue biopsies and nail scrapings are also considered as biological samples.

Many of the assays are particularly useful for assaying T-cells in blood samples. Blood samples are whole blood samples or blood processed to remove erythrocytes and platelets (e.g., by Ficoll density centrifugation or other such methods known to one of skill in the art) and the remaining PBMC sample, which includes the T-cells of interest, as well as B-cells, macrophages and dendritic cells, is used directly.

In order to be able to detect specific T cells by MHC multimers, labels and marker molecules can be used.

Marker molecules Marker molecules are molecules or complexes of molecules that bind to other molecules. Marker molecules thus may bind to molecules on entities, including the desired entities as well as undesired entities. Labeling molecules are molecules that may be detected in a certain analysis, i.e. the labeling molecules provide a signal detectable by the used method. Marker molecules, linked to labeling molecules, constitute detection molecules. Likewise labeling molecules linked to MHC multimers also constitute detection molecules but in contrast to detection molecules made up of marker and lebelling molecule labeled MHC multimers are specific for TCR. Sometimes a marker molecule in itself provides a detectable signal, wherefore attachment to a labeling molecule is not necessary. Marker molecules are typically antibodies or antibodyfragments but can also be aptamers, proteins, peptides, small organic molecules, natural compounds (e.g. steroids), non-peptide polymers, or any other molecules that specifically and efficiently bind to other molecules are also marker molecules.

Labelling molecules Labelling molecules are molecules that can be detected in a certain analysis, i.e. the labelling molecules provide a signal detectable by the used method. The amount of labelling molecules can be quantified.

The labelling molecule is preferably such which is directly or indirectly detectable. The labelling molecule may be any labelling molecule suitable for direct or indirect detection. By the term "direct" is meant that the labelling molecule can be detected per se without the need for a secondary molecule, i.e. is a "primary" labelling molecule. By the term "indirect" is meant that the labelling molecule can be detected by using one or more "secondary" molecules, i.e. the detection is performed by the detection of the binding of the secondary molecule(s) to the primary molecule.

The labelling molecule may further be attached via a suitable linker. Linkers suitable for attachment to labelling molecules would be readily known by the person skilled in the art and as described elsewhere herein for attachment of MHC molecules to multimerisation domains.

Examples of such suitable labelling compounds are fluorescent labels, enzyme labels, radioisotopes, chemiluminescent labels, bioluminescent labels, polymers, metal particles, haptens, antibodies, and dyes.

The labelling compound may suitably be selected:

from fluorescent labels such as 5-(and 6)-carboxyfluorescein, 5- or 6-carboxy- fluorescein, 6-(fluorescein)-5-(and 6)-carboxamido hexanoic acid, fluorescein isothio- cyanate (FITC), rhodamine, tetramethylrhodamine, and dyes such as Cy2, Cy3, and Cy5, optionally substituted coumarin including AMCA, PerCP, phycobiliproteins including R-phycoerythrin (RPE) and allophycoerythrin (APC), Texas Red, Princeston Red, Green fluorescent protein (GFP) and analogues thereof, and conjugates of R- phycoerythrin or allophycoerythrin and e.g. Cy5 or Texas Red, and inorganic fluorescent labels based on semiconductor nanocrystals (like quantum dot and Qdot™ nanocrystals), and time-resolved fluorescent labels based on lanthanides like Eu3+ and Sm3+,

from haptens such as DNP, biotin, and digoxiginin,

from enzymic labels such as horse radish peroxidase (HRP), alkaline phosphatase (AP), beta-galactosidase (GAL), glucose-6-phosphate dehydrogenase, beta-N-acetyl- glucosaminidase, β-glucuronidase, invertase, Xanthine Oxidase, firefly luciferase and glucose oxidase (GO),

from luminiscence labels such as luminol, isoluminol, acridinium esters, 1 ,2-dioxetanes and pyridopyridazines, and

from radioactivity labels such as incorporated isotopes of iodide, cobalt, selenium, tritium, and phosphor.

Radioactive labels may in particular be interesting in connection with labelling of the peptides harboured by the MHC multimers.

Different principles of labelling and detection exist, based on the specific property of the labelling molecule. Examples of different types of labelling are emission of radioactive radiation (radionuclide, isotopes), absorption of light (e.g. dyes, chromophores), emission of light after excitation (fluorescence from fluorochromes), NMR (nuclear magnetic resonance form paramagnetic molecules) and reflection of light (scatter from e.g. such as gold-, plastic- or glass -beads/particles of various sizes and shapes). Alternatively, the labelling molecules can have an enzymatic activity, by which they catalyze a reaction between chemicals in the near environment of the labelling molecules, producing a signal, which include production of light (chemi-luminescence), precipitation of chromophor dyes, or precipitates that can be detected by an additional layer of detection molecules. The enzymatic product can deposit at the location of the enzyme or, in a cell based analysis system, react with the membrane of the cell or diffuse into the cell to which it is attached. Examples of labelling molecules and associated detection principles are shown in table 2 below.

Table 2: Examples of labelling molecules and associated detection principles.

"Photometry; is to be understood as any method that can be applied to detect the intensity, analyze the wavelength spectra, and or measure the accumulation of light derived form a source emitting light of one or multiple wavelength or spectra.

Labelling molecules can be used to label MHC multimers as well as other reagents used together with MHC multimers, e.g. antibodies, aptamers or other proteins or molecules able to bind specific structures in another protein, in sugars, in DNA or in other molecules. In the following molecules able to bind a specific structure in another molecule are named a marker.

Labelling molecules can be attached to a given MHC multimer or any other protein marker by covalent linkage as described for attachment of MHC multimers to multimerization domains elsewhere herein. The attachment can be directly between o

reactive groups in the labelling molecule and reactive groups in the marker molecule or the attachment can be through a linker covalently attached to labelling molecule and marker, both as described elsewhere herein. When labelling MHC multimers the label can be attached either to the MHC complex (heavy chain, β2m or peptide) or to the multimerization domain.

In particular, one or more labelling molecules may be attached to the carrier molecule, or one or more labelling molecules may be attached to one or more of the scaffolds, or one or more labelling compounds may be attached to one or more of the MHC complexes, or one or more labelling compounds may be attached to the carrier molecule and/or one or more of the scaffolds and/or one or more of the MHC complexes, or one or more labelling compounds may be attached to the peptide harboured by the MHC molecule.

A single labelling molecule on a marker does not always generate sufficient signal intensity. The signal intensity can be improved by assembling single label molecules into large multi-labelling compounds, containing two or more label molecule residues. Generation of multi-label compounds can be achived by covalent or non-covalent, association of labelling molecules with a major structural molecule. Examples of such structures are synthetic or natural polymers (e.g. dextramers), proteins (e.g. streptavidin), or polymers. The labelling molecules in a multi-labelling compound can all be of the same type or can be a mixture of different labelling molecules.

In some applications, it may be advantageous to apply different MHC complexs, either as a combination or in individual steps. Such different MHC multimers can be differently labelled (i.e. by labelling with different labelling compounds) enabling visualisation of different target MHC recognising cells. Thus, if several different MHC multimers with different labelling compounds are present, it is possible simultaneously to identify more than one specific receptor, if each of the MHC multimers present a different peptide.

Detection principles, such as listed in Table 2, can be applied to flow cytometry, stationary cytometry, and batch-based analysis. Most batch-based approaches can use any of the labelling substances depending on the purpose of the assay. Flow cytometry primarily employs fluorescence, whereas stationary cytometry primarily employs light absorption, e.g. dyes or chromophore deposit from enzymatic activity. In the following section, principles involving fluorescence detection will be exemplified for flow cytometry, and principles involving chromophore detection will be exemplified in the context of stationary cytometry. However, the labelling molecules can be applied to any of the analyses described in this invention.

Labelling molecules of particular utility in Flow Cytometry:

In flowcytometry the typical label is detected by its fluorescence. Most often a positive detection is based on the presents of light from a single fluorochrome, but in other techniques the signal is detected by a shift in wavelength of emitted light; as in FRET based techniques, where the exited fluorochrome transfer its energy to an adjacent bound fluorochrome that emits light, or when using Ca²⁺ chelating fluorescent props, which change the emission (and absorption) spectra upon binding to calcium. Preferably labelling molecules employed in flowcytometry are illustrated in Table 3 and 4 and described in the following.

Simple fluorescent labels:

• Fluor dyes, Pacific Blue™, Pacific Orange™, Cascade Yellow™,

• AlexaFluor@(AF); o AF405, AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750, AF800

• Quantum Dot based dyes, QDot® Nanocrystals (Invitrogen, MolecularProbs) o Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800

• DyLight™ Dyes (Pierce) (DL); o DL549, DL649, DL680, DL800

• Fluorescein (Flu) or any derivate of that, ex. FITC

• Cy-Dyes o Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7

• Fluorescent Proteins; o RPE, PerCp, APC o Green fluorescent proteins;

^■ GFP and GFP-derived mutant proteins; BFP,CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine, mStrawberry, mCherry • Tandem dyes: o RPE-Cy5, RPE-Cy5.5, RPE-Cy7, RPE-AlexaFluor® tandem conjugates; RPE-Alexa610, RPE-TxRed o APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5, APC-Cy5.5

• lonophors; ion chelating fluorescent props o Props that change wavelength when binding a specific ion, such as

Calcium

Props that change intensity when binding to a specific ion, such as Calcium • Combinations of fluorochromes on the same marker. Thus, the marker is not identified by a single fluorochrome but by a code of identification being a specific combination of fluorochromes, as well as inter related ratio of intensities.

Example: Antibody Ab1 and Ab2, are conjugated to both. FITC and BP but Ab1 have 1 FITC to 1 BP whereas Ab2 have 2 FITC to 1 BP. Each antibodymay then be identified individually by the relative intensity of each fluorochrome. Any such combinations of n fluorochromes with m different ratios can be generated.

• Table 3: Examples of preferable fluorochromes

Table 4: Examples of preferable fluorochrome families Fluorochrome family Example fluorochrome

AlexaFluor®(AF) AFΘ350, AF405, AF430, AF488.AF500, AF514, AF532,

AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750, AF800

Quantum Dot (Qdot®) based Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, dyes Qdot®705, Qdot®800

DyLight™ Dyes (DL) DL549, DL649, DL680, DL800 Small fluorescing dyes FITC, Pacific Blue™, Pacific Orange™, Cascade Yellow™,

Marina blue™, DSred, DSred-2, 7-AAD, TO-Pro-3,

Cy- Dyes Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7 Phycobili Proteins: R-Phycoerythrin (RPE), PerCP, Allophycocyanin (APC), B-

Phycoerythrin, C-Phycocyanin

Fluorescent Proteins (E)GFP and GFP ((enhanced) green fluorescent protein) derived mutant proteins; BFP, CFP, YFP, DsRed, T1 , Dimer2, mRFP1 , M Banana, mOrange, dTomato, tdTomato, mTangerine, mStrawberry, mCherry

Tandem dyes with RPE RPE-Cy5, RPE-Cy5.5, RPE-Cy7, RPE-AlexaFluor® tandem conjugates; RPE-Alexa610, RPE-TxRed

Tandem dyes with APC APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5,

APC-Cy5.5

Calcium dyes Indo-1 -Ca2+ lndo-2-Ca2+ Preferably labelling molecules employed in stationary cytometry and IHC

• Enzymatic labelling, as exemplified in Table 5: o Horse radish peroxidase; reduces peroxides (H₂O₂), and the signal is generated by the Oxygen acceptor when being oxidized.

^■ Precipitating dyes; Dyes that when they arereduced they are soluble, and precipitate when oxidized, generating a coloured deposit at the site of the reaction.

^■ Precipitating agent, carrying a chemical residue, a hapten, for second layer binding of marker molecules, for amplification of the primary signal.

^■ Luminol reaction, generating a light signal at the site of reaction. o Other enzymes, such as Alkaline Phosphatase, capable of converting a chemical compound from a non-detectable molecule to a precipitated detectable molecule, which can be coloured, or carries a hapten as described above.

• Fluorescent labels, as exemplified in Table 3 and 4; as those described for Flow cytometry are likewise important for used in stationary cytometry, such as in fluorescent microscopy.

Table 5: Examples of preferable labels for stationary cytometry

Detection methods and principles

Detection of TCRs with multimers may be direct or indirect.

Direct detection Direct detection of TCRs is detection directly of the binding interaction between the specific T cell receptor and the MHC multimer. Direct detection includes detection of TCR when TCR is attached to lipid bilayer, when TCR is attached to or in a solid medium or when TCR is in solution.

Direct detection of TCR attached to lipid bilayer

One type of TCRs to detect and measure are TCRs attached to lipid bilayer including but is not limited to naturally occurring T cells (from blood, spleen, lymphnode, brain or any other tissue containing T cells), TCR transfected cells, T cell hybridomas, TCRs embedded in liposomes or any other membrane structure. In the following methods for direct detection of entities of TCRs attached to lipid bilayer will be described and any entity consisting of TCR attached to lipid bilayer will be referred to as T cells.

T cells can be directly detected either when in a fluid solution or when immobilized to a support.

Direct detection of T cells in fluid sample.

T cells can be detected in fluid samples as described elsewhere herein and in suspension of disrupted tissue, in culture media, in buffers or in other liquids.T cells in fluid samples can be detected individually or deteced as populations of T cells. In the following different methods for direct detction of T cells in fluid samples are shown.

Direct detection of individual T cells o Direct detection of individual T cells using flow cytometry.

A suspension of T cells are added MHC multimers, the sample washed and then the amount of MHC multimer bound to each cell are measured. Bound

MHC multimers may be labelled directly or measured through addition of labelled marker molecules. The sample is analyzed using a flow cytometer, able to detect and count individual cells passing in a stream through a laser beam. For identification of specific T cells using MHC multimers, cells are stained with fluorescently labeled MHC multimer by incubating cells with MHC multimer and then forcing the cells with a large volume of liquid through a nozzle creating a stream of spaced cells. Each cell passes through a laser beam and any fluorochrome bound to the cell is excited and thereby fluoresces. Sensitive photomultipliers detect emitted fluorescence, providing information about the amount of MHC multimer bound to the cell. By this method MHC multimers can be used to identify specific T cell populations in liquid samples such as synovial fluid or blood.

When analyzing blood samples whole blood can be used with or without lysis of red blood cells. Alternatively lymphocytes can be purified before flow cytometry analysis using standard procedures like a Ficoll-Hypaque gradient. Another possibility is to isolate T cells from the blood sample for example by binding to antibody coated plastic surfaces, followed by elution of bound cells. This purified T cell population can then be used for flow cytometry analysis together with MHC multimers. Instead of actively isolating T cells unwanted cells like B cells and NK cells can be removed prior to the analysis. One way to do this is by affinity chromatography using columns coated with antibodies specific for the unwanted cells. Alternatively, specific antibodies can be added to the blood sample together with complement, thereby killing cells recognized by the antibodies. Various gating reagents can be included in the analysis. Gating reagents here means labeled antibodies or other labeled markers identifying subsets of cells by binding to unique surface proteins. Preferred gating reagents when using MHC multimers are antibodies directed against CD3, CD4, and CD8 identifying major subsets of T cells. Other preferred gating reagents are antibodies against CD14, CD15, CD19, CD25, CD56, CD27, CD28, CD45, CD45RA, CD45RO,

CCR7, CCR5, CD62L, Foxp3 recognizing specific proteins unique for different lymphocytes of the immune system.

Following labelling with MHC multimers and before analysis on a flow cytometer stained cells can be treated with a fixation reagent like formaldehyde to cross- link bound MHC multimer to the cell surface. Stained cells can also be analyzed directly without fixation.

The number of cells in a sample can vary. When the target cells are rare, it is preferable to analyze large amounts of cells. In contrast, fewer cells are required when looking at T cell lines or samples containing many cells of the target cell type.

The flow cytometer can be equipped to separate and collect particular types of cells. This is called cell sorting. MHC multimers in combination with sorting on a flowcytometer can be used to isolate specific T cell populations. Isolated specific T cell populations can then be expanded in vitro. This can be useful in autologous cancer therapy.

Direct determination of the concentration of MHC-peptide specific T cells in a sample can be obtained by staining blood cells or other cell samples with MHC multimers and relevant gating reagents followed by addition of an exact amount of counting beads of known concentration. Counting beads is here to be understood as any fluorescent bead with a size that can be visualized by flow cytometry in a sample containing T cells. The beads could be made of polystyrene with a size of about 1 -10 μm. They could also be made of agarose, polyacrylamide, silica, or any other material, and have any size between 0,1 μm and 100 m. The counting beads are used as reference population to measure the exact volume of analyzed sample. The sample are analyzed on a flow cytometer and the amount of MHC-specific T cell determined using a predefined gating strategy and then correlating this number to the number of counted counting beads in the same sample using the following equation: Amounts of MHC-peptide specific T cells in a blood sample can be determined by flow cytometry by calculating the amount of MHC'mer labeled cells in a given volumen of sample with a given cell density and then back calculate. Exact enumeration of specific T cells is better achieved by staining with MHC'mers together with an exact amount of counting beads followed by flow cytometry analysis. The amount of T cells detected can then be correlated with the amount of counting beads in the same volume of the sample and an exact number of MHC-peptide specific T cells determined:

Concentration of MHC-specific T-cell in sample = (number of MHC-peptide specific T cells counted/number of counting beads counted) x concentration of counting beads in sample

Direct detection of individual T cells in fluid sample by microscopy A suspension of T cells are added MHC multimers, the sample washed and then the amount of MHC multimer bound to each cell are measured. Bound MHC multimers may be labelled directly or measured through addition of labelled marker molecules. The sample is then spread out on a slide or similar in a thin layer able to distinguish individual cells and labelled cells identified using a microscope. Depending on the type of label different types of microscopes may be used, e.g. if fluorescent labels are used a fluorescent microscope is used for the analysis. For example MHC multimers can be labeled with a flourochrome or bound MHC multimer detected with a fluorescent antibody. Cells with bound fluorescent MHC multimers can then be visualized using an immunofluorescence microscope or a confocal fluorescence microscope.

o Direct detection of individual T cells in fluid sample by capture on solid support followed by elution.

MHC multimers are immobilized to a support e.g. beads, immunotubes, wells of a microtiterplate, CD, mircrochip or similar and as decribed elsewhere herein, then a suspension of T cells are added allowing specific T cells to bind MHC multimer molecules. Following washing bound T cells are recovered/eluted (e.g. using acid or competition with a competitor molecules) and counted.

Direct detection of populations of T cells o Cell suspensions are added labeled MHC multimer, samples are washed and then total signal from label are measured. The MHC multimers may be labeled themselves or detected through a labeled marker molecule.

o Cell suspensions are added labeled MHC multimer, samples are washed and then signal from label are amplified and then total signal from label and/or amplifier are measured.

Direct detection of immobilized T cells.

T cells may be immobilized and then detected directly. Immobilization can be on solid support, in solid tissue or in fixator (e.g. paraffin, a sugar matrix or another medium fixing the T cells). Direct detection of T cells immobilized on solid support.

In a number of applications, it may be advantageous immobilise the T cell onto a solid or semi-solid support. Such support may be any which is suited for immobilisation, separation etc. Non-limiting examples include particles, beads, biodegradable particles, sheets, gels, filters, membranes (e. g. nylon membranes), fibres, capillaries, needles, microtitre strips, tubes, plates or wells, combs, pipette tips, micro arrays, chips, slides, or indeed any solid surface material. The solid or semi-solid support may be labelled, if this is desired. The support may also have scattering properties or sizes, which enable discrimination among supports of the same nature, e.g. particles of different sizes or scattering properties, colour or intensities.

Conveniently the support may be made of glass, silica, latex, plastic or any polymeric material. The support may also be made from a biodegradable material.

Generally speaking, the nature of the support is not critical and a variety of materials may be used. The surface of support may be hydrophobic or hydrophilic.

Preferred are materials presenting a high surface area for binding of the T cells. Such supports may be for example be porous or particulate e.g. particles, beads, fibres, webs, sinters or sieves. Particulate materials like particles and beads are generally preferred due to their greater binding capacity. Particularly polymeric beads and particles may be of interest.

Conveniently, a particulate support (e.g. beads or particles) may be substantially spherical. The size of the particulate support is not critical, but it may for example have a diameter of at least 1 μm and preferably at least 2 μm, and have a maximum diameter of preferably not more than 10 μm and more preferably not more than 6 μm. For example, particulate supports having diameters of 2.8 μm and 4.5 μm will work well.

An example of a particulate support is monodisperse particles, i.e. such which are substantially uniform in size (e. g. size having a diameter standard deviation of less than 5%). Such have the advantage that they provide very uniform reproducibility of reaction. Monodisperse particles, e.g. made of a polymeric material, produced by the technique described in US 4,336,173 (ref. 25) are especially suitable. Non-magnetic polymer beads may also be applicable. Such are available from a wide range of manufactures, e.g. Dynal Particles AS, Qiagen, Amersham Biosciences, Serotec, Seradyne, Merck, Nippon Paint, Chemagen, Promega, Prolabo, Polysciences, Agowa, and Bangs Laboratories.

Another example of a suitable support is magnetic beads or particles. The term "magnetic" as used everywhere herein is intended to mean that the support is capable of having a magnetic moment imparted to it when placed in a magnetic field, and thus is displaceable under the action of that magnetic field. In other words, a support comprising magnetic beads or particles may readily be removed by magnetic aggregation, which provides a quick, simple and efficient way of separating out the beads or particles from a solution. Magnetic beads and particles may suitably be paramagnetic or superparamagnetic. Superparamagnetic beads and particles are e.g. described in EP 0 106 873 (Sintef, ref. 26). Magnetic beads and particles are available from several manufacturers, e.g. Dynal Biotech ASA (Oslo, Norway, previously Dynal AS, e.g. Dynabeads^®).

The support may suitably have a functionalised surface. Different types of functionalisation include making the surface of the support positively or negatively charged, or hydrophilic or hydrophobic. This applies in particular to beads and particles. Various methods therefore are e.g. described in US 4,336,173 (ref. 25), US 4,459,378 (ref. 27) and US 4,654,267 (ref. 28).

Immobilized T cells may be detected in several ways including:

o Direct detection of T cells directly immobilized on solid support.

T cells may be directly immobilized on solid support e.g. by non-specific adhesion. Then MHC multimers are added to the immobilized T cells thereby allowing specific T cells to bind the MHC multimers. Bound MHC multimer may be measured through label directly attached to the multimer or through labeled marker molecules. Individual T cells may be detected if the method for analysis is able to distinguish individual labelled cells, e.g. cells are immobilized in a monolayer on a cell culture well or a glass slide. Following staining with labelled multimer a digital picture is taken and labelled cells identified and counted. Alternatively a population of T cells is detected by measurement of total signal from all labelled T cells, e.g. cells are plated to wells of a microtiter plate, stained with labelled MHC multimer and total signal from each well are measured.

o Direct detection of T cells immobilized on solid support through linker molecule T cells can also be immobilized to solid support through a linker molecule. The linker molecule can be an antibody specific for the T cell, a MHC multimer, or any molecule capable of binding T cells. In any case the linker may be attached directly to the solid support, to the solid support through another linker, or the linker may be embedded in a matrix, e.g. a sugar matrix. Then MHC multimers are added to the immobilized T cells thereby allowing specific T cells to bind the MHC multimers. Bound MHC multimer may be measured through label directly attached to the multimer or through labeled marker molecules. Individual T cells may be detected if the method for analysis is able to distinguish individual labelled cells, e.g. a digital picture is taken and labelled cells identified and counted.

By using a specific MHC multimer both for the immobilization of the T-cells and for the labelling of immobilized cells (e.g. by labelling immobilized cells with chromophore- or fluorophore-labelled MHC multimer), a very high analytical specificity may be achieved because of the low background noise that results.

Alternatively a population of T cells is detected by measurement of total signal from all labeled T cells.

o lmmuno profiling: Phenotyping T cell sample using MHC multimer beads or arrays.

Different MHC multimers are immobilized to different beads with different characteristics (e.g. different size, different fluorophores or different fluorescence intensities) where each kind of bead has a specific type of MHC multimer molecule immobilized. The immobilization may be direct or through a linker molecule as described above. The amount of bound T cells to a specific population of beads can be analyzed, thereby phenotyping the sample. The TCR on the T cell is defined by the MHC multimer and hence the bead to which it binds.

Likewise, MHC multimers can be immobilized in an array, e.g. on a glass plate or pin array so that the position in the array specifies the identity of the MHC multimer. Again, the immobilization may be direct or through a linker molecule as described above. After addition of T cells, the amount of bound T cells at a specified position in the array can be determined by addition of a label or labelled marker that binds to cells in general, or that binds specifically to the cells of interest. For example, the cells may be generally labelled by the addition of a labelled molecule that binds to all kinds of cells, or specific cell types, e.g. CD4+ T-cells, may be labelled with anti-CD4 antibodies that are labelled with e.g. a chromophore or fluorophore. Either of these approaches allow a phenotyping of the sample.

• Profiling of an individual's disease-specific T-cell repertoire. Mass profiling of the T-cells of an individual may be done by first immobilizing specific MHC multimers (e.g. 10-10⁶ different MHC multimers, each comprising a specific MHC-peptide combination) in an array (e.g. a glass plate), adding e.g. a blood sample from the individual, and then after washing the unbound cells off, label the immobilized cells. Positions in the array of particularly high staining indicate MHC-peptide combinations that recognize specific T-cells of particularly high abundance or affinity. Thus, an immuno profiling of the individual with regard to the tested MHC-peptide combinations is achieved. A similar profiling of an individuals disease may be made using MHC multimers immobilized to different beads as described above.

Whether the profiling is performed using beads or arrays, the profiling may entail a number of diseases, a specific disease, a set of specific antigens implicated in one or more diseases, or a specific antigen (e.g. implicated in a specific disease or set of diseases).

In a preferred embodiment, an individual's immuno profile for a particular antigen is obtained. Thus, peptides corresponding to all possible 8'-, 9'- 10'- and 1 1 '-mer peptide sequences derived from the peptide antigen sequence are generated, for example by standard organic synthesis or combinatorial chemistry, and the corresponding MHC multimers are produced, using one or more of the class I MHC-alleles of the individual in question. Further, peptides of e.g. 13, 14, 15, 16 and upto 25 amino acids length may be generated, for example by organic synthesis or combinatorial chemistry, corresponding to all

13', 14', 15', 16' and upto 25'-mers of the antigen, and the corresponding class Il MHC multimers are produced, using one or more of the class Il MHC-alleles of the individual in question. For a complete profiling for this particular antigen, all of the HLA-alleles of the individual in question should be used for the generation of the array; i.e., if the HLA class I haplotype of the individual is

HLA-A^*02, HLA-A^*03, HLA-B^*08 and HLA-B^*07, all these HLA class I alleles should be combined with every tested peptide and similarly for all HLA class Il alleles of the given individual. Based on the profile, a personalized drug, -vaccine or -diagnostic test may be produced.

The principle described above may also be employed to distinguish between the immune response raised against a disease (e.g. an infection with a bacterium or the formation of a tumour), and the immune response raised against a vaccine for the same disease (in the example, a vaccine against the bacterium or the tumour). Most vaccines consists of subcomponents of the pathogen /tumour they are directed against and/or are designed to elicit an immune response different from the natural occuring immune response i.e. the T cell epitopes of the two immune reponses differs. Thus, by establishing the immuno profile, using a comprehensive array (i.e. an array that comprises all possible epitopes from one or more antigen(s)) or a subset of these epitopes, it is possible to deduce whether the immune response has been generated against the disease or the vaccine, or against both the disease and the vaccine. If the vaccine raises a response against a particular epitope or a particular set of epitopes, the corresponding positions in the array will give rise to high signals (compared to the remaining positions). Similarly a natural generated immune response will be directed against other and/or more particular epitopes and therefore give rise to high signals in other positions and/or more positions in the array. When an individual is vaccinated the immuno profile will reflect the effect of the vaccination on the immune response, and even if the individual has encountered the disease before and has generated a general immune response towards this disease, it will still be possible to deduce from the profiling whether this individual also has generated a specific response against the vaccine.

In another preferred embodiment, an individual's immuno profile for a set of antigens implicated in a specific disease is obtained. A subset of epitopes from a number of antigens is used. Thus, this is not a comprehensive profiling of this individual with regard to these antigens, but careful selection of the epitopes used may ensure that the profiling data can be used afterwards to choose between e.g. a limited set of vaccines available, or the data can be used to evaluate the immune response of the individual following an infection, where the epitopes used have been selected in order to avoid interference from related infectious diseases. As above, a personalized drug, -vaccine or -diagnostic test may be produced, based on the information obtained from the immuno profiling.

In yet another preferred embodiment, the array comprising all possible 8'-, 9'- 10'- and 1 1 '-mer peptide sequences derived from a given peptide antigen, and all 13, 14, 15 and 16'-mers of the same antigen, are synthesized and assembled in MHC multimers, and immobilized in an array. Then, the ability of the individual peptide to form a complex with MHC is tested. As an example, one may add labelled W6/32 antibody, an antibody that binds correctly folded MHC I heavy chain, when this heavy chain is assembled together with antigenic peptide and beta2microglobulin, and which therefore can be used to detect formation of MHC-peptide complex, as binding of W6/32 antibody is usually considered a strong indication that the MHC-peptide complex has been formed. The ability of different peptides to enter into a MHC-peptide complex may also be promoted by the addition to the array of T-cells. Specific T-cells will drive the formation of the corresponding specific MHC-peptide complexes.

Thus, after addition of T-cells to the array, the MHC-peptide complex integrity can be examined by addition of the labelled W6/32 antibody or other antibodies specific for correct conformation. Positions on the array that have strong signals indicate that the peptide that was added to MHC and immobilized at this position, was capable of forming the MHC-peptide complex in the presence of specific T-cells. Alternatively, the binding of the specific T-cells to the corresponding MHC-peptide complexes may be detected directly through a labbelled antibody specific for the T cell.

Direct detection of immobilized T cells followed by sorting

T cells immobilized to solid support in either of the ways described above can following washing be eluted from the solid support and treated further. This is a method to sort out specific T cells from a population of different T cells. Specific T-cells can e.g. be isolated through the use of bead-based MHC multimers. Bead-based MHC multimers are beads whereto monomer MHC-peptide complexes or MHC multimers are immobilized. After the cells have been isolated they can be manipulated in many different ways. The isolated cells can be activated (to differentiate or proliferate), they can undergo induced apoptosis, or undesired cells of the isolated cell population can be removed. Then, the manipulated cell population can be re-introduced into the patient, or can be introduced into another patient.

A typical cell sorting experiment, based on bead-based MHC multimers, would follow some of the steps of the general procedure outlined in general terms in the following: Acquire the sample, e.g. a cell sample from the bone marrow of a cancer patient. Block the sample with a protein solution, e.g. BSA or skim milk. Block the beads coated with MHC complexes, with BSA or skim milk. Mix MHC-coated beads and the cell sample, and incubate. Wash the beads with washing buffer, to remove unbound cells and non-specifically bound cells. Isolate the immobilized cells, by either cleavage of the linker that connects MHC complex and bead; or alternatively, release the cells by a change in pH, salt- concentration addition of competitive binder or the like. Preferably, the cells are released under conditions that do not disrupt the integrity of the cells. Manipulate the isolated cells (induce apoptosis, proliferation or differentiation)

Direct detection of T cells in solid tissue. o Direct detection of T cells in solid tissue in vitro.

For in vitro methods of the present invention solid tissue includes tissue, tissue biopsies, frozen tissue or tissue biopsies, paraffin embedded tissue or tissue biopsies and sections of either of the above mentioned. In a preferred method of this invention sections of fixed or frozen tissues are incubated with MHC multimer, allowing MHC multimer to bind to specific T cells in the tissue section. The MHC multimer may be labeled directly or through a labeled marker molecule. As an example, the MHC multimer can be labeled with a tag that can be recognized by e.g. a secondary antibody, optionally labeled with HRP or another label. The bound MHC multimer is then detected by its fluorescence or absorbance (for fluorophore or chromophore), or by addition of an enzyme- labeled antibody directed against this tag, or another component of the MHC multimer (e.g. one of the protein chains, a label on the multimerization domain). The enzyme can be Horse Raddish Peroxidase (HRP) or Alkaline Phosphatase (AP), both of which convert a colorless substrate into a colored reaction product in situ. This colored deposit identifies the binding site of the MHC multimer, and can be visualized under a light microscope. The MHC multimer can also be directly labeled with e.g. HRP or AP, and used in IHC without an additional antibody. The tissue sections may derive from blocks of tissue or tissue biopsies embedded in paraffin, and tissue sections from this paraffin-tissue block fixed in formalin before staining. This procedure may influence the structure of the TCR in the fixed T cells and thereby influence the ability to recognize specific MHC complexes. In this case, the native structure of TCR needs to be at least partly preserved in the fixed tissue. Fixation of tissue therefore should be gentle.

Alternatively, the staining is performed on frozen tissue sections, and the fixation is done after MHC multimer staining.

o Direct detection of T cells in solid tissue in vivo For in vivo detection of T cells labeled MHC multimers are injected in to the body of the individual to be investigated. The MHC multimers may be labeled with e.g. a paramagnetic isotope. Using a magnetic resonance imaging (MRI) scanner or electron spin resonance (ESR) scanner MHC multimer binding T cells can then be measured and localized. In general, any conventional method for diagnostic imaging visualization can be utilized. Usually gamma and positron emitting radioisotopes are used for camera and paramagnetic isotopes for MRI.

The methods described above for direct detection of TCR embedded in lipid bilayers collectively called T cells using MHC multimers also applies to detection of TCR in solution and detection of TCR attached to or in a solid medium. Though detection of individual TCRs may not be possible when TCR is in solution.

Indirect detection of TCR Indirect detection of TCR is primarily usefull for detection of TCRs embedded in lipid bilayer, preferably natural occurring T cells, T cell hybridomas or transfected T cells. In indirect detection, the number or activity of T cells are measured, by detection of events that are the result of TCR-MHC-peptide complex interaction. Interaction between MHC multimer and T cell may stimulate the T cell resulting in activation of T cells, in cell division and proliferation of T cell populations or alternatively result in inactivation of T cells. All these mechanism can be measured using various detection methods.

Indirect detection of T cells by measurement of activation. MHC multimers, e.g. antigen presenting cells, can stimulate T cells resulting in activation of the stimulated T cells. Activation of T cell can be detected by measurement of secretion of specific soluble factor from the stimulated T cell, e.g. secretion of cytokines like INFγ and IL2. Stimulation of T cells can also be detected by measurement of changes in expression of specific surface receptors, or by measurement of T cell effector functions.

Measurement of activation of T cells involves the following steps:

a) To a sample of T cells, preferably a suspension of cells, is added MHC multimer to induce either secretion of soluble factor, up- or down-regulation of surface receptor or other changes in the T cell.

Alternatively, a sample of T cells containing antigen presenting cells is added antigenic peptide or protein/protein fragments that can be processed into antigenic peptides by the antigen presenting cell and that are able to bind MHC I or MHC Il molecules expressed by the antigen presenting cells thereby generating a cell based MHC multimer in the sample. Several different peptides and proteins be added to the sample. The peptide-loaded antigen presenting cells can then stimulate specific T cells, and thereby induce the secretion of soluble factor, up- or down-regulation of surface receptors, or mediate other changes in the T cell, e.g. enhancing effector functions. --O

Optionally a second soluble factor, e.g. cytokine and/or growth factor(s) may be added to facilitate continued activation and expansion of T cell population, b) Detect the presence of soluble factor, the presence/ absence of surface receptor or detect effector function c) Correlate the measured result with presence of T cells. The measured signal/response indicate the presence of specific T cells that have been stimulated with particular MHC multimer.

The signal/response of a T lymphocyte population is a measure of the overall response. The frequency of specific T cells able to respond to a given MHC multimer can be determined by including a limiting-dilution culture in the assay also called a Limiting dilution assay. The limiting-dilution culture method involves the following steps: a) Sample of T cells in suspension are plated into culture wells at increasing dilutions b) MHC multimers are added to stimulate specific T cells . Alternatively antigen presenting cells are provided in the sample and then antigenic peptide I added to the sample as descrinbed above. Optionally growth factors, cytokines or other factors helping T cells to proliferate are added. c) Cells are allowed to grow and proliferate (V2- several days). Each well that initially contained a specific T cell will make a response to the MHC multimer and divide. d) Wells are tested for a specific response e.g. secretion of soluble factors, cell proliferation, cytotoxicity or other effector function. The assay is replicated with different numbers of T cells in the sample, and each well that originally contained a specific T cell will make a response to the MHC multimer. The frequency of specific T cells in the sample equals the reciprocal of the number of cells added to each well when 37% of the wells are negative, because due to Poisson distrubtion each well then on average contained one specific T cell at the beginning of the culture.

In the following various methods to measure secretion of specific soluble factor, expression of surface receptors, effector functions or proliferation is described.

Indirect detection of T cells by measurement of secretion of soluble factors. Indirect detection of T cells by measurement of extracellular secreted soluble factors. Secreted soluble factors can be measured directly in fluid suspension, captured by immobilization on solid support and then detected or an effect of the secreted soluble factor can be detected. o Indirect detection of T cells by measurement of extracellular secreted soluble factor directly in fluid sample.

A sample of T cells are added MHC multimer or antigenic peptide as described above to induce secretion of soluble factors from antigen-specific T cells. The secreted soluble factors can be measured directly in the supernatant using e.g. mass spectrometry.

o Indirect detection of T cells by capture of extracellular secreted soluble factor on solid support. A sample of T cells are added MHC multimer or antigenic peptide as described above to induce secretion of soluble factors from antigen-specific T cells. Secreted soluble factors in the supernatant are then immobilized on a solid support either directly or through a linker as described for immobilization of T cells elsewhere herein. Then immobilized soluble factors can be detected using labeled marker molecules.

Soluble factors secreted from individual T cells can be detected by capturing of the secreted soluble factors locally by marker molecules, e.g antibodies specific for the soluble factor. Soluble factor recognising marker molecules are then immobilised on a solid support together with T cells and soluble factors secreted by individual T cells are thereby captured in the proximity of each T cell. Bound soluble factor can be measured using labelled marker molecule specific for the captured soluble factor. The number of T cells that has given rise to labelled spots on solid support can then be enumerated and these spots indicate the presence of specific T cells that may be stimulated with particular

MHC multimer.

Soluble factors secreted from a population of T cells are detected by capture and detection of soluble factor secreted from the entire population of specific T cells. In this case soluble factor do not have to be captured locally close to each T cell but the secreted soluble factors my be captured and detected in the same well as where the T cells are or transferred to another solid support with marker molecules for capture and detection e.g. beads or wells of ELISA plate.

o Indirect detection of T cells immobilized to solid support in a defined pattern.

Different MHC multimers og MHC-peptide complexes are immobilized to a support to form a spatial array in a defined pattern, where the position specifies the identity of the MHC multimer/MHC-peptide complex immobilized at this position. Marker molecules able to bind T cell secreted soluble factors are co- spotted together with MHC multimer/MHC-peptide complex. Such marker molecules can e.g. be antibodies specific for cytokines like INFγ or IL-2. The immobilization may be direct or through a linker molecule as described above. Then a suspension of labeled T cells are added or passed over the array of MHC multimers/MHC-peptide complexes and specific T cells will bind to the immobilized MHC multimers/MHC-peptide complexes and upon binding be stimulated to secrete soluble factors e.g. cytokines like INFγ ord IL-2. Soluble factors secreted by individual T cells are then captured in the proximity of each T cell and bound soluble factor can be measured using labelled marker molecule specific for the soluble factor. The number and position of different specific T cells that has given rise to labelled spots on solid support can then be identified and enumerated. In this way T cells bound to defined areas of the support are analyzed, thereby, phenotyping the sample. Each individual T cell is defined by the TCR it expose and depending on these TCRs each entity will bind to different types of MHC multimers/MHC-peptide complexes immobilized at defined positions on the solid support.

o Indirect detection of T cells by measurement of effect of extracellular secreted soluble factor.

Secreted soluble factors can be measured and quantified indirectly by measurement of the effect of the soluble factor on other cell systems. Briefly, a sample of T cells are added MHC multimer or antigenic peptide as described above to induce secretion of soluble factors from antigen-specific T cells. The supernatant containing secreted soluble factor are transferred to another cell system and the effect measured. The soluble factor may induce proliferation, secretion of other soluble factors, expression/downregulation of receptors, or the soluble factor may have cytotoxic effects on these other cells,. All effects can be measured as described elsewhere herein.

Indirect detection of T cells by measurement of intracellular secreted soluble factors Soluble factor production by stimulated T cells can be also be measured intracellular by e.g. flow cytometry. This can be done using block of secretion of soluble factor (e.g. by monensin), permeabilization of cell (by e.g. saponine) followed by immunofluorescent staining. The method involves the following steps: 1 ) Stimulation of T cells by binding specific MHC multimers, e.g. antigen presenting cells loaded with antigenic peptide. An reagent able to block extracellular secretion of cytokine is added, e.g. monensin that interrupt intracellular transport processes leading to accumulation of produced soluble factor, e.g. cytokine in the Golgi complex. During stimulation other soluble factors may be added to the T cell sample during stimulation to enhance activation and/or expansionn This other soluble factor can be cytokine and or growth factors. 2) addition of one or more labelled marker able to detect special surface receptors (e.g. CD8,

CD4, CD3, CD27, CD28, CD2). 3) Fixation of cell membrane using mild fixator followed by permeabilization of cell membrane by. e.g. saponine. 4) Addition of labelled marker specific for the produced soluble factor to be determined, e.g. INFγ, IL-2, IL-4, IL-10. 5) Measurement of labelled cells using a flow cytometer. An alternative to this procedure is to trap secreted soluble factors on the surface of the secreting T cell as described by Manz, R. et al., Proc. Natl. Acad. Sci. USA 92:1921 (1995).

Indirect detection of T cells by measurement of expression of receptors Activation of T cells can be detected by measurement of expression and/or down regulation of specific surface receptors. The method includes the following steps. A sample of T cells are added MHC multimer or antigenic peptide as described above to induce expression or downregulation of specific surface receptors on antigen-specific T cells.These receptors include but are not limited to CD28, CD27, CCR7, CD45RO, CD45RA, IL2-receptor, CD62L, CCR5. Their expression level can be detected by addition of labelled marker specific for the desired receptor and then measure the amount of label using flow cytometry, microscopy, immobilization of activated T cell on solid support or any other method like those decribed for direct detection of TCR in lipid bilayer. Indirect detection of T cells by measurement of effector function Activation of T cells can be detected indirectly by measurement of effector functions. A sample of T cells are added MHC multimer or antigenic peptide as described above to induce the T cell to be able to do effector function. The effector function is then measured. E.g. activation of antigen-specific CD8 positive T cells can be measured in a cytotoxicity assay.

Indirect detection of T cells by measurement of proliferation

T cells can be stimulated to proliferate upon binding specific MHC multimers. Proliferation of T cells can be measured several ways including but not limited to: o Detection of mRNA

Proliferation of T cells can be detected by measurement of mRNA inside cell. Cell division and proliferation requires production of new protein in each cell which as an initial step requires production of mRNA encoding the proteins to be synthesized.

A sample of T cells are added MHC multimer or antigenic peptide as described above to induce proliferation of antigen-specific T cells. Detection of levels of mRNA inside the proliferating T cells can be done by quantitative PCR and indirectly measure activation of a T cell population as a result of interaction with MHC multimer. An example is measurement of cytokine mRNA by in sity hybridization.

o Detection of incorporation of thymidine

The proliferative capacity of T cells in response to stimulation by MHC multimer can be determined by a radioactive assay based on incorporation of [³H]thymidine

([³H]TdR) into newly generated DNA followed by measurement of radioactive signal.

o Detection of incorporation of BrdU T cell proliferation can also be detected by of incorporation of bromo-2'- deoxyuridine (BrdU) followed by measurement of incorporated BrdU using a labeled anti-BrdU antibody in an ELISA based analysis.

Viability of cells may be measured by measurement ATP in a cell culture. Indirect detection of T cells by measurement of inactivation

Not all MHC multimers will lead to activation of the T cells they bind. Under certain circumstances some MHC multimers may rather inactivate the T cells they bind to.

Indirect detection of T cells by measurement of effect of blockade of TCR

Inactivation of T cells by MHC multimers may be measured be measuring the effect of blocking TCR on antigen-specific T cells. MHC multimers, e.g. MHC-peptide complexes coupled to IgG scaffold can block the TCR of an antigen-specific T cell by binding the TCR, thereby prevent the blocked T cell receptor interacting with e.g. antigen presenting cells. Blockade of TCRs of a T cell can be detected in any of the above described medthods for detection of TCR by addition of an unlabeled blocking MHC multimer together with the labelled MHC multimer and then measuring the effect of the blockade on the readout.

Indirect detection of T cells by measurement of induction of apoptosis

Inactivation of T cells by MHC multimers may be measured be measuring apoptosis of the antigen-specific T cell. Binding of some MHC multimers to specific T cells may lead to induction of apoptosis. Inactivation of T cells by binding MHC multimer may therefore be detected by measuring apoptosis in the T cell population. Methods to measure apoptosis in T cells include but are not limited to measurement of the following:

• DNA fragmentation

• Alterations in membrane asymmetry (phosphatidylserine translocation)

• Activation of apoptotic caspases

• Release of cytochrome C and AIF from mitochondria into the cytoplasm

Positive control experiments for the use of MHC multimers in flow cytometry and related techniques

When performing flow cytometry experiments, or when using similar technologies, it is important to include appropriate positive and negative controls. In addition to establishing proper conditions for the experiments, positive and negative control reagents can also be used to evaluate the quality (e.g. specificity and affinity) and stability (e.g. shelf life) of produced MHC multimers.

The quality and stability of a given MHC multimer can be tested in a number of different ways, including: • Measurement of specific MHC multimer binding to beads, other types of solid support, or micelles and liposomes, to which TCR's have been immobilized. Other kinds of molecules that recognize specifically the MHC-peptide complex can be immobilized and used as well. Depending on the nature of the solid support or membrane structure to which the TCR is immobilized, the TCR can be full-length (i.e. comprise the intracellular- and intra-membrane domains), or can be truncated (e.g. only comprise the extracellular domains). Likewise, the TCR can be recombinant, and can be chemically or enzymatically modified. • Measurement of MHC multimer binding to beads, other types of solid support, or micelles and liposomes, to which aptamers, antibodies or other kinds of molecules that recognize correctly folded MHC-peptide complexes have been immobilized.

• Measurement of specific MHC multimer binding to specific cell lines (e.g. T-cell lines) displaying MHC multimer-binding molecules, e.g. displaying TCRs with appropriate specificity and affinity for the MHC multimer in question.

• Measurement of specific MHC multimer binding to cells in blood samples, preparations of purified lymphocytes (HPBMCs), or other bodily fluids that contain cells carrying receptor molecules specific for the MHC multimer in question.

• Measurement of specific MHC multimer binding to soluble TCRs, aptamers, antibodies, or other soluble MHC-peptide complex-binding molecules, by density-gradient centrifugation (e.g. in CsCI) or by size exclusion chromatography, PAGE or other type of chromatographic method. Measurement of specific MHC binding to TCRs, aptamers, antibodies, streptavidin, or other MHC-peptide complex-binding molecules immobilized on a solid surface (e.g. a microtiter plate). The degree of MHC multimer binding can be visualized with a secondary component that binds the MHC multimer, e.g. a biotinylated fluorophore in cases where the MHC multimer contains streptavidin proteins, not fully loaded with biotin. Alternatively, the secondary component is unlabelled, and a labelled second component-specific compound is employed (e.g. EnVision System, Dako) for visualization. This solid surface can be beads, immunotubes, microtiterplates act. The principle for purification are basically the same I.e. T cells are added to the solid with immobilized MHC'mer, non-binding T cells are washed away and MHC-peptide specific T cells can be retrieved by elution with mild acid or a competitive binding reagent. • Measurement of specific MHC multimer binding to TCRs, aptamers, antibodies, streptavidin, or other MHC-peptide complex-binding molecules immobilized on a solid surface (e.g. a microtiter plate) visualized with a secondary component specific to MHC multimer (e.g. TCRs, aptamers, antibodies, streptavidin, or other MHC-peptide binding complex-binding molecules). Alternatively the secondary receptor is unlabelled, and a labelled second receptor-specific compound is employed (e.g. EnVision System, Dako) before visualization.

In the above mentioned approaches, positive control reagents include MHC multimers comprising correctly folded MHC, complexed with an appropriate peptide that allows the MHC multimer to interact specifically and efficiently with its cognate TCR. Negative control reagents include empty MHC multimers, or correctly folded MHC multimers complexed with so-called nonsense peptides that support a correct conformation of the MHC-peptide complex, but that do not efficiently bind TCRs through the peptide- binding site of the MHC complex.

Negative control reagents and negative control experiments for the use of MHC multimers in flow cytometry and related techniques Experiments with MHC multimers require a negative control in order to determine background staining with MHC multimer. Background staining can be due to unwanted binding of any of the individual components of the MHC multimer, e.g., MHC complex or individual components of the MHC complex, multimerization domain or label molecules. The unwanted binding can be to any surface or intracellular protein or other cellular structure of any cell in the test sample, e.g. undesired binding to B cells, NK cells or T cells. Unwanted binding to certain cells or certain components on cells can normally be corrected for during the analysis, by staining with antibodies that bind to unique surface markers of these specific cells, and thus identifies these as false positives, or alternatively, that bind to other components of the target cells, and thus identifies these cells as true positives. A negative control reagent can be used in any experiment involving MHC multimers, e.g. flow cytometry analysis, other cytometric methods, immunohistochemistry (IHC) and ELISA. Negative control reagents include the following: • MHC complexes or MHC multimers comprising MHC complexes carrying nonsense peptides. A nonsense peptide is here to be understood as a peptide that binds the MHC protein efficiently, but that does not support binding of the resultant MHC- peptide complex to the desired TCR. An example nonsense peptide is a peptide with an amino acid sequence different from the linear sequence of any peptide derived from any known protein. When choosing an appropriate nonsense peptide the following points are taken into consideration. The peptide should ideally have appropriate amino acids at relevant positions that can anchor the peptide to the peptide-binding groove of the MHC. The remaining amino acids should ideally be chosen in such a way that possible binding to TCR (through interactions with the peptide or peptide-binding site of MHC) are minimized. The peptide should ideally be soluble in water to make proper folding with MHC alpha chain and β2m possible in aqueous buffer. The length of the peptide should ideally match the type and allele of MHC complex. The final peptide sequence should ideally be taken through a blast search or similar analysis, to ensure that it is not identical with any peptide sequence found in any known naturally occurring proteins.

• MHC complexes or MHC multimers comprising MHC complexes carrying a chemically modified peptide in the peptide-binding groove. The modification should ideally allow proper conformation of the MHC-peptide structure, yet should not allow efficient interaction of the peptide or peptide-binding site of MHC with the

TCR.

• MHC complexes or MHC multimers comprising MHC complexes carrying a naturally occurring peptide different from the peptide used for analysis of specific T cells in the sample. When choosing the appropriate natural peptide the following should be taken into consideration. The peptide in complex with the MHC protein should ideally not be likely to bind a TCR of any T cell in the sample with such an affinity that it can be detected with the applied analysis method. The peptide should ideally be soluble in water to make proper folding with MHC alpha chain and β2m possible in aqueous buffer. The length of the peptide should match the type and allele of MHC complex.

• Empty MHC complexes or MHC multimers comprising empty MHC complexes, meaning any correctly folded MHC complex without a peptide in the peptide- binding groove. O

MHC heavy chain or MHC multimers comprising MHC heavy chain, where MHC heavy chain should be understood as full-length MHC I or MHC Il heavy chain or any truncated version of MHC I or MHC Il heavy chain. The MHC heavy chains can be either folded or unfolded. Of special interest is MHC I alpha chains containing the oc3 domain that binds CD8 molecules on cytotoxic T cells. Another embodiment of special interest is MHC Il β chains containing the β2 domain that binds CD4 on the surface of helper T cells.

• Beta2microglobulin or subunits of beta2microglobulin, or MHC multimers comprising Beta2microglobulin or subunits of beta2microglobulin, folded or unfolded.

• MHC-like complexes or MHC multimers comprising MHC-like complexes, folded or unfolded. An example could be CD1 molecules that are able to bind peptides in a peptide-binding groove that can be recognized by T cells (Russano et al. (2007). CD1 -restricted recognition of exogenous and self-lipid antigens by duodenal gammadelta÷ T lymphocytes. J Immunol. 178(6):3620-6 )

• Multimerization domains without MHC or MHC-like molecules, e.g. dextran, streptavidin, IgG, coiled-coil-domain liposomes.

• Labels, e.g. FITC, PE, APC, pacific blue, cascade yellow, or any other label listed elsewhere herein.

Negative controls 1 -4 can provide information about potentially undesired binding of the MHC multimer, through interaction of a surface of the MHC-peptide complex different from the peptide-binding groove and its surroundings. Negative control 5 and 6 can provide information about binding through interactions through the MHC I or MHC Il proteins (in the absence of peptide). Negative control 7 can provide information about binding through surfaces of the MHC complex that is not unique to the MHC complex. Negative controls 8 and 9 provide information about pontential undesired interactions between non-MHC-peptide complex components of the MHC multimer and cell constituents. o

Minimization of undesired binding of the MHC multimer

Identification of MHC-peptide specific T cells can give rise to background signals due to unwanted binding to cells that do not carry TCRs. This undesired binding can result from binding to cells or other material, by various components of the MHC multimer, e.g. the dextran in a MHC dextramer construct, the labelling molecule (e.g. FITC), or surface regions of the MHC-peptide complex that do not include the peptide and the peptide-binding cleft.

MHC-peptide complexes bind to specific T cells through interaction with at least two receptors in the cell membrane of the T-cell. These two receptors are the T-cell receptor (TCR) and CD8 for MHC l-peptide complexes and TCR and CD4 receptor protein for MHC I l-peptide complexes. Therefore, a particularly interesting example of undesired binding of a MHC multimer is its binding to the CD8 or CD4 molecules of T cells that do not carry a TCR specific for the actual MHC-peptide complex. The interaction of CD8 or CD4 molecules with the MHC is not very strong; however, because of the avidity gained from the binding of several MHC complexes of a MHC multimer, the interaction between the MHC multimer and several CD8 or CD4 receptors potentially can result in undesired but efficient binding of the MHC multimer to these T cells. In an analytical experiment this would give rise to an unwanted background signal; in a cell sorting experiment undesired cells might become isolated. Other particular interesting examples of undesired binding is binding to lymphoid cells different from T cells, e.g. NK-cells, B-cells, monocytes, dendritic cells, and granulocytes like eosinophils, neutrophils and basophiles.

Apart from the MHC complex, other components in the MHC multimer can give rise to unspecific binding. Of special interest are the multimerization domain, multimerization domain molecules, and labelling molecules.

One way to overcome the problem with unwanted binding is to include negative controls in the experiment and subtract this signal from signals derived from the analyzed sample, as described elsewhere in the invention.

Alternatively, unwanted binding could be minimized or eliminated during the experiment. Methods to minimize or eliminate background signals include:

• Mutations in areas of the MHC complex responsible for binding to unwanted cells can be introduced. Mutations here mean substitution, insertion, or deletion of natural or non-natural amino acids. Sub-domains in the MHC complex can be responsible for unwanted binding of the MHC multimer to cells without a TCR specific for the MHC-peptide complex contained in the MHC multimer. One example of special interest is a small region in the oc3-domain of the α-chain of MHC I molecules that is responsible for binding to CD8 on all cytotoxic T cells.

Mutations in this area can alter or completely abolish the interaction between CD8 on cytotoxic T cells and MHC multimer (Neveu et al. (2006) lnt Immunol. 18, 1 139- 45). Similarly a sub domain in the β2 domain of the β-chain of MHC Il molecules is responsible for binding CD4 molecules on all CD4 positive T cells. Mutations in this sub domain can alter or completely abolish the interaction between MHC Il and

CD4.

Another embodiment is to mutate other areas of MHC I /MHC Il complexes that are involved in interactions with T cell surface receptors different from TCR, CD8 and CD4, or that bind surface receptors on B cells, NK cells, Eosiniophils, Neutrophils, Basophiles, Dendritic cells or monocytes.

• Chemical alterations in areas of the MHC complex responsible for binding to unwanted cells can be employed in order to minimize unwanted binding of MHC multimer to irrelevant cells. Chemical alteration here means any chemical modification of one or more amino acids. Regions in MHC complexes that are of special interest are as mentioned above the α3 domain of the α-chain in MHC I molecules and β2 domains in the β-chain of MHC Il molecules. Other regions in MHC I /MHC Il molecules that can be chemically modified to decrease the extent of undesired binding are regions involved in interaction with T cell surface receptors different from TCR, CD8 and CD4, or that bind surface receptors on B cells, NK cells, Eosiniophils, Neutrophils, Basophiles, Dendritic cells or monocytes.

• Another method to minimize undesired binding involves the addition of one or more components of a MHC multimer, predicted to be responsible for the unwanted binding. The added component is not labeled, or carries a label different from the label of the MHC multimer used for analysis. Of special interest is addition of MHC multimers that contain nonsense peptides, i.e. peptides that interact efficiently with the MHC protein, but that expectably do not support specific binding of the MHC multimer to the TCR in question. Another example of interest is addition of soluble MHC complexes not coupled to a multimerization domain, and with or without peptide bound in the peptide binding cleft. In another embodiment, individual components of the MHC complex can be added to the sample, e.g. I α-chain or subunits of MHC I α-chain either folded or unfolded, beta2microglobulin or subunits thereof either folded or unfolded, α/β-chain of MHC Il or subunits thereof either folded or unfolded. Any of the above mentioned individual components can also be attached to a multimerization domain identical or different from the one used in the MHC multimer employed in the analysis.

Of special interest is also addition of multimerization domain similar or identical to the multimerization domain used in the MHC multimer or individual components of the multimerization domain.

• Reagents able to identify specific cell types either by selection or exclusion can be included in the analysis to help identify the population of T cells of interest, and in this way deselect the signal arising from binding of the MHC multimer to undesired cells. Of special interest is the use of appropriate gating reagents in flow cytometry experiments. Thus, fluorescent antibodies directed against specific surface markers can be used for identification of specific subpopulations of cells, and in this way help to deselect signals resulting from MHC multimers binding to undesired cells. Gating reagents of special interest that helps identify the subset of T cells of interest when using MHC I multimers are reagents binding to CD3 and CD8 identifying all cytotoxic T cells. These reagents are preferably antibodies but can be any labeled molecule capable of binding CD3 or CD8. Gating reagents directed against CD3 and CD8 are preferably used together. As they stain overlapping cell populations they are preferably labeled with distinct fluorochromes. However, they can also be used individually in separate samples. In experiments with MHC Il multimers reagents binding to CD3 and CD4 identifying T helper cells can be used. These reagents are preferably antibodies but can be any labeled molecule capable of binding CD3 or CD4. Gating reagents directed against CD3 and CD4 are preferable used together. As they stain overlapping cell populations they are preferably labeled with distinct fluorochromes. However, they can also be used individually in separate samples.

Other gating reagents of special interest in experiments with any MHC multimer, are reagents binding to the cell surface markers CD2, CD27, CD28, CD45RA, CD45RO, CD62L and CCR7. These surface markers are unique to T cells in various differentiation states. Co staining with either of these reagents or combinations thereof together with MHC multimers helps to select MHC multimer binding T cells expressing a correct TCR. These reagents can also be combined with reagents directed against CD3, CD4 and/or CD8. Another flow cytometric method of special interest to remove signals from MHC multimer stained cells not expressing the specific TCR, is to introduce an exclusion gate. Antibodies or other reagents specific for surface markers unique to the unwanted cells are labeled with a fluorochrome and added to the test sample together with the MHC multimer. The number of antibodies or surface marker specific reagents are not limited to one but can be two, three, four, five, six, seven, eight, nine, ten or more individual reagents recognizing different surface markers, all of which are unique to the unwanted cells. During or after collection of data all events representing cells labeled with these antibodies are dumped in the same gate and removed from the dataset. This is possible because all the antibodies/reagents that bind to the wrong cells are labeled with the same fluorochrome.

Reagents of special interest that exclude irrelevant cells include reagents against CD45 expressed on red blood cells, CD19 expressed on B cells, CD56 expressed on NK cells, CD4 expressed on T helper cells and CD8 expressed on cytotoxic T cells, CD14 expressed on monocytes and CD15 expressed on granulocytes and monocytes.

Vaccine treatment

For the purpose of making vaccines it can be desirable to employ MHC multimers that comprise a polymer such as dextran, or that are cell-based (e.g. specialized dendritic cells such as described by Banchereau and Palucka, Nature Reviews, Immunology, 2005, vol. 5, p. 296-306).

D Preventive vaccination leading to prophylaxis/sterile immunity by inducing memory in the immune system may be obtained by immunizing/vaccinating an individual or animal with MHC alone, or with MHC in combination with other molecules as mentioned elsewhere in the patent. o Vaccine antigens can be administered alone o Vaccine can be administered in combination with adjuvant(s).

D Adjuvant can be mixed with vaccine component or administered alone, simultaneously or in any order. D Adjuvant can be administered by the same route as the other vaccine components o Vaccine administered more than once may change composition from 1 ^st administration to the 2^nd, 3^rd, etc. o Vaccine administered more than once can be administered by alternating routes o Vaccine components can be administered alone or in combinations by the same route or by alternating/mixed routes o Vaccine can be administered by the following routes D Cutaneously

D Subcutaneously (SC) D Intramuscular (IM) D Intravenous (IV) D Per-oral (PO) D Inter peritoneally

D Pulmonally D Vaginally D Rectal Iy D Therapeutic vaccination i.e. vaccination "teaching" the immune system to fight an existing infection or disease, may be obtained by immunizing/vaccinating an individual or animal with MHC alone, or with MHC in combination with other molecules as mentioned elsewhere in the patent. o Vaccine antigens can be administered alone o Vaccine can be administered in combination with adjuvant(s). D Adjuvant can be mixed with vaccine component or administered alone, simultaneously or in any order. D Adjuvant can be administered by the same route as the other vaccine components o Vaccine administered more than once may change composition from 1 ^st administration to the 2^nd, 3^rd, etc. o Vaccine administered more than once can be administered by alternating routes o Vaccine components can be administered alone or in combinations by the same route or by alternating/mixed routes o Vaccine can be administered by the following routes

D Cutaneously

D Subcutaneously (SC)

D Intramuscular (IM)

D Intravenous (IV)

D Per-oral (PO)

D Inter peritoneally

D Pulmonally

D Vaginally

D Rectal Iy

Therapeutic treatment

D Theraoeutic treatment includes the i any molecular combination mentioned elsewhere in the patent application for the purpose of treating a disease in any state. Treatment may be in the form of o Per-orally intake

D Pills D Capsules o Injections

D Systemic D Local

D o Jet-infusion (micro-drops, micro-spheres, micro-beads) through skin o Drinking solution, suspension or gel o Inhalation o Nose-drops o Eye-drops o Ear-drops o Skin application as ointment, gel or creme o Vaginal application as ointment, gel, creme or washing o Gastro-lntestinal flushing o Rectal washings or by use of suppositories

D Treatment can be performed as o Single intake, injection, application, washing o Multiple intake, injection, application, washing D On single day basis D Over prolonged time as days, month, years D

D Treatment dose and regimen can be modified during the course

Personalized medicine takes advantage of the large diversity of peptide epitopes that may be generated from a given antigen.

The immune system is very complex. Each individual has a very large repertoire of specific T cells (on the order of 10⁶-10⁹ different T cell specificities), which again is only a small subset of the total T cell repertoire of a population of individuals. It is estimated that the Caucasian population represents a T cell diversity of 10¹⁰-10¹². MHC allele diversity combined with large variation among individuals' proteolytic metabolism further enhances the variation among different individuals' immune responses. As a result, each individual has its own characteristic immune response profile.

This is important when designing a MHC multimer-based immune monitoring reagent or immunotherapeutic agent. If an agent is sought that should be as generally applicable as possible, one should try to identify peptide epitopes and MHC alleles that are common for the majority of individuals of a population. As described elsewhere in this application, such peptide epitopes can be identified through computerized search algorithms developed for that same purpose, and may be further strengthened by experimental testing of a large set of individuals.

This approach will be advantageous in many cases, but because of the variability among immune responses of different individuals, is likely to be inefficient or inactive in certain individuals, because of these individuals' non-average profile. In these latter cases one may have to turn to personalized medicine. In the case of immune τ"

monitoring and immunotherapy, this may involve testing a large number of different epitopes from a given antigen, in order to find peptide epitopes that may provide MHC multimers with efficiency for a given individual.

Thus, personalized medicine takes advantage of the wealth of peptide epitopes that may be generated from a given antigen. A large number of the e.g. 8-, 9-, 10-, and 1 1 - mer epitopes that may be generated from a given antigen to be included in a class 1 MHC multimer reagent, for use in immune monitoring or immunotherapy, are therefore of relevance in personalized medicine. Only in the case where one wants to generate a therapeutic agent or diagnostic reagent that is applicable to the majority of individuals of a population can the large majority of epitope sequences be said to be irrelevant, and only those identified by computerized search algorithms and experimental testing be said to be of value. For the odd individual with the odd immune response these disregarded peptide epitopes may be the epitopes that provide an efficient diagnostic reagent or cures that individual from a deadly disease.

Antigenic peptides

The present invention relates in one embodiment to antigenic peptides derived from CMV antigens. The one or more antigenic peptides can in one embodiment comprise one or more fragments from one or more CMV antigens capable of interacting with one or more MHC class 1 molecules. The one or more antigenic peptides can in another embodiment comprise one or more fragments from one or more CMV antigens capable of interacting with one or more MHC class 2 molecules.

The antigenic peptides can be generated from any CMV antigen such as the CMV antigens listed in Table 6.

Table 6: CMV derived antigens

MHC Class I and MHC Class Il molecules have different structures, as described above, and therefore have different restrictions on the size of the peptide which may be accommodated. In general, MHC Class I molecules will accommodate peptides of from about 8 amino acids in length to about 1 1 amino acids. MHC Class Il molecules will in general accommodate peptides of from about 13 amino acids in length to about 16 amino acids. Peptides derived from the sequences shown in Table 6, for use preferably with MHC Class I or ll-based multimers are shown in Table 9. The antigenic peptides can in one embodiment be generated by computational prediction using NetMHC (www.cbs.dtu.dk/services/NetMHC/) or by selected of specific 8, 9, 10, 1 1 , 13, 14, 15 or 16 amino acid sequences.

The present invention relates to one or more MHC multimers and/or one or more MHC complexes comprising one or more antigenic peptides such as the antigenic peptides listed in Table 7, Table 8 and/or Table 9 (SEQ ID NO 1 to SEQ ID NO 9697) and/or the antigenic peptides characterized by item 1 to 735 herein below.

The one or more antigenic peptides can in one embodiment comprise or consist of a fragment of one or more antigenic peptides listed in Table 7, Table 8 and/or Table 9 (SEQ ID NO 1 to SEQ ID NO 9697) and/or the antigenic peptides characterized by item 1 to 735 herein below, such as a fragment consisting of 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, or 15 amino acids.

In another embodiment the antigenic peptide listed in Table 7, Table 8 and/or Table 9 (SEQ ID NO 1 to SEQ ID NO 9697) and/or the antigenic peptides characterized by item 1 to 735 herein below can be part of a larger peptide/protein, wherein the larger peptide/protein may be of a total length of 17, such as 18, for example 19, such as 20, for example 21 , such as 22, for example 23, such as 24, for example 25, such as 26, for example 27, such as 28, for example 29, such as 30, for example 31 , such as 32, for example 33, such as 34, for example 35, such as 36, for example 37, such as 38, for example 39, such as 40 amino acids, wherein 8 to 16 of said amino acids are defined in the items below. In another embodiment, the larger protein may be of a total length of between 20 to 30, such as 30-40, for example 40-50, such as 50-60, for example 60-70, such as 70-80, for example 80-90, such as 90-100, for example 100- 150, such as 150-200, for example 200-250, such as 250-300, for example 300-500, such as 500-1000, for example 1000-2000, such as 2000-3000, for example 3000- 4000, such as 4000-5000, for example 5000-10,000, such as 10,000-20,000, for example 20,000-30,000, such as 30,000-40,000, for example 40,000-50,000, such as 50,000-75,000, for example 75,000-100,000, such as 100,000-250,000, for example 250,000-, 500, 000, such as 500,000-1 ,000,000 amino acids. In one embodiment the antigenic peptides listed in Table 7, Table 8 and/or Table 9 (SEQ ID NO 1 to SEQ ID NO 9697) are modified by one or more type(s) of post- translational modifications such as one or more of the post-translational modifications listed in the items (item 1 to 735) herein below. The same or different types of post- translational modification can occur on one or more amino acids in the antigenic peptide such as on 2, 3, 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15 or 16 amino acids.

Table 7: Prediction of cancer antigen BcIX(L) specific MHC class 1 , 8- , 9- ,10- ,11-mer peptide binders.

Prediction of cancer antigen BcIX(L) specific MHC classi , 8- , 9- ,10- ,1 1 -mer peptide binders for 24 MHC class 1 alleles using the http://www.cbs.dtu.dk/services/NetMHC/ database. The MHC class 1 molecules for which no binders were found are not listed. pos peptide logscore affinity (nM) Bind Level Protein Name Allele

8—mers

57 HLADSPAV 0.691 28 SB Sequence A0201

213 FLTGMTVA 0.687 29 SB Sequence A0201

166 AAWMATYL 0.477 285 WB Sequence A0201

160 VLVSRIAA 0.463 333 WB Sequence A0201

119 YQSFEQW 0.436 448 WB Sequence A0201

147 GALCVESV 0.431 472 WB Sequence A0201

223 VLLGSLFS 0.427 494 WB Sequence A0201

213 FLTGMTVA 0.777 11 SB Sequence A0202

57 HLADSPAV 0.771 11 SB Sequence A0202

119 YQSFEQW 0.590 84 WB Sequence A0202

218 TVAGWLL 0.565 110 WB Sequence A0202

11 FLSYKLSQ 0.545 137 WB Sequence A0202

82 MAAVKQAL 0.512 195 WB Sequence A0202

73 SLDAREVI 0.475 294 WB Sequence A0202

192 ELYGNNAA 0.444 410 WB Sequence A0202

217 MTVAGWL 0.440 425 WB Sequence A0202

160 VLVSRIAA 0.434 454 WB Sequence A0202

1 SQSNRELV 0.434 457 WB Sequence A0202

213 FLTGMTVA 0.852 SB Sequence A0203

57 HLADSPAV 0.831 SB Sequence A0203

160 VLVSRIAA 0.642 48 SB Sequence A0203

158 MQVLVSRI 0.602 74 WB Sequence A0203

11 FLSYKLSQ 0.582 92 WB Sequence A0203

133 GVNWGRIV 0.581 92 WB Sequence A0203

216 GMTVAGW 0.579 94 WB Sequence A0203

119 YQSFEQW 0.578 96 WB Sequence A0203

164 RIAAWMAT 0.573 101 WB Sequence A0203

78 EVIPMAAV 0.486 261 WB Sequence A0203

1 SQSNRELV 0.481 274 WB Sequence A0203

217 MTVAGWL 0.467 318 WB Sequence A0203

147 GALCVESV 0.464 328 WB Sequence A0203

221 GWLLGSL 0.443 412 WB Sequence A0203

218 TVAGWLL 0.440 429 WB Sequence A0203

57 HLADSPAV 0.555 122 WB Sequence A0204

153 SVDKEMQV 0.431 469 WB Sequence A0204

57 HLADSPAV 0.780 10 SB Sequence A0206

158 MQVLVSRI 0.733 18 SB Sequence A0206

213 FLTGMTVA 0.682 31 SB Sequence A0206

1 SQSNRELV 0.677 32 SB Sequence A0206

119 YQSFEQW 0.677 33 SB Sequence A0206

138 RIVAFFSF 0.653 42 SB Sequence A0206

164 RIAAWMAT 0.575 99 WB Sequence A0206

147 GALCVESV 0.568 106 WB Sequence A0206 166 AAWMATYL 0.567 108 WB Sequence A0206

217 MTVAGWL 0 .563 112 WB Sequence A0206

160 VLVSRIAA 0 .517 185 WB Sequence A0206

42 SEMETPSA 0 .514 191 WB Sequence A0206

78 EVIPMAAV 0 .496 233 WB Sequence A0206

153 SVDKEMQV o .493 240 WB Sequence A0206

57 HLADSPAV o 955 1 SB Sequence A0211

153 SVDKEMQV o .898 3 SB Sequence A0211

213 FLTGMTVA 0 .893 SB Sequence A0211

73 SLDAREVI 0 .877 SB Sequence A0211

192 ELYGNNAA 0 .834 6 SB Sequence A0211

218 TVAGWLL 0 .797 g SB Sequence A0211

172 YLNDHLEP 0 .751 14 SB Sequence A0211

78 EVIPMAAV o .739 16 SB Sequence A0211

216 GMTVAGW o .718 21 SB Sequence A0211

160 VLVSRIAA o .684 30 SB Sequence A0211

223 VLLGSLFS o .683 30 SB Sequence A0211

133 GVNWGRIV 0 .668 36 SB Sequence A0211

212 WFLTGMTV 0 .668 36 SB Sequence A0211

144 SFGGALCV 0 .591 83 WB Sequence A0211

72 SSLDAREV 0 .590 84 WB Sequence A0211

106 DLTSQLHI o .564 111 WB Sequence A0211

119 YQSFEQW o .545 136 WB Sequence A0211

81 PMAAVKQA o .532 158 WB Sequence A0211

11 FLSYKLSQ o .511 198 WB Sequence A0211

166 AAWMATYL 0 .456 360 WB Sequence A0211

1 SQSNRELV 0 .439 431 WB Sequence A0211

147 GALCVESV 0 .439 434 WB Sequence A0211

57 HLADSPAV 0 .915 2 SB Sequence A0212

192 ELYGNNAA 0 .813 7 SB Sequence A0212

213 FLTGMTVA 0 .801 8 SB Sequence A0212

153 SVDKEMQV 0 .732 18 SB Sequence A0212

73 SLDAREVI 0 .714 22 SB Sequence A0212

160 VLVSRIAA 0 .662 38 SB Sequence A0212

172 YLNDHLEP 0 .662 38 SB Sequence A0212

119 YQSFEQW 0 .586 88 WB Sequence A0212

78 EVIPMAAV 0 .585 88 WB Sequence A0212

223 VLLGSLFS 0 .582 92 WB Sequence A0212

11 FLSYKLSQ 0 .573 101 WB Sequence A0212

212 WFLTGMTV 0 .541 142 WB Sequence A0212

216 GMTVAGW 0 .466 321 WB Sequence A0212

57 HLADSPAV 0 .892 3 SB Sequence A0216

153 SVDKEMQV 0 .817 7 SB Sequence A0216

213 FLTGMTVA 0 .761 13 SB Sequence A0216

192 ELYGNNAA 0 .715 21 SB Sequence A0216

78 EVIPMAAV 0 .666 37 SB Sequence A0216

218 TVAGWLL 0 .657 41 SB Sequence A0216

73 SLDAREVI 0 .640 49 SB Sequence A0216

144 SFGGALCV 0 .630 54 WB Sequence A0216

216 GMTVAGW 0 .613 65 WB Sequence A0216

166 AAWMATYL 0 .603 73 WB Sequence A0216

160 VLVSRIAA 0 .583 91 WB Sequence A0216

212 WFLTGMTV 0 .565 110 WB Sequence A0216

11 FLSYKLSQ 0 .488 255 WB Sequence A0216

106 DLTSQLHI o .487 258 WB Sequence A0216

133 GVNWGRIV o .470 308 WB Sequence A0216

81 PMAAVKQA 0 .469 311 WB Sequence A0216

118 AYQSFEQV 0 .461 342 WB Sequence A0216

223 VLLGSLFS 0 .442 417 WB Sequence A0216

147 GALCVESV 0 .438 436 WB Sequence A0216

57 HLADSPAV o .924 2 SB Sequence A0219

213 FLTGMTVA o .668 36 SB Sequence A0219

153 SVDKEMQV o .597 78 WB Sequence A0219

73 SLDAREVI o .576 98 WB Sequence A0219

218 TVAGWLL 0 .517 185 WB Sequence A0219

192 ELYGNNAA 0 .486 259 WB Sequence A0219

212 WFLTGMTV 0 .458 352 WB Sequence A0219

166 AAWMATYL 0 .455 362 WB Sequence A0219

106 DLTSQLHI o .448 390 WB Sequence A0219

223 VLLGSLFS o .431 471 WB Sequence A0219 LSYKLSQK 0.761 13 SB Sequence A0301

WDFLSYK 0.551 128 WB Sequence A0301

LLGSLFSR 0.487 257 WB Sequence A0301

WDFLSYK 0.751 14 SB Sequence AIlOl

LSYKLSQK 0.721 20 SB Sequence AIlOl

VIPMAAVK 0.509 203 WB Sequence AIlOl

QWNELFR 0.472 302 WB Sequence AIlOl

LWDFLSY 0.457 355 WB Sequence AIlOl

NAAAESRK 0.455 363 WB Sequence AIlOl

NWGRIVAF 0.600 75 WB Sequence A2301

RIVAFFSF 0.466 321 WB Sequence A2301

WLLGSLF 0.461 339 WB Sequence A2301

NWGRIVAF 0.617 62 WB Sequence A2402

AYQSFEQV 0.569 105 WB Sequence A2403

EVIPMAAV 0.598 77 WB Sequence A2601

LWDFLSY 0.541 144 WB Sequence A2601

EVIPMAAV 0.862 4 SB Sequence A2602

LWDFLSY 0.797 9 SB Sequence A2602

HIIPGIAY 0.755 14 SB Sequence A2602

ELRYRRAF 0.589 85 WB Sequence A2602

RIVAFFSF 0.529 164 WB Sequence A2602

HIIPGIAY 0.597 78 WB Sequence A2902

LWDFLSY 0.480 276 WB Sequence A2902

KGQERFNR 0.743 16 SB Sequence A3101

LLGSLFSR 0.697 26 SB Sequence A3101

EMQVLVSR 0.583 90 WB Sequence A3101

HSSSLDAR 0.577 97 WB Sequence A3101

AAVKQALR 0.539 146 WB Sequence A3101

EFELRYRR 0.509 201 WB Sequence A3101

QWNELFR 0.453 369 WB Sequence A3101

LSYKLSQK 0.447 397 WB Sequence A3101

EFELRYRR 0.823 6 SB Sequence A3301

EMQVLVSR 0.738 16 SB Sequence A3301

DEFELRYR 0.650 43 SB Sequence A3301

ESRKGQER 0.606 71 WB Sequence A3301

LLGSLFSR 0.538 148 WB Sequence A3301

HSSSLDAR 0.463 332 WB Sequence A3301

QWNELFR 0.803 8 SB Sequence A6801

HSSSLDAR 0.775 11 SB Sequence A6801

NAAAESRK 0.681 31 SB Sequence A6801

LSYKLSQK 0.647 45 SB Sequence A6801

EMQVLVSR 0.599 76 WB Sequence A6801

NNAAAESR 0.585 88 WB Sequence A6801

AAVKQALR 0.535 153 WB Sequence A6801

ESRKGQER 0.532 158 WB Sequence A6801

IAAWMAIY 0.532 158 WB Sequence A6801

EFELRYRR 0.511 198 WB Sequence A6801

IAYQSFEQ 0.507 208 WB Sequence A6801

WDFLSYK 0.481 273 WB Sequence A6801

DEFELRYR 0.457 357 WB Sequence A6801

FSDVEENR 0.442 418 WB Sequence A6801

LLGSLFSR 0.430 476 WB Sequence A6801

EVIPMAAV 0.888 3 SB Sequence A6802

IVAGWLL 0.790 9 SB Sequence A6802

IGMIVAGV 0.742 16 SB Sequence A6802

MIVAGWL 0.697 26 SB Sequence A6802

MAAVKQAL 0.633 52 WB Sequence A6802

HLADSPAV 0.549 131 WB Sequence A6802

ERFNRWFL 0.481 273 WB Sequence A6802

DSPAVNGA 0.473 300 WB Sequence A6802

ELYGNNAA 0.447 395 WB Sequence A6802

EAGDEFEL 0.436 444 WB Sequence A6802 78 EVIPMAAV 0.812 7 SB Sequence A6901

57 HLADSPAV 0.740 16 SB Sequence A690192 ELYGNNAA 0.570 104 WB Sequence A690117 MTVAGWL 0.544 138 WB Sequence A690118 TVAGWLL 0.507 206 WB Sequence A6901

91 EAGDEFEL 0.489 252 WB Sequence A690153 SVDKEMQV 0.437 441 WB Sequence A690112 WFLTGMTV 0.436 445 WB Sequence A6901

61 SPAVNGAT 0.657 41 SB Sequence B0702

82 MAAVKQAL 0.468 316 WB Sequence B070266 AAWMATYL 0.430 477 WB Sequence B0702

97 ELRYRRAF 0.589 85 WB Sequence B0801

7 LWDFLSY 0.511 198 WB Sequence B150138 RIVAFFSF 0.493 240 WB Sequence B150112 HITPGTAY 0.492 243 WB Sequence B150165 IAAWMATY 0.473 300 WB Sequence B1501

97 ELRYRRAF 0.439 430 WB Sequence B150122 WLLGSLF 0.433 461 WB Sequence B150106 QERFNRWF 0.528 165 WB Sequence B1801

5 RELWDFL 0.517 185 WB Sequence B180122 FEQWNEL 0.508 205 WB Sequence B180110 NRWFLTGM 0.510 WB Sequence B270565 IAAWMATY 0.806 SB Sequence B3501

7 LWDFLSY 0.629 55 WB Sequence B3501

82 MAAVKQAL 0.591 83 WB Sequence B350112 HITPGTAY 0.543 140 WB Sequence B3501

75 DAREVIPM 0.516 187 WB Sequence B350142 FFSFGGAL 0.499 226 WB Sequence B3501

61 SPAVNGAT 0.478 283 WB Sequence B350166 AAWMATYL 0.476 289 WB Sequence B350117 MTVAGWL 0.470 307 WB Sequence B3501

5 RELWDFL 0.624 58 WB Sequence B400122 FEQWNEL 0.618 62 WB Sequence B4001

RELWDFL 0.442 420 WB Sequence B400256 KEMQVLVS 0.430 478 WB Sequence B4403

77 REVIPMAA 0.434 456 WB Sequence B450161 LVSRIAAW 0.626 57 WB Sequence B580165 IAAWMATY 0.593 81 WB Sequence B5801

16 LSQKGYSW 0.586 88 WB Sequence B5801

19 KGYSWSQF 0.543 141 WB Sequence B580138 RIVAFFSF 0.467 320 WB Sequence B5801

49 AINGNPSW 0.447 394 WB Sequence B5801

9.mers 04 FSDLTSQLH 0.482 270 WB Sequence AOlOl43 FSFGGALCV 0.518 183 WB Sequence A020117 MTVAGWLL 0.478 282 WB Sequence A020172 YLNDHLEPW 0.739 16 SB Sequence A020217 MTVAGWLL 0.604 72 WB Sequence A020265 IAAWMATYL 0.568 107 WB Sequence A020213 FLTGMTVAG 0.564 111 WB Sequence A0202

11 FLSYKLSQK 0.520 179 WB Sequence A020261 LVSRIAAWM 0.450 382 WB Sequence A0202 g WDFLSYKL 0.449 387 WB Sequence A020292 ELYGNNAAA 0.447 394 WB Sequence A0202

81 PMAAVKQAL 0.437 441 WB Sequence A020216 GMTVAGWL 0.436 448 WB Sequence A020214 LTGMTVAGV 0.691 28 SB Sequence A0203 217 MTVAGWLL 0.609 69 WB Sequence A0203

165 IAAWMATYL 0.530 161 WB Sequence A0203

84 AVKQALREA 0.518 183 WB Sequence A0203

110 QLHITPGTA 0.507 206 WB Sequence A0203

172 YLNDHLEPW 0.493 240 WB Sequence A0203

117 TAYQSFEQV 0.473 300 WB Sequence A0203

11 FLSYKLSQK 0.447 396 WB Sequence A0203

214 LTGMTVAGV 0.504 213 WB Sequence A0204

217 MTVAGWLL 0.475 291 WB Sequence A0204

109 SQLHITPGT 0.712 22 SB Sequence A0206

217 MTVAGWLL 0.675 33 SB Sequence A0206

117 TAYQSFEQV 0.650 43 SB Sequence A0206

1 SQSNRELW 0.648 45 SB Sequence A0206

143 FSFGGALCV 0.584 90 WB Sequence A0206

77 REVIPMAAV 0.572 103 WB Sequence A0206

165 IAAWMATYL 0.551 128 WB Sequence A0206

158 MQVLVSRIA 0.544 138 WB Sequence A0206

214 LTGMTVAGV 0.492 244 WB Sequence A0206

172 YLNDHLEPW 0.464 331 WB Sequence A0206

42 SEMETPSAI 0.440 426 WB Sequence A0206

192 ELYGNNAAA 0.863 4 SB Sequence A0211

143 FSFGGALCV 0.797 g SB Sequence A0211

81 PMAAVKQAL 0.794 9 SB Sequence A0211

172 YLNDHLEPW 0.715 21 SB Sequence A0211

153 SVDKEMQVL 0.703 24 SB Sequence A0211 g WDFLSYKL 0.696 26 SB Sequence A0211

217 MTVAGWLL 0.634 52 WB Sequence A0211

112 HITPGTAYQ 0.618 62 WB Sequence A0211

117 TAYQSFEQV 0.617 63 WB Sequence A0211

223 VLLGSLFSR 0.581 93 WB Sequence A0211

213 FLTGMTVAG 0.581 93 WB Sequence A0211

133 GVNWGRIVA 0.575 99 WB Sequence A0211

216 GMTVAGWL 0.553 126 WB Sequence A0211

185 GGWDTFVEL 0.550 130 WB Sequence A0211

103 AFSDLTSQL 0.472 302 WB Sequence A0211

176 HLEPWIQEN 0.427 493 WB Sequence A0211

192 ELYGNNAAA 0.845 5 SB Sequence A0212

81 PMAAVKQAL 0.789 9 SB Sequence A0212

143 FSFGGALCV 0.702 25 SB Sequence A0212

172 YLNDHLEPW 0.673 34 SB Sequence A0212

223 VLLGSLFSR 0.573 101 WB Sequence A0212 g WDFLSYKL 0.561 115 WB Sequence A0212

153 SVDKEMQVL 0.535 153 WB Sequence A0212

213 FLTGMTVAG 0.521 178 WB Sequence A0212

118 AYQSFEQW 0.476 290 WB Sequence A0212

192 ELYGNNAAA 0.741 16 SB Sequence A0216

81 PMAAVKQAL 0.710 22 SB Sequence A0216

143 FSFGGALCV 0.652 42 SB Sequence A0216

117 TAYQSFEQV 0.593 81 WB Sequence A0216

112 HITPGTAYQ 0.512 196 WB Sequence A0216

216 GMTVAGWL 0.430 479 WB Sequence A0216

81 PMAAVKQAL 0.675 33 SB Sequence A0219

143 FSFGGALCV 0.652 43 SB Sequence A0219

192 ELYGNNAAA 0.541 142 WB Sequence A0219

117 TAYQSFEQV 0.497 232 WB Sequence A0219

172 YLNDHLEPW 0.459 348 WB Sequence A0219

223 VLLGSLFSR 0.456 361 WB Sequence A0219

214 LTGMTVAGV 0.450 384 WB Sequence A0219

224 LLGSLFSRK 0.762 13 SB Sequence A0301

11 FLSYKLSQK 0.710 23 SB Sequence A0301

164 RIAAWMATY 0.698 26 SB Sequence A0301

223 VLLGSLFSR 0.615 64 WB Sequence A0301

7 LWDFLSYK 0.497 231 WB Sequence A0301

7 LWDFLSYK 0.767 12 SB Sequence AIlOl

224 LLGSLFSRK 0.612 66 WB Sequence AIlOl

223 VLLGSLFSR 0.595 79 WB Sequence AIlOl 164 RIAAWMATY 0.575 99 WB Sequence AIlOl

148 ALCVESVDK 0.529 163 WB Sequence AIlOl

78 EVIPMAAVK 0.509 201 WB Sequence AIlOl

11 FLSYKLSQK 0.430 477 WB Sequence AIlOl

99 RYRRAFSDL 0.690 28 SB Sequence A2301

135 NWGRIVAFF 0.644 47 SB Sequence A2301

137 GRIVAFFSF 0.459 346 WB Sequence A2301

135 NWGRIVAFF 0.739 16 SB Sequence A2402

99 RYRRAFSDL 0.550 129 WB Sequence A2402

99 RYRRAFSDL 0.748 15 SB Sequence A2403

121 SFEQWNEL 0.557 120 WB Sequence A2403

118 AYQSFEQW 0.487 256 WB Sequence A2403

6 ELWDFLSY 0.532 158 WB Sequence A2601

164 RIAAWMAlY 0.495 235 WB Sequence A2601

164 RIAAWMAlY 0.923 2 SB Sequence A2602

6 ELWDFLSY 0.873 3 SB Sequence A2602

161 LVSRIAAWM 0.677 32 SB Sequence A2602

153 SVDKEMQVL 0.639 49 SB Sequence A2602

78 EVIPMAAVK 0.496 234 WB Sequence A2602

217 MlVAGWLL 0.481 273 WB Sequence A2602

111 LHIlPGlAY 0.553 125 WB Sequence A2902

6 ELWDFLSY 0.539 146 WB Sequence A2902

164 RIAAWMAIY 0.463 334 WB Sequence A3002

82 MAAVKQALR 0.766 12 SB Sequence A3101

223 VLLGSLFSR 0.686 30 SB Sequence A3101

7 LWDFLSYK 0.573 101 WB Sequence A3101

156 KEMQVLVSR 0.474 296 WB Sequence A3101

94 DEFELRYRR 0.721 20 SB Sequence A3301

82 MAAVKQALR 0.665 37 SB Sequence A3301

97 ELRYRRAFS 0.614 65 WB Sequence A3301

223 VLLGSLFSR 0.581 92 WB Sequence A3301

91 EAGDEFELR 0.532 157 WB Sequence A3301

25 QFSDVEENR 0.531 159 WB Sequence A3301

78 EVIPMAAVK 0.848 5 SB Sequence A6801

82 MAAVKQALR 0.813 7 SB Sequence A6801

7 LWDFLSYK 0.786 10 SB Sequence A6801

91 EAGDEFELR 0.710 23 SB Sequence A6801

123 EQWNELFR 0.635 51 WB Sequence A6801

11 FLSYKLSQK 0 558 119 WB Sequence A6801

94 DEFELRYRR 0.544 139 WB Sequence A6801

25 QFSDVEENR 0.540 145 WB Sequence A6801

196 NNAAAESRK 0.474 295 WB Sequence A6801

223 VLLGSLFSR 0.430 477 WB Sequence A6801

217 MTVAGWLL 0.796 9 SB Sequence A6802

117 TAYQSFEQV 0.729 18 SB Sequence A6802

215 TGMTVAGW 0.654 42 SB Sequence A6802 o MSQSNRELV 0.587 86 WB Sequence A6802

21 YSWSQFSDV 0.549 131 WB Sequence A6802

143 FSFGGALCV 0.526 169 WB Sequence A6802

152 ESVDKEMQV 0.525 171 WB Sequence A6802

169 MATYLNDHL 0.520 180 WB Sequence A6802

192 ELYGNNAAA 0.509 202 WB Sequence A6802

140 VAFFSFGGA 0.500 222 WB Sequence A6802

214 LTGMTVAGV 0.464 330 WB Sequence A6802

165 IAAWMATYL 0.451 378 WB Sequence A6802

217 MTVAGWLL 0.705 24 SB Sequence A6901

117 TAYQSFEQV 0.623 58 WB Sequence A6901

192 ELYGNNAAA 0.604 72 WB Sequence A6901

143 FSFGGALCV 0.589 85 WB Sequence A6901

214 LTGMTVAGV 0.557 120 WB Sequence A6901

21 YSWSQFSDV 0.489 252 WB Sequence A6901 APEGTESEM 0.519 181 WB Sequence B0702

SPAVNGATG 0.454 369 WB Sequence B0702

TPGTAYQSF 0.450 382 WB Sequence B0702

FELRYRRAF 0.497 229 WB Sequence B0801

RIAAWMATY 0.586 87 WB Sequence B1501

ALREAGDEF 0.520 180 WB Sequence B1501

FELRYRRAF 0.752 14 SB Sequence B1801

QERFNRWFL 0.592 82 WB Sequence B1801

FEQWNELF 0.523 174 WB Sequence B1801

QENGGWDTF 0.476 290 WB Sequence B1801

GRIVAFFSF 0.554 124 WB Sequence B2705

RRAFSDLTS 0.434 459 WB Sequence B2705

TPGTAYQSF 0.705 24 SB Sequence B3501

IAAWMATYL 0.649 44 SB Sequence B3501

APEGTESEM 0.540 144 WB Sequence B3501

ELWDFLSY 0.531 159 WB Sequence B3501

LHITPGTAY 0.437 441 WB Sequence B3501

NPSWHLADS 0.429 480 WB Sequence B3501

RIAAWMATY 0.428 485 WB Sequence B3501

REAGDEFEL 0.788 9 SB Sequence B4001

QERFNRWFL 0.597 78 WB Sequence B4001

QENGGWDTF 0.525 170 WB Sequence B4001

FEQWNELF 0.453 370 WB Sequence B4001

FELRYRRAF 0.446 399 WB Sequence B4001

SEMETPSAI 0.504 215 WB Sequence B4002

FELRYRRAF 0.473 299 WB Sequence B4002

QENGGWDTF 0.434 455 WB Sequence B4402

SEMETPSAI 0.467 319 WB Sequence B4403

RELWDFLS 0.444 407 WB Sequence B4403

REVIPMAAV 0.438 438 WB Sequence B4501

IAAWMATYL 0.442 416 WB Sequence B5301

IPMAAVKQA 0.716 21 SB Sequence B5401

SAINGNPSW 0.641 48 SB Sequence B5801

KLSQKGYSW 0.596 79 WB Sequence B5801

IAAWMATYL 0.559 118 WB Sequence B5801

YLNDHLEPW 0.506 208 WB Sequence B5801

10-mers FSDLTSQLHI 0.427 492 WB Sequence AOIOI

YLNDHLEPWI 0.866 4 SB Sequence A0201

FLTGMTVAGV 0.841 5 SB Sequence A0201

RIAAWMATYL 0.651 43 SB Sequence A0201

WMATYLNDHL 0.573 101 WB Sequence A0201

LWDFLSYKL 0.524 173 WB Sequence A0201

SLDAREVIPM 0.491 246 WB Sequence A0201

VLVSRIAAWM 0.486 259 WB Sequence A0201

SVDKEMQVLV 0.473 298 WB Sequence A0201

GMTVAGWLL 0.444 411 WB Sequence A0201

FLTGMTVAGV 0.811 7 SB Sequence A0202

WMATYLNDHL 0.772 11 SB Sequence A0202

RIAAWMATYL 0.763 13 SB Sequence A0202

LWDFLSYKL 0.651 43 SB Sequence A0202

RAFSDLTSQL 0.617 63 WB Sequence A0202

SLDAREVIPM 0.616 63 WB Sequence A0202

YLNDHLEPWI 0.587 87 WB Sequence A0202

GMTVAGWLL 0.496 233 WB Sequence A0202

FGGALCVESV 0.480 276 WB Sequence A0202

VLVSRIAAWM 0.430 476 WB Sequence A0202 213 FLTGMTVAGV 0.936 2 SB Sequence A0203

172 YLNDHLEPWI 0.891 3 SB Sequence A0203

164 RIAAWMATYL 0.837 5 SB Sequence A0203

168 WMATYLNDHL 0.647 45 SB Sequence A0203

160 VLVSRIAAWM 0.613 66 WB Sequence A0203

139 IVAFFSFGGA 0.596 79 WB Sequence A0203

7 LWDFLSYKL 0.581 92 WB Sequence A0203

125 WNELFRDGV 0.570 105 WB Sequence A0203

216 GMTVAGWLL 0.476 289 WB Sequence A0203

102 RAFSDLTSQL 0.470 308 WB Sequence A0203

214 LTGMTVAGW 0.468 315 WB Sequence A0203

116 GTAYQSFEQV 0.463 335 WB Sequence A0203

213 FLTGMTVAGV 0.697 26 SB Sequence A0204

172 YLNDHLEPWI 0.664 37 SB Sequence A0204

73 SLDAREVIPM 0.477 287 WB Sequence A0204

164 RIAAWMATYL 0.476 290 WB Sequence A0204

7 LWDFLSYKL 0.468 317 WB Sequence A0204

49 AINGNPSWHL 0.452 374 WB Sequence A0204

213 FLTGMTVAGV 0.869 4 SB Sequence A0206

164 RIAAWMATYL 0.809 7 SB Sequence A0206

172 YLNDHLEPWI 0.722 20 SB Sequence A0206

158 MQVLVSRIAA 0.689 28 SB Sequence A0206

7 LWDFLSYKL 0.684 30 SB Sequence A0206

168 WMATYLNDHL 0.680 31 SB Sequence A0206

109 SQLHITPGTA 0.652 43 SB Sequence A0206

116 GTAYQSFEQV 0.572 102 WB Sequence A0206

153 SVDKEMQVLV 0.558 119 WB Sequence A0206

102 RAFSDLTSQL 0.543 140 WB Sequence A0206

156 KEMQVLVSRI 0.508 204 WB Sequence A0206

181 IQENGGWDTF 0.508 205 WB Sequence A0206

139 IVAFFSFGGA 0.495 235 WB Sequence A0206

125 WNELFRDGV 0.483 269 WB Sequence A0206

117 TAYQSFEQW 0.450 383 WB Sequence A0206

213 FLTGMTVAGV 0.966 1 SB Sequence A0211

172 YLNDHLEPWI 0.951 1 SB Sequence A0211

153 SVDKEMQVLV 0.905 2 SB Sequence A0211

73 SLDAREVIPM 0.826 6 SB Sequence A0211

216 GMTVAGWLL 0.737 17 SB Sequence A0211

7 LWDFLSYKL 0.730 18 SB Sequence A0211

164 RIAAWMATYL 0.711 22 SB Sequence A0211

125 WNELFRDGV 0.687 29 SB Sequence A0211

49 AINGNPSWHL 0.686 29 SB Sequence A0211

168 WMATYLNDHL 0.685 30 SB Sequence A0211

117 TAYQSFEQW 0.633 52 WB Sequence A0211

160 VLVSRIAAWM 0.632 53 WB Sequence A0211

142 FFSFGGALCV 0.567 107 WB Sequence A0211

223 VLLGSLFSRK 0.498 228 WB Sequence A0211

102 RAFSDLTSQL 0.453 372 WB Sequence A0211

116 GTAYQSFEQV 0.429 481 WB Sequence A0211

213 FLTGMTVAGV 0.932 2 SB Sequence A0212

172 YLNDHLEPWI 0.916 2 SB Sequence A0212

153 SVDKEMQVLV 0.742 16 SB Sequence A0212

168 WMATYLNDHL 0.697 26 SB Sequence A0212

125 WNELFRDGV 0.695 27 SB Sequence A0212

7 LWDFLSYKL 0.648 45 SB Sequence A0212

160 VLVSRIAAWM 0.604 72 WB Sequence A0212

73 SLDAREVIPM 0.594 80 WB Sequence A0212

49 AINGNPSWHL 0.570 104 WB Sequence A0212

164 RIAAWMATYL 0.550 129 WB Sequence A0212

142 FFSFGGALCV 0.494 238 WB Sequence A0212

223 VLLGSLFSRK 0.487 258 WB Sequence A0212

117 TAYQSFEQW 0.482 270 WB Sequence A0212

192 ELYGNNAAAE 0.475 293 WB Sequence A0212

216 GMTVAGWLL 0.440 426 WB Sequence A0212

213 FLTGMTVAGV 0.911 2 SB Sequence A0216

172 YLNDHLEPWI 0.869 4 SB Sequence A0216

153 SVDKEMQVLV 0.772 11 SB Sequence A0216

168 WMATYLNDHL 0.696 26 SB Sequence A0216 164 RIAAWMATYL 0.695 27 SB Sequence A0216

49 AINGNPSWHL 0.680 31 SB Sequence A0216

160 VLVSRIAAWM 0.657 41 SB Sequence A0216

7 LWDFLSYKL 0.643 47 SB Sequence A0216

73 SLDAREVIPM 0.617 62 WB Sequence A0216

216 GMlVAGWLL 0.588 85 WB Sequence A0216

117 IAYQSFEQW 0.530 161 WB Sequence A0216

142 FFSFGGALCV 0.487 256 WB Sequence A0216

116 G1AYQSFEQV 0.444 408 WB Sequence A0216

213 FLIGMlVAGV 0.927 2 SB Sequence A0219

172 YLNDHLEPWI 0.884 3 SB Sequence A0219

168 WMAIYLNDHL 0.611 67 WB Sequence A0219

49 AINGNPSWHL 0.543 140 WB Sequence A0219

7 LWDFLSYKL 0.539 146 WB Sequence A0219

153 SVDKEMQVLV 0.533 156 WB Sequence A0219

164 RIAAWMATYL 0.449 387 WB Sequence A0219

73 SLDAREVIPM 0.445 404 WB Sequence A0219

160 VLVSRIAAWM 0.441 421 WB Sequence A0219

223 VLLGSLFSRK 0.767 12 SB Sequence A0301

6 ELWDFLSYK 0.504 213 WB Sequence A0301

147 GALCVESVDK 0.488 253 WB Sequence A0301

222 WLLGSLFSR 0.457 356 WB Sequence A0301

77 REVIPMAAVK 0.453 372 WB Sequence A0301

223 VLLGSLFSRK 0.742 16 SB Sequence AIlOl

222 WLLGSLFSR 0.681 31 SB Sequence AIlOl

147 GALCVESVDK 0.515 190 WB Sequence AIlOl

24 SQFSDVEENR 0.470 310 WB Sequence AIlOl

121 SFEQWNELF 0.581 92 WB Sequence A2301

171 TYLNDHLEPW 0.547 134 WB Sequence A2301

121 SFEQWNELF 0.528 165 WB Sequence A2402

171 TYLNDHLEPW 0.520 180 WB Sequence A2402

113 ITPGTAYQSF 0.460 343 WB Sequence A2402

171 TYLNDHLEPW 0.739 16 SB Sequence A2403

121 SFEQWNELF 0.546 136 WB Sequence A2403

113 ITPGTAYQSF 0.508 204 WB Sequence A2403

35 EAPEGTESEM 0.448 390 WB Sequence A2601

164 RIAAWMATYL 0.626 57 WB Sequence A2602

113 ITPGTAYQSF 0.581 92 WB Sequence A2602

160 VLVSRIAAWM 0.553 126 WB Sequence A2602

35 EAPEGTESEM 0.507 207 WB Sequence A2602

152 ESVDKEMQVL 0.490 249 WB Sequence A2602

95 EFELRYRRAF 0.483 268 WB Sequence A2602

110 QLHITPGTAY 0.506 209 WB Sequence A2902

222 WLLGSLFSR 0.683 30 SB Sequence A3101

129 LFRDGVNWGR 0.667 36 SB Sequence A3101

202 SRKGQERFNR 0.608 69 WB Sequence A3101

81 PMAAVKQALR 0.521 177 WB Sequence A3101

222 WLLGSLFSR 0.572 103 WB Sequence A3301

129 LFRDGVNWGR 0.553 126 WB Sequence A3301

10 DFLSYKLSQK 0.470 308 WB Sequence A3301

6 ELWDFLSYK 0.702 25 SB Sequence A6801

24 SQFSDVEENR 0.532 158 WB Sequence A6801

222 WLLGSLFSR 0.516 188 WB Sequence A6801

194 YGNNAAAESR 0.493 240 WB Sequence A6801

78 EVIPMAAVKQ 0.454 368 WB Sequence A6801

169 MATYLNDHLE 0.448 394 WB Sequence A6801

139 IVAFFSFGGA 0.742 16 SB Sequence A6802

116 GTAYQSFEQV 0.673 34 SB Sequence A6802

7 LWDFLSYKL 0.659 39 SB Sequence A6802

120 QSFEQWNEL 0.618 62 WB Sequence A6802

213 FLTGMTVAGV 0.577 96 WB Sequence A6802 117 TAYQSFEQW 0.561 115 WB Sequence A6802

164 RIAAWMATYL 0.519 182 WB Sequence A6802

65 NGATGHSSSL 0.496 234 WB Sequence A6802

218 TVAGWLLGS 0.491 246 WB Sequence A6802

145 FGGALCVESV 0.474 294 WB Sequence A6802

125 WNELFRDGV 0.465 328 WB Sequence A6802

161 LVSRIAAWMA 0.451 380 WB Sequence A6802

215 TGMTVAGWL 0.450 382 WB Sequence A6802

153 SVDKEMQVLV 0.534 155 WB Sequence A6901

213 FLTGMTVAGV 0.527 166 WB Sequence A6901

117 TAYQSFEQW 0.466 324 WB Sequence A6901

7 LWDFLSYKL 0.451 378 WB Sequence A6901

164 RIAAWMATYL 0.443 412 WB Sequence A6901

116 GTAYQSFEQV 0.427 493 WB Sequence A6901

80 IPMAAVKQAL 0.704 24 SB Sequence B0702

17 SQKGYSWSQF 0.572 102 WB Sequence B1501

110 QLHITPGTAY 0.557 121 WB Sequence B1501

133 GVNWGRIVAF 0.548 132 WB Sequence B1501

181 IQENGGWDTF 0.522 175 WB Sequence B1501

12 LSYKLSQKGY 0.455 364 WB Sequence B1501

5 RELWDFLSY 0.759 13 SB Sequence B1801

163 SRIAAWMATY 0.588 86 WB Sequence B2705

101 RRAFSDLTSQ 0.461 340 WB Sequence B2705

80 IPMAAVKQAL 0.609 69 WB Sequence B3501

178 EPWIQENGGW 0.604 72 WB Sequence B3501

91 EAGDEFELRY 0.566 109 WB Sequence B3501

35 EAPEGTESEM 0.563 112 WB Sequence B3501

87 QALREAGDEF 0.508 206 WB Sequence B3501

61 SPAVNGATGH 0.490 249 WB Sequence B3501

46 TPSAINGNPS 0.483 269 WB Sequence B3501

133 GVNWGRIVAF 0.455 362 WB Sequence B3501

140 VAFFSFGGAL 0.454 367 WB Sequence B3501

190 FVELYGNNAA 0.439 430 WB Sequence B3501

200 AESRKGQERF 0.453 370 WB Sequence B4501

178 EPWIQENGGW 0.603 73 WB Sequence B5301

2 QSNRELWDF 0.474 295 WB Sequence B5801

159 QVLVSRIAAW 0.467 320 WB Sequence B5801

47 PSAINGNPSW 0.437 444 WB Sequence B5801

11-mers

213 FLTGMTVAGW 0.627 56 WB Sequence A0201

73 SLDAREVIPMA 0.561 115 WB Sequence A0201

160 VLVSRIAAWMA 0.547 135 WB Sequence A0201

57 HLADSPAVNGA 0.539 147 WB Sequence A0201

172 YLNDHLEPWIQ 0.480 278 WB Sequence A0201

88 ALREAGDEFEL 0.470 309 WB Sequence A0201

119 YQSFEQWNEL 0.426 497 WB Sequence A0201

57 HLADSPAVNGA 0.763 12 SB Sequence A0202

213 FLTGMTVAGW 0.754 14 SB Sequence A0202

119 YQSFEQWNEL 0.734 17 SB Sequence A0202

88 ALREAGDEFEL 0.679 32 SB Sequence A0202

172 YLNDHLEPWIQ 0.611 67 WB Sequence A0202

139 IVAFFSFGGAL 0 558 119 WB Sequence A0202

218 TVAGWLLGSL 0.537 149 WB Sequence A0202

160 VLVSRIAAWMA 0.525 170 WB Sequence A0202

15 KLSQKGYSWSQ 0.450 382 WB Sequence A0202

6 ELWDFLSYKL 0.449 387 WB Sequence A0202

73 SLDAREVIPMA 0.446 401 WB Sequence A0202

213 FLTGMTVAGW 0.864 4 SB Sequence A0203

57 HLADSPAVNGA 0.844 5 SB Sequence A0203

138 RIVAFFSFGGA 0.752 14 SB Sequence A0203 160 VLVSRIAAWMA 0.649 44 SB Sequence A0203

218 TVAGWLLGSL 0.619 61 WB Sequence A0203

88 ALREAGDEFEL 0.604 72 WB Sequence A0203

119 YQSFEQWNEL 0.567 108 WB Sequence A0203

172 YLNDHLEPWIQ 0.565 110 WB Sequence A0203

49 AINGNPSWHLA 0.562 114 WB Sequence A0203

209 FNRWFLTGMTV 0.494 239 WB Sequence A0203

139 IVAFFSFGGAL 0.487 257 WB Sequence A0203

73 SLDAREVIPMA 0.480 276 WB Sequence A0203

82 MAAVKQALREA 0.430 477 WB Sequence A0203

124 QWNELFRDGV 0.426 496 WB Sequence A0203

213 FLTGMTVAGW 0.584 90 WB Sequence A0204

88 ALREAGDEFEL 0.526 168 WB Sequence A0204

160 VLVSRIAAWMA 0.517 185 WB Sequence A0204

172 YLNDHLEPWIQ 0.517 186 WB Sequence A0204

73 SLDAREVIPMA 0.485 263 WB Sequence A0204

213 FLTGMTVAGW 0.746 15 SB Sequence A0206

138 RIVAFFSFGGA 0.725 19 SB Sequence A0206

181 IQENGGWDTFV 0.704 24 SB Sequence A0206

119 YQSFEQWNEL 0.671 35 SB Sequence A0206

48 SAINGNPSWHL 0.664 38 SB Sequence A0206

124 QWNELFRDGV 0.619 62 WB Sequence A0206

160 VLVSRIAAWMA 0.584 90 WB Sequence A0206

86 KQALREAGDEF 0.547 134 WB Sequence A0206

57 HLADSPAVNGA 0.509 201 WB Sequence A0206

218 TVAGWLLGSL 0.456 358 WB Sequence A0206

88 ALREAGDEFEL 0.442 420 WB Sequence A0206

109 SQLHITPGTAY 0.441 422 WB Sequence A0206

213 FLTGMTVAGW 0.943 1 SB Sequence A0211

73 SLDAREVIPMA 0.876 SB Sequence A0211

172 YLNDHLEPWIQ 0.852 4 SB Sequence A0211

88 ALREAGDEFEL 0.799 8 SB Sequence A0211

57 HLADSPAVNGA 0.787 10 SB Sequence A0211

160 VLVSRIAAWMA 0.759 13 SB Sequence A0211

15 KLSQKGYSWSQ 0.743 16 SB Sequence A0211

6 ELWDFLSYKL 0.682 31 SB Sequence A0211

218 TVAGWLLGSL 0.628 55 WB Sequence A0211

49 AINGNPSWHLA 0.612 66 WB Sequence A0211

212 WFLTGMTVAGV 0.577 97 WB Sequence A0211

141 AFFSFGGALCV 0.571 103 WB Sequence A0211

144 SFGGALCVESV 0.569 105 WB Sequence A0211

79 VIPMAAVKQAL 0.568 107 WB Sequence A0211

124 QWNELFRDGV 0.535 152 WB Sequence A0211

130 FRDGVNWGRIV 0.516 187 WB Sequence A0211

48 SAINGNPSWHL 0.515 189 WB Sequence A0211

192 ELYGNNAAAES 0.504 214 WB Sequence A0211

150 CVESVDKEMQV 0.489 252 WB Sequence A0211

116 GTAYQSFEQW 0.452 376 WB Sequence A0211

139 IVAFFSFGGAL 0.444 411 WB Sequence A0211

213 FLTGMTVAGW 0.824 6 SB Sequence A0212

172 YLNDHLEPWIQ 0.812 7 SB Sequence A0212

88 ALREAGDEFEL 0.779 10 SB Sequence A0212

73 SLDAREVIPMA 0.745 15 SB Sequence A0212

57 HLADSPAVNGA 0.714 22 SB Sequence A0212

160 VLVSRIAAWMA 0.681 31 SB Sequence A0212

79 VIPMAAVKQAL 0.604 72 WB Sequence A0212

15 KLSQKGYSWSQ 0.568 107 WB Sequence A0212

212 WFLTGMTVAGV 0.504 213 WB Sequence A0212

6 ELWDFLSYKL 0.451 380 WB Sequence A0212

130 FRDGVNWGRIV 0.431 473 WB Sequence A0212

213 FLTGMTVAGW 0.862 4 SB Sequence A0216

88 ALREAGDEFEL 0.821 6 SB Sequence A0216

73 SLDAREVIPMA 0.744 16 SB Sequence A0216

160 VLVSRIAAWMA 0.649 44 SB Sequence A0216

150 CVESVDKEMQV 0.641 48 SB Sequence A0216

57 HLADSPAVNGA 0.595 79 WB Sequence A0216

144 SFGGALCVESV 0.591 83 WB Sequence A0216

172 YLNDHLEPWIQ 0.581 92 WB Sequence A0216

15 KLSQKGYSWSQ 0.561 115 WB Sequence A0216 ELWDFLSYKL 0.544 138 WB Sequence A0216

TVAGWLLGSL 0 .534 154 WB Sequence A0216

AFFSFGGALCV 0 .526 168 WB Sequence A0216

IQENGGWDTFV 0 .497 231 WB Sequence A0216

QWNELFRDGV 0 .490 249 WB Sequence A0216

VIPMAAVKQAL 0 .487 257 WB Sequence A0216

ELYGNNAAAES 0 .478 283 WB Sequence A0216

SAINGNPSWHL 0 .468 315 WB Sequence A0216

WFLTGMTVAGV 0 .437 441 WB Sequence A0216

AINGNPSWHLA 0 .436 447 WB Sequence A0216

FLTGMTVAGW 0 .781 10 SB Sequence A0219

HLADSPAVNGA 0 .730 18 SB Sequence A0219

YLNDHLEPWIQ 0 .695 27 SB Sequence A0219

SLDAREVIPMA 0 .609 68 WB Sequence A0219

ALREAGDEFEL 0 .549 131 WB Sequence A0219

WFLTGMTVAGV 0 .524 172 WB Sequence A0219

VLVSRIAAWMA 0 .499 225 WB Sequence A0219

WLLGSLFSRK 0 .688 29 SB Sequence A0301

WLLGSLFSRK 0 .786 10 SB Sequence AIlOl

GWLLGSLFSR 0 .596 78 WB Sequence AIlOl

ATGHSSSLDAR 0 .505 212 WB Sequence AIlOl

RELWDFLSYK 0 .428 489 WB Sequence AIlOl

NWGRIVAFFSF 0 .695 26 SB Sequence A2301

TYLNDHLEPWI 0 .576 98 WB Sequence A2301

AWMATYLNDHL 0 .534 154 WB Sequence A2301

SYKLSQKGYSW 0 .531 159 WB Sequence A2301

NWGRIVAFFSF 0 .746 15 SB Sequence A2402

TYLNDHLEPWI 0 .746 15 SB Sequence A2402

AWMATYLNDHL 0 .520 180 WB Sequence A2402

TYLNDHLEPWI 0 .660 39 SB Sequence A2403

AWMATYLNDHL 0 .523 174 WB Sequence A2403

SYKLSQKGYSW 0 .523 174 WB Sequence A2403

HITPGTAYQSF 0 .431 473 WB Sequence A2403

QVLVSRIAAWM 0 .640 49 SB Sequence A2602

HITPGTAYQSF 0 .573 101 WB Sequence A2602

WIQENGGWDTF 0 .518 183 WB Sequence A2602

FLSYKLSQKGY 0 .516 188 WB Sequence A2602

DGVNWGRIVAF 0 .462 336 WB Sequence A2602

TVAGWLLGSL 0 .443 415 WB Sequence A2602

SQLHITPGTAY 0 .516 188 WB Sequence A2902

WLLGSLFSRK 0 .435 453 WB Sequence A3001

RYRRAFSDLTS 0 .428 486 WB Sequence A3001

GWLLGSLFSR 0 .598 77 WB Sequence A3101

SFEQWNELFR 0 .552 127 WB Sequence A3101

ESRKGQERFNR 0 .511 198 WB Sequence A3101

ATGHSSSLDAR 0 .475 292 WB Sequence A3101

RELWDFLSYK 0 .442 417 WB Sequence A3101

LYGNNAAAESR 0 .435 449 WB Sequence A3101

ESRKGQERFNR 0 .690 28 SB Sequence A3301

ELFRDGVNWGR 0 .634 52 WB Sequence A3301

EAGDEFELRYR 0 .538 148 WB Sequence A3301

SFEQWNELFR 0 .472 303 WB Sequence A3301

GWLLGSLFSR 0 .447 396 WB Sequence A3301

EFELRYRRAFS 0 .435 449 WB Sequence A3301

ELFRDGVNWGR 0 .739 16 SB Sequence A6801

WSQFSDVEENR 0 .667 36 SB Sequence A6801

ESRKGQERFNR 0 .666 36 SB Sequence A6801

EAGDEFELRYR 0 .643 47 SB Sequence A6801

GWLLGSLFSR 0 .638 49 SB Sequence A6801

IPMAAVKQALR 0 .566 109 WB Sequence A6801

SFEQWNELFR 0 .536 151 WB Sequence A6801 218 TVAGWLLGSL 0.796 9 SB Sequence A6802

124 QWNELFRDGV 0.712 22 SB Sequence A6802

139 IVAFFSFGGAL 0.684 30 SB Sequence A6802

152 ESVDKEMQVLV 0.613 65 WB Sequence A6802

215 TGMTVAGWLL 0.561 116 WB Sequence A6802

6 ELWDFLSYKL 0.551 129 WB Sequence A6802

138 RIVAFFSFGGA 0.548 132 WB Sequence A6802

188 DTFVELYGNNA 0.530 161 WB Sequence A6802

78 EVIPMAAVKQA 0.516 188 WB Sequence A6802

116 GTAYQSFEQW 0.514 191 WB Sequence A6802

75 DAREVIPMAAV 0.509 203 WB Sequence A6802

57 HLADSPAVNGA 0.508 206 WB Sequence A6802

217 MTVAGWLLGS 0.480 277 WB Sequence A6802

45 ETPSAINGNPS 0.479 279 WB Sequence A6802

213 FLTGMTVAGW 0.470 308 WB Sequence A6802

70 HSSSLDAREVI 0.447 397 WB Sequence A6802

48 SAINGNPSWHL 0.541 143 WB Sequence A6901

75 DAREVIPMAAV 0.527 166 WB Sequence A6901

152 ESVDKEMQVLV 0.511 199 WB Sequence A6901

57 HLADSPAVNGA 0.485 263 WB Sequence A6901

54 PSWHLADSPAV 0.469 312 WB Sequence A6901

212 WFLTGMTVAGV 0.453 369 WB Sequence A6901

78 EVIPMAAVKQA 0.442 417 WB Sequence A6901

6 ELWDFLSYKL 0.442 417 WB Sequence A6901

218 TVAGWLLGSL 0.430 475 WB Sequence A6901

188 DTFVELYGNNA 0.427 494 WB Sequence A6901

139 IVAFFSFGGAL 0.518 183 WB Sequence B0702

53 NPSWHLADSPA 0.499 224 WB Sequence B0702

61 SPAVNGATGHS 0.457 355 WB Sequence B0702

109 SQLHITPGTAY 0.605 72 WB Sequence B1501

86 KQALREAGDEF 0.567 108 WB Sequence B1501

1 SQSNRELWDF 0.540 144 WB Sequence B1501

119 YQSFEQWNEL 0.515 189 WB Sequence B1501

158 MQVLVSRIAAW 0.512 196 WB Sequence B1501

162 VSRIAAWMATY 0.477 285 WB Sequence B1501

11 FLSYKLSQKGY 0.474 294 WB Sequence B1501

180 WIQENGGWDTF 0.473 299 WB Sequence B1501

120 QSFEQWNELF 0.450 386 WB Sequence B1501

112 HITPGTAYQSF 0.447 394 WB Sequence B1501

133 GVNWGRIVAFF 0.433 463 WB Sequence B1501

94 DEFELRYRRAF 0.829 6 SB Sequence B1801

90 REAGDEFELRY 0.560 116 WB Sequence B1801

109 SQLHITPGTAY 0.539 146 WB Sequence B1801

151 VESVDKEMQVL 0.476 288 WB Sequence B1801

177 LEPWIQENGGW 0.466 321 WB Sequence B1801

209 FNRWFLTGMTV 0.444 409 WB Sequence B1801

101 RRAFSDLTSQL 0.563 113 WB Sequence B2705

163 SRIAAWMATYL 0.470 310 WB Sequence B2705

53 NPSWHLADSPA 0.623 59 WB Sequence B3501

46 TPSAINGNPSW 0.485 263 WB Sequence B3501

180 WIQENGGWDTF 0.474 297 WB Sequence B3501

132 DGVNWGRIVAF 0.442 419 WB Sequence B3501

119 YQSFEQWNEL 0.610 68 WB Sequence B3901

151 VESVDKEMQVL 0.492 243 WB Sequence B4001

40 TESEMETPSAI 0.446 402 WB Sequence B4002

30 EENRTEAPEGT 0.498 228 WB Sequence B4501

46 TPSAINGNPSW 0.772 11 SB Sequence B5301

53 NPSWHLADSPA 0.430 474 WB Sequence B5401

170 ATYLNDHLEPW 0.540 145 WB Sequence B5701

170 ATYLNDHLEPW 0.537 150 WB Sequence B5801 120 QSFEQWNELF 0.495 235 WB Sequence B5801

Table 8: Prediction of cancer antigen BcIX(L) specific MHC class 2, 15-mer peptide binders.

Prediction of cancer antigen BcIX(L) specific MHC class 2, 15-mer peptide binders for 14 MHC class 2 alleles using the http://www.cbs.dtu.dk/services/NetMHCII / database. The MHC class 2 molecules for which no binders were found are not listed.

Allele pos peptide l-log50k(aff) affinity (nM) Bind Level Identity

DRBlJlOl 212 RWFLTGMTVAGWLL LTGMTVAGV 0.8028 8 SB BcIX(L)

DRBlJlOl 209 RFNRWFLTGMTVAGV FLTGMTVAG 0.7932 9 SB BdX(L)

DRBlJlOl 210 FNRWFLTGMTVAGW LTGMTVAGV 0.7940 9 SB BdX(L)

DRBlJlOl 211 NRWFLTGMTVAGWL LTGMTVAGV 0.7970 9 SB BdX(L)

DRBlJlOl 213 WFLTGMTVAGWLLG LTGMTVAGV 0.7753 11 SB BdX(L)

DRBlJlOl 76 DAREVIPMAAVKQAL VIPMAAVKQ 0.7755 11 SB BdX(L)

DRBlJlOl 77 AREVIPMAAVKQALR VIPMAAVKQ 0.7788 11 SB BdX(L)

DRBlJlOl 78 REVIPMAAVKQALRE VIPMAAVKQ 0.7772 11 SB BdX(L)

DRBlJlOl 75 LDAREVIPMAAVKQA VIPMAAVKQ 0.7730 12 SB BdX(L)

DRBlJlOl 157 KEMQVLVSRIAAWMA MQVLVSRIA 0.7458 16 SB BdX(L)

DRBlJlOl 108 LTSQLHITPGTAYQS LHITPGTAY 0.7338 18 SB BdX(L)

DRBlJlOl 109 TSQLHITPGTAYQSF ITPGTAYQS 0.7313 18 SB BdX(L)

DRBlJlOl 74 SLDAREVIPMAAVKQ AREVIPMAA 0.7348 18 SB BdX(L)

DRBlJlOl 110 SQLHITPGTAYQSFE ITPGTAYQS 0.7287 19 SB BdX(L)

DRBlJlOl 214 FLTGMTVAGWLLGS LTGMTVAGV 0.7282 19 SB BdX(L)

DRBlJlOl 156 DKEMQVLVSRIAAWM MQVLVSRIA 0.7226 20 SB BdX(L)

DRBlJlOl 111 QLHITPGTAYQSFEQ ITPGTAYQS 0.7202 21 SB BdX(L)

DRBlJlOl 112 LHITPGTAYQSFEQV ITPGTAYQS 0.7189 21 SB BdX(L)

DRBlJlOl 154 SVDKEMQVLVSRIAA MQVLVSRIA 0.7165 21 SB BdX(L)

DRBlJlOl 79 EVIPMAAVKQALREA IPMAAVKQA 0.7208 21 SB BdX(L)

DRBlJlOl 153 ESVDKEMQVLVSRIA VDKEMQVLV 0.7145 22 SB BdX(L)

DRBlJlOl 155 VDKEMQVLVSRIAAW MQVLVSRIA 0.7141 22 SB BdX(L)

DRBlJlOl 215 LTGMTVAGWLLGSL LTGMTVAGV 0.7090 23 SB BdX(L)

DRBlJlOl 208 ERFNRWFLTGMTVAG FNRWFLTGM 0.7077 24 SB BdX(L)

DRBlJlOl 46 ETPSAINGNPSWHLA INGNPSWHL 0.7051 24 SB BdX(L)

DRBlJlOl 47 TPSAINGNPSWHLAD INGNPSWHL 0.7051 24 SB BdX(L)

DRBlJlOl 48 PSAINGNPSWHLADS INGNPSWHL 0.7076 24 SB BdX(L)

DRBlJlOl 49 SAINGNPSWHLADSP INGNPSWHL 0.7072 24 SB BdX(L)

DRBlJlOl 45 METPSAINGNPSWHL PSAINGNPS 0.7034 25 SB BdX(L)

DRBlJlOl 158 EMQVLVSRIAAWMAT MQVLVSRIA 0.6845 30 SB BdX(L)

DRBlJlOl 80 VIPMAAVKQALREAG VIPMAAVKQ 0.6856 30 SB BdX(L)

DRBlJlOl 159 MQVLVSRIAAWMATY MQVLVSRIA 0.6834 31 SB BdX(L)

DRBlJlOl 161 VLVSRIAAWMATYLN IAAWMATYL 0.6838 31 SB BdX(L)

DRBlJlOl 217 GMTVAGWLLGSLFS MTVAGWLL 0.6800 32 SB BdX(L)

DRBlJlOl 218 MTVAGWLLGSLFSR WLLGSLFS 0.6800 32 SB BdX(L)

DRBlJlOl 51 INGNPSWHLADSPAV INGNPSWHL 0.6778 33 SB BdX(L)

DRBlJlOl 192 VELYGNNAAAESRKG YGNNAAAES 0.6693 36 SB BdX(L)

DRBlJlOl 219 TVAGWLLGSLFSRK WLLGSLFS 0.6677 36 SB BdX(L)

DRBlJlOl 160 QVLVSRIAAWMATYL VSRIAAWMA 0.6652 37 SB BdX(L)

DRBlJlOl 191 FVELYGNNAAAESRK YGNNAAAES 0.6658 37 SB BdX(L)

DRBlJlOl 193 ELYGNNAAAESRKGQ YGNNAAAES 0.6661 37 SB BdX(L)

DRBlJlOl 99 LRYRRAFSDLTSQLH YRRAFSDLT 0.6657 37 SB BdX(L)

DRBlJlOl 162 LVSRIAAWMATYLND IAAWMATYL 0.6627 38 SB BdX(L)

DRBlJlOl 190 TFVELYGNNAAAESR YGNNAAAES 0.6628 38 SB BdX(L)

DRBlJlOl 189 DTFVELYGNNAAAES FVELYGNNA 0.6613 39 SB BdX(L)

DRBlJlOl 54 NPSWHLADSPAVNGA WHLADSPAV 0.6617 39 SB BdX(L)

DRBlJlOl 55 PSWHLADSPAVNGAT WHLADSPAV 0.6611 39 SB BdX(L)

DRBlJlOl 216 TGMTVAGWLLGSLF MTVAGWLL 0.6543 42 SB BdX(L)

DRBlJlOl 163 VSRIAAWMATYLNDH IAAWMATYL 0.6531 43 SB BdX(L)

DRBlJlOl 53 GNPSWHLADSPAVNG WHLADSPAV 0.6532 43 SB BdX(L)

DRBlJlOl 59 LADSPAVNGATGHSS LADSPAVNG 0.6361 51 WB BdX(L)

DRBlJlOl 98 ELRYRRAFSDLTSQL YRRAFSDLT 0.6357 51 WB BdX(L)

DRBlJlOl 164 SRIAAWMATYLNDHL IAAWMATYL 0.6357 52 WB BdX(L)

DRBlJlOl 97 FELRYRRAFSDLTSQ YRRAFSDLT 0.6337 53 WB BdX(L)

DRBlJlOl 96 EFELRYRRAFSDLTS YRRAFSDLT 0.6321 54 WB BdX(L)

DRBlJlOl 95 DEFELRYRRAFSDLT LRYRRAFSD 0.6294 55 WB BdX(L)

DRBlJlOl 140 IVAFFSFGGALCVES FSFGGALCV 0.6203 61 WB BdX(L)

DRBl 0101 56 SWHLADSPAVNGATG LADSPAVNG 0.6196 61 WB BdX(L) DRBlJlOl 61 DSPAVNGATGHSSSL VNGATGHSS 0.6197 61 WB BdX(L)

DRBlJlOl 62 SPAVNGATGHSSSLD VNGATGHSS O .6205 61 WB BdX(L)

DRBlJlOl 141 VAFFSFGGALCVESV FSFGGALCV O .6191 62 WB BdX(L)

DRBlJlOl 60 ADSPAVNGATGHSSS VNGATGHSS O .6174 63 WB BdX(L)

DRBlJlOl 142 AFFSFGGALCVESVD FSFGGALCV 0.6153 64 WB BdX(L)

DRBlJlOl 57 WHLADSPAVNGATGH LADSPAVNG 0.6162 64 WB BdX(L)

DRBlJlOl 113 HITPGTAYQSFEQW ITPGTAYQS 0.6121 66 WB BdX(L)

DRBlJlOl 114 ITPGTAYQSFEQWN ITPGTAYQS 0.6131 66 WB BdX(L)

DRBlJlOl 50 AINGNPSWHLADSPA INGNPSWHL O .6133 66 WB BdX(L)

DRBlJlOl 63 PAVNGATGHSSSLDA VNGATGHSS 0.6078 70 WB BdX(L)

DRBlJlOl 100 RYRRAFSDLTSQLHI YRRAFSDLT O .6026 74 WB BdX(L)

DRBlJlOl 101 YRRAFSDLTSQLHIT YRRAFSDLT O .6021 74 WB BdX(L)

DRBlJlOl 52 NGNPSWHLADSPAVN WHLADSPAV 0.6004 75 WB BdX(L)

DRBlJlOl 138 GRIVAFFSFGGALCV IVAFFSFGG O .5864 88 WB BdX(L)

DRBlJlOl 194 LYGNNAAAESRKGQE YGNNAAAES O .5808 93 WB BdX(L)

DRBlJlOl 165 RIAAWMATYLNDHLE AAWMATYLN O .5762 98 WB BdX(L)

DRBlJlOl 195 YGNNAAAESRKGQER YGNNAAAES O .5731 101 WB BdX(L)

DRBlJlOl 139 RIVAFFSFGGALCVE FSFGGALCV O .5729 102 WB BdX(L)

DRBlJlOl 131 FRDGVNWGRIVAFFS VNWGRIVAF 5627 114 WB BdX(L)

DRBlJlOl 143 FFSFGGALCVESVDK FSFGGALCV 5613 115 WB BdX(L)

DRBlJlOl 144 FSFGGALCVESVDKE FSFGGALCV 5583 119 WB BdX(L)

DRBlJlOl 132 RDGVNWGRIVAFFSF VNWGRIVAF 5561 122 WB BdX(L)

DRBlJlOl 133 DGVNWGRIVAFFSFG VNWGRIVAF 5558 122 WB BdX(L)

DRBlJlOl 81 IPMAAVKQALREAGD IPMAAVKQA O .5527 126 WB BdX(L)

DRBlJlOl 102 RRAFSDLTSQLHITP FSDLTSQLH O .5334 156 WB BdX(L)

DRBlJlOl 103 RAFSDLTSQLHITPG FSDLTSQLH 0.5314 159 WB BdX(L)

DRBlJlOl 58 HLADSPAVNGATGHS LADSPAVNG O .5293 163 WB BdX(L)

DRBlJlOl 134 GVNWGRIVAFFSFGG VNWGRIVAF U .5ZHZ 165 WB BdX(L)

DRBlJlOl 166 IAAWMATYLNDHLEP IAAWMATYL O .5271 167 WB BdX(L)

DRBlJlOl 64 AVNGATGHSSSLDAR VNGATGHSS 0.5266 168 WB BdX(L)

DRBlJlOl 135 VNWGRIVAFFSFGGA VNWGRIVAF O .5248 171 WB BdX(L)

DRBlJlOl 7 ELWDFLSYKLSQKG WDFLSYKL O .5243 172 WB BdX(L)

DRBlJlOl 129 ELFRDGVNWGRIVAF FRDGVNWGR 0.5154 189 WB BdX(L)

DRBlJlOl 65 VNGATGHSSSLDARE VNGATGHSS O .5156 189 WB BdX(L)

DRBlJlOl 130 LFRDGVNWGRIVAFF VNWGRIVAF O .5023 218 WB BdX(L)

DRBlJlOl 8 LWDFLSYKLSQKGY LSYKLSQKG O .4921 244 WB BdX(L)

DRBlJlOl 9 WDFLSYKLSQKGYS LSYKLSQKG O .4892 251 WB BdX(L)

DRBlJlOl 207 QERFNRWFLTGMTVA FNRWFLTGM O .4845 264 WB BdX(L)

DRBlJlOl 42 ESEMETPSAINGNPS METPSAING O .4626 335 WB BdX(L)

DRBlJlOl 40 GTESEMETPSAINGN METPSAING 0.4622 337 WB BdX(L)

DRBlJlOl 107 DLTSQLHITPGTAYQ LHITPGTAY O .4593 347 WB BdX(L)

DRBlJlOl 39 EGTESEMETPSAING ESEMETPSA 0.4591 348 WB BdX(L)

DRBlJlOl 43 SEMETPSAINGNPSW METPSAING O .4590 348 WB BdX(L)

DRBlJlOl 106 SDLTSQLHITPGTAY SQLHITPGT O .4569 356 WB BdX(L)

DRBlJlOl 10 VDFLSYKLSQKGYSW LSYKLSQKG O .4565 358 WB BdX(L)

DRBlJlOl 41 TESEMETPSAINGNP METPSAING O .4564 358 WB BdX(L)

DRBlJlOl 167 AAWMATYLNDHLEPW AAWMATYLN O .4556 361 WB BdX(L)

DRBlJlOl 11 DFLSYKLSQKGYSWS LSYKLSQKG 0.4515 378 WB BdX(L)

DRBlJlOl 104 AFSDLTSQLHITPGT FSDLTSQLH O .4453 404 WB BdX(L)

DRBlJlOl 105 FSDLTSQLHITPGTA FSDLTSQLH 0.4451 405 WB BdX(L)

DRBlJlOl 137 WGRIVAFFSFGGALC IVAFFSFGG 0.4311 471 WB BdX(L)

DRBlJlOl 206 GQERFNRWFLTGMTV FNRWFLTGM O .4277 489 WB BdX(L)

DRBlJ 401 99 LRYRRAFSDLTSQLH YRRAFSDLT O .5618 115 WB BdX(L)

DRBlJ 401 97 FELRYRRAFSDLTSQ YRRAFSDLT 0.5300 162 WB BdX(L)

DRBlJ 401 96 EFELRYRRAFSDLTS YRRAFSDLT O .5284 164 WB BdX(L)

DRBlJ 401 95 DEFELRYRRAFSDLT DEFELRYRR O .5275 166 WB BdX(L)

DRBlJ 401 98 ELRYRRAFSDLTSQL YRRAFSDLT O .5259 169 WB BdX(L)

DRBlJ 401 185 NGGWDTFVELYGNNA WDTFVELYG O .5189 182 WB BdX(L)

DRBlJ 401 186 GGWDTFVELYGNNAA FVELYGNNA O .5180 184 WB BdX(L)

DRBlJ 401 188 WDTFVELYGNNAAAE FVELYGNNA 0.5159 188 WB BdX(L)

DRBlJ 401 187 GWDTFVELYGNNAAA FVELYGNNA 0.5157 189 WB BdX(L)

DRBlJ 401 189 DTFVELYGNNAAAES FVELYGNNA O .5154 189 WB BdX(L)

DRBlJ 401 100 RYRRAFSDLTSQLHI YRRAFSDLT O .4844 WB BdX(L)

DRBlJ 401 101 YRRAFSDLTSQLHIT YRRAFSDLT O .4813 274 WB BdX(L)

DRBlJ 401 153 ESVDKEMQVLVSRIA KEMQVLVSR 0.4561 360 WB BdX(L)

DRBlJ 401 155 VDKEMQVLVSRIAAW MQVLVSRIA 0.4519 376 WB BdX(L)

DRBlJ 401 208 ERFNRWFLTGMTVAG WFLTGMTVA 0.4512 379 WB BdX(L)

DRBlJ 401 154 SVDKEMQVLVSRIAA MQVLVSRIA O .4495 386 WB BdX(L)

DRBlJ 401 209 RFNRWFLTGMTVAGV FLTGMTVAG O .4467 398 WB BdX(L)

DRBlJ 401 210 FNRWFLTGMTVAGW FLTGMTVAG O .4427 416 WB BdX(L)

DRBlJ 401 157 KEMQVLVSRIAAWMA MQVLVSRIA O .4419 419 WB BdX(L)

DRBlJ 401 156 DKEMQVLVSRIAAWM MQVLVSRIA 0.4413 422 WB BdX(L)

DRBl 0401 211 NRWFLTGMTVAGWL FLTGMTVAG O .4327 463 WB BdX(L) DRBl_0404 167 AAWMATYLNDHLEPW WMATYLNDH 0.5484 132 WB BcIX(L)

DRBl_0404 164 SRIAAWMATYLNDHL WMATYLNDH 0.5424 141 WB BdX(L)

DRBl_0404 165 RIAAWMATYLNDHLE WMATYLNDH 0.5417 142 WB BdX(L)

DRBl_0404 166 IAAWMATYLNDHLEP WMATYLNDH 0.5330 156 WB BdX(L)

DRBl_0404 163 VSRIAAWMATYLNDH AAWMATYLN 0.5217 177 WB BdX(L)

DRBl_0404 219 TVAGWLLGSLFSRK WLLGSLFS 0.5094 202 WB BdX(L)

DRBl_0404 209 RFNRWFLTGMTVAGV FLTGMTVAG 0.4902 249 WB BdX(L)

DRBl_0404 210 FNRWFLTGMTVAGW FLTGMTVAG 0.4853 262 WB BdX(L)

DRBl_0404 211 NRWFLTGMTVAGWL FLTGMTVAG 0.4826 270 WB BdX(L)

DRBl_0404 208 ERFNRWFLTGMTVAG WFLTGMTVA 0.4761 290 WB BdX(L)

DRBl_0404 168 AWMATYLNDHLEPWI WMATYLNDH 0.4694 311 WB BdX(L)

DRBl_0404 212 RWFLTGMTVAGWLL FLTGMTVAG 0.4547 365 WB BdX(L)

DRBl_0404 189 DTFVELYGNNAAAES FVELYGNNA 0.4505 382 WB BdX(L)

DRBl_0404 187 GWDTFVELYGNNAAA FVELYGNNA 0.4498 385 WB BdX(L)

DRBl_0404 169 WMATYLNDHLEPWIQ WMATYLNDH 0.4478 393 WB BdX(L)

DRBl_0404 188 WDTFVELYGNNAAAE FVELYGNNA 0.4462 400 WB BdX(L)

DRBl_0404 186 GGWDTFVELYGNNAA FVELYGNNA 0.4437 411 WB BdX(L)

DRBl_0404 185 NGGWDTFVELYGNNA GWDTFVELY 0.4388 434 WB BdX(L)

DRBl_0405 118 TAYQSFEQWNELFR YQSFEQWN 0.5794 WB BdX(L)

DRBl_0405 117 GTAYQSFEQWNELF YQSFEQWN 0.5772 97 WB BdX(L)

DRBl_0405 115 TPGTAYQSFEQWNE YQSFEQWN 0.5541 124 WB BdX(L)

DRBl_0405 116 PGTAYQSFEQWNEL YQSFEQWN 0.5538 125 WB BdX(L)

DRBl_0405 114 ITPGTAYQSFEQWN AYQSFEQW 0.5505 129 WB BdX(L)

DRBl_0405 163 VSRIAAWMATYLNDH AAWMATYLN 0.5510 129 WB BdX(L)

DRBl_0405 162 LVSRIAAWMATYLND AAWMATYLN 0.5473 134 WB BdX(L)

DRBl_0405 161 VLVSRIAAWMATYLN IAAWMATYL 0.5442 139 WB BdX(L)

DRBl_0405 164 SRIAAWMATYLNDHL AAWMATYLN 0.5402 145 WB BdX(L)

DRBl_0405 99 LRYRRAFSDLTSQLH YRRAFSDLT 0.5100 201 WB BdX(L)

DRBl_0405 97 FELRYRRAFSDLTSQ YRRAFSDLT 0.5085 204 WB BdX(L)

DRBl_0405 96 EFELRYRRAFSDLTS YRRAFSDLT 0.5069 208 WB BdX(L)

DRBl_0405 165 RIAAWMATYLNDHLE AAWMATYLN 0.5055 211 WB BdX(L)

DRBl_0405 119 AYQSFEQWNELFRD YQSFEQWN 0.4974 230 WB BdX(L)

DRBl_0405 120 YQSFEQWNELFRDG YQSFEQWN 0.4968 231 WB BdX(L)

DRBl_0405 98 ELRYRRAFSDLTSQL YRRAFSDLT 0.4923 243 WB BdX(L)

DRBl_0405 18 SQKGYSWSQFSDVEE GYSWSQFSD 0.4575 354 WB BdX(L)

DRBl_0405 95 DEFELRYRRAFSDLT LRYRRAFSD 0.4574 355 WB BdX(L)

DRBl_0405 19 QKGYSWSQFSDVEEN WSQFSDVEE 0.4562 359 WB BdX(L)

DRBl_0405 100 RYRRAFSDLTSQLHI YRRAFSDLT 0.4475 395 WB BdX(L)

DRBl_0405 20 KGYSWSQFSDVEENR WSQFSDVEE 0.4430 414 WB BdX(L)

DRBl_0405 166 IAAWMATYLNDHLEP AAWMATYLN 0.4423 418 WB BdX(L)

DRBl_0405 21 GYSWSQFSDVEENRT WSQFSDVEE 0.4353 450 WB BdX(L)

DRBl_0701 157 KEMQVLVSRIAAWMA VLVSRIAAW n 175 WB BdX(L)

DRBl_0701 159 MQVLVSRIAAWMATY VLVSRIAAW 0.5194 181 WB BdX(L)

DRBl_0701 158 EMQVLVSRIAAWMAT VLVSRIAAW 0.5191 182 WB BdX(L)

DRBl_0701 156 DKEMQVLVSRIAAWM VLVSRIAAW 0.4971 231 WB BdX(L)

DRBl_0701 160 QVLVSRIAAWMATYL VLVSRIAAW 0.4833 268 WB BdX(L)

DRBl_0701 46 ETPSAINGNPSWHLA INGNPSWHL 0.4783 283 WB BdX(L)

DRBl_0701 45 METPSAINGNPSWHL AINGNPSWH 0.4779 284 WB BdX(L)

DRBl_0701 155 VDKEMQVLVSRIAAW MQVLVSRIA 0.4772 286 WB BdX(L)

DRBl_0701 47 TPSAINGNPSWHLAD INGNPSWHL 0.4774 286 WB BdX(L)

DRBl_0701 48 PSAINGNPSWHLADS INGNPSWHL 0.4764 289 WB BdX(L)

DRBl_0701 161 VLVSRIAAWMATYLN VLVSRIAAW 0.4745 295 WB BdX(L)

DRBl_0701 49 SAINGNPSWHLADSP INGNPSWHL 0.4718 303 WB BdX(L)

DRBl_0701 99 LRYRRAFSDLTSQLH YRRAFSDLT 0.4627 335 WB BdX(L)

DRBl_0701 100 RYRRAFSDLTSQLHI FSDLTSQLH 0.4416 420 WB BdX(L)

DRBl_0701 101 YRRAFSDLTSQLHIT FSDLTSQLH 0.4344 455 WB BdX(L)

DRBl_0701 51 INGNPSWHLADSPAV INGNPSWHL 0.4291 481 WB BdX(L)

DRBl_0901 55 PSWHLADSPAVNGAT WHLADSPAV 0.5482 133 WB BdX(L)

DRBl_0901 54 NPSWHLADSPAVNGA WHLADSPAV 0.5414 143 WB BdX(L)

DRBl_0901 53 GNPSWHLADSPAVNG WHLADSPAV 0.5408 144 WB BdX(L)

DRBl_0901 51 INGNPSWHLADSPAV SWHLADSPA 0.5289 164 WB BdX(L)

DRBl_0901 52 NGNPSWHLADSPAVN WHLADSPAV 0.5284 164 WB BdX(L)

DRBl_0901 56 SWHLADSPAVNGATG WHLADSPAV 0.4678 317 WB BdX(L)

DRBl_0901 57 WHLADSPAVNGATGH WHLADSPAV 0.4636 331 WB BdX(L)

DRBl_0901 212 RWFLTGMTVAGWLL FLTGMTVAG 0.4483 391 WB BdX(L)

DRBl_0901 213 WFLTGMTVAGWLLG MTVAGWLL 0.4445 408 WB BdX(L)

DRBl_0901 214 FLTGMTVAGWLLGS MTVAGWLL 0.4327 463 WB BdX(L)

DRB1_11O1 157 KEMQVLVSRIAAWMA MQVLVSRIA 0.4319 467 WB BdX(L)

DRBl 1101 131 FRDGVNWGRIVAFFS GVNWGRIVA 0.4310 472 WB BdX(L) DRB1_11O1 156 DKEMQVLVSRIAAWM MQVLVSRIA 0.4308 473 WB BdX(L) DRB1_11O1 155 VDKEMQVLVSRIAAW MQVLVSRIA 0.4292 481 WB BdX(L) DRB1_11O1 132 RDGVNWGRIVAFFSF GVNWGRIVA 0.4283 486 WB BdX(L) DRBl 1101 154 SVDKEMQVLVSRIAA MQVLVSRIA 0.4267 494 WB BdX(L)

DRB1_ 1302 218 MTVAGWLLGSLFSR WLLGSLFS 0.4945 237 WB BdX(L) DRB1_ 1302 216 TGMTVAGWLLGSLF MTVAGWLL 0.4855 262 WB BdX(L) DRB1_ 1302 214 FLTGMTVAGWLLGS MTVAGWLL 0.4842 265 WB BdX(L) DRB1_ 1302 217 GMTVAGWLLGSLFS MTVAGWLL 0.4840 266 WB BdX(L) DRB1_ 1302 212 RWFLTGMTVAGWLL FLTGMTVAG 0.4832 268 WB BdX(L) DRB1_ 1302 215 LTGMTVAGWLLGSL MTVAGWLL 0.4775 285 WB BdX(L) DRB1_ 1302 213 WFLTGMTVAGWLLG MTVAGWLL 0.4716 304 WB BdX(L) DRB1_ 1302 189 DTFVELYGNNAAAES VELYGNNAA 0.4688 313 WB BdX(L) DRB1_ 1302 190 TFVELYGNNAAAESR YGNNAAAES 0.4615 339 WB BdX(L) DRB1_ 1302 191 FVELYGNNAAAESRK YGNNAAAES 0.4526 373 WB BdX(L) DRB1_ 1302 192 VELYGNNAAAESRKG YGNNAAAES 0.4415 421 WB BdX(L) DRB1_ 1302 219 TVAGWLLGSLFSRK WLLGSLFS 0.4384 436 WB BdX(L) DRB1_ 1302 193 ELYGNNAAAESRKGQ YGNNAAAES 0.4275 490 WB BdX(L)

DRB1_ _.1501 219 TVAGWLLGSLFSRK VLLGSLFSR 0.6681 36 SB BdX(L) DRB1_ _.1501 218 MTVAGWLLGSLFSR WLLGSLFS 0.6441 47 SB BdX(L) DRB1_ _.1501 6 RELWDFLSYKLSQK LWDFLSYK 0.5208 179 WB BdX(L) DRB1_ 1501 7 ELWDFLSYKLSQKG FLSYKLSQK 0.5009 221 WB BdX(L) DRB1_ 1501 157 KEMQVLVSRIAAWMA MQVLVSRIA 0.4968 231 WB BdX(L) DRB1_ 1501 156 DKEMQVLVSRIAAWM MQVLVSRIA 0.4965 232 WB BdX(L) DRB1_ 1501 209 RFNRWFLTGMTVAGV FNRWFLTGM 0.4870 257 WB BdX(L) DRB1_ _.1501 164 SRIAAWMATYLNDHL WMATYLNDH 0.4849 263 WB BdX(L) DRB1_ _.1501 210 FNRWFLTGMTVAGW LTGMTVAGV 0.4836 267 WB BdX(L) DRB1_ _.1501 8 LWDFLSYKLSQKGY FLSYKLSQK 0.4778 284 WB BdX(L) DRB1_ _.1501 5 NRELWDFLSYKLSQ LWDFLSYK 0.4777 285 WB BdX(L) DRB1_ 1501 135 VNWGRIVAFFSFGGA IVAFFSFGG 0.4756 291 WB BdX(L) DRB1_ 1501 4 SNRELWDFLSYKLS LWDFLSYK 0.4755 291 WB BdX(L) DRB1_ 1501 159 MQVLVSRIAAWMATY LVSRIAAWM 0.4693 312 WB BdX(L) DRB1_ 1501 163 VSRIAAWMATYLNDH IAAWMATYL 0.4691 312 WB BdX(L) DRB1_ _.1501 158 EMQVLVSRIAAWMAT VLVSRIAAW 0.4683 315 WB BdX(L) DRB1_ _.1501 165 RIAAWMATYLNDHLE WMATYLNDH 0.4685 315 WB BdX(L) DRB1_ _.1501 128 NELFRDGVNWGRIVA LFRDGVNWG 0.4675 318 WB BdX(L) DRB1_ _.1501 125 QWNELFRDGVNWGR LFRDGVNWG 0.4668 320 WB BdX(L) DRB1_ _.1501 126 WNELFRDGVNWGRI LFRDGVNWG 0.4668 320 WB BdX(L) DRB1_ 1501 166 IAAWMATYLNDHLEP WMATYLNDH 0.4649 327 WB BdX(L) DRB1_ 1501 137 WGRIVAFFSFGGALC IVAFFSFGG 0.4643 329 WB BdX(L) DRB1_ 1501 136 NWGRIVAFFSFGGAL IVAFFSFGG 0.4638 331 WB BdX(L) DRB1_ 1501 207 QERFNRWFLTGMTVA FNRWFLTGM 0.4608 342 WB BdX(L) DRB1_ _.1501 208 ERFNRWFLTGMTVAG FNRWFLTGM 0.4602 344 WB BdX(L) DRB1_ _.1501 138 GRIVAFFSFGGALCV IVAFFSFGG 0.4587 350 WB BdX(L) DRB1_ _.1501 9 WDFLSYKLSQKGYS FLSYKLSQK 0.4584 351 WB BdX(L) DRB1_ _.1501 155 VDKEMQVLVSRIAAW MQVLVSRIA 0.4570 356 WB BdX(L) DRB1_ 1501 167 AAWMATYLNDHLEPW WMATYLNDH 0.4551 364 WB BdX(L) DRB1_ 1501 3 QSNRELWDFLSYKL LWDFLSYK 0.4543 367 WB BdX(L) DRB1_ 1501 127 VNELFRDGVNWGRIV LFRDGVNWG 0.4431 414 WB BdX(L) DRB1_ 1501 134 GVNWGRIVAFFSFGG VNWGRIVAF 0.4391 432 WB BdX(L) DRB1_ _.1501 129 ELFRDGVNWGRIVAF LFRDGVNWG 0.4390 433 WB BdX(L) DRB1_ _.1501 217 GMTVAGWLLGSLFS VAGWLLGS 0.4377 439 WB BdX(L) DRB1_ _.1501 124 EQWNELFRDGVNWG WNELFRDG 0.4373 441 WB BdX(L) DRB1_ _.1501 139 RIVAFFSFGGALCVE IVAFFSFGG 0.4300 477 WB BdX(L) DRB1_ 1501 211 NRWFLTGMTVAGWL LTGMTVAGV 0.4264 496 WB BdX(L)

DRB4_ 0101 99 LRYRRAFSDLTSQLH YRRAFSDLT 0.4654 325 WB BdX(L) DRB4_ 0101 100 RYRRAFSDLTSQLHI FSDLTSQLH 0.4328 463 WB BdX(L)

Table 9: CMV antigenic peptides. MHC class I or MHC class Il antigenic peptides derived from CMV antigens. The sequences of the CMV antigens are also listed in the figure.

Protein and peptide sequences SEQ ID NO

<YP_081531.1 tegument protein pp65;Human herpesvirus 5> 1 -4401

MESRGRRCPEMISVLGPISGHVLKAVFSRGDTPVLPHETRLLQTGIHVRVSQPSLILV SQYTPDSTPCHRGDNQLQVQHTYFTGSEVENVSVNVHNPTGRSICPSQEPMSIYVY ALPLKMLN IPSINVHHYPSAAERKHRHLPVADAVIHASGKQMWQARLTVSGLAWTR

QQNQWKEPDVYYTSAFVFPTKDVALRHVVCAHELVCSMENTRATKMQVIGDQYVK

VYLESFCEDVPSGKLFMHVTLGSDVEEDLTMTRNPQPFMRPHERNGFTVLCPKNMII

KPGKISHIMLDVAFTSHEHFGLLCPKSIPGLSISGNLLMNGQQIFLEVQAIRETVELRQ

YDPVAALFFFDIDLLLQRGPQYSEHPTFTSQYRIQGKLEYRHTWDRHDEGAAQGDD

DVWTSGSDSDEELVTTERKTPRVTGGGAMASASTSAGRKRKSASSATACTAGVMT

RGRLKAESTVAPEEDTDEDSDNEIHNPAVFTWPPWQAGILARNLVPMVATVQGQNL

KYQEFFWDANDIYRIFAELEGVWQPAAQPKRRRHRQDALPGPCIASTPKKHRG

8 mers:

MESRGRRCiESRGRRCPiSRGRRCPEiRGRRCPEMiGRRCPEMIiRRCPEMISiRCPE

MISViCPEMISVLiPEMISVLGiEMISVLGPiMISVLGPlilSVLGPISiSVLGPISGiVLGPIS

GHiLGPISGHViGPISGHVLiPISGHVLKiISGHVLKAiSGHVLKAViGHVLKAVFiHVLKA

VFSiVLKAVFSRiLKAVFSRGiKAVFSRGDiAVFSRGDTiVFSRGDTPiFSRGDTPViSR

GDTPVLiRGDTPVLPiGDTPVLPHiDTPVLPHEiTPVLPHETiPVLPHETRiVLPHETRL;

LPHETRLL;PHETRLLQ;HETRLLQT;ETRLLQTG;TRLLQTGI;RLLQTGIH;LLQTGIHV;

LQTGIHVRiQTGIHVRViTGIHVRVSiGIHVRVSQiIHVRVSQPiHVRVSQPSiVRVSQPS

LiRVSQPSLIiVSQPSLILiSQPSLILViQPSLILVSiPSLILVSQiSLILVSQYiLILVSQYTiIL

VSQYTPiLVSQYTPDiVSQYTPDSiSQYTPDSTiQYTPDSTPiYTPDSTPCiTPDSTPCH

;PDSTPCHR;DSTPCHRG;STPCHRGD;TPCHRGDN;PCHRGDNQ;CHRGDNQL;HRG

DNQLQ;RGDNQLQV;GDNQLQVQ;DNQLQVQH;NQLQVQHT;QLQVQHTY;LQVQHT

YF;QVQHTYFT;VQHTYFTG;QHTYFTGS;HTYFTGSE;TYFTGSEV;YFTGSEVE;FTG

SEVENiTGSEVENViGSEVENVSiSEVENVSViEVENVSVNiVENVSVNViENVSVNVH;

NVSVNVHNiVSVNVHNPiSVNVHNPTiVNVHNPTGiNVHNPTGRiVHNPTGRSiHNPT

GRSIiNPTGRSICiPTGRSICPiTGRSICPSiGRSICPSQiRSICPSQEiSICPSQEPiICPS

QEPMiCPSQEPMSiPSQEPMSIiSQEPMSIYiQEPMSIYViEPMSIYVYiPMSIYVYAiMS

IYVYALiSIYVYALPiIYVYALPLiYVYALPLKiVYALPLKMiYALPLKMLiALPLKMLNiLPL

KMLNIiPLKMLNIPiLKMLNIPSiKMLNIPSIiMLNIPSINiLNIPSINViNIPSINVHiIPSINVH

HiPSINVHHYiSINVHHYPiINVHHYPSiNVHHYPSAiVHHYPSAAiHHYPSAAEiHYPSA

AER;YPSAAERK;PSAAERKH;SAAERKHR;AAERKHRH;AERKHRHL;ERKHRHLP;R

KHRHLPViKHRHLPVAiHRHLPVADiRHLPVADAiHLPVADAViLPVADAVIiPVADAVIH

;VADAVIHA;ADAVIHAS;DAVIHASG;AVIHASGK;VIHASGKQ;IHASGKQM;HASGKQM

W;ASGKQMWQ;SGKQMWQA;GKQMWQAR;KQMWQARL;QMWQARLT;MWQARLT

ViWQARLTVSiQARLTVSGiARLTVSGLiRLTVSGLAiLTVSGLAWiTVSGLAWTiVSGL

AWTRiSGLAWTRQiGLAWTRQQiLAWTRQQNiAWTRQQNQiWTRQQNQWiTRQQN

QWK;RQQNQWKE;QQNQWKEP;QNQWKEPD;NQWKEPDV;QW KEPDVYiWKEPDV

YYiKEPDVYYTiEPDVYYTSiPDVYYTSAiDVYYTSAFiVYYTSAFViYYTSAFVFiYTSA

FVFPiTSAFVFPTiSAFVFPTKiAFVFPTKDiFVFPTKDViVFPTKDVAiFPTKDVALiPTK

DVALRiTKDVALRHiKDVALRHViDVALRHVViVALRHVVCiALRHVVCAiLRHVVCAH;

RHVVCAHEiHVVCAHELiVVCAHELViVCAHELVCiCAHELVCSiAHELVCSMiHELVC

SMEiELVCSMENiLVCSMENTiVCSMENTRiCSMENTRAiSMENTRATiMENTRATKiE

NTRATKMiNTRATKMQiTRATKMQViRATKMQVIiATKMQVIGiTKMQVIGDiKMQVIG

DQiMQVIGDQYiQVIGDQYViVIGDQYVKiIGDQYVKViGDQYVKVYiDQYVKVYLiQYV

KVYLEiYVKVYLESiVKVYLESFiKVYLESFCiVYLESFCEiYLESFCEDiLESFCEDViES

FCEDVPiSFCEDVPSiFCEDVPSGiCEDVPSGKiEDVPSGKLiDVPSGKLFiVPSGKLF

MiPSGKLFMHiSGKLFMHViGKLFMHVTiKLFMHVTLiLFMHVTLGiFMHVTLGSiMHVT

LGSD;HVTLGSDV;VTLGSDVE;TLGSDVEE;LGSDVEED;GSDVEEDL;SDVEEDLT;D

VEEDLTM;VEEDLTMT;EEDLTMTR;EDLTMTRN;DLTMTRNP;LTMTRNPQ;TMTRNP

QP;MTRNPQPF;TRNPQPFM;RNPQPFMR;NPQPFMRP;PQPFMRPH;QPFMRPHE;P

FMRPHERiFMRPHERN iMRPHERNGiRPHERNGFiPHERNGFTiHERNGFTViERNG

FTVLiRNGFTVLCiNGFTVLCPiGFTVLCPKiFTVLCPKNiTVLCPKNMiVLCPKNMIiLCP

KNMIIiCPKNMIIKiPKNMIIKPiKNMIIKPGiNMIIKPGKiMIIKPGKIiIIKPGKISiIKPGKISH;

KPGKISHhPGKISHIMiGKISHIMLiKISH IMLDJSHIMLDViSHIMLDVAiHIMLDVAFiIML

DVAFTiMLDVAFTSiLDVAFTSHiDVAFTSHEiVAFTSHEHiAFTSHEHFiFTSHEHFGiT

SHEHFGLiSHEHFGLLiHEHFGLLCiEHFGLLCPiHFGLLCPKiFGLLCPKSiGLLCPKSI;

LLCPKSIPiLCPKSIPGiCPKSIPGLiPKSIPGLSiKSIPGLSIiSIPGLSISilPGLSISGiPGLS

ISGNiGLSISGNLiLSISGNLLiSISGNLLMiISGNLLMNiSGNLLMNGiGNLLMNGQiNLL

MNGQQiLLMNGQQIiLMNGQQIFiMNGQQIFLiNGQQIFLEiGQQIFLEViQQIFLEVQiQ IFLEVQAiIFLEVQAIiFLEVQAIRiLEVQAIREiEVQAIRETiVQAIRETViQAIRETVEiAIRE

TVELiIRETVELRiRETVELRQiETVELRQYiTVELRQYDiVELRQYDPiELRQYDPViLR

QYDPVAiRQYDPVAAiQYDPVAALiYDPVAALFiDPVAALFFiPVAALFFFiVAALFFFD;

AALFFFDIiALFFFDIDiLFFFDIDLiFFFDIDLLiFFDIDLLLiFDIDLLLQiDIDLLLQRiIDLLL

QRG;DLLLQRGP;LLLQRGPQ;LLQRGPQY;LQRGPQYS;QRGPQYSE;RGPQYSEH;

GPQYSEHPiPQYSEHPTiQYSEHPTFiYSEHPTFTiSEHPTFTSiEHPTFTSQiHPTFTS

QYiPTFTSQYRiTFTSQYRIiFTSQYRIQiTSQYRIQGiSQYRIQGKiQYRIQGKLiYRIQG

KLE;RIQGKLEY;IQGKLEYR;QGKLEYRH;GKLEYRHT;KLEYRHTW;LEYRHTWD;EY

RHTWDR;YRHTWDRH;RHTWDRHD;HTWDRHDE;TWDRHDEG;WDRHDEGA;DRH

DEGAA;RHDEGAAQ;HDEGAAQG;DEGAAQGD;EGAAQGDD;GAAQGDDD;AAQGD

DDV;AQGDDDVW;QGDDDVWT;GDDDVWTS;DDDVWTSG;DDVWTSGS;DVWTSG

SDiVWTSGSDSiWTSGSDSDiTSGSDSDEiSGSDSDEEiGSDSDEELiSDSDEELViDS

DEELVTiSDEELVTTiDEELVTTEiEELVTTERiELVTTERKiLVTTERKTiVTTERKTPiTT

ERKTPRiTERKTPRViERKTPRVTiRKTPRVTGiKTPRVTGGiTPRVTGGGiPRVTGGG

AiRVTGGGAMiVTGGGAMAiTGGGAMASiGGGAMASAiGGAMASASiGAMASASTiA

MASASTSiMASASTSAiASASTSAGiSASTSAGRiASTSAGRKiSTSAGRKRiTSAGRK

RK;SAGRKRKS;AGRKRKSA;GRKRKSAS;RKRKSASS;KRKSASSA;RKSASSAT;KS

ASSATAiSASSATACiASSATACTiSSATACTAiSATACTAGiATACTAGViTACTAGVM;

ACTAGVMTiCTAGVMTRiTAGVMTRGiAGVMTRGRiGVMTRGRLiVMTRGRLKiMTR

GRLKAiTRGRLKAEiRGRLKAESiGRLKAESTiRLKAESTViLKAESTVAiKAESTVAPiA

ESTVAPEiESTVAPEEiSTVAPEEDiTVAPEEDTiVAPEEDTDiAPEEDTDEiPEEDTDE

DiEEDTDEDSiEDTDEDSDiDTDEDSDNiTDEDSDNEiDEDSDNEIiEDSDNEIHiDSDN

EIHNiSDNEIHNPiDNEIHNPAiNEIHNPAViEIHNPAVFiIHNPAVFTiHNPAVFTWiNPAV

FTWP;PAVFTWPP;AVFTWPPW;VFTWPPWQ;FTWPPWQA;TWPPWQAG;WPPWQ

AGIiPPWQAGILiPWQAGILAiWQAGILARiQAGILARNiAGILARNLiGILARNLViILARN

LVPiLARNLVPMiARNLVPMViRNLVPMVAiNLVPMVATiLVPMVATViVPMVATVQiPM

VATVQG;MVATVQGQ;VATVQGQN;ATVQGQNL;TVQGQNLK;VQGQNLKY;QGQNL

KYQ;GQNLKYQE;QNLKYQEF;NLKYQEFF;LKYQEFFW;KYQEFFWD;YQEFFWDA;Q

EFFWDANiEFFWDANDiFFWDANDIiFWDANDIYiWDANDIYRiDANDIYRIiANDIYRIF;

NDIYRIFAiDIYRIFAEJYRIFAELiYRIFAELEiRIFAELEGJFAELEGViFAELEGVWiAEL

EGVWQiELEGVWQPiLEGVWQPAiEGVWQPAAiGVWQPAAQiVWQPAAQPiWQPA

AQPK;QPAAQPKR;PAAQPKRR;AAQPKRRR;AQPKRRRH;QPKRRRHR;PKRRRHR

Q;KRRRHRQD;RRRHRQDA;RRHRQDAL;RHRQDALP;HRQDALPG;RQDALPGP;QD

ALPGPCiDALPGPCIiALPGPCIAiLPGPCIASiPGPCIASTiGPCIASTPiPCIASTPKiCIA

STPKK;IASTPKKH;ASTPKKHR;STPKKHRG;

9 ΓΠΘΓS^"

MESRGRRCPiESRGRRCPEiSRGRRCPEMiRGRRCPEMIiGRRCPEMlSiRRCPEMl

SViRCPEMISVLiCPEMISVLGiPEMISVLGPiEMISVLGPIiMISVLGPISiISVLGPISGiSV

LGPISGHiVLGPISGHViLGPISGHVLiGPISGHVLKiPISGHVLKAiISGHVLKAViSGHVL

KAVFiGHVLKAVFSiHVLKAVFSRiVLKAVFSRGiLKAVFSRGDiKAVFSRGDTiAVFSR

GDTPiVFSRGDTPViFSRGDTPVLiSRGDTPVLPiRGDTPVLPHiGDTPVLPHEiDTPVL

PHET;TPVLPHETR;PVLPHETRL;VLPHETRLL;LPHETRLLQ;PHETRLLQT;HETRLLQ

TGiETRLLQTGIiTRLLQTGIHiRLLQTGIHViLLQTGIHVRiLQTGIHVRViQTGIHVRVSiT

GIHVRVSQiGIHVRVSQPiIHVRVSQPSiHVRVSQPSLiVRVSQPSLIiRVSQPSLILiVS

QPSLILViSQPSLILVSiQPSLILVSQiPSLILVSQYiSLILVSQYTiLILVSQYTPiILVSQYT

PDiLVSQYTPDSiVSQYTPDSTiSQYTPDSTPiQYTPDSTPCiYTPDSTPCHiTPDSTPC

HR;PDSTPCHRG;DSTPCHRGD;STPCHRGDN;TPCHRGDNQ;PCHRGDNQL;CHRG

DNQLQ;HRGDNQLQV;RGDNQLQVQ;GDNQLQVQH;DNQLQVQHT;NQLQVQHTY;Q

LQVQHTYFiLQVQHTYFTiQVQHTYFTGiVQHTYFTGSiQHTYFTGSEiHTYFTGSEViT

YFTGSEVEiYFTGSEVENiFTGSEVENViTGSEVENVSiGSEVENVSViSEVENVSVN;

EVENVSVNViVENVSVNVHiENVSVNVHNiNVSVNVHNPiVSVNVHNPTiSVNVHNPT

GiVNVHNPTGRiNVHNPTGRSiVHNPTGRSIiHNPTGRSICiNPTGRSICPiPTGRSICP

SiTGRSICPSQiGRSICPSQEiRSICPSQEPiSICPSQEPMiICPSQEPMSiCPSQEPMSI;

PSQEPMSIYiSQEPMSIYViQEPMSIYVYiEPMSIYVYAiPMSIYVYALiMSIYVYALPiSIY

VYALPLiIYVYALPLKiYVYALPLKMiVYALPLKMLiYALPLKMLN iALPLKMLNIiLPLKML

NIPiPLKMLNIPSiLKMLNIPSIiKMLN IPSINiMLNIPSINViLNIPSINVHiN IPSINVHHiIPSI

NVHHYiPSINVHHYPiSINVHHYPSiINVHHYPSAiNVHHYPSAAiVHHYPSAAEiHHYP SAAER;HYPSAAERK;YPSAAERKH;PSAAERKHR;SAAERKHRH;AAERKHRHL;AER

KHRHLPiERKHRHLPVjRKHRHLPVAiKHRHLPVADiHRHLPVADAjRHLPVADAViHL

PVADAVIjLPVADAVIHiPVADAVIHAiVADAVIHASiADAVIHASGiDAVIHASGKjAVIHA

SGKQ;VIHASGKQM;IHASGKQMW;HASGKQMWQ;ASGKQMWQA;SGKQMWQAR;

GKQMWQARL;KQMWQARLT;QMWQARLTV;MWQARLTVS;WQARLTVSG;QARLTV

SGLiARLTVSGLAjRLTVSGLAWiLTVSGLAWTjTVSGLAWTRiVSGLAWTRQiSGLAW

TRQQ;GLAWTRQQN;LAWTRQQNQ;AWTRQQNQW;WTRQQNQWK;TRQQNQWKE

;RQQNQWKEP;QQNQWKEPD;QNQWKEPDV;NQWKEPDVY;QWKEPDVYY;WKEP

DVYYTjKEPDVYYTSiEPDVYYTSAiPDVYYTSAFiDVYYTSAFViVYYTSAFVFjYYTSA

FVFPiYTSAFVFPTjTSAFVFPTKiSAFVFPTKDiAFVFPTKDVjFVFPTKDVAiVFPTKDV

ALjFPTKDVALRiPTKDVALRHiTKDVALRHViKDVALRHVVjDVALRHVVCiVALRHVV

CAiALRHVVCAHjLRHVVCAHEiRHVVCAHELjHVVCAHELViVVCAHELVCiVCAHEL

VCSjCAHELVCSMjAHELVCSMEiHELVCSMENjELVCSMENTiLVCSMENTRiVCSM

ENTRAjCSMENTRATiSMENTRATKiMENTRATKMiENTRATKMQiNTRATKMQVjTR

ATKMQVIiRATKMQVIGiATKMQVIGDiTKMQVIGDQiKMQVIGDQYiMQVIGDQYViQV

IGDQYVKiVIGDQYVKViIGDQYVKVYiGDQYVKVYLiDQYVKVYLEiQYVKVYLESiYV

KVYLESFiVKVYLESFCiKVYLESFCEiVYLESFCEDiYLESFCEDViLESFCEDVPiESF

CEDVPSiSFCEDVPSGiFCEDVPSGKiCEDVPSGKLiEDVPSGKLFiDVPSGKLFMiVP

SGKLFMHiPSGKLFMHViSGKLFMHVTiGKLFMHVTLiKLFMHVTLGiLFMHVTLGSiF

MHVTLGSDiMHVTLGSDViHVTLGSDVEiVTLGSDVEEiTLGSDVEEDiLGSDVEEDL;

GSDVEEDLT;SDVEEDLTM;DVEEDLTMT;VEEDLTMTR;EEDLTMTRN;EDLTMTRNP

;DLTMTRNPQ;LTMTRNPQP;TMTRNPQPF;MTRNPQPFM;TRNPQPFMR;RNPQPFM

RPiNPQPFMRPH iPQPFMRPHEiQPFMRPHERiPFMRPHERNiFMRPHERNGiMRPH

ERNGFiRPHERNGFTiPHERNGFTViHERNGFTVLiERNGFTVLCiRNGFTVLCPiNGF

TVLCPKiGFTVLCPKN iFTVLCPKNMiTVLCPKNMIiVLCPKNMIIiLCPKNMIIKiCPKNMI

IKPiPKNMIIKPGiKNMIIKPGKiNMIIKPGKIiMIIKPGKISiIIKPGKISHiIKPGKISHIiKPGKI

SHIMiPGKISHIMLiGKISHIMLDiKISHIMLDViISH IMLDVAiSHIMLDVAFiHIMLDVAFTiI

MLDVAFTSiMLDVAFTSHiLDVAFTSHEiDVAFTSHEH iVAFTSHEHFiAFTSHEHFGiF

TSHEHFGLiTSHEHFGLLiSHEHFGLLCiHEHFGLLCPiEHFGLLCPKiHFGLLCPKSiF

GLLCPKSliGLLCPKSIPiLLCPKSIPGiLCPKSIPGLiCPKSIPGLSiPKSIPGLSIiKSIPGL

SISiSIPGLSISGiIPGLSISGNiPGLSISGNLiGLSISGNLLiLSISGNLLMiSISGNLLMNiIS

GNLLMNG;SGNLLMNGQ;GNLLMNGQQ;NLLMNGQQI;LLMNGQQIF;LMNGQQIFL;M

NGQQIFLEiNGQQIFLEViGQQIFLEVQiQQIFLEVQAiQIFLEVQAIiIFLEVQAIRiFLEVQ

AIREiLEVQAIRETiEVQAIRETViVQAIRETVEiQAIRETVELiAIRETVELRiIRETVELRQ

;RETVELRQY;ETVELRQYD;TVELRQYDP;VELRQYDPV;ELRQYD PVA;LRQYDPVA

AiRQYDPVAALiQYDPVAALFiYDPVAALFFiDPVAALFFFiPVAALFFFDiVAALFFFDI;

AALFFFDIDiALFFFDIDLiLFFFDIDLLiFFFDIDLLLiFFDIDLLLQiFDIDLLLQRiDIDLLLQ

RGiIDLLLQRGPiDLLLQRGPQiLLLQRGPQYiLLQRGPQYSiLQRGPQYSEiQRGPQY

SEHiRGPQYSEHPiGPQYSEHPTiPQYSEHPTFiQYSEHPTFTiYSEHPTFTSiSEHPT

FTSQiEHPTFTSQYiHPTFTSQYRiPTFTSQYRIiTFTSQYRIQiFTSQYRIQGiTSQYRIQ

GK;SQYRIQGKL;QYRIQGKLE;YRIQGKLEY;RIQG KLEYR;IQGKLEYRH;QGKLEYRH

T;GKLEYRHTW;KLEYRHTWD;LEYRHTWDR;EYRHTWDRH;YRHTWDRHD;RHTWD

RHDE;HTWDRHDEG;TWDRHDEGA;WDRHDEGAA;DRHDEGAAQ;RHDEGAAQG;H

DEGAAQGD;DEGAAQGDD;EGAAQGDDD;GAAQGDDDV;AAQGDDDVW;AQGDDD

VWTiQGDDDVWTSiGDDDVWTSGiDDDVWTSGSiDDVWTSGSDiDVWTSGSDSiV

WTSGSDSDiWTSGSDSDEiTSGSDSDEEiSGSDSDEELiGSDSDEELViSDSDEELVT

;DSDEELVTT;SDEELVTTE;DEELVTTER;EELVTTERK;ELVTTERKT;LVTTERKTP;V

TTERKTPRiTTERKTPRViTERKTPRVTiERKTPRVTGiRKTPRVTGGiKTPRVTGGGiT

PRVTGGGA;PRVTGGGAM;RVTGGGAMA;VTGGGAMAS;TGGGAMASA;GGGAMAS

AS;GGAMASAST;GAMASASTS ;AM ASASTSA;MASASTSAG ;ASASTSAG R;SASTSA

GRKiASTSAGRKRiSTSAGRKRKiTSAGRKRKSiSAGRKRKSAiAGRKRKSASiGRKR

KSASSiRKRKSASSAiKRKSASSATiRKSASSATAiKSASSATACiSASSATACTiASSA

TACTAiSSATACTAG ;SATACTAGV;ATACTAGVM;TACTAGVMT;ACTAGVMTR;CTA

GVMTRG;TAGVMTRGR;AGVMTRGRL;GVMTRGRLK;VMTRGRLKA;MTRGRLKAE;

TRGRLKAESiRGRLKAESTiGRLKAESTViRLKAESTVAiLKAESTVAPiKAESTVAPEiA

ESTVAPEEiESTVAPEEDiSTVAPEEDTiTVAPEEDTDiVAPEEDTDEiAPEEDTDEDiP

EEDTDEDSiEEDTDEDSDiEDTDEDSDNiDTDEDSDNEiTDEDSDNEIiDEDSDNEIHiE

DSDNEIHNiDSDNEIHNPiSDNEIHNPAiDNEIHNPAViNEIHNPAVFiEIHNPAVFTiIHN O

PAVFTW;HNPAVFTWP;NPAVFTWPP;PAVFTWPPW;AVFTWPPWQ;VFTWPPWQA;

FTWPPWQAGiTWPPWQAGIiWPPWQAGILjPPWQAGILAiPWQAGILARjWQAGILAR

NiQAGILARNLjAGILARNLViGILARNLVPilLARNLVPMiLARNLVPMVjARNLVPMVA;

RNLVPMVAT;NLVPMVATV;LVPMVATVQ;VPMVATVQG;PMVATVQGQ;MVATVQG

QN;VATVQGQNL;ATVQGQNLK;TVQGQNLKY;VQGQNLKYQ;QGQNLKYQE;GQNLK

YQEF;QNLKYQEFF;NLKYQEFFW;LKYQEFFWD;KYQEFFWDA;YQEFFWDAN;QEF

FWDANDjEFFWDANDIiFFWDANDIYiFWDANDIYRiWDANDIYRIiDANDIYRIFjANDI

YRIFAiNDIYRIFAEjDIYRIFAELilYRIFAELEjYRIFAELEGiRIFAELEGVilFAELEGVW;

FAELEGVWQ;AELEGVWQP;ELEGVWQPA;LEGVWQPAA;EGVWQPAAQ;GVWQPA

AQP;VWQPAAQPK;WQPAAQPKR;QPAAQPKRR;PAAQPKRRR;AAQPKRRRH;AQP

KRRRHR;QPKRRRHRQ;PKRRRHRQD;KRRRHRQDA;RRRHRQDAL;RRHRQDALP;

RHRQDALPGiHRQDALPGPjRQDALPGPCiQDALPGPCIiDALPGPCIAjALPGPCIAS;

LPGPCIASTjPGPCIASTPiGPCIASTPKiPCIASTPKKiCIASTPKKHilASTPKKHRjAST

PKKHRG;

10 mers:

MESRGRRCPEiESRGRRCPEMiSRGRRCPEMIiRGRRCPEMISiGRRCPEMISViRR

CPEMISVLiRCPEMISVLGiCPEMISVLGPiPEMISVLGPliEMISVLGPISiMISVLGPISG;

ISVLGPISGHiSVLGPISGHViVLGPISGHVLiLGPISGHVLKiGPISGHVLKAiPISGHVLK

AViISGHVLKAVFiSGHVLKAVFSiGHVLKAVFSRiHVLKAVFSRGiVLKAVFSRGDiLKA

VFSRGDTiKAVFSRGDTPiAVFSRGDTPViVFSRGDTPVLiFSRGDTPVLPiSRGDTPV

LPHiRGDTPVLPHEiGDTPVLPHETiDTPVLPHETRiTPVLPHETRLiPVLPHETRLLiVL

PHETRLLQiLPHETRLLQTiPHETRLLQTGiHETRLLQTGIiETRLLQTGIHiTRLLQTGIH

ViRLLQTGIHVRiLLQTGIHVRViLQTGIHVRVSiQTGIHVRVSQiTGIHVRVSQPiGIHVR

VSQPSiIHVRVSQPSLiHVRVSQPSLIiVRVSQPSLILiRVSQPSLILViVSQPSLILVSiSQ

PSLILVSQiQPSLILVSQYiPSLILVSQYTiSLILVSQYTPiLILVSQYTPDiILVSQYTPDSiL

VSQYTPDSTiVSQYTPDSTPiSQYTPDSTPCiQYTPDSTPCHiYTPDSTPCHRiTPDST

PCHRGiPDSTPCHRGDiDSTPCHRGDNiSTPCHRGDNQiTPCHRGDNQLiPCHRGD

NQLQiCHRGDNQLQViHRGDNQLQVQiRGDNQLQVQHiGDNQLQVQHTiDNQLQVQ

HTY;NQLQVQHTYF;QLQVQHTYFT;LQVQHTYFTG;QVQHTYFTGS;VQHTYFTGSE;

QHTYFTGSEViHTYFTGSEVEiTYFTGSEVENiYFTGSEVENViFTGSEVENVSiTGSE

VENVSViGSEVENVSVNiSEVENVSVNViEVENVSVNVHiVENVSVNVHNiENVSVNV

HNPiNVSVNVHNPTiVSVNVHNPTGiSVNVHNPTGRiVNVHNPTGRSiNVHNPTGRSI;

VHNPTGRSICiHNPTGRSICPiNPTGRSICPSiPTGRSICPSQiTGRSICPSQEiGRSICP

SQEPiRSICPSQEPMiSICPSQEPMSilCPSQEPMSIiCPSQEPMSIYiPSQEPMSIYViS

QEPMSIYVYiQEPMSIYVYAiEPMSIYVYALiPMSIYVYALPiMSIYVYALPLiSIYVYALPL

KiIYVYALPLKMiYVYALPLKMLiVYALPLKMLNiYALPLKMLNIiALPLKMLNIPiLPLKML

NIPSiPLKMLNIPSIiLKMLNIPSINiKMLNIPSINViMLNIPSINVHiLN IPSINVHHiNIPSINV

HHYiIPSINVHHYPiPSINVHHYPSiSINVHHYPSAiINVHHYPSAAiNVHHYPSAAEiVHH

YPSAAERiHHYPSAAERKiHYPSAAERKHiYPSAAERKHRiPSAAERKHRHiSAAERK

HRHLiAAERKHRHLPiAERKHRHLPViERKHRHLPVAiRKHRHLPVADiKHRHLPVADA

;HRHLPVADAV;RHLPVADAVI;HLPVADAVIH;LPVADAVIHA;PVADAVIHAS;VADAVI

HASGiADAVIHASGKiDAVIHASGKQiAVIHASGKQMiVIHASGKQMWiIHASGKQMW

Q;HASGKQMWQA;ASGKQMWQAR;SGKQMWQARL;GKQMWQARLT;KQMWQARL

TV;QMWQARLTVS;MWQARLTVSG;WQARLTVSGL;QARLTVSGLA;ARLTVSGLAW;

RLTVSG LAWTiLTVSG LAWTRiTVSG LAWTRQ;VSGLAWTRQQ;SG LAWTRQQN;GL

AWTRQQNQiLAWTRQQNQW ;AWTRQQNQWK;WTRQQNQWKE;TRQQNQWKEP;

RQQNQWKEPD;QQNQWKEPDV;QNQW KEPDVY;NQWKEPDVYY;QWKEPDVYYT;

WKEPDVYYTSiKEPDVYYTSAiEPDVYYTSAFiPDVYYTSAFViDVYYTSAFVFiVYYTS

AFVFPiYYTSAFVFPTiYTSAFVFPTKiTSAFVFPTKDiSAFVFPTKDViAFVFPTKDVAiF

VFPTKDVALiVFPTKDVALRiFPTKDVALRHiPTKDVALRHViTKDVALRHVViKDVALR

HVVCiDVALRHVVCAiVALRHVVCAHiALRHVVCAHEiLRHVVCAHELiRHVVCAHELV

;HVVCAHELVC;VVCAHELVCS;VCAHELVCSM;CAHELVCSME;AHELVCSMEN;HEL

VCSMENTiELVCSMENTRiLVCSMENTRAiVCSMENTRATiCSMENTRATKiSMENTR

ATKM;MENTRATKMQ;ENTRATKMQV;NTRATKMQVI;TRATKMQVIG;RATKMQVIGD

;ATKMQVIGDQ;TKMQVIGDQY;KMQVIGDQYV;MQVIGDQYVK;QVIGDQYV KViVIGD

QYVKVYiIGDQYVKVYLiGDQYVKVYLEiDQYVKVYLESiQYVKVYLESFiYVKVYLESF

CiVKVYLESFCEiKVYLESFCEDiVYLESFCEDViYLESFCEDVPiLESFCEDVPSiESFC o

EDVPSGiSFCEDVPSGKjFCEDVPSGKLiCEDVPSGKLFiEDVPSGKLFMjDVPSGKLF

MHjVPSGKLFMHViPSGKLFMHVTiSGKLFMHVTLjGKLFMHVTLGiKLFMHVTLGSiLF

MHVTLGSDiFMHVTLGSDViMHVTLGSDVEjHVTLGSDVEEiVTLGSDVEEDjTLGSDV

EEDLjLGSDVEEDLTiGSDVEEDLTMiSDVEEDLTMTiDVEEDLTMTRjVEEDLTMTRN;

EEDLTMTRNP;EDLTMTRNPQ;DLTMTRNPQP;LTMTRNPQPF;TMTRNPQPFM;MTR

NPQPFMR;TRNPQPFMRP;RNPQPFMRPH;NPQPFMRPHE;PQPFMRPHER;QPFM

RPHERNiPFMRPHERNGiFMRPHERNGFjMRPHERNGFTjRPHERNGFTViPHERNG

FTVLjHERNGFTVLCiERNGFTVLCPiRNGFTVLCPKjNGFTVLCPKNiG FTVLCPKNM;

FTVLCPKNMIjTVLCPKNMIliVLCPKNMIIKiLCPKNMIIKPjCPKNMIIKPGiPKNMIIKPG

KjKNMIIKPGKliNMIIKPGKISiMIIKPGKISHillKPGKISHlilKPGKISHIMiKPGKISHIMLjP

GKISHIMLDiGKISHIMLDViKISHIMLDVAjISHIMLDVAFiSHIMLDVAFTjHIMLDVAFTS;

IMLDVAFTSHiMLDVAFTSHEjLDVAFTSHEHjDVAFTSHEHFiVAFTSHEHFGiAFTSH

EHFGLiFTSHEHFGLLjTSHEHFGLLCiSHEHFGLLCPiHEHFGLLCPKjEHFGLLCPKS;

HFGLLCPKSIjFGLLCPKSIPiGLLCPKSIPGiLLCPKSIPGLjLCPKSIPGLSiCPKSIPGLS

IjPKSIPGLSISiKSIPGLSISGiSIPGLSISGNilPGLSISGNLjPGLSISGNLLiGLSISGNLL

MiLSISGNLLMNiSISGNLLMNGiISGNLLMNGQiSGNLLMNGQQiGNLLMNGQQIjNLL

MNGQQIFiLLMNGQQIFLjLMNGQQIFLEjMNGQQIFLEViNGQQIFLEVQiGQQIFLEV

QAjQQIFLEVQAliQIFLEVQAIRilFLEVQAIREiFLEVQAIRETjLEVQAIRETViEVQAIRE

TVEjVQAIRETVELiQAIRETVELRjAIRETVELRQilRETVELRQYiRETVELRQYDiETV

ELRQYDPiTVELRQYDPViVELRQYDPVAjELRQYDPVAAiLRQYDPVAALjRQYDPVA

ALFjQYDPVAALFFiYDPVAALFFFiDPVAALFFFDiPVAALFFFDIΛ/AALFFFDIDjAALF

FFDIDLiALFFFDIDLLiLFFFDIDLLLiFFFDIDLLLQiFFDIDLLLQRiFDIDLLLQRGiDIDLL

LQRGPiIDLLLQRGPQiDLLLQRGPQYiLLLQRGPQYSiLLQRGPQYSEiLQRGPQYSE

HiQRGPQYSEHPiRGPQYSEHPTiGPQYSEHPTFiPQYSEHPTFTiQYSEHPTFTSiYS

EHPTFTSQiSEHPTFTSQYiEHPTFTSQYRiHPTFTSQYRIiPTFTSQYRIQiTFTSQYRI

QGiFTSQYRIQGKiTSQYRIQGKLiSQYRIQGKLEiQYRIQGKLEYiYRIQGKLEYRiRIQ

GKLEYRH;IQGKLEYRHT;QGKLEYRHTW;GKLEYRHTWD;KLEYRHTWDR;LEYRHT

WDRH;EYRHTWDRHD;YRHTWDRHDE;RHTWDRHDEG;HTWDRHDEGA;TWDRHD

EGAA;WDRHDEGAAQ;DRHDEGAAQG;RHDEGAAQGD;HDEGAAQGDD;DEGAAQ

GDDD;EGAAQGDDDV;GAAQGDDDVW;AAQGDDDVWT;AQGDDDVWTS;QGDDDV

WTSG;GDDDVWTSGS;DDDVWTSGSD;DDVWTSGSDS;DVWTSGSDSD;VWTSGS

DSDE;WTSGSDSDEE;TSGSDSDEEL;SGSDSDEELV;GSDSDEELVT;SDSDEELVTT

;DSDEELVTTE;SDEELVTTER;DEELVTTERK;EELVTTERKT;ELVTTERKTP;LVTTER

KTPR;VTTERKTPRV;TTERKTPRVT;TERKTPRVTG;ERKTPRVTGG;RKTPRVTGGG;

KTPRVTGGGAiTPRVTGGGAMiPRVTGGGAMAiRVTGGGAMASiVTGGGAMASAiT

GGGAMASASiGGGAMASASTiGGAMASASTSiGAMASASTSAiAMASASTSAGiMAS

ASTSAGRiASASTSAGRKiSASTSAGRKRiASTSAGRKRKiSTSAGRKRKSiTSAGRK

RKSAiSAGRKRKSASiAGRKRKSASSiGRKRKSASSAiRKRKSASSATiKRKSASSAT

AiRKSASSATACiKSASSATACTiSASSATACTAiASSAT ACTAG ;SSATACTAGV;SAT

ACTAGVMiATACTAGVMTiTACTAGVMTRiACTAGVMTRGiCTAGVMTRGRiTAGVM

TRGRLiAGVMTRGRLKiGVMTRGRLKAiVMTRGRLKAEiMTRGRLKAESiTRGRLKAE

STiRGRLKAESTViGRLKAESTVAiRLKAESTVAPiLKAESTVAPEiKAESTVAPEEiAES

TVAPEEDiESTVAPEEDTiSTVAPEEDTDiTVAPEEDTDEiVAPEEDTDEDiAPEEDTD

EDSiPEEDTDEDSDiEEDTDEDSDNiEDTDEDSDNEiDTDEDSDNEIiTDEDSDNEIHiD

EDSDNEIHNiEDSDNEIHNPiDSDNEIHNPAiSDNEIHNPAViDNEIHNPAVFiNEIHNPA

VFTiEIHNPAVFTWiIHNPAVFTWPiHNPAVFTWPPiNPAVFTWPPWiPAVFTWPPWQ;

AVFTWPPWQAiVFTWPPWQAGiFTWPPWQAGIiTWPPWQAGILiWPPWQAGILAiPP

WQAGILARiPWQAGILARNiWQAGILARNLiQAGILARNLViAGILARNLVPiGILARNLV

PMiILARNLVPMViLARNLVPMVAiARNLVPMVATiRNLVPMVATViNLVPMVATVQiLV

PMVATVQG;VPMVATVQGQ;PMVATVQGQN;MVATVQGQNL;VATVQGQNLK;ATVQ

GQNLKY ;TVQGQNLKYQ;VQGQNLKYQE;QGQNLKYQEF;GQNLKYQEFF;QNLKYQ

EFFW;NLKYQEFFWD;LKYQEFFWDA;KYQEFFWDAN;YQEFFWDAND;QEFFWDAN

DIiEFFWDANDIYiFFWDANDIYRiFWDANDIYRIiWDANDIYRIFiDANDIYRIFAiANDIY

RIFAEiNDIYRIFAELiDIYRIFAELEiIYRIFAELEGiYRIFAELEGViRIFAELEGVWiIFAEL

EGVWQ;FAELEGVWQP;AELEGVWQPA;ELEGVWQPAA;LEGVWQPAAQ;EGVWQP

AAQP;G VWQPAAQPK;VWQPAAQPKR;WQPAAQPKRR;QPAAQPKRRR;PAAQPKR

RRH;AAQPKRRRHR;AQPKRRRHRQ;QPKRRRHRQD;PKRRRHRQDA;KRRRHRQD

ALjRRRHRQDALPiRRHRQDALPGiRHRQDALPGPiHRQDALPGPCiRQDALPGPCI; QDALPG PCIAiDALPGPCIASiALPGPCIASTiLPGPCIASTPiPGPCIASTPKiGPCIAST PKKiPCIASTPKKHiCIASTPKKHRiIASTPKKHRG;

1 1 mers:

MESRGRRCPEMiESRGRRCPEMIiSRGRRCPEMISiRGRRCPEMISViGRRCPEMIS

VLiRRCPEMISVLGiRCPEMISVLGPiCPEMISVLGPliPEMISVLGPISiEMISVLGPISG;

MISVLGPISGHiISVLGPISGHViSVLGPISGHVLiVLGPISGHVLKiLGPISGHVLKAiGPI

SGHVLKAViPISGHVLKAVFiISGHVLKAVFSiSGHVLKAVFSRiGHVLKAVFSRGiHVL

KAVFSRGDiVLKAVFSRGDTiLKAVFSRGDTPiKAVFSRGDTPViAVFSRGDTPVLiVF

SRGDTPVLPiFSRGDTPVLPHiSRGDTPVLPHEiRGDTPVLPHETiGDTPVLPHETRiD

TPVLPHETRLiTPVLPHETRLLiPVLPHETRLLQiVLPHETRLLQTiLPHETRLLQTGiPH

ETRLLQTGIiHETRLLQTGIHiETRLLQTGIHViTRLLQTGIHVRiRLLQTGIHVRViLLQT

GIHVRVSiLQTGIHVRVSQiQTGIHVRVSQPiTGIHVRVSQPSiGIHVRVSQPSLiIHVRV

SQPSLIiHVRVSQPSLILiVRVSQPSLILViRVSQPSLILVSiVSQPSLILVSQiSQPSLILV

SQYiQPSLILVSQYTiPSLILVSQYTPiSLILVSQYTPDiLILVSQYTPDSiILVSQYTPDST;

LVSQYTPDSTPiVSQYTPDSTPCiSQYTPDSTPCHiQYTPDSTPCHRiYTPDSTPCHR

GiTPDSTPCHRGDiPDSTPCHRGDNiDSTPCHRGDNQiSTPCHRGDNQLiTPCHRGD

NQLQiPCHRGDNQLQViCHRGDNQLQVQiHRGDNQLQVQHiRGDNQLQVQHTiGDN

QLQVQHTYiDNQLQVQHTYFiNQLQVQHTYFTiQLQVQHTYFTGiLQVQHTYFTGSiQ

VQHTYFTGSE;VQHTYFTGSEV;QHTYFTGSEVE;HTYFTGSEVEN;TYFTGSEVENV;

YFTGSEVENVSiFTGSEVENVSViTGSEVENVSVNiGSEVENVSVNViSEVENVSVNV

HiEVENVSVNVHNiVENVSVNVHNPiENVSVNVHNPTiNVSVNVHNPTGiVSVNVHNP

TGRiSVNVHNPTGRSiVNVHNPTGRSIiNVHNPTGRSICiVHNPTGRSICPiHNPTGRSI

CPSiNPTGRSICPSQiPTGRSICPSQEiTGRSICPSQEPiGRSICPSQEPMiRSICPSQE

PMSiSICPSQEPMSIiICPSQEPMSIYiCPSQEPMSIYViPSQEPMSIYVYiSQEPMSIYV

YAiQEPMSIYVYALiEPMSIYVYALPiPMSIYVYALPLiMSIYVYALPLKiSIYVYALPLKMiI

YVYALPLKMLiYVYALPLKMLNiVYALPLKMLNIiYALPLKMLNIPiALPLKMLNIPSiLPLK

MLNIPSIiPLKMLNIPSINiLKMLNIPSINViKMLNIPSINVHiMLNIPSINVHHiLNIPSINVH

HYiNIPSINVHHYPiIPSINVHHYPSiPSINVHHYPSAiSINVHHYPSAAiINVHHYPSAAE;

NVHHYPSAAERiVHHYPSAAERKiHHYPSAAERKHiHYPSAAERKHRiYPSAAERKHR

H;PSAAERKHRHL;SAAERKHRHLP;AAERKHRHLPV;AERKHRHLPVA;ERKHRHLPV

ADiRKHRHLPVADAiKHRHLPVADAViHRHLPVADAVIiRHLPVADAVIHiHLPVADAVI

HAiLPVADAVIHASiPVADAVIHASGiVADAVIHASGKiADAVIHASGKQiDAVIHASGKQ

M;AVIHASGKQMW;VIHASGKQMWQ;IHASGKQMWQA;HASGKQMWQAR;ASGKQM

WQARL;SGKQMWQARLT;GKQMWQARLTV;KQMWQARLTVS;QMWQARLTVSG;M

WQARLTVSGL;WQARLTVSGLA;QARLTVSGLAW;ARLTVSGLAWT;RLTVSGLAWT

R;LTVSGLAWTRQ;TVSG LAWTRQQ;VSGLAWTRQQN;SGLAWTRQQNQ;GLAWTR

QQNQW;LAWTRQQNQWK;AWTRQQNQWKE;WTRQQNQWKEP;TRQQNQWKEPD

;RQQNQWKEPDV;QQNQWKEPDVY;QNQW KEPDVYY;NQWKEPDVYYT;QW KEPD

VYYTS;WKEPDVYYTSA;KEPDVYYTSAF;EPDVYYTSAFV;PDVYYTSAFVF;DVYYTS

AFVFPiVYYTSAFVFPTiYYTSAFVFPTKiYTSAFVFPTKDiTSAFVFPTKDViSAFVFPT

KDVAiAFVFPTKDVALiFVFPTKDVALRiVFPTKDVALRHiFPTKDVALRHViPTKDVAL

RHVViTKDVALRHVVCiKDVALRHVVCAiDVALRHVVCAHiVALRHVVCAHEiALRHVV

CAHELiLRHVVCAHELViRHVVCAHELVCiHVVCAHELVCSiVVCAHELVCSMiVCAHE

LVCSME;CAHELVCSMEN;AHELVCSMENT;HELVCSMENTR;ELVCSMENTRA;LVC

SMENTRATiVCSMENTRATKiCSMENTRATKMiSMENTRATKMQiMENTRATKMQV;

ENTRATKMQVIiNTRATKMQVIGiTRATKMQVIGDiRATKMQVIGDQiATKMQVIGDQY

;TKMQVIGDQYV;KMQVIGDQYVK;MQVIGDQYVKV;QVIGDQYVKVY;VIGDQYVKVY

LiIGDQYVKVYLEiGDQYVKVYLESiDQYVKVYLESFiQYVKVYLESFCiYVKVYLESFC

EiVKVYLESFCEDiKVYLESFCEDViVYLESFCEDVPiYLESFCEDVPSiLESFCEDVPS

GiESFCEDVPSGKiSFCEDVPSGKLiFCEDVPSGKLFiCEDVPSGKLFMiEDVPSGKLF

MH;DVPSGKLFMHV;VPSGKLFMHVT;PSGKLFMHVTL;SGKLFMHVTLG;GKLFMHV

TLGS;KLFMHVTLGSD;LFMHVTLGSDV;FMHVTLGSDVE;MHVTLGSDVEE;HVTLGS

DVEED;VTLGSDVEEDL;TLGSDVEEDLT;LGSDVEEDLTM;GSDVEEDLTMT;SDVEE

DLTMTRiDVEEDLTMTRNiVEEDLTMTRNPiEEDLTMTRNPQiEDLTMTRNPQPiDLT

MTRNPQPF;LTMTRNPQPFM;TMTRNPQPFMR;MTRNPQPFMRP;TRNPQPFMRPH;

RNPQPFMRPHE;NPQPFMRPHER;PQPFMRPHERN;QPFMRPHERNG;PFMRPHER

NGFiFMRPHERNGFTjMRPHERNGFTViRPHERNGFTVLiPHERNGFTVLCiHERNGF TVLCPiERNGFTVLCPKiRNGFTVLCPKNiNGFTVLCPKNMiGFTVLCPKNMIiFTVLCP

KNMIIiTVLCPKNMIIKiVLCPKNMIIKPiLCPKNMIIKPGiCPKNMIIKPGKiPKNMIIKPGKI;

KNMIIKPGKISiNMIIKPGKISHiMIIKPGKISHIiIIKPGKISHIMiIKPGKISHIMLiKPGKISHI

MLDiPGKISHIMLDViGKISHIMLDVAiKISHIMLDVAFiISHIMLDVAFTiSHIMLDVAFTS;

HIMLDVAFTSHiIMLDVAFTSHEiMLDVAFTSHEHiLDVAFTSHEHFiDVAFTSHEHFG;

VAFTSHEHFGLiAFTSHEHFGLLiFTSHEHFGLLCiTSHEHFGLLCPiSHEHFGLLCPK;

HEHFGLLCPKSiEHFGLLCPKSIiHFGLLCPKSIPiFGLLCPKSIPGiGLLCPKSIPGLiLL

CPKSIPGLSiLCPKSIPGLSIiCPKSIPGLSISiPKSIPGLSISGiKSIPG LSISGNiSIPG LSIS

GNLiIPGLSISGNLLiPGLSISGNLLMiGLSISGNLLMNiLSISGNLLMNGiSISGNLLMNG

Q;ISGNLLMNGQQ;SGNLLMNGQQI;GNLLMNGQQIF;NLLMNGQQIFL;LLMNGQQIFL

EiLMNGQQIFLEViMNGQQIFLEVQiNGQQIFLEVQAiGQQIFLEVQAIiQQIFLEVQAIR;

QIFLEVQAIREiIFLEVQAIRETiFLEVQAIRETViLEVQAIRETVEiEVQAIRETVELiVQAI

RETVELRiQAIRETVELRQiAIRETVELRQYiIRETVELRQYDiRETVELRQYDPiETVEL

RQYDPV;TVELRQYDPVA;VELRQYDPVAA;ELRQYDPVAAL;LRQYDPVAALF;RQYD

PVAALFFiQYDPVAALFFFiYDPVAALFFFDiDPVAALFFFDIiPVAALFFFDIDiVAALFF

FDIDLiAALFFFDIDLLiALFFFDIDLLLiLFFFDIDLLLQiFFFDIDLLLQRiFFDIDLLLQRG;

FDIDLLLQRGPiDIDLLLQRGPQiIDLLLQRGPQYiDLLLQRGPQYSiLLLQRGPQYSEiL

LQRGPQYSEH;LQRGPQYSEHP;QRGPQYSEHPT;RGPQYSEHPTF;GPQYSEHPTF

T;PQYSEHPTFTS;QYSEHPTFTSQ;YSEHPTFTSQY;SEHPTFTSQYR;EHPTFTSQY

RIiHPTFTSQYRIQiPTFTSQYRIQGiTFTSQYRIQGKiFTSQYRIQGKLiTSQYRIQGKLE

;SQYRIQGKLEY;QYRIQGKLEYR;YRIQGKLEYRH;RIQG KLEYRHTiIQGKLEYRHTW;

QGKLEYRHTWD;GKLEYRHTWDR;KLEYRHTWDRH;LEYRHTWDRHD;EYRHTWDR

HDE;YRHTWDRHDEG;RHTWDRHDEGA;HTWDRHDEGAA;TWDRHDEGAAQ;WDR

HDEGAAQG;DRHDEGAAQGD;RHDEGAAQGDD;HDEGAAQGDDD;DEGAAQGDDD

V;EGAAQGDDDVW;GAAQGDDDVWT;AAQGDDDVWTS;AQGDDDVWTSG;QGDDD

VWTSGS;GDDDVWTSGSD;DDDVWTSGSDS;DDVWTSGSDSD;DVWTSGSDSDE;V

WTSGSDSDEE;WTSGSDSDEEL;TSGSDSDEELV;SGSDSDEELVT;GSDSDEELVTT

;SDSDEELVTTE;DSDEELVTTER;SDEELVTTERK;DEELVTTERKT;EELVTTERKTP;

ELVTTERKTPR;LVTTERKTPRV;VTTERKTPRVT;TTERKTPRVTG;TERKTPRVTGG;

ERKTPRVTGGG;RKTPRVTGGGA;KTPRVTGGGAM;TPRVTGGGAMA;PRVTGGGA

MASiRVTGGGAMASAiVTGGGAMASASiTGGGAMASASTiGGGAMASASTSiGGAM

ASASTSAiGAMASASTSAGiAMASASTSAGRiMASASTSAGRKiASASTSAGRKRiSA

STSAGRKRKiASTSAGRKRKSiSTSAGRKRKSAiTSAGRKRKSASiSAGRKRKSASS;

AGRKRKSASSAiGRKRKSASSATiRKRKSASSATAiKRKSASSATACiRKSASSATAC

TiKSASSATACTAiSASSATACTAGiASSATACTAGViSSATACTAGVMiSATACTAGV

MTiATACTAGVMTRiTACTAGVMTRGiACTAGVMTRGRiCTAGVMTRGRLiTAGVMT

RGRLK;AGVMTRGRLKA;GVMTRGRLKAE;VMTRGRLKAES;MTRGRLKAEST;TRGR

LKAESTViRGRLKAESTVAiGRLKAESTVAPiRLKAESTVAPEiLKAESTVAPEEiKAES

TVAPEEDiAESTVAPEEDTiESTVAPEEDTDiSTVAPEEDTDEiTVAPEEDTDEDiVAPE

EDTDEDSiAPEEDTDEDSDiPEEDTDEDSDNiEEDTDEDSDNEiEDTDEDSDNEIiDTD

EDSDNEIHiTDEDSDNEIHNiDEDSDNEIHNPiEDSDNEIHNPAiDSDNEIHNPAViSDN

EIHNPAVFiDNEIHNPAVFTiNEIHNPAVFTWiEIHNPAVFTWPiIHNPAVFTWPPiHNPA

VFTWPPW;NPAVFTWPPWQ;PAVFTWPPWQA;AVFTWPPWQAG;VFTWPPWQAGI;

FTWPPWQAGIL;TWPPWQAGILA;WPPWQAGILAR;PPWQAGILARN;PWQAGILARN

LiWQAGILARNLViQAGILARNLVPiAGILARNLVPMiGILARNLVPMViILARNLVPMVA;

LARNLVPMVAT ;ARNLVPMVATV;RNLVPMVATVQ;NLVPMVATVQG;LVPMVATVQG

Q;VPMVATVQGQN;PMVATVQGQN L;MVATVQGQN LK;VATVQGQN LKY;ATVQGQN

LKYQ;TVQGQNLKYQE;VQGQNLKYQEF;QGQNLKYQEFF;GQNLKYQEFFW;QNLK

YQEFFWDiNLKYQEFFWDAiLKYQEFFWDANiKYQEFFWDANDiYQEFFWDANDIiQ

EFFWDANDIYiEFFWDANDIYRiFFWDANDIYRIiFWDANDIYRIFiWDANDIYRIFAiDA

NDIYRIFAEiANDIYRIFAELiNDIYRIFAELEiDIYRIFAELEGiIYRIFAELEGViYRIFAELE

GVW;RIFAELEGVWQ;IFAELEGVWQP;FAELEGVWQPA;AELEGVWQPAA;ELEGVW

QPAAQ;LEGVWQPAAQP;EGVWQPAAQPK;GVWQPAAQPKR;VWQPAAQPKRR;W

QPAAQPKRRR;QPAAQPKRRRH;PAAQPKRRRHR;AAQPKRRRHRQ;AQPKRRRHR

QD;QPKRRRHRQDA;PKRRRHRQDAL;KRRRHRQDALP;RRRHRQDALPG;RRHRQ

DALPGPiRHRQDALPGPCiHRQDALPGPCIiRQDALPGPCIAiQDALPGPCIASiDALP

GPCIASTiALPGPCIASTPiLPGPCIASTPKiPGPCIASTPKKiGPCIASTPKKHiPCIASTP

KKHRjCIASTPKKHRG; 13 mers:

MESRGRRCPEMISiESRGRRCPEMISViSRGRRCPEMISVLjRGRRCPEMISVLGiGR

RCPEMISVLGPiRRCPEMISVLGPliRCPEMISVLGPISiCPEMISVLGPISGiPEMISVLG

PISGHiEMISVLGPISGHViMISVLGPISGHVLiISVLGPISGHVLKiSVLGPISGHVLKAiV

LGPISGHVLKAViLGPISGHVLKAVFiGPISGHVLKAVFSiPISGHVLKAVFSRiISGHVLK

AVFSRGiSGHVLKAVFSRGDiGHVLKAVFSRGDTiHVLKAVFSRGDTPiVLKAVFSRG

DTPViLKAVFSRGDTPVLiKAVFSRGDTPVLPiAVFSRGDTPVLPHiVFSRGDTPVLPH

EiFSRGDTPVLPHETiSRGDTPVLPHETRiRGDTPVLPHETRLiGDTPVLPHETRLLiDT

PVLPHETRLLQiTPVLPHETRLLQTiPVLPHETRLLQTGiVLPHETRLLQTGIiLPHETRL

LQTGIHiPHETRLLQTGIHViHETRLLQTGIHVRiETRLLQTGIHVRViTRLLQTGIHVRV

SiRLLQTGIHVRVSQiLLQTGIHVRVSQPiLQTGIHVRVSQPSiQTGIHVRVSQPSLiTGI

HVRVSQPSLIiGIHVRVSQPSLILiIHVRVSQPSLILViHVRVSQPSLILVSiVRVSQPSLIL

VSQiRVSQPSLILVSQYiVSQPSLILVSQYTiSQPSLILVSQYTPiQPSLILVSQYTPDiPS

LILVSQYTPDSiSLILVSQYTPDSTiLILVSQYTPDSTPiILVSQYTPDSTPCiLVSQYTPD

STPCHiVSQYTPDSTPCHRiSQYTPDSTPCHRGiQYTPDSTPCHRGDiYTPDSTPCH

RGDNiTPDSTPCHRGDNQiPDSTPCHRGDNQLiDSTPCHRGDNQLQiSTPCHRGDN

QLQViTPCHRGDNQLQVQiPCHRGDNQLQVQHiCHRGDNQLQVQHTiHRGDNQLQ

VQHTY;RGDNQLQVQHTYF;GDNQLQVQHTYFT;DNQLQVQHTYFTG;NQLQVQHTY

FTGS;QLQVQHTYFTGSE;LQVQHTYFTGSEV;QVQHTYFTGSEVE;VQHTYFTGSEV

ENiQHTYFTGSEVENViHTYFTGSEVENVSiTYFTGSEVENVSViYFTGSEVENVSVN;

FTGSEVENVSVNViTGSEVENVSVNVHiGSEVENVSVNVHNiSEVENVSVNVHNPiEV

ENVSVNVHNPTiVENVSVNVHNPTGiENVSVNVHNPTGRiNVSVNVHNPTGRSiVSV

NVHNPTGRSIiSVNVHNPTGRSICiVNVHNPTGRSICPiNVHNPTGRSICPSiVHNPTG

RSICPSQiHNPTGRSICPSQEiNPTGRSICPSQEPiPTGRSICPSQEPMiTGRSICPSQ

EPMSiGRSICPSQEPMSIiRSICPSQEPMSIYiSICPSQEPMSIYViICPSQEPMSIYVYiC

PSQEPMSIYVYAiPSQEPMSIYVYALiSQEPMSIYVYALPiQEPMSIYVYALPLiEPMSIY

VYALPLKiPMSIYVYALPLKMiMSIYVYALPLKMLiSIYVYALPLKMLNiIYVYALPLKMLN

IiYVYALPLKMLNIPiVYALPLKMLNIPSiYALPLKMLN IPSIiALPLKMLNIPSINiLPLKML

NIPSINViPLKMLNIPSINVHiLKMLNIPSINVHHiKMLN IPSINVHHYiMLNIPSINVHHYP;

LNIPSINVHHYPSiNIPSINVHHYPSAiIPSINVHHYPSAAiPSINVHHYPSAAEiSINVHH

YPSAAERiINVHHYPSAAERKiNVHHYPSAAERKHiVHHYPSAAERKHRiHHYPSAAE

RKHRHiHYPSAAERKHRHLiYPSAAERKHRHLPiPSAAERKHRHLPViSAAERKHRHL

PVAiAAERKHRHLPVADiAERKHRHLPVADAiERKHRHLPVADAViRKHRHLPVADAVI

;KHRHLPVADAVIH;HRHLPVADAVIHA;RHLPVADAVIHAS;HLPVADAVIHASG;LPVA

DAVIHASGKiPVADAVIHASGKQiVADAVIHASGKQMiADAVIHASGKQMWiDAVIHAS

GKQMWQ;AVIHASGKQMWQA;VIHASGKQMWQAR;IHASGKQMWQARL;HASGKQ

MWQARLT ;ASGKQMWQARLTV;SGKQMWQARLTVS;GKQMWQARLTVSG;KQMW

QARLTVSGLiQMWQARLTVSGLAiMWQARLTVSGLAWiWQARLTVSGLAWTiQARL

TVSG LAWTRiARLTVSG LAWTRQ;RLTVSGLAWTRQQ;LTVSG LAWTRQQN ;TVSGL

AWTRQQNQ;VSG LAWTRQQNQW;SG LAWTRQQNQWK;G LAWTRQQNQWKE;LAW

TRQQNQWKEP;AWTRQQNQWKEPD;WTRQQNQWKEPDV;TRQQNQWKEPDVY;R

QQNQWKEPDVYY;QQNQWKEPDVYYT;QNQWKEPDVYYTS;NQW KEPDVYYTSA;

QWKEPDVYYTSAFiWKEPDVYYTSAFViKEPDVYYTSAFVFiEPDVYYTSAFVFPiPD

VYYTSAFVFPTiDVYYTSAFVFPTKiVYYTSAFVFPTKDiYYTSAFVFPTKDViYTSAFV

FPTKDVAiTSAFVFPTKDVALiSAFVFPTKDVALRiAFVFPTKDVALRHiFVFPTKDVAL

RHViVFPTKDVALRHVViFPTKDVALRHVVCiPTKDVALRHVVCAiTKDVALRHVVCAH

;KDVALRHVVCAHE;DVALRHVVCAHEL;VALRHVVCAHELV;ALRHVVCAHELVC;LR

HVVCAHELVCSiRHVVCAHELVCSMiHVVCAHELVCSMEiVVCAHELVCSMENiVCA

HELVCSMENT;CAHELVCSMENTR;AHELVCSMENTRA;HELVCSMENTRAT;ELVCS

MENTRATKiLVCSMENTRATKMiVCSMENTRATKMQiCSMENTRATKMQViSMENTR

ATKMQVIiMENTRATKMQVIGiENTRATKMQVIGDiNTRATKMQVIGDQiTRATKMQVI

GDQYiRATKMQVIGDQYViATKMQVIGDQYVKiTKMQVIGDQYVKViKMQVIGDQYVK

VYiMQVIGDQYVKVYLiQVIGDQYVKVYLEiVIGDQYVKVYLESiIGDQYVKVYLESFiG

DQYVKVYLESFCiDQYVKVYLESFCEiQYVKVYLESFCEDiYVKVYLESFCEDViVKVY

LESFCEDVPiKVYLESFCEDVPSiVYLESFCEDVPSGiYLESFCEDVPSGKiLESFCED

VPSGKLiESFCEDVPSGKLFiSFCEDVPSGKLFMiFCEDVPSGKLFMHiCEDVPSGKL

FMHV;EDVPSGKLFMHVT;DVPSGKLFMHVTL;VPSGKLFMHVTLG;PSGKLFMHVTL GSiSGKLFMHVTLGSDjGKLFMHVTLGSDVjKLFMHVTLGSDVEjLFMHVTLGSDVEE;

FMH\πXGSDVEED;MHVTLGSDVEEDL;HVTLGSDVEEDLT;VTLGSDVEEDLTM;TLG

SDVEEDLTMTjLGSDVEEDLTMTRiGSDVEEDLTMTRNjSDVEEDLTMTRNPjDVEED

LTMTRNPQiVEEDLTMTRNPQPjEEDLTMTRNPQPFjEDLTMTRNPQPFMjDLTMTRN

PQPFMRiLTMTRNPQPFMRPjTMTRNPQPFMRPHjMTRNPQPFMRPHEjTRNPQPF

MRPHERjRNPQPFMRPHERNjNPQPFMRPHERNGiPQPFMRPHERNGFjQPFMRPH

ERNGFTiPFMRPHERNGFTVjFMRPHERNGFTVLjMRPHERNGFTVLCjRPHERNGF

TVLCPiPHERNGFTVLCPKiHERNGFTVLCPKNjERNGFTVLCPKNMjRNGFTVLCPK

NMIjNGFTVLCPKNMIliGFTVLCPKNMIIKiFTVLCPKNMIIKPjTVLCPKNMIIKPGiVLCP

KNMIIKPGKiLCPKNMIIKPGKliCPKNMIIKPGKISiPKNMIIKPGKISHjKNMIIKPGKISHI;

NMIIKPGKISHIMjMIIKPGKISHIMLillKPGKISHIMLDilKPGKISHIMLDVjKPGKISHIML

DVAjPGKISH IMLDVAFiGKISHIMLDVAFTjKISHIMLDVAFTSilSHIMLDVAFTSHiSHIM

LDVAFTSHEjHIMLDVAFTSHEHjIMLDVAFTSHEHFjMLDVAFTSHEHFGiLDVAFTSH

EHFGLjDVAFTSHEHFGLLjVAFTSHEHFGLLCiAFTSHEHFGLLCPiFTSHEHFGLLCP

KjTSHEHFGLLCPKSiSHEHFGLLCPKSIjHEHFGLLCPKSIPiEHFGLLCPKSIPGiHFG

LLCPKSIPGLjFGLLCPKSIPGLSiGLLCPKSIPGLSIjLLCPKSIPGLSISiLCPKSIPGLSIS

GiCPKSIPGLSISGNiPKSIPGLSISGNLjKSIPGLSISGNLLiSIPGLSISGNLLMjlPGLSIS

GNLLMNjPGLSISGNLLMNGjGLSISGNLLMNGQjLSISGNLLMNGQQiSISGNLLMNG

QQlilSGNLLMNGQQIFiSGNLLMNGQQIFLjGNLLMNGQQIFLEiNLLMNGQQIFLEVjL

LMNGQQIFLEVQiLMNGQQIFLEVQAjMNGQQIFLEVQAIjNGQQIFLEVQAIRjGQQIF

LEVQAIREiQQIFLEVQAIRETjQIFLEVQAIRETVjIFLEVQAIRETVEjFLEVQAIRETVEL iLEVQAIRETVELRjEVQAIRETVELRQiVQAIRETVELRQYiQAIRETVELRQYDjAIRET

VELRQYDPilRETVELRQYDPVjRETVELRQYDPVAjETVELRQYDPVAAjTVELRQYD

PVAALiVELRQYDPVAALFjELRQYDPVAALFFjLRQYDPVAALFFFjRQYDPVAALFFF

DiQYDPVAALFFFDIiYDPVAALFFFDIDjDPVAALFFFDIDLiPVAALFFFDIDLLjVAALF

FFDIDLLLjAALFFFDIDLLLQjALFFFDIDLLLQRjLFFFDIDLLLQRGiFFFDIDLLLQRGP;

FFDIDLLLQRGPQiFDIDLLLQRGPQYjDIDLLLQRGPQYSjIDLLLQRGPQYSEjDLLLQ

RGPQYSEHjLLLQRGPQYSEHPjLLQRGPQYSEHPTjLQRGPQYSEHPTFiQRGPQY

SEHPTFT;RGPQYSEHPTFTS;GPQYSEHPTFTSQ;PQYSEHPTFTSQY;Q YSEHPTF

TSQYRjYSEHPTFTSQYRIjSEHPTFTSQYRIQiEHPTFTSQYRIQGjHPTFTSQYRIQG

KiPTFTSQYRIQGKLiTFTSQYRIQGKLEjFTSQYRIQGKLEYjTSQYRIQGKLEYRiSQY

RIQGKLEYRHiQYRIQGKLEYRHTjYRIQGKLEYRHTWjRIQGKLEYRHTWDjlQGKLE

YRHTWDR;QGKLEYRHTWDRH;GKLEYRHTWDRHD;KLEYRHTWDRHDE;LEYRHT

WDRHDEG;EYRHTWDRHDEGA;YRHTWDRHDEGAA;RHTWDRHDEGAAQ;HTWD

RHDEGAAQG;TWDRHDEGAAQGD;WDRHDEGAAQGDD;DRHDEGAAQGDDD;RH

DEGAAQGDDDV;HDEGAAQGDDDVW;DEGAAQGDDDVWT;EGAAQGDDDVWTS;

GAAQGDDDVWTSG ;AAQGDDDVWTSGS;AQGDDDVWTSGSD;QGDDDVWTSGSD

SiGDDDVWTSGSDSDiDDDVWTSGSDSDEiDDVWTSGSDSDEEjDVWTSGSDSDE

EL;VWTSGSDSDEELV;WTSGSDSDEELVT;TSGSDSDEELVTT;SGSDSDEELVTTE;

GSDSDEELVTTERiSDSDEELVTTERKjDSDEELVTTERKTjSDEELVTTERKTPjDEEL

VTTERKTPR;EELVTTERKTPRV;ELVTTERKTPRVT;LVTTERKTPRVTG;VTTERKTP

RVTGG;TTERKTPRVTGGG;TERKTPRVTGGGA;ERKTPRVTGGGAM;RKTPRVTGG

GAMAjKTPRVTGGGAMASiTPRVTGGGAMASAjPRVTGGGAMASASjRVTGGGAMA

SAST;VTGGG AM ASASTS ;TGGG AM ASASTSA;GGG AM ASASTSAG ;GG AM ASASTS

AGRiGAMASASTSAGRKjAMASASTSAGRKRjMASASTSAGRKRKjASASTSAGRKR

KSiSASTSAGRKRKSAjASTSAGRKRKSASjSTSAGRKRKSASSjTSAGRKRKSASSA;

SAGRKRKSASSATjAGRKRKSASSATAiGRKRKSASSATACiRKRKSASSATACTjKR

KSASSAT ACTAjRKSASSATACTAGiKSASSATACTAGViSASSATACTAGVMjASSAT

ACTAGVMTiSSATACTAGVMTRjSATACTAGVMTRGiATACTAGVMTRGRjTACTAG

VMTRGRLiACTAGVMTRGRLKiCTAGVMTRGRLKAjTAGVMTRGRLKAEjAGVMTRG

RLKAESiGVMTRGRLKAESTjVMTRGRLKAESTVjMTRGRLKAESTVAjTRGRLKAES

TVAPjRGRLKAESTVAPEiGRLKAESTVAPEEjRLKAESTVAPEEDjLKAESTVAPEED

TiKAESTVAPEEDTDjAESTVAPEEDTDEjESTVAPEEDTDEDiSTVAPEEDTDEDSiTV

APEEDTDEDSDiVAPEEDTDEDSDNjAPEEDTDEDSDNEjPEEDTDEDSDNEIjEEDT

DEDSDNEIHiEDTDEDSDNEIHNjDTDEDSDNEIHNPjTDEDSDNEIHNPAjDEDSDNEI

HNPAVjEDSDNEIHNPAVFjDSDNEIHNPAVFTiSDNEIHNPAVFTWjDNEIHNPAVFT

WPjNEIHNPAVFTWPPjEIHNPAVFTWPPWjlHNPAVFTWPPWQiHNPAVFTWPPWQ

AjNPAVFTWPPWQAGiPAVFTWPPWQAGliAVFTWPPWQAGILiVFTWPPWQAGILA; FTWPPWQAGILARjTWPPWQAGILARNiWPPWQAGILARNLjPPWQAGILARNLViP

WQAG I LARN LVP;WQ AG I LARN LVPM ;Q AG I LARN LVPM V; AG I LARN LVPM VA;G I LAR

N LVPM VAT; I LARN LVPM VATV; LARN LVPM VATVQ ;ARN LVPM VATVQG ; RN LVPM VA

TVQGQ;NLVPMVATVQGQN;LVPMVATVQGQNL;VPMVATVQGQNLK;PMVATVQG

QNLKY;MVATVQGQNLKYQ;VATVQGQNLKYQE;ATVQGQNLKYQEF;TVQGQNLKY

QEFF;VQGQNLKYQEFFW;QGQNLKYQEFFWD;GQNLKYQEFFWDA;QNLKYQEFF

WDAN;NLKYQEFFWDAND;LKYQEFFWDANDI;KYQEFFWDANDIY;YQEFFWDANDI

YRiQEFFWDANDIYRhEFFWDANDIYRIFiFFWDANDIYRIFAiFWDANDIYRIFAEiWD

ANDIYRIFAELiDANDIYRIFAELEiANDIYRIFAELEGiNDIYRIFAELEGViDIYRIFAELE

GVWiIYRIFAELEGVWQiYRIFAELEGVWQPiRIFAELEGVWQPAiIFAELEGVWQPAA;

FAELEGVWQPAAQ;AELEGVWQPAAQP;ELEGVWQPAAQPK;LEGVWQPAAQPKR;

EGVWQPAAQPKRR;GVWQPAAQPKRRR;VWQPAAQPKRRRH;WQPAAQPKRRRH

R;QPAAQPKRRRHRQ;PAAQPKRRRHRQD;AAQPKRRRHRQDA;AQPKRRRHRQDA

L;QPKRRRHRQDALP;PKRRRHRQDALPG;KRRRHRQDALPGP;RRRHRQDALPGP

CiRRHRQDALPGPCIiRHRQDALPGPCIAjHRQDALPGPCIASiRQDALPGPCIASTiQD

ALPGPCIASTPiDALPGPCIASTPKiALPGPCIASTPKKiLPGPCIASTPKKHiPGPCIAST

PKKHRiGPCIASTPKKHRG;

14 mers:

MESRGRRCPEMISViESRGRRCPEMISVLiSRGRRCPEMISVLGiRGRRCPEMISVLG

PiGRRCPEMISVLGPIiRRCPEMISVLGPISiRCPEMISVLGPISGiCPEMISVLGPISGH;

PEMISVLGPISGHViEMISVLGPISGHVLiMISVLGPISGHVLKJSVLGPISGHVLKAiSVL

GPISGHVLKAViVLGPISGHVLKAVFiLGPISGHVLKAVFSiGPISGHVLKAVFSRiPISG

HVLKAVFSRGiISGHVLKAVFSRGDiSGHVLKAVFSRGDTiGHVLKAVFSRGDTPiHVL

KAVFSRGDTPViVLKAVFSRGDTPVLiLKAVFSRGDTPVLPiKAVFSRGDTPVLPHiAV

FSRGDTPVLPHE;VFSRGDTPVLPHET;FSRGDTPVLPHETR;SRGDTPVLPHETRL;R

GDTPVLPHETRLL;GDTPVLPHETRLLQ;DTPVLPHETRLLQT;TPVLPHETRLLQTG;P

VLPHETRLLQTGIiVLPHETRLLQTGIHiLPHETRLLQTGIHViPHETRLLQTGIHVRiHET

RLLQTGIHVRViETRLLQTGIHVRVSiTRLLQTGIHVRVSQiRLLQTGIHVRVSQPiLLQT

GIHVRVSQPSiLQTGIHVRVSQPSLiQTGIHVRVSQPSLIiTGIHVRVSQPSLILiGIHVRV

SQPSLILViIHVRVSQPSLILVSiHVRVSQPSLILVSQiVRVSQPSLILVSQYiRVSQPSLIL

VSQYTiVSQPSLILVSQYTPiSQPSLILVSQYTPDiQPSLILVSQYTPDSiPSLILVSQYTP

DSTiSLILVSQYTPDSTPiLILVSQYTPDSTPCiILVSQYTPDSTPCHiLVSQYTPDSTPC

HRiVSQYTPDSTPCHRGiSQYTPDSTPCHRGDiQYTPDSTPCHRGDNiYTPDSTPCH

RGDNQiTPDSTPCHRGDNQLiPDSTPCHRGDNQLQiDSTPCHRGDNQLQViSTPCH

RGDNQLQVQiTPCHRGDNQLQVQHiPCHRGDNQLQVQHTiCHRGDNQLQVQHTY;

HRGDNQLQVQHTYF;RGDNQLQVQHTYFT;GDNQLQVQHTYFTG;DNQLQVQHTYF

TGS;NQLQVQHTYFTGSE;QLQVQHTYFTGSEV;LQVQHTYFTGSEVE;QVQHTYFTG

SEVENiVQHTYFTGSEVENViQHTYFTGSEVENVSiHTYFTGSEVENVSViTYFTGSE

VENVSVNiYFTGSEVENVSVNViFTGSEVENVSVNVHiTGSEVENVSVNVHNiGSEVE

NVSVNVHNPiSEVENVSVNVHNPTiEVENVSVNVHNPTGiVENVSVNVHNPTGRiEN

VSVNVHNPTGRSiNVSVNVHNPTGRSIiVSVNVHNPTGRSICiSVNVHNPTGRSICPiV

NVHNPTGRSICPSiNVHNPTGRSICPSQiVHNPTGRSICPSQEiHNPTGRSICPSQEP;

NPTGRSICPSQEPMiPTGRSICPSQEPMSiTGRSICPSQEPMShGRSICPSQEPMSIY;

RSICPSQEPMSIYViSICPSQEPMSIYVYiICPSQEPMSIYVYAiCPSQEPMSIYVYALiP

SQEPMSIYVYALPiSQEPMSIYVYALPLiQEPMSIYVYALPLKiEPMSIYVYALPLKMiPM

SIYVYALPLKMLiMSIYVYALPLKMLNiSIYVYALPLKMLNIiIYVYALPLKMLNIPiYVYAL

PLKMLN IPSiVYALPLKMLNIPSIiYALPLKMLNIPSINiALPLKMLN IPSINViLPLKMLNIP

SINVHiPLKMLNIPSINVHH iLKMLNIPSINVHHYiKMLNIPSINVHHYPiMLNIPSINVHHY

PSiLNIPSINVHHYPSAiNIPSINVHHYPSAAiIPSINVHHYPSAAEiPSINVHHYPSAAER;

SINVHHYPSAAERKJNVHHYPSAAERKHiNVHHYPSAAERKHRiVHHYPSAAERKHR

HiHHYPSAAERKHRHLiHYPSAAERKHRHLPiYPSAAERKHRHLPViPSAAERKHRHL

PVAiSAAERKHRHLPVADiAAERKHRHLPVADAiAERKHRHLPVADAViERKHRHLPV

ADAVIiRKHRHLPVADAVIHiKHRHLPVADAVIHAiHRHLPVADAVIHASiRHLPVADAVI

HASGiHLPVADAVIHASGKiLPVADAVIHASGKQiPVADAVIHASGKQMiVADAVIHAS

GKQMW ;ADAVIHASGKQMWQ;DAVIHASGKQMWQA;AVIHASGKQMWQAR;VIHAS

GKQMWQARL;IHASGKQMWQARLT;HASGKQMWQARLTV;ASGKQMWQARLTVS;

SGKQMWQARLTVSG ;GKQMWQARLTVSGL;KQMWQARLTVSGLA;QMWQARLTVS GLAW;MWQARLTVSGLAWT;WQARLTVSGLAWTR;QARLTVSG LAWTRQ ;ARLTVS

GLAWTRQQiRLTVSGLAWTRQQNjLTVSGLAWTRQQNQiTVSGLAWTRQQNQWjVS

GLAWTRQQNQWK;SGLAWTRQQNQWKE;GLAWTRQQNQWKEP;LAWTRQQNQW

KEPD;AWTRQQNQWKEPDV;WTRQQNQWKEPDVY;TRQQNQWKEPDVYY;RQQN

QWKEPDVYYT;QQNQWKEPDVYYTS;QNQWKEPDVYYTSA;NQW KEPDVYYTSAF;

QWKEPDVYYTSAFV;WKEPDVYYTSAFVF;KEPDVYYTSAFVFP;EPDVYYTSAFVFP

TiPDVYYTSAFVFPTKjDVYYTSAFVFPTKDjVYYTSAFVFPTKDVjYYTSAFVFPTKDV

AjYTSAFVFPTKDVALjTSAFVFPTKDVALRiSAFVFPTKDVALRHjAFVFPTKDVALRH

VjFVFPTKDVALRHVViVFPTKDVALRHVVCiFPTKDVALRHVVCAjPTKDVALRHVVC

AHiTKDVALRHVVCAHEjKDVALRHVVCAHELjDVALRHVVCAHELViVALRHVVCAH

ELVCiALRHVVCAHELVCSiLRHVVCAHELVCSMjRHVVCAHELVCSMEjHVVCAHEL

VCSMENiVVCAHELVCSMENTjVCAHELVCSMENTRiCAHELVCSMENTRAjAHELV

CSMENTRATiHELVCSMENTRATKjELVCSMENTRATKMjLVCSMENTRATKMQiVC

SMENTRATKMQVjCSMENTRATKMQVIjSMENTRATKMQVIGjMENTRATKMQVIGD;

ENTRATKMQVIGDQjNTRATKMQVIGDQYjTRATKMQVIGDQYVjRATKMQVIGDQYV

KiATKMQVIGDQYVKVjTKMQVIGDQYVKVYjKMQVIGDQYVKVYLjMQVIGDQYVKV

YLEiQVIGDQYVKVYLESjVIGDQYVKVYLESFjlGDQYVKVYLESFCjGDQYVKVYLES

FCEjDQYVKVYLESFCEDiQYVKVYLESFCEDViYVKVYLESFCEDVPjVKVYLESFCE

DVPSiKVYLESFCEDVPSGiVYLESFCEDVPSGKjYLESFCEDVPSGKLjLESFCEDVP

SGKLFjESFCEDVPSGKLFMiSFCEDVPSGKLFMHjFCEDVPSGKLFMHViCEDVPSG

KLFMHVTjEDVPSGKLFMHVTLjDVPSGKLFMHVTLGiVPSGKLFMHVTLGSjPSGKLF

MHVTLGSDiSGKLFMHVTLGSDVjGKLFMHVTLGSDVEjKLFMHVTLGSDVEEjLFMH

VTLGSDVEEDiFMHVTLGSDVEEDLjMHVTLGSDVEEDLTjHVTLGSDVEEDLTMjVTL

GSDVEEDLTMTjTLGSDVEEDLTMTRjLGSDVEEDLTMTRNiGSDVEEDLTMTRNPjS

DVEEDLTMTRNPQjDVEEDLTMTRNPQPjVEEDLTMTRNPQPFjEEDLTMTRNPQPF

MiEDLTMTRNPQPFMRjDLTMTRNPQPFMRPjLTMTRNPQPFMRPHjTMTRNPQPF

MRPHEjMTRNPQPFMRPHERjTRNPQPFMRPHERNjRNPQPFMRPHERNGiNPQPF

MRPHERNGFjPQPFMRPHERNGFTiQPFMRPHERNGFTVjPFMRPHERNGFTVLjFM

RPHERNGFTVLCiMRPHERNGFTVLCPjRPHERNGFTVLCPKjPHERNGFTVLCPKN;

HERNGFTVLCPKNMiERNGFTVLCPKNMIiRNGFTVLCPKNMIIiNGFTVLCPKNMIIK;

GFTVLCPKNMIIKPiFTVLCPKNMIIKPGiTVLCPKNMIIKPGKiVLCPKNMIIKPGKhLCP

KNMIIKPGKISiCPKNMIIKPGKISHiPKNMIIKPGKISHIiKNMIIKPGKISHIMiNMIIKPGKI

SHIMLiMIIKPGKISHIMLDiIIKPGKISHIMLDViIKPGKISHIMLDVAiKPGKISHIMLDVAF;

PGKISHIMLDVAFTiGKISHIMLDVAFTSiKISH IMLDVAFTSHiISHIMLDVAFTSHEiSHI

MLDVAFTSHEHiHIMLDVAFTSHEHFiIMLDVAFTSHEHFGiMLDVAFTSHEHFGLiLDV

AFTSHEHFGLLiDVAFTSHEHFGLLCiVAFTSHEHFGLLCPiAFTSHEHFGLLCPKiFTS

HEHFGLLCPKSiTSHEHFGLLCPKSIiSHEHFGLLCPKSIPiHEHFGLLCPKSIPGiEHFG

LLCPKSIPGLiHFGLLCPKSIPGLSiFGLLCPKSIPGLSIiGLLCPKSIPGLSISiLLCPKSIP

GLSISGiLCPKSIPGLSISGNiCPKSIPGLSISGNLiPKSIPGLSISGNLLiKSIPGLSISGNL

LMiSIPGLSISGNLLMNiIPGLSISGNLLMNGiPGLSISGNLLMNGQiGLSISGNLLMNGQ

QiLSISGNLLMNGQQIiSISGNLLMNGQQIFiISGNLLMNGQQIFLiSGNLLMNGQQIFLE iGNLLMNGQQIFLEViNLLMNGQQIFLEVQiLLMNGQQIFLEVQAiLMNGQQIFLEVQAI;

MNGQQIFLEVQAIRiNGQQIFLEVQAIREiGQQIFLEVQAIRETiQQIFLEVQAIRETViQI

FLEVQAIRETVEiIFLEVQAIRETVELiFLEVQAIRETVELRiLEVQAIRETVELRQiEVQAI

RETVELRQYiVQAIRETVELRQYDiQAIRETVELRQYDPiAIRETVELRQYDPViIRETV

ELRQYDPVA;RETVELRQYDPVAA;ETVELRQYDPVAAL;TVELRQYDPVAALF;VELR

QYDPVAALFFiELRQYDPVAALFFFiLRQYDPVAALFFFDiRQYDPVAALFFFDIiQYDP

VAALFFFDIDiYDPVAALFFFDIDLiDPVAALFFFDIDLLiPVAALFFFDIDLLLiVAALFFFD

IDLLLQiAALFFFDIDLLLQRiALFFFDIDLLLQRGiLFFFDIDLLLQRGPiFFFDIDLLLQRG

PQiFFDIDLLLQRGPQYiFDIDLLLQRGPQYSiDIDLLLQRGPQYSEiIDLLLQRGPQYSE

H;DLLLQRGPQYSEHP;LLLQRGPQYSEHPT;LLQRGPQYSEHPTF;LQRGPQYSEHP

TFTiQRGPQYSEHPTFTSiRGPQYSEHPTFTSQiGPQYSEHPTFTSQYiPQYSEHPTF

TSQYRiQYSEHPTFTSQYRIiYSEHPTFTSQYRIQiSEHPTFTSQYRIQGiEHPTFTSQY

RIQGKiHPTFTSQYRIQGKLiPTFTSQYRIQGKLEiTFTSQYRIQGKLEYiFTSQYRIQGK

LEYRiTSQYRIQGKLEYRHiSQYRIQGKLEYRHTiQYRIQGKLEYRHTWiYRIQGKLEY

RHTWDiRIQG KLEYRHTWDR;IQG KLEYRHTWDRH;QGKLEYRHTWDRHD;GKLEYR

HTWDRHDE;KLEYRHTWDRHDEG;LEYRHTWDRHDEGA;EYRHTWDRHDEGAA;YR

HTWDRHDEGAAQ;RHTWDRHDEGAAQG;HTWDRHDEGAAQGD;TWDRHDEGAAQ GDD;WDRHDEGAAQGDDD;DRHDEGAAQGDDDV;RHDEGAAQGDDDVW;HDEGA

AQG DDDVWT;DEG AAQG DDDVWTS;EG AAQG DDDVWTSG ;G AAQG DDDVWTSGS

;AAQGDDDVWTSGSD;AQGDDDVWTSGSDS;QGDDDVWTSGSDSD;GDDDVWTSG

SDSDE;DDDVWTSGSDSDEE;DDVWTSGSDSDEEL;DVWTSGSDSDEELV;VWTSG

SDSDEELVT ;WTSGSDSDEELVTT;TSGSDSDEELVTTE;SGSDSDEELVTTER;GSDS

DEELVTTERKiSDSDEELVTTERKTjDSDEELVTTERKTPjSDEELVTTERKTPRjDEEL

VTTERKTPRV;EELVTTERKTPRVT;ELVTTERKTPRVTG;LVTTERKTPRVTGG;VTTE

RKTPRVTGGG;TTERKTPRVTGGGA;TERKTPRVTGGGAM;ERKTPRVTGGGAMA;R

KTPRVTGGGAMASjKTPRVTGGGAMASAjTPRVTGGGAMASASjPRVTGGGAMASA

STjRVTGGGAMASASTSjVTGGGAMASASTSAjTGGGAMASASTSAGiGGGAMASA

STSAGRiGGAMASASTSAGRKjGAMASASTSAGRKRjAMASASTSAGRKRKjMASAS

TSAGRKRKSjASASTSAGRKRKSAjSASTSAGRKRKSASjASTSAGRKRKSASSiSTS

AGRKRKSASSAiTSAGRKRKSASSATjSAGRKRKSASSATAjAGRKRKSASSATACjG

RKRKSASSATACTjRKRKSASSATACTAjKRKSASSATACTAGiRKSASSAT ACTAGV;

KSASSAT ACTAGVMiSASSATACTAGVMTjASSATACTAGVMTRjSSATACTAGVMT

RGiSATACTAGVMTRGRiATACTAGVMTRGRLjTACTAGVMTRGRLKjACTAGVMTR

GRLKAiCTAGVMTRGRLKAEjTAGVMTRGRLKAESiAGVMTRGRLKAESTjGVMTRG

RLKAESTViVMTRGRLKAESTVAiMTRGRLKAESTVAPiTRGRLKAESTVAPEjRGRLK

AESTVAPEEiGRLKAESTVAPEEDiRLKAESTVAPEEDTiLKAESTVAPEEDTDiKAES

TVAPEEDTDEiAESTVAPEEDTDEDiESTVAPEEDTDEDSiSTVAPEEDTDEDSDiTVA

PEEDTDEDSDNiVAPEEDTDEDSDNEiAPEEDTDEDSDNEIiPEEDTDEDSDNEIHiEE

DTDEDSDNEIHNiEDTDEDSDNEIHNPiDTDEDSDNEIHNPAiTDEDSDNEIHNPAViD

EDSDNEIHNPAVFiEDSDNEIHNPAVFTiDSDNEIHNPAVFTWiSDNEIHNPAVFTWP;

DNEIHNPAVFTWPPiNEIHNPAVFTWPPWiEIHNPAVFTWPPWQiIHNPAVFTWPPW

QAiHNPAVFTWPPWQAGiNPAVFTWPPWQAGIiPAVFTWPPWQAGILiAVFTWPPW

QAGILAiVFTWPPWQAGILARiFTWPPWQAGILARNiTWPPWQAGILARNLiWPPWQ

AGILARNLViPPWQAGILARNLVPiPWQAGILARNLVPMiWQAGILARNLVPMViQAGIL

ARNLVPMVAiAGILARNLVPMVATiGILARNLVPMVATViILARNLVPMVATVQiLARNL

VPMVATVQG;ARNLVPMVATVQGQ;RNLVPMVATVQGQN;NLVPMVATVQGQNL;LV

PMVATVQGQNLK;VPMVATVQGQNLKY;PMVATVQGQNLKYQ;MVATVQGQNLKYQ

E;VATVQGQNLKYQEF;ATVQGQNLKYQEFF;TVQGQNLKYQEFFW;VQGQNLKYQE

FFWD;QGQNLKYQEFFWDA;GQNLKYQEFFWDAN;QNLKYQEFFWDAND;NLKYQE

FFWDANDIiLKYQEFFWDANDIYiKYQEFFWDANDIYRiYQEFFWDANDIYRIiQEFFW

DANDIYRIFiEFFWDANDIYRIFAiFFWDANDIYRIFAEiFWDANDIYRIFAELiWDANDIY

RIFAELEiDANDIYRIFAELEGiANDIYRIFAELEGViNDIYRIFAELEGVWiDIYRIFAELE

GVWQiIYRIFAELEGVWQPiYRIFAELEGVWQPAiRIFAELEGVWQPAAiIFAELEGVW

QPAAQ;FAELEGVWQPAAQP;AELEGVWQPAAQPK;ELEGVWQPAAQPKR;LEGVW

QPAAQPKRR;EGVWQPAAQPKRRR;G VWQPAAQPKRRRH;VWQPAAQPKRRRHR;

WQPAAQPKRRRHRQ;QPAAQPKRRRHRQD;PAAQPKRRRHRQDA;AAQPKRRRHR

QDALiAQPKRRRHRQDALPiQPKRRRHRQDALPGiPKRRRHRQDALPGPiKRRRHR

QDALPGPCiRRRHRQDALPGPCIiRRHRQDALPGPCIAiRHRQDALPGPCIASiHRQD

ALPGPCIASTiRQDALPG PCIASTPiQDALPG PCIASTPKiDALPG PCIASTPKKiALPGP

CIASTPKKHiLPGPCIASTPKKHRiPGPCIASTPKKHRG;

15 mers:

MESRGRRCPEMISVLiESRGRRCPEMISVLGiSRGRRCPEMISVLGPiRGRRCPEMIS

VLGPliGRRCPEMISVLGPISiRRCPEMISVLGPISGiRCPEMISVLGPISGHiCPEMISVL

GPISGHViPEMISVLGPISGHVLiEMISVLGPISGHVLKiMISVLGPISGHVLKAiISVLGPI

SGHVLKAViSVLGPISGHVLKAVFiVLGPISGHVLKAVFSiLGPISGHVLKAVFSRiGPIS

GHVLKAVFSRGiPISGHVLKAVFSRGDiISGHVLKAVFSRGDTiSGHVLKAVFSRGDTP

;GHVLKAVFSRGDTPV;HVLKAVFSRGDTPVL;VLKAVFSRGDTPVLP;LKAVFSRGDT

PVLPHiKAVFSRGDTPVLPHEiAVFSRGDTPVLPHETiVFSRGDTPVLPHETRiFSRGD

TPVLPHETRLiSRGDTPVLPHETRLLiRGDTPVLPHETRLLQiGDTPVLPHETRLLQTiD

TPVLPHETRLLQTGiTPVLPHETRLLQTGIiPVLPHETRLLQTGIHiVLPHETRLLQTGIH

ViLPHETRLLQTGIHVRiPHETRLLQTGIHVRViHETRLLQTGIHVRVSiETRLLQTGIHV

RVSQiTRLLQTGIHVRVSQPiRLLQTGIHVRVSQPSiLLQTGIHVRVSQPSLiLQTGIHV

RVSQPSLIiQTGIHVRVSQPSLILiTGIHVRVSQPSLILViGIHVRVSQPSLILVSiIHVRVS

QPSLILVSQiHVRVSQPSLILVSQYiVRVSQPSLILVSQYTiRVSQPSLILVSQYTPiVSQ O

PSLILVSQYTPDjSQPSLILVSQYTPDSiQPSLILVSQYTPDSTjPSLILVSQYTPDSTPjS

LILVSQYTPDSTPCiLILVSQYTPDSTPCHjILVSQYTPDSTPCHRjLVSQYTPDSTPCH

RGiVSQYTPDSTPCHRGDjSQYTPDSTPCHRGDNiQYTPDSTPCHRGDNQjYTPDST

PCHRGDNQLjTPDSTPCHRGDNQLQiPDSTPCHRGDNQLQVjDSTPCHRGDNQLQV

QiSTPCHRGDNQLQVQHjTPCHRGDNQLQVQHTjPCHRGDNQLQVQHTYiCHRGDN

QLQVQHTYF;HRGDNQLQVQHTYFT;RGDNQLQVQHTYFTG;GDNQLQVQHTYFTG

S;DNQLQVQHTYFTGSE;NQLQVQHTYFTGSEV;QLQVQHTYFTGSEVE;LQVQHTYF

TGSEVENjQVQHTYFTGSEVENVjVQHTYFTGSEVENVSiQHTYFTGSEVENVSVjHT

YFTGSEVENVSVN ;TYFTGSEVENVSVN V;YFTGSEVENVSVN VH ;FTGSEVENVSVN

VHNjTGSEVENVSVNVHNPiGSEVENVSVNVHNPTiSEVENVSVNVHNPTGiEVENVS

VNVHNPTGRiVENVSVNVHNPTGRSiENVSVNVHNPTGRSIiNVSVNVHNPTGRSIC;

VSVNVHNPTGRSICPiSVNVHNPTGRSICPSiVNVHNPTGRSICPSQiNVHNPTGRSIC

PSQEiVHNPTGRSICPSQEPiHNPTGRSICPSQEPMiNPTGRSICPSQEPMSiPTGRSI

CPSQEPMSIiTGRSICPSQEPMSIYiGRSICPSQEPMSIYViRSICPSQEPMSIYVYiSIC

PSQEPMSIYVYAilCPSQEPMSIYVYALiCPSQEPMSIYVYALPiPSQEPMSIYVYALPL;

SQEPMSIYVYALPLKiQEPMSIYVYALPLKMiEPMSIYVYALPLKMLiPMSIYVYALPLK

MLNiMSIYVYALPLKMLN IiSIYVYALPLKMLNIPiIYVYALPLKMLNIPSiYVYALPLKMLN

IPSIiVYALPLKMLNIPSINiYALPLKMLNIPSINViALPLKMLNIPSINVHiLPLKMLNIPSIN

VHHiPLKMLNIPSINVHHYiLKMLNIPSINVHHYPiKMLNIPSINVHHYPSiMLNIPSINVH

HYPSAiLNIPSINVHHYPSAAiNIPSINVHHYPSAAEiIPSINVHHYPSAAERiPSINVHHY

PSAAERKiSINVHHYPSAAERKHiINVHHYPSAAERKHRiNVHHYPSAAERKHRHiVH

HYPSAAERKHRHLiHHYPSAAERKHRHLPiHYPSAAERKHRHLPViYPSAAERKHRH

LPVAiPSAAERKHRHLPVADiSAAERKHRHLPVADAiAAERKHRHLPVADAViAERKH

RHLPVADAVIiERKHRHLPVADAVIHiRKHRHLPVADAVIHAiKHRHLPVADAVIHASiH

RHLPVADAVIHASGiRHLPVADAVIHASGKiHLPVADAVIHASGKQiLPVADAVIHASGK

QMiPVADAVIHASGKQMWiVADAVIHASGKQMWQiADAVIHASGKQMWQAiDAVIHA

SGKQMWQAR;AVIHASGKQMWQARL;VIHASGKQMWQARLT;IHASGKQMWQARLT

V;HASGKQMWQARLTVS;ASGKQMWQARLTVSG;SGKQMWQARLTVSGL;GKQMW

QARLTVSG LA;KQMWQARLTVSG LAW;QMWQARLTVSGLAWT;MWQARLTVSGLA

WTR;WQARLTVSG LAWTRQ;QARLTVSGLAWTRQQ;ARLTVSG LAWTRQQN;RLTV

SGLAWTRQQNQ;LTVSG LAWTRQQNQW;TVSG LAWTRQQNQWK;VSGLAWTRQQ

NQWKE;SGLAWTRQQNQWKEP;G LAWTRQQNQWKEPD;LAWTRQQNQWKEPDV;

AWTRQQNQWKEPDVY;WTRQQNQWKEPDVYY;TRQQNQWKEPDVYYT;RQQNQ

WKEPDVYYTSiQQNQW KEPDVYYTSA;QNQWKEPDVYYTSAF;NQWKEPDVYYTSA

FViQWKEPDVYYTSAFVFiWKEPDVYYTSAFVFPiKEPDVYYTSAFVFPTiEPDVYYTS

AFVFPTKiPDVYYTSAFVFPTKDiDVYYTSAFVFPTKDViVYYTSAFVFPTKDVAiYYTS

AFVFPTKDVALiYTSAFVFPTKDVALRiTSAFVFPTKDVALRHiSAFVFPTKDVALRHV;

AFVFPTKDVALRHVViFVFPTKDVALRHVVCiVFPTKDVALRHVVCAiFPTKDVALRHV

VCAHiPTKDVALRHVVCAHEiTKDVALRHVVCAHELiKDVALRHVVCAHELViDVALRH

VVCAHELVCiVALRHVVCAHELVCSiALRHVVCAHELVCSMiLRHVVCAHELVCSME;

RHVVCAHELVCSMENiHVVCAHELVCSMENTiVVCAHELVCSMENTRiVCAHELVCS

MENTRAiCAHELVCSMENTRATiAHELVCSMENTRATKiHELVCSMENTRATKMiELV

CSMENTRATKMQiLVCSMENTRATKMQViVCSMENTRATKMQVIiCSMENTRATKM

QVIGiSMENTRATKMQVIGDiMENTRATKMQVIGDQiENTRATKMQVIGDQYiNTRAT

KMQVIGDQYViTRATKMQVIGDQYVKiRATKMQVIGDQYVKViATKMQVIGDQYVKVY

;TKMQVIGDQYVKVYL;KMQVIGDQYVKVYLE;MQVIGDQYV KVYLESiQVIGDQYVKV

YLESFiVIGDQYVKVYLESFCilGDQYVKVYLESFCEiGDQYVKVYLESFCEDiDQYVK

VYLESFCEDViQYVKVYLESFCEDVPiYVKVYLESFCEDVPSiVKVYLESFCEDVPSG;

KVYLESFCEDVPSGKiVYLESFCEDVPSGKLiYLESFCEDVPSGKLFiLESFCEDVPSG

KLFMiESFCEDVPSGKLFMHiSFCEDVPSGKLFMHViFCEDVPSGKLFMHVTiCEDVP

SGKLFMHVTLiEDVPSGKLFMHVTLGiDVPSGKLFMHVTLGSiVPSGKLFMHVTLGSD

;PSGKLFMHVTLGSDV;SGKLFMHVTLGSDVE;GKLFMHVTLGSDVEE;KLFMHVTLG

SDVEEDiLFMHVTLGSDVEEDLiFMHVTLGSDVEEDLTiMHVTLGSDVEEDLTMiHVTL

GSDVEEDLTMTiVTLGSDVEEDLTMTRiTLGSDVEEDLTMTRNiLGSDVEEDLTMTRN

PiGSDVEEDLTMTRNPQiSDVEEDLTMTRNPQPiDVEEDLTMTRNPQPFiVEEDLTMT

RNPQPFMiEEDLTMTRNPQPFMRiEDLTMTRNPQPFMRPiDLTMTRNPQPFMRPHiL

TMTRNPQPFMRPHEiTMTRNPQPFMRPHERiMTRNPQPFMRPHERNiTRNPQPFM

RPHERNG;RNPQPFMRPHERNGF;NPQPFMRPHERNGFT;PQPFMRPHERNGFTV; QPFMRPHERNGFTVLjPFMRPHERNGFTVLCjFMRPHERNGFTVLCPjMRPHERNG

FTVLCPKiRPHERNGFTVLCPKNiPHERNGFTVLCPKNMiHERNGFTVLCPKNMIiER

NGFTVLCPKNMIIiRNGFTVLCPKNMIIKiNGFTVLCPKNMIIKPiGFTVLCPKNMIIKPG;

FTVLCPKNMIIKPGKiTVLCPKNMIIKPGKIiVLCPKNMIIKPGKISiLCPKNMIIKPGKISH;

CPKNMIIKPGKISHIiPKNMIIKPGKISHIMiKNMIIKPGKISHIMLiNMIIKPGKISHIMLDiMI

IKPGKISHIMLDViIIKPGKISHIMLDVAiIKPGKISHIMLDVAFiKPGKISHIMLDVAFTiPG

KISHIMLDVAFTSiGKISHIMLDVAFTSHiKISHIMLDVAFTSHEiISHIMLDVAFTSHEHiS

HIMLDVAFTSHEHFiHIMLDVAFTSHEHFGiIMLDVAFTSHEHFGLiMLDVAFTSHEHF

GLLiLDVAFTSHEHFGLLCiDVAFTSHEHFGLLCPiVAFTSHEHFGLLCPKiAFTSHEHF

GLLCPKSiFTSHEHFGLLCPKSIiTSHEHFGLLCPKSIPiSHEHFGLLCPKSIPGiHEHFG

LLCPKSIPGLiEHFGLLCPKSIPGLSiHFGLLCPKSIPGLSIiFGLLCPKSIPGLSISiGLLC

PKSIPGLSISGiLLCPKSIPGLSISGNiLCPKSIPGLSISGNLiCPKSIPGLSISGNLLiPKSI

PGLSISGNLLMiKSIPGLSISGNLLMNiSIPGLSISGNLLMNGiIPGLSISGNLLMNGQiPG

LSISGNLLMNGQQiGLSISGNLLMNGQQIiLSISGNLLMNGQQIFiSISGNLLMNGQQIF

LiISGNLLMNGQQIFLEiSGNLLMNGQQIFLEViGNLLMNGQQIFLEVQiNLLMNGQQIF

LEVQAiLLMNGQQIFLEVQAIiLMNGQQIFLEVQAIRiMNGQQIFLEVQAIREiNGQQIFL

EVQAIRETiGQQIFLEVQAIRETViQQIFLEVQAIRETVEiQIFLEVQAIRETVELiIFLEVQ

AIRETVELRiFLEVQAIRETVELRQiLEVQAIRETVELRQYiEVQAIRETVELRQYDiVQA

IRETVELRQYDPiQAIRETVELRQYDPViAIRETVELRQYDPVAiIRETVELRQYDPVAA;

RETVELRQYDPVAALiETVELRQYDPVAALFiTVELRQYDPVAALFFiVELRQYDPVAA

LFFFiELRQYDPVAALFFFDiLRQYDPVAALFFFDIiRQYDPVAALFFFDIDiQYDPVAAL

FFFDIDLiYDPVAALFFFDIDLLiDPVAALFFFDIDLLLiPVAALFFFDIDLLLQiVAALFFFD

IDLLLQRiAALFFFDIDLLLQRGiALFFFDIDLLLQRGPiLFFFDIDLLLQRGPQiFFFDIDL

LLQRGPQYiFFDIDLLLQRGPQYSiFDIDLLLQRGPQYSEiDIDLLLQRGPQYSEHiIDLL

LQRGPQYSEHP;DLLLQRGPQYSEHPT;LLLQRGPQYSEHPTF;LLQRGPQYSEHPTF

T;LQRGPQYSEHPTFTS;QRGPQYSEHPTFTSQ;RGPQYSEHPTFTSQY;G PQYSEH

PTFTSQYRiPQYSEHPTFTSQYRIiQYSEHPTFTSQYRIQiYSEHPTFTSQYRIQGiSEH

PTFTSQYRIQGKiEHPTFTSQYRIQGKLiHPTFTSQYRIQGKLEiPTFTSQYRIQGKLEY

;TFTSQYRIQGKLEYR;FTSQYRIQGKLEYRH;TSQYRIQG KLEYRHTiSQYRIQGKLEY

RHTW ;QYRIQGKLEYRHTWD;YRIQGKLEYRHTWDR;RIQG KLEYRHTWDRHiIQG KL

EYRHTWDRHD;QG KLEYRHTWDRHDE;GKLEYRHTWDRHDEG;KLEYRHTWDRHD

EGA;LEYRHTWDRHDEGAA;EYRHTWDRHDEGAAQ;YRHTWDRHDEGAAQG;RHT

WDRHDEGAAQGD;HTWDRHDEGAAQGDD;TWDRHDEGAAQGDDD;WDRHDEGA

AQGDDDV;DRHDEGAAQGDDDVW;RHDEGAAQGDDDVWT;HDEGAAQGDDDVWT

S;DEGAAQGDDDVWTSG;EGAAQGDDDVWTSGS;GAAQGDDDVWTSGSD;AAQGD

DDVWTSGSDS;AQGDDDVWTSGSDSD;QGDDDVWTSGSDSDE;GDDDVWTSGSD

SDEE;DDDVWTSGSDSDEEL;DDVWTSGSDSDEELV;DVWTSGSDSDEELVT;VWTS

GSDSDEELVTT ;WTSGSDSDEELVTTE;TSGSDSDEELVTTER;SGSDSDEELVTTER

K;GSDSDEELVTTERKT;SDSDEELVTTERKTP;DSDEELVTTERKTPR;SDEELVTTE

RKTPRV;DEELVTTERKTPRVT;EELVTTERKTPRVTG;ELVTTERKTPRVTGG;LVTTE

RKTPRVTGGG;VTTERKTPRVTGGGA;TTERKTPRVTGGGAM;TERKTPRVTGGGA

MAiERKTPRVTGGGAMASiRKTPRVTGGGAMASAiKTPRVTGGGAMASASiTPRVTG

GGAMASAST;PRVTGGGAMASASTS;RVTGGGAMASASTSA;VTGGGAMASASTSA

GiTGGGAMASASTSAGRiGGGAMASASTSAGRKiGGAMASASTSAGRKRiGAMASA

STSAGRKRK;AMASASTSAGRKRKS;MASASTSAGRKRKSA;ASASTSAGRKRKSAS;

SASTSAGRKRKSASSiASTSAGRKRKSASSAiSTSAGRKRKSASSATiTSAGRKRKSA

SSATAiSAGRKRKSASSATACiAGRKRKSASSATACTiGRKRKSASSATACTAiRKRK

SASSATACTAGiKRKSASSATACTAGViRKSASSATACTAGVMiKSASSATACTAGV

MTiSASSATACTAGVMTRiASSATACTAGVMTRGiSSATACTAGVMTRGRiSATACTA

GVMTRGRLiATACTAGVMTRGRLKiTACTAGVMTRGRLKAiACTAGVMTRGRLKAE;

CTAGVMTRGRLKAESiTAGVMTRGRLKAESTiAGVMTRGRLKAESTViGVMTRGRLK

AESTVAiVMTRGRLKAESTVAPiMTRGRLKAESTVAPEiTRGRLKAESTVAPEEiRGR

LKAESTVAPEEDiGRLKAESTVAPEEDTiRLKAESTVAPEEDTDiLKAESTVAPEEDTD

EiKAESTVAPEEDTDEDiAESTVAPEEDTDEDSiESTVAPEEDTDEDSDiSTVAPEEDT

DEDSDNiTVAPEEDTDEDSDNEiVAPEEDTDEDSDNEIiAPEEDTDEDSDNEIHiPEED

TDEDSDNEIHNiEEDTDEDSDNEIHNPiEDTDEDSDNEIHNPAiDTDEDSDNEIHNPAV

;TDEDSDNEIHNPAVF;DEDSDNEIHNPAVFT;EDSDNEIHNPAVFTW;DSDNEIHNPAV

FTWPiSDNEIHNPAVFTWPPiDNEIHNPAVFTWPPWiNEIHNPAVFTWPPWQjEIHNP o

AVFTWPPWQAiIHNPAVFTWPPWQAGiHNPAVFTWPPWQAGIiNPAVFTWPPWQA

GILiPAVFTWPPWQAGILAjAVFTWPPWQAGILARjVFTWPPWQAGILARN jFTWPPW

QAGILARNLjTWPPWQAGILARNLVjWPPWQAGILARNLVPjPPWQAGILARNLVPM;

PWQAG I LARN LVPM V;WQAG I LARN LVPM VA;Q AG I LARN LVPM VAT ;AG I LARN LVPM

VATVjGILARNLVPMVATVQjlLARNLVPMVATVQGjLARNLVPMVATVQGQiARNLVP

MVATVQGQN;RNLVPMVATVQGQNL;NLVPMVATVQGQNLK;LVPMVATVQGQNLK

Y;VPMVATVQGQNLKYQ;PMVATVQGQNLKYQE;MVATVQGQNLKYQEF;VATVQG

QNLKYQEFF;ATVQGQNLKYQEFFW;TVQGQNLKYQEFFWD;VQGQNLKYQEFFWD

A;QGQNLKYQEFFWDAN;GQNLKYQEFFWDAND;QNLKYQEFFWDANDI;NLKYQEF

FWDANDIYjLKYQEFFWDANDIYRjKYQEFFWDANDIYRIjYQEFFWDANDIYRIFiQEF

FWDANDIYRIFAjEFFWDANDIYRIFAEjFFWDANDIYRIFAELjFWDANDIYRIFAELE;

WDANDIYRIFAELEGjDANDIYRIFAELEGVjANDIYRIFAELEGVWjNDIYRIFAELEGV

WQiDIYRIFAELEGVWQPjlYRIFAELEGVWQPAjYRIFAELEGVWQPAAjRIFAELEGV

WQPAAQ;IFAELEGVWQPAAQP;FAELEGVWQPAAQPK;AELEGVWQPAAQPKR;EL

EGVWQPAAQPKRR;LEGVWQPAAQPKRRR;EGVWQPAAQPKRRRH;GVWQPAAQ

PKRRRHR;VWQPAAQPKRRRHRQ;WQPAAQPKRRRHRQD;QPAAQPKRRRHRQD

A;PAAQPKRRRHRQDAL;AAQPKRRRHRQDALP;AQPKRRRHRQDALPG;QPKRRR

HRQDALPGPiPKRRRHRQDALPGPCiKRRRHRQDALPGPCIiRRRHRQDALPGPCIA;

RRHRQDALPG PCIASiRHRQDALPGPCIASTjHRQDALPGPCIASTPiRQDALPG PCIA

STPK;QDALPG PCIASTPKKiDALPG PCIASTPKKHiALPGPCIASTPKKHRiLPGPCIAS

TPKKHRG;

16 mers:

MESRGRRCPEMISVLGiESRGRRCPEMISVLGPiSRGRRCPEMISVLGPIiRGRRCPE

MISVLGPISiGRRCPEMISVLGPISGiRRCPEMISVLGPISGHiRCPEMISVLGPISGHV;

CPEMISVLGPISGHVLiPEMISVLGPISGHVLKiEMISVLGPISGHVLKAiMISVLGPISGH

VLKAViISVLGPISGHVLKAVFiSVLGPISGHVLKAVFSiVLGPISGHVLKAVFSRiLGPIS

GHVLKAVFSRGiGPISGHVLKAVFSRGDiPISGHVLKAVFSRGDTiISGHVLKAVFSRG

DTPiSGHVLKAVFSRGDTPViGHVLKAVFSRGDTPVLiHVLKAVFSRGDTPVLPiVLKA

VFSRGDTPVLPH iLKAVFSRGDTPVLPHEiKAVFSRGDTPVLPHETiAVFSRGDTPVL

PHETR;VFSRGDTPVLPHETRL;FSRGDTPVLPHETRLL;SRGDTPVLPHETRLLQ;RG

DTPVLPHETRLLQTiGDTPVLPHETRLLQTGiDTPVLPHETRLLQTGIiTPVLPHETRLL

QTGIHiPVLPHETRLLQTGIHViVLPHETRLLQTGIHVRiLPHETRLLQTGIHVRViPHET

RLLQTG I H VRVS ;H ETRLLQTG I H VRVSQ ;ETRLLQTG IH VRVSQP;TRLLQTG I HVRVS

QPSiRLLQTGIHVRVSQPSLiLLQTGIHVRVSQPSLIiLQTGIHVRVSQPSLILiQTGIHVR

VSQPSLILViTGIHVRVSQPSLILVSiGIHVRVSQPSLILVSQiIHVRVSQPSLILVSQYiHV

RVSQPSLILVSQYTiVRVSQPSLILVSQYTPiRVSQPSLILVSQYTPDiVSQPSLILVSQY

TPDSiSQPSLILVSQYTPDSTiQPSLILVSQYTPDSTPiPSLILVSQYTPDSTPCiSLILVS

QYTPDSTPCHiLILVSQYTPDSTPCHRiILVSQYTPDSTPCHRGiLVSQYTPDSTPCHR

GDiVSQYTPDSTPCHRGDNiSQYTPDSTPCHRGDNQiQYTPDSTPCHRGDNQLiYTP

DSTPCHRGDNQLQiTPDSTPCHRGDNQLQViPDSTPCHRGDNQLQVQiDSTPCHRG

DNQLQVQHiSTPCHRGDNQLQVQHTiTPCHRGDNQLQVQHTYiPCHRGDNQLQVQ

HTYFiCHRGDNQLQVQHTYFTiHRGDNQLQVQHTYFTGiRGDNQLQVQHTYFTGSiG

DNQLQVQHTYFTGSE;DNQLQVQHTYFTGSEV;NQLQVQHTYFTGSEVE;QLQVQHT

YFTGSEVENiLQVQHTYFTGSEVENViQVQHTYFTGSEVENVSiVQHTYFTGSEVEN

VSViQHTYFTGSEVENVSVNiHTYFTGSEVENVSVNViTYFTGSEVENVSVNVHiYFT

GSEVENVSVNVHNiFTGSEVENVSVNVHNPiTGSEVENVSVNVHNPTiGSEVENVSV

NVHNPTGiSEVENVSVNVHNPTGRiEVENVSVNVHNPTGRSiVENVSVNVHNPTGRS liENVSVNVHNPTGRSICiNVSVNVHNPTGRSICPiVSVNVHNPTGRSICPSiSVNVHN

PTGRSICPSQiVNVHNPTGRSICPSQEiNVHNPTGRSICPSQEPiVHNPTGRSICPSQ

EPMiHNPTGRSICPSQEPMSiNPTGRSICPSQEPMSIiPTGRSICPSQEPMSIYiTGRSI

CPSQEPMSIYViGRSICPSQEPMSIYVYiRSICPSQEPMSIYVYAiSICPSQEPMSIYVY

ALilCPSQEPMSIYVYALPiCPSQEPMSIYVYALPLiPSQEPMSIYVYALPLKiSQEPMSI

YVYALPLKMiQEPMSIYVYALPLKMLiEPMSIYVYALPLKMLNiPMSIYVYALPLKMLNI;

MSIYVYALPLKMLNIPiSIYVYALPLKMLNIPSiIYVYALPLKMLNIPShYVYALPLKMLNIP

SINiVYALPLKMLNIPSINViYALPLKMLNIPSINVH iALPLKMLNIPSINVHHiLPLKMLNIP

SINVHHYiPLKMLNIPSINVHHYPiLKMLNIPSINVHHYPSiKMLNIPSINVHHYPSAiMLN

IPSINVHHYPSAAiLNIPSINVHHYPSAAEjNIPSINVHHYPSAAERilPSINVHHYPSAAE RKjPSINVHHYPSAAERKH iSINVHHYPSAAERKHRjINVHHYPSAAERKHRHjNVHHY

PSAAERKHRHLjVHHYPSAAERKHRHLPjHHYPSAAERKHRHLPVjHYPSAAERKHR

HLPVAjYPSAAERKHRHLPVADjPSAAERKHRHLPVADAiSAAERKHRHLPVADAVjA

AERKHRHLPVADAVIjAERKHRHLPVADAVIHjERKHRHLPVADAVIHAjRKHRHLPVA

DAVIHASiKHRHLPVADAVIHASGjHRHLPVADAVIHASGKjRHLPVADAVIHASGKQjH

LPVADAVIHASGKQMjLPVADAVIHASGKQMWjPVADAVIHASGKQMWQjVADAVIHA

SGKQMWQA;ADAVIHASGKQMWQAR;DAVIHASGKQMWQARL;AVIHASGKQMWQ

ARLTiVIHASGKQMWQARLTVjIHASGKQMWQARLTVSjHASGKQMWQARLTVSGjA

SGKQMWQARLTVSGLjSGKQMWQARLTVSGLAjGKQMWQARLTVSGLAWjKQMW

QARLTVSG LAWT;QMWQARLTVSGLAWTR;MWQARLTVSGLAWTRQ;WQARLTVS

GLAWTRQQ ;QARLTVSGLAWTRQQN;ARLTVSGLAWTRQQNQ;RLTVSGLAWTRQ

QNQW;LTVSGLAWTRQQNQWK;TVSGLAWTRQQNQWKE;VSGLAWTRQQNQWKE

P;SGLAWTRQQNQWKEPD;GLAWTRQQNQWKEPDV;LAWTRQQNQWKEPDVY;A

WTRQQNQWKEPDVYYjWTRQQNQWKEPDVYYTjTRQQNQWKEPDVYYTSjRQQN

QWKEPDVYYTSAjQQNQWKEPDVYYTSAFjQNQWKEPDVYYTSAFVjNQW KEPDV

YYTSAFVFjQWKEPDVYYTSAFVFPjWKEPDVYYTSAFVFPTjKEPDVYYTSAFVFPT

KjEPDVYYTSAFVFPTKDiPDVYYTSAFVFPTKDVjDVYYTSAFVFPTKDVAjVYYTSAF

VFPTKDVALjYYTSAFVFPTKDVALRjYTSAFVFPTKDVALRHjTSAFVFPTKDVALRHV iSAFVFPTKDVALRHVVjAFVFPTKDVALRHVVCiFVFPTKDVALRHVVCAjVFPTKDV

ALRHVVCAH iFPTKDVALRHVVCAHEjPTKDVALRHVVCAHELjTKDVALRHVVCAHE

LVjKDVALRHVVCAHELVCiDVALRHVVCAHELVCSiVALRHVVCAHELVCSMjALRHV

VCAHELVCSMEiLRHVVCAHELVCSMENjRHVVCAHELVCSMENTjHVVCAHELVCS

MENTRiVVCAHELVCSMENTRAjVCAHELVCSMENTRATiCAHELVCSMENTRATKjA

HELVCSMENTRATKMjHELVCSMENTRATKMQiELVCSMENTRATKMQVjLVCSME

NTRATKMQVIjVCSMENTRATKMQVIGjCSMENTRATKMQVIGDjSMENTRATKMQVI

GDQiMENTRATKMQVIGDQYjENTRATKMQVIGDQYVjNTRATKMQVIGDQYVKjTRA

TKMQVIGDQYVKVjRATKMQVIGDQYVKVYjATKMQVIGDQYVKVYLjTKMQVIGDQY

VKVYLEjKMQVIGDQYVKVYLESjMQVIGDQYVKVYLESFiQVIGDQYVKVYLESFCjVI

GDQYVKVYLESFCEjlGDQYVKVYLESFCEDiGDQYVKVYLESFCEDVjDQYVKVYLE

SFCEDVPjQYVKVYLESFCEDVPSjYVKVYLESFCEDVPSGiVKVYLESFCEDVPSGK;

KVYLESFCEDVPSGKLiVYLESFCEDVPSGKLFjYLESFCEDVPSGKLFMjLESFCEDV

PSGKLFMHjESFCEDVPSGKLFMHVjSFCEDVPSGKLFMHVTjFCEDVPSGKLFMHV

TLiCEDVPSGKLFMHVTLGiEDVPSGKLFMHVTLGSjDVPSGKLFMHVTLGSDjVPSG

KLFMHVTLGSDVjPSGKLFMHVTLGSDVEjSGKLFMHVTLGSDVEEjGKLFMHVTLGS

DVEEDiKLFMHVTLGSDVEEDLjLFMHVTLGSDVEEDLTjFMHVTLGSDVEEDLTMjM

HVTLGSDVEEDLTMTjHVTLGSDVEEDLTMTRjVTLGSDVEEDLTMTRNjTLGSDVEE

DLTMTRNPjLGSDVEEDLTMTRNPQjGSDVEEDLTMTRNPQPjSDVEEDLTMTRNPQ

PFjDVEEDLTMTRNPQPFMiVEEDLTMTRNPQPFMRjEEDLTMTRNPQPFMRPjEDLT

MTRNPQPFMRPHjDLTMTRNPQPFMRPHEjLTMTRNPQPFMRPHERjTMTRNPQPF

MRPHERNjMTRNPQPFMRPHERNGjTRNPQPFMRPHERNGFjRNPQPFMRPHERN

GFTjNPQPFMRPHERNGFTVjPQPFMRPHERNGFTVLiQPFMRPHERNGFTVLCjPF

MRPHERNGFTVLCPiFMRPHERNGFTVLCPKjMRPHERNGFTVLCPKNjRPHERNGF

TVLCPKNMiPHERNGFTVLCPKNMIjHERNGFTVLCPKNMIIjERNGFTVLCPKNMIIK;

RNGFTVLCPKNMIIKPiNGFTVLCPKNMIIKPGiGFTVLCPKNMIIKPGKjFTVLCPKNMII

KPGKIjTVLCPKNMIIKPGKISiVLCPKNMIIKPGKISHjLCPKNMIIKPGKISHliCPKNMIIK

PGKISHIMjPKNMIIKPGKISHIMLiKNMIIKPGKISHIMLDjNMIIKPGKISHIMLDViMIIKP

GKISHIMLDVAillKPGKISHIMLDVAFjlKPGKISHIMLDVAFTjKPGKISHIMLDVAFTSiP

GKISHIMLDVAFTSHjGKISHIMLDVAFTSHEjKISHIMLDVAFTSHEHjISHIMLDVAFTS

HEHFjSHIMLDVAFTSHEHFGiHIMLDVAFTSHEHFGLjIMLDVAFTSHEHFGLLjMLDV

AFTSHEHFGLLCiLDVAFTSHEHFGLLCPjDVAFTSHEHFGLLCPKjVAFTSHEHFGLL

CPKSiAFTSHEHFGLLCPKSIjFTSHEHFGLLCPKSIPjTSHEHFGLLCPKSIPGiSHEHF

GLLCPKSIPGLjHEHFGLLCPKSIPGLSiEHFGLLCPKSIPGLSIjHFGLLCPKSIPGLSIS;

FGLLCPKSIPGLSISGiGLLCPKSIPGLSISGNjLLCPKSIPGLSISGNLjLCPKSIPGLSIS

GNLLiCPKSIPGLSISGNLLMjPKSIPGLSISGNLLMNjKSIPGLSISGNLLMNGiSIPGLSI

SGNLLMNGQjlPGLSISGNLLMNGQQjPGLSISGNLLMNGQQIjGLSISGNLLMNGQQI

FjLSISGNLLMNGQQIFLiSISGNLLMNGQQIFLEjlSGNLLMNGQQIFLEVjSGNLLMNG

QQIFLEVQiGNLLMNGQQIFLEVQAjNLLMNGQQIFLEVQAIjLLMNGQQIFLEVQAIRjL

MNGQQIFLEVQAIREiMNGQQIFLEVQAIRETiNGQQIFLEVQAIRETViGQQIFLEVQAI o

RETVE iQQIFLEVQAIRETVELjQIFLEVQAIRETVELRjIFLEVQAIRETVELRQjFLEVQA

IRETVELRQYjLEVQAIRETVELRQYDjEVQAIRETVELRQYDPjVQAIRETVELRQYDP

ViQAIRETVELRQYDPVAjAIRETVELRQYDPVAAjIRETVELRQYDPVAALjRETVELR

QYDPVAALFjETVELRQYDPVAALFFjTVELRQYDPVAALFFFjVELRQYDPVAALFFF

DjELRQYDPVAALFFFDIjLRQYDPVAALFFFDIDjRQYDPVAALFFFDIDLiQYDPVAAL

FFFDIDLLjYDPVAALFFFDIDLLLjDPVAALFFFDIDLLLQiPVAALFFFDIDLLLQRjVAAL

FFFDIDLLLQRGjAALFFFDIDLLLQRGPjALFFFDIDLLLQRGPQjLFFFDIDLLLQRGPQ

YiFFFDIDLLLQRGPQYSjFFDIDLLLQRGPQYSEjFDIDLLLQRGPQYSEHjDIDLLLQR

GPQYSEHPjIDLLLQRGPQYSEHPTjDLLLQRGPQYSEHPTFjLLLQRGPQ YSEHPTF

TjLLQRGPQYSEHPTFTSjLQRGPQYSEHPTFTSQiQRGPQYSEHPTFTSQYjRGPQY

SEHPTFTSQYRjGPQYSEHPTFTSQYRIjPQYSEHPTFTSQYRIQjQYSEHPTFTSQY

RIQGiYSEHPTFTSQYRIQGKjSEHPTFTSQYRIQGKLjEHPTFTSQYRIQGKLEjHPTF

TSQYRIQGKLEYjPTFTSQYRIQGKLEYRjTFTSQYRIQGKLEYRHjFTSQYRIQGKLEY

RHTiTSQYRIQGKLEYRHTWjSQYRIQGKLEYRHTWDjQYRIQGKLEYRHTWDRjYRI

QGKLEYRHTWDRH;RIQGKLEYRHTWDRHD;IQGKLEYRHTWDRHDE;QGKLEYRH

TWDRHDEG;GKLEYRHTWDRHDEGA;KLEYRHTWDRHDEGAA;LEYRHTWDRHDE

GAAQ;EYRHTWDRHDEGAAQG;YRHTWDRHDEGAAQGD;RHTWDRHDEGAAQGD

D;HTWDRHDEGAAQGDDD;TWDRHDEGAAQGDDDV;WDRHDEGAAQGDDDVW;D

RHDEGAAQGDDDVWT;RHDEGAAQGDDDVWTS;HDEGAAQGDDDVWTSG;DEGA

AQGDDDVWTSGSjEGAAQGDDDVWTSGSDjGAAQGDDDVWTSGSDSjAAQGDDD

VWTSGSDSDjAQGDDDVWTSGSDSDEjQGDDDVWTSGSDSDEEjGDDDVWTSGS

DSDEELiDDDVWTSGSDSDEELVjDDVWTSGSDSDEELVTjDVWTSGSDSDEELVTT

;VWTSGSDSDEELVTTE;WTSGSDSDEELVTTER;TSGSDSDEELVTTERK;SGSDSD

EELVTTERKT;GSDSDEELVTTERKTP;SDSDEELVTTERKTPR;DSDEELVTTERKTP

RVjSDEELVTTERKTPRVTjDEELVTTERKTPRVTGiEELVTTERKTPRVTGGjELVTTE

RKTPRVTGGGjLVTTERKTPRVTGGGAjVTTERKTPRVTGGGAMjTTERKTPRVTGG

GAMAjTERKTPRVTGGGAMASjERKTPRVTGGGAMASAjRKTPRVTGGGAMASAS;

KTPRVTGGGAMASASTjTPRVTGGGAMASASTSjPRVTGGGAMASASTSAjRVTGG

GAMASASTSAGjVTGGGAMASASTSAGRjTGGGAMASASTSAGRKjGGGAMASAST

SAGRKRjGGAMASASTSAGRKRKjGAMASASTSAGRKRKSjAMASASTSAGRKRKS

AjMASASTSAGRKRKSASjASASTSAGRKRKSASSiSASTSAGRKRKSASSAjASTSA

GRKRKSASSATjSTSAGRKRKSASSATAjTSAGRKRKSASSATACjSAGRKRKSASS

ATACTjAGRKRKSASSATACTAiGRKRKSASSATACTAGiRKRKSASSATACTAGVjK

RKSASSAT ACTAGVMjRKSASSATACTAGVMTjKSASSATACTAGVMTRiSASSATA

CTAGVMTRGjASSATACTAGVMTRGRiSSATACTAGVMTRGRLjSATACTAGVMTR

GRLKiATACTAGVMTRGRLKAjTACTAGVMTRGRLKAEjACTAGVMTRGRLKAESiCT

AGVMTRGRLKAESTjTAGVMTRGRLKAESTVjAGVMTRGRLKAESTVAjG VMTRGRL

KAESTVAPjVMTRGRLKAESTVAPEjMTRGRLKAESTVAPEEjTRGRLKAESTVAPEE

DjRGRLKAESTVAPEEDTiGRLKAESTVAPEEDTDjRLKAESTVAPEEDTDEjLKAEST

VAPEEDTDEDjKAESTVAPEEDTDEDSjAESTVAPEEDTDEDSDjESTVAPEEDTDED

SDNiSTVAPEEDTDEDSDNEjTVAPEEDTDEDSDNEIjVAPEEDTDEDSDNEIHjAPEE

DTDEDSDNEIHNjPEEDTDEDSDNEIHNPjEEDTDEDSDNEIHNPAjEDTDEDSDNEIH

NPAViDTDEDSDNEIHNPAVFjTDEDSDNEIHNPAVFTjDEDSDNEIHNPAVFTWjEDS

DNEIHNPAVFTWPjDSDNEIHNPAVFTWPPjSDNEIHNPAVFTWPPWjDNEIHNPAVF

TWPPWQjNEIHNPAVFTWPPWQAjEIHNPAVFTWPPWQAGilHNPAVFTWPPWQAG

IjHNPAVFTWPPWQAGILiNPAVFTWPPWQAGILAiPAVFTWPPWQAGILARjAVFTW

PPWQAGILARNjVFTWPPWQAGILARNLjFTWPPWQAGILARNLVjTWPPWQAGILA

RNLVPjWPPWQAGILARNLVPMjPPWQAGILARNLVPMVjPWQAGILARNLVPMVA;

WQAG I LARN LVPM VAT;QAG I LARN LVPM VATV; AG I LARN LVPM VATVQ ;G I LARN LV

PMVATVQGjlLARNLVPMVATVQGQjLARNLVPMVATVQGQNjARNLVPMVATVQGQ

NL;RNLVPMVATVQGQNLK;NLVPMVATVQGQNLKY;LVPMVATVQGQNLKYQ;VPM

VATVQGQNLKYQE;PMVATVQGQNLKYQEF;MVATVQGQNLKYQEFF;VATVQGQN

LKYQEFFW ;ATVQGQNLKYQEFFWD;TVQGQNLKYQEFFWDA;VQGQNLKYQEFFW

DAN;QGQNLKYQEFFWDAND;GQNLKYQEFFWDANDI;QNLKYQEFFWDANDIY;NL

KYQEFFWDANDIYRjLKYQEFFWDANDIYRIjKYQEFFWDANDIYRIFjYQEFFWDAND lYRIFAiQEFFWDANDIYRIFAEjEFFWDANDIYRIFAELjFFWDANDIYRIFAELEjFWDA

NDIYRIFAELEGjWDANDIYRIFAELEGVjDANDIYRIFAELEGVWjANDIYRIFAELEGV

WQiNDIYRIFAELEGVWQPiDIYRIFAELEGVWQPAilYRIFAELEGVWQPAAjYRIFAEL o

EGVWQPAAQ;RIFAELEGVWQPAAQP;IFAELEGVWQPAAQPK;FAELEGVWQPAAQ PKR;AELEGVWQPAAQPKRR;ELEGVWQPAAQPKRRR;LEGVWQPAAQPKRRRH;E GVWQPAAQPKRRRHR;GVWQPAAQPKRRRHRQ;VWQPAAQPKRRRHRQD;WQPA AQPKRRRHRQDA;QPAAQPKRRRHRQDAL;PAAQPKRRRHRQDALP;AAQPKRRRH RQDALPG ;AQPKRRRHRQDALPGP;QPKRRRHRQDALPGPC;PKRRRHRQDALPGP CliKRRRHRQDALPGPCIAiRRRHRQDALPGPCIASiRRHRQDALPGPCIASTjRHRQD ALPGPCIASTPiHRQDALPGPCIASTPKiRQDALPGPCIASTPKKiQDALPGPCIASTPK KHjDALPG PCIASTPKKH RJALPG PCIASTPKKHRG

<YP_081562.1 regulatory protein IE1 ;Human herpesvirus 5> 4402-

MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFP 8362

ELAEESLKTFEQVTEDCNENPEKDVLTELVKQIKVRVDMVRHRIKEHMLKKYTQTEE

KFTGAFNMMGGCLQNALDILDKVHEPFEDMKCIGLTMQSMYENYIVPEDKREMWM

ACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYRNIEFFTKNSAFPKTT

NGCSQAMAALQNLPQCSPDEIMSYAQKIFKILDEERDKVLTHIDHIFMDILTTCVETMC

NEYKVTSDACMMTMYGGISLLSEFCRVLCCYVLEETSVM LAKRPLITKPEVISVM KR

RIEEICMKVFAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVA YTLATAGASSSDSL

VSPPESPVPATIPLSSVIVAENSDQEESEQSDEEQEEGAQEEREDTVSVKSEPVSEIE EVASEEEEDGAEEPTASGGKSTHPMVTRSKADQ

8 mers:

MESSAKRKjESSAKRKMiSSAKRKMDiSAKRKMDPiAKRKMDPDiKRKMDPDN jRKM

DPDNPjKMDPDNPDiMDPDNPDEjDPDNPDEGiPDNPDEGPiDNPDEGPSiNPDEGP

SSiPDEGPSSKjDEGPSSKViEGPSSKVPiGPSSKVPRiPSSKVPRPiSSKVPRPEjSKV

PRPETjKVPRPETPiVPRPETPViPRPETPVTiRPETPVTKiPETPVTKAiETPVTKATjT

PVTKATT;PVTKATTF;VTKATTFL;TKATTFLQ;KATTFLQT;ATTFLQTM;TTFLQTML;T

FLQTMLR;FLQTMLRK;LQTMLRKE;QTMLRKEV;TMLRKEVN;MLRKEVNS;LRKEVN

SQiRKEVNSQLjKEVNSQLSiEVNSQLSLiVNSQLSLGiNSQLSLGDiSQLSLGDPjQLS

LGDPLjLSLGDPLFiSLGDPLFPiLGDPLFPEiGDPLFPELiDPLFPELAiPLFPELAEjLFP

ELAEEjFPELAEESiPELAEESLiELAEESLKiLAEESLKTiAEESLKTFiEESLKTFEjESL

KTFEQiSLKTFEQVjLKTFEQVTiKTFEQVTEiTFEQVTEDiFEQVTEDCiEQVTEDCNjQ

VTEDCNEiVTEDCNENjTEDCNENPiEDCNENPEiDCNENPEKiCNENPEKDjNENPE

KDVjENPEKDVLiNPEKDVLTiPEKDVLTEiEKDVLTELjKDVLTELViDVLTELVKiVLTE

LVKQiLTELVKQIjTELVKQIKiELVKQIKViLVKQIKVRiVKQIKVRViKQIKVRVDjQIKVR

VDMilKVRVDMVjKVRVDMVRiVRVDMVRHiRVDMVRHRiVDMVRHRIiDMVRHRIKjM

VRHRIKEiVRHRIKEHiRHRIKEHMiHRIKEHMLiRIKEHMLKiIKEHMLKKjKEHMLKKY;

EHMLKKYT;HMLKKYTQ;MLKKYTQT;LKKYTQTE;KKYTQTEE;KYTQTEEK;YTQTEE

KF;TQTEEKFT;QTEEKFTG;TEEKFTGA;EEKFTGAF;EKFTGAFN;KFTGAFNM;FTGA

FNMMjTGAFNMMGiGAFNMMGGiAFNMMGGCiFNMMGGCLjNMMGGCLQiMMGG

CLQNiMGGCLQNAiGGCLQNALiGCLQNALDiCLQNALDIiLQNALDILiQNALDILDjNA

LDILDKiALDILDKViLDILDKVHiDILDKVHEiILDKVHEPiLDKVHEPFiDKVHEPFEiKVH

EPFEDiVHEPFEDMiHEPFEDMKiEPFEDMKCiPFEDMKCIiFEDMKCIGiEDMKCIGL;

DMKCIGLTiMKCIGLTMiKCIGLTMQiCIGLTMQSilGLTMQSMiGLTMQSMYiLTMQSM

YEiTMQSMYENiMQSMYENYiQSMYENYIiSMYENYIViMYENYIVPiYENYIVPEiENYI

VPEDiNYIVPEDKiYIVPEDKRiIVPEDKREiVPEDKREMiPEDKREMWiEDKREMWMiD

KREMWMAiKREMWMACiREMWMACIiEMWMACIKiMWMACIKEiWMACIKELiMACI

KELHiACIKELHDiCIKELHDViIKELHDVSiKELHDVSKiELHDVSKGiLHDVSKGAiHDV

SKGAA;DVSKGAAN ;VSKGAANK;SKGAANKL;KGAANKLG;GAANKLGG;AANKLGG

A;ANKLGGAL;NKLGGALQ;KLGGALQA;LGGALQAK;GGALQAKA;GALQAKAR;ALQA

KARA;LQAKARAK;QAKARAKK;AKARAKKD;KARAKKDE;ARAKKDEL;RAKKDELR;A

KKDELRR;KKDELRRK;KDELRRKM;DELRRKMM;ELRRKMMY;LRRKMMYM;RRKM

MYMCiRKMMYMCYiKMMYMCYRiMMYMCYRNiMYMCYRNIiYMCYRNIEiMCYRNIE

FiCYRNIEFFiYRNIEFFTiRNIEFFTKiNIEFFTKNiIEFFTKNSiEFFTKNSAiFFTKNSAF;

FTKNSAFPiTKNSAFPKiKNSAFPKTiNSAFPKTTiSAFPKTTNiAFPKTTNGiFPKTTNG

C;PKTTNGCS;KTTNGCSQ;TTNGCSQA;TNGCSQAM;NGCSQAMA;GCSQAMAA;CS

QAMAAL;SQAMAALQ;QAMAALQN;AMAALQNL;MAALQNLP;AALQNLPQ;ALQNLPQ

CiLQNLPQCSiQNLPQCSPiNLPQCSPDiLPQCSPDEiPQCSPDEIiQCSPDEIMiCSPD

ElMSiSPDEIMSYiPDEIMSYAjDEIMSYAQjEIMSYAQKilMSYAQKIiMSYAQKIFiSYAQ O-.

KIFKiYAQKIFKIiAQKIFKILiQKIFKILDiKIFKILDEiIFKILDEEiFKILDEERiKILDEERDiIL

DEERDKiLDEERDKViDEERDKVLiEERDKVLTiERDKVLTHiRDKVLTHIiDKVLTHIDiK

VLTHIDHiVLTHIDHIiLTHIDHIFiTHIDHIFMiHIDH IFMDiIDHIFMDIiDHIFMDILiHIFMDIL

TiIFMDILTTiFMDILTTCiMDILTTCViDILTTCVEiILTTCVETiLTTCVETMiTTCVETMC;

TCVETMCNiCVETMCNEiVETMCNEYiETMCNEYKiTMCNEYKViMCNEYKVTiCNEY

KVTSiNEYKVTSDiEYKVTSDAiYKVTSDACiKVTSDACMiVTSDACMMiTSDACMMT;

SDACMMTMiDACMMTMYiACMMTMYGiCMMTMYGGiMMTMYGGhMTMYGGISiTM

YGGISLiMYGGISLLiYGGISLLSiGGISLLSEiGISLLSEFilSLLSEFCiSLLSEFCRiLLSE

FCRViLSEFCRVLiSEFCRVLCiEFCRVLCCiFCRVLCCYiCRVLCCYViRVLCCYVLiVL

CCYVLE;LCCYVLEE;CCYVLEET;CYVLEETS;YVLEETSV;VLEETSVM;LEETSVML;E

ETSVMLAiETSVMLAKiTSVMLAKRiSVMLAKRPiVMLAKRPLiMLAKRPLIiLAKRPLIT;

AKRPLITKiKRPLITKPiRPLITKPEiPLITKPEViLITKPEVIiITKPEVISiTKPEVISViKPEVI

SVMiPEVISVMKiEVISVMKRiVISVMKRRiISVMKRRIiSVMKRRIEiVMKRRIEEiMKRRI

EEIiKRRIEEICiRRIEEICMiRIEEICMKiIEEICMKViEEICMKVFiEICMKVFAiICMKVFA

Q ;CM KVFAQY;M KVFAQYI ;KVFAQYI L;VFAQYILG ;FAQYILGA;AQYI LGAD ;QYI LGAD

PiYILGADPLiILGADPLRiLGADPLRViGADPLRVCiADPLRVCSiDPLRVCSPiPLRVCS

PSiLRVCSPSViRVCSPSVDiVCSPSVDDiCSPSVDDLiSPSVDDLRiPSVDDLRAiSVD

DLRAIiVDDLRAIAiDDLRAIAEiDLRAIAEEiLRAIAEESiRAIAEESDiAIAEESDEiIAEES

DEEiAEESDEEEiEESDEEEAiESDEEEAIiSDEEEAIViDEEEAIVAiEEEAIVAYiEEAIV

AYTiEAIVAYTLiAIVAYTLAiIVAYTLATiVAYTLATAiAYTLATAGiYTLATAGAiTLATAG

ASiLATAGASSiATAGASSSiTAGASSSDiAGASSSDSiGASSSDSLiASSSDSLViSSS

DSLVSiSSDSLVSPiSDSLVSPPiDSLVSPPEiSLVSPPESiLVSPPESPiVSPPESPViS

PPESPVPiPPESPVPAiPESPVPATiESPVPATIiSPVPATIPiPVPATIPLiVPATIPLSiPA

TIPLSSiATIPLSSViTIPLSSVIiIPLSSVIViPLSSVIVAiLSSVIVAEiSSVIVAENiSVIVAEN

SiVIVAENSDiIVAENSDQiVAENSDQEiAENSDQEEiENSDQEESiNSDQEESEiSDQE

ESEQ;DQEESEQS;QEESEQSD;EESEQSDE;ESEQSDEE;SEQSDEEQ;EQSDEEQE

;QSDEEQEE;SDEEQEEG;DEEQEEGA;EEQEEGAQ;EQEEGAQE;QEEGAQEE;EEG

AQEER;EGAQEERE;GAQEERED;AQEEREDT;QEEREDTV;EEREDTVS;EREDTVS

ViREDTVSVKiEDTVSVKSiDTVSVKSEiTVSVKSEPiVSVKSEPViSVKSEPVSiVKSEP

VSEiKSEPVSEIiSEPVSEIEiEPVSEIEEiPVSEIEEViVSEIEEVAiSEIEEVASiEIEEVAS

EiIEEVASEEiEEVASEEEiEVASEEEEiVASEEEEDiASEEEEDGiSEEEEDGAiEEEED

GAE;EEEDGAEE;EEDGAEEP;EDGAEEPT;DGAEEPTA;GAEEPTAS;AEEPTASG;EE

PTASGGiEPTASGGKiPTASGGKSiTASGGKSTiASGGKSTHiSGGKSTHPiGGKSTH

PMiGKSTHPMViKSTHPMVTiSTHPMVTRiTHPMVTRSiHPMVTRSKiPMVTRSKAiMV

TRSKADiVTRSKADQiTRSKADQLiRSKADQLPiSKADQLPGiKADQLPGPiADQLPGP

CiDQLPGPCIiQLPGPCIAiLPGPCIASiPGPCIASTiGPCIASTPiPCIASTPKiCIASTPKK

;IASTPKKH;ASTPKKHR;STPKKHRG;

9 ΓΠΘΓS^"

MESSAKRKMiESSAKRKMDiSSAKRKMDPiSAKRKMDPDiAKRKMDPDNiKRKMDPD

NPiRKMDPDNPDiKMDPDNPDEiMDPDNPDEGiDPDNPDEGPiPDNPDEGPSiDNPD

EGPSSiNPDEGPSSKiPDEGPSSKViDEGPSSKVPiEGPSSKVPRiGPSSKVPRPiPSS

KVPRPEiSSKVPRPETiSKVPRPETPiKVPRPETPViVPRPETPVTiPRPETPVTKiRPE

TPVTKA;PETPVTKAT;ETPVTKATT;TPVTKATTF;PVTKATTFL;VTKATTFLQ;TKATTF

LQT;KATTFLQTM ;ATTFLQTML;TTFLQTMLR;TFLQTMLRK;FLQTMLRKE;LQTMLRK

EViQTMLRKEVNiTMLRKEVNSiMLRKEVNSQiLRKEVNSQLiRKEVNSQLSiKEVNSQ

LSL;EVNSQLSLG;VNSQLSLGD;NSQLSLGDP;SQLSLGDPL;QLSLGDPLF;LSLGDPL

FPiSLGDPLFPEiLGDPLFPELiGDPLFPELAiDPLFPELAEiPLFPELAEEiLFPELAEES;

FPELAEESLiPELAEESLKiELAEESLKTiLAEESLKTFiAEESLKTFEiEESLKTFEQiESL

KTFEQViSLKTFEQVTiLKTFEQVTEiKTFEQVTEDiTFEQVTEDCiFEQVTEDCNiEQV

TEDCNE;QVTEDCNEN;VTEDCNENP;TEDCNENPE;EDCNENPEK;DCNENPEKD;C

NENPEKDViNENPEKDVLiENPEKDVLTiNPEKDVLTEiPEKDVLTELiEKDVLTELViKD

VLTELVKiDVLTELVKQiVLTELVKQIiLTELVKQIKiTELVKQIKViELVKQIKVRiLVKQIK

VRViVKQIKVRVDiKQIKVRVDMiQIKVRVDMViIKVRVDMVRiKVRVDMVRHiVRVDM

VRHRiRVDMVRHRIiVDMVRHRIKiDMVRHRIKEiMVRHRIKEHiVRHRIKEHMiRHRIK

EHML;HRIKEHMLK;RIKEHMLKK;IKEHMLKKY;KEHMLKKYT;EHMLKKYTQ;HMLKKY

TQT;MLKKYTQTE;LKKYTQTEE;KKYTQTEEK;KYTQTEEKF;YTQTEEKFT;TQTEEKF

TG;QTEEKFTGA;TEEKFTGAF;EEKFTGAFN;EKFTGAFNM;KFTGAFNMM;FTGAFN O

MMG;TGAFNMMGG;GAFNMMGGC;AFNMMGGCL;FNMMGGCLQ;NMMGGCLQN;M

MGGCLQNAiMGGCLQNALiGGCLQNALDiGCLQNALDIiCLQNALDILiLQNALDILDiQ

NALDILDKjNALDILDKViALDILDKVHiLDILDKVHEiDILDKVHEPilLDKVHEPFjLDKVH

EPFEiDKVHEPFEDjKVHEPFEDMiVHEPFEDMKjHEPFEDMKCiEPFEDMKCliPFED

MKCIGiFEDMKCIGLjEDMKCIGLTiDMKCIGLTMjMKCIGLTMQiKCIGLTMQSiCIGLT

MQSMiIGLTMQSMYiGLTMQSMYEiLTMQSMYEN TMQSMYENYiMQSMYENYIiQS

MYENYIViSMYENYIVPjMYENYIVPEiYENYIVPEDiENYIVPEDKiNYIVPEDKRjYIVPE

DKREilVPEDKREMjVPEDKREMWiPEDKREMWMiEDKREMWMAjDKREMWMACiK

REMWMACIiREMWMACIKiEMWMACIKEiMWMACIKELiWMACIKELHiMACIKELHD;

ACIKELHDViCIKELHDVSiIKELHDVSKiKELHDVSKGiELHDVSKGAiLHDVSKGAAiH

DVSKGAANiDVSKGAAN KiVSKGAAN KL;SKGAAN KLG;KGAANKLGG;GAANKLGGA

;AANKLGGAL;ANKLGGALQ;NKLGGALQA;KLGGALQAK;LGGALQAKA;GGALQAKA

R;GALQAKARA;ALQAKARAK;LQAKARAKK;QAKARAKKD;AKARAKKDE;KARAKKD

EL;ARAKKDELR;RAKKDELRR;AKKDELRRK;KKDELRRKM;KDELRRKMM;DELRRK

MMYiELRRKMMYMiLRRKMMYMCiRRKMMYMCYiRKMMYMCYRiKMMYMCYRNiM

MYMCYRNIiMYMCYRN IEiYMCYRNIEFiMCYRNIEFFiCYRNIEFFTiYRNIEFFTKiRNI

EFFTKNiNIEFFTKNSiIEFFTKNSAiEFFTKNSAFiFFTKNSAFPiFTKNSAFPKiTKNSAF

PKTiKNSAFPKTTiNSAFPKTTNiSAFPKTTNGiAFPKTTNGCiFPKTTNGCSiPKTTNG

CSQiKTTNGCSQAiTTNGCSQAMiTNGCSQAMAiNGCSQAMAAiGCSQAMAALiCSQ

AMAALQiSQAMAALQNiQAMAALQNLiAMAALQNLPiMAALQNLPQiAALQNLPQCiAL

QNLPQCSiLQNLPQCSPiQNLPQCSPDiNLPQCSPDEiLPQCSPDEIiPQCSPDEIMiQ

CSPDEIMSiCSPDEIMSYiSPDEIMSYAiPDEIMSYAQiDEIMSYAQKiEIMSYAQKIilMS

YAQKIFiMSYAQKIFKiSYAQKIFKIiYAQKIFKILiAQKIFKILDiQKIFKILDEiKIFKILDEEiI

FKILDEERiFKILDEERDiKILDEERDKiILDEERDKViLDEERDKVLiDEERDKVLTiEER

DKVLTHiERDKVLTHIiRDKVLTHIDiDKVLTHIDHiKVLTHIDHIiVLTHIDHIFiLTHIDH IF

MiTHIDHIFMDiHIDHIFMDIiIDHIFMDILiDHIFMDILTiHIFMDILTTiIFMDILTTCiFMDILT

TCViMDILTTCVEiDILTTCVETiILTTCVETMiLTTCVETMCiTTCVETMCNiTCVETMCN

EiCVETMCNEYiVETMCNEYKiETMCNEYKViTMCNEYKVTiMCNEYKVTSiCNEYKV

TSDiNEYKVTSDAiEYKVTSDACiYKVTSDACMiKVTSDACMMiVTSDACMMTiTSDAC

MMTMiSDACMMTMYiDACMMTMYGiACMMTMYGGiCMMTMYGGIiMMTMYGGIS;

MTMYGGISLTMYGGISLLiMYGGISLLSiYGGISLLSEiGGISLLSEFiGISLLSEFCiISLL

SEFCRiSLLSEFCRViLLSEFCRVLiLSEFCRVLCiSEFCRVLCCiEFCRVLCCYiFCRVL

CCYViCRVLCCYVLiRVLCCYVLEiVLCCYVLEEiLCCYVLEETiCCYVLEETSiCYVLEE

TSViYVLEETSVMiVLEETSVMLiLEETSVMLAiEETSVMLAKiETSVMLAKRiTSVMLAK

RPiSVMLAKRPLiVMLAKRPLIiMLAKRPLITiLAKRPLITKiAKRPLITKPiKRPLITKPEiR

PLITKPEViPLITKPEVIiLITKPEVISiITKPEVISViTKPEVISVMiKPEVISVMKiPEVISVM

KRiEVISVMKRRiVISVMKRRIiISVMKRRIEiSVMKRRIEEiVMKRRIEEIiMKRRIEEICiK

RRIEEICMiRRIEEICMKiRIEEICMKViIEEICMKVFiEEICMKVFAiEICMKVFAQiICMKV

FAQYiCMKVFAQYIiMKVFAQYILiKVFAQYILGiVFAQYILGAiFAQYILGADiAQYILGA

DPiQYILGADPLiYILGADPLRiILGADPLRViLGADPLRVCiGADPLRVCSiADPLRVCS

PiDPLRVCSPSiPLRVCSPSViLRVCSPSVDiRVCSPSVDDiVCSPSVDDLiCSPSVDDL

RiSPSVDDLRAiPSVDDLRAIiSVDDLRAIAiVDDLRAIAEiDDLRAIAEEiDLRAIAEESiL

RAIAEESDiRAIAEESDEiAIAEESDEEiIAEESDEEEiAEESDEEEAiEESDEEEAIiESD

EEEAIViSDEEEAIVAiDEEEAIVAYiEEEAIVAYTiEEAIVAYTLiEAIVAYTLAiAIVAYTLA

TiIVAYTLATAiVAYTLATAGiAYTLATAGAiYTLATAGASiTLATAGASSiLATAGASSSiA

TAGASSSDiTAGASSSDSiAGASSSDSLiGASSSDSLViASSSDSLVSiSSSDSLVSPiS

SDSLVSPPiSDSLVSPPEiDSLVSPPESiSLVSPPESPiLVSPPESPViVSPPESPVPiSP

PESPVPAiPPESPVPATiPESPVPATIiESPVPATIPiSPVPATIPLiPVPATIPLSiVPATIP

LSSiPATIPLSSViATIPLSSVIiTIPLSSVIViIPLSSVIVAiPLSSVIVAEiLSSVIVAENiSSVI

VAENSiSVIVAENSDiVIVAENSDQiIVAENSDQEiVAENSDQEEiAENSDQEESiENSD

QEESEiNSDQEESEQiSDQEESEQSiDQEESEQSDiQEESEQSDEiEESEQSDEEiES

EQSDEEQ;SEQSDEEQE;EQSDEEQEE;QSDEEQEEG;SDEEQEEGA;DEEQEEGAQ

;EEQEEGAQE;EQEEGAQEE;QEEGAQEER;EEGAQEERE;EGAQEERED;GAQEER

EDTiAQEEREDTViQEEREDTVSiEEREDTVSViEREDTVSVKiREDTVSVKSiEDTVS

VKSEiDTVSVKSEPiTVSVKSEPViVSVKSEPVSiSVKSEPVSEiVKSEPVSEIiKSEPVS

EIEiSEPVSEIEEiEPVSEIEEViPVSEIEEVAiVSEIEEVASiSEIEEVASEiEIEEVASEEiI

EEVASEEEiEEVASEEEEiEVASEEEEDiVASEEEEDGiASEEEEDGAiSEEEEDGAE;

EEEEDGAEE;EEEDGAEEP;EEDGAEEPT;EDGAEEPTA;DGAEEPTAS;GAEEPTAS GiAEEPTASGGjEEPTASGGKiEPTASGGKSiPTASGGKSTiTASGGKSTH jASGGKST

HPiSGGKSTHPMjGGKSTHPMViGKSTHPMVTjKSTHPMVTRiSTHPMVTRSiTHPMV

TRSKjHPMVTRSKAiPMVTRSKADiMVTRSKADQiVTRSKADQLjTRSKADQLPiRSKA

DQLPGiSKADQLPGPiKADQLPGPCiADQLPGPCIiDQLPGPCIAiQLPG PCIAS;LPGP

CIASTjPGPCIASTPiGPCIASTPKiPCIASTPKKiCIASTPKKHilASTPKKHRjASTPKKH

RG;

10 mers:

MESSAKRKMDjESSAKRKMDPiSSAKRKMDPDiSAKRKMDPDNiAKRKMDPDNPjKR

KMDPDNPDjRKMDPDNPDEjKMDPDNPDEGiMDPDNPDEGPiDPDNPDEGPSiPDN

PDEGPSSiDNPDEGPSSKjNPDEGPSSKViPDEGPSSKVPiDEGPSSKVPRjEGPSSK

VPRPiGPSSKVPRPEjPSSKVPRPETiSSKVPRPETPiSKVPRPETPVjKVPRPETPVT;

VPRPETPVTK;PRPETPVTKA;RPETPVTKAT;PETPVTKATT;ETPVTKATTF;TPVTKA

TTFL;PVTKATTFLQ;VTKATTFLQT;TKATTFLQTM;KATTFLQTML;ATTFLQTMLR;TT

FLQTMLRK;TFLQTMLRKE;FLQTMLRKEV;LQTMLRKEVN;QTMLRKEVNS;TMLRKE

VNSQiMLRKEVNSQLjLRKEVNSQLSiRKEVNSQLSLjKEVNSQLSLGiEVNSQLSLGD

;VNSQLSLGDP;NSQLSLGDPL;SQLSLGDPLF;QLSLGDPLFP;LSLGDPLFPE;SLGDP

LFPELjLGDPLFPELAiGDPLFPELAEiDPLFPELAEEiPLFPELAEESiLFPELAEESLjFP

ELAEESLKiPELAEESLKTiELAEESLKTFjLAEESLKTFEjAEESLKTFEQiEESLKTFEQ

VjESLKTFEQVTiSLKTFEQVTEiLKTFEQVTEDiKTFEQVTEDCiTFEQVTEDCNjFEQV

TEDCNEjEQVTEDCNENiQVTEDCNENPiVTEDCNENPEiTEDCNENPEKjEDCNENP

EKDjDCNENPEKDViCNENPEKDVLiNENPEKDVLTiENPEKDVLTEjNPEKDVLTELiP

EKDVLTELViEKDVLTELVKjKDVLTELVKQiDVLTELVKQliVLTELVKQIKjLTELVKQIK

ViTELVKQIKVRjELVKQIKVRViLVKQIKVRVDiVKQIKVRVDMjKQIKVRVDMViQIKVR

VDMVRilKVRVDMVRHjKVRVDMVRHRiVRVDMVRHRIjRVDMVRHRIKiVDMVRHRI

KEiDMVRHRIKEHjMVRHRIKEHMiVRHRIKEHMLiRHRIKEHMLKiHRIKEHMLKKjRIK

EHMLKKY;IKEHMLKKYT;KEHMLKKYTQ;EHMLKKYTQT;HMLKKYTQTE;MLKKYTQ

TEE;LKKYTQTEEK;KKYTQTEEKF;KYTQTEEKFT;YTQTEEKFTG;TQTEEKFTGA;QT

EEKFTGAF;TEEKFTGAFN;EEKFTGAFNM;EKFTGAFNMM;KFTGAFNMMG;FTGAF

NMMGG;TGAFNMMGGC;GAFNMMGGCL;AFNMMGGCLQ;FNMMGGCLQN;NMMG

GCLQNAjMMGGCLQNALjMGGCLQNALDiGGCLQNALDIiGCLQNALDILiCLQNALDI

LDjLQNALDILDKiQNALDILDKViNALDILDKVHiALDILDKVHEjLDILDKVHEPiDILDKV

HEPFilLDKVHEPFEiLDKVHEPFEDjDKVHEPFEDMjKVHEPFEDMKiVHEPFEDMKC;

HEPFEDMKCliEPFEDMKCIGiPFEDMKCIGLjFEDMKCIGLTiEDMKCIGLTMjDMKCI

GLTMQiMKCIGLTMQSiKCIGLTMQSMjCIGLTMQSMYilGLTMQSMYEjGLTMQSMY

ENiLTMQSMYENYjTMQSMYENYIiMQSMYENYIViQSMYENYIVPiSMYENYIVPEjM

YENYlVPEDiYENYlVPEDKiENYlVPEDKRjNYlVPEDKREjYlVPEDKREMilVPEDKRE

MWiVPEDKREMWMjPEDKREMWMAjEDKREMWMACiDKREMWMACliKREMWMA

CIKiREMWMACIKEjEMWMACIKELiMWMACIKELHiWMACIKELHDjMACIKELHDViA

CIKELHDVSiCIKELHDVSKilKELHDVSKGjKELHDVSKGAiELHDVSKGAAjLHDVSKG

AAN jHDVSKGAANKjDVSKGAANKLiVSKGAANKLGiSKGAANKLGGiKGAANKLGGA

;GAANKLGGAL;AANKLGGALQ;ANKLGGALQA;NKLGGALQAK;KLGGALQAKA;LGG

ALQAKAR;GGALQAKARA;GALQAKARAK;ALQAKARAKK;LQAKARAKKD;QAKARA

KKDE;AKARAKKDEL;KARAKKDELR;ARAKKDELRR;RAKKDELRRK;AKKDELRRKM jKKDELRRKMMiKDELRRKMMYiDELRRKMMYMjELRRKMMYMCiLRRKMMYMCY;

RRKMMYMCYRiRKMMYMCYRNjKMMYMCYRNIiMMYMCYRNIEiMYMCYRN IEFjY

MCYRNIEFFiMCYRNIEFFTiCYRNIEFFTKjYRN IEFFTKNjRNIEFFTKNSiNIEFFTKNS

AilEFFTKNSAFjEFFTKNSAFPiFFTKNSAFPKiFTKNSAFPKTiTKNSAFPKTTjKNSAF

PKTTNjNSAFPKTTNGiSAFPKTTNGCiAFPKTTNGCSjFPKTTNGCSQiPKTTNGCSQ

AiKTTNGCSQAMiTTNGCSQAMAjTNGCSQAMAAjNGCSQAMAALiGCSQAMAALQ;

CSQAMAALQNiSQAMAALQNLiQAMAALQNLPjAMAALQNLPQjMAALQNLPQCiAAL

QNLPQCS ;ALQNLPQCSP;LQNLPQCSPD;QNLPQCSPDE;NLPQCSPDEI;LPQCSPD

ElMjPQCSPDEIMSiQCSPDEIMSYiCSPDEIMSYAiSPDEIMSYAQiPDEIMSYAQKjDE

IMSYAQKIjEIMSYAQKIFjIMSYAQKIFKiMSYAQKIFKIiSYAQKIFKILiYAQKIFKILDjAQ

KIFKILDEiQKIFKILDEEjKIFKILDEERilFKILDEERDiFKILDEERDKjKILDEERDKVilLD

EERDKVLiLDEERDKVLTiDEERDKVLTHjEERDKVLTHIiERDKVLTHIDjRDKVLTHID

HiDKVLTHIDHIjKVLTHIDHIFiVLTHIDHIFMiLTHIDHIFMDjTHIDHIFMDIiHIDHIFMDIL;

IDHIFMDILTiDHIFMDILTTiHIFMDILTTCilFMDILTTCViFMDILTTCVEjMDILTTCVET; DILTTCVETMilLTTCVETMCiLTTCVETMCNjTTCVETMCNEjTCVETMCNEYiCVETM

CNEYK;VETMCNEYKV;ETMCNEYKVT;TMCNEYKVTS;MCNEYKVTSD;CNEYKVTS

DAiNEYKVTSDACiEYKVTSDACMjYKVTSDACMMjKVTSDACMMTiVTSDACMMTM;

TSDACMMTMYiSDACMMTMYGiDACMMTMYGGiACMMTMYGGIiCMMTMYGGIS;

MMTMYGGISLiMTMYGGISLLjTMYGGISLLSiMYGGISLLSEjYGGISLLSEFiGGISLLS

EFCiGISLLSEFCRjISLLSEFCRViSLLSEFCRVLjLLSEFCRVLCiLSEFCRVLCCiSEFC

RVLCCYiEFCRVLCCYVjFCRVLCCYVLiCRVLCCYVLEjRVLCCYVLEEiVLCCYVLEE

TjLCCYVLEETSiCCYVLEETSViCYVLEETSVMiYVLEETSVMLiVLEETSVMLAjLEET

SVMLAKiEETSVMLAKRjETSVMLAKRPjTSVMLAKRPLiSVMLAKRPLIiVM LAKRPLIT jMLAKRPLITKiLAKRPLITKPiAKRPLITKPEjKRPLITKPEViRPLITKPEVIiPLITKPEVIS;

LITKPEVISVilTKPEVISVMiTKPEVISVMKjKPEVISVMKRiPEVISVMKRRjEVISVMKR

RliVISVMKRRIEjlSVMKRRIEEiSVMKRRIEEliVMKRRIEEICiMKRRIEEICMjKRRIEEI

CMKjRRIEEICMKViRIEEICMKVFilEEICMKVFAjEEICMKVFAQiEICMKVFAQYilCMK

VFAQYIiCMKVFAQYILjMKVFAQYILGiKVFAQYILGAiVFAQYILGADiFAQYILGADPjA

QYILGADPLiQYILGADPLRjYILGADPLRVjILGADPLRVCiLGADPLRVCSiGADPLRV

CSPjADPLRVCSPSiDPLRVCSPSViPLRVCSPSVDiLRVCSPSVDDjRVCSPSVDDLiV

CSPSVDDLRiCSPSVDDLRAiSPSVDDLRAIjPSVDDLRAIAiSVDDLRAIAEjVDDLRAI

AEEjDDLRAIAEESiDLRAIAEESDiLRAIAEESDEiRAIAEESDEEjAIAEESDEEEilAEE

SDEEEAjAEESDEEEAIjEESDEEEAIVjESDEEEAIVAiSDEEEAIVAYiDEEEAIVAYTjE

EEAIVA YTL;EEAIVAYTLA;EAIVA YTLAT ;AIVA YTLATA; IVA YTLAT AG;VA YTLAT AGA;

AYTLATAGASiYTLATAGASSiTLATAGASSSiLATAGASSSDiATAGASSSDSiTAGAS

SSDSLiAGASSSDSLViGASSSDSLVSiASSSDSLVSPiSSSDSLVSPPiSSDSLVSPPE

;SDSLVSPPES;DSLVSPPESP;SLVSPPESPV;LVSPPESPVP;VSPPESPVPA;SPPES

PVPATiPPESPVPATIiPESPVPATIPiESPVPATIPLiSPVPATIPLSiPVPATIPLSSiVPA

TIPLSSViPATIPLSSVIiATIPLSSVIViTIPLSSVIVAiIPLSSVIVAEiPLSSVIVAENiLSSVI

VAENSiSSVIVAENSDiSVIVAENSDQiVIVAENSDQEiIVAENSDQEEiVAENSDQEES;

AENSDQEESEiENSDQEESEQiNSDQEESEQSiSDQEESEQSDiDQEESEQSDEiQE

ESEQSDEE;EESEQSDEEQ;ESEQSDEEQE;SEQSDEEQEE;EQSDEEQEEG;QSDE

EQEEGA;SDEEQEEGAQ;DEEQEEGAQE;EEQEEGAQEE;EQEEGAQEER;QEEGAQ

EERE;EEGAQEERED;EGAQEEREDT;GAQEEREDTV;AQEEREDTVS;QEEREDTVS

ViEEREDTVSVKiEREDTVSVKSiREDTVSVKSEiEDTVSVKSEPiDTVSVKSEPViTVS

VKSEPVSiVSVKSEPVSEiSVKSEPVSEIiVKSEPVSEIEiKSEPVSEIEEiSEPVSEIEEV;

EPVSEIEEVAiPVSEIEEVASiVSEIEEVASEiSEIEEVASEEiEIEEVASEEEiIEEVASEE

EEiEEVASEEEEDiEVASEEEEDGiVASEEEEDGAiASEEEEDGAEiSEEEEDGAEEiE

EEEDGAEEPiEEEDGAEEPTiEEDGAEEPTAiEDGAEEPTASiDGAEEPTASGiGAEE

PTASGGiAEEPTASGGKiEEPTASGGKSiEPTASGGKSTiPTASGGKSTHiTASGGKS

THP;ASGGKSTHPM;SGGKSTHPMV;GGKSTHPMVT;GKSTHPMVTR;KSTHPMVTR

SiSTHPMVTRSKiTHPMVTRSKAiHPMVTRSKADiPMVTRSKADQiMVTRSKADQLiVT

RSKADQLPiTRSKADQLPGiRSKADQLPGPiSKADQLPGPCiKADQLPGPCIiADQLPG

PCIAiDQLPGPCIASiQLPGPCIASTiLPGPCIASTPiPGPCIASTPKiGPCIASTPKKiPCI

ASTPKKH;CIASTPKKHR;IASTPKKHRG;

1 1 mers:

MESSAKRKMDPiESSAKRKMDPDiSSAKRKMDPDNiSAKRKMDPDNPiAKRKMDPD

NPDiKRKMDPDNPDEiRKMDPDNPDEGiKMDPDNPDEGPiMDPDNPDEGPSiDPDN

PDEGPSSiPDNPDEGPSSKiDNPDEGPSSKViNPDEGPSSKVPiPDEGPSSKVPRiDE

GPSSKVPRPiEGPSSKVPRPEiGPSSKVPRPETiPSSKVPRPETPiSSKVPRPETPViS

KVPRPETPVTiKVPRPETPVTKiVPRPETPVTKAiPRPETPVTKATiRPETPVTKATTiP

ETPVTKATTF;ETPVTKATTFL;TPVTKATTFLQ;PVTKATTFLQT;VTKATTFLQTM;TKA

TTFLQTML;KATTFLQTMLR;ATTFLQTMLRK;TTFLQTMLRKE;TFLQTMLRKEV;FLQT

MLRKEVNiLQTMLRKEVNSiQTMLRKEVNSQiTMLRKEVNSQLiMLRKEVNSQLSiLR

KEVNSQLSLiRKEVNSQLSLGiKEVNSQLSLGDiEVNSQLSLGDPiVNSQLSLGDPLiN

SQLSLGDPLFiSQLSLGDPLFPiQLSLGDPLFPEiLSLGDPLFPELiSLGDPLFPELAiLG

DPLFPELAEiGDPLFPELAEEiDPLFPELAEESiPLFPELAEESLiLFPELAEESLKiFPEL

AEESLKTiPELAEESLKTFiELAEESLKTFEiLAEESLKTFEQiAEESLKTFEQViEESLKT

FEQVTiESLKTFEQVTEiSLKTFEQVTEDiLKTFEQVTEDCiKTFEQVTEDCNiTFEQVT

EDCNE;FEQVTEDCNEN;EQVTEDCNENP;QVTEDCNENPE;VTEDCNENPEK;TEDC

NENPEKDiEDCNENPEKDVjDCNENPEKDVLiCNENPEKD VLT;NENPEKDVLTE;ENP o

EKDVLTELiNPEKDVLTELViPEKDVLTELVKiEKDVLTELVKQiKDVLTELVKQIiDVLTE

LVKQIKiVLTELVKQIKViLTELVKQIKVRjTELVKQIKVRViELVKQIKVRVDjLVKQIKVR

VDMiVKQIKVRVDMVjKQIKVRVDMVRiQIKVRVDMVRHilKVRVDMVRHRjKVRVDMV

RHRliVRVDMVRHRIKjRVDMVRHRIKEiVDMVRHRIKEHiDMVRHRIKEHMjMVRHRI

KEHML;VRHRIKEHMLK;RHRIKEHMLKK;HRIKEHMLKKY;RIKEHMLKKYT;IKEHMLK

KYTQ;KEHMLKKYTQT;EHMLKKYTQTE;HMLKKYTQTEE;MLKKYTQTEEK;LKKYTQ

TEEKF;KKYTQTEEKFT;KYTQTEEKFTG;YTQTEEKFTGA;TQTEEKFTGAF;QTEEKF

TGAFN;TEEKFTGAFNM;EEKFTGAFNMM;EKFTGAFNMMG;KFTGAFNMMGG;FTG

AFNMMGGC;TGAFNMMGGCL;GAFNMMGGCLQ;AFNMMGGCLQN;FNMMGGCLQ

NAiNMMGGCLQNALjMMGGCLQNALDjMGGCLQNALDIiGGCLQNALDILiGCLQNAL

DILDiCLQNALDILDKjLQNALDILDKViQNALDILDKVHiNALDILDKVHEjALDILDKVHE

PiLDILDKVHEPFjDILDKVHEPFEilLDKVHEPFEDiLDKVHEPFEDMjDKVHEPFEDMK iKVHEPFEDMKCiVHEPFEDMKCIjHEPFEDMKCIGjEPFEDMKCIGLiPFEDMKCIGLT;

FEDMKCIGLTMjEDMKCIGLTMQiDMKCIGLTMQSiMKCIGLTMQSMjKCIGLTMQSM

YiCIGLTMQSMYEiIGLTMQSMYENiGLTMQSMYENYiLTMQSMYENYIiTMQSMYEN

YIVjMQSMYENYIVPiQSMYENYIVPEiSMYENYIVPEDiMYENYIVPEDKjYENYIVPED

KRiENYIVPEDKREjNYIVPEDKREMjYIVPEDKREMWilVPEDKREMWMiVPEDKREM

WMAiPEDKREMWMACiEDKREMWMACIiDKREMWMACIKiKREMWMACIKEiREM

WMACIKELiEMWMACIKELHiMWMACIKELHDiWMACIKELHDViMACIKELHDVSiAC

IKELHDVSKiCIKELHDVSKGiIKELHDVSKGAiKELHDVSKGAAiELHDVSKGAANiLHD

VSKGAANKiHDVSKGAAN KLiDVSKGAAN KLG;VSKGAANKLGG;SKGAAN KLGGAiK

GAANKLGGALiGAAN KLGGALQ;AANKLGGALQA;AN KLGGALQAK;NKLGGALQAKA

;KLGGALQAKAR;LGGALQAKARA;GGALQAKARAK;GALQAKARAKK;ALQAKARAK

KD;LQAKARAKKDE;QAKARAKKDEL;AKARAKKDELR;KARAKKDELRR;ARAKKDEL

RRKiRAKKDELRRKM ;AKKDELRRKMM;KKDELRRKMMY;KDELRRKMMYM;DELRR

KMMYMCiELRRKMMYMCYiLRRKMMYMCYRiRRKMMYMCYRNiRKMMYMCYRNI;

KMMYMCYRNIEiMMYMCYRNIEFiMYMCYRNIEFFiYMCYRNIEFFTiMCYRNIEFFTK;

CYRNIEFFTKNiYRNIEFFTKNSiRNIEFFTKNSAiNIEFFTKNSAFiIEFFTKNSAFPiEFF

TKNSAFPKiFFTKNSAFPKTiFTKNSAFPKTTiTKNSAFPKTTNiKNSAFPKTTNGiNSA

FPKTTNGCiSAFPKTTNGCSiAFPKTTNGCSQiFPKTTNGCSQAiPKTTNGCSQAMiK

TTNGCSQAMAiTTNGCSQAMAAiTNGCSQAMAALiNGCSQAMAALQiGCSQAMAAL

QNiCSQAMAALQNLiSQAMAALQNLPiQAMAALQNLPQiAMAALQNLPQCiMAALQNL

PQCSiAALQNLPQCSPiALQNLPQCSPDiLQNLPQCSPDEiQNLPQCSPDEIiNLPQCS

PDEIMiLPQCSPDEIMSiPQCSPDEIMSYiQCSPDEIMSYAiCSPDEIMSYAQiSPDEIM

SYAQKiPDEIMSYAQKIiDEIMSYAQKIFiEIMSYAQKIFKiIMSYAQKIFKIiMSYAQKIFKI

LiSYAQKIFKILDiYAQKIFKILDEiAQKIFKILDEEiQKIFKILDEERiKIFKILDEERDiIFKIL

DEERDKiFKILDEERDKViKILDEERDKVLiILDEERDKVLTiLDEERDKVLTHiDEERDK

VLTHIiEERDKVLTHIDiERDKVLTHIDHiRDKVLTHIDHIiDKVLTHIDHIFiKVLTHIDHIF

MiVLTHIDHIFMDiLTHIDHIFMDIiTHIDHIFMDILiHIDH IFMDILTiIDHIFMDILTTiDHIFM

DILTTCiHIFMDILTTCViIFMDILTTCVEiFMDILTTCVETiMDILTTCVETMiDILTTCVET

MCiILTTCVETMCNiLTTCVETMCNEiTTCVETMCNEYiTCVETMCNEYKiCVETMCNE

YKViVETMCNEYKVTiETMCNEYKVTSiTMCNEYKVTSDiMCNEYKVTSDAiCNEYKV

TSDACiNEYKVTSDACMiEYKVTSDACMMiYKVTSDACMMTiKVTSDACMMTMiVTS

DACMMTMYiTSDACMMTMYGiSDACMMTMYGGiDACMMTMYGGIiACMMTMYGGI

SiCMMTMYGGISLiMMTMYGGISLLiMTMYGGISLLSiTMYGGISLLSEiMYGGISLLSE

FiYGGISLLSEFCiGGISLLSEFCRiGISLLSEFCRViISLLSEFCRVLiSLLSEFCRVLCiLL

SEFCRVLCCiLSEFCRVLCCYiSEFCRVLCCYViEFCRVLCCYVLiFCRVLCCYVLEiCR

VLCCYVLEEiRVLCCYVLEETiVLCCYVLEETSiLCCYVLEETSViCCYVLEETSVMiCY

VLEETSVMLiYVLEETSVMLAiVLEETSVMLAKiLEETSVMLAKRiEETSVMLAKRPiET

SVMLAKRPLiTSVMLAKRPLIiSVMLAKRPLITiVMLAKRPLITKiMLAKRPLITKPiLAKR

PLITKPEiAKRPLITKPEViKRPLITKPEVIiRPLITKPEVISiPLITKPEVISViLITKPEVISV

MiITKPEVISVMKiTKPEVISVMKRiKPEVISVMKRRiPEVISVMKRRIiEVISVMKRRIEiVI

SVMKRRIEEilSVMKRRIEEliSVMKRRIEEICiVMKRRIEEICMiMKRRIEEICMKiKRRIE

EICMKViRRIEEICMKVFiRIEEICMKVFAiIEEICMKVFAQiEEICMKVFAQYiEICMKVFA

QYIiICMKVFAQYILiCMKVFAQYILGiMKVFAQYILGAiKVFAQYILGADiVFAQYILGAD

PiFAQYILGADPLiAQYILGADPLRiQYILGADPLRViYILGADPLRVCiILGADPLRVCSiL

GADPLRVCSPiGADPLRVCSPSiADPLRVCSPSViDPLRVCSPSVDiPLRVCSPSVDD;

LRVCSPSVDDLiRVCSPSVDDLRiVCSPSVDDLRAiCSPSVDDLRAIiSPSVDDLRAIA; o

PSVDDLRAIAEiSVDDLRAIAEEjVDDLRAIAEESiDDLRAIAEESDiDLRAIAEESDEjLR

AlAEESDEEiRAIAEESDEEEjAIAEESDEEEAjlAEESDEEEAIjAEESDEEEAIVjEESDE

EEAIVAjESDEEEAIVAYiSDEEEAIVAYTiDEEEAIVAYTLjEEEAIVAYTLAjEEAIVAYTL

AT;EAIVAYTLATA;AIVA YTLAT AG;IVAYTLATAGA;VA YTLAT AGAS;AYTLAT AGASS;

YTLAT AGASSSiTLATAGASSSDjLATAGASSSDSjATAGASSSDSLjTAGASSSDSLV;

AGASSSDSLVSiGASSSDSLVSPjASSSDSLVSPPjSSSDSLVSPPEjSSDSLVSPPES;

SDSLVSPPESPiDSLVSPPESPVjSLVSPPESPVPjLVSPPESPVPAjVSPPESPVPAT;

SPPESPVPATIjPPESPVPATIPiPESPVPATIPLjESPVPATIPLSiSPVPATIPLSSiPVP

ATlPLSSViVPATIPLSSVIiPATIPLSSVIVjATIPLSSVIVAjTIPLSSVIVAEjIPLSSVIVAE

NjPLSSVIVAENSiLSSVIVAENSDiSSVIVAENSDQiSVIVAENSDQEjVIVAENSDQEE;!

VAENSDQEESiVAENSDQEESEjAENSDQEESEQjENSDQEESEQSjNSDQEESEQS

DiSDQEESEQSDEjDQEESEQSDEEiQEESEQSDEEQiEESEQSDEEQEjESEQSDE

EQEE;SEQSDEEQEEG;EQSDEEQEEGA;QSDEEQEEGAQ;SDEEQEEGAQE;DEEQ

EEGAQEE;EEQEEGAQEER;EQEEGAQEERE;QEEGAQEERED;EEGAQEEREDT;E

GAQEEREDTVjGAQEEREDTVSiAQEEREDTVSVjQEEREDTVSVKjEEREDTVSVKS jEREDTVSVKSEiREDTVSVKSEPiEDTVSVKSEPVjDTVSVKSEPVSiTVSVKSEPVSE iVSVKSEPVSEIjSVKSEPVSEIEiVKSEPVSEIEEiKSEPVSEIEEViSEPVSEIEEVAjEP

VSEIEEVASiPVSEIEEVASEjVSEIEEVASEEiSEIEEVASEEEjEIEEVASEEEEilEEVA

SEEEEDjEEVASEEEEDGjEVASEEEEDGAjVASEEEEDGAEjASEEEEDGAEEiSEEE

EDGAEEPjEEEEDGAEEPTjEEEDGAEEPTAjEEDGAEEPTASjEDGAEEPTASGiDG

AEEPTASGGiGAEEPTASGGKjAEEPTASGGKSjEEPTASGGKSTjEPTASGGKSTH;

PTASGGKSTHPjTASGGKSTHPMjASGGKSTHPMViSGGKSTHPMVTjGGKSTHPMV

TRjGKSTHPMVTRSiKSTHPMVTRSKiSTHPMVTRSKAiTHPMVTRSKADjHPMVTRS

KADQiPMVTRSKADQLjMVTRSKADQLPΛ/TRSKADQLPGiTRSKADQLPGPiRSKAD

QLPGPCiSKADQLPGPCIiKADQLPGPCIAiADQLPGPCIASiDQLPGPCIASTiQLPGP

CIASTPiLPGPCIASTPKiPGPCIASTPKKiGPCIASTPKKHiPCIASTPKKHRiCIASTPKK

HRG;

13 mers:

MESSAKRKMDPDNiESSAKRKMDPDNPiSSAKRKMDPDNPDiSAKRKMDPDNPDE;

AKRKMDPDNPDEGiKRKMDPDNPDEGPiRKMDPDNPDEGPSiKMDPDNPDEGPSS;

MDPDNPDEGPSSKiDPDNPDEGPSSKViPDNPDEGPSSKVPiDNPDEGPSSKVPRiN

PDEGPSSKVPRPiPDEGPSSKVPRPEiDEGPSSKVPRPETiEGPSSKVPRPETPiGPS

SKVPRPETPViPSSKVPRPETPVTiSSKVPRPETPVTKiSKVPRPETPVTKAiKVPRPE

TPVTKATiVPRPETPVTKATTiPRPETPVTKATTFiRPETPVTKATTFLiPETPVTKATTF

LQ;ETPVTKATTFLQT ;TPVTKATTFLQTM;PVTKATTFLQTML;VTKATTFLQTMLR;TK

ATTFLQTMLRK;KATTFLQTMLRKE ;ATTFLQTMLRKEV;TTFLQTMLRKEVN ;TFLQTM

LRKEVNSiFLQTMLRKEVNSQiLQTMLRKEVNSQLiQTMLRKEVNSQLSiTMLRKEVN

SQLSLiMLRKEVNSQLSLGiLRKEVNSQLSLGDiRKEVNSQLSLGDPiKEVNSQLSLG

DPLiEVNSQLSLGDPLFiVNSQLSLGDPLFPiNSQLSLGDPLFPEiSQLSLGDPLFPEL;

QLSLGDPLFPELAiLSLGDPLFPELAEiSLGDPLFPELAEEiLGDPLFPELAEESiGDPLF

PELAEESLiDPLFPELAEESLKiPLFPELAEESLKTiLFPELAEESLKTFiFPELAEESLKT

FEiPELAEESLKTFEQiELAEESLKTFEQViLAEESLKTFEQVTiAEESLKTFEQVTEiEE

SLKTFEQVTEDiESLKTFEQVTEDCiSLKTFEQVTEDCNiLKTFEQVTEDCNEiKTFEQ

VTEDCNENiTFEQVTEDCNENPiFEQVTEDCNENPEiEQVTEDCNENPEKiQVTEDC

NENPEKDiVTEDCNENPEKDViTEDCNENPEKDVLiEDCNENPEKDVLTiDCNENPEK

DVLTEiCNENPEKDVLTELiNENPEKDVLTELViENPEKDVLTELVKiNPEKDVLTELVK

QiPEKDVLTELVKQIiEKDVLTELVKQIKiKDVLTELVKQIKViDVLTELVKQIKVRiVLTEL

VKQIKVRViLTELVKQIKVRVDiTELVKQIKVRVDMiELVKQIKVRVDMViLVKQIKVRVD

MVRiVKQIKVRVDMVRHiKQIKVRVDMVRHRiQIKVRVDMVRHRIiIKVRVDMVRHRIK;

KVRVDMVRHRIKEiVRVDMVRHRIKEHiRVDMVRHRIKEHMiVDMVRHRIKEHMLiDM

VRHRIKEHMLKiMVRHRIKEHMLKKiVRHRIKEHMLKKYiRHRIKEHMLKKYTiHRIKEH

MLKKYTQ;RIKEHMLKKYTQT;IKEHMLKKYTQTE;KEHMLKKYTQTEE;EHMLKKYTQ

TEEK;HMLKKYTQTEEKF;MLKKYTQTEEKFT;LKKYTQTEEKFTG;KKYTQTEEKFTG

A;KYTQTEEKFTGAF;YTQTEEKFTGAFN;TQTEEKFTGAFNM;QTEEKFTGAFNMM;T

EEKFTGAFNMMGiEEKFTGAFNMMGGiEKFTGAFNMMGGCiKFTGAFNMMGGCLiF

TGAFNMMGGCLQiTGAFNMMGGCLQNiGAFNMMGGCLQNAiAFNMMGGCLQNAL;

FNMMGGCLQNALDiNMMGGCLQNALDIiMMGGCLQNALDILjMGGCLQNALDILDiG o

GCLQNALDILDKiGCLQNALDILDKVjCLQNALDILDKVHjLQNALDILDKVHEiQNALDIL

DKVHEPiNALDILDKVHEPFjALDILDKVHEPFEjLDILDKVHEPFEDjDILDKVHEPFED

MjILDKVHEPFEDMKjLDKVHEPFEDMKCiDKVHEPFEDMKCliKVHEPFEDMKCIGiV

HEPFEDMKCIGLiHEPFEDMKCIGLTjEPFEDMKCIGLTMjPFEDMKCIGLTMQiFEDM

KCIGLTMQSiEDMKCIGLTMQSMiDMKCIGLTMQSMYjMKCIGLTMQSMYEjKCIGLT

MQSMYENiCIGLTMQSMYENYjlGLTMQSMYENYIiGLTMQSMYENYIVjLTMQSMYE

NYIVPjTMQSMYENYIVPEjMQSMYENYIVPEDiQSMYENYIVPEDKjSMYENYIVPED

KRiMYENYIVPEDKREjYENYIVPEDKREMjENYIVPEDKREMWjNYIVPEDKREMWM;

YIVPEDKREMWMAjlVPEDKREMWMACiVPEDKREMWMACliPEDKREMWMACIKjE

DKREMWMACIKEiDKREMWMACIKELiKREMWMACIKELHjREMWMACIKELHDjEM

WMACIKELHDVjMWMACIKELHDVSiWMACIKELHDVSKjMACIKELHDVSKGiACIKE

LHDVSKGAiCIKELHDVSKGAAilKELHDVSKGAANjKELHDVSKGAANKjELHDVSKG

AANKLjLHDVSKGAANKLGiHDVSKGAANKLGGjDVSKGAANKLGGAjVSKGAANKLG

GAL;SKGAANKLGGALQ;KGAANKLGGALQA;GAANKLGGALQAK;AAN KLGGALQAK

A;ANKLGGALQAKAR;NKLGGALQAKARA;KLGGALQAKARAK;LGGALQAKARAKK;

GGALQAKARAKKD;GALQAKARAKKDE;ALQAKARAKKDEL;LQAKARAKKDELR;QA

KARAKKDELRR;AKARAKKDELRRK;KARAKKDELRRKM;ARAKKDELRRKMM;RAKK

DELRRKMMYjAKKDELRRKMMYMiKKDELRRKMMYMCiKDELRRKMMYMCYjDELR

RKMMYMCYRiELRRKMMYMCYRNjLRRKMMYMCYRNIjRRKMMYMCYRNIEjRKM

MYMCYRNIEFiKMMYMCYRNIEFFjMMYMCYRNIEFFTjMYMCYRNIEFFTKjYMCYR

NIEFFTKNiMCYRNIEFFTKNSiCYRNIEFFTKNSAiYRNIEFFTKNSAFiRNIEFFTKNSA

FPiNIEFFTKNSAFPKiIEFFTKNSAFPKTiEFFTKNSAFPKTTiFFTKNSAFPKTTNiFTK

NSAFPKTTNGiTKNSAFPKTTNGCiKNSAFPKTTNGCSiNSAFPKTTNGCSQiSAFPKT

TNGCSQAiAFPKTTNGCSQAMiFPKTTNGCSQAMAiPKTTNGCSQAMAAiKTTNGCS

QAMAALiTTNGCSQAMAALQiTNGCSQAMAALQNiNGCSQAMAALQNLiGCSQAMA

ALQNLP;CSQAMAALQNLPQ;SQAMAALQNLPQC ;QAMAALQNLPQCS;AMAALQNL

PQCSPiMAALQNLPQCSPDiAALQNLPQCSPDEiALQNLPQCSPDEIiLQNLPQCSPD

ElMiQNLPQCSPDEIMSiNLPQCSPDEIMSYiLPQCSPDEIMSYAiPQCSPDEIMSYAQ;

QCSPDEIMSYAQKiCSPDEIMSYAQKIiSPDEIMSYAQKIFiPDEIMSYAQKIFKiDEIMS

YAQKIFKIiEIMSYAQKIFKILiIMSYAQKIFKILDiMSYAQKIFKILDEiSYAQKIFKILDEEiY

AQKIFKILDEERiAQKIFKILDEERDiQKIFKILDEERDKiKIFKILDEERDKViIFKILDEER

DKVLiFKILDEERDKVLTiKILDEERDKVLTHiILDEERDKVLTHIiLDEERDKVLTHIDiDE

ERDKVLTHIDHiEERDKVLTHIDHIiERDKVLTHIDHIFiRDKVLTHIDHIFMiDKVLTHIDH

IFMDiKVLTHIDHIFMDIiVLTHIDHIFMDILiLTHIDHIFMDILTiTHIDHIFMDILTTiHIDHIF

MDILTTCiIDHIFMDILTTCViDHIFMDILTTCVEiHIFMDILTTCVETiIFMDILTTCVETMiF

MDILTTCVETMCiMDILTTCVETMCNiDILTTCVETMCNEiILTTCVETMCNEYiLTTCVE

TMCNEYKiTTCVETMCNEYKViTCVETMCNEYKVTiCVETMCNEYKVTSiVETMCNEY

KVTSD;ETMCNEYKVTSDA;TMCNEYKVTSDAC;MCNEYKVTSDACM;CNEYKVTSD

ACMMiNEYKVTSDACMMTiEYKVTSDACMMTMiYKVTSDACMMTMYiKVTSDACMM

TMYGiVTSDACMMTMYGGiTSDACMMTMYGGIiSDACMMTMYGGISiDACMMTMYG

GISLiACMMTMYGGISLLiCMMTMYGGISLLSiMMTMYGGISLLSEiMTMYGGISLLSE

FiTMYGGISLLSEFCiMYGGISLLSEFCRiYGGISLLSEFCRViGGISLLSEFCRVLiGISL

LSEFCRVLCiISLLSEFCRVLCCiSLLSEFCRVLCCYiLLSEFCRVLCCYViLSEFCRVLC

CYVLiSEFCRVLCCYVLEiEFCRVLCCYVLEEiFCRVLCCYVLEETiCRVLCCYVLEETS

;RVLCCYVLEETSV;VLCCYVLEETSVM;LCCYVLEETSVML;CCYVLEETSVMLA;CYV

LEETSVMLAKiYVLEETSVMLAKRiVLEETSVMLAKRPiLEETSVMLAKRPLiEETSVML

AKRPLIiETSVMLAKRPLITiTSVMLAKRPLITKiSVMLAKRPLITKPiVMLAKRPLITKPE;

MLAKRPLITKPEViLAKRPLITKPEVIiAKRPLITKPEVISiKRPLITKPEVISViRPLITKPEV

ISVMiPLITKPEVISVMKiLITKPEVISVMKRiITKPEVISVMKRRiTKPEVISVMKRRIiKPE

VISVMKRRIEiPEVISVMKRRIEEiEVISVMKRRIEEIiVISVMKRRIEEICiISVMKRRIEEI

CMiSVMKRRIEEICMKiVMKRRIEEICMKViMKRRIEEICMKVFiKRRIEEICMKVFAiRRI

EEICMKVFAQiRIEEICMKVFAQYiIEEICMKVFAQYIiEEICMKVFAQYILiEICMKVFAQ

YILGiICMKVFAQYILGAiCMKVFAQYILGADiMKVFAQYILGADPiKVFAQYILGADPLiV

FAQYILGADPLRiFAQYILGADPLRViAQYILGADPLRVCiQYILGADPLRVCSiYILGAD

PLRVCSPiILGADPLRVCSPSiLGADPLRVCSPSViGADPLRVCSPSVDiADPLRVCSP

SVDDiDPLRVCSPSVDDLiPLRVCSPSVDDLRiLRVCSPSVDDLRAiRVCSPSVDDLR

AIiVCSPSVDDLRAIAiCSPSVDDLRAIAEiSPSVDDLRAIAEEiPSVDDLRAIAEESiSVD

DLRAIAEESDiVDDLRAIAEESDEiDDLRAIAEESDEEiDLRAIAEESDEEEiLRAIAEES DEEEAjRAIAEESDEEEAIiAIAEESDEEEAIVjIAEESDEEEAIVAjAEESDEEEAIVAYjE

ESDEEEAIVA YTjESDEEEAIVAYTLiSDEEEAIVAYTLAjDEEEAIVAYTLATjEEEAIVAY

TLATA;EEAIVA YTLAT AG;EAIVA YTLAT AGA;AIVA YTLAT AGAS;IVA YTLAT AGASS;V

AYTLAT AGASSSiAYTLATAGASSSDjYTLATAGASSSDSjTLATAGASSSDSLjLAT AG

ASSSDSLVjATAGASSSDSLVSiTAGASSSDSLVSPjAGASSSDSLVSPPjGASSSDSL

VSPPEjASSSDSLVSPPESiSSSDSLVSPPESPjSSDSLVSPPESPVjSDSLVSPPESP

VPiDSLVSPPESPVPAiSLVSPPESPVPATjLVSPPESPVPATIjVSPPESPVPATIPjSP

PESPVPATIPLjPPESPVPATIPLSiPESPVPATIPLSSjESPVPATIPLSSVjSPVPATIPL

SSVIiPVPATIPLSSVIViVPATIPLSSVIVAjPATIPLSSVIVAEiATIPLSSVIVAENjTIPLSS

VIVAENSilPLSSVIVAENSDjPLSSVIVAENSDQjLSSVIVAENSDQEjSSVIVAENSDQE

EiSVIVAENSDQEESiVIVAENSDQEESEjlVAENSDQEESEQiVAENSDQEESEQSjAE

NSDQEESEQSDjENSDQEESEQSDEjNSDQEESEQSDEEjSDQEESEQSDEEQiDQ

EESEQSDEEQEiQEESEQSDEEQEEjEESEQSDEEQEEGjESEQSDEEQEEGAjSEQ

SDEEQEEGAQ;EQSDEEQEEGAQE;QSDEEQEEGAQEE;SDEEQEEGAQEER;DEE

QEEGAQEERE;EEQEEGAQEERED;EQEEGAQEEREDT;QEEGAQEEREDTV;EEG

AQEEREDTVSjEGAQEEREDTVSVjGAQEEREDTVSVKjAQEEREDTVSVKSiQEER

EDTVSVKSEjEEREDTVSVKSEPjEREDTVSVKSEPVjREDTVSVKSEPVSiEDTVSVK

SEPVSEjDTVSVKSEPVSEIjTVSVKSEPVSEIEjVSVKSEPVSEIEEiSVKSEPVSEIEE

ViVKSEPVSEIEEVAjKSEPVSEIEEVASiSEPVSEIEEVASEiEPVSEIEEVASEEjPVSEI

EEVASEEEjVSEIEEVASEEEEjSEIEEVASEEEEDjEIEEVASEEEEDGilEEVASEEEE

DGAjEEVASEEEEDGAEjEVASEEEEDGAEEiVASEEEEDGAEEPjASEEEEDGAEEP

TiSEEEEDGAEEPTAjEEEEDGAEEPTASiEEEDGAEEPTASGiEEDGAEEPTASGGjE

DGAEEPTASGGKjDGAEEPTASGGKSiGAEEPTASGGKSTjAEEPTASGGKSTHjEE

PTASGGKSTHPiEPTASGGKSTHPMjPTASGGKSTHPMVjTASGGKSTHPMVTjASG

GKSTHPMVTRjSGGKSTHPMVTRSiGGKSTHPMVTRSKjGKSTHPMVTRSKAjKSTH

PMVTRSKADjSTHPMVTRSKADQiTHPMVTRSKADQLjHPMVTRSKADQLPjPMVTR

SKADQLPGiMVTRSKADQLPGPjVTRSKADQLPGPCiTRSKADQLPGPCIjRSKADQL

PGPCIAiSKADQLPGPCIASiKADQLPGPCIASTjADQLPGPCIASTPjDQLPG PCIASTP

KiQLPGPCIASTPKKjLPGPCIASTPKKHjPGPCIASTPKKHRiG PCIASTPKKHRG;

14 mers:

MESSAKRKMDPDNPjESSAKRKMDPDNPDjSSAKRKMDPDNPDEjSAKRKMDPDNP

DEGjAKRKMDPDNPDEGPjKRKMDPDNPDEGPSiRKMDPDNPDEGPSSjKMDPDNP

DEGPSSKjMDPDNPDEGPSSKViDPDNPDEGPSSKVPjPDNPDEGPSSKVPRjDNPD

EGPSSKVPRPiNPDEGPSSKVPRPEjPDEGPSSKVPRPETjDEGPSSKVPRPETPjEG

PSSKVPRPETPVjGPSSKVPRPETPVTjPSSKVPRPETPVTKiSSKVPRPETPVTKAjS

KVPRPETPVTKATjKVPRPETPVTKATTiVPRPETPVTKATTFjPRPETPVTKATTFLjR

PETPVTKATTFLQiPETPVTKATTFLQTjETPVTKATTFLQTMjTPVTKATTFLQTMLjPV

TKATTFLQTMLR;VTKATTFLQTMLRK;TKATTFLQTMLRKE;KATTFLQTMLRKEV;AT

TFLQTMLRKEVNjTTFLQTMLRKEVNSjTFLQTMLRKEVNSQiFLQTMLRKEVNSQLjL

QTMLRKEVNSQLSjQTMLRKEVNSQLSLjTMLRKEVNSQLSLGjMLRKEVNSQLSLG

DiLRKEVNSQLSLGDPjRKEVNSQLSLGDPLjKEVNSQLSLGDPLFjEVNSQLSLGDPL

FPjVNSQLSLGDPLFPEjNSQLSLGDPLFPELjSQLSLGDPLFPELAiQLSLGDPLFPEL

AEjLSLGDPLFPELAEEjSLGDPLFPELAEESiLGDPLFPELAEESLjGDPLFPELAEESL

KiDPLFPELAEESLKTjPLFPELAEESLKTFjLFPELAEESLKTFEjFPELAEESLKTFEQ;

PELAEESLKTFEQVjELAEESLKTFEQVTjLAEESLKTFEQVTEjAEESLKTFEQVTED;

EESLKTFEQVTEDCjESLKTFEQVTEDCNjSLKTFEQVTEDCNEjLKTFEQVTEDCNE

NiKTFEQVTEDCNENPjTFEQVTEDCNENPEjFEQVTEDCNENPEKjEQVTEDCNENP

EKDiQVTEDCNENPEKDVjVTEDCNENPEKDVLjTEDCNENPEKDVLTjEDCNENPEK

DVLTEjDCNENPEKDVLTELiCNENPEKDVLTELVjNENPEKDVLTELVKjENPEKDVLT

ELVKQiNPEKDVLTELVKQIjPEKDVLTELVKQIKjEKDVLTELVKQIKVjKDVLTELVKQI

KVRiDVLTELVKQIKVRVjVLTELVKQIKVRVDjLTELVKQIKVRVDMjTELVKQIKVRVD

MViELVKQIKVRVDMVRjLVKQIKVRVDMVRHjVKQIKVRVDMVRHRjKQIKVRVDMVR

HRliQIKVRVDMVRHRIKjlKVRVDMVRHRIKEjKVRVDMVRHRIKEHjVRVDMVRHRIK

EHMiRVDMVRHRIKEHMLjVDMVRHRIKEHMLKjDMVRHRIKEHMLKKjMVRHRIKEH

MLKKYjVRHRIKEHMLKKYTjRHRIKEHMLKKYTQiHRIKEHMLKKYTQTjRIKEHMLKK

YTQTE;IKEHMLKKYTQTEE;KEHMLKKYTQTEEK;EHMLKKYTQTEEKF;HMLKKYTQ

TEEKFT;MLKKYTQTEEKFTG;LKKYTQTEEKFTGA;KKYTQTEEKFTGAF;KYTQTEE KFTGAFN;YTQTEEKFTGAFNM;TQTEEKFTGAFNMM;QTEEKFTGAFNMMG;TEEKF

TGAFNMMGGiEEKFTGAFNMMGGCiEKFTGAFNMMGGCLiKFTGAFNMMGGCLQiF

TGAFNMMGGCLQNjTGAFNMMGGCLQNAiGAFNMMGGCLQNALjAFNMMGGCLQ

NALDiFNMMGGCLQNALDIiNMMGGCLQNALDILiMMGGCLQNALDILDiMGGCLQN

ALDILDKiGGCLQNALDILDKViGCLQNALDILDKVHiCLQNALDILDKVHEiLQNALDILD

KVHEPiQNALDILDKVHEPFiNALDILDKVHEPFEiALDILDKVHEPFEDiLDILDKVHEPF

EDMiDILDKVHEPFEDMKiILDKVHEPFEDMKCiLDKVHEPFEDMKCIiDKVHEPFEDM

KCIGiKVHEPFEDMKCIGLiVHEPFEDMKCIGLTiHEPFEDMKCIGLTMiEPFEDMKCIG

LTMQiPFEDMKCIGLTMQSiFEDMKCIGLTMQSMiEDMKCIGLTMQSMYiDMKCIGLT

MQSMYEiMKCIGLTMQSMYENiKCIGLTMQSMYENYiCIGLTMQSMYENYIiIGLTMQ

SMYENYIViGLTMQSMYENYIVPiLTMQSMYENYIVPEiTMQSMYENYIVPEDiMQSM

YENYIVPEDKiQSMYENYIVPEDKRiSMYENYIVPEDKREiMYENYIVPEDKREMiYEN

YIVPEDKREMWiENYIVPEDKREMWMiNYIVPEDKREMWMAiYIVPEDKREMWMACiI

VPEDKREMWMACIiVPEDKREMWMACIKiPEDKREMWMACIKEiEDKREMWMACIK

ELiDKREMWMACIKELHiKREMWMACIKELHDiREMWMACIKELHDViEMWMACIKE

LHDVSiMWMACIKELHDVSKiWMACIKELHDVSKGiMACIKELHDVSKGAiACIKELHD

VSKGAAiCIKELHDVSKGAANiIKELHDVSKGAANKiKELHDVSKGAANKLiELHDVSK

GAANKLG;LHDVSKGAANKLGG;HDVSKGAANKLGGA;DVSKGAANKLGGAL;VSKG

AAN KLGGALQ;SKGAANKLGGALQA;KGAAN KLGGALQAK;GAANKLGGALQAKA;AA

NKLGGALQAKAR ;ANKLGGALQAKARA;NKLGGALQAKARAK;KLGGALQAKARAKK;

LGGALQAKARAKKD;GGALQAKARAKKDE;GALQAKARAKKDEL;ALQAKARAKKDE

LR;LQAKARAKKDELRR;QAKARAKKDELRRK;AKARAKKDELRRKM;KARAKKDELR

RKMM;ARAKKDELRRKMMY;RAKKDELRRKMMYM;AKKDELRRKMMYMC;KKDELR

RKMMYMCYiKDELRRKMMYMCYRiDELRRKMMYMCYRNiELRRKMMYMCYRNIiLR

RKMMYMCYRNIEiRRKMMYMCYRNIEFiRKMMYMCYRNIEFFiKMMYMCYRNIEFFT;

MMYMCYRNIEFFTKiMYMCYRNIEFFTKNiYMCYRNIEFFTKNSiMCYRNIEFFTKNSA

;CYRNIEFFTKNSAF;YRNIEFFTKNSAFP;RNIEFFTKNSAFPK;NIEFFTKNSAFPKT;IE

FFTKNSAFPKTTiEFFTKNSAFPKTTNiFFTKNSAFPKTTNGiFTKNSAFPKTTNGCiTK

NSAFPKTTNGCSiKNSAFPKTTNGCSQiNSAFPKTTNGCSQAiSAFPKTTNGCSQAM;

AFPKTTNGCSQAMAiFPKTTNGCSQAMAAiPKTTNGCSQAMAALiKTTNGCSQAMA

ALQiTTNGCSQAMAALQN iTNGCSQAMAALQNLiNGCSQAMAALQNLPiGCSQAMA

ALQNLPQiCSQAMAALQNLPQCiSQAMAALQNLPQCSiQAMAALQNLPQCSPiAMAA

LQNLPQCSPDiMAALQNLPQCSPDEiAALQNLPQCSPDEIiALQNLPQCSPDEIMiLQN

LPQCSPDEIMSiQNLPQCSPDEIMSYiNLPQCSPDEIMSYAiLPQCSPDEIMSYAQiPQ

CSPDEIMSYAQKiQCSPDEIMSYAQKI ;CSPDEIMSYAQKIF;SPDEIMSYAQKIFK;PDE

IMSYAQKIFKIiDEIMSYAQKIFKILiEIMSYAQKIFKILDiIMSYAQKIFKILDEiMSYAQKIF

KILDEEiSYAQKIFKILDEERiYAQKIFKILDEERDiAQKIFKILDEERDKiQKIFKILDEERD

KViKIFKILDEERDKVLiIFKILDEERDKVLTiFKILDEERDKVLTHiKILDEERDKVLTHIiIL

DEERDKVLTHIDiLDEERDKVLTHIDHiDEERDKVLTHIDHIiEERDKVLTHIDHIFiERDK

VLTHIDHIFMiRDKVLTHIDHIFMDiDKVLTHIDHIFMDIiKVLTHIDHIFMDILiVLTHIDHIF

MDILTiLTHIDHIFMDILTT iTHIDHIFMDILTTCiHIDHIFMDILTTCVilDHIFMDILTTCVEiD

HIFMDILTTCVETiHIFMDILTTCVETMiIFMDILTTCVETMCiFMDILTTCVETMCNiMDIL

TTCVETMCNEiDILTTCVETMCNEYiILTTCVETMCNEYKiLTTCVETMCNEYKViTTCV

ETMCNEYKVT;TCVETMCNEYKVTS;CVETMCNEYKVTSD;VETMCNEYKVTSDA;ET

MCNEYKVTSDAC;TMCNEYKVTSDACM;MCNEYKVTSDACMM;CNEYKVTSDACMM

TiNEYKVTSDACMMTMiEYKVTSDACMMTMYiYKVTSDACMMTMYGiKVTSDACMM

TMYGGiVTSDACMMTMYGGIiTSDACMMTMYGGISiSDACMMTMYGGISLiDACMMT

MYGGISLLiACMMTMYGGISLLSiCMMTMYGGISLLSEiMMTMYGGISLLSEFiMTMY

GGISLLSEFCiTMYGGISLLSEFCRiMYGGISLLSEFCRViYGGISLLSEFCRVLiGGISL

LSEFCRVLCiGISLLSEFCRVLCCiISLLSEFCRVLCCYiSLLSEFCRVLCCYViLLSEFC

RVLCCYVLiLSEFCRVLCCYVLEiSEFCRVLCCYVLEEiEFCRVLCCYVLEETiFCRVLC

CYVLEETSiCRVLCCYVLEETSViRVLCCYVLEETSVMiVLCCYVLEETSVMLiLCCYVL

EETSVMLAiCCYVLEETSVMLAKiCYVLEETSVMLAKRiYVLEETSVMLAKRPiVLEET

SVMLAKRPLiLEETSVMLAKRPLIiEETSVMLAKRPLITiETSVMLAKRPLITKiTSVMLA

KRPLITKPiSVMLAKRPLITKPEiVMLAKRPLITKPEViMLAKRPLITKPEVIiLAKRPLITK

PEVISiAKRPLITKPEVISViKRPLITKPEVISVMiRPLITKPEVISVMKiPLITKPEVISVMK

RiLITKPEVISVMKRRiITKPEVISVMKRRIiTKPEVISVMKRRIEiKPEVISVMKRRIEEiPE

VISVM KRRIEEliEVISVMKRRIEEICiVISVMKRRIEEICMilSVMKRRIEEICMKiSVM KR RIEEICMKViVMKRRIEEICMKVFjMKRRIEEICMKVFAjKRRIEEICMKVFAQiRRIEEIC

MKVFAQYjRIEEICMKVFAQYIilEEICMKVFAQYILjEEICMKVFAQYILGiEICMKVFAQ

YILGAilCMKVFAQYILGADiCMKVFAQYILGADPjMKVFAQYILGADPLjKVFAQYILGA

DPLRiVFAQYILGADPLRViFAQYILGADPLRVCiAQYILGADPLRVCSiQYILGADPLRV

CSPiYILGADPLRVCSPSilLGADPLRVCSPSVjLGADPLRVCSPSVDjGADPLRVCSPS

VDDiADPLRVCSPSVDDLjDPLRVCSPSVDDLRiPLRVCSPSVDDLRAjLRVCSPSVD

DLRAIjRVCSPSVDDLRAIAjVCSPSVDDLRAIAEiCSPSVDDLRAIAEEiSPSVDDLRAI

AEESiPSVDDLRAIAEESDjSVDDLRAIAEESDEjVDDLRAIAEESDEEjDDLRAIAEESD

EEEjDLRAIAEESDEEEAjLRAIAEESDEEEAIiRAIAEESDEEEAIVjAIAEESDEEEAIVA jIAEESDEEEAIVAYjAEESDEEEAIVAYTjEESDEEEAIVAYTLjESDEEEAIVAYTLAiSD

EEEAIVA YTLATjDEEEAIVAYTLATAjEEEAIVAYTLATAGiEEAIVAYTLATAGAjEAIVA

YTLAT AGAS;AIVAYTLATAGASS;IVA YTLAT AG ASSS; VA YTLATAG ASSS D; AYTLAT

AGASSSDSiYTLATAGASSSDSLjTLATAGASSSDSLVjLATAGASSSDSLVSjATAGA

SSSDSLVSPjTAGASSSDSLVSPPjAGASSSDSLVSPPEjGASSSDSLVSPPESiASSS

DSLVSPPESPiSSSDSLVSPPESPVjSSDSLVSPPESPVPjSDSLVSPPESPVPAjDSL

VSPPESPVPATiSLVSPPESPVPATIiLVSPPESPVPATIPiVSPPESPVPATIPLiSPPES

PVPATIPLSiPPESPVPATIPLSSiPESPVPATIPLSSViESPVPATIPLSSVIiSPVPATIPL

SSVIViPVPATIPLSSVIVAjVPATIPLSSVIVAEjPATIPLSSVIVAENjATIPLSSVIVAENS;

TIPLSSVIVAENSDjIPLSSVIVAENSDQiPLSSVIVAENSDQEjLSSVIVAENSDQEEjSS

VIVAENSDQEESiSVIVAENSDQEESEjVIVAENSDQEESEQjlVAENSDQEESEQSjVA

ENSDQEESEQSDjAENSDQEESEQSDEjENSDQEESEQSDEEjNSDQEESEQSDEE

QiSDQEESEQSDEEQEjDQEESEQSDEEQEEjQEESEQSDEEQEEGjEESEQSDEE

QEEGA;ESEQSDEEQEEGAQ;SEQSDEEQEEGAQE;EQSDEEQEEGAQEE;QSDEE

QEEGAQEER;SDEEQEEGAQEERE;DEEQEEGAQEERED;EEQEEGAQEEREDT;E

QEEGAQEEREDTV;QEEGAQEEREDTVS;EEGAQEEREDTVSV;EGAQEEREDTVS

VKjGAQEEREDTVSVKSiAQEEREDTVSVKSEjQEEREDTVSVKSEPjEEREDTVSVK

SEPViEREDTVSVKSEPVSiREDTVSVKSEPVSEiEDTVSVKSEPVSEIiDTVSVKSEP

VSEIEiTVSVKSEPVSEIEEiVSVKSEPVSEIEEViSVKSEPVSEIEEVAiVKSEPVSEIEE

VASiKSEPVSEIEEVASEiSEPVSEIEEVASEEiEPVSEIEEVASEEEiPVSEIEEVASEE

EEiVSEIEEVASEEEEDiSEIEEVASEEEEDGiEIEEVASEEEEDGAiIEEVASEEEEDGA

EiEEVASEEEEDGAEEiEVASEEEEDGAEEPiVASEEEEDGAEEPTiASEEEEDGAEE

PTAiSEEEEDGAEEPTASiEEEEDGAEEPTASGiEEEDGAEEPTASGGiEEDGAEEPT

ASGGK;EDGAEEPTASGGKS;DGAEEPTASGGKST;GAEEPTASGGKSTH;AEEPTA

SGGKSTHPiEEPTASGGKSTHPMiEPTASGGKSTHPMViPTASGGKSTHPMVTiTAS

GGKSTHPMVTRiASGGKSTHPMVTRSiSGGKSTHPMVTRSKiGGKSTHPMVTRSKA;

GKSTHPMVTRSKADiKSTHPMVTRSKADQiSTHPMVTRSKADQLiTHPMVTRSKADQ

LPiHPMVTRSKADQLPGiPMVTRSKADQLPGPiMVTRSKADQLPGPCiVTRSKADQL

PGPCIiTRSKADQLPGPCIAiRSKADQLPGPCIASiSKADQLPGPCIASTiKADQLPGPC

IASTPiADQLPG PCIASTPK;DQLPGPCIASTPKK;QLPG PCIASTPKKH iLPG PCIASTP

KKHRiPGPCIASTPKKHRG;

15 mers:

MESSAKRKMDPDNPDiESSAKRKMDPDNPDEiSSAKRKMDPDNPDEGiSAKRKMDP

DNPDEGPiAKRKMDPDNPDEGPSiKRKMDPDNPDEGPSSiRKMDPDNPDEGPSSK;

KMDPDNPDEGPSSKViMDPDNPDEGPSSKVPiDPDNPDEGPSSKVPRiPDNPDEGP

SSKVPRPiDNPDEGPSSKVPRPEiNPDEGPSSKVPRPETiPDEGPSSKVPRPETPiDE

GPSSKVPRPETPViEGPSSKVPRPETPVTiGPSSKVPRPETPVTKiPSSKVPRPETPV

TKAiSSKVPRPETPVTKATiSKVPRPETPVTKATTiKVPRPETPVTKATTFiVPRPETPV

TKATTFLiPRPETPVTKATTFLQiRPETPVTKATTFLQTiPETPVTKATTFLQTMiETPVT

KATTFLQTML;TPVTKATTFLQTMLR;PVTKATTFLQTMLRK;VTKATTFLQTMLRKE;T

KATTFLQTMLRKEV;KATTFLQTMLRKEVN ;ATTFLQTMLRKEVNS;TTFLQTMLRKEV

NSQiTFLQTMLRKEVNSQLiFLQTMLRKEVNSQLSiLQTMLRKEVNSQLSLiQTMLRK

EVNSQLSLGiTMLRKEVNSQLSLGDiMLRKEVNSQLSLGDPiLRKEVNSQLSLGDPL;

RKEVNSQLSLGDPLFiKEVNSQLSLGDPLFPiEVNSQLSLGDPLFPEiVNSQLSLGDPL

FPELiNSQLSLGDPLFPELAiSQLSLGDPLFPELAEiQLSLGDPLFPELAEEiLSLGDPLF

PELAEESiSLGDPLFPELAEESLiLGDPLFPELAEESLKiGDPLFPELAEESLKTiDPLFP

ELAEESLKTFiPLFPELAEESLKTFEiLFPELAEESLKTFEQiFPELAEESLKTFEQViPE

LAEESLKTFEQVT;ELAEESLKTFEQVTE;LAEESLKTFEQVTED;AEESLKTFEQVTED CiEESLKTFEQVTEDCNjESLKTFEQVTEDCNEiSLKTFEQVTEDCNENjLKTFEQVTE

DCNENPiKTFEQVTEDCNENPEiTFEQVTEDCNENPEKiFEQVTEDCNENPEKDjEQV

TEDCNENPEKDViQVTEDCNENPEKD VLiVTEDCNENPEKD VLTiTEDCNENPEKDVL

TEiEDCNENPEKDVLTELiDCNENPEKDVLTELViCNENPEKDVLTELVKiNENPEKDV

LTELVKQiENPEKDVLTELVKQIiNPEKDVLTELVKQIKiPEKDVLTELVKQIKViEKDVLT

ELVKQIKVRiKDVLTELVKQIKVRViDVLTELVKQIKVRVDiVLTELVKQIKVRVDMiLTEL

VKQIKVRVDMViTELVKQIKVRVDMVRiELVKQIKVRVDMVRHiLVKQIKVRVDMVRHR iVKQIKVRVDMVRHRliKQIKVRVDMVRHRIKiQIKVRVDMVRHRIKEilKVRVDMVRHRI

KEH iKVRVDMVRHRIKEHMiVRVDMVRHRIKEHMLiRVDMVRHRIKEHMLKiVDMVRH

RIKEHMLKKiDMVRHRIKEHMLKKYiMVRHRIKEHMLKKYTiVRHRIKEHMLKKYTQiR

HRIKEHMLKKYTQT;HRIKEHMLKKYTQTE;RIKEHMLKKYTQTEE;IKEHMLKKYTQTE

EK;KEHMLKKYTQTEEKF;EHMLKKYTQTEEKFT;HMLKKYTQTEEKFTG;MLKKYTQT

EEKFTGA;LKKYTQTEEKFTGAF;KKYTQTEEKFTGAFN;KYTQTEEKFTGAFNM;YTQ

TEEKFTGAFNMM;TQTEEKFTGAFNMMG;QTEEKFTGAFNMMGG;TEEKFTGAFNM

MGGCiEEKFTGAFNMMGGCLiEKFTGAFNMMGGCLQiKFTGAFNMMGGCLQNiFTG

AFNMMGGCLQNAiTGAFNMMGGCLQNALiGAFNMMGGCLQNALDiAFNMMGGCLQ

NALDIiFNMMGGCLQNALDILiNMMGGCLQNALDILDiMMGGCLQNALDILDKiMGGC

LQNALDILDKViGGCLQNALDILDKVHiGCLQNALDILDKVHEiCLQNALDILDKVHEPiL

QNALDILDKVHEPFiQNALDILDKVHEPFEiNALDILDKVHEPFEDiALDILDKVHEPFED

MiLDILDKVHEPFEDMKiDILDKVHEPFEDMKCiILDKVHEPFEDMKCIiLDKVHEPFED

MKCIGiDKVHEPFEDMKCIGLiKVHEPFEDMKCIGLTiVHEPFEDMKCIGLTMiHEPFE

DMKCIGLTMQiEPFEDMKCIGLTMQSiPFEDMKCIGLTMQSMiFEDMKCIGLTMQSMY iEDMKCIGLTMQSMYEiDMKCIGLTMQSMYENiMKCIGLTMQSMYENYiKCIGLTMQS

MYENYIiCIGLTMQSMYENYIViIGLTMQSMYENYIVPiGLTMQSMYENYIVPEiLTMQS

MYENYIVPEDiTMQSMYENYIVPEDKiMQSMYENYIVPEDKRiQSMYENYIVPEDKRE;

SMYENYIVPEDKREMiMYENYIVPEDKREMWiYENYIVPEDKREMWMiENYIVPEDK

REMWMAiNYIVPEDKREMWMACiYIVPEDKREMWMACIiIVPEDKREMWMACIKiVP

EDKREMWMACIKEiPEDKREMWMACIKELiEDKREMWMACIKELHiDKREMWMACI

KELHDiKREMWMACIKELHDViREMWMACIKELHDVSiEMWMACIKELHDVSKiMWM

ACIKELHDVSKGiWMACIKELHDVSKGAiMACIKELHDVSKGAAiACIKELHDVSKGAA

NiCIKELHDVSKGAANKiIKELHDVSKGAANKLiKELHDVSKGAANKLGiELHDVSKGA

ANKLGG;LHDVSKGAANKLGGA;HDVSKGAANKLGGAL;DVSKGAANKLGGALQ;VS

KGAANKLGGALQA;SKGAANKLGGALQAK;KGAANKLGGALQAKA;GAANKLGGALQ

AKARiAAN KLGGALQAKARA;ANKLGGALQAKARAK;NKLGGALQAKARAKK;KLGGA

LQAKARAKKD;LGGALQAKARAKKDE;GGALQAKARAKKDEL;GALQAKARAKKDEL

R;ALQAKARAKKDELRR;LQAKARAKKDELRRK;QAKARAKKDELRRKM;AKARAKKD

ELRRKMM;KARAKKDELRRKMMY;ARAKKDELRRKMMYM;RAKKDELRRKMMYMC;

AKKDELRRKMMYMCYiKKDELRRKMMYMCYRiKDELRRKMMYMCYRNiDELRRKM

MYMCYRNIiELRRKMMYMCYRNIEiLRRKMMYMCYRNIEFiRRKMMYMCYRNIEFFiR

KMMYMCYRNIEFFTiKMMYMCYRNIEFFTKiMMYMCYRNIEFFTKNiMYMCYRNIEFF

TKNSiYMCYRNIEFFTKNSAiMCYRNIEFFTKNSAFiCYRNIEFFTKNSAFPiYRNIEFFT

KNSAFPKiRNIEFFTKNSAFPKTiNIEFFTKNSAFPKTTiIEFFTKNSAFPKTTNiEFFTKN

SAFPKTTNGiFFTKNSAFPKTTNGCiFTKNSAFPKTTNGCSiTKNSAFPKTTNGCSQiK

NSAFPKTTNGCSQAiNSAFPKTTNGCSQAMiSAFPKTTNGCSQAMAiAFPKTTNGCS

QAMAAiFPKTTNGCSQAMAALiPKTTNGCSQAMAALQiKTTNGCSQAMAALQNiTTN

GCSQAMAALQNLiTNGCSQAMAALQNLPiNGCSQAMAALQNLPQiGCSQAMAALQN

LPQCiCSQAMAALQNLPQCSiSQAMAALQNLPQCSPiQAMAALQNLPQCSPDiAMAA

LQNLPQCSPDEiMAALQNLPQCSPDEIiAALQNLPQCSPDEIMiALQNLPQCSPDEIM

SiLQNLPQCSPDEIMSYiQNLPQCSPDEIMSYAiNLPQCSPDEIMSYAQiLPQCSPDEI

MSYAQKiPQCSPDEIMSYAQKIiQCSPDEIMSYAQKIFiCSPDEIMSYAQKIFKiSPDEI

MSYAQKIFKIiPDEIMSYAQKIFKILiDEIMSYAQKIFKILDiEIMSYAQKIFKILDEiIMSYA

QKIFKILDEEiMSYAQKIFKILDEERiSYAQKIFKILDEERDiYAQKIFKILDEERDKiAQKIF

KILDEERDKViQKIFKILDEERDKVLiKIFKILDEERDKVLTiIFKILDEERDKVLTHiFKILD

EERDKVLTHIiKILDEERDKVLTHIDiILDEERDKVLTHIDHiLDEERDKVLTHIDHIiDEER

DKVLTHIDHIFiEERDKVLTHIDHIFMiERDKVLTHIDHIFMDiRDKVLTHIDHIFMDIiDKV

LTHIDHIFMDILiKVLTHIDHIFMDILTiVLTHIDHIFMDILTTiLTHIDH IFMDILTTCiTHIDHI

FMDILTTCViHIDHIFMDILTTCVEiIDHIFMDILTTCVETiDHIFMDILTTCVETMiHIFMDIL

TTCVETMCilFMDILTTCVETMCNiFMDILTTCVETMCNEiMDILTTCVETMCNEYjDILT TCVETMCNEYKilLTTCVETMCNEYKVjLTTCVETMCNEYKVTjTTCVETMCNEYKVT

SiTCVETMCNEYKVTSDiCVETMCNEYKVTSDAjVETMCNEYKVTSDACjETMCNEYK

VTSDACM;TMCNEYKVTSDACMM;MCNEYKVTSDACMMT;CNEYKVTSDACMMTM;

NEYKVTSDACMMTMYiEYKVTSDACMMTMYGiYKVTSDACMMTMYGGiKVTSDAC

MMTMYGGljVTSDACMMTMYGGlSjTSDACMMTMYGGlSLjSDACMMTMYGGlSLL;

DACMMTMYGGISLLSiACMMTMYGGISLLSEjCMMTMYGGISLLSEFjMMTMYGGISL

LSEFCiMTMYGGISLLSEFCRiTMYGGISLLSEFCRVjMYGGISLLSEFCRVLjYGGISLL

SEFCRVLCiGGISLLSEFCRVLCCiGISLLSEFCRVLCCYiISLLSEFCRVLCCYViSLLS

EFCRVLCCYVLjLLSEFCRVLCCYVLEjLSEFCRVLCCYVLEEiSEFCRVLCCYVLEET;

EFCRVLCCYVLEETS;FCRVLCCYVLEETSV;CRVLCCYVLEETSVM;RVLCCYVLEET

SVMLjVLCCYVLEETSVMLAjLCCYVLEETSVMLAKiCCYVLEETSVMLAKRiCYVLEE

TSVMLAKRPiYVLEETSVMLAKRPLjVLEETSVMLAKRPLIjLEETSVMLAKRPLITjEET

SVMLAKRPLITKjETSVMLAKRPLITKPjTSVMLAKRPLITKPEiSVMLAKRPLITKPEVjV

MLAKRPLITKPEVIjMLAKRPLITKPEVISiLAKRPLITKPEVISVjAKRPLITKPEVISVMjK

RPLITKPEVISVMKiRPLITKPEVISVMKRiPLITKPEVISVMKRRiUTKPEVISVMKRRI;!

TKPEVISVM KRRIEjTKPEVISVMKRRIEEjKPEVISVMKRRIEEIjPEVISVM KRRIEEIC;

EVISVM KRRIEEICMiVISVMKRRIEEICMKJSVMKRRIEEICMKViSVMKRRIEEICMKV

F;VM KRRIEEICMKVFAiMKRRIEEICMKVFAQiKRRIEEICMKVFAQYjRRIEEICMKVF

AQYIiRIEEICMKVFAQYILiIEEICMKVFAQYILGiEEICMKVFAQYILGAiEICMKVFAQYI

LGADiICMKVFAQYILGADPiCMKVFAQYILGADPLiMKVFAQYILGADPLRiKVFAQYIL

GADPLRViVFAQYILGADPLRVCiFAQYILGADPLRVCS ;AQYILGADPLRVCSP;QYIL

GADPLRVCSPSiYILGADPLRVCSPSViILGADPLRVCSPSVDiLGADPLRVCSPSVDD

;GADPLRVCSPSVDDL;ADPLRVCSPSVDDLR;DPLRVCSPSVDDLRA;PLRVCSPSV

DDLRAIiLRVCSPSVDDLRAIAiRVCSPSVDDLRAIAEiVCSPSVDDLRAIAEEiCSPSVD

DLRAIAEESiSPSVDDLRAIAEESDiPSVDDLRAIAEESDEiSVDDLRAIAEESDEEiVDD

LRAIAEESDEEEiDDLRAIAEESDEEEAiDLRAIAEESDEEEAIiLRAIAEESDEEEAIViR

AIAEESDEEEAIVAiAIAEESDEEEAIVAYiIAEESDEEEAIVAYTiAEESDEEEAIVAYTL;

EESDEEEAIVAYTLAiESDEEEAIVAYTLATiSDEEEAIVAYTLATAiDEEEAIVAYTLATA

GiEEEAIVAYTLATAGAiEEAIVAYTLATAGASiEAIVAYTLATAGASSiAIVAYTLATAGA

SSSiIVAYTLATAGASSSDiVAYTLATAGASSSDSiAYTLATAGASSSDSLiYTLATAGA

SSSDSLViTLATAGASSSDSLVSiLATAGASSSDSLVSPiATAGASSSDSLVSPPiTAG

ASSSDSLVSPPEiAGASSSDSLVSPPESiGASSSDSLVSPPESPiASSSDSLVSPPES

PViSSSDSLVSPPESPVPiSSDSLVSPPESPVPAiSDSLVSPPESPVPATiDSLVSPPE

SPVPATIiSLVSPPESPVPATIPiLVSPPESPVPATIPLiVSPPESPVPATIPLSiSPPESP

VPATIPLSSiPPESPVPATIPLSSViPESPVPATIPLSSVIiESPVPATIPLSSVIViSPVPAT

IPLSSVIVAiPVPATIPLSSVIVAEiVPATIPLSSVIVAENiPATIPLSSVIVAENSiATIPLSS

VIVAENSDiTIPLSSVIVAENSDQiIPLSSVIVAENSDQEiPLSSVIVAENSDQEEiLSSVIV

AENSDQEESiSSVIVAENSDQEESEiSVIVAENSDQEESEQiVIVAENSDQEESEQSiIV

AENSDQEESEQSDiVAENSDQEESEQSDEiAENSDQEESEQSDEEiENSDQEESEQ

SDEEQ;NSDQEESEQSDEEQE;SDQEESEQSDEEQEE;DQEESEQSDEEQEEG;QE

ESEQSDEEQEEGA;EESEQSDEEQEEGAQ;ESEQSDEEQEEGAQE;SEQSDEEQEE

GAQEE;EQSDEEQEEGAQEER;QSDEEQEEGAQEERE;SDEEQEEGAQEERED;DE

EQEEGAQEEREDT;EEQEEGAQEEREDTV;EQEEGAQEEREDTVS;QEEGAQEERE

DTVSViEEGAQEEREDTVSVKiEGAQEEREDTVSVKSiGAQEEREDTVSVKSEiAQEE

REDTVSVKSEPiQEEREDTVSVKSEPViEEREDTVSVKSEPVSiEREDTVSVKSEPVS

EiREDTVSVKSEPVSEIiEDTVSVKSEPVSEIEiDTVSVKSEPVSEIEEiTVSVKSEPVSE

IEEViVSVKSEPVSEIEEVAiSVKSEPVSEIEEVASiVKSEPVSEIEEVASEiKSEPVSEIE

EVASEEiSEPVSEIEEVASEEEiEPVSEIEEVASEEEEiPVSEIEEVASEEEEDiVSEIEE

VASEEEEDGiSEIEEVASEEEEDGAiEIEEVASEEEEDGAEiIEEVASEEEEDGAEEiEE

VASEEEEDGAEEPiEVASEEEEDGAEEPTiVASEEEEDGAEEPTAiASEEEEDGAEEP

TAS;SEEEEDGAEEPTASG;EEEEDGAEEPTASGG;EEEDGAEEPTASGGK;EEDGAE

EPTASGGKSiEDGAEEPTASGGKSTiDGAEEPTASGGKSTHiGAEEPTASGGKSTHP

;AEEPTASGGKSTHPM;EEPTASGGKSTHPMV;EPTASGGKSTHPMVT;PTASGGKS

THPMVTRiTASGGKSTHPMVTRSiASGGKSTHPMVTRSKiSGGKSTHPMVTRSKAiG

GKSTHPMVTRSKADiGKSTHPMVTRSKADQiKSTHPMVTRSKADQLiSTHPMVTRSK

ADQLPiTHPMVTRSKADQLPGiHPMVTRSKADQLPGPiPMVTRSKADQLPGPCiMVT

RSKADQLPGPCIiVTRSKADQLPG PCIAiTRSKADQLPG PCIASiRSKADQLPGPCIAS

TjSKADQLPGPCIASTPiKADQLPGPCIASTPKiADQLPGPCIASTPKKjDQLPGPCIAS TPKKH;QLPGPCIASTPKKHR;LPG PCIASTPKKHRG;

16 mers:

MESSAKRKMDPDNPDEjESSAKRKMDPDNPDEGjSSAKRKMDPDNPDEGPjSAKRK

MDPDNPDEGPSjAKRKMDPDNPDEGPSSjKRKMDPDNPDEGPSSKjRKMDPDNPD

EGPSSKVjKMDPDNPDEGPSSKVPjMDPDNPDEGPSSKVPRjDPDNPDEGPSSKVP

RPiPDNPDEGPSSKVPRPEjDNPDEGPSSKVPRPETjNPDEGPSSKVPRPETPjPDE

GPSSKVPRPETPVjDEGPSSKVPRPETPVTjEGPSSKVPRPETPVTKjGPSSKVPRPE

TPVTKAjPSSKVPRPETPVTKATiSSKVPRPETPVTKATTjSKVPRPETPVTKATTFjKV

PRPETPVTKATTFL;VPRPETPVTKATTFLQ;PRPETPVTKATTFLQT;RPETPVTKATT

FLQTM;PETPVTKATTFLQTML;ETPVTKATTFLQTMLR;TPVTKATTFLQTMLRK;PVT

KATTFLQTMLRKE;VTKATTFLQTMLRKEV;TKATTFLQTMLRKEVN;KATTFLQTMLR

KEVNSjATTFLQTMLRKEVNSQiTTFLQTMLRKEVNSQLjTFLQTMLRKEVNSQLSjFL

QTMLRKEVNSQLSL;LQTMLRKEVNSQLSLG;QTMLRKEVNSQLSLGD;TMLRKEVNS

QLSLGDPjMLRKEVNSQLSLGDPLjLRKEVNSQLSLGDPLFjRKEVNSQLSLGDPLFP;

KEVNSQLSLGDPLFPEjEVNSQLSLGDPLFPELjVNSQLSLGDPLFPELAjNSQLSLGD

PLFPELAEiSQLSLGDPLFPELAEEjQLSLGDPLFPELAEESjLSLGDPLFPELAEESLjS

LGDPLFPELAEESLKjLGDPLFPELAEESLKTjGDPLFPELAEESLKTFjDPLFPELAEE

SLKTFEjPLFPELAEESLKTFEQiLFPELAEESLKTFEQVjFPELAEESLKTFEQVTjPEL

AEESLKTFEQVTEjELAEESLKTFEQVTEDjLAEESLKTFEQVTEDCjAEESLKTFEQVT

EDCNiEESLKTFEQVTEDCNEjESLKTFEQVTEDCNENiSLKTFEQVTEDCNENPjLKT

FEQVTEDCNENPEiKTFEQVTEDCNENPEKiTFEQVTEDCNENPEKDjFEQVTEDCN

ENPEKDVjEQVTEDCNENPEKDVLiQVTEDCNENPEKDVLTjVTEDCNENPEKDVLTE jTEDCNENPEKDVLTELiEDCNENPEKDVLTELVjDCNENPEKDVLTELVKiCNENPEK

DVLTELVKQiNENPEKDVLTELVKQIiENPEKDVLTELVKQIKiNPEKD VLTELVKQIKV;

PEKDVLTELVKQIKVRiEKDVLTELVKQIKVRViKDVLTELVKQIKVRVDiDVLTELVKQI

KVRVDMiVLTELVKQIKVRVDMViLTELVKQIKVRVDMVRiTELVKQIKVRVDMVRH iEL

VKQIKVRVDMVRHRiLVKQIKVRVDMVRHRIiVKQIKVRVDMVRHRIKiKQIKVRVDMV

RHRIKEiQIKVRVDMVRHRIKEHiIKVRVDMVRHRIKEHMiKVRVDMVRHRIKEHMLiV

RVDMVRHRIKEHMLKiRVDMVRHRIKEHMLKKiVDMVRHRIKEHMLKKYiDMVRHRIK

EHMLKKYT;MVRHRIKEHMLKKYTQ;VRHRIKEHMLKKYTQT;RHRIKEHMLKKYTQTE

;HRIKEHMLKKYTQTEE;RIKEHMLKKYTQTEEK;IKEHMLKKYTQTEEKF;KEHMLKKY

TQTEEKFT;EHMLKKYTQTEEKFTG;HMLKKYTQTEEKFTGA;MLKKYTQTEEKFTGA

F;LKKYTQTEEKFTGAFN;KKYTQTEEKFTGAFNM;KYTQTEEKFTGAFNMM;YTQTEE

KFTGAFNMMG;TQTEEKFTGAFNMMGG;QTEEKFTGAFNMMGGC;TEEKFTGAFNM

MGGCLiEEKFTGAFNMMGGCLQiEKFTGAFNMMGGCLQNiKFTGAFNMMGGCLQN

AiFTGAFNMMGGCLQNALiTGAFNMMGGCLQNALDiGAFNMMGGCLQNALDIiAFN

MMGGCLQNALDILiFNMMGGCLQNALDILDiNMMGGCLQNALDILDKiMMGGCLQNA

LDILDKViMGGCLQNALDILDKVHiGGCLQNALDILDKVHEiGCLQNALDILDKVHEPiC

LQNALDILDKVHEPFiLQNALDILDKVHEPFEiQNALDILDKVHEPFEDiNALDILDKVHE

PFEDMiALDILDKVHEPFEDMKiLDILDKVHEPFEDMKCiDILDKVHEPFEDMKCIiILDK

VHEPFEDMKCIGiLDKVHEPFEDMKCIGLiDKVHEPFEDMKCIGLTiKVHEPFEDMKCI

GLTMiVHEPFEDMKCIGLTMQiHEPFEDMKCIGLTMQSiEPFEDMKCIGLTMQSMiPF

EDMKCIGLTMQSMYiFEDMKCIGLTMQSMYEiEDMKCIGLTMQSMYENiDMKCIGLT

MQSMYENYiMKCIGLTMQSMYENYIiKCIGLTMQSMYENYIViCIGLTMQSMYENYIVP

;IGLTMQSMYENYIVPE;GLTMQSMYENYIVPED;LTMQSMYENYIVPEDK;TMQSMYE

NYIVPEDKRiMQSMYENYIVPEDKREiQSMYENYIVPEDKREMiSMYENYIVPEDKRE

MWiMYENYIVPEDKREMWMiYENYIVPEDKREMWMAiENYIVPEDKREMWMACiNYI

VPEDKREMWMACIiYIVPEDKREMWMACIKiIVPEDKREMWMACIKEiVPEDKREMW

MACIKELiPEDKREMWMACIKELHiEDKREMWMACIKELHDiDKREMWMACIKELHD

ViKREMWMACIKELHDVSiREMWMACIKELHDVSKiEMWMACIKELHDVSKGiMWMA

CIKELHDVSKGAiWMACIKELHDVSKGAAiMACIKELHDVSKGAAN iACIKELHDVSKG

AANKiCIKELHDVSKGAANKLiIKELHDVSKGAANKLGiKELHDVSKGAANKLGGiELH

DVSKGAAN KLGGAiLHDVSKGAAN KLGGALiHDVSKGAAN KLGGALQ;DVSKGAANK

LGGALQA;VSKGAANKLGGALQAK;SKGAANKLGGALQAKA;KGAANKLGGALQAKA

RiGAAN KLGGALQAKARA;AANKLGGALQAKARAK;ANKLGGALQAKARAKK;NKLGG

ALQAKARAKKD;KLGGALQAKARAKKDE;LGGALQAKARAKKDEL;GGALQAKARAK

KDELR;GALQAKARAKKDELRR;ALQAKARAKKDELRRK;LQAKARAKKDELRRKM ;Q AKARAKKDELRRKMM;AKARAKKDELRRKMMY;KARAKKDELRRKMMYM;ARAKKD

ELRRKMMYMCjRAKKDELRRKMMYMCYiAKKDELRRKMMYMCYRjKKDELRRKMM

YMCYRNjKDELRRKMMYMCYRNIjDELRRKMMYMCYRNIEjELRRKMMYMCYRNIEF

;LRRKMMYMCYRN IEFFiRRKMMYMCYRNIEFFTiRKMMYMCYRNIEFFTKiKMMYM

CYRNIEFFTKNjMMYMCYRNIEFFTKNSjMYMCYRNIEFFTKNSAjYMCYRNIEFFTKN

SAFjMCYRNIEFFTKNSAFPiCYRNIEFFTKNSAFPKjYRNIEFFTKNSAFPKTjRNIEFF

TKNSAFPKTTjNIEFFTKNSAFPKTTNjlEFFTKNSAFPKTTNGjEFFTKNSAFPKTTNG

CiFFTKNSAFPKTTNGCSiFTKNSAFPKTTNGCSQiTKNSAFPKTTNGCSQAjKNSAFP

KTTNGCSQAMiNSAFPKTTNGCSQAMAiSAFPKTTNGCSQAMAAiAFPKTTNGCSQA

MAALiFPKTTNGCSQAMAALQiPKTTNGCSQAMAALQNiKTTNGCSQAMAALQNLiT

TNGCSQAMAALQNLPiTNGCSQAMAALQNLPQiNGCSQAMAALQNLPQCiGCSQAM

AALQNLPQCSiCSQAMAALQNLPQCSPiSQAMAALQNLPQCSPDiQAMAALQNLPQ

CSPDEiAMAALQNLPQCSPDEIiMAALQNLPQCSPDEIMiAALQNLPQCSPDEIMSiAL

QNLPQCSPDEIMSYiLQNLPQCSPDEIMSYAiQNLPQCSPDEIMSYAQiNLPQCSPDEI

MSYAQKiLPQCSPDEIMSYAQKIiPQCSPDEIMSYAQKIFiQCSPDEIMSYAQKIFKiCS

PDEIMSYAQKIFKIiSPDEIMSYAQKIFKILiPDEIMSYAQKIFKILDiDEIMSYAQKIFKILD

EiEIMSYAQKIFKILDEEiIMSYAQKIFKILDEERiMSYAQKIFKILDEERDiSYAQKIFKILD

EERDKiYAQKIFKILDEERDKViAQKIFKILDEERDKVLiQKIFKILDEERDKVLTiKIFKILD

EERDKVLTHiIFKILDEERDKVLTHIiFKILDEERDKVLTHIDiKILDEERDKVLTHIDHiILD

EERDKVLTHIDHIiLDEERDKVLTHIDHIFiDEERDKVLTHIDHIFMiEERDKVLTHIDHIF

MDiERDKVLTHIDHIFMDIiRDKVLTHIDHIFMDILiDKVLTHIDHIFMDILTiKVLTHIDHIF

MDILTTiVLTHIDHIFMDILTTCiLTHIDHIFMDILTTCViTHIDHIFMDILTTCVEiHIDHIFM

DILTTCVETiIDHIFMDILTTCVETMiDHIFMDILTTCVETMCiHIFMDILTTCVETMCNiIF

MDILTTCVETMCNEiFMDILTTCVETMCNEYiMDILTTCVETMCNEYKiDILTTCVETMC

NEYKViILTTCVETMCNEYKVTiLTTCVETMCNEYKVTSiTTCVETMCNEYKVTSDiTC

VETMCNEYKVTSDA;CVETMCNEYKVTSDAC;VETMCNEYKVTSDACM;ETMCNEYK

VTSDACMM;TMCNEYKVTSDACMMT;MCNEYKVTSDACMMTM;CNEYKVTSDACM

MTMYiNEYKVTSDACMMTMYGiEYKVTSDACMMTMYGGiYKVTSDACMMTMYGGI;

KVTSDACMMTMYGGISiVTSDACMMTMYGGISLiTSDACMMTMYGGISLLiSDACMM

TMYGGISLLSiDACMMTMYGGISLLSEiACMMTMYGGISLLSEFiCMMTMYGGISLLS

EFCiMMTMYGGISLLSEFCRiMTMYGGISLLSEFCRViTMYGGISLLSEFCRVLiMYGG

ISLLSEFCRVLCiYGGISLLSEFCRVLCCiGGISLLSEFCRVLCCYiGISLLSEFCRVLCC

YViISLLSEFCRVLCCYVLiSLLSEFCRVLCCYVLEiLLSEFCRVLCCYVLEEiLSEFCRV

LCCYVLEETiSEFCRVLCCYVLEETSiEFCRVLCCYVLEETSViFCRVLCCYVLEETSV

MiCRVLCCYVLEETSVMLiRVLCCYVLEETSVMLAiVLCCYVLEETSVMLAKiLCCYVL

EETSVMLAKRiCCYVLEETSVMLAKRPiCYVLEETSVMLAKRPLiYVLEETSVMLAKRP

LIiVLEETSVMLAKRPLITiLEETSVMLAKRPLITKiEETSVMLAKRPLITKPiETSVMLAK

RPLITKPEiTSVMLAKRPLITKPEViSVMLAKRPLITKPEVIiVMLAKRPLITKPEVISiMLA

KRPLITKPEVISViLAKRPLITKPEVISVMiAKRPLITKPEVISVMKiKRPLITKPEVISVMK

RiRPLITKPEVISVMKRRiPLITKPEVISVMKRRIiLITKPEVISVMKRRIEiITKPEVISVMK

RRIEEiTKPEVISVMKRRIEEIiKPEVISVMKRRIEEICiPEVISVMKRRIEEICMiEVISVM

KRRIEEICMKiVISVMKRRIEEICMKViISVMKRRIEEICMKVFiSVMKRRIEEICMKVFA;

VMKRRIEEICMKVFAQiMKRRIEEICMKVFAQYiKRRIEEICMKVFAQYIiRRIEEICMKV

FAQYILiRIEEICMKVFAQYILGiIEEICMKVFAQYILGAiEEICMKVFAQYILGADiEICMK

VFAQYILGADPiICMKVFAQYILGADPLiCMKVFAQYILGADPLRiMKVFAQYILGADPL

RViKVFAQYILGADPLRVCiVFAQYILGADPLRVCSiFAQYILGADPLRVCSPiAQYILGA

DPLRVCSPSiQYILGADPLRVCSPSViYILGADPLRVCSPSVDiILGADPLRVCSPSVD

DiLGADPLRVCSPSVDDLiGADPLRVCSPSVDDLRiADPLRVCSPSVDDLRAiDPLRV

CSPSVDDLRAIiPLRVCSPSVDDLRAIAiLRVCSPSVDDLRAIAEiRVCSPSVDDLRAIA

EEiVCSPSVDDLRAIAEESiCSPSVDDLRAIAEESDiSPSVDDLRAIAEESDEiPSVDDL

RAIAEESDEEiSVDDLRAIAEESDEEEiVDDLRAIAEESDEEEAiDDLRAIAEESDEEEAI

;DLRAIAEESDEEEAIV;LRAIAEESDEEEAIVA;RAIAEESDEEEAIVAY;AIAEESDEEEA

IVAYTiIAEESDEEEAIVAYTLiAEESDEEEAIVAYTLAiEESDEEEAIVAYTLATiESDEEE

AIVAYTLATAiSDEEEAIVAYTLATAGiDEEEAIVAYTLATAGAiEEEAIVAYTLATAGAS;

EEAIVAYTLATAGASSiEAIVAYTLATAGASSSiAIVAYTLATAGASSSDiIVAYTLATAG

ASSSDSiVAYTLATAGASSSDSLiAYTLATAGASSSDSLViYTLATAGASSSDSLVSiTL

ATAGASSSDSLVSPiLATAGASSSDSLVSPPiATAGASSSDSLVSPPEiTAGASSSDSL

VSPPESiAGASSSDSLVSPPESPiGASSSDSLVSPPESPViASSSDSLVSPPESPVPiS

Preferred peptide sequences The peptide according to the present invention may be defined as outlined in the items herein below. It is to be understood that said items are not meant to be limiting to the peptide according to the present invention in that said peptide may consist of more than said 8 to 16 amino acids, but at least comprising said 8 to 16 amino acids.

Thus, in one embodiment of the present invention, the peptide may be a fragment or part of a larger protein, wherein the larger protein may be of a total length of 17, such as 18, for example 19, such as 20, for example 21 , such as 22, for example 23, such as 24, for example 25, such as 26, for example 27, such as 28, for example 29, such as 30, for example 31 , such as 32, for example 33, such as 34, for example 35, such as 36, for example 37, such as 38, for example 39, such as 40 amino acids, wherein 8 to 16 of said amino acids are defined in the items below. In another embodiment, the larger protein may be of a total length of between 20 to 30, such as 30-40, for example 40-50, such as 50-60, for example 60-70, such as 70-80, for example 80-90, such as 90-100, for example 100-150, such as 150-200, for example 200-250, such as 250- 300, for example 300-500, such as 500-1000, for example 1000-2000, such as 2000- 3000, for example 3000-4000, such as 4000-5000, for example 5000-10,000, such as 10,000-20,000, for example 20,000-30,000, such as 30,000-40,000, for example 40,000-50,000, such as 50,000-75,000, for example 75,000-100,000, such as 100,000- 250,000, for example 250,000-, 500, 000, such as 500,000-1 ,000,000 amino acids.

It is also to be understood, that the co-translational and post-translational modifications may occur either individually or in combination, on the same or different amino acid residues. Thus, in one embodiment, any one amino acid may be modified once, twice or three times with the same or different types of modifications. Furthermore, said identical and/or different modification may be present on 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17 or 18 of the amino acid residues of the peptide according to the present invention as defined in the items below. In addition, modifications may also be present on amino acid residues outside said 8 to 16 amino acids, in case the peptide is part of a larger protein.

ITEMS

1 . An antigenic peptide of between 8 to 16 consecutive amino acids, comprising at least 8 of amino acid number X₁-X₂-X₃-X₄-X₅-X₆-X₇-X₈-Xg-X₁O-XiI-X^-XiS-XM- X₁₅-X₁₆ 2. The peptide according to item 1 , wherein Xi is alanine

3. The peptide according to item 1 , wherein Xi is arginine

4. The peptide according to item 1 , wherein Xi is asparagine

5. The peptide according to item 1 , wherein Xi is aspartic acid 6. The peptide according to item 1 , wherein Xi is cysteine

7. The peptide according to item 1 , wherein Xi is glutamic acid

8. The peptide according to item 1 , wherein Xi is glutamine

9. The peptide according to item 1 , wherein X₁ is glycine

10. The peptide according to item 1 , wherein X₁ is histidine 1 1 . The peptide according to item 1 , wherein X₁ is isoleucine

12. The peptide according to item 1 , wherein X₁ is leucine

13. The peptide according to item 1 , wherein X₁ is lysine

14. The peptide according to item 1 , wherein X₁ is methionine

15. The peptide according to item 1 , wherein X₁ is phenylalanine 16. The peptide according to item 1 , wherein X₁ is proline

17. The peptide according to item 1 , wherein X₁ is serine

18. The peptide according to item 1 , wherein X₁ is threonine

19. The peptide according to item 1 , wherein X₁ is tryptophan

20. The peptide according to item 1 , wherein X₁ is tyrosine 21 . The peptide according to item 1 , wherein X₁ is valine

22. The peptide according to item 1 , wherein X₂ is alanine

23. The peptide according to item 1 , wherein X₂ is arginine

24. The peptide according to item 1 , wherein X₂ is asparagine

25. The peptide according to item 1 , wherein X₂ is aspartic acid 26. The peptide according to item 1 , wherein X₂ is cysteine

27. The peptide according to item 1 , wherein X₂ is glutamic acid

28. The peptide according to item 1 , wherein X₂ is glutamine

29. The peptide according to item 1 , wherein X₂ is glycine

30. The peptide according to item 1 , wherein X₂ is histidine 31 . The peptide according to item 1 , wherein X₂ is isoleucine

32. The peptide according to item 1 , wherein X₂ is leucine

33. The peptide according to item 1 , wherein X₂ is lysine

34. The peptide according to item 1 , wherein X₂ is methionine

35. The peptide according to item 1 , wherein X₂ is phenylalanine 36. The peptide according to item 1 , wherein X₂ is proline 37. The peptide according to item 1 , wherein X₂ is serine

38. The peptide according to item 1 , wherein X₂ is threonine

39. The peptide according to item 1 , wherein X₂ is tryptophan

40. The peptide according to item 1 , wherein X₂ is tyrosine 41 . The peptide according to item 1 , wherein X₂ is valine

42. The peptide according to item 1 , wherein X₃ is alanine

43. The peptide according to item 1 , wherein X₃ is arginine

44. The peptide according to item 1 , wherein X₃ is asparagine

45. The peptide according to item 1 , wherein X₃ is aspartic acid 46. The peptide according to item 1 , wherein X₃ is cysteine

47. The peptide according to item 1 , wherein X₃ is glutamic acid

48. The peptide according to item 1 , wherein X₃ is glutamine

49. The peptide according to item 1 , wherein X₃ is glycine

50. The peptide according to item 1 , wherein X₃ is histidine 51 . The peptide according to item 1 , wherein X₃ is isoleucine

52. The peptide according to item 1 , wherein X₃ is leucine

53. The peptide according to item 1 , wherein X₃ is lysine

54. The peptide according to item 1 , wherein X₃ is methionine

55. The peptide according to item 1 , wherein X₃ is phenylalanine 56. The peptide according to item 1 , wherein X₃ is proline

57. The peptide according to item 1 , wherein X₃ is serine

58. The peptide according to item 1 , wherein X₃ is threonine

59. The peptide according to item 1 , wherein X₃ is tryptophan

60. The peptide according to item 1 , wherein X₃ is tyrosine 61 . The peptide according to item 1 , wherein X₃ is valine

62. The peptide according to item 1 , wherein X₄ is alanine

63. The peptide according to item 1 , wherein X₄ is arginine

64. The peptide according to item 1 , wherein X₄ is asparagine

65. The peptide according to item 1 , wherein X₄ is aspartic acid 66. The peptide according to item 1 , wherein X₄ is cysteine

67. The peptide according to item 1 , wherein X₄ is glutamic acid

68. The peptide according to item 1 , wherein X₄ is glutamine

69. The peptide according to item 1 , wherein X₄ is glycine

70. The peptide according to item 1 , wherein X₄ is histidine 71 . The peptide according to item 1 , wherein X₄ is isoleucine 72. The peptide according to item 1 , wherein X₄ is leucine

73. The peptide according to item 1 , wherein X₄ is lysine

74. The peptide according to item 1 , wherein X₄ is methionine

75. The peptide according to item 1 , wherein X₄ is phenylalanine 76. The peptide according to item 1 , wherein X₄ is proline

77. The peptide according to item 1 , wherein X₄ is serine

78. The peptide according to item 1 , wherein X₄ is threonine

79. The peptide according to item 1 , wherein X₄ is tryptophan

80. The peptide according to item 1 , wherein X₄ is tyrosine 81 . The peptide according to item 1 , wherein X₄ is valine

82. The peptide according to item 1 , wherein X₅ is alanine

83. The peptide according to item 1 , wherein X₅ is arginine

84. The peptide according to item 1 , wherein X₅ is asparagine

85. The peptide according to item 1 , wherein X₅ is aspartic acid 86. The peptide according to item 1 , wherein X₅ is cysteine

87. The peptide according to item 1 , wherein X₅ is glutamic acid

88. The peptide according to item 1 , wherein X₅ is glutamine

89. The peptide according to item 1 , wherein X₅ is glycine

90. The peptide according to item 1 , wherein X₅ is histidine 91 . The peptide according to item 1 , wherein X₅ is isoleucine

92. The peptide according to item 1 , wherein X₅ is leucine

93. The peptide according to item 1 , wherein X₅ is lysine

94. The peptide according to item 1 , wherein X₅ is methionine

95. The peptide according to item 1 , wherein X₅ is phenylalanine 96. The peptide according to item 1 , wherein X₅ is proline

97. The peptide according to item 1 , wherein X₅ is serine

98. The peptide according to item 1 , wherein X₅ is threonine

99. The peptide according to item 1 , wherein X₅ is tryptophan

100. The peptide according to item 1 , wherein X₅ is tyrosine 101 . The peptide according to item 1 , wherein X₅ is valine

102. The peptide according to item 1 , wherein X₆ is alanine

103. The peptide according to item 1 , wherein X₆ is arginine

104. The peptide according to item 1 , wherein X₆ is asparagine

105. The peptide according to item 1 , wherein X₆ is aspartic acid 106. The peptide according to item 1 , wherein X₆ is cysteine 107. The peptide according to item 1 , wherein X₆ is glutamic acid

108. The peptide according to item 1 , wherein X₆ is glutamine

109. The peptide according to item 1 , wherein X₆ is glycine

1 10. The peptide according to item 1 , wherein X₆ is histidine 1 1 1 . The peptide according to item 1 , wherein X₆ is isoleucine

1 12. The peptide according to item 1 , wherein X₆ is leucine

1 13. The peptide according to item 1 , wherein X₆ is lysine

1 14. The peptide according to item 1 , wherein X₆ is methionine

1 15. The peptide according to item 1 , wherein X₆ is phenylalanine 1 16. The peptide according to item 1 , wherein X₆ is proline

1 17. The peptide according to item 1 , wherein X₆ is serine

1 18. The peptide according to item 1 , wherein X₆ is threonine

1 19. The peptide according to item 1 , wherein X₆ is tryptophan

120. The peptide according to item 1 , wherein X₆ is tyrosine 121 . The peptide according to item 1 , wherein X₆ is valine

122. The peptide according to item 1 , wherein X₇ is alanine

123. The peptide according to item 1 , wherein X₇ is arginine

124. The peptide according to item 1 , wherein X₇ is asparagine

125. The peptide according to item 1 , wherein X₇ is aspartic acid 126. The peptide according to item 1 , wherein X₇ is cysteine

127. The peptide according to item 1 , wherein X₇ is glutamic acid

128. The peptide according to item 1 , wherein X₇ is glutamine

129. The peptide according to item 1 , wherein X₇ is glycine

130. The peptide according to item 1 , wherein X₇ is histidine 131 . The peptide according to item 1 , wherein X₇ is isoleucine

132. The peptide according to item 1 , wherein X₇ is leucine

133. The peptide according to item 1 , wherein X₇ is lysine

134. The peptide according to item 1 , wherein X₇ is methionine

135. The peptide according to item 1 , wherein X₇ is phenylalanine 136. The peptide according to item 1 , wherein X₇ is proline

137. The peptide according to item 1 , wherein X₇ is serine

138. The peptide according to item 1 , wherein X₇ is threonine

139. The peptide according to item 1 , wherein X₇ is tryptophan

140. The peptide according to item 1 , wherein X₇ is tyrosine 141 . The peptide according to item 1 , wherein X₇ is valine 142. The peptide according to item 1 , wherein X₈ is alanine

143. The peptide according to item 1 , wherein X₈ is arginine

144. The peptide according to item 1 , wherein X₈ is asparagine

145. The peptide according to item 1 , wherein X₈ is aspartic acid 146. The peptide according to item 1 , wherein X₈ is cysteine

147. The peptide according to item 1 , wherein X₈ is glutamic acid

148. The peptide according to item 1 , wherein X₈ is glutamine

149. The peptide according to item 1 , wherein X₈ is glycine

150. The peptide according to item 1 , wherein X₈ is an histidine 151 . The peptide according to item 1 , wherein X₈ is isoleucine

152. The peptide according to item 1 , wherein X₈ is leucine

153. The peptide according to item 1 , wherein X₈ is lysine

154. The peptide according to item 1 , wherein X₈ is methionine

155. The peptide according to item 1 , wherein X₈ is phenylalanine 156. The peptide according to item 1 , wherein X₈ is proline

157. The peptide according to item 1 , wherein X₈ is serine

158. The peptide according to item 1 , wherein X₈ is threonine

159. The peptide according to item 1 , wherein X₈ is tryptophan

160. The peptide according to item 1 , wherein X₈ is tyrosine 161 . The peptide according to item 1 , wherein X₈ is valine

162. The peptide according to item 1 , wherein X₉ is alanine

163. The peptide according to item 1 , wherein X₉ is arginine

164. The peptide according to item 1 , wherein X₉ is asparagine

165. The peptide according to item 1 , wherein X₉ is aspartic acid 166. The peptide according to item 1 , wherein X₉ is cysteine

167. The peptide according to item 1 , wherein X₉ is glutamic acid

168. The peptide according to item 1 , wherein X₉ is glutamine

169. The peptide according to item 1 , wherein X₉ is glycine

170. The peptide according to item 1 , wherein X₉ is an histidine 171 . The peptide according to item 1 , wherein X₉ is isoleucine

172. The peptide according to item 1 , wherein X₉ is leucine

173. The peptide according to item 1 , wherein X₉ is lysine

174. The peptide according to item 1 , wherein X₉ is methionine

175. The peptide according to item 1 , wherein X₉ is phenylalanine 176. The peptide according to item 1 , wherein X₉ is proline 177. The peptide according to item 1 , wherein X₉ is serine

178. The peptide according to item 1 , wherein X₉ is threonine

179. The peptide according to item 1 , wherein X₉ is tryptophan

180. The peptide according to item 1 , wherein X₉ is tyrosine 181 . The peptide according to item 1 , wherein X₉ is valine

182. The peptide according to item 1 , wherein X₉ is alanine

183. The peptide according to item 1 , wherein X₉ is arginine

184. The peptide according to item 1 , wherein X₉ is asparagine

185. The peptide according to item 1 , wherein X₉ is aspartic acid 186. The peptide according to item 1 , wherein X₉ is cysteine

187. The peptide according to item 1 , wherein X₉ is glutamic acid

188. The peptide according to item 1 , wherein X₉ is glutamine

189. The peptide according to item 1 , wherein X₉ is glycine

190. The peptide according to item 1 , wherein X₉ is an histidine 191 . The peptide according to item 1 , wherein X₉ is isoleucine

192. The peptide according to item 1 , wherein X₉ is leucine

193. The peptide according to item 1 , wherein X₉ is lysine

194. The peptide according to item 1 , wherein X₉ is methionine

195. The peptide according to item 1 , wherein X₉ is phenylalanine 196. The peptide according to item 1 , wherein X₉ is proline

197. The peptide according to item 1 , wherein X₉ is serine

198. The peptide according to item 1 , wherein X₉ is threonine

199. The peptide according to item 1 , wherein X₉ is tryptophan

200. The peptide according to item 1 , wherein X₉ is tyrosine 201 . The peptide according to item 1 , wherein X₉ is valine

202. The peptide according to item 1 , wherein X₁₀ is alanine

203. The peptide according to item 1 , wherein X₁₀ is arginine

204. The peptide according to item 1 , wherein X₁₀ is asparagine

205. The peptide according to item 1 , wherein X₁₀ is aspartic acid 206. The peptide according to item 1 , wherein X₁₀ is cysteine

207. The peptide according to item 1 , wherein X₁₀ is glutamic acid

208. The peptide according to item 1 , wherein X₁₀ is glutamine

209. The peptide according to item 1 , wherein X₁₀ is glycine

210. The peptide according to item 1 , wherein X₁₀ is an histidine 21 1 . The peptide according to item 1 , wherein Xi₀ is isoleucine 212. The peptide according to item 1 , wherein Xi₀ is leucine

213. The peptide according to item 1 , wherein Xi₀ is lysine

214. The peptide according to item 1 , wherein Xi₀ is methionine

215. The peptide according to item 1 , wherein Xi₀ is phenylalanine 216. The peptide according to item 1 , wherein Xi₀ is proline

217. The peptide according to item 1 , wherein Xi₀ is serine

218. The peptide according to item 1 , wherein Xi₀ is threonine

219. The peptide according to item 1 , wherein X₁₀ is tryptophan

220. The peptide according to item 1 , wherein X₁₀ is tyrosine 221 . The peptide according to item 1 , wherein X₁₀ is valine

222. The peptide according to item 1 , wherein X₁₁ is alanine

223. The peptide according to item 1 , wherein X₁₁ is arginine

224. The peptide according to item 1 , wherein X₁₁ is asparagine

225. The peptide according to item 1 , wherein X₁₁ is aspartic acid 226. The peptide according to item 1 , wherein X₁₁ is cysteine

227. The peptide according to item 1 , wherein X₁₁ is glutamic acid

228. The peptide according to item 1 , wherein X₁₁ is glutamine

229. The peptide according to item 1 , wherein X₁₁ is glycine

230. The peptide according to item 1 , wherein X₁₁ is an histidine 231 . The peptide according to item 1 , wherein X₁₁ is isoleucine

232. The peptide according to item 1 , wherein X₁₁ is leucine

233. The peptide according to item 1 , wherein X₁₁ is lysine

234. The peptide according to item 1 , wherein X₁₁ is methionine

235. The peptide according to item 1 , wherein X₁₁ is phenylalanine 236. The peptide according to item 1 , wherein X₁₁ is proline

237. The peptide according to item 1 , wherein X₁₁ is serine

238. The peptide according to item 1 , wherein X₁₁ is threonine

239. The peptide according to item 1 , wherein X₁₁ is tryptophan

240. The peptide according to item 1 , wherein X₁₁ is tyrosine 241 . The peptide according to item 1 , wherein X₁₁ is valine

242. The peptide according to item 1 , wherein X₁₂ is alanine

243. The peptide according to item 1 , wherein X₁₂ is arginine

244. The peptide according to item 1 , wherein X₁₂ is asparagine

245. The peptide according to item 1 , wherein X₁₂ is aspartic acid 246. The peptide according to item 1 , wherein X₁₂ is cysteine 247. The peptide according to item 1 , wherein Xi₂ is glutamic acid

248. The peptide according to item 1 , wherein Xi₂ is glutamine

249. The peptide according to item 1 , wherein Xi₂ is glycine

250. The peptide according to item 1 , wherein Xi₂ is histidine

251 . The peptide according to item 1 , wherein Xi₂ is isoleucine

252. The peptide according to item 1 , wherein Xi₂ is leucine

253. The peptide according to item 1 , wherein Xi₂ is lysine

254. The peptide according to item 1 , wherein Xi₂ is methionine

255. The peptide according to item 1 , wherein Xi₂ is phenylalanine

256. The peptide according to item 1 , wherein Xi₂ is proline

257. The peptide according to item 1 , wherein Xi₂ is serine

258. The peptide according to item 1 , wherein Xi₂ is threonine

259. The peptide according to item 1 , wherein Xi₂ is tryptophan

260. The peptide according to item 1 , wherein Xi₂ is tyrosine

261 . The peptide according to item 1 , wherein Xi₂ is valine

262. The peptide according to item 1 , wherein Xi₃ is alanine

263. The peptide according to item 1 , wherein Xi₃ is arginine

264. The peptide according to item 1 , wherein Xi₃ is asparagine

265. The peptide according to item 1 , wherein Xi₃ is aspartic acid

266. The peptide according to item 1 , wherein Xi₃ is cysteine

267. The peptide according to item 1 , wherein Xi₃ is glutamic acid

268. The peptide according to item 1 , wherein Xi₃ is glutamine

269. The peptide according to item 1 , wherein Xi₃ is glycine

270. The peptide according to item 1 , wherein Xi₃ is histidine

271 . The peptide according to item 1 , wherein Xi₃ is isoleucine

272. The peptide according to item 1 , wherein Xi₃ is leucine

273. The peptide according to item 1 , wherein Xi₃ is lysine

274. The peptide according to item 1 , wherein Xi₃ is methionine

275. The peptide according to item 1 , wherein Xi₃ is phenylalanine

276. The peptide according to item 1 , wherein Xi₃ is proline

277. The peptide according to item 1 , wherein Xi₃ is serine

278. The peptide according to item 1 , wherein Xi₃ is threonine

279. The peptide according to item 1 , wherein Xi₃ is tryptophan

280. The peptide according to item 1 , wherein Xi₃ is tyrosine

281 . The peptide according to item 1 , wherein Xi₃ is valine -.

282. The peptide according to item 1 , wherein X₁₄ is alanine

283. The peptide according to item 1 , wherein X₁₄ is arginine

284. The peptide according to item 1 , wherein X₁₄ is asparagine

285. The peptide according to item 1 , wherein X₁₄ is aspartic acid

286. The peptide according to item 1 , wherein X₁₄ is cysteine

287. The peptide according to item 1 , wherein X₁₄ is glutamic acid

288. The peptide according to item 1 , wherein X₁₄ is glutamine

289. The peptide according to item 1 , wherein X₁₄ is glycine

290. The peptide according to item 1 , wherein X₁₄ is histidine

291 . The peptide according to item 1 , wherein X₁₄ is isoleucine

292. The peptide according to item 1 , wherein X₁₄ is leucine

293. The peptide according to item 1 , wherein X₁₄ is lysine

294. The peptide according to item 1 , wherein X₁₄ is methionine

295. The peptide according to item 1 , wherein X₁₄ is phenylalanine

296. The peptide according to item 1 , wherein X₁₄ is proline

297. The peptide according to item 1 , wherein X₁₄ is serine

298. The peptide according to item 1 , wherein X₁₄ is threonine

299. The peptide according to item 1 , wherein X₁₄ is tryptophan

300. The peptide according to item 1 , wherein X₁₄ is tyrosine

301 . The peptide according to item 1 , wherein X₁₄ is valine

302. The peptide according to item 1 , wherein X₁₅ is alanine

303. The peptide according to item 1 , wherein X₁₅ is arginine

304. The peptide according to item 1 , wherein X₁₅ is asparagine

305. The peptide according to item 1 , wherein X₁₅ is aspartic acid

306. The peptide according to item 1 , wherein X₁₅ is cysteine

307. The peptide according to item 1 , wherein X₁₅ is glutamic acid

308. The peptide according to item 1 , wherein X₁₅ is glutamine

309. The peptide according to item 1 , wherein X₁₅ is glycine

310. The peptide according to item 1 , wherein X₁₅ is histidine

31 1 . The peptide according to item 1 , wherein X₁₅ is isoleucine

312. The peptide according to item 1 , wherein X₁₅ is leucine

313. The peptide according to item 1 , wherein X₁₅ is lysine

314. The peptide according to item 1 , wherein X₁₅ is methionine

315. The peptide according to item 1 , wherein X₁₅ is phenylalanine

316. The peptide according to item 1 , wherein X₁₅ is proline 317. The peptide according to item 1 , wherein Xi₅ is serine

318. The peptide according to item 1 , wherein Xi₅ is threonine

319. The peptide according to item 1 , wherein Xi₅ is tryptophan

320. The peptide according to item 1 , wherein Xi₅ is tyrosine 321 . The peptide according to item 1 , wherein Xi₅ is valine

322. The peptide according to item 1 , wherein Xi₆ is alanine

323. The peptide according to item 1 , wherein Xi₆ is arginine

324. The peptide according to item 1 , wherein Xi₆ is asparagine

325. The peptide according to item 1 , wherein Xi₆ is aspartic acid 326. The peptide according to item 1 , wherein Xi₆ is cysteine

327. The peptide according to item 1 , wherein Xi₆ is glutamic acid

328. The peptide according to item 1 , wherein Xi₆ is glutamine

329. The peptide according to item 1 , wherein Xi₆ is glycine

330. The peptide according to item 1 , wherein Xi₆ is histidine 331 . The peptide according to item 1 , wherein Xi₆ is isoleucine

332. The peptide according to item 1 , wherein Xi₆ is leucine

333. The peptide according to item 1 , wherein Xi₆ is lysine

334. The peptide according to item 1 , wherein Xi₆ is methionine

335. The peptide according to item 1 , wherein Xi₆ is phenylalanine 336. The peptide according to item 1 , wherein Xi₆ is proline

337. The peptide according to item 1 , wherein Xi₆ is serine

338. The peptide according to item 1 , wherein Xi₆ is threonine

339. The peptide according to item 1 , wherein Xi₆ is tryptophan

340. The peptide according to item 1 , wherein Xi₆ is tyrosine 341 . The peptide according to item 1 , wherein Xi₆ is valine

342. The peptide according to any of items 2, 22, 42, 62, 82, 102, 122, 142, 162, 182, 202, 222, 242, 262, 282, 302 or 322, wherein the alanine is D-alanine

343. The peptide according to any of items 2, 22, 42, 62, 82, 102, 122, 142,

162, 182, 202, 222, 242, 262, 282, 302 or 322, wherein the alanine is L-alanine 344. The peptide according to any of items 3, 23, 43, 63, 83, 103, 123, 143,

163, 183, 203, 223, 243, 263, 283, 303 or 323, wherein the arginine is D- arginine

345. The peptide according to any of items 3, 23, 43, 63, 83, 103, 123, 143,

163, 183, 203, 223, 243, 263, 283, 303 or 323, wherein the arginine is L- arginine -. o

346. The peptide according to any of items 4, 24, 44, 64, 84, 104, 124, 144,

164, 184, 204, 224, 244, 264, 284, 304 or 324, wherein the asparagine is D- asparagine

347. The peptide according to any of items 4, 24, 44, 64, 84, 104, 124, 144, 164, 184, 204, 224, 244, 264, 284, 304 or 324, wherein the asparagine is L- asparagine

348. The peptide according to any of items 5, 25, 45, 65, 85, 105, 125, 145,

165, 185, 205, 225, 245, 265, 285, 305 or 325, wherein the aspartic acid is D- aspartic acid 349. The peptide according to any of items 5, 25, 45, 65, 85, 105, 125, 145,

165, 185, 205, 225, 245, 265, 285, 305 or 325, wherein the aspartic acid is L- aspartic acid

350. The peptide according to any of items 6, 26, 46, 66, 86, 106, 126, 146,

166, 186, 206, 226, 246, 266, 286, 306 or 326, wherein the cysteine is D- cysteine

351. The peptide according to any of items 6, 26, 46, 66, 86, 106, 126, 146,

166, 186, 206, 226, 246, 266, 286, 306 or 326, wherein the cysteine is L- cysteine

352. The peptide according to any of items 7, 27, 47, 67, 87, 107, 127, 147, 167, 187, 207, 227, 247, 267, 287, 307 or 327, wherein the glutamic acid is D- glutamic acid

353. The peptide according to any of items 7, 27, 47, 67, 87, 107, 127, 147,

167, 187, 207, 227, 247, 267, 287, 307 or 327, wherein the glutamic acid is L- glutamic acid 354. The peptide according to any of items 8, 28, 48, 68, 88, 108, 128, 148,

168, 188, 208, 228, 248, 268, 288, 308 or 328, wherein the glutamine is D- glutamine

355. The peptide according to any of items 8, 28, 48, 68, 88, 108, 128, 148,

168, 188, 208, 228, 248, 268, 288, 308 or 328, wherein the glutamine is L- glutamine

356. The peptide according to any of items 9, 29, 49, 69, 89, 109, 129, 149,

169, 189, 209, 229, 249, 269, 289, 309 or 329, wherein the glycine is D-glycine

357. The peptide according to any of items 9, 29, 49, 69, 89, 109, 129, 149, 169, 189, 209, 229, 249, 269, 289, 309 or 329, wherein the glycine is L-glycine 358. The peptide according to any of items 10, 30, 50, 70, 90, 1 10, 130, 150,

170, 190, 210, 230, 250, 270, 290, 310 or 330, wherein the histidine is D- histidine

359. The peptide according to any of items 10, 30, 50, 70, 90, 1 10, 130, 150, 170, 190, 210, 230, 250, 270, 290, 310 or 330, wherein the histidine is L- histidine

360. The peptide according to any of items 1 1 , 31 , 51 , 71 , 91 , 1 1 1 , 131 , 151 ,

171 , 191 , 21 1 , 231 , 251 , 271 , 291 , 31 1 or 331 , wherein the isoleucine is D- isoleucine 361. The peptide according to any of items 1 1 , 31 , 51 , 71 , 91 , 1 1 1 , 131 , 151 ,

171 , 191 , 21 1 , 231 , 251 , 271 , 291 , 31 1 or 331 , wherein the isoleucine is L- isoleucine

362. The peptide according to any of items 12, 32, 52, 72, 92, 1 12, 132, 152,

172, 192, 212, 232, 252, 272, 292, 312 or 332, wherein the leucine is D-leucine 363. The peptide according to any of items 12, 32, 52, 72, 92, 1 12, 132, 152,

172, 192, 212, 232, 252, 272, 292, 312 or 332, wherein the leucine is L-leucine

364. The peptide according to any of items 13, 33, 53, 73, 93, 1 13, 133, 153,

173, 193, 213, 233, 253, 273, 293, 313 or 333, wherein the lysine is D-lysine

365. The peptide according to any of items 13, 33, 53, 73, 93, 1 13, 133, 153, 173, 193, 213, 233, 253, 273, 293, 313 or 333, wherein the lysine is L-lysine

366. The peptide according to any of items 14, 34, 54, 74, 94, 1 14, 134, 154,

174, 194, 214, 234, 254, 274, 294, 314 or 334, wherein the methionine is D- methionine

367. The peptide according to any of items 14, 34, 54, 74, 94, 1 14, 134, 154, 174, 194, 214, 234, 254, 274, 294, 314 or 334, wherein the methionine is L- methionine

368. The peptide according to any of items 15, 35, 55, 75, 95, 1 15, 135, 155,

175, 195, 215, 235, 255, 275, 295, 315 or 335, wherein the phenylalanine is D- phenylalanine 369. The peptide according to any of items 15, 35, 55, 75, 95, 1 15, 135, 155,

175, 195, 215, 235, 255, 275, 295, 315 or 335, wherein the phenylalanine is L- phenylalanine

370. The peptide according to any of items 16, 36, 56, 76, 96, 1 16, 136, 156,

176, 196, 216, 236, 256, 276, 296, 316 or 336, wherein the proline is D-proline 371 . The peptide according to any of items 16, 36, 56, 76, 96, 1 16, 136, 156,

176, 196, 216, 236, 256, 276, 296, 316 or 336, wherein the proline is L-proline

372. The peptide according to any of items 17, 37, 57, 77, 97, 1 17, 137, 157,

177, 197, 217, 237, 257, 277, 297, 317 or 337, wherein the serine is D-serine 373. The peptide according to any of items 17, 37, 57, 77, 97, 1 17, 137, 157,

177, 197, 217, 237, 257, 277, 297, 317 or 337, wherein the serine is L-serine 374. The peptide according to any of items 18, 38, 58, 78, 98, 1 18, 138, 158,

178, 198, 218, 238, 258, 278, 298, 318 or 338, wherein the threonine is D- threonine 375. The peptide according to any of items 18, 38, 58, 78, 98, 1 18, 138, 158,

178, 198, 218, 238, 258, 278, 298, 318 or 338, wherein the threonine is L- threonine

376. The peptide according to any of items 19, 39, 59, 79, 99, 1 19, 139, 159,

179, 199, 219, 239, 259, 279, 299, 319 or 339, wherein the tryptophan is D- tryptophan

377. The peptide according to any of items 19, 39, 59, 79, 99, 1 19, 139, 159,

179, 199, 219, 239, 259, 279, 299, 319 or 339, wherein the tryptophan is L- tryptophan

378. The peptide according to any of items 20, 40, 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, 300, 320 or 340, wherein the tyrosine is D- tyrosine

379. The peptide according to any of items 20, 40, 60, 80, 100, 120, 140, 160,

180, 200, 220, 240, 260, 280, 300, 320 or 340, wherein the tyrosine is L- tyrosine 380. The peptide according to any of items 21 , 41 , 61 , 81 , 101 , 121 , 141 , 161 ,

181 , 201 , 221 , 241 , 261 , 281 , 301 , 321 or 341 , wherein the valine is D-valine

381. The peptide according to any of items 21 , 41 , 61 , 81 , 101 , 121 , 141 , 161 , 181 , 201 , 221 , 241 , 261 , 281 , 301 , 321 or 341 , wherein the valine is L-valine

382. The peptide according to item 1 to 381 , wherein one or more of said amino acid residues are modified, such as post-translationally modified or co- translationally modified

383. The peptide according to item 382, wherein said modification is acetylation of one or more amino acid residues

384. The peptide according to item 382, wherein said modification is phosphorylation of one or more amino acid residues 385. The peptide according to item 382, wherein said modification is glycosylation of one or more amino acid residues

386. The peptide according to item 382, wherein said modification is non- enzymatic glycosylation (or glycation) of one or more amino acid residues 387. The peptide according to item 382, wherein said modification is methylation of one or more amino acid residues

388. The peptide according to item 382, wherein said modification is amidation of one or more amino acid residues

389. The peptide according to item 382, wherein said modification is deamidation of one or more amino acid residues

390. The peptide according to item 382, wherein said modification is succinimide formation of one or more amino acid residues

391. The peptide according to item 382, wherein said modification is biotinylation of one or more amino acid residues 392. The peptide according to item 382, wherein said modification is formylation of one or more amino acid residues

393. The peptide according to item 382, wherein said modification is carboxylation of one or more amino acid residues

394. The peptide according to item 382, wherein said modification is carbamylation of one or more amino acid residues

395. The peptide according to item 382, wherein said modification is hydroxylation of one or more amino acid residues

396. The peptide according to item 382, wherein said modification is iodination of one or more amino acid residues 397. The peptide according to item 382, wherein said modification is isoprenylation (or prenylation or lipidation or lipoylation) of one or more amino acid residues

398. The peptide according to item 382, wherein said modification is GPI (glycosyl phosphatidylinositol) anchor formation of one or more amino acid residues

399. The peptide according to item 382, wherein said modification is myristoylation of one or more amino acid residues

400. The peptide according to item 382, wherein said modification is farnesylation of one or more amino acid residues 401 . The peptide according to item 382, wherein said modification is geranylgeranylation of one or more amino acid residues

402. The peptide according to item 382, wherein said modification is covalent attachment of nucleotides or derivates thereof to one or more amino acid residues

403. The peptide according to item 382, wherein said modification is ADP- ribosylation of one or more amino acid residues

404. The peptide according to item 382, wherein said modification is flavin attachment to one or more amino acid residues 405. The peptide according to item 382, wherein said modification is oxidation of one or more amino acid residues

406. The peptide according to item 382, wherein said modification is oxidative deamination of one or more amino acid residues

407. The peptide according to item 382, wherein said modification is deamination of one or more amino acid residues

408. The peptide according to item 382, wherein said modification is palmitoylation of one or more amino acid residues

409. The peptide according to item 382, wherein said modification is pegylation of one or more amino acid residues 410. The peptide according to item 382, wherein said modification is attachment of phosphatidyl-inositol of one or more amino acid residues

41 1. The peptide according to item 382, wherein said modification is phosphopantetheinylation of one or more amino acid residues

412. The peptide according to item 382, wherein said modification is polysialylation of one or more amino acid residues

413. The peptide according to item 382, wherein said modification is sulfation of one or more amino acid residues

414. The peptide according to item 382, wherein said modification is selenoylation of one or more amino acid residues 415. The peptide according to item 382, wherein said modification is arginylation of one or more amino acid residues

416. The peptide according to item 382, wherein said modification is glutamylation or polyglutamylation of one or more amino acid residues

417. The peptide according to item 382, wherein said modification is glycylation or polyglycylation of one or more amino acid residues 418. The peptide according to item 382, wherein said modification is acylation (or alkanoylation) of one or more amino acid residues

419. The peptide according to item 382, wherein said modification is Methylidene-imidazolone (MIO) formation of one or more amino acid residues 420. The peptide according to item 382, wherein said modification is p-

Hydroxybenzylidene-imidazolone formation of one or more amino acid residues

421. The peptide according to item 382, wherein said modification is Lysine tyrosyl quinone (LTQ) formation of one or more amino acid residues

422. The peptide according to item 382, wherein said modification is Topaquinone (TPQ) formation of one or more amino acid residues

423. The peptide according to item 382, wherein said modification is Porphyrin ring linkage of one or more amino acid residues

424. The peptide according to item 382, wherein said modification is glypiation (addition of glycosyl phosphatidyl inositol) of one or more amino acid residues 425. The peptide according to item 382, wherein said modification is addition of heme to one or more amino acid residues

426. The peptide according to item 382, wherein said modification is ubiquitination of one or more amino acid residues

427. The peptide according to item 382, wherein said modification is SUMOylation (Small Ubiquitin-like Modifier) of one or more amino acid residues

428. The peptide according to item 382, wherein said modification is ISGylation of one or more amino acid residues

429. The peptide according to item 382, wherein said modification is citrullination (or deimination) of one or more amino acid residues 430. The peptide according to item 382, wherein said modification is the formation of pyroglutamic acid (or pidolic acid) of one or more amino acid residues

431. The peptide according to item 382, wherein said modification is formation of disulfide bridges (or disulfide bond or SS-bond or persulfide connection) between two amino acid residues

432. The peptide according to item 382, wherein said modification is formation of a desmosine cross-link between two or more amino acid residues

433. The peptide according to item 382, wherein said modification is transglutamination between two or more amino acid residues 434. The peptide according to item 1 , wherein any of X₁, X₂, X3, X₄, X5, Xe, X7, X₈, X₉, X₁₀, X₁₁, X₁₂, Xi3, Xi4, Xi5 and/or X₁₆ is an uncommon or modified amino acid

435. The peptide according to item 434, wherein said uncommon amino acid is acetylalanine

436. The peptide according to item 434, wherein said uncommon amino acid is acetylaspartic acid

437. The peptide according to item 434, wherein said uncommon amino acid is acetylcysteine 438. The peptide according to item 434, wherein said uncommon amino acid is acetylglutamic acid

439. The peptide according to item 434, wherein said uncommon amino acid is acetylglutamine

440. The peptide according to item 434, wherein said uncommon amino acid is acetylglycine

441. The peptide according to item 434, wherein said uncommon amino acid is acetylisoleucine

442. The peptide according to item 434, wherein said uncommon amino acid is acetyllysine 443. The peptide according to item 434, wherein said uncommon amino acid is acetylmethionine

444. The peptide according to item 434, wherein said uncommon amino acid is acetylproline

445. The peptide according to item 434, wherein said uncommon amino acid is acetylserine

446. The peptide according to item 434, wherein said uncommon amino acid is acetylthreonine

447. The peptide according to item 434, wherein said uncommon amino acid is acetyltyrosine 448. The peptide according to item 434, wherein said uncommon amino acid is acetylvaline

449. The peptide according to item 434, wherein said uncommon amino acid is acetyllysine

450. The peptide according to item 434, wherein said uncommon amino acid is acetylcysteine 451 . The peptide according to item 434, wherein said uncommon amino acid is alanine amide

452. The peptide according to item 434, wherein said uncommon amino acid is arginine amide 453. The peptide according to item 434, wherein said uncommon amino acid is asparagine amide

454. The peptide according to item 434, wherein said uncommon amino acid is aspartic acid amide

455. The peptide according to item 434, wherein said uncommon amino acid is cysteine amide

456. The peptide according to item 434, wherein said uncommon amino acid is glutamine amide

457. The peptide according to item 434, wherein said uncommon amino acid is glutamic acid amide 458. The peptide according to item 434, wherein said uncommon amino acid is glycine amide

459. The peptide according to item 434, wherein said uncommon amino acid is histidine amide

460. The peptide according to item 434, wherein said uncommon amino acid is isoleucine amide

461. The peptide according to item 434, wherein said uncommon amino acid is leucine amide

462. The peptide according to item 434, wherein said uncommon amino acid is lysine amide 463. The peptide according to item 434, wherein said uncommon amino acid is methionine amide

464. The peptide according to item 434, wherein said uncommon amino acid is phenylalanine amide

465. The peptide according to item 434, wherein said uncommon amino acid is proline amide

466. The peptide according to item 434, wherein said uncommon amino acid is serine amide

467. The peptide according to item 434, wherein said uncommon amino acid is threonine amide ..

468. The peptide according to item 434, wherein said uncommon amino acid is tryptophan amide

469. The peptide according to item 434, wherein said uncommon amino acid is tyrosine amide 470. The peptide according to item 434, wherein said uncommon amino acid is valine amide

471. The peptide according to item 434, wherein said uncommon amino acid is an amino acid alcohol

472. The peptide according to item 434, wherein said uncommon amino acid is Aminobenzoic Acid

473. The peptide according to item 434, wherein said uncommon amino acid is Aminobutyric Acid

474. The peptide according to item 434, wherein said uncommon amino acid is Aminocyanobutyric acid 475. The peptide according to item 434, wherein said uncommon amino acid is

Aminocyanopropionic acid

476. The peptide according to item 434, wherein said uncommon amino acid is Aminocyclohexanoic acid

477. The peptide according to item 434, wherein said uncommon amino acid is Aminocyclopropanoic acid

478. The peptide according to item 434, wherein said uncommon amino acid is Aminocylopentanoic acid

479. The peptide according to item 434, wherein said uncommon amino acid is Aminodecanoic acid 480. The peptide according to item 434, wherein said uncommon amino acid is

Aminododecanoic acid

481. The peptide according to item 434, wherein said uncommon amino acid is Aminohexanoic acid

482. The peptide according to item 434, wherein said uncommon amino acid is Aminoisobutyric acid

483. The peptide according to item 434, wherein said uncommon amino acid is Aminomethylbenzoic acid

484. The peptide according to item 434, wherein said uncommon amino acid is Aminomethylcyclohexanoic acid 485. The peptide according to item 434, wherein said uncommon amino acid is Aminononanoic acid

486. The peptide according to item 434, wherein said uncommon amino acid is Aminooctanoic acid 487. The peptide according to item 434, wherein said uncommon amino acid is

Aminophenylalanine

488. The peptide according to item 434, wherein said uncommon amino acid is Amino Salicylic acid

489. The peptide according to item 434, wherein said uncommon amino acid is 2-Amino-2-Thiazoline-4-carboxylic acid

490. The peptide according to item 434, wherein said uncommon amino acid is Aminoundecanoic acid

491. The peptide according to item 434, wherein said uncommon amino acid is Aminovaleric acid 492. The peptide according to item 434, wherein said uncommon amino acid is

4-Benzoylphenylalanine

493. The peptide according to item 434, wherein said uncommon amino acid is Biphenylalanine

494. The peptide according to item 434, wherein said uncommon amino acid is Bromophenylalanine

495. The peptide according to item 434, wherein said uncommon amino acid is gamma-Carboxyglutamic acid

496. The peptide according to item 434, wherein said uncommon amino acid is canavanine 497. The peptide according to item 434, wherein said uncommon amino acid is

Carnitine

498. The peptide according to item 434, wherein said uncommon amino acid is Chlorophenylalanine

499. The peptide according to item 434, wherein said uncommon amino acid is Chlorotyrosine

500. The peptide according to item 434, wherein said uncommon amino acid is Cine

501. The peptide according to item 434, wherein said uncommon amino acid is Citrulline -. o

502. The peptide according to item 434, wherein said uncommon amino acid is 4-Cyano-2-Aminobutyric acid

503. The peptide according to item 434, wherein said uncommon amino acid is Cyclohexylalanine 504. The peptide according to item 434, wherein said uncommon amino acid is

Cyclohexylglycine

505. The peptide according to item 434, wherein said uncommon amino acid is Diaminobenzoic acid

506. The peptide according to item 434, wherein said uncommon amino acid is 2,4-Diaminobutyric acid

507. The peptide according to item 434, wherein said uncommon amino acid is 2,3-Diaminopropionic acid

508. The peptide according to item 434, wherein said uncommon amino acid is Dibutylglycine 509. The peptide according to item 434, wherein said uncommon amino acid is

Diethylglycine

510. The peptide according to item 434, wherein said uncommon amino acid is Dihydrotryptophan

51 1. The peptide according to item 434, wherein said uncommon amino acid is Dipropylglycine

512. The peptide according to item 434, wherein said uncommon amino acid is Fluorophenylalanine

513. The peptide according to item 434, wherein said uncommon amino acid is formylmethionine 514. The peptide according to item 434, wherein said uncommon amino acid is formylglycine

515. The peptide according to item 434, wherein said uncommon amino acid is formyllysine

516. The peptide according to item 434, wherein said uncommon amino acid is farnesylcysteine

517. The peptide according to item 434, wherein said uncommon amino acid is hydroxyfarnesylcysteine

518. The peptide according to item 434, wherein said uncommon amino acid is Homoalanine 519. The peptide according to item 434, wherein said uncommon amino acid is Homoarginine

520. The peptide according to item 434, wherein said uncommon amino acid is Homoasparagine 521 . The peptide according to item 434, wherein said uncommon amino acid is

Homoaspartic acid

522. The peptide according to item 434, wherein said uncommon amino acid is Homoglutamic acid

523. The peptide according to item 434, wherein said uncommon amino acid is Homoglutamine

524. The peptide according to item 434, wherein said uncommon amino acid is Homoisoleucine

525. The peptide according to item 434, wherein said uncommon amino acid is Homophenylalanine 526. The peptide according to item 434, wherein said uncommon amino acid is

Homoserine

527. The peptide according to item 434, wherein said uncommon amino acid is Homotyrosine

528. The peptide according to item 434, wherein said uncommon amino acid is Hydroxyproline

529. The peptide according to item 434, wherein said uncommon amino acid is Hydroxylysine

530. The peptide according to item 434, wherein said uncommon amino acid is 2-lndanylglycine 531. The peptide according to item 434, wherein said uncommon amino acid is

2-lndolecarboxylic acid

532. The peptide according to item 434, wherein said uncommon amino acid is lndoleglycine

533. The peptide according to item 434, wherein said uncommon amino acid is lodophenylalanine

534. The peptide according to item 434, wherein said uncommon amino acid is lsonipecotic Acid

535. The peptide according to item 434, wherein said uncommon amino acid is Kynurenine 536. The peptide according to item 434, wherein said uncommon amino acid is β-(S-Benzyl)Mercapto-β,β-cyclopentamethylene propionic acid

537. The peptide according to item 434, wherein said uncommon amino acid is Methyltyrosine 538. The peptide according to item 434, wherein said uncommon amino acid is

Methylphenylalanine

539. The peptide according to item 434, wherein said uncommon amino acid is methylalanine

540. The peptide according to item 434, wherein said uncommon amino acid is trimethylalanine

541. The peptide according to item 434, wherein said uncommon amino acid is methylglycine

542. The peptide according to item 434, wherein said uncommon amino acid is methylmethionine 543. The peptide according to item 434, wherein said uncommon amino acid is methylphenylalanine

544. The peptide according to item 434, wherein said uncommon amino acid is dimethylproline

545. The peptide according to item 434, wherein said uncommon amino acid is dimethylarginine

546. The peptide according to item 434, wherein said uncommon amino acid is methylarginine

547. The peptide according to item 434, wherein said uncommon amino acid is methylasparagine 548. The peptide according to item 434, wherein said uncommon amino acid is methylglutamine

549. The peptide according to item 434, wherein said uncommon amino acid is methylhistidine

550. The peptide according to item 434, wherein said uncommon amino acid is trimethyllysine

551. The peptide according to item 434, wherein said uncommon amino acid is dimethyllysine

552. The peptide according to item 434, wherein said uncommon amino acid is methyllysine 553. The peptide according to item 434, wherein said uncommon amino acid is methylcysteine

554. The peptide according to item 434, wherein said uncommon amino acid is glutamic acid 5-methyl ester 555. The peptide according to item 434, wherein said uncommon amino acid is

Naphthylalanine

556. The peptide according to item 434, wherein said uncommon amino acid is Nipecotic acid

557. The peptide according to item 434, wherein said uncommon amino acid is Nitrophenylalanine

558. The peptide according to item 434, wherein said uncommon amino acid is Norleucine

559. The peptide according to item 434, wherein said uncommon amino acid is Norvaline 560. The peptide according to item 434, wherein said uncommon amino acid is

Octahydroindolecarboxylic acid

561. The peptide according to item 434, wherein said uncommon amino acid is ornithine

562. The peptide according to item 434, wherein said uncommon amino acid is Penicillamine

563. The peptide according to item 434, wherein said uncommon amino acid is Phenylglycine

564. The peptide according to item 434, wherein said uncommon amino acid is phosphocysteine 565. The peptide according to item 434, wherein said uncommon amino acid is phosphohistidine

566. The peptide according to item 434, wherein said uncommon amino acid is phosphoserine

567. The peptide according to item 434, wherein said uncommon amino acid is phosphothreonine

568. The peptide according to item 434, wherein said uncommon amino acid is phosphotyrosine

569. The peptide according to item 434, wherein said uncommon amino acid is phosphoarginine 570. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-adenosine)-tyrosine

571 . The peptide according to item 434, wherein said uncommon amino acid is phosphopantetheine-serine 572. The peptide according to item 434, wherein said uncommon amino acid is

(phospho-5'-RNA)-serine

573. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-adenosine)-lysine

574. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-guanosine)-lysine

575. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-DNA)-serine

576. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-RNA)-tyrosine 577. The peptide according to item 434, wherein said uncommon amino acid is

(phospho-5'-adenosine)-threonine

578. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-DNA)-tyrosine

579. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-DNA)-threonine

580. The peptide according to item 434, wherein said uncommon amino acid is (phospho-5'-uridine)-tyrosine

581. The peptide according to item 434, wherein said uncommon amino acid is 4-Phosphonomethylphenylalanine 582. The peptide according to item 434, wherein said uncommon amino acid is palmitoylcysteine

583. The peptide according to item 434, wherein said uncommon amino acid is palmitoyllysine

584. The peptide according to item 434, wherein said uncommon amino acid is palmitoylthreonine

585. The peptide according to item 434, wherein said uncommon amino acid is palmitoylserine

586. The peptide according to item 434, wherein said uncommon amino acid is palmitoylcysteine 587. The peptide according to item 434, wherein said uncommon amino acid is phycoerythrobilin-bis-cysteine

588. The peptide according to item 434, wherein said uncommon amino acid is phycourobilin-bis-cysteine 589. The peptide according to item 434, wherein said uncommon amino acid is pyrrolidone-5-carboxylic acid

590. The peptide according to item 434, wherein said uncommon amino acid is Pipericolic Acid

591. The peptide according to item 434, wherein said uncommon amino acid is Propargylglycine

592. The peptide according to item 434, wherein said uncommon amino acid is Pyridinylalanine

593. The peptide according to item 434, wherein said uncommon amino acid is pyroglutamic acid 594. The peptide according to item 434, wherein said uncommon amino acid is

Sarcosine

595. The peptide according to item 434, wherein said uncommon amino acid is Tert-Leucine

596. The peptide according to item 434, wherein said uncommon amino acid is Tetrahydoisoquinoline-3-carboxylic acid

597. The peptide according to item 434, wherein said uncommon amino acid is Thiazolidinecarboxylic acid

598. The peptide according to item 434, wherein said uncommon amino acid is Thyronine 599. The peptide according to item 434, wherein said uncommon amino acid is selenocysteine

600. The peptide according to item 434, wherein said uncommon amino acid is selenomethionine

601. The peptide according to item 434, wherein said uncommon amino acid is erythro-beta-hydroxyasparagine

602. The peptide according to item 434, wherein said uncommon amino acid is erythro-beta-hydroxyaspartic acid

603. The peptide according to item 434, wherein said uncommon amino acid is gamma-carboxyglutamic acid 604. The peptide according to item 434, wherein said uncommon amino acid is aspartic 4-phosphoric anhydride

605. The peptide according to item 434, wherein said uncommon amino acid is2'-[3-carboxamido-3-(trimethylammonio)propyl]-histidine 606. The peptide according to item 434, wherein said uncommon amino acid is glucuronoylglycine

607. The peptide according to item 434, wherein said uncommon amino acid is geranylgeranylcysteine

608. The peptide according to item 434, wherein said uncommon amino acid is myristoylglycine

609. The peptide according to item 434, wherein said uncommon amino acid is myristoyllysine

610. The peptide according to item 434, wherein said uncommon amino acid is cysteine methyl disulfide 61 1. The peptide according to item 434, wherein said uncommon amino acid is diacylglycerolcysteine

612. The peptide according to item 434, wherein said uncommon amino acid is isoglutamylcysteine

613. The peptide according to item 434, wherein said uncommon amino acid is cysteinylhistidine

614. The peptide according to item 434, wherein said uncommon amino acid is lanthionine

615. The peptide according to item 434, wherein said uncommon amino acid is mesolanthionine 616. The peptide according to item 434, wherein said uncommon amino acid is methyllanthionine

617. The peptide according to item 434, wherein said uncommon amino acid is cysteinyltyrosine

618. The peptide according to item 434, wherein said uncommon amino acid is carboxylysine

619. The peptide according to item 434, wherein said uncommon amino acid is carboxyethyllysine

620. The peptide according to item 434, wherein said uncommon amino acid is (4-amino-2-hydroxybutyl)-lysine 621 . The peptide according to item 434, wherein said uncommon amino acid is biotinyllysine

622. The peptide according to item 434, wherein said uncommon amino acid is lipoyllysine 623. The peptide according to item 434, wherein said uncommon amino acid is pyridoxal phosphate-lysine

624. The peptide according to item 434, wherein said uncommon amino acid is retinal-lysine

625. The peptide according to item 434, wherein said uncommon amino acid is allysine

626. The peptide according to item 434, wherein said uncommon amino acid is lysinoalanine

627. The peptide according to item 434, wherein said uncommon amino acid is isoglutamyllysine 628. The peptide according to item 434, wherein said uncommon amino acid is glycyllysine

629. The peptide according to item 434, wherein said uncommon amino acid is isoaspartylglycine

630. The peptide according to item 434, wherein said uncommon amino acid is pyruvic acid

631. The peptide according to item 434, wherein said uncommon amino acid is phenyllactic acid

632. The peptide according to item 434, wherein said uncommon amino acid is oxobutanoic acid 633. The peptide according to item 434, wherein said uncommon amino acid is succinyltryptophan

634. The peptide according to item 434, wherein said uncommon amino acid is phycocyanobilincysteine

635. The peptide according to item 434, wherein said uncommon amino acid is phycoerythrobilincysteine

636. The peptide according to item 434, wherein said uncommon amino acid is phytochromobilincysteine

637. The peptide according to item 434, wherein said uncommon amino acid is heme-bis-cysteine 638. The peptide according to item 434, wherein said uncommon amino acid is heme-cysteine

639. The peptide according to item 434, wherein said uncommon amino acid is tetrakis-cysteinyl iron 640. The peptide according to item 434, wherein said uncommon amino acid is tetrakis-cysteinyl diiron disulfide

641. The peptide according to item 434, wherein said uncommon amino acid is tris-cysteinyl triiron trisulfide

642. The peptide according to item 434, wherein said uncommon amino acid is tris-cysteinyl triiron tetrasulfide

643. The peptide according to item 434, wherein said uncommon amino acid is tetrakis-cysteinyl tetrairon tetrasulfide

644. The peptide according to item 434, wherein said uncommon amino acid is cysteinyl homocitryl molybdenum-heptairon-nonasulfide 645. The peptide according to item 434, wherein said uncommon amino acid is cysteinyl molybdopterin

646. The peptide according to item 434, wherein said uncommon amino acid is (8alpha-FAD)-cysteine

647. The peptide according to item 434, wherein said uncommon amino acid is (8alpha-FAD)-histidine

648. The peptide according to item 434, wherein said uncommon amino acid is (δalpha-FAD)-tyrosine

649. The peptide according to item 434, wherein said uncommon amino acid is dihydroxyphenylalanine 650. The peptide according to item 434, wherein said uncommon amino acid is topaquinone

651. The peptide according to item 434, wherein said uncommon amino acid is tryptophyl quinine

652. The peptide according to item 434, wherein said uncommon amino acid is (tryptophan)-tryptophyl quinone

653. The peptide according to item 434, wherein said uncommon amino acid is glycosylasparagine

654. The peptide according to item 434, wherein said uncommon amino acid is glycosylcysteine 655. The peptide according to item 434, wherein said uncommon amino acid is glycosylhydroxylysine

656. The peptide according to item 434, wherein said uncommon amino acid is glycosylserine 657. The peptide according to item 434, wherein said uncommon amino acid is glycosylthreonine

658. The peptide according to item 434, wherein said uncommon amino acid is glycosyltryptophan

659. The peptide according to item 434, wherein said uncommon amino acid is glycosyltyrosine

660. The peptide according to item 434, wherein said uncommon amino acid is asparaginyl-glycosylphosphatidylinositolethanolamine

661. The peptide according to item 434, wherein said uncommon amino acid is aspartyl-glycosylphosphatidylinositolethanolamine 662. The peptide according to item 434, wherein said uncommon amino acid is cysteinyl-glycosylphosphatidylinositolethanolamine

663. The peptide according to item 434, wherein said uncommon amino acid is glycyl-glycosylphosphatidylinositolethanolamine

664. The peptide according to item 434, wherein said uncommon amino acid is seryl-glycosylphosphatidylinositolethanolamine

665. The peptide according to item 434, wherein said uncommon amino acid is seryl-glycosylsphingolipidinositolethanolamine

666. The peptide according to item 434, wherein said uncommon amino acid is (phosphoribosyl dephospho-coenzyme A)-serine 667. The peptide according to item 434, wherein said uncommon amino acid is

(ADP-ribosyl)-arginine

668. The peptide according to item 434, wherein said uncommon amino acid is (ADP-ribosyl)-cysteine

669. The peptide according to item 434, wherein said uncommon amino acid is glutamyl-glycerylphosphorylethanolamine

670. The peptide according to item 434, wherein said uncommon amino acid is sulfocysteine

671. The peptide according to item 434, wherein said uncommon amino acid is sulfotyrosine 672. The peptide according to item 434, wherein said uncommon amino acid is bromohistidine

673. The peptide according to item 434, wherein said uncommon amino acid is bromophenylalanine 674. The peptide according to item 434, wherein said uncommon amino acid is triiodothyronine

675. The peptide according to item 434, wherein said uncommon amino acid is thyroxine

676. The peptide according to item 434, wherein said uncommon amino acid is bromotryptophan

677. The peptide according to item 434, wherein said uncommon amino acid is dehydroalanine

678. The peptide according to item 434, wherein said uncommon amino acid is dehydrobutyrine 679. The peptide according to item 434, wherein said uncommon amino acid is dehydrotyrosine

680. The peptide according to item 434, wherein said uncommon amino acid is seryl-imidazolinone glycine

681. The peptide according to item 434, wherein said uncommon amino acid is oxoalanine

682. The peptide according to item 434, wherein said uncommon amino acid is alanyl-imidazolinone glycine

683. The peptide according to item 434, wherein said uncommon amino acid is allo-isoleucine 684. The peptide according to item 434, wherein said uncommon amino acid is isoglutamyl-polyglycine

685. The peptide according to item 434, wherein said uncommon amino acid is isoglutamyl-polyglutamic acid

686. The peptide according to item 434, wherein said uncommon amino acid is aminovinyl-cysteine

687. The peptide according to item 434, wherein said uncommon amino acid is (aminovinyl)-methyl-cysteine

688. The peptide according to item 434, wherein said uncommon amino acid is cysteine sulfenic acid 689. The peptide according to item 434, wherein said uncommon amino acid is glycyl-cysteine

690. The peptide according to item 434, wherein said uncommon amino acid is hydroxycinnamyl-cysteine 691 . The peptide according to item 434, wherein said uncommon amino acid is chondroitin sulfate glucuronyl-galactosyl-galactosyl-xylosyl-serine

692. The peptide according to item 434, wherein said uncommon amino acid is dermatan sulfate glucuronyl-galactosyl-galactosyl-xylosyl-serine

693. The peptide according to item 434, wherein said uncommon amino acid is heparan sulfate glucuronyl-galactosyl-galactosyl-xylosyl-serine

694. The peptide according to item 434, wherein said uncommon amino acid is glycosyl-hydroxyproline

695. The peptide according to item 434, wherein said uncommon amino acid is hydroxy-arginine 696. The peptide according to item 434, wherein said uncommon amino acid is isoaspartyl-cysteine

697. The peptide according to item 434, wherein said uncommon amino acid is alpha-mannosyl-tryptophan

698. The peptide according to item 434, wherein said uncommon amino acid is mureinyl-lysine

699. The peptide according to item 434, wherein said uncommon amino acid is chondroitin sulfate-aspartic acid ester

700. The peptide according to item 434, wherein said uncommon amino acid is (6-FMN)-cysteine 701. The peptide according to item 434, wherein said uncommon amino acid is diphytanylglycerol diether-cysteine

702. The peptide according to item 434, wherein said uncommon amino acid is bis-cysteinyl bis-histidino diiron disulfide

703. The peptide according to item 434, wherein said uncommon amino acid is hexakis-cysteinyl hexairon hexasulfide

704. The peptide according to item 434, wherein said uncommon amino acid is cysteine glutathione disulfide

705. The peptide according to item 434, wherein said uncommon amino acid is nitrosyl-cysteine 706. The peptide according to item 434, wherein said uncommon amino acid is (ADP-ribosyl)-asparagine

707. The peptide according to item 434, wherein said uncommon amino acid is beta-methylthioaspartic acid 708. The peptide according to item 434, wherein said uncommon amino acid is

(lysine)-topaquinone

709. The peptide according to item 434, wherein said uncommon amino acid is hydroxymethyl-asparagine

710. The peptide according to item 434, wherein said uncommon amino acid is (ADP-ribosyl)-serine

71 1. The peptide according to item 434, wherein said uncommon amino acid is cysteine oxazolecarboxylic acid

712. The peptide according to item 434, wherein said uncommon amino acid is cysteine oxazolinecarboxylic acid 713. The peptide according to item 434, wherein said uncommon amino acid is glycine oxazolecarboxylic acid

714. The peptide according to item 434, wherein said uncommon amino acid is glycine thiazolecarboxylic acid

715. The peptide according to item 434, wherein said uncommon amino acid is serine thiazolecarboxylic acid

716. The peptide according to item 434, wherein said uncommon amino acid is phenyalanine thiazolecarboxylic acid

717. The peptide according to item 434, wherein said uncommon amino acid is cysteine thiazolecarboxylic acid 718. The peptide according to item 434, wherein said uncommon amino acid is lysine thiazolecarboxylic acid

719. The peptide according to item 434, wherein said uncommon amino acid is keratan sulfate glucuronyl-galactosyl-galactosyl-xylosyl-threonine

720. The peptide according to item 434, wherein said uncommon amino acid is selenocysteinyl molybdopterin guanine dinucleotide

721. The peptide according to item 434, wherein said uncommon amino acid is histidyl-tyrosine

722. The peptide according to item 434, wherein said uncommon amino acid is methionine sulfone 723. The peptide according to item 434, wherein said uncommon amino acid is dipyrrolylmethanemethyl-cysteine

724. The peptide according to item 434, wherein said uncommon amino acid is glutamyl-tyrosine 725. The peptide according to item 434, wherein said uncommon amino acid is glutamyl-poly-glutamic acid

726. The peptide according to item 434, wherein said uncommon amino acid is cysteine sulfinic acid

727. The peptide according to item 434, wherein said uncommon amino acid is trihydroxyphenylalanine

728. The peptide according to item 434, wherein said uncommon amino acid is (sn-1 -glycerophosphoryl)-serine

729. The peptide according to item 434, wherein said uncommon amino acid is thioglycine 730. The peptide according to item 434, wherein said uncommon amino acid is heme P460-bis-cysteine-tyrosine 731. The peptide according to item 434, wherein said uncommon amino acid is tris-cysteinyl-cysteine persulfido-bis-glutamato-histidino tetrairon disulfide trioxide 732. The peptide according to item 434, wherein said uncommon amino acid is cysteine persulfide

733. The peptide according to item 434, wherein said uncommon amino acid is Lactic acid (2-hydroxypropanoic acid)

734. The peptide according to any of items 434 to 733, wherein said uncommon amino acid is the L-enantiomer

735. The peptide according to any of items 434 to 733, wherein said uncommon amino acid is the D-enantiomer

In one embodiment the present invention relates to a MHC multimer such as a MHC dextramer such as a biotinylated MHC dextramer compsising one or more one the peptides, lipopeptide or polyepitope peptide disclosed in WO 03/000720. The disclosures of WO 03/000720 are hereby incorpated into this patent application by reference.

Polvepitope peptides In one embodiment the present invention relates to a MHC multimer such as a MHC dextramer such as a biotinylated MHC dextramer compsising one or more polyepitope peptides. These poly epitope peptides can comprise one or more of the peptides disclosed in this application.

Peptide derivatives such as lipopeptides

In one embodiment the present invention relates to a MHC multimer such as a MHC dextramer such as a biotinylated MHC dextramer compsising one or more lipopeptides. These lipopeptides can comprise one or more of the peptides disclosed in this application.

Preferred derivatives of the subject peptides include lipopeptides, wherein a lipid moiety is conjugated to the amino acid sequence, said lipid moiety known to act as an adjuvant (Jung et al., Angew Chem, lnt Ed Engl 10, 872,1985; Martinon et al., J Immunol 149, 3416,1992; Toyokuni ef al., JAm Chem Soc 1 16, 395,1994; Deprez, et a/., J Med Chem 38,459,1995; Sauzet et a/., Vaccine 13, 1339,1995; BenMohamed et al., Eur. J. Immunol. 27,1242,1997; Wiesmuller et al., Vaccine 7,29, 1989; Nardin et al., Vaccine 16,590,1998; Benmohamed, et al. Vaccine 18, 2843, 2000; and Obert, et al., Vaccine 16, 161 ,1998). Suitable lipopeptides show none of the harmful side effects associated with adjuvant formulations, and both antibody and cellular responses have been observed against lipopeptides. Several different lipid moieties are known for use in lipopeptide constructs. Exemplary lipid moieties include, but are not limited to, palmitoyl, myristoyl, stearyl and decanol groups or, more generally, any C2 to C30 saturated, monounsaturated, or polyunsaturated fatty acyl group is thought to be useful.

The lipoamino acid S-[2, 3-bis (palmitoyloxy) propyl] cysteine, also known as

Pam3Cys- OH (Wiesmuller et al_, Z. Physiol. Chem. 364 593,1983), is a synthetic version of the N-terminal moiety of Braun's lipoprotein that spans the inner and outer membranes of Gram negative bacteria. United States Patent No. 5,700,910 to Metzger et a/ (December 23,1997) describes several N-acyl-S- (2-hydroxyalkyl) cysteines for use as intermediates in the preparation of lipopeptides that are used as synthetic adjuvants, B lymphocyte stimulants, macrophage stiumulants, or synthetic vaccines. Metzger et al. also teach the use of such compounds as intermediates in the synthesis of Pam3Cys- OH (Wiesmuller et al., Z. Physiol. Chem. 364,593,1983), and of lipopeptides that comprise this lipoamino acid or an analog thereof at the N-terminus. According to Metzger et a/., the peptide moiety of the lipopeptides are conjugated to the lipoamino acid moiety by removal of the C-terminal protective groups in the lipoamino acid, and then using the resultant compound as a substrate for lipopeptide synthesis, such as in solid phase peptide synthesis. Pam3Cys has been shown to be capable of stimulating virus-specific cytotoxic T lymphocyte (CTL) responses against influenza virus-infected cells (Deres et al., Nature 342,561 ,1989) and to elicit protective antibodies against foot-and-mouth disease (Wiesmuller et al., Vaccine 7,29,1989; United States Patent No. 6,024,964 to Jung et a/., February 15,2000) when coupled to the appropriate synthetic CTL epitopes. The advantage of using Pam3Cys in such vaccines is that the compound is a membrane anchor compound (i. e. it can penetrate into the plasma membrane of a cell to enhance the induction of cytotoxic T- lymphocytes in response to specific CTL epitopes attached via their N-termini to the lipoamino acid.

PamzCys, a synthetic lipoamino acid comprising the lipid moiety of macrophage- activating lipopeptide (i. e. MALP-2), has been recently synthesized (Metzger et a/., J Pept. Sci 1 , 184, 1995). Pam2Cys is reported to be a more potent stimulator of splenocytes and macrophages than Pam3Cys (Metzger et al., J Pept Sci 1 , 184, 1995 ; Muhlradt et al., J Exp Med 185, 1951 ,1997; and Muhlradt et a/., Infect lmmun 66, 4804,1998).

Alternatively, or in addition to the conjugation of one or more lipid moieties to the epitope of the invention, the epitopes are modified by the addition of one or more other epitopes, such as, for example, one or more HCMV B cell epitopes, HCMV T-helper epitopes or promiscuous/permissive T-helper epitopes. In this respect, the generation of an antibody response against a given antigen requires the generation of a strong T helper cell response (Vitiello et al., J. Clin. Invest. 95,341 -349,1995; Livingston et aL, J. Immunol. 159, 1383-1392,1997). Accordingly, it is particularly preferred to derivatize the subject CTL epitopes in this manner. Alternatively, promiscuous or permissive T- helper epitope-containing peptides are administered in conjunction with the antigen. Examples of promiscuous or permissive T-helper epitopes are tetanus toxoid peptide, Plasmodium falciparum pfg27, lactate dehydrogenase, and HIVgpi 20 (Contreas et al., Infect, lmmun, 66,3579-3590,1998; Gaudebout et al., J. A. I. D. S. Human Retroviral 14, 91 -101 ,1997; Kaumaya et al., J. MoI. Recog. 6,81 -94,1993; and Fern and Good J. Immunol. 148, 907-913,1992). Ghosh et al., Immunol 104, 58- 66,2001 and International Patent Application No. PCT/AUOO/00070 (WO 00/46390) also describe T- helper epitopes from the fusion protein of Canine Distemper Virus (CDV-F). Certain promiscuous T-helper epitopes induce strong B cell responses to a given antigen, and can bypass certain haplotype restricted immune responses (Kaumaya eta/., J. MoI. Recog. 6,81 -94,1993). Alternatively, or in addition, the peptide is derivatized by covalent linkage to an adjuvant which is known for immunization purposes, such as, for example, muralydipeptide, lipid or lipopolysaccharide.

Figure legends

Figure 1 : Schematic representation of MHC multimer. A MHC multimer consist of a multimerization domain whereto one or more MHC- peptide complexes are attached through one or more linkers. The multimerization domain comprice one or more carriers and/or one or more scaffolds. The MHC-peptide complexes comprice a peptide and a MHC molecule.

Figure 2: Program for peptide sequence motifs prediction

Figure 3: Full List of HLA Class I alleles assigned as of January 2007 from http://www.anthonynolan.org.uk/HIG/lists/class1 list.html

Figure 4: Top 30 HLA class 1 alleles in human ethnic groups

Figure 5: Reactive groups and the bonds formed upon their reaction.

Figure 6: Cleavable linkers, conditions for cleaving them and the resulting products of the cleavage.

Figure 7: Size exclusion chromatography of folded HLA-A*0201-β2m - QLFEELQEL (SEQ ID NO 9668) peptide-complex.

Purification of HLA-A^*0201 -β2m -QLFEELQEL (SEQ ID NO 9668) peptide-complex by size exclusion chromatography on a HiLoad 16/60 Superdex 75 column. Eluted protein was followed by measurement of the absorbance at 280 nm. The elution profile consisted of 4 peaks, corresponding to aggregated Heavy Chain, correctly folded MHC-complex, β2m and excess biotin and peptide. Figure 8: MHC-SHIFT Assay.

The SHIFT Assay shows that heavy chain is efficiently biotinylated, since the band corresponding to biotinylated heavy chain (lane 2) is shifted up-wards upon incubation with streptavidin. Lane 1 : Benchmark protein-ladder

Lane 2: Folded HLA-A^*0201 -β2m -QLFEELQEL (SEQ ID NO 9668) peptide-complex. Lane 3: Folded HLA-A^*0201 -β2m -QLFEELQEL (SEQ ID NO 9668) peptide-complex incubated with molar excess Streptavidin.

Figure 9: Composition of Fluorescein-linker molecule.

(A) Schematic representation of an example of a Fluorescein-linker molecule. (B) Composition of a L15 linker.

Figure 10: HLA alleles of the NetMHC databases List of the 24 MHC class 1 alleles used for peptide prediction by the database http://www.cbs.dtu.dk/services/NetMHC/ and the 14 MHC class 2 alleles used for peptide prediction by the database http://www.cbs.dtu.dk/services/NetMHCII/

Figure 11 : Ex vivo ELISPOT analysis of BclX(L)-specific CD8 positive T cells in PBL from a breast cancer patient.

Ex vivo ELISPOT analysis of BclX(L)-specific, CD8 positive T cells in PBL from a breast cancer patient either with or without the BcIX(L) YLNDHLEPWI (SEQ ID NO 9669) peptide. Analysis were performed in doublets and number of IFN-gamma producing T-cells are presented. (Reference: Sorensen RB, Hadrup SR, Kollgaard T, Svane IM, Thor Straten P, Andersen MH (2006) Efficient tumor cell lysis mediated by a BcI-X(L) specific T cell clone isolated from a breast cancer patient.Cancer Immunol lmmunother Apr;56(4)527-33)

Figure 12: PBL from a breast cancer patient analyzed by flow cytometry. PBL from a breast cancer patient was analyzed by flow cytometry to identify BcI-

X(L)173-182 (peptide YLNDHLEPWI (SEQ ID NO 9669)) specific CD8 T cells using the dextramer complex HLA-A2/Bcl-X(L)173-182-APC, 7-AAD-PerCP, CD3-FITC, and CD8-APC-Cy7. The dextramer complex HLA-A2/HIV-1 pol476-484-APC was used as negative control. -.

(Reference: Sorensen RB, Hadrup SR, Kollgaard T, Svane IM, Thor Straten P, Andersen MH (2006) Efficient tumor cell lysis mediated by a BcI-X(L) specific T cell clone isolated from a breast cancer patient.Cancer Immunol lmmunother Apr;56(4)527- 33)

Figure 13: 51-Cr release assay of isolated T cell clones. Ten expanded T cell clones isolated by Flow sorting and then expanded were tested for their specificity by analysis in a standard 51 -Cr release assay. For this purpose, T2 cells loaded with either BcI-X(L)173-182, YLNDHLEPWI (SEQ ID NO 9669) peptide or an irrelevant peptide (BA4697-105, GLQHWVPEL (SEQ ID NO 9670)) were used as target cells.

Figure 14: BcI-X(L)173-182 specific clone tested for its cytotoxic potential in 51 Cr-release assays.

A BcI-X(L)173-182 specific clone was tested for its cytotoxic potential in 51 Cr-release assays. Two assays were performed a Cell lysis of T2 cells pulsed with BcI-X(L)173-

182 peptide or an irrelevant peptide (BA4697-105, GLQHWVPEL (SEQ ID NO 9670)) in three E:T ratios, b Cell lysis of T2 cells pulsed with different concentrations of BcI-

X(L)173-182 peptide at the E:T ratio 1 :1

(Reference: Sorensen RB, Hadrup SR, Kollgaard T, Svane IM, Thor Straten P, Andersen MH (2006) Efficient tumor cell lysis mediated by a BcI-X(L) specific T cell clone isolated from a breast cancer patient.Cancer Immunol lmmunother Apr;56(4)527-

33)

Figure 15: Detection of CMV specific T cells using MHC dextramers. Dot plots showing live gated CD37CD4^' lymphocytes from CMV infected patient stained with (A) Negative Control MHC Dextramers (HLA-A^*0201 (GLAGDVSAV; (SEQ ID NO 9671 ))) or (B) MHC Dextramers containing peptides from CMV pp65 antigen (HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))).

Figure 16: Conformational ELISA. The ELISA is carried out as a sandwich-ELISA. The ELISA-plate was coated with W6/32 mouse-anti-hHLA-ABC (DAKO M0736) antibody, which recognizes a conformational epitope on correctly folded MHC-complex. Then MHC complex in various concentration was added. β2m in various concentrations was used as negative control. HRP-conjugated rabbit anti-β2m (DAKO P0174) was used for detection of bound MHC complex. TMB One-step substrate system (Dako) was used as a substrate for HRP, and color formation was followed by measurement of absorbance at 450 nm.

Figure 17. Carboxylate-modified beads coupled to TCR and stained with HLA- A*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE or HLA-A*0201 (ILKEPVHGV (SEQ ID

NO 9673))/RPE dextramers.

TCR in various concentrations were coupled to carboxylate- modified beads and then stained with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE or HLA- A^*0201 (ILKEPVHGV (SEQ ID NO 9673))/RPE dextramers in a flow cytometry experiment.

A) Histogram showing x-axis: Fluorescence intensity measured in the RPE channel (FL2), y-axis: events counted. Events measured in the Region R9 are regarded as negative, and events measured in Region R10 are regarded as positive.

B) Percentage of positively stained beads is shown for each preparation of beads. Negative control samples:

1 ) Beads coupled with 10 μg TCR stained with HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673))/RPE

2) Beads coupled with 0 μg TCR stained with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE Positive control samples:

3) Beads coupled with 2 μg TCR stained with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE

4) Beads coupled with 5 μg TCR stained with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE 5) Beads coupled with 10 μg TCR stained with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE

6) Beads coupled with 20 μg TCR stained with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE --OO

Figure 18: Flow cytometry analysis of human cell samples added TCR-COated beads. TCR-beads were added into human peripheral whole blood (left panel) or HPBMC (right panel) and then the samples were analysed by flow cytometry. Region R1 represents TCR-beads; region R2 represents lymphocyte cell population of interest.

Figure 19: Flow cytometry analysis of MHC multimer constructs carrying nonsense peptides.

Human Peripheral Blood Lymphocytes were ficoll purified from blood from a human donor and stained with mouse anti-human CD3/PE antibody and mouse anti-human CD8/PB antibody together with either of the MHC Dextramer molecule constructs A) HI_A-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/APC, B) HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673))/APC, C) HLA-A^*0201 (nonsense peptide 1 )/APC or D) HLA- A^*0201 (nonsense peptide 2)/APC. The staining was analysed on a CyAn ADP flow cytometer. Live-gated and CD3 positive lymphocytes are shown.

Figure 20: Summary of flow cytometry analysis of the binding of different MHC multimer constructs to specific T cells in purified Human Peripheral Blood. Mononuclear Cell samples. Purified HPBMC were stained with different MHC(peptide) molecules attached to APC labeled dextran270 multimerization domain and analyzed by flow cytometry . See example 31 for details on experimental procedures. 5 different MHC(peptide) molecules were investigated. Construct 1 : H LA-A^*0201 (GLAGDVSAV (SEQ ID NO 9671 )), construct 2: HLA- A^*0201 (ALIAPVHAV (SEQ ID NO 9674)), construct 3: HLA-A^*0201 (NLVPMVATV; (SEQ ID NO 9672)), construct 4: HLA-A^*0201 (GLCTLVAML (SEQ ID NO 9675)) and construct 5: HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673)). A positive staining is symbolized with a (+) and is here defined as the identification of a distinct CD8 positive and MHC (peptide) positive population when visualized in a dot plot (as exemplified in Figure 15). Negative staining is symbolized wit a (-) and is defined as absence of a distinct CD8 positive and MHC (peptide) positive population when visualized in a dot plot. Nt means not determined. All samples have previously been analyzed for the presence of T-cells restricted by HLA-A^*0201 (NLVPMVATV) (SEQ ID NO 9672), HLA- A^*0201 (GLCTLVAML (SEQ ID NO 9675)) and HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673)) and these results are shown in italics in the figure (column 2 and 3). Figure 21 : Gating strategy for no-lyse no-wash procedure.

Whole blood was stained with MHC multimer, anti-CD8/APC, anti-CD3/PB and CD45/CY antibody in a no-lyse no-wash procedure. For further details see text in example 28. During analysis of data the following gating strategy was used: CD45/PB antibody was used to set a trigger discriminator to allow the flow cytometer to distinguish between red blood cells and stained white blood cells. This was done during data collection by gating on CD45/PB positive cells in a CD45/PB vs. side scatter dot plot as shown in A. After data collection and during data analysis CD3 positive cells were selected by gating CD3/FITC positive cells in a CD3/FITC vs side scatter plot as shown in B. The final data was illustrated in a MHC multimer/PE vs CD8/APC plot (see Figure 22).

Figure 22: Identification of CMV-specific T cells in a blood sample using no-lyse no-wash procedure.

Whole blood from three different donors were analysed for the presence of CMV- specific T cells by flow cytometry using a no-lyse no-wash procedure. Donor 1 was stained with a MHC multimer consisting of PE-conjugated 270 kDa dextran coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide NLVPMVATV (SEQ ID NO 9672) derived from Human Cytomegalo Virus (HCMV) (left panel) and with a negative control MHC multimer consisting of PE conjugated 270 kDa dextran coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide ILKEPVHGV (SEQ ID NO 9673) derived from Human Immunodeficiency Virus (HIV) (right panel). Donor 2 was stained with a MHC multimer consisting of PE-conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide VTEHDTLLY (SEQ ID NO 9676) derived from Human Cytomegalo Virus (HCMV) (left panel) and a negative control MHC multimer consisting of PE-conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide IVDCLTEMY (SEQ ID NO 9677) derived from ubiquitin specific peptidase 9 (USP9) (right panel). Donor 3 was stained with twoMHC multimers consisting of PE conjugated 270 kDa dextran coupled with HLA-B^*0207 in complex with beta2microglobulin and either of the peptides TPRVTGGGAM (SEQ ID NO 9678) (left panel) or RPHERNGFTVL (SEQ ID NO 9679) (center panel) both derived from Human Cytomegalo Virus (HCMV) and with a negative control MHC multimer consisting of PE- conjugated 270 kDa dextran coupled with HLA- B^*0207 in complex with beta2microglobulin and the peptide TPGPGVRYPL (SEQ ID NO 9680) derived from Human Immunodeficiency Virus (HIV) (right panel).

All samples were also added Anti-CD45/PB, anti-CD3/FITC and anti-CD8/APC antibodies. The samples were gated as shown in Figure 21.

Figure 23: Enumeration of specific T cells using CytoCount™ beads.

Whole blood from a human donor were analysed for the presence of CMV-specific T cells with MHC multimers by flow cytometry using a no-lyse no-wash procedure. 2 x 100 μl donor blood was analysed with two different MHC multimers: A) PE-conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide VTEHDTLLY (SEQ ID NO 9676) derived from Human Cytomegalo Virus (HCMV) and a negative control construct B) consisting of PE-conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide IVDCLTEMY (SEQ ID NO 9677) derived from ubiquitin specific peptidase 9 (USP9). To each sample Anti-CD45/CY, anti-CD3/APC and anti-CD8/PB antibody was added together with 50 μl CytoCount beads (1028 beads/μl). Following staining for 15 minutes PBS was added to 1 ml and the samples analysed on a CyAn flow cytometer. During analysis CD45/CY antibody was used to set a trigger discriminator to allow the flow cytometer to distinguish between red blood cells and stained white blood cells and CD3/APC antibody was used to gate for CD3 positive T lymphocytes.

Amount of counted beads in sample A are shown in the histogram C and amount of beads counted in the negative control sample B are show in histogram D. Concentration of HLA-A^*0101 (VTEHDTLLY) (SEQ ID NO 9676) specific T cells in the blood sample was determined as follows: ((count of MHC multimer+ CD8+ cells in A x concentration of beads x dilution factor of beads) /counted beads C))- ((counted MHC multimer+ CD8+ cells in B x concentration of beads x dilution factor of beads) /counted beads D) = ((1300 cells x 1028 beads/μl x 0,05) / 67225 beads) - ((2 cells x 1028 beads/μl x 0,05) / 72623 beads) = 0,9926 cells/ μl = 992,6 celler/ml

Figure 24: MHC dextramers can be embedded in a sugar matrix together with antibodies and used for detection of specific T cells in a blood sample.

MHC dextramer constructs was embedded in a sugar matrix together with relevant gating reagents (anti-CD3/Pacific Blue, anti-CD8/Alexa700 and anti-CD45/Cascade Yellow antibodies) and the matrix dried. Then EDTA stabilized blood from a human donor were added and the samples analyzed by flow cytometry. Two different MHC construct were used HLA-A^*0101 (VTEHDTLLY (SEQ ID NO 9676))/PE dextramer (A) and the negative control construct HLA-A^*0101 (IVDCLTEMY (SEQ ID NO 9677) )/PE (B). As a control antibodies and MHC dextramer constructs were used to stain blood from the same donor following a general staining procedure without embedding the antibodies and MHC dextramers in a sugar matrix as described elsewhere herein. (C) Staining with HLA-A^*0101 (VTEHDTLLY (SEQ ID NO 9676) )/PE dextramer following a normal staining procedure and (D) Staining with HLA-A^*0101 (IVDCLTEMY (SEQ ID NO 9677))/PE dextramer following a normal staining procedure.

Figure 25: Summary flow chart, ELISPOT summary flow chart showing measurement of antigen reactive T-CeIIs by IFN-γ capture in blood samples by ELISPOT. See example 37 for more detailed information.

Figure 26: Detection of activated lymphocytes using MHC pentamers and IFN-γ.

The figures illustrate IFN-γ versus MHC Pentamer staining of live lymphocytes. PBMCs were incubated with either a negative control (non-specific) Pentamer (A^*0201/EBV (GLCTLVAML (SEQ ID NO 9675))) or a Pentamer specific for the cells of interest (B^*0801/EBV (RAKFKQLL)), then stimulated with LAC (non-specific activation) or B^*0801/EBV peptide (specific peptide activation) for 15 hours in the presence of Brefeldin A. Fixation, permeabilization and staining for IFN-γ were carried out exactly as detailed in the protocol. From www.proimmune.com: Pro5 Recombinant MHC Pentamer staining protocol for human Intracellular Proteins. Version 4.1 02/2007.

Figure 27. SDS PAGE shift assay with SA and B*0802-Cys Dextramers. 1 ug Maleimide-biotin treated B^*0802-cys-peptide complex was incubated with 1.8 ug SA in 12 ul PBS, pH 7.0 for 1 hour at room temperature. Then the sample was analyzed by SDS PAGE together with samples of various concentrations of Maleimide- biotin treated B^*0802-cys-peptide complex not incubated with SA and a sample with SA alone .

Lane 1 and 1 1 : Marker, Benchmark. Lane 2: 1 ug B^*0802-cys (ELRRKMMYM) + SA, Lane 3: 1 ug B^*0802-cys (ELRRKMMYM), Lane 4: 0.5 ug B^*0802-cys (ELRRKMMYM), Lane 5: 0.25 ug B^*0802-cys (ELRRKMMYM), Lane 6: 1 ug B^*0802-cys (AAKGRGAAL) + SA, Lane 7: 1 ug B^*0802-cys (AAKGRGAAL), Lane 8: 0.5 ug B^*0802-cys (AAKGRGAAL), Lane 9: 0.25 ug B^*0802-cys (AAKGRGAAL), Lane 10: SA.

Figure 28. Detection of CMV-specific T cells with Chemically biotinylated MHC Dextramers.

Human Peripheral Blood Lymphocytes were ficoll purified from blood from a human donor and stained with mouse anti-human CD3/APC antibody, mouse-anti-human CD4/FITC antibody and mouse anti-human CD8/PB antibody together with either A) the CMV specific MHC Dextramer B^*0802-Cys(ELRRKMMYM)/PE or B) the negative control MHC Dextramer B^*0802-Cys(AAKGRGAAL )/PE. The staining was analysed on a CyAn ADP flow cytometer. Live-gated, CD3 positive and CD4 negative lymphocytes are shown.

Figure 29. Detection of CMV-specific T cells with Chemically biotinylated MHC Dextramers.

Human Peripheral Blood Lymphocytes were ficoll purified from blood from a human donor and stained with mouse anti-human CD3/APC antibody, mouse-anti-human CD4/FITC antibody and mouse anti-human CD8/PB antibody together with either A) the CMV specific MHC Dextramer A^*0101 -Cys(VTEHDTLLY)/PE or B) the negative control MHC Dextramer B^*0802-Cys(AAKGRGAAL )/PE. The staining was analysed on a CyAn ADP flow cytometer. Live-gated, CD3 positive and CD4 negative lymphocytes are shown.

Figure 30. Detection of CMV-specific T cells with Chemically biotinylated MHC Dextramers.

Human Peripheral Blood Lymphocytes were ficoll purified from blood from a human donor and stained with mouse anti-human CD3/APC antibody, mouse-anti-human CD4/FITC antibody and mouse anti-human CD8/PB antibody together with either A) the CMV specific MHC Dextramer B^*0702-Cys(RPHERNGFTVL)/PE or B) the negative control MHC Dextramer B^*0802-Cys(AAKGRGAAL )/PE. The staining was analysed on a CyAn ADP flow cytometer. Live-gated, CD3 positive and CD4 negative lymphocytes are shown.

Figure 31. Detection of CMV-specific T cells with Chemically biotinylated MHC Dextramers. Human Peripheral Blood Lymphocytes were ficoll purified from blood from a human donor and stained with mouse anti-human CD3/APC antibody, mouse-anti-human CD4/FITC antibody and mouse anti-human CD8/PB antibody together with either A) the CMV specific MHC Dextramer B^*0702-Cys(TPRVTGGGAM)/PE or B) the negative control MHC Dextramer B^*0802-Cys(AAKG RGAAL )/PE. The staining was analysed on a CyAn ADP flow cytometer. Live-gated, CD3 positive and CD4 negative lymphocytes are shown.

Examples

Example 1

This example describes how to make a MHC class I complex with a peptide in the peptide binding-groove using in vitro refolding. The MHC-complex in this example consisted of light chain β2m, the MHC class I Heavy Chain allele HLA-A^*0201 (a truncated version in which the intracellular and transmembrane domains have been deleted) and the peptide QLFEELQEL (SEQ ID NO 9668).

MHC l-complexes consists of 3 components; Light Chain (β2m), Heavy Chain and a peptide of typically 8-10 amino acids. In this example MHC-complexes was generated by in vitro refolding of heavy chain, β2m and peptide in a buffer containing reduced and oxidized glutathione. By incubation in this buffer a non-covalent complex between Heavy Chain, β2m and peptide was formed. Heavy chain and β2m was expressed as inclusion bodies in E.coli prior to in vitro refolding following standard procedures as described in Garboczi et al., (1996), Nature 384, 134-141 . Following refolding the MHC complexes was biotinylated using BirA enzyme able to biotinylate a specific amino acid residue in a recognition sequence fused to the C-terminal of the Heavy Chain by genetic fusion. Monomer MHC complexes was then purified by size exclusion chromatography.

1 . 200 ml of refolding buffer (100 mM Tris, 400 mM L-arginin-HCL, 2 mM NaEDTA, 0.5 mM oxidized Gluthathione, 5 mM reduced Glutathione, pH 8.0) was supplied with protease inhibitors PMSF (phenylmethylsulphonyl fluoride), Pepstatin A and Leupeptin (to a final concentration of 1 mM, 1 mg/l and 1 mg/l, respectively). The refolding buffer was placed at 10 ⁰C on a stirrer. 2. 12 mg of peptide QLFEELQEL (SEQ ID NO 9668) was dissolved in DMSO or another suitable solvent (300-500 μl), and added drop-wise to the refolding buffer at vigorous stirring.

3. 4.4 mg of human Light Chain β2m was added drop-wise to the refolding buffer at vigorous stirring.

4. 6.2 mg of Heavy Chain HLA-A^*0201 (supplied with DTT to a concentration of 0.1 mM) was added drop-wise to the refolding buffer at vigorous stirring.

5. The folding reaction was placed at 10⁰C at slow stirring for 4-8 hours.

6. After 4-8 hours, step 3 and 4 was repeated and the folding reaction is placed at 1 O⁰C at slow stirring O/N.

7. Step 3 and 4 was repeated, and the folding reaction is placed at 10⁰C at slow stirring for 6-8 hours.

Optionally, steps 5-7 may be done in less time, e.g. a total of 0.5-5 hours.

8. After 6-8 hours the folding reaction was filtrated through a 0.2 μm filter to remove aggregates.

9. The folding reaction was concentrated O/N at 10⁰C shaking gently in a suitable concentrator with a 5000 mw cut-off filter. The folding reaction was concentrated to approximately 5-10 ml. (Optionally the filtrate can be stored at 4⁰C and reused for another folding with the same peptide and heavy chain.) 10. The concentrated folding reaction was buffer-exchanged at least 8 times, into a

MHC-buffer (20 mM Tris-HCI, 50 mM NaCI, pH 8.0) and concentrated (at 1 O⁰C in a suitable concentrator with a 5000 mw cut-off filter) down to approximately 1 ml.

1 1. The heavy chain part of the MHC-complex was biotinylated by mixing the following components: approximately 1000 μl folded MHC-complex, 100 μl each of Biomix-A, Biomix-B and d-Biotin (all 3 from Biotin Protein Ligase Kit from Avidity, 10 μl birA enzyme (3 mg/ml, from Biotin Protein Ligase Kit from Avidity, 0.5 μl Pepstatin A (2 mg/ml) and 0.5 μl Leupeptin (2 mg/ml). The above was gently mixed and incubated O/N at room temperature.

12. The biotinylated and folded MHC-complex solution was centrifuged for 5 min. at 170Ox g, room temperature.

13. Correctly folded MHC-complex was separated and purified from excess biotin, excess β2m, excesss heavy chain and aggregates thereoff, by size exclusion chromatography on a column that separates proteins in the 10-100 kDa range. Correctly folded monomer MHC-complex was eluted with a MHC-buffer (20 mM Tris-HCI, 50 mM NaCI, pH 8.0). The elution profile consisted of 4 peaks, corresponding to aggregated Heavy Chain, correctly folded monomer MHC- complex, β2m and excess biotin and peptide (See figure 7).

14. Fractions containing the folded MHC-complex were pooled and concentrated to approximately 1 ml in a suitable concentrator with a 5000 mw cut-off filter. The protein-concentration was estimated from its abosorption at 280 nm.

15. Folded MHC-complex can optionally be stored stored at -17O⁰C before further use.

16. The grade of biotinylation was analyzed by a SDS PAGE SHIFT-assay with Streptavidin (figure 8) and correct folding was confirmed by ELISA, using the antibody W6/32 that recognizes correctly folded MHC-peptide complex.

The above procedure may be used for folding any MHC I compexes consisting of any β2m, any heavy chain and any peptide approx. 8-1 1 amino acids long. Either of the components can be truncated or otherwise modified. The above procedure can also be used for generation of "empty" MHC I complexes consisting of β2m and heavy chain without peptide.

Example 2

This example describes how to generate soluble biotinylated MHC Il complexes using a baculovirus expression system, where the MHC Il complex was DR4 consisting of the α-chain DRA1 ^*0101 and the β-chain DRB1 ^*0401 as described by Svendsen et al., (2004), J. Immunol. 173(1 1 ):7037-45. Briefly, The hydrophobic transmembrane regions of the DRa and DRβ chains of DR4 were replaced by leucine zipper dimerization domains from the transcription factors Fos and Jun to promote DR α/β assembly. This was done by ligating cytoplasmic cDNA sequences of DRA V0101 and DRBV0401 to fos- and yun-encoding sequences. A birA site GLNDIFEAQKIEWH (SEQ ID NO 9681 ) was added to the 3' end of the DRA V0101-fos template. Covalently bound peptide

AGFKGEQGPKGEP (SEQ ID NO 9682) derived from collagen Il amino acid 261-273 were genetically attached by a flexible linker peptide to the N terminus of the DRβ- chain. Finally, the modified DRA1 ^*0101 and DRB1 ^*0401 inserts were cloned into the expression vector pAcAb3. The pAcAB3-DRA1 ^*0101/ DRB1 ^*0401 plasmids were cotransfected with linearized baculovirus DNA (BD Pharmingen; BaculoGold kit) into Sf9 insect cells, according to the manufacturer's instructions. Following two rounds of plaque purification, clonal virus isolates were further amplified three times before preparation of high-titer virus (10⁸-10¹⁰AnI). These stocks were used to infect High Five or serum-free Sf21 insect cells (Invitrogen Life Technologies, Carlsbad, CA) for protein production. Spinner cultures (2-3 x 10⁶ cells/ml) were infected at a multiplicity of infection of 1-3 in a volume of 150 ml per 2 L spinner flask. Supernatantswere harvested and proteinase inhibitor tablets (Roche, Basel, Switzerland) were added before affinity purification on MiniLeak-Low columns (Kem-En-Tec) coupled with the anti-HLA-DR monoclonal antibody L243. HLA-DR4 complexes were eluted with diethylamine (pH 1 1 ) into neutralization buffer (2 M Tris, pH 6.5) and immediately buffer exchanged and concentrated in PBS, 0.01% NaN₃, using Millipore (Bedford, MA) concentrators. The purity of protein was confirmed by SDS-PAGE. The purified DR4 complexes were biotinylated in vitro as described for MHC I complexes elsewhere herein. These complexes may now be used for coupling to any dimerization domain, e.g. divynylsulfone activated dextran 270coupled with SA and a fluorochrome.

Example 3

This example describes how to generate empty biotinylated MHC Il complexes using a baculovirus expression system , where the MHC Il complex consist of any α-chain and any β-chain, including truncated and otherwise modified versions of the two. Briefly, The hydrophobic transmembrane regions of the DRa and DRβ chains of MHC Il are replaced by leucine zipper dimerization domains from the transcription factors Fos and Jun to promote DR α/β assembly. This is done by ligating cytoplasmic cDNA sequences of DRa and DRβ to fos- and yun-encoding sequences. A birA site

GLNDIFEAQKIEWH (SEQ ID NO 9681 ) is added to the 3' end of either the DRα-fos/ DRα-yun or the DRβ-yun/ DRβ-fos template. The modified DRa and DRβ inserts is cloned into the expression vector pAcAb3 and cotransfected with linearized baculovirus DNA into Sf9 insect cells, according to the manufacturer's instructions. Following rounds of plaque purification, clonal virus isolates is further amplified before preparation of high-titer virus. These stocks are used to infect High Five or serum-free Sf21 insect cells (Invitrogen Life Technologies, Carlsbad, CA) for protein production, e.g. as Spinner cultures. Supernatants are harvested and proteinase inhibitors added before affinity purification, e.g. using a MiniLeak-Low columns (Kem-En-Tec) coupled with anti-MHC Il antibody. The purified MHC Il complexes is biotinylated in vitro as described for MHC I complexes elsewhere herein. These biotinylated MHC Il complexes may now be used for coupling to any dimerization domain, e.g. divynylsulfone activated dextran 270coupled with SA and a fluorochrome.

Example 4 This example describes how to generate biotinylated MHC Il complexes using a cell based protein expression system , where the MHC Il complex consist of any α-chain and any β-chain, including truncated and otherwise modified versions of the two. The MHC Il complex may also have a peptide bound in the peptide binding cleft. The hydrophobic transmembrane regions of the MHC Il α-chain and MHC Il β-chain are replaced by leucine zipper dimerization domains from the transcription factors Fos and Jun to promote α/β chain assembly. This is done by ligating cytoplasmic cDNA sequences of α-chain and β-chain to fos- and yun-encoding sequences. A birA site GLNDIFEAQKIEWH (SEQ ID NO 9681 ) is added to the 3' end of the DRα-fos template. Optionally covalently bound peptide is genetically attached by a flexible linker peptide to the N terminus of the DRβ-chain. The modified DRa and DRβ inserts is cloned into a suitable expression vector and transfected into a cell line capable of protein expression, e.g. insect cells, CHO cells or similar. Transfected cells are grown in culture, supernatants are harvested and proteinase inhibitors added before affinity purification, e.g. using a MiniLeak-Low columns (Kem-En-Tec) coupled with anti-MHC Il antibody. Alternatively the expressed MHC Il complexes may be purified by anion- or cation-exchange chromatography. The purified MHC Il complexes is biotinylated in vitro as described for MHC I complexes elsewhere herein. These biotinylated MHC Il complexes may now be used for coupling to any dimerization domain, e.g. divynylsulfone activated dextran 270coupled with SA and a fluorochrome.

Example 5

This is an example of how to make a MHC multimer that is a tetramer and where the MHC are attached to the multimerization domain through a non-covalent interaction The multimerization domain consist of Streptavidin. The MHC molecule was biotinylated DR4 consisting of the α-chain DRA1 ^*0101 and the β-chain DRB1 ^*0401 and the peptide AGFKGEQGPKGEP (SEQ ID NO 9682) derived from collagen Il amino acid 261-273. The biotinylated MHC-peptide complexes was generated as described in a previous example herein. Fluorescent DR4-peptide tetramer complexes were assembled by addition of ultra- avidin-R-PE (Leinco Technologies, St. Louis, MO) at a final molar ratio of biotinylated to DR4-peptide ultra-avidin-R-PE of 6:1. The resulting DR4-peptide multimer complexes were subjected to size exclusion on a Superdex-200 column to separate the tetramer complexes from protein aggregates and lower molecular weight complexes and excess fre DR4-peptide. The tetramer complexes were concentrated using Centicon-30 concentrators and stored at 0.1 -0.3 mg/ml in a mixture of protease inhibitors.

These complexes could be used to detect specific T cells in a flow cytometry assay as described by Svendsen et al.(2004) Tracking of Proinflammatory Collagen-Specific T cells in Early and Late Collagen-Induced Arthritis in Humanized mice. J. Immunol. 173:7037-7045.

Example 6

This example describes how an activated divinylsylfone-dextran(270kDa)(VS-dex270) was coupled with streptavidin (SA) and Allophycocyanin (APC). Such molecules can be used as multimerization domains for attachment of biotinylated MHC molecules.

1 . Streptavidin (approx. 100 mg SAAnI in 10 mM HEPES, 0,1 M NaCI, pH 7.85) was dialysed with gentle stirring for 2 days against 10 mM HEPES, 0.1 M NaCI, pH 7.85 (20 fold excess volume) at 2-8⁰C with 1 buffer change/day.

2. 5 ml of APC from a homogen suspension (approx. 10 mg/ml) was centrifuged 40 min. at 3000 rpm. The supernatant was discharged and the precipitate dissolved in 5 ml of 10 mM HEPES, 0,1 M NaCI, pH 7.85. This APC solution was dialysed with gentle stirring in the dark for 2 days against 10 mM HEPES, 0.1 M NaCI, pH 7.85 (20 fold excess volume) at 2-8⁰C with 1 buffer change/day.

3. The APC-solution was concentrated to 1 ml and the concentration measured to 47 g/L at UV 650nm. The A650/A278-ratio was measured to 4.2.

4. The SA-solution was filtrated through a 0.45 μm filter and the protein concentration was measured to 61.8 g SA/L at UV 278nm . 5. Conjugation: The reagents was mixed to a total volume of 500 μl in the following order with 8.1 mol SA/mol Dex and 27 mol APC/mol Dex.: a) 90 μl water b) 160 μl activated VS-dex270 c) 23 μl SA (61 ,8 g/L) ~ 8.1 equivalents, d) 177 μl APC (47 g/L) ~ 27 equivalents, e) 50 μl of 100 mM HEPES, 1 M NaCI, pH 8

The reaction was placed in a water bath with stirring at 3O⁰C in the dark for 18 hours. 6. The coupling was stopped by adding 50 μl 0,1 M ethanolamine, pH 8.0. 7. The conjugate was purified on a Sephacryl S-200 column with 10 mM HEPES, 0,1 M NaCI buffer, pH 7.2.

8. 3 peaks were collected (peak 1 : APC-S A-dex270; peak 2: Free APC; peak 3: Free SA). Volume, UV A650 and UV A278 were measured. 9. The concentration of dextran270, APC/Dex and SA/Dex were calculated to

22.4x10⁸ M; 3.48 and 9.54 respectively.

10. The conjugate were added NaN₃ and BSA to a final concentration of 15 mM and 1 % respectively. The volume was adjusted with 10 mM HEPES, 0.1 M NaCI, pH 7.2 to a final concentration of 16x10⁸ M Dex270. 1 1. The conjugate were kept at 2-8⁰C in dark until further use.

The conjugate can be coupled with biotinylated MHC molecules to generate a MHC multimer as described in example 8.

Example 7

This example describes how an activated divinylsylfone-dextran(270kDa)(VS-dex270) was coupled with streptavidin (SA) and R-phycoerythrin (RPE). The coupling procedure described for coupling of SA and APC to VS-dex270 (as described in example 6) were followed with the exception that APC were replaced with RPE.

Example 8 This example describes how to couple an empty MHC or a MHC-complex to a dextran multimerization domain through a non-covalent coupling, to generate a MHC- dextramer. The MHC-dextramer in this example consisted of APC-streptavidin (APC- SA) -conjugated 27OkDA dextran and a biotinylated, folded MHC-complex composed of β2m, HLA-A^*0201 heavy chain and the peptide NLVPMVATV (SEQ ID NO 9672). The APC-SA conjugated 27OkDA dextran was generated as described in example 6 and contained 3,7 molecules of SA per dextran (each SA can bind 3 MHC-complexes) and the concentration was 16x10^'8 M. The concentration of the HLA-A^*0201/ NLVPMVATV(SEQ ID NO 9672)-complex was 4 mg/ml (1 μg = 20,663 pmol). The molecular concentration of the MHC-complex was 8,27x10⁵M . The MHC-complex was attached to the dextran by a non-covalent Biotin-Streptavidin interaction between the biotinylated Heavy Chain part of the MHC-complex and the SA, conjugated to dextran.

Here follows a protocol for how to produce 1000 μl of a MHC-dextramer solution with a final concentration of approximately 32x10⁹M :

1 . 200 μl_ 270 kDA vinylsulfone-activated dextran, corresponding to 3,2x10 ¹¹ mol, and 4 μl MHC-complex, corresponding to 3,55x10 ¹⁰ mol was mixed and incubated at room temperature in the dark for 30 min.

2. A buffer of 0,05M Tris-HCI, 15 mM NaN₃, 1 % BSA, pH 7,2 was added to a total volume of 1000 μl.

3. The resulting MHC-dextramer preparation may now be used in flow cytometry eksperiments.

Example 9

This is an example of how to make and use MHC multimers that are trimers consisting of a streptavidin multimerization domain with 3 biotinylated MHC complexs and 1 flourophore molecule attached to the biotin binding pockets of streptavidin. MHC complexs consisting of HLA-A^*0201 heavy chain, beta2microglobulin and NLVPMVATV (SEQ ID NO 9672) peptide or the negative control peptide GLAGDVSAV (SEQ ID NO 9671 ) were generated as described elsewhere herein. The fluorophore in this example was Fluorescein-linker molecules as shown in figure 9. Each of these molecules consist of a linker-biotin molecule mounted with 4 trippel fluorescein-linker molecules. The linker-biotin molecule was here H-L30-Lys(NH₂)-L30-Lys(NH₂)-L30- Lys(NH₂)L300Lys(caproylamidobiotin)-NH₂ where L30 was a 30 atom large linker and L300 was a 300 atom large linker. Both L30 and L300 was composed of multiple L15 linkers with the structure shown in figure 9B. Linker-biotin molecules were generated as follows: Downloaded Boc-L300-Lys(Fmoc) resin (100 mg) was deprotected and subjected to coupling with Boc-Lys(2CIZ)-OH, Boc-L30-OH, Boc-Lys(2CIZ)-OH, Boc- L30-OH, Boc-Lys(2CIZ)-OH then Boc-L30-OH. The resin was Fmoc deprotected and reacted twice (2x 2 h) with caproylamido biotin NHS ester (25 mg in 0.5 mL NMP + 25 microL DIPEA). The resin was washed with TFA and the product cleaved off with TFA:TFMSA:mCresol:thioanisol (6:2:1 :1 ), 1 mL, precipitated with diethyl ether and purified by RP-HPLC. MS calculated for C₃₀₀H₅₄₄N₆₄Oi₃₇S is 7272.009 Da, found 7271 .19 Da.

Alternatively linker-biotin molecule was H-L60- Lys(NH₂)-L60- Lys(NH₂)-L60- Lys(NH₂)L300Lys(caproylamidobiotin)-NH₂ and made from downloaded Boc-L300- Lys(Fmoc) resin (100 mg), and then prepared analogously to H-L30-Lys(NH₂)-L30- Lys(NH₂)-L30-Lys(NH₂)L300Lys(caproylamidobiotin)-NH₂. MS calculated for C₃₆₀H₆₅₂N₇₆Oi₆₇S is 8749.5848 Da and was found to be 7271.19 Da. Yield 3 mg. The trippel fluorescein-linker molecules was here betaalanin-L90-Lys(Flu)-L90- Lys(Flu)-L90-Lys(Flu)-NH₂ where Lys = Lysine, Flu = Fluorescein and L90 is a 90 atom linker (se figure 9 for further details). The trippel-fluorescein-linker molecule was generated as follows: Downloaded Boc-Lys(Fmoc) resin, 2 g,was Boc deprotected and subjected to 3 x coupling with Boc-L30-OH, Boc-Lys(Fmoc)-OH, 3 x Boc-L30-OH, Boc- Lys(Fmoc)-OH, 3 x Boc-L30-OH. The three Fmoc groups were removed and carboxyfluorescein, 301 mg, activated with HATU, 274 mg, and DIPEA, 139 μL, in 8 mL NMP, was added to the resin twice for 30 min. The resin was Boc deprotected and subjected to 2 x 30 min coupling with beta-alanine-N,N-diacetic acid benzyl ester, followed by 5 min treatment with 20 % piperidine in NMP. The resin was washed with DCM, then TFA and the product was cleaved off the resin, precipitated with diethyl ether and purified by RP-HPLC. Yield was 621 mg. MS calculated for C268H402N44O1 16 is 6096.384 Da, while MS found was 6096 Da.

Biotin-linker molecule were coupled together with 4 trippel fluorescein-linker molecules as follows: (500 nmol) was dissolved in 88 microliter NMP + 2 μl pyridine and activated for 10 min at room temperature (conversion to cyclic anhydride) by addition of 10 μl N, N' diisopropylcarbodiimide. Following activation the trippel fluorescein-linker was precipitated with diethyl ether and redissolved in 100 microliter NMP containing 10 nmol biotin-linker. Once dissolved the coupling was initiated by addition of 5 μl diisopropyl ethyl amine, and was complete after 30 min.

Streptavidin and Fluorescein-linker molecules are then mixed in a molar ration of 1 :1 and incubated for Vz hour. Then MHC complexes are added in 3-fold molar excess in respect to streptavidin and incubated for another Vz hour. Alternatively, MHC complexes are added first, then Fluorescein-linker molecules or MHC complexes are mixed with Fluorescein-linker molecules before addition to Streptavidin. These MHC multimers are then used to stain CMV specific T cells in a flow Cytometry experiment. 1x10⁶ purified HPBMC from a donor with T cells specific for HLA- A^*0201 (NLVPMVATV(SEQ ID NO 9672)) are incubated with 10 μl of each of the two HLA-A^*0201 (peptide)/Fluorescein constructs described above for 10 minutes in the dark at room temperature with a cell concentration of 2x10⁷ cells/ml. 10 μl of mouse- anti-human CD8/PB (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flowcytometer.

In the above described example the Fluorescein-linker is as shown in figure 9 but the linker molecule can be any linker molecule as described in patent application WO

2007/015168 A2 (Lohse (2007)) or alternatively chemical biotinylated fluorochrom can be used instead of Fluorescein-linker molecules. The MHC complexes described in this example is a MHC I molecule composed of HLA-A^*0201 heavy chain, beta2microglobulin and NLVPMVATV (SEQ ID NO 9672) peptide but can in principle be any MHC complex or MHC like molecule as described elsewhere herein.

Example 10

This is an example of how to make MHC multimers consisting of a streptavidin multimerization domain with 3 biotinylated MHC complexs attached to the biotin binding pockets of streptavidin and how to use such trimer MHC complexs to detect specific T cells by direct detection of individual cells in a flow cytometry experiment by addition of a biotinylated flourophore molecule. In this example the fluorophore is Fluorescein linker molecules constructed as described elsewhere herein.

MHC complexs consisting of HLA-A^*0201 heavy chain, beta2microglobulin and peptide are generated as described elsewhere. MHC complexs are incubated with streptavidin in a molar ratio of 3:1 for Vz hour.

These trimer MHC multimers are then used to stain CMV specific T cells in a flow Cytometry experiment. 1x10⁶ purified HPBMC from a donor with T cells specific for HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) are incubated with 10 μl HLA- A^*0201 (peptide) multimer construct for 10 minutes in the dark at room temperature with a cell concentration of 2x10⁷ cells/ml. Then Fluorescein linker molecules (as described in Example 9) are added and incubation continued for 5 minutes. 10 μl mouse-anti- human CD8/PB antibody (clone DK25 from Dako) is added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by addition of 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. Cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flowcytometer.

In this example the Fluorescein-linker is as shown in figure 9 but the linker molecule can be any linker molecule as described in Lohse, Jesper, (2007), WO 2007/015168 A2 or alternative chemically biotinylated fluorochrome may be used. The MHC complexse described in this example is a MHC I molecule composed of HLA-A^*0201 heavy chain, beta2microglobulin and NLVPMVATV (SEQ ID NO 9672) peptide but can in principle be any MHC complex or MHC like molecule as described elsewhere herein.

Example 11

This is an example of how to make MHC multimers where the multimerization domain is dextran and the MHC complexs are chemically conjugated to the dextran multimerization domain. MHC complexs consisting of HLA-A^*0201 heavy chain, beta2microglobulin and NLVPMVATV (SEQ ID NO 9672) peptide or the negative control peptide GLAGDVSAV (SEQ ID NO 9671 ) are generated as described elsewhere herein. Dextran with a molecular weight of 270 kDa is activated with divinylsulfone. Activated Dextran is then incubated with MHC and RPE in a 0.05 M NaCHO₃ buffer; pH = 9.5 with a molar ratio between MHC and Dextran of 30-60 and a molar ratio between RPE and dextran of 3-7 :1 The mixture is placed in a water bath at 3O⁰C for 16 hours. Excess flourochrome, MHC and dextran are removed by FPLC using a sephacryl S-300 column. These MHC/RPE dextramers are then used to stain CMV specific T cells in a flow Cytometry experiment. Briefly, 1 x10⁶ purified HPBMC from a donor with T cells specific for HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) are incubated with 10 μl of each of the two HLA-A^*0201 (peptide)/RPE constructs described above for 10 minutes in the dark at room temperature with a cell concentration of 2x10⁷ cells/ml. 10 μl mouse-anti- human CD8/PB antibody (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The cells are then resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flow cytometer. Example 12

This is an example of how to make MHC multimers where the multimerization domain is dextran and MHC complexs are MHC I molecules chemically conjugated to dextran multimerization domain and the dextran multimerization domain also have fluorochrome chemically coupled.

Human beta2microglobulin is coupled to dextran as follows. Dextran with a molecular weight of 270 kDa is activated with divinylsulfone. Activated dextran is incubated with human beta2microglobulin and RPE in a 0.05 M NaCHO₃ buffer; pH = 9.5 with a molar ratio between beta2microglobulin and Dextran of 30-60 and a molar ratio between RPE and dextran of 3-7:1. The molar ratio of the final product is preferable 4-6 RPE and 15- 24 beta2microglobulin per dextran. The mixture is placed in a water bath at 3O⁰C for 16 hours. Excess flourochrome, beta2microglobulin and dextran are removed by FPLC using a sephacryl S-300 column. The beta2microglobulin-RPE-dextran construct is then refolded in vitro together with heavy chain and peptide using the following procedure. 200 ml refolding buffer (100 mM Tris, 400 mM L-arginin-HCL, 2 mM

NaEDTA, 0,5 mM oxidized Gluthathione, 5 mM reduced Glutathione, pH 8.0) supplied with protease inhibitors PMSF, Pepstatin A and Leupeptin (to a final concentration of 1 mM, 1 mg/l and 1 mg/l, respectively) is made and cooled to 1 O⁰C. 12 mg NLVPMVATV (SEQ ID NO 9672) peptide is dissolved in DMSO and added to the refolding buffer together with 20-30 mg beta2microglobulin-RPE-dex and 6 mg HLA-A^*0201 heavy chain. Incubation at 1 O⁰C for 4-8 hours, then 20-30 mg beta2microglobulin-RPE-dex and 6 mg HLA-A^*0201 heavy chain is added and incubation continued for 4-8 hours. Another 20-30 mg beta2microglobulin-RPE-dex and 6 mg HLA-A^*0201 heavy chain is added and incubation continued for 6-8 hours. The folding reaction is filtrated through a 0,2 μm filter to remove larger aggregates and then buffer exchanged into a buffer containing 20 mM Tris-HCI, 50 nM NaCI; pH = 8.0 followed by concentration to 1 -2 ml sample. Dextran-RPE-MHC complexs are then separated from excess heavy chain and peptide by size exclusion chromatography using a sephacryl S-300, S-400 or sephacryl S-500 column. These MHC/RPE dextramers may be used to stain CMV specific T cells in a flow

Cytometry experiment. Briefly, 1 x10⁶ purified HPBMC from a donor with T cells specific for HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) are incubated with 10 μl of each of the two HLA-A^*0201 (peptide)/RPE constructs described above for 10 minutes in the dark at room temperature with a cell concentration of 2x10⁷ cells/ml. 10 μl of mouse- anti-human CD8/PB antibody (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The cells are then resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flowcytometer.

Example 13

The preparation of a Pentamer MHC multimer is described in e.g. (United States Patent application 20040209295). Briefly, the following steps lead to a fluorescent Pentamer MHC multimer reagent:

The following is a detailed example for cloning, expressing, and purifying a pentameric class I MHC multimer, which comprises a chimeric fusion of .beta.2m with COMP. The chimeric .beta.2m-COMP protein is expressed in insoluble inclusion bodies in E. coli and subsequently assembled as pentameric .beta.2m-COMP in vitro. The pentameric class I MHC peptide multimer is then formed in a second refolding reaction by combining .beta.2m-COMP pentamers and the human MHC class I .alpha, molecule known as HLA-A^*0201 , in the presence of an appropriate synthetic binding peptide representing the T cell antigen. In this example, a well characterized antigen derived from Epstein-Barr virus BMLF1 protein, GLCTLVAML (SEQ ID NO 9675), is used. The resultant complex is labelled with a fluorescent entity and used as a staining reagent for detecting antigen-specific T cells from a mixed lymphocyte population, in a flow cytometry application.

The strategy involves the sequential cloning into pET-24c vector of .beta.2m, yielding a construct referred to as pETBMCOI , followed by the insertion of the oligomerisation domain of cartilage oligomeric matrix protein (COMP) with a biotin acceptor sequence (BP) for site-specific biotinylation with the biotin-protein ligase BirA, yielding a construct referred to as pETBMC02. Thirdly a polyglycine linker is cloned in between .beta.2m and COMP, yielding a construct referred to as pETBMC03, and finally, a serine-residue is removed by site-directed mutagenesis, which serine residue precedes the polyglycine linker, to give the final .beta.2m-COMP/pET-24c construct, referred to as pETBMC04 (see also FIG. 3). Removal of the serine residue is carried out to avoid steric hindrance when the .beta.2m molecule is associated with the MHC class I chain protein. -.

The extracellular portion of .beta.2m comprises of 99 amino acids (equivalent to Ile1 - Met99 of the mature protein) encoded by 74 bp-370 bp of the DNA sequence. This region of the .beta.2m sequence is amplified from a normal human lymphocyte cDNA library, by polymerase chain reaction (PCR)

beta.2m PCR product is purified from the above reaction mix using a QIAquick.RTM. PCR purification kit according to the manufacturer's instructions (Qiagen). 200 ng of purified PCR product and 1 .mu.g pET-24c vector (Novagen) are each digested with BamH I (10 U) and Nde I (10 U) restriction enzymes (New England Biolabs, NEB) for 4 h at 37.degree. C, in accordance with the manufacturer's instructions, and purified.

The gel-purified insert and vector DNA are ligated at a 1 :3 molar ratio (vectorinsert, 50 ng: 7.5 ng) using T4 DNA ligase (5 U; Bioline), in T4 DNA ligase buffer (as supplied) for 16 hrs at 16.degree. C.

The ligation mixtures and appropriate controls are subsequently transformed into XL1 - Blue strain competent E. coli cells, according to the manufacturer's instructions (Stratagene). Successful transformants are selected by plating the cells on Luria- Bertani (LB) agar plates containing 30 .mu.g/ml kanamycin, and incubating overnight at 37.degree. C.

A selection of single colonies from the bacterial transformation plates are screened by PCR with T7 promoter (1 .mu.M) and T7 terminator (1 .mu.M) primers (Sigma Genosys, see Appendix I for primer sequences), which are complementary to regions of the pET vector flanking the cloning site. Amplification is carried out using Taq DNA polymerase (1 U, Bioline) in Taq reaction buffer (as supplied), supplemented with 2 mM MgSO.sub.4 and 0.2 mM dNTPs, using 25 thermal-cycling reactions as detailed above. Successful transformants generated a DNA fragment of approximately 500 bp, ascertained by 1 .5% agarose gel electrophoresis.

Bacterial transformants that generated the correct size of PCR products are inoculated into 6 ml of sterile LB-kanamycin medium and incubated overnight at 37. degree. C. with 200 rpm shaking. pETBMCOI plasmid DNA is recovered from the bacterial cultures using a QIAprep.RTM. Spin Mini-prep kit according to the manufacturer's instructions (Qiagen). The presence of the .beta.2m fragment in these plasmids is further verified by automated DNA sequencing.

The sequence of the oligomerisation domain of COMP is obtained from the Genbank database (accession #1705995) and a region encoding the coiled-coil domain (amino acids 21 -85) is selected based on self-association experiments of COMP (Efinov et al., FEBS Letters 341 :54-58 (1994)). A biotin acceptor sequence ^'BP\ SLNDIFEAQKIEWHE is incorporated at the C terminus and an additional 14 amino acid linker, PQPQPKPQPKPEPET is included to provide a physical separation between the COMP oligomerising domain and BP.

The whole region is synthesized using the overlapping complementary oligonucleotides, and purified COMP-BP and 1 .mu.g pETBMCOI vector are digested for 4 hrs at 37. degree. C. using Hind III (10 U) and Xho I (10 U) restriction enzymes (NEB), as described in Section 1.1. The digestion products are purified, ligated, transformed and PCR screened as in Section 1 .1. Plasmids positive from the screen are purified and sequenced as described in Section 1 .1.

The poly-glycine linker is synthesized by annealing overlapping oligonucleotides. Since the nucleotide sequence of the polyGlycine linker only incorporates the 5' overhang of the cut BamH I restriction site, and the 3' overhang of the cut Hind III nucleotide recognition motifs, there is no need to digest the annealed product to produce the complementary single-stranded overhangs suitable for subsequent ligation. The oligonucleotides are phosphorylated and annealed as described in Section 1 .2.

pETBMC02 is digested with BamH I (10 U) and Hind III (10 U) . Ligation of the annealed poly-glycine linker into pETBMC02 was as described previously (Section 1 .1 ), assuming 96 fmoles of annealed oligonucleotide/.mu.l. The transformation and PCR-screening reactions are as described in Section 1 .1 , but in addition, the presence of an inserted linker is verified by a restriction enzyme digestion of the PCR screen product to ascertain the presence or absence of a Sal I restriction site. Successful transformants are not susceptible to Sal I digestion, given the removal of the site from the plasmid vector backbone. Purification of pETBMC03 and automated sequencing is as described in Section 1.1 .

Analysis of X-ray crystallography models of MHC class I molecules reveal that the C -.

terminus of .beta.2m closely abuts the .alpha.3 domain of the .alpha, chain. It is therefore desirable to achieve maximum flexibility at the start of the poly-glycine linker.

The extracellular portion of HLA-A^*0201 .alpha, chain (EMBL M84379) comprises of 276 amino acids (equivalent to GIyI -Pro276 of the mature protein) encoded by bases 73-900 of the messenger RNA sequence. In the following HLA-A^*0201 is used interchangeably with A^*0201. This region of the A^*0201 sequence is amplified from a normal human lymphocyte cDNA library by PCR, using suitable primers,which incorporated Ncol and BamHI restriction sites respectively. The procedure for cloning the A^*0201 insert into Nco l/BamH l-digested pET-1 ^"I d vector (Novagen) is essentially as described for .beta.2m in Section 1.1.

An identical procedure is carried out to produce either .beta.2m-COMP or A^*0201 .alpha, chain proteins. Plasmid DNA is transformed into an E. coli expression host strain in preparation for a large scale bacterial prep. Protein is produced as insoluble inclusion bodies within the bacterial cells, and is recovered by sonication. Purified inclusion bodies are solubilised in denaturing buffer and stored at -8O.degree. C. until required.

Purified plasmid DNA is transformed into the BL21 (DE3)pLysS E. coli strain, which carries a chromosomal copy of the T7 RNA polymerase required to drive protein expression from pET-based constructs. Transformations into BL21 (DE3)pLysS competent cells (Stratagene) are carried out with appropriate controls.

A single bacterial transformant colony is innoculated into 60 ml sterile LB medium, containing appropriate antibiotics for selection, and left to stand overnight in a warm room (.about.24.degree. C.) The resulting overnight culture is added to 6 litres of LB and grown at 37. degree. C. with shaking (.about.240 rpm), up to mid-log phase (OD.sub.600=0.3-0.4). Protein expression is induced at this stage by addition of 1.0 ml of 1 M IPTG to each flask. The cultures are left for a further 4 h at 37. degree. C. with shaking, after which the cells are harvested by centrifugation and the supernatant discarded.

The bacterial cell pellet is resuspended in ice-cold balanced salt solution and sonicated (XL series sonicator; Misonix Inc., USA) in a small glass beaker on ice in order to lyse the cells and release the protein inclusion bodies. Once the cells are completely lysed the inclusion bodies are spun down in 50 ml polycarbonate Oak Ridge centrifuge tubes in a Beckman high-speed centrifuge (J2 series) at 15,000 rpm for 10 min. The inclusion bodies are then washed three times in chilled Triton. RTM. wash This is followed by a final wash in detergent-free wash buffer.

The resultant purified protein preparation is solubilised in 20-50 ml of 8 M urea buffer, containing 50 mM MES, pH 6.5, 0.1 mM EDTA and 1 mM DTT, and left on an end- over-end rotator overnight at 4. degree. C. Insoluble particles are removed by centrifugation and the protein yield is determined using Bradford's protein assay reagent (Bio-Rad Laboratories) and by comparison with known standards. Urea- solubilised protein is dispensed in 10 mg aliquots and stored at -8O.degree. C. for future use.

Assembly of .beta.2m-COMP from the urea-solubilised inclusion bodies is performed by diluting the protein into 20 mM CAPS buffer, pH 1 1.0, containing 0.2 M sodium chloride and 1 mM EDTA, to give a final protein concentration of 1.5 mg/ml. The protein is oxidised at room temperature by addition of oxidised and reduced glutathione to final concentrations of 20 mM and 2 mM, respectively. Following an overnight incubation, disulphide bond formation is analysed by non-reducing SDS-PAGE on 10% bis-tricine gels (Invitrogen).

The protein mixture is subsequently buffer exchanged into 20 mM Tris, pH 8.0, 50 mM sodium chloride fS200 buffer^"), and concentrated to a final volume of 4.5 ml, in preparation for enzymatic biotinylation with BirA (Affinity, Denver, Colo.). 0.5 ml of l O.times. BirA reaction buffer (as supplied) is added, and recombinant BirA enzyme at 10 .mu.M final concentration, supplemented with 10 mM ATP, pH 7.0. A selection of protease inhibitors is also used to preserve the proteins: 0.2 mM PMSF, 2 .mu.g/ml pepstatin and 2 .mu.g/ml leupeptin. The reaction is left for 4 hours at room temperature.

Biotinylated .beta.2m-COMP is purified by size exclusion chromatography (SEC) on a Superdex.RTM.200 HR 26/60 column (Amersham Biosciences), running S200 buffer. 500 ml of refolding buffer is prepared as follows: 100 mM Tris, pH 8.0, 400 mM Larginine hydrochloride, 2 mM EDTA, 5 mM reduced glutathione and 0.5 mM oxidised glutathione, dissolved in deionised water and left stirring at 4. degree. C. 15 mg of lyophilised synthetic peptide GLCTLVAML (SEQ ID NO 9675) is dissolved in 0.5 ml dimethylsulfoxide and added to the refolding buffer whilst stirring. 50 mg of biotinylated pentameric .beta.2m-COMP and 30 mg of A^*0201 .alpha, chain is added sequentially, injected through a 23gauge hypodermic needle directly into the vigorously-stirred buffer, to ensure adequate dispersion. The refolding mixture is then left stirring gently for 16 hours at 4. degree. C.

The protein refolding mixture is subsequently concentrated from 500 ml to 20 ml using a MiniKros hollow fibre ultrafiltration cartridge (Spectrum Labs, Rancho Dominguez, Calif.) with a 30 kD molecular weight cutoff. Further concentration of the complex from 20 ml to 5 ml is carried out in Centricon Plus-20 centrifugal concentrators (30 kD molecular weight cut-off) according to the manufacturers instructions, followed by buffer exchange into S200 buffer using disposable PD10 desalting columns (Amersham Biosciences), according to the manufacturer's instructions. Final volume is 7.5 ml. The concentrated protein refold mixture is first purified by SEC on a Superdex.RTM. 200 HR 26/60 gel filtration chromatography column, as in Section 4.2. Fractions containing protein complexes in the region of 310 kD is collected.

Fractions collected from SEC are pooled and subjected to further purification by anion exchange chromatography on a MonoQ.RTM. HR 5/5 column (Amersham Biosciences), running a salt gradient from 0-0.5 M sodium chloride in 20 mM Tris over 15 column volumes. The dominant peak is collected. Protein recovery is determined using the Bradford assay.

Since each streptavidin molecule is able to bind up to 4 biotin entities, final labelling with phycoerythrin (PE)-conjugated streptavidin is carried out in a molar ratio of 1 :0.8, streptavidin to biotinylated pentamer complex respectively, taking into account the initial biotinylation efficiency measurement made for .beta.2m-COMP in Section 4.2. The total required amount of pentamer complex is subdivided (e.g. into 5 equal amounts) and titrated successively into streptavidin-PE. The concentration of A^*0201 pentamer-streptavidin complex is adjusted to 1 mg/ml with phosphate buffered saline (PBS), supplemented with 0.01% azide and 1% BSA. --

This resultant fluorescent Pentamer MHC multimer reagent is stored at 4.degree until use. This reagent may be used for detection of antigen specific T cells by flow cytometry , IHC or other procedures described herein usefull for detection of specific T cells using MHC multimers.

Pentamer MHC multimers are used in the following interchangeably with Pentamers or pentamer complexes.

Example 14 This is an example of how the directed approach described elsewhere herein for selection of antigenic peptides (as described elsewhere herein) is applied to an antigenic protein with known protein sequence, the cancer protein BcIX(L) encoded by the human genome. The purpose is to predict BcIX(L) peptide sequences that binds to MHC class 1 molecules for use in construction of MHC'mers designed to be used for analytical, diagnostic, prognostic, therapeutic and vaccine purposes, through the interaction of the MHC'mers with human BcIX(L) specific T-cells. Prediction is carried out using the known preferences of the 24 HLA class 1 alleles included in the http://www.cbs.dtu.dk/services/NetMHC/ database (figure 10).

The result of the prediction software is used to find all strong and weak 8-, 9-, 10- and 1 1 -mer peptide binders of the 24 HLA class 1 alleles. The result can be seen in Table 7. The MHC class 1 alleles for whom no binders are predicted are omitted from the list. The listed peptides are ranked according to decreased binding affinity for the individual MHC alleles. Strong binders are defined as binders with an affinity value of less than 50 nM and weak binders with a value of less than 50OnM. Only peptides defined as weak or strong binders are shown.

Example 15 Prediction of MHC class 2 peptide binders for human cancer protein BcIX(L) using directed approach

This is an example of how the directed approach described elsewhere herein for selection of antigenic peptides (as described elsewhere herein) is applied to an antigenic protein with known protein sequence, the cancer protein BcIX(L) encoded by the human genome. The purpose is to predict BcIX(L) peptide sequences that binds to MHC class 2 molecules for use in construction of MHC'mers designed to be used for CJ c.

analytical, diagnostic, prognostic, therapeutic and vaccine purposes, through the interaction of the MHC'mers with human BcIX(L) specific T-cells. Prediction is carried out using the known preferences of the 14 HLA class 2 alleles included in the http://www.cbs.dtu.dk/services/NetMHCII/ database (figure 10).

The result of the prediction software is used to find all strong and weak 15-mer peptide binders of the 14 HLA class 2 alleles. It also finds the important central nonamer core peptide sequence of each binding peptide. The result can be seen in Table 8. The MHC class 2 alleles for whom no binders are predicted are omitted from the list. The listed peptides are ranked according to decreased binding affinity for the individual MHC alleles. Strong binders are defined as binders with an affinity value of less than 50 nM and weak binders with a value of less than 50OnM. Only peptides defined as weak or strong binders are shown.

Example 16. Test of predicted BcIX(L) 10-mer binding peptide functionality in ELISPOT

In example 14 the best binding BcIX(L) 10-mer peptide for HLA-A^*0201 was identified to be YLNDHLEPWI (SEQ ID NO 9669). This peptide has then been tested in ELISPOT to see if it were able to detect the presence Bcl-X(L)-specific, CD8 positive T cells in PBL (Peripheral Blood Lymphocytes) from a breast cancer patient. PBL from a breast cancer patient was analyzed by ELISPOT ex vivo either with or without the BcI-X(L)173-182 peptide

(YLNDHLEPWI (SEQ ID NO 9669)), 106 PBL/well in doublets. The number of spots was counted using the lmmunospot Series 2.0 Analyzer (CTL Analysers). The result is given as number of spots above the pictures of the result as shown in Figure 1 1 and it clearly shows the presence of BcIX(L) specific T-cells and thereby the functionality of the peptide as compared to the absence of added peptide.

This example is from Cancer Immunol lmmunother Apr;56(4)527-33.

Example 17. Test of predicted BcIX(L) 10-mer binding peptide functionality in

Flow cytometry

In example 14 the best binding BcIX(L) 10-mer peptide for HLA-A^*0201 was identified to be YLNDHLEPWI (SEQ ID NO 9669). In the present example the functionality of the peptide is shown in a flow cytometric analysis of PBL from the patient was analyzed ex .. o

vivo by Flow cytometry to identify BcI-X(L)173-182 specific CD8 T cells using the dextramer complex HI_A-A2/Bcl-X(L)173-182-APC, 7-AAD-PerCP, CD3-FITC, and CD8-APC-Cy7. The dextramer complex HLA-A2/HIV-1 pol476-484-APC was used as negative control. The result (figure 12) clearly demonstrate that a MHC Dextramer HI_A-A^*0201/YI_NDHI_EPWI (SEQ ID NO 9669) complex detects BcIX(L) antigen specific CD-8 cells in the patient sample at a level of 0.03% as compared with the negative control using HIV specific MHC Dextramer. This example is from Cancer Immunol lmmunother Apr;56(4)527-33.

Example 18. Use of BcIX(L) specific MHC Dextramer for sorting of antigen specific CD8 T cells from patient sample

The antigen specific CD8 positive T-cells of example 17 were sorted out during the flow cytometric analysis using the MHC Dextramer HLA-A^*0201/YLNDHLEPWI (SEQ ID NO 9669). The detectable population of dextramer positive CD8 T cells was sorted as single cells into 96 well plates using the following protocol:

Small lymphocytes were gated by forward and side scatter profile, before cloning according to CD8/MHC-multimer double staining. CD8/MHC-multimer double-positive cells were sorted as single cells into 96 well plates (Nunc) already containing 10⁵ cloning mix cells/well. The cloning mix was prepared containing 10⁶ irradiated (20 Gy) lymphocytes from three healthy donors per ml in X-vivo with 5% heat-inactivated human serum, 25 mM HEPES buffer (GibcoBRL), 1 μg/ml phytohemagglutinin (PHA) (Peprotech) and 120 U/ml IL-2. The cloning mix was incubated for two hours at 37^/5 %CO₂, prior to cloning. After cloning, the plates were incubated at 37°C/5 %CO₂. Every 3 - 4 days 50 μl fresh media were added containing IL-2 to a final concentration of 120U/ml. Following 10 - 14 days of incubation, growing clones were further expanded using cloning mix cells. Consequently, each of the growing clones were transferred (split) into two or three wells (depending on the number of growing cells) of a new 96 well plate containing 5 x 10⁴ cloning mix cells/well. Clones that were not growing at this time were incubated for another week with IL-2, and then expanded. Subsequently, the specificity of the growing clones was tested in a ⁵¹Cr-release assay or by FACS.

Out of twenty-isolated dextramer positive CD8 T cells, ten were able to be expanded into T-cell clones.

This example is from Cancer Immunol lmmunother Apr;56(4)527-33. Example 19. Demonstration of specific cytolytic activity of isolated BcIX(L) specific CD8 T-cells

The ten expanded T cell clones isolated by Flow sorting as shown in example 18 were tested for their specificity by analysis in a standard 51 -Cr release assay. For this purpose, T2 cells loaded with either BcI-X(L)173-182 peptide or an irrelevant peptide (BA4697-105, GLQHWVPEL; (SEQ ID NO 9670)) were used as target cells. Five CD8 T-cell clones (Clone 8, 9, 10, 1 1 , and 12) effectively lysed T2 cells pulsed with BcI- X(L)173-182 without killing of T2 cells pulsed with an irrelevant peptide (Figure 13). One of these BcIX(L)173-182 specific CD8 T-cell clones [Clone 9] were expanded for further analyses. The remaining five expanded clones (Clone 7, 13, 15, 17, and 18) did not show specific lysis against T2 cells pulsed with BcI-X(L)173-182 peptide. This example is from Cancer Immunol lmmunother Apr;56(4)527-33.

Example 20. Demonstration of the cytotoxic capacity of a BcIX(L)173-182 specific CD8 T cell clone isolated by flow aided sorting of antigen (HLA- A*0201 /YLNDHLEPWI (SEQ ID NO 9669)) specific T cells.

The BcI-X(L)173-182 specific clone 9 from example 19 was expanded for additional 2 weeks before the cytotoxic potential was examined further in 51 Cr-release assays. Two assays were performed a Cell lysis of T2 cells pulsed with BcI-X(L) 173-182 peptide or an irrelevant peptide (BA4697-105, GLQHWVPEL (SEQ ID NO 9670)) in three E:T ratios, b Cell lysis of T2 cells pulsed with different concentrations of BcI-X(L)173-182 peptide at the E:T ratio 1 :1 The result is given in figure 14. As can be seen the presence of the specific peptide is necessary to get killing of the target cell and the effect of the peptide is significant even at low concentrations. This example is from Cancer Immunol lmmunother Apr;56(4)527-33.

Example 21. Synthesis of a comprehensive library of antigenic peptides of variable size derived from a full-length antigen sequence.

In this example it is described how virtually all of the possible 8'- to 2O'-mer peptide epitopes of an antigen may be synthetically prepared by modification of the standard Fmoc peptide synthesis protocol.

N-α-amino acids are incorporated into a peptide of the desired sequence with one end of the sequence remaining attached to a solid support matrix. All soluble reagents can be removed from the peptide-solid support matrix by filtration and washed away at the end of each coupling step. After each of the coupling steps, and after the removal of -.

reagents, a fraction of the generated peptides are removed and recovered from the polymeric support by cleavage of the cleavable linker that links the growing peptide to solid support.

The solid support can be a synthetic polymer that bears reactive groups such as -OH. These groups are made so that they can react easily with the carboxyl group of an N-α- protected amino acid, thereby covalently binding it to the polymer. The amino protecting group can then be removed and a second N-α-protected amino acid can be coupled to the attached amino acid. These steps are repeated until the desired sequence is obtained. At the end of the synthesis, a different reagent is applied to cleave the bond between the C-terminal amino acid and the polymer support; the peptide then goes into solution and can be obtained from the solution.

Initially, the first Fmoc amino acid (starting at the C-terminal end of the antigen sequence) is coupled to a precursor molecule on an insoluble support resin via an acid labile linker. Deprotection of Fmoc is accomplished by treatment of the amino acid with a base, usually piperidine. Before coupling the next amino acid, a fraction of the synthesized peptide (for example 0.1 %) is detached from the solid support, and recovered. Then additional beads carrying only the precursor molecule including the linker (for example corresponding to 0.1% of the total amount of solid support in the reaction) is added. Then the next Fmoc amino acid is coupled utilizing a pre-activated species or in situ activation.

This cycle of amino acid coupling, removal of reagents, detachment of a small fraction of synthesized peptide and recovery of these, and activation of the immobilized peptide to prepare for the next round of coupling, goes on until the entire antigen sequence has been processed.

The recovered peptides thus represent different fragments of the antigen, with varying lengths. The peptide pool thus contains most or all of the possible peptide epitopes of the antigen, and may be used in the preparation of MHC multimers as a pool.

The entire process, including the detachment of a fraction of the peptides after each round of coupling, follows standard Fmoc peptide synthesis protocols, and involves weak acids such as TFA or TMSBr, typical scavengers such as thiol compounds, phenol and water, and involves standard protecting groups. -.

Example 22

This is an example of how MHC multimers may be used for detection of Cytomegalovirus (CMV) specific T cells in blood samples from humans infected with CMV. In this example the MHC multimer used are MHC complexes coupled to fluorophor- labelled dextran (Dextramers). The dextramers are used for direct detection of TCR in flow cytometry. The antigen origin is CMV, thus, immune monitoring of CMV. MHC multimers carrying CMV specific peptides is in this example used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Purified MHC-peptide complexes consisting of HLA-A^*0201 heavy chain, human beta2microglobulin and peptide derived from a region in CMV internal matrix protein pp65 or a negative control peptide are generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated MHC-peptide complexes are then coupled to a 270 kDa dextran multimerization domain labelled with APC by interaction with streptavidin (SA) on the dextran multimerization domain. The dextran- APC-SA multimerization domain is generated as described elsewhere herein. MHC- peptide complexes are added in an amount corresponding to a ratio of three MHC- peptide molecules per SA molecule and each molecule dextran contains 3.7 SA molecule and 8.95 molecules APC. The final concentration of dextran is 3.8x1 Oe-8 M. The following MHC(peptide)/APC dextran constructs are made:

1. APC-SA conjugated 270 kDa dextran coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide NLVPMVATV (SEQ ID NO 9672) derived from CMV pp65.

2. APC-SA conjugated 270 kDa dextran coupled with HLA-A^*0201 in complex with beta2microglobulin and the non-sense peptide GLAGDVSAV (SEQ ID NO 9671 ) The binding of the above described MHC(peptide)/APC dextran is used to determine the presence of CMV pp65 specific T cells in the blood from CMV infected individuals by flow cytometry following a standard flow cytometry protocol. Blood from a patient with CMV infection is isolated and 100 ul of this blood is incubated with 10 μl of of the MHC(peptide)/APC dextran constructs described above for 10 minutes in the dark at room temperature. 5 μl of each of each of the antibodies mouse- anti-human CD3/PB (clone UCHT1 from Dako), and mouse-anti-human CD8/PE (clone DK25 from Dako) are added and the incubation continues for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 300xg and the supernatant removed. The washing step is repeated twice. The washed cells are resuspended in 400-500 μl PBS + 1% BSA; pH=7.2 and analyzed on flowcytometer. The presence of cells labeled with anti-CD3/PB, anti-CD8/PE and the MHC(peptide)/APC dextran construct 1 described above and thereby the presence of CMV specific T cells indicate that the patient are infected with Cytomegalovirus. Blood analysed with MHC(peptide)/APC dextran construct 2 show no staining of CD3 and CD8 positive cells with this MHC(peptide)/APC dextran construct. The result is shown in figure 15

The sensitivity of the above described test may be enhanced by addition of labeled antibodies specific for activation markers expressed in or on the surface of the CMV specific T cells.

We conclude that the MHC(peptide)/APC dextran constructs can be used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Example 23

This is an example of how MHC multimers may be used for detection of Cytomegalovirus (CMV) specific T cells in blood samples from humans infected with CMV.

In this example the MHC multimer used are MHC complexes coupled to fluorophor- labelled multimerisation domain Streptavidin (SA), used for direct detection of TCR in flow cytometry. The antigen origin is CMV, thus, immune monitoring of CMV. MHC multimers carrying CMV specific peptides is in this example used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Purified MHC-peptide complexes consisting of HLA-A^*0201 heavy chain, human beta2microglobulin and peptide derived from a region in CMV internal matrix protein pp65 or a negative control peptide were generated by in vitro refolding, purified and .. o

biotinylated as described elsewhere herein. Biotinylated MHC-peptide complexes are then coupled SA labelled with APC. MHC-peptide complexes were added in an amount corresponding to a ratio of 5 MHC-peptide molecules per SA molecule. Then SA/APC carrying four MHC complexes were purified from free SA, free monomeric MHC complex, SA carrying three, two and one MHC complexes. The following SA- MHC(peptide)/APC tetramers are made:

3. APC-SA coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide NLVPMVATV (SEQ ID NO 9672) derived from CMV pp65. 4. APC-SA coupled with HLA-A^*0201 in complex with beta2microglobulin and the non-sense peptide GLAGDVSAV (SEQ ID NO 9671 )

The binding of the above described MHC(peptide)/APC dextran can be used to determine the presence of CMV pp65 specific T cells in the blood from Cytomegalovirus infected individuals by flow cytometry following a standard flow cytometry protocol.

Blood from a patient with CMV is isolated and 100 ul of this blood is incubated with either of the SA- MHC(peptide)/APC tetramers described above for 10 minutes in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human CD3/PB (clone UCHT1 from Dako) and mouse-anti-human CD8/PE (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The washing step is repeated. The washed cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on flowcytometer.

The presence of cells labeled with anti-CD3/PB, anti-CDSVPE and the SA- MHC(peptide)/APC tetramers 3 described above and thereby the presence of CMV specific T cells will indicate that the patient are infected with Cytomegalovirus. Blood analysed with SA- MHC(peptide)/APC tetramers 4 should show no staining of CD3 and CD8 positive cells with this SA- MHC(peptide)/APC tetramer.

The sensitivity of the above described test may be enhanced by addition of labeled antibodies specific for activation markers expressed in or on the surface of the CMV specific T cells. We conclude that the APC-SA coupled MHC(peptide) constructs may be used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Example 24

This is an example of how MHC multimers may be used for detection of

Cytomegalovirus (CMV) specific T cells in blood samples from humans infected with

CMV.

In this example the MHC multimer used are MHC complexes coupled to any fluorophor-labelled multimerisation as described elsewhere herein. The MHC multimers are used for direct detection of TCR in flow cytometry. The antigen origin is CMV, thus, immune monitoring of CMV.

MHC multimers carrying CMV specific peptides is in this example used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Purified MHC-peptide complexes consisting of HLA-A^*0201 heavy chain, human beta2microglobulin and peptide derived a region in CMV internal matrix protein pp65 or a negative control peptide were generated by in vitro refolding and purified or purified from antigen presenting cells. MHC-peptide complexes are then coupled to a multimerisation domain together with APC.

The following MHC(peptide)/APC multimers are made:

5. APC-multimerisation domain coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide NLVPMVATV (SEQ ID NO 9672) derived from CMV pp65.

6. APC-multimerisation domain coupled with HLA-A^*0201 in complex with beta2microglobulin and the non-sense peptide GLAGDVSAV (SEQ ID NO 9671 ).

The binding of the above described MHC(peptide)/APC multimers can be used to determine the presence of CMV pp65 specific T cells in the blood from CMV infected individuals by flow cytometry following a standard flow cytometry protocol. Blood from a patient with CMV infection is isolated and 100 ul of this blood is incubated with either of the MHC(peptide)/APC multimers described above for 10 minutes in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human CD3/PB (clone UCHT1 from Dako) and mouse-anti- human CD8/PE (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The washing step is repeated. The washed cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on flowcytometer.

The presence of cells labeled with anti-CD3/PB, anti-CD8/PE and the MHC(peptide)/APC multimers 5 described above and thereby the presence of CMV specific T cells will indicate that the patient are infected with Cytomegalovirus. Blood analysed with MHC(peptide)/APC multimer 6 should show no staining of CD3 and CD8 positive cells with this SA- MHC(peptide)/APC multimer.

We conclude that the APC-multimerisation domain coupled MHC(peptide) constructs may be used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Example 25

Purified MHC-peptide complexes consisting of HLA-A^*2402 heavy chain, human beta2microglobulin and peptide derived from a region in CMV internal matrix protein pp65 or a negative control peptide are generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated MHC-peptide complexes are then coupled to a 270 kDa dextran multimerization domain labelled with APC by interaction with streptavidin (SA) on the dextran multimerization domain. The dextran- APC-SA multimerization domain is generated as described elsewhere herein. MHC- peptide complexes are added in an amount corresponding to a ratio of three MHC- peptide molecules per SA molecule and each molecule dextran contains 3.7 SA molecule and 8.95 molecules APC. The final concentration of dextran is 3.8x1 Oe-8 M. The following MHC(peptide)/APC dextran constructs are made:

7. APC-SA conjugated 270 kDa dextran coupled with HLA-A^*2402 in complex with beta2microglobulin and the peptide QYDPVAALF (SEQ ID NO 9683) derived from CMV pp65.

8. APC-SA conjugated 270 kDa dextran coupled with HLA-A^*2402 in complex with beta2microglobulin and the peptide VYALPLKML (SEQ ID NO 9684) derived from CMV pp65.

9. APC-SA conjugated 270 kDa dextran coupled with HLA-A^*2402 in complex with beta2microglobulin and the non-sense peptide.

The binding of the above described MHC(peptide)/APC dextran is used to determine the presence of CMV pp65 specific T cells in the blood from CMV infected individuals by flow cytometry following a standard flow cytometry protocol. Blood from a patient with CMV infection is isolated and 100 ul of this blood is incubated with 10 μl of of the MHC(peptide)/APC dextran constructs described above for 10 minutes in the dark at room temperature. 5 μl of each of each of the antibodies mouse- anti-human CD3/PB (clone UCHT1 from Dako), and mouse-anti-human CD8/PE (clone DK25 from Dako) are added and the incubation continues for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 300xg and the supernatant removed. The washing step is repeated. The washed cells are resuspended in 400-500 μl PBS + 1 % BSA; pH=7.2 and analyzed on flowcytometer.

The presence of cells labeled with anti-CD3/PB, anti-CD8/PE and the MHC(peptide)/APC dextran constructs 7 or 8 described above and thereby the presence of CMV specific T cells indicate that the patient are infected with Cytomegalovirus. Blood analysed with MHC(peptide)/APC dextran construct 9 show no staining of CD3 and CD8 positive cells with this MHC(peptide)/APC dextran construct. The sensitivity of the above described test may be enhanced by addition of labeled antibodies specific for activation markers expressed in or on the surface of the CMV specific T cells.

Example 26

This is an example of how MHC multimers may be used for detection of

CMV.

Purified MHC-peptide complexes consisting of HLA-A^*2402 heavy chain, human beta2microglobulin and peptide derived from a region in CMV internal matrix protein pp65 or a negative control peptide were generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated MHC-peptide complexes are then coupled SA labelled with APC. MHC-peptide complexes were added in an amount corresponding to a ratio of 5 MHC-peptide molecules per SA molecule. Then SA/APC carrying four MHC complexes were purified from free SA, free monomeric MHC complex, SA carrying three, two and one MHC complexes. The following SA- MHC(peptide)/APC tetramers are made:

10. APC-SA coupled with HLA-A^*2402 in complex with beta2microglobulin and the peptide QYDPVAALF (SEQ ID NO 9683) derived from CMV pp65.

1 1. APC-SA coupled with HLA-A^*2402 in complex with beta2microglobulin and the peptide VYALPLKML (SEQ ID NO 9684) derived from CMV pp65. 12. APC-SA coupled with HLA-A^*2402 in complex with beta2microglobulin and the non-sense peptide.

The binding of the above described MHC(peptide)/APC dextran can be used to determine the presence of CMV pp65 specific T cells in the blood from

Cytomegalovirus infected individuals by flow cytometry following a Standard flow cytometry protocol.

Blood from a patient with CMV is isolated and 100 ul of this blood is incubated with either of the SA- MHC(peptide)/APC tetramers described above for 10 minutes in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human

CD3/PB (clone UCHT1 from Dako) and mouse-anti-human CD8/PE (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The washing step is repeated. The washed cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on flowcytometer.

The presence of cells labeled with anti-CD3/PB, anti-CD8/PE and either of the SA- MHC(peptide)/APC tetramers 10 or 1 1 described above and thereby the presence of CMV specific T cells will indicate that the patient are infected with Cytomegalovirus. Blood analysed with SA- MHC(peptide)/APC tetramers 12 should show no staining of CD3 and CD8 positive cells with this SA- MHC(peptide)/APC tetramer.

We conclude that the APC-SA coupled MHC(peptide) constructs may be used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Example 27

This is an example of how MHC multimers may be used for detection of Cytomegalovirus (CMV) specific T cells in blood samples from humans infected with CMV. In this example the MHC multimer used are MHC complexes coupled to any fluorophor-labelled multimerisation as described elsewhere herein. The MHC multimers are used for direct detection of TCR in flow cytometry. The antigen origin is CMV, thus, immune monitoring of CMV. MHC multimers carrying CMV specific peptides is in this example used to detect the presence of CMV specific T cells in the blood of patients infected with Cytomegalovirus.

Purified MHC-peptide complexes consisting of HLA-A^*2402 heavy chain, human beta2microglobulin and peptide derived a region in CMV internal matrix protein pp65 or a negative control peptide were generated by in vitro refolding and purified or purified from antigen presenting cells. MHC-peptide complexes are then coupled to a multimerisation domain together with APC.

The following MHC(peptide)/APC multimers are made:

13. APC-multimerisation domain coupled with HLA-A^*2402 in complex with beta2microglobulin and the peptide QYDPVAALF (SEQ ID NO 9683) derived from CMV pp65.

14. APC-multimerisation domain coupled with HLA-A^*2402 in complex with beta2microglobulin and the peptide VYALPLKML (SEQ ID NO 9684) derived from CMV pp65.

15. APC-multimerisation domain coupled with HLA-A^*2402 in complex with beta2microglobulin and the non-sense peptide.

The binding of the above described MHC(peptide)/APC multimers can be used to determine the presence of CMV pp65 specific T cells in the blood from CMV infected individuals by flow cytometry following a standard flow cytometry protocol. Blood from a patient with CMV infection is isolated and 100 ul of this blood is incubated with either of the MHC(peptide)/APC multimers described above for 10 minutes in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human

CD3/PB (clone UCHT1 from Dako) and mouse-anti- human CD8/PE (clone DK25 from Dako) are added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples are then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The washing step is repeated. The washed cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on flowcytometer.

The presence of cells labeled with anti-CD3/PB, anti-CD8/PE and either of the MHC(peptide)/APC multimers 13 or 14 described above and thereby the presence of CMV specific T cells will indicate that the patient are infected with Cytomegalovirus. Blood analysed with MHC(peptide)/APC multimer 15 should show no staining of CD3 and CD8 positive cells with this SA- MHC(peptide)/APC multimer.

Example 28

This example describes how to identify specific T cells in a blood sample with MHC multimers using flow cytometry analysis without lysis of red blood cells and without washing the cells after staining. MHC complexes in this example consisted of HLA- A^*0201 heavy chain, human beta2microglobulin and different peptides, and the MHC complexes were coupled to a 270 kDa dextran multimerization domain. Purified MHC-peptide complexes consisting of human heavy chain, human beta2microglobulin and peptide were generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated MHC-peptide complexes were then coupled to a 270 kDa dextran multimerization domain labelled with PE by interaction with streptavidin (SA) on the dextran multimerization domain. The SA-PE- dextran was made as described elsewhere herein. MHC-peptide complexes was added in an amount corresponding to a ratio of three MHC-peptide moleculess per SA molecule and each molecule dextran contained 6.1 SA molecule and 3.9 molecules PE. The final concentration of dextran was 3.8x1 Oe-8 M. The following constructs were made:

1. PE conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide VTEHDTLLY (SEQ ID NO 9676) derived from Human Cytomegalo Virus (HCMV). 2. PE conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide IVDCLTEMY (SEQ ID NO 9677) derived from ubiquitin specific peptidase 9 (USP9). 3. PE conjugated 270 kDa dextran coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide NLVPMVATV (SEQ ID NO 9672) derived from Human Cytomegalo Virus (HCMV).

4. PE conjugated 270 kDa dextran coupled with HLA-A^*0201 in complex with beta2microglobulin and the peptide ILKEPVHGV (SEQ ID NO 9673) derived from Human Immunodeficiency Virus (HIV).

5. PE/SA conjugated 270 kDa dextran coupled with HLA-B^*0207 in complex with beta2microglobulin and the peptide TPRVTGGGAM (SEQ ID NO 9678) derived from Human Cytomegalo Virus (HCMV).

6. PE conjugated 270 kDa dextran coupled with HLA- B^*0207 in complex with beta2microglobulin and the peptide RPHERNGFTVL (SEQ ID NO 9679) derived from Human Cytomegalo Virus (HCMV).

7. PE conjugated 270 kDa dextran coupled with HLA- B^*0207 in complex with beta2microglobulin and the peptide TPGPGVRYPL (SEQ ID NO 9680) derived from Human Immunodeficiency Virus (HIV).

These seven MHC multimer constructs were used for detection of specific T cells in flow cytometry analysis using a no-lyse no-wash procedure. Blood samples from three individual donors were analyzed. The donors had previously been screened for the presence of specific T cells using a general staining procedure including lysis and wash of the cell sample, and donor one turned out to be positive for HLA^*0201 in complex with the peptide NLVPMVATV (SEQ ID NO 9672), donor two were positive for HLA^*0101 in complex with the peptide VTEHDTLLY (SEQ ID NO 9676) and donor three were positive for HLA-B^*0207 in complex with the peptides TPRVTGGGAM (SEQ ID NO 9678) and RPHERNGFTVL (SEQ ID NO 9679). In this experiment blood from each donor were analyzed with the MHC multimer construct they were supposed to have specific T cells restricted for and with MHC multimers of same haplotype but carrying a negative control peptide. The negative control peptides were either derived from HIV or the self-protein USP 9. Self-protein here means a naturally occurring protein in normal cells of a human individual. Normal healthy donors not infected with HIV are not expected to have specific T cells recognizing HIV derived peptides or peptides derived from self-proteins in complex with any HLA molecule in an amount detectable with this analysis method. The blood were stained as follows:

100 μl EDTA stabilized blood were incubated with 5 μl MHC(peptide)/PE dextran for 5 minutes at room temperature. Anti-CD45/PB, anti-CD3/FITC and anti-CD8/APC antibody in an amount of 0.4-1 .2 μg/sample was added to each tube and the incubation continued for another 15 minutes. 850 μl PBS; pH = 7.2 was added and the sample analyzed on a CyAn ADP flowcytometry instrument with a speed of 150 μl/minute. A total of 20.000 CD8 positive cells were acquired. During analysis CD45/PB antibody was used to set a trigger discriminator to allow the flow cytometer to distinguish between red blood cells and stained white blood cells (see figure 21 A). Furthermore CD3/FITC antibody was used to select CD3 positive cells in a second gating strategy (see figure 21 B). Blood from donor one showed specific staining with HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) multimer (construct 3) while no staining of specific T cells was observed with the negative control HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673)) multimer (construct 4). Donor two showed specific staining with HLA-A^*0101 (VTEHDTLLY (SEQ ID NO 9676)) multimer (construct 1 ) and no staining was observed with the negative control HLA-A^*0101 (IVDCLTEMY (SEQ ID NO 9677)) multimer (construct 2). In blood from donor three a population of T cells were stained with HLA-

B^*0207(TPRVTGGGAM (SEQ ID NO 9678)) multimer (construct 5) and another population with HLA-B^*0207(RPHERNGFTVL (SEQ ID NO 9679)) multimer (construct 6) while no specific staining was observed with the negative control HLA- B^*0207(TPGPGVRYPL (SEQ ID NO 9680)) multimer (construct 7). The results are shown in figure 22.

We have shown that MHC multimers of three different haplotypes can be used to identify specific T cells in blood samples from three different donors using an approach without lysing red blood cells and without wash following staining with MHC multimer. This method is simple, fast and interfere as little as possible with cells in the blood sample.

Example 29

This example illustrates how MHC multimers together with counting beads was used for exact numeration of MHC-peptide specific T cells in a flow cytometry analysis whit no lyses of red blood cells and no washing steps during or after staining. Counting beads in this example was CytoCount™, Count Control Beads from Dako that are polystyrene Fluorospheres with a diameter of 5.2 μm. The MHC multimer consisted of HLA-A^*0101 heavy chain complexed with human beta2microgloblin and a peptide and the MHC-peptide complexes were coupled to a 270 kDa dextran multimerization domain labelled with PE. MHC multimers were generated as described elsewhere herein and the following two constructs were made:

1 ) PE conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide VTEHDTLLY (SEQ ID NO 9676) derived from

Human Cytomegalo Virus (HCMV).

2) PE conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide IVDCLTEMY (SEQ ID NO 9677) derived from ubiquitin specific peptidase 9 (USP9).

Construct 2 is a negative control for construct 1 in this example and both were used for detection of specific T cells by flow cytometry using a no-lyse no-wash procedure: 100 μl of EDTA stabilized blood from a donor positive for HLA^*0101 in complex with the peptide VTEHDLLY were incubated with 5 μl MHC multimer for 5 minutes at room temperature. Anti-CD45/CY, anti-CD3/PB and anti-CD8/APC antibody in an amount of 0.4-1.2 μg/sample was added and the incubation continued for another 15 minutes. 850 μl PBS; pH = 7.2 was added together with precise 50 μl CytoCount beads 1028 bead/μl and the sample analyzed on a CyAn ADP flowcytometry instrument with a speed of 150 μl/minute. A total of 20.000 CD8 positive cells were acquired. During analysis CD45/CY antibody was used to set a trigger discriminator to allow the flow cytometer to distinguish between red blood cells and stained white blood cells. A dot plot was made for each sample showing MHC multimer vs CD8 positive events (se figure 23 A and B). Based on the negative control a gate comprising events representing CD8 positive T cells specific for MHC multimer was defined. Similarly histogram plots for each sample was made showing FITC signal vs counts (figure 23 C and D). In these histograms the amount of beads in the analyzed sample were identified since the beads in contrast to the cells emit light in the FITC channel. In principle the beads could be visualized in any fluorochrome channel because they emit light in all channels but it was important to visualize the beads in a channel where there was no interfering signal from labelled cells. The concentration of T cells specific for HLA-A^*0101 (VTEHDTLLY (SEQ ID NO 9676)) multimer (construct 1 ) in the blood sample were determined using the counting beads as an internal standard. Events obtained from staining with the negative control MHC multimer, construct 2, were defined as background signals and subtracted from the result obtained from staining with construct 1.

Concentration of HLA-A^*0101 (VTEHDTLLY (SEQ ID NO 9676)) specific T cells in the blood sample =

((Count of MHC multimer÷ CD8+ positive cells, construct 1 x concentration of beads x dilution factor of beads) /counted beads))- ((Counted MHC multimer+ CD8+ cells, construct 2 x concentration of beads x dilution factor of beads) /counted beads) = 992,6 cells/ml

For details se figure 23.

This experiment demonstrated how CytoCount™ counting beads together with MHC multimers could be used to determine the exact concentration of MHC-peptide specific T cells in a blood sample using a no-lyse no-wash method.

Example 30

This example describes an analysis of specific T cells in blood using MHC multimers where MHC multimers together with antibodies are pre-mixed and embedded in a matrix material to retain and immobilize the reagents prior to use. In this example the matrix was composed of Trehalose and Fructose and the MHC complex consisted of HLA-A^*0101 heavy chain complexed with human beta2microglobulin and peptide. The MHC-peptide complexes were coupled to a 270 kDa dextran multimerization domain.

Purified MHC-peptide complexes consisting of heavy chain, human beta2microglobulin and peptide were generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated MHC(peptide) complexes were coupled to a 270 kDa dextran multimerization domain labelled with PE, thereby generating PE labelled MHC multimers. The following MHC multimer constructs were made:

1 ) PE conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with beta2microglobulin and the peptide VTEHDTLLY (SEQ ID NO 9676) derived from Human Cytomegalo Virus (HCMV).

2) PE conjugated 270 kDa dextran coupled with HLA-A^*0101 in complex with -.

beta2microglobulin and the negative control peptide IVDCLTEMY (SEQ ID NO 9677) derived from ubiquitin specific peptidase 9 (USP9).

Tubes with a matrix material to retain and immobilize the above described MHC multimer constructs together with antibodies relevant for later flow cytometer analysis was made. The matrix material was made to retain MHC multimer and antibody in the container when dry but release them into the sample medium when a sample comprising cells of interest was added to the tube. Experimentally, solutions of 20% Fructose in water and 20% Trehalose in water were made and mixed in a 1 :1 ratio. 15 μl of this mixture were transferred to two 5 ml Falcon tubes. A premix of antibodies were made consisting of 40 μl anti-CD8 Alexa700 labelled antibody in a concentration of 25 μg/ml + 40 μl anti-CD3 Pacific Blue labelled antibody in a concentration of 100 μg/ml + 160 μl anti-CD45 Cascade Yellow labelled antibody in a concentration of 200 μg/ml. 12 μl of this mixture were added to each Falcon tube together with 3 μl of either of the two MHC multimer constructs. 100 μl butylated hydroxytoluen (BHT) with a concentration of 99 mg/L were added. The mixtures were dried under vacuum a 2-8⁰C over night. 100 μl EDTA stabilized blood from a donor with T cells specific for HLA-A^*0101 complexed with the peptide VTEHDTLLY (SEQ ID NO 9676) were added to each of the two tubes. As a control experiment 6 μl of the antibody premix described above were transferred to two empty 5 ml Falcon tubes together with 3 μl of either of the MHC multimer constructs and 100 μl blood from the same donor. All four tubes were incubated for 15 minutes at room temperature. Then 900 μl PBS; pH = 7.2 was added and the sample analyzed on a CyAn ADP flowcytometer instrument. A total of 20.000 CD8 positive cells were acquired for each sample. During analysis

CD45/CY antibody was used to set a trigger discriminator to allow the flow cytometer to distinguish between red blood cells and stained white blood cells.

As expected and shown in figure 24 a population of CD8 positive and HLA-

A^*0101 (VTEHDTLLY (SEQ ID NO 9676)) multimer positive cells were observed in the two samples stained with construct 1. The amount of specific T cells detected in the matrix sample was comparable to the amount of specific T cells detected in the control sample without matrix material. No HLA-A^*0101 (IVDCLTEMY (SEQ ID NO 9677)) multimer specific CD8 positive cells were observed in the two samples stained with the negative control MHC multimer construct 2. -.

This experiment demonstrates that the MHC multimer constructs used in this experiment can be embedded in a sugar matrix and later used for analysis of specific T cells in a blood sample and that this method gives results comparable to results obtained from a no-lyse no-wash staining procedure.

Example 31

This example describes the generation and application of negative controls, where the MHC complex is HLA-A^*0201 loaded with either of the nonsense peptides GLAGDVSAV (SEQ ID NO 9671 ) or ALIAPVHAV (SEQ ID NO 9674) and these MHC complexes are coupled to a 270 kDa dextran multimerization domain. The nonsense peptides have an amino acid sequence different from the linear sequence of any peptide derived from any known naturally occurring protein. This was analyzed by a blast search. The amino acids at position 2 and 9 can serve as anchor residues when binding to HLA-A^*0201 molecules.

Purified MHC(peptide) molecules consisting of the allele HLA-A^*0201 , human beta2microglobulin and peptide was generated by in vitro refolding, purified and biotinylated as described elsewhere herin. Biotinylated HLA-A^*0201 (peptide) was mixed with APC-SA-conjugated 270 kDa dextran in an amount corresponding to a ratio of three biotinylated H LA- A^*0201 (peptide) molecules per SA molecule and incubated for 30 minutes in the dark at room temperature. The APC-SA-conjugated 270 kDa dextran contained 9 molecules APC and 3,7 molecules SA per dextran molecule. Following incubation the mixture was diluted into a buffer comprising 0,05M Tris/HCI, 15 nM NaN₃ and 1 % BSA to a final concentration of 3,8x10^'8 M dextran. By this procedure the following MHC multimer constructs were made:

1 ) A negative control construct comprising APC-SA-conjugated 270 kDa dextran and biotinylated HLA-A^*0201 in complex with beta2microglobulin and the nonsense peptide GLAGDVSAV (nonsense peptide 1 ; (SEQ ID NO 9671 )). 2) A negative control construct comprising APC-SA-conjugated 270 kDa dextran and biotinylated HLA-A^*0201 in complex with beta2microglobulin and the nonsense peptide ALIAPVHAV (nonsense peptide 2; (SEQ ID NO 9674)).

3) A construct comprising APC-SA-conjugated 270 kDa dextran and biotinylated HLA-A^*0201 in complex with beta2microglobulin and the peptide NLVPMVATV (SEQ ID NO 9672) derived from pp65 protein from human cytomegalovirus (HCMV).

4) A construct comprising APC-SA-conjugated 270 kDa dextran and biotinylated HLA-A^*0201 in complex with beta2microglobulin and the peptide GLCTLVAML (SEQ ID NO 9675) derived from BMLF-1 protein from Epstein Barr virus (EBV).

5) A construct comprising APC-SA-conjugated 270 kDa dextran and biotinylated HLA-A^*0201 in complex with beta2microglobulin and the peptide ILKEPVHGV (SEQ ID NO 9673) Reverse Transcriptase from Human Immunodeficiency Virus (HIV).

The binding of the HLA-A^*0201 (peptide)/APC dextran constructs to Human Peripheral Blood Mononuclear Cells (HPBMC) from various donors was analyzed by flow cytometry following a standard flow cytometry protocol. Briefly, HPBMC from the blood of 9 individual donors were isolated, by a standard protocol using Ficoll-Hypaque. 1 x10⁶ purified HPBMC at a concentration of 2x10⁷ cells/ml were incubated with 10 μl of one of the HLA-A^*0201 (peptide)/APC dextran constructs described above for 10 minutes in the dark at room temperature. 10 μl of each of the antibodies mouse-anti- human CD3/PE (clone UCHT1 from Dako) and mouse-anti-human CD8/PB (clone DK25 from Dako) were added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples were then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 200xg and the supernatant removed. The cells were then resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a CYAN ADP flowcytometer. Donor 1 -5 were known to have detectable T cells specific for HLA- A^*0201 (NLVPMVATV (SEQ ID NO 9672)) and no detectable T cells specific for HLA- A^*0201 (ILKEPVHGV (SEQ ID NO 9673)) while donor 6 were known not to have detectable specific T cells for either H LA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) nor HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673)). Lymphocytes from these 6 donors were stained with MHC multimer construct 1 , 2, 3, and 5. Donor 1 -5 showed positive staining with MHC multimer construct 3 as expected while no staining was observed with the either of the negative control MHC complex constructs 1 and 2 or with MHC complex construct 5. An example showing the staining patterns for donor 2 is shown in figure 19. No specific staining was observed of lymphocytes from donor 6 with either of the MHC multimer constructs. --OO

Donor 7-8 known to have detectable T cells specific for HI_A-A^*0201 (GLCTLVAML (SEQ ID NO 9675)) and no detectable T cells recognizing HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673)) and donor 9 having no detectable T cells specific for either HLA- A^*0201 (GLCTLVAML (SEQ ID NO 9675)) nor HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673)) were all stained with MHC multimer construct 1 , 2, 4, and 5. Donor 7 and 8 demonstrated efficient staining with MHC multimer construct 4 as expected while no staining was observed with the other MHC multimer constructs tested. No staining was observed of lymphocytes from donor 9 with either of the MHC multimer constructs tested. A summary of the results is shown in figure 20. In conclusion this experiment demonstrates that the negative MHC multimer constructs 1 and 2 did not stain any specific T cells in lymphocyte preparations from 10 different donors. Donors known to have specific T cells for either HLA-A^*0201 (GLCTLVAML (SEQ ID NO 9675)) or HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) also demonstrated positive staining with the corresponding MHC multimer constructs 3 and 4. None of the 10 donors were infected with HIV and as expected did not appear to have T cells specific for HLA-A^*0201 in complex with the HIV derived peptide ILKEPVHGV (SEQ ID NO 9673), and as expected none of these donors showed staining with MHC multimere construct 5. MHC multimer construct 1 and 2 are therefore suitable negative controls when using H LA-A^*0201 (peptide) multimers for detection of specific T cells in Flow Cytometry.

Example 32

This example describes the generation of a negative control, where the MHC complex is HLA-A^*0201 coupled to a 270 kDa dextran , and where the MHC is loaded with the peptide ILAKFLHWL that have pivaloyl coupled to Lysine at position 4. ILAKFLHWL is a peptide derived from telomerase and is known to bind HLA-A^*0201. Pivaloyl is a small molecule that confers high sterical hindrance. Because pivaloyl is placed at a central position in the peptide it is likely to inhibit or completely abrogate the interaction with a specific TCR, because TCR-recognition is normally directed to the middle of the peptide when bound in the peptide-binding cleft. In the following the pivaloyl-modified peptide will be designated ILAK^PFLHWL.

Purified HLA-A^*0201 (ILAK^pFLHWL) molecules consisting of the HLA-A^*0201 heavy chain, human beta2microglobulin and ILAK^PFLHWL peptide is generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated HLA- A^*0201 (ILAK^pFLHWL) molecules are mixed with flourochrome-SA-conjugated 270 kDa dextran molecules. The resulting HLA-A^*0201 (ILAK^pFLHWL)/flourochrome-carrying dextran molecules can be used as negative controls in e.g. flow cytometric analysis.

Example 33

This example describes the generation of a negative control, where the MHC complex is any MHC I or MHC Il molecule of human, mouse, rabbit, rat, swine, monkey or any other origin loaded with the peptide ILAKTLHWL and coupled to any multimerization domain labeled with fluorochrome, HRP or any other label. Purified MHC(ILAK^PFLHWL) complexes consisting of the heavy chain, human beta2microglobulin and ILAK^PFLHWL peptide is generated by in vitro refolding, purified and biotinylated as described elsewhere herein. Biotinylated MHC(ILAK^PFLHWL) complexes are mixed with labeled multimerization domain, thereby generating MHC(ILAK^PFLHWL) multimers. The MHC(ILAK^PFLHWL) multimers mayn be used as negative controls in e.g. flow cytometric analysis, IHC, ELISA or similar.

Example 34

This example describes how to verify that a MHC-complex is correctly folded by a sandwich-ELISA assay. W6/32 mouse-anti-HLA-ABC antibody (Dako M0736), that recognizes a conformational epitope on correctly folded MHC-complex , was used as coating-antibody. HRP-conjugated rabbit anti-β2m (Dako PO174) was used for visualization.

1 . Wells of a microtiter plate was pre-coated with W6/32 antibody (Dako M0736, 5 μg/ml in 0.1 M NaHCO₃, 1 mM MgCI₂, pH 9.8, 50 μl/well) following a standard ELISA procedure regarding washes and blocking ect.

2. After addition of 50 μl of 0.5M Tris-HCI, 0.1 M NaCI, 0.1 % Tween 20, 0.01% Bronidox, pH 7.2 to each well, 50 μl of a sample of purified folded MHC-complex (in a concentration of approx. 0.4 mg/ml) was added to two wells in to columns in the microtiter plate, diluted 2-fold down the column and incubated 2 hours at 4⁰C. Light chain β2m (0.15 mg/ml in 0.5M Tris-HCI, 0.1 M NaCI, 0.1% Tween 20, 0.01% Bronidox, pH 7.2) was used as a negative control and the cell-line KG-I a, -.

expressing HLA-A^*30, HLA-A^*31 and HLA-B^*35 heavy chains, was used as positive control (10⁶ cells/well).

3. After a Standard ELISA wash, 50 μl of the detecting antibody; HRP-conjugated rabbit anti-β2m (Dako PO174), diluted 1 :2500 in 1 % Skimmed Milk in 0.5M Tris- HCI, 0.1 M NaCI, 0.1% Tween 20, 0.01 % Bronidox, pH 7.2 was added to each well.

The plate wass incubated 1 hour at 4⁰C.

4. After a standard ELISA wash, 50 μl of an amplifying antibody; HRP-Dextran500- conjugated goat anti-rabbit (Dako DM0106), diluted 1 :2000 in 1 % Skimmed Milk in 0.5M Tris-HCI, 0.1 M NaCI, 0.1% Tween 20, 0.01% Bronidox, 1% mouse serum (Dako X0190) pH 7.2 was added. The plate was incubated 30 min. at 2O⁰C.

5. After a standard ELISA wash, 50 μl of Dako S1599 (TMB+Substrat Chromogen) was added to each well for visualization.

6. After 10 min. the visualization reaction was stopped with 50 μl 0,5M H₂SO₄/well.

7. The chromogenic intensity was measured at OD₄₅₀ and the result from the ELISA assay evaluated.

As shown in figure 16 the OD₄₅₀ values from wells with MHC complex was more than 6 times higher than OD₄₅₀ values from wells with the negative control β2m. This ELISA procedure can be used to verify the presence of correctly folded MHC-peptide complexes in a preparation of MHC complexes.

Example 35

This example describes how the quality of a MHC multimer can be tested. The MHC multimer is in this example a MHC-dextramer, and the test involves specific binding of the MHC-dextramer to TCRs immobilized on beads.

Recombinant TCRs (CMV3 TCRs; Soluble CMVpp65(NLVPMVATV (SEQ ID NO

9672))-specific TCR protein) specific for the MHC-peptide complex HLA-

A^*0201 (NLVPMVATV (SEQ ID NO 9672)), where the letters in parenthesis denote the peptide complexed to the MHC-allel HLA-A^*0201 , were obtained from Altor Biosciences. The TCRs were dimers linked together via an IgG framework. The purity of the TCRs were verified by SDS PAGE and was between 95-100% pure. The quality of the TCRs were verified by their ability to recognize the relevant MHC- dextramer and not irrelevant MHC dextramers in ELISA experiments (data not shown). -. o

Carboxylate-modified beads were coupled with dimeric TCR (CMV3 TCRs; Soluble CMVpp65(NLVPMVATV (SEQ ID NO 9672))-specific TCR protein), incubated with fluorescently labeled MHC-dextramers and the extend of cell staining analysed by flow cytometry, as follows:

Immobilization of TCR on carboxylate beads:

1 . 3x10⁹ Carboxylate-modified beads, Duke Scientific Corporation, XPR-1536, 4μm, lot:4394 were washed in 2x 500μl Wash buffer 1 (0,05% Tetronic 1307, 0,1 M MES- buffer (2-[N-morpholino]ethanesulfonic acid), pH 6,0), centrifuged 4 min at 15000 g, and the supernatant was discarded.

2. 125 μl EDAC/Sulfo-NHS (5OmM EDAC (1 -ethyl-3-(3- dimethylaminopropyl)carbodiimide), 5OmM Sulfo-NHS, in Wash buffer 1 ) was added to the beads, and the suspension incubated at room temperature for 20 min.

3. Beads were washed in 2x 250μl Wash buffer 1 and centrifuged 2 min at 15000 g, and the supernatant was discarded.

4. TCR was added in various concentrations from 0 μg to 20 μg, and incubated with slow shaking overnight at 4⁹C.

5. Beads were centrifuged 4 min at 15000 g, and the supernatant discarded.

6. Beads were washed in 2x 500μl Wash buffer 1 and centrifuged 4 min at 1500 g, and the supernatant was discarded.

7. 125μl 2OmM Glycin in Wash buffer 1 was added, and resuspended beads incubated for 1 hour at room temperature.

8. Beads were washed in 2x 500μl phosphate-buffered saline (PBS) pH 7.2, 0.5 % Tetronic 1307, and centrifuged 2 min at 15000 g, and the supernatant was discarded.

9. Beads were resuspended in 250μl PBS pH 7.2, 0,05% Tetronic 1307.

Bead concentration after resuspension was 1 ,2x10⁷ beads/μl. Beads coated with TCR were stored at 2-8⁹C until further use.

Flow cytometry analysis:

1. 20μl beads (1 ,2x10⁷ beads/μl) coated with 0-20μg TCRs, as described above were washed in 200μl Wash buffer 2 (5% FCS, PBS, pH 7.4).

2. Beads were centrifuged 3 min at 12000 g, and the supernatant was discarded, and beads resuspended in 50μl Wash buffer 2. 3. 10μl MHC-dextramers were added, and samples were incubated 15 min. at room temperature in the dark.

4. Samples were washed in 1 ml Wash buffer 2, centrifuged at 300 g for 5 min. The supernatant was discarded, and pellet resuspended in 0.4 ml PBS pH 7.4, and kept at 4°C in the dark until analysis on flow cytometer.

5. Samples were analysed by flow cytometry on a CyAn instrument.

The results are shown in figure 17. Beads coated with 2-20μg TCR all showed positive staining with the specific HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672))/RPE and not with an irrelevant HLA-A^*0201 (ILKEPVHGV (SEQ ID NO 9673))/RPE dextramer. It can be concluded that carboxylate beads coated with dimeric TCRs can be used to test the quality of the MHC-dextramers.

Example 36 This example describes how TCR-coated beads can be used as internal, positive controls when analysing suspensions of Human Peripheral Blood Mononuclear Cells (HPBMCs), whole blood samples or any other cell sample of interest. The MHC multimer employed in this example is a MHC-dextramer.

In this example TCR-coated carboxylated beads generated as described in example 35 were added to a sample comprising either HPBMCs or whole peripheral blood.

HPBMCs and TCR-beads were incubated with fluorescently labelled MHC- dextramers and the extent of cell staining analysed by flow cytometry according to this general staining procedure:

1. Transfer 1 -3 x 10⁶ lymphoid cells (PBMC or splenocytes) to a 12 x 75 mm polystyrene test tube. Other cells of interest can be used. Allocate only 2-5 x 10⁵ cells per tube when staining T-cell clones or cell lines due to the high frequency of antigen-specific T cells 2. Add 2 ml 0,01 mol/L PBS comprising 5% fetal calf serum and centrifuge at

30Ox g for 5 minutes. Remove supernatant and resuspend cells in remaining liquid.

3. Add 10 μl of MHC Dextramer and mix gently with a vortex mixer. Incubate in the dark at room temperature for 10 minutes. -- O

4. Add an optimally titrated amount of anti-CD8 antibody conjugated with a relevant flourochrome (e.g. Dako clone DK25 for human lymphocytes or clone YTS169.4/KT15 for mouse lymphocytes). Incubate in the dark at 2- 8°C for 20 min. 5. Add 2 ml of 0,01 mol/L PBS comprising 5% fetal calf serum and centrifuge at 30Ox g for 5 minutes.

6. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.4 ml PBS. Analyse on a flow cytometer or store at 2-8⁰C in the dark until analysis. Do not store longer than 2 hours before analysis.

Human peripheral whole blood and TCR-beads were incubated with fluorescently labelled MHC-dextramers and the extent of cell staining analysed by flow cytometry as follows:

1. Transfer 100 μl_ whole blood to a 12 x 75 mm polystyrene test tube.

2. Add 10 μl of MHC Dextramer and mix with a vortex mixer. Incubate in the dark at room temperature for 10 minutes.

3. Add an optimally titrated amount of anti-CD8 antibody (e.g. Dako clone DK25) conjugated with a relevant fluorochromes and mix well. Continue incubation at 2-8 ^QC in the dark for 20 minutes.

4. Add 2 ml_ EasyLyse™ working solution (Code No. S2364) and incubate for 10 minutes.

5. Centrifuge for 5 minutes at 300 x g and aspirate supernatant.

6. Add 2 ml_ 0.01 mol/L PBS and centrifuge for 5 minutes at 300 x g and aspirate supernatant.

7. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.4 ml_ PBS, and analyze on a flow cytometer or store at 2-8 ^QC in the dark until analysis. Do not store longer than 2 hours before analysis.

Figure 18 shows examples of TCR-beads added into whole blood or HPBMC samples.

In both experiments it is possible, by forward- vs. side-scatter measurements, to distinguish TCR-beads from cell populations in the sample. Region R1 is TCR- beads, and region R2 is lymphocyte cell population of interest in the analysis of MHC positive T cells.

The size and conditions of coating of beads might be optimized. The size of beads or labeling of beads (e.g. flourescent labeling) can be optimized to allow separation of cells of interest in the sample. In this example the forward- vs. side- scatter dot plot has been used for gating of cell populations of interest. Other parameters (e.g. fluorescence intensity) for cell populations of interest can be used.

Human peripheral whole blood and other cells (e.g. HPBMCs) can be stained with MHC Dextramers simultaneously with immuno-phenotyping of relevant antigens.

The staining procedure describes the use of labelled CD8 antibody together with MHC dextramers; additional antibodies for detection of other extracellular antigens can be added. Likewise, detection of intracellular antigens can be performed simultaneously with MHC-detection (for protocol, see IntraStain procedure, cat no. K231 1 , Dako. Additional washing step prior to IntraStain Reagent A is essential for good results using MHC Dextramers together with this IntraStain procedure).

Example 37

This is an example of measurement of antigen reactive T-CeIIs by IFN-γ capture in blood samples by ELISPOT.

This is an example of indirect detection of TCR, where individual cells are immobilized and measured by a chromogen assay.

The example provides a sensitive assay for the detection of T-cells reactive to an antigen by detecting a soluble factor whose secretion is induced by stimulation of the T-cell by the antigen.

A summary flow chart of the method is shown in figure 25. In brief, peripheral blood is diluted threefold in Dulbecco's phosphate buffered saline (DPBS), underlain with 15 ml of Ficoll (Pharmacia Ficoll-Paque #17-0840-02, Piscataway, NJ.) per 40 ml diluted blood in a 50 ml polypropylene centrifuge tube, and spun at 2000 RPM for 20 minutes in a Beckman CS-6R centrifuge (Beckman Inc., Palo Alto, Calif.). The buffy layer at the DPBS/Ficoll interface is removed, washed twice with DPBS and once with human tissue culture medium (hTCM: αMEM+5% heat inactivated human AB serum (Ultraserum, BioWhittaker, Walkersville, Md.), penicillin/streptomycin, 1 -glutamine) at low RCF to remove platelets. Sixty percent of the PBMCs are resuspended in freezing medium (10% dimethyl sulfoxide(Sigma Chenical Co., St. Louis, Mo.), 90% fetal bovine serum to a concentration of 5x10⁶ cells/ml, frozen in a programmable Cryo-Med (New Baltimore, Mich.) cell freezer, and stored under liquid nitrogen until needed.

The purified PBMCs are plated at 2x10⁵ cells/well at a volume of 0.1 ml in 96 well Costar cell culture plates. An equal volume of antigen at 10 μg/ml is added to triplicate or sextuplet sets of wells and the plate is incubated in a 37 °C., 5% CO₂ incubator. On day five, 10 μl/well of 100 U/ml stock recombinant IL-2 (Advanced Biotechnologies Inc., Columbia, Md.) is added to each well. On day 8, frozen PBMCs are thawed, washed in DPBS+0.5% bovine serum albumin (BSA) to remove DMSO, resuspended to a concentration of 4x10⁶ cells/ml in hTCM, and γ-irradiated (3,000 RADS). Fifty micro liters/well are dispensed along with 50 μl of the appropriate antigen at a stock concentration of 40 μl/ml to give a final antigen concentration of 10 μg/ml.

To prepare a capture plate, IFN-γ capture antibody (monoclonal mouse anti-human IFN-g, Endogen #M700A, Cambridge, Mass.) is diluted to 10 μg/ml in sterile 0.1 M Na(CO₃)₂ pH 8.2 buffer, aliquotted at 50 μl/well in flat bottomed 96 well sterile microtiter plates (Corning Costar Corp.), and incubated at 4^ for a minimum of 24 hours. Prior to use, excess antibody is removed and wells are washed twice with dPBS+1% Tween 20 (PBST). To block further nonspecific protein binding, plates are incubated with 250 μl/well of PBS+5% BSA at room temperature for 1 hour. After discarding the blocking solution, wells are washed once with PBST (0.1% Tween), followed by hTCM in preparation for the antigen stimulated cells.

On day 9 of the assay, twenty four hours after the second antigen stimulation, the stimulation plate is spun for 5 minutes at 1500 RPM in a Beckman CS-6R centrifuge and 90 μl of supernatant is carefully removed from each well with a micropipette. The pelleted cells are resuspended in 100 μl of hTCM, pooled in sterile tubes (Corning Costar corp sterile ClusterTAb #441 1 , Cambridge, Mass.), mixed and transferred into an equal number of wells of an anti IFN- y capture plate. Capture plates are incubated undisturbed at 37⁹C for 16-20 hours. At the end of the IFN-γ secretion phase, the cells are discarded and the plates are washed three times with 0.1% PBST. A final aliquot of PBST is added to the wells for ten minutes, removed, and 100 μl of a 1 :500 dilution of rabbit anti-human IFN-γ polyclonal antibody (Endogen #P700, Cambridge, Mass.) in PBST+1% BSA is added to each well for 3.5 hours at room temperature with gentle rocking. Unbound anti-IFN-γ polyclonal antibody is removed by three washes with PBST, followed by a wash with 250 μl of 1X Tris-buffered saline+0.05% Tween 20 (TBST). Next, a 100 μl aliquot of 1 :5000 alkaline phosphatase-conjugated mouse anti- rabbit polyclonal antibody (Jackson Immunological #21 1 -055-109, West Grove, Pa.) diluted in TBST is added to each well and incubated at room temperature for 1.5-2 hours with gentle rocking. Excess enzyme-conjugated antibody is removed by three washes with PBST and two washes with alkaline phosphatase buffer (APB=O.1 M NaCI, 0.05 M MgCl.sub.2, 0.1 M Tris HCI, pH 9.5) followed by addition of the substrate mix of p-Toluidine salt and nitroblue tetrazolium chloride (BCIP/NBT, GIBCO BRL

#18280-016, Gaithersburg, Md.). To stop the calorimetric reaction, plates were washed three times in dH₂O, inverted to minimize deposition of dust in the wells, and dried overnight at 28⁹C in a dust free drying oven.

Images of the spots corresponding to the lymphokine secreted by individual antigen- stimulated T cells are captured with a CCD video camera and the image is analyzed by NIH image software. Captured images are enhanced using the Look Up Table which contrasts the images. Thresholding is then applied to every image and a wand tool is used to highlight the border to effectively subtract the edge of the well so that background counts won't be high and artificial. Density slicing over a narrow range is then used to highlight the spots produced from secreting cells. Pixel limits are set to subtract out small debris and large particles, and the number of spots falling within the prescribed pixel range are counted by the software program. Totals from each well are then manually recorded for future analysis. Alternatively, spots can be counted by other commercially available or customized software applications, or may be quantitated manually by a technician using standard light microscopy. Spots can also be counted manually under a light microscope.

We conclude that the protocol detailed above can be used for the enumeration of single IFN-γ secreting T cells.

Example 38

This is an example of measurement of antigen reactive T-CeIIs by IFN-γ capture in blood samples by ELISPOT. This is an example of indirect detection of TCR, where individual cells are immobilized and measured by a chromogen assay. The antigenic peptide origin is a library of antigens.

The example provides a sensitive assay for the detection of T-cells reactive to the antigen of a library generated as described in example 21 , by detecting a soluble factor whose secretion is induced by stimulation of the T-cell by the antigen.

This example is similar to the experiment above. PMBC are isolated, prepared and stored as described in the example above.

The purified PBMCs are plated at 2x10⁵ cells/well at a volume of 0.1 ml in 96 well Costar cell culture plates. An equal volume of antigens from the library, at 10 μg/ml is added to triplicate or sextuplet sets of wells and the plate is incubated in a 37⁹C, 5% CO₂ incubator. On day five, 10 μl/well of 100 U/ml stock recombinant IL-2 is added to each well. On day 8, frozen PBMCs are thawed, washed in DPBS+0.5% BSA to remove DMSO, resuspended to a concentration of 4x10⁶ cells/ml in hTCM, and y- irradiated (3,000 RADS). 50 μl/well are dispensed along with 50 μl of the appropriate antigen at a stock concentration of 40 μl/ml to give a final antigen concentration of 10 μg/ml.

A capture plate with IFN-γ antibody is prepared, washed and blocked as described in the example 37 above.

On day 9 of the assay, twenty four hours after the second antigen stimulation, the stimulation plate is spun for 5 minutes at 1500 RPM and 90 μl of supernatant is carefully removed from each well with a micropipette. The pelleted cells are resuspended in 100 μl of hTCM, pooled in sterile tubes, mixed and transferred into an equal number of wells of an anti IFN- y capture plate. Capture plates are incubated undisturbed at 37^ for 16-20 hours. At the end of the IFN-γ secretion phase, the cells are discarded and the plates are washed three times with 0.1% PBST. A final aliquot of PBST is added to the wells for ten minutes, removed, and 100 μl of a 1 :500 dilution of rabbit anti-human IFN-γ polyclonal antibody in PBST+1% BSA is added to each well for 3.5 hours at room temperature with gentle rocking. Unbound anti-IFN-γ polyclonal antibody is removed by three washes with PBST, followed by a wash with 250 μl of 1X Tris-buffered saline+0.05% Tween 20 (TBST). Next, a 100 μl aliquot of 1 :5000 alkaline phosphatase-conjugated mouse anti-rabbit polyclonal antibody diluted in TBST is added to each well and incubated at room temperature for 1.5-2 hours with gentle rocking. Excess enzyme-conjugated antibody is removed by three washes with PBST and two washes with alkaline phosphatase followed by addition of the substrate mix of p-Toluidine salt and nitroblue tetrazolium chloride. To stop the calorimetric reaction, plates were washed three times in dH₂O, inverted to minimize deposition of dust in the wells, and dried overnight at 28⁹C in a dust free drying oven.

Images of the spots corresponding to the lymphokine secreted by individual antigen- stimulated T cells are captured with a CCD video camera and the image is analyzed as described in the example above

We conclude that the experiment detailed above can be used for the enumeration of single IFN-γ secreting T cells in blood..

Example 39

This is an example of how antigen specific T-cells can be detected using a direct detection method detecting T cell immobilized in solid tissue. In this example MHC dextramers are used to detect antigen specific T cells on frozen tissue sections using enzymatic chromogenic precipitation detection.

Equilibrate the cryosection tissue (e.g. section of spleen from transgenic mice) to -

20⁹C in the cryostate. Cut 5 μm sections and then dry sections on slides at room temperature. Store slides frozen until use at -20⁹C. Equilibrate frozen sections to room temperature. Fix with acetone for 5 min.

Immediately after fixation transfer slides to TBS buffer (50 mM Tris-HCL pH 7,6, 150 mM NaCI) for 10 min.

Incubate slides with FITC-conjugated MHC-dextramers at appropriate dilution (1 :40-

1 :80) and incubate for 30 min at room temperature. Other dilution ranges, as well as incubation time and temperature, may be desirable.

Decant solution and gently tap slides against filter paper, submerge in TBS buffer.

Decant and wash for 10 min in TBS buffer.

Incubate with rabbit polyclonal anti-FITC antibody (Dako P5100) at 1 :100 dilution in

TBS at room temperature for 30 min. Repeat step 5 and 6. Incubate with Envision anti-Rabbit HRP (Dako K4003) at room temperature for 30 min. Other visualization systems may be used. Repeat step 5 and 6.

Develop with DAB+ (Dako K3468) in fume hood for 10 min. Other substrates may be used. Rinse slides in tap-water for 5 min. Counterstain with hematoxylin (Dako S3309) for 2 min. Repeat step 12, mount slides. The slides stained with MHC-Dextramers can now be evaluated by microscopy.

Example 40 This is an example of how antigen specific T-cells can be detected using a direct detection method detecting T cell immobilized in solid tissue. In this example MHC dextramers are used to detect antigen specific T cells on paraffin embedded tissue sections using enzymatic chromogenic precipitation detection.

Formaldehyde fixed paraffin-embedded tissue are cut in section and mounted on the glass slice, for subsequent IHC staining with MHC-dextramers. Tissue fixed and prepared according to other protocols may be used as well. E.g. fresh tissue, lightly fixed tissue section (e.g. tissue fixed in 2% formaldehyde) or formalin-fixed, paraffin- embedded tissue section. Optimal staining may require target retrieval treatment with enzymes as well as heating in a suitable buffer before incubation with antibodies and MHC-dextramer. The sample is stained for DNA using DAPI stain, followed by incubated with an antigen specific MHCdex/FITC reagent, followed by addition of anti-FITC antibody labeled with HRP. Then the substrate for HRP, "DAP" is added and the reaction allows to progress.

The sample is analyzed by light microscopy for the present of a colored precipitate on the cells (DAPI stained nucleus) positive for the specific MHC/dex reagent. A digital image of the stained sample is obtained, and this can be analyzed manually in the same way as by microscopy. However, a digital image may be used for automatic determination of where and how many cells that are positive, related to the total amount of cells, determined by the DAPI staining, or other criteria or stainings. Example 41

This example describes how the quality of a MHC multimer can be tested. The MHC multimer in this example is a MHC-dextramer, and the test involves specific binding of the MHC-dextramer to a cell line that express specific TCRs and display these on the cell surface.

A transfected Jurkat T celle line (JT3A) from Altor Biosciences specific for the MHC complex HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) was evaluated as positive control for the MHC-dextramer HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)). The cells were cultured and treated to express TCR just before evaluation. Under the conditions used, 20-50% of the cells were expected to express and display TCR. After stimulation the cells were incubated with fluorescently labeled MHC-dextramers and the extent of cell staining analyzed by flow cytometry, as follows:

1 . JT3A cells growing in log phase were incubated at room temperature for 2-3 hours to express TCRs (The TCRs are not stable expressed at 37°C).

2. After 3 hours cells were centrifuged for 5 min at 400 g, and the supernatant was discarded.

3. Cells were washed in PBS pH 7.4 + 5% FCS, and centrifuged for 5 min at 400 g. The supernatant was discarded, and cells resuspended in proper volume PBS pH 7.4 + 5% FCS for counting in a Bϋrker chamber.

4. 1 x10⁶ cells per sample in 10Oμl PBS pH 7.4 + 5% FCS were added to each sample tube.

5. 10μl MHC-dextramers were added. Incubation for 30 min at 4^ in the dark.

6. 5μl anti-CD3 was added to each sample. Further incubation for 30 min at 4^ in the dark.

7. Samples were washed in 2 ml PBS, centrifuged for 5 min at 300 g. Supernatant discarded and sample resuspended in 0.4 ml PBS pH 7.4.

8. Samples were kept at 2-8⁹C in the dark until analysis on flow cytometer.

9. Samples were analyzed by flow cytometry on a CyAn instrument.

Data were analyzed by the Summit software. Stimulated JT3A cells were stained with the specific MHC-dextramer HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) and anti- CD3. Another sample of cells were stained with the irrelevant MHC-dextramer HLA- A^*0201 (GILGFVFTL) and anti-CD3. The cells stained with HLA-A^*0201 (GILGFVFTL) had weak signals (low fluorescent intensity), and therefore regarded as the negative population. A boundary was introduced in the dot plot, to mark the negative population. Cells with fluorescence higher than the negative boundary were hereafter regarded positive. 19% and 0.25% of the cells were regarded positive when stained with the relevant and irrelevant MHC-dextramer, respectively. See table below.

The results thus correlate well with the expected 20-50% HLA-A^*0201 (NLVPMVATV (SEQ ID NO 9672)) positive JT3A cells after stimulation. We conclude that the transfected Jurkat cell line (JT3A) can be used as positive control for the MHC- dextramer.

Example 42 This example describes how the quality of a MHC multimer can be tested. The MHC multimer in this example is a MHC-dextramer, and the test involves specific binding of the MHC-dextramer to cell preparations expressing TCRs.

Three different peptide specific T-cell preparations of Human cytotoxic T lymphocyte lines specific for a viral peptides were incubated with fluorescently labeled MHC- dextramers and the extent of cell staining analyzed by flow cytometry. The following T- cell preparations were examined: (NLV) specific for MHC-dextramer HLA- A^*0201 (NLVPMVATV (SEQ ID NO 9672)), (IPSI) specific for MHC-dextramer B^*3501 (IPSINVHHY) and (GLC) specific for MHC-dextramer A^*0201 (GLCLVALM).

1. Cells were added 1 ml RPMI and then transfer to a tube with 9ml RPMI. Cells were centrifuged for 5 min at 300 g, and the supernatant was discarded.

2. Cells were washed in 10ml PBS pH 7.4 + 5% FCS, and centrifuged for 5 min at 300 g, and the supernatant was discarded. 3. 1 x10⁶ cells per sample in 10Oμl PBS pH 7.4 + 5% FCS were added to sample tubes.

4. 10μl MHC Dextramers were added, and incubated at room temperature in the dark for 10 min.

5. 5μl anti-CD3 and anti-CD8 were added to each sample. Further incubation for 20 min at 4°C in the dark.

6. Samples were washed in 2ml PBS pH 7.4 + 5% FCS and centrifuged for 5 min at 300 g, and the supernatant was discarded.

7. Pellets were resuspended in 0.4 ml PBS pH 7.4.

8. Samples were kept in the dark at 2-8⁹C until analysis on a flow cytometer.

9. Samples were analyzed by flow cytometry on a CyAn instrument.

Data were analyzed by the Summit software. The cell preparations were stained with anti-CD3, anti-CD8, the respective specific MHC-dextramer, or an irrelevant MHC- dextramer. Anti-CD3 positive cells were positively gated and anti-CD8 vs. MHC- dextramer were depicted in a dot plot. The main population of anti-CD8 positive cells stained with the irrelevant MHC-dextramer was regarded as negative, and a boundary was introduced in the dot plot to mark the negative population. Anti-CD8 positive cells with fluorescence higher than the negative boundary were regarded positive. In the NLV and IPSI cell preparations, approximately 95% of the CD8⁺ cells were positive for the relevant MHC dextramer. 45% of the CD8⁺ GLC cells were positive for relevant MHC Dextramers, see table below. Cell preparations were not stained by the irrelevant MHC-dextramer.

We conclude that the different peptide specific T-cell preparations can be used as positive controls for the relevant MHC-dextramer.

Example 43

This example describes the prediction of MHC class 1 and 2 CMV specific antigenic peptide sequences for use in construction of MHC multimers designed to be used for analytical, diagnostic, prognostic, therapeutic and vaccine purposes, through the interaction of the MHC multimers with CMV specific T-cells. Prediction of the 8-, 9-, 10-, 1 1 -, 13-, 14-, 15- and 16-mer peptide sequences are carried out using the protein sequence for the human CMV derived antigen pp65 (see table 6) and the peptide generation software program described in figure 2. The outcome is shown in Table 9 under the pp65 antigen.

Example 44

This is an example of how MHC multimers may be used for the detection of antigen specific T-cells simultaneously with activation of T cells.

This example is a combination of i) direct detection of TCR, using MHC complexes coupled to any multimerisation as described elsewhere herein to stain antigen specific T cells, and ii) indirect detection of TCR, by detection of induced intracellular cytokine production by addition of fluorophor-labelled anti-cytokine antibodies by flow cytometry.

Multicolor immunofluorescent staining with antibodies against intracellular cytokines and cell surface markers provides a high resolution method to identify the nature and frequency of cells which express a particular cytokine(s). In addition to enabling highly specific and sensitive measurements of several parameters for individual cells simultaneously, this method has the capacity for rapid analysis of large numbers of cells which are required for making statistically significant measurements.

Production of cytokines plays an important role in the immune response. Examples include the induction of many antiviral proteins by IFN-γ, the induction of T cell proliferation by IL-2 and the inhibition of viral gene expression and replication by TNF- α. Cytokines are not preformed factors; instead they are rapidly produced upon relevant stimulation. Intracellular cytokine staining relies upon the stimulation of T cells in the presence of an inhibitor of protein transport thus retaining the cytokines inside the cell. Cellular activation to trigger cytokine production generally results in down-regulation of the T cell receptor. For this reason, MHC multimer staining is carried out prior to activation to ensure a good level of staining. The MHC multimers may be internalized with the T cell receptor during this period, but can still be detected in permeabilized cells. To analyze the effector function of antigen-specific T cells, the cells are first stained with MHC multimers, and then stimulated with antigen. This is followed by staining with antibodies specific for extracellular epitopes (such as CD8), then by membrane permeabilization and intracellular cytokine staining. The following protocol is an example of MHC multimer co-staining with anti-IFN-γ, TNF-α, MIP-I b, or IL-2.

Protocol applicable for intracellular staining of IFN-gamma, TNFa, MIP-I b, or IL-2

1 . Prepare peripheral blood cells in phosphate buffered saline (PBS) at a cell concentration of 2x10⁷ cells/ml.

2. Transfer the cell suspension to individual tubes in 50 μl aliquots.

3. Add relevant titrated fluorescently-labeled MHC multimers to the desired tubes, and incubate for 10 min at 22^QC (nonstimulated single-color controls should not be stained at this stage). Add 10 μl PBS to remaining tubes.

4. Add 500 μl PBS to each tube. Centrifuge at 450 x g for 5 minutes at 10^<€.

5. Aspirate supernatant. Agitate to disrupt cell pellets and resuspend in 200 μl complete RPMI. 6. Dilute peptide/antigen stock 1 :50 in complete RPMI. Add 2 μl of this (10 μg/ml

(investigate the effect on cytokine response of titrating your peptide)) to each desired tube. If using Leukocyte Activation cocktail (LAC) as a control, rapidly thaw this at 37^ in a water bath and add 0.33 μl of this to each desired tube. 7. Place the tubes at 37^ in a humidified CO₂ incubator for 15 minutes to 1 hour. 8. Add Brefeldin A (10 μg/ml final) to the desired tubes (n.b. LAC contains Brefeldin A) and return to the incubator. Incubate for 15 hours (the optimal incubation time isvariable and must be determined).

9. Remove tubes from the incubator. Centrifuge at 450 x g for 5 minutes at 10⁹C.

10. Aspirate supernatant. Resuspend desired cell pellets in 50 μl PBS containing an optimally titrated amount of anti-CD8 antibody. Add 50 μl PBS to remaining tubes. Note: Single-color controls should be stained at this stage. If additional phenotyping of samples is desired, antibodies to other cell surface receptors may also be added at this time.

1 1. Incubate for 20 minutes on ice. 12. Add 500 μl PBS to each tube. Centrifuge at 450 x g for 5 minutes at 10°C.

13. Aspirate supernatant. Agitate to disrupt cell pellets.

14. Add 200 μl 4% paraformaldehyde to each sample tube. Vortex tubes. Incubate for 20 minutes on ice. This step will fix the cell morphology of the activated cells.

Note: The procedure can be stopped at this point. Repeat steps 12 and 13. Resuspend the cells in 100 μl/tube PBS. Cover and store the cells at 4^ for up to 3 days. To proceed, repeat steps 12 and 13. Resuspend the cells in 100 μl/tube permeabilization buffer and proceed to step 16.

15. Add 200 μl permeabilization buffer to each tube.

16. Centrifuge at 450 x g for 5 minutes at 10⁹C. Aspirate supernatant. 17. Add 100 μl permeabilization buffer to the sample tubes that are to be stained with anti-cytokine antibody. Add 100 μl PBS to the remaining tubes (i.e. Single-color controls).

18. Incubate for 5 minutes at room temperature.

19. Add an optimally titrated amount of conjugated anti-cytokine antibody to the desired sample tubes and mix.

20. Incubate for 20 minutes at room temperature.

21. Add 200 μl permeabilization buffer to each tube and centrifuge at 450 x g for 5 minutes at 10⁹C. Aspirate supernatant and agitate tubes to disrupt the cell pellets.

22. Resuspend the cells in 200 μl fix solution. Vortex tubes. It is important to vortex well when adding this fixative so that cells do not clump.

23. The samples are now ready for data acquisition and analysis on a flow cytometer but may be stored overnight at 4^ in the dark prior to analysis.

We conclude that the MHC multimer constructs can be used to detect the presence of specific T cells in the blood simultaneously with activation and intracellular staining of cytokines.

Example 45

This is an example of how MHC multimers may be used for the detection of antigen specific T-cells simultaneously with activation of T cells. This example is a combination of i) direct detection of TCR, using MHC complexes coupled as pentamer structures to stain antigen specific T cells, and ii) indirect detection of TCR, by detection of induced intracellular cytokine production by addition of fluorophor-labelled anti-cytokine antibodies by flow cytometry. The antigenic origin is Epstein-Barr Virus (EBV), thus, immune monitoring of EBV infection

PBMCs were incubated with either a negative control (non-specific) Pentamer MHC multimer (A^*0201/EBV (GLCTLVAML (SEQ ID NO 9675))) or a Pentamer MHC multimer specific for the cells of interest (B^*0801/EBV (RAKFKQLL)), then stimulated with LAC (non-specific activation) or B^*0801/EBV peptide (specific peptide activation) for 15 hours in the presence of Brefeldin A. Pentamer MHC multimers were produced as described elsewhere herein. Fixation, permeabilization and staining for IFN-γ were carried out exactly as detailed in the protocol outlined in example 44 above.

Figure 26 illustrates Pentamer (specific or non-specific) versus intracellular IFN-γ staining after activation with specific or non-specific antigen.

We conclude that the MHC multimer constructs can be used to detect the presence of EBV specific T cells in the blood simultaneously with activation and intracellular staining of cytokines.

Modified from www.proimmune.com: Pro5 Recombinant MHC Pentamer staining protocol for human Intracellular Proteins. Version 4.1 02/2007.

Example 46 This is an example of how MHC multimers may be used for the detection of antigen specific T-cells and activation of T cells

This example is a combination of i) direct detection of TCR, using MHC complexes coupled as any multimerisation as described elsewhere herein to stain antigen specific T cells, and ii) indirect detection of TCR, by detection of induced intracellular cytokine production by addition of fluorophor-labelled anti-cytokine antibodies by flow cytometry.

PBMCs are stimulated with either a negative control (non-specific) MHC multimer or a MHC multimer carrying a CMV derived antigenic peptide for an optimal period of time in the presence of Brefeldin A, thereby stimulating CMV specific T cells. Fixation, permeabilization and staining for IFN-γ are carried out as detailed in the protocol outlined in the example 44.

We conclude that the MHC multimer constructs can activate T cells. The cytokine production is detected by intracellular staining in flow cytometric analysis.

Example 47

This is an example of how MHC tetramers may be used to monitor the immune status of a patient following transplantation, thereby guiding the immune suppressive treatment. The detection method used is a direct detection of individual specific T cells using flow cytometry.

T lymphocytes (T cells) play a critical role in host immune defense to microbial infections. Specialized phagocytic cells, known as antigen-presenting cells (APCs), process foreign proteins resulting in peptide fragment expression on their cell surfaces. These peptides are complexed to MHC (major histocompatibility complex) molecules. When naϊve T cells encounter APCs expressing foreign peptide-MHC molecules, the T cells are induced to differentiate and proliferate. The result is a cellular immune response consisting of T cells that are capable of recognizing and destroying infected cells expressing the specific peptide-MHC molecule. Long-term memory cells capable of rapidly responding to a repeat infection are also generated. These antigen-specific T cells can be detected using MHC Tetramers. Within their lifetime, more than half of the world's population will become infected with human cytomegalovirus (CMV), a member of the ubiquitous herpes virus family. Once an individual is infected, viral particles can escape total immunoclearance and remain dormant within the host's cells. CMV antigen-specific CD8+ cytotoxic T cells can control the latent CMV infection in healthy individuals.

The detection of CD8+ antigen-specific T cells requires cognate recognition of the T cell receptor (TCR) by a unique combination of a Class I major histocompatibility complex (MHC) molecule coupled with a specific antigen peptide. CMV-specific TCR on the surface of CD8+ T cells is recognized by MHC Class I Tetramers carrying CMV antigenic peptides in the peptide binding cleft. MHC Class I Tetramers are a complex of four peptide-MHC Class I molecules stably bound with streptavidin to which a fluorochrome (most often PE) is attached. MHC Class I Tetramers are used in combination with fluorescently conjugated CD3 and CD8 antibodies to determine the frequency of CD3+CD8+ T cells.

The following flow cytometric protocol describes a two-panel technique for the determination of the absolute count (cells/μL) of CMV antigen-specific CD8+ T cells in whole blood. A similar approach has been used by the European Working Group on

Clinical Cell Analysis. Panel 1 consists of a single assay tube containing whole blood, anti-CD3, anti-CD4, anti-CD8 monoclonal antibody reagents, and Flow-Count Fluorospheres and is prepared using a lyse-no-wash method. Results from panel 1 provide a direct determination of the

CD3+CD4+ and CD3+CD8+ T cell subset absolute counts. Panel 2 consists of a variable number of tubes dependent on the MHC or HLA (Human Leukocyte Antigen) phenotype of the individual being tested.

Each tube in panel 2 contains whole blood, anti-CD3, anti-CD8, and specific CMV MHC Class I Tetramer and is prepared using a lyse-with-wash method. Results from panel 2 are used to determine the relative percentage of antigen-specific CD3+CD8+ T cells present within a sample.

Identification of the CD3+CD8+ population is common to both panels. Therefore, by applying the percentage of antigen-specific CD3+CD8+ T cells determined in panel 2 to the absolute CD3+CD8+ cell count determined in panel 1 , the absolute number of antigen-specific CD3+CD8+ T cells is determined per μL of whole blood. Both panels may be prepared concomitantly.

Control Cells are prepared as described above for use in panel 1 only.

3

Detection and Enumeration of CMV Antigen-Specific CD8-Positive T Lymphocytes in

Whole Blood by Flow Cytometry.

Reagent Preparation 1 . Lyse/Fixative solution: Calculate the total volume of Lyse/Fixative solution required

(panel 1 — 1 mL/tube. Add 25 μL of MHC Tetramer Fixative Reagent to 1 mL of iTAg

MHC Tetramer Lyse Reagent.

2. 0.1% formaldehyde in PBS: Calculate the total volume of 0.1 % formaldehyde/PBS fixative solution required (panel 2 only — 0.5 ml_/tube). Add 12.5 μL iTAg MHC Tetramer Fixative Reagent to 1 mL of PBS. 3. Bring all monoclonal antibody reagents, IMMUNO-TROL™ Control Cells and iTAg MHC Tetramers to room temperature (RT) before pipetting. Vortex before use.

4. On the same day of data acquisition by flow cytometry, remove Flow-Count™ Fluorospheres from 4°C storage. Bring to RT prior to use. Vortex for 10-12 seconds and avoid excessive mixing to minimize air bubble formation.

Panel 1. Determination of Absolute T Cell Counts — Lyse-no-Wash

1. Appropriately label tubes for each patient being tested.

2. Pipette 10 μL of anti-CD3, 10 μL of anti-CD4, and 10 μL of anti-CD8 into the bottom of each tube.

3. Pipette 100 μL of whole blood into the bottom of each tube.

4. Vortex gently to ensure complete mixing of whole blood sample with antibody reagents.

5. Incubate tubes at room temperature (18-25⁹C) for 20-30 minutes, protected from light.

6. Add 1 ml_ of Lyse/Fixative solution to each tube and vortex immediately for one second after each addition.

7. Incubate at room temperature for at least 10 minutes, protected from light.

8. Store prepared samples at 4^, protected from light, until the addition of Flow-Count Fluorospheres within 24 hours.

9. Pipette 100 μL of adequately mixed room-temperature Flow-Count Fluorospheres into each tube immediately prior to analysis by flow cytometry.

10. Vortex each tube for 5 seconds to ensure proper mixing and resuspension of cells and fluorospheres.

1 1 . Samples must be analyzed within one hour. Repeat vortexing immediately prior to flow-cytometric acquisition.

Panel 2. Determination of Relative Percent of CMV Antigen-Specific CD8+ T Cells — Lyse-with-Wash

1. Appropriately label tubes for each patient being tested.

2. Add 10 μL of specific iTAg MHC Class I Tetramer or Negative Tetramer and 10 μL each of anti-CD3 and anti-CD8 monoclonal antibody reagents into each tube.

3. Add 200 μL of whole blood into each tube. Specimens with low leukocyte counts (<3.0 x 103/μL) or low lymphocyte counts (<0.5 x 103/μL) may require whole blood volumes up to 400 μl_. Under these circumstances, up to 4 ml_ of Lyse/Fixative solution is required— all other reagent volumes remain as described.

4. Vortex gently.

5. Incubate at room temperature (18-25⁹C) for 20-30 minutes, protected from light. 6. Add 2 ml_ of Lyse/Fixative solution to each tube and vortex immediately for one second after each addition.

7. Incubate at room temperature for at least 10 minutes, protected from light.

8. Centrifuge tubes at 150 x g for 5 minutes.

9. Aspirate or decant the supernatant. 10. Add 3 ml_ of PBS.

1 1. Centrifuge tubes at 150 x g for 5 minutes.

12. Aspirate or decant the supernatant.

13. Resuspend the cell pellet in 500 μl_ of PBS with 0.1% formaldehyde.

14. Vortex each tube for 5 seconds. 15. Store prepared samples at 4°C protected from light for a minimum of 1 hour (maximum 24 hours) until analysis by flow cytometry.

Example 48

This is an example of how MHC dextramers may be used to monitor the immune status of a patient following transplantation, thereby guiding the immune suppressive treatment. The detection method used is a direct detection of individual specific T cells using flow cytometry.

T lymphocytes (T cells) play a critical role in host immune defense to microbial infections. Specialized phagocytic cells, known as antigen-presenting cells (APCs), process foreign proteins resulting in peptide fragment expression on their cell surfaces.

These peptides are complexed to MHC (major histocompatibility complex) molecules.

When naϊve T cells encounter APCs expressing foreign peptide-MHC molecules, the T cells are induced to differentiate and proliferate. The result is a cellular immune response consisting of T cells that are capable of recognizing and destroying infected cells expressing the specific peptide-MHC molecule. Long-term memory cells capable of rapidly responding to a repeat infection are also generated.

These antigen-specific T cells can be detected using MHC Dextramers.

Within their lifetime, more than half of the world's population will become infected with human cytomegalovirus (CMV), a member of the ubiquitous herpes virus family. Once an individual is infected, viral particles can escape total immunoclearance and remain dormant within the host's cells. CMV antigen-specific CD8+ cytotoxic T cells can control the latent CMV infection in healthy individuals.

The detection of CD8+ antigen-specific T cells requires cognate recognition of the T cell receptor (TCR) by a unique combination of a Class I major histocompatibility complex (MHC) molecule coupled with a specific antigenic peptide. CMV-specific TCR on the surface of CD8+ T cells is recognized by fluorescent labeled MHC Class I Dextramers carrying CMV antigenic peptides in the peptide binding celft. MHC Class I Dextramers are used in combination with fluorescently conjugated CD3 and CD8 antibodies to determine the frequency of CD3+CD8+ T cells.

The following flow cytometric protocol describes a two-panel technique for the determination of the absolute count (cells/μL) of CMV antigen-specific CD8+ T cells in whole blood. A similar approach has been used by the European Working Group on Clinical Cell Analysis. Panel 1 consists of a single assay tube containing whole blood, anti-CD3, anti-CD4, anti-CD8 monoclonal antibody reagents, and Flow-Count Fluorospheres and is prepared using a lyse-no-wash method. Results from panel 1 provide a direct determination of the CD3+CD4+ and CD3+CD8+ T cell subset absolute counts. Panel 2 consists of a variable number of tubes dependent on the MHC or HLA (Human Leukocyte Antigen) phenotype of the individual being tested. Each tube in panel 2 contains whole blood, anti-CD3, anti-CD8, and specific CMV MHC Class I Dextramer and is prepared using a lyse-with-wash method. Results from panel 2 are used to determine the relative percentage of antigen-specific CD3+CD8+ T cells present within a sample.

Identification of the CD3+CD8+ population is common to both panels. Therefore, by applying the percentage of antigen-specific CD3+CD8+ T cells determined in panel 2 to the absolute CD3+CD8+ cell count determined in panel 1 , the absolute number of antigen-specific CD3+CD8+ T cells is determined per μL of whole blood. Both panels may be prepared concomitantly. IMMUNO-TROL Control Cells are prepared as described above for use in panel 1 only.

Detection and Enumeration of CMV Antigen-Specific CD8-Positive T Lymphocytes in Whole Blood by Flow Cytometry. Reagent Preparation

1 . Lyse/Fixative solution: Calculate the total volume of Lyse/Fixative solution required (panel 1 — 1 mL/tube, panel 2 — 2 mL/tube). Add 25 μl_ of MHC Dextramer Fixative Reagent to 1 ml_ of MHC Dextramer Lyse Reagent. 2. 0.1% formaldehyde in PBS: Calculate the total volume of 0.1 % formaldehyde/PBS fixative solution required (panel 2 only — 0.5 ml_/tube). Add 12.5 μl_ MHC Dextramer Fixative Reagent to 1 ml_ of PBS.

3. Bring all monoclonal antibody reagents, IMMUNO-TROL™ Control Cells and MHC Dextramers to room temperature (RT) before pipetting. Vortex before use. 4. On the same day of data acquisition by flow cytometry, remove Flow-Count™

Fluorospheres from 4°C storage. Bring to RT prior to use. Vortex for 10-12 seconds and avoid excessive mixing to minimize air bubble formation.

Panel 1. Determination of Absolute T Cell Counts — Lyse-no-Wash 1 . Appropriately label tubes for each patient being tested.

3. Pipette 100 μL of whole blood into the bottom of each tube.

6. Add 1 ml_ of Lyse/Fixative solution to each tube and vortex immediately for one second after each addition. 7. Incubate at room temperature for at least 10 minutes, protected from light.

8. Store prepared samples at 4⁹C, protected from light, until the addition of Flow-Count Fluorospheres within 24 hours.

1 1. Samples must be analyzed within one hour. Repeat vortexing immediately prior to flow-cytometric acquisition. o

1 . Appropriately label tubes for each patient being tested.

2. Add 10 μL of specific MHC Class I Dextramer or Negative Dextramer and 10 μL each of anti-CD3 and anti-CD8 monoclonal antibody reagents into each tube.

3. Add 200 μL of whole blood into each tube. Specimens with low leukocyte counts (<3.0 x 103/μL) or low lymphocyte counts (<0.5 x 103/μL) may require whole blood volumes up to 400 μL. Under these circumstances, up to 4 mL of Lyse/Fixative solution is required — all other reagent volumes remain as described. 4. Vortex gently.

5. Incubate at room temperature (18-25⁹C) for 20-30 minutes, protected from light.

6. Add 2 mL of Lyse/Fixative solution to each tube and vortex immediately for one second after each addition.

7. Incubate at room temperature for at least 10 minutes, protected from light. 8. Centrifuge tubes at 150 x g for 5 minutes.

9. Aspirate or decant the supernatant.

10. Add 3 mL of PBS.

1 1. Centrifuge tubes at 150 x g for 5 minutes.

12. Aspirate or decant the supernatant. 13. Resuspend the cell pellet in 500 μL of PBS with 0.1% formaldehyde.

14. Vortex each tube for 5 seconds.

15. Store prepared samples at 4°C protected from light for a minimum of 1 hour (maximum 24 hours) until analysis by flow cytometry.

Example 49

Any MHC multimer may be used to monitor the immune status of a patient following transplantation, thereby guiding the immune suppressive treatment. The procedure for the use of MHC tetramers to monitor immune status of a patient following transplantation described elsewhere herein may be followed replacing MHC tetramers with MHC dextramers or any other MHC multimer usefull in flow cytometry assays.

Example 50

This is an example of generation of MHC multimers where the multimerization domain is Dextran with Streptavidin (SA) covalently attached. Chemically biotinylated MHC- peptide molecules were coupled to the Dextran-SA multimerization domain through non-covalent interactions between biotin and SA. The dextran multimerization domain also had fluorochrome molecules covalently attached.

The MHC-peptide molecules were B^*0801 molecules binding the CMV derived antigenic peptide ELRRKMMYM or the nonsense peptide AAKGRGAAL in the peptide binding groove. The flurochrome used was PE.

Chemically biotinylated MHC-peptide molecules were generated as follows: Recombinant B^*0801 Heavy chain molecules with a C-terminal Cysteine residue were made by generation of a DNA expression vector encoding the B^*0801 molecule (without the intracellular and transmembrane domains) fused to the sequence GSLHHILDAQKMVWNHRCG. This expression vector was transformed into BL21 DE3 bacteria. Likewise a DNA expression vector encoding human beta2microglobulin (β2m) were generated and transformed into BL21 DE3 bacteria. β2m and the B^*0801 -Cys fusion protein were expressed and purified from inclusion bodies using standard procedures as described in Garboczi et al. (1996), Nature 384, 134-141. MHC-peptide complexes was generated by in vitro refolding of B^*0801 -cys fusion protein, β2m and either of the peptides ELRRKMMYM or AAKGRGAAL in a buffer containing reduced and oxidized gluthatione using the following steps: 17. 100 ml of refolding buffer (100 mM Tris, 400 mM L-arginin-HCL, 2 mM NaEDTA, 0.5 mM oxidized Gluthathione, 5 mM reduced Glutathione, pH 8.0) was supplied with protease inhibitors PMSF (phenylmethylsulphonyl fluoride), Pepstatin A and

Leupeptin (to a final concentration of 1 mM, 1 mg/l and 1 mg/l, respectively). The refolding buffer was placed at 10 ⁰C.

18. 6 mg of peptide was dissolved in DMSO (200-300 μl), and added drop-wise to the refolding buffer at vigorous stirring. 19. 6,6 mg of β2m was added drop-wise to the refolding buffer at vigorous stirring.

20. 9,4 mg of Heavy Chain HLA-B^*0801 (supplied with DTT to a concentration of 0.1 mM) was added drop-wise to the refolding buffer at vigorous stirring.

21 . The folding reaction was placed at 10⁰C at slow stirring for 18 hours.

22. The folding reaction was filtrated through a 0.45 μm filter to remove aggregates and then concentrated to approximately 5 ml using a 20 ml Vivaspin concentrator with a

5000 mw cut-off filter.

23. Correctly folded MHC-complex was separated and purified from excess β2m, excesss heavy chain and aggregates thereoff, by size exclusion chromatography on a column that separates proteins in the 10-100 kDa range. Correctly folded monomer MHC-complex was eluted with a MHC-buffer (20 mM Tris-HCI, 50 mM NaCI, pH 8.0).

24. Fractions containing the folded monomer MHC-peptide complex were pooled, the buffer exchanged to PBS, pH = 7.0 and the sample concentrated to approximately 1 ml in a suitable concentrator with a 5000 mw cut-off filter. The protein- concentration was estimated from its absorption at 280 nm.

Correct foldning of purified B^*0801 -cys-peptide molecules were verified as described in example 34. Then the molecules were chemically biotinylated by treating purified B^*0801 -cys-peptide with Maleimide-biotin (EZ-link Maleimide-PEO2-Biotin). The reaction was carried out in PBS, PH 7.0 for 2¹/2 hour at 4°C, with a Maleimide-biotin: B^*0801 -cys-peptide molar ratio equal to 1.5 (1 ,6 ug Maleimide-biotin per 100 ug B^*0801 -cys-peptide complex). Excess Maleimide-biotin was removed and the buffer exchanged to 20 mM Tris-HCI, 50 mM NaCI, pH 8.0 by gel filtration in a G-25 column. Efficiency of biotinylation was determined using a SDS PAGE shift assay with SA. 1 ug Maleimide-biotin treated B^*0801 -cys-peptide complex was incubated with 1.8 ug SA in 12 ul PBS, pH 7.0 for 1 hour at room temperature. Then the sample was analyzed by SDS PAGE together with samples of various concentrations of Maleimide-biotin treated B^*0801 -cys-peptide complex not incubated with SA and a sample with SA alone. The degree of biotinylation was estimated to approximately 70% (see figure 27).

Biotinylated B^*0801 -cys-peptide was then coupled to RPE-SA-conjugated 27OkDA dextran in a molar ratio of MHC-peptide complexes: SA equal to 3: 1 . The RPE-SA- conjugated 27OkDA dextran was generated as described in example 6 and 7 herein and contained 5.4 mol SA and 3,3 mol RPE per mol dextran. The coupling of biotinylated B^*0801 -cys-peptide to RPE-SA-conjugated 27OkDA dextran was done by mixing 6,79 ul biotinylated B^*0801 -cys-peptide (0.377 mg/ml) with 20 ul RPE-SA- conjugated 27OkDA dextran (16x10⁸ M) followed by incubation for 30 minutes at room temperature in the dark. Then buffer ( 0.05M Tris-HCI, 15mM NaN₃, 1 % BSA, pH 7.2) was added to a total volume of 100 ul. The binding of the above described MHC(peptide)/RPE dextran complexes: B^*0801 - cys- ELRRKMMYM/RPE and B^*0801 -cys- AAKGRGAAL/RPE was used to determine the presence of CMV pp65 specific T cells in the blood from a HLA-B^*0801 positive CMV infected individual by flow cytometry following a standard flow cytometry protocol. Blood from a CMV infected individual was withdrawn and HPBMC isolated by ficoll- Hypaque purification using a standard procedure. Approximately 1x10⁶ purified HPBMC at a concentration of 2x10⁷ cells/ml were incubated with 10 ul of either of the above described dextramer constructs: B^*0801 -cys- ELRRKMMYM/RPE or B^*0801 - cys- AAKGRGAAL/RPE for 10 minutes at 4°C in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human CD3/APC (clone UCHT1 from Dako), mouse-anti-human CD4/FITC (clone MT310 from Dako) and mouse-anti-human CD8/PB (clone DK25 from Dako) were added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples were then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 300xg and the supernatant removed. The washing step was repeated twice. The washed cells were resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flowcytometer.

Staining with B^*0801 -cys-ELRRKMMYM/RPE on a sample from a B^*0801 positive and CMV-infected individual resulted in identification of CD8 positive T cells specific for this Dextramer and thereby the presence of CMV specific T cells, while no MHC Dextramer positive staining was observed with the negative control Dextramer carrying the nonsense-peptide AAKGRGAAL (B^*0801 -cys- AAKGRGAAL/RPE). Results are shown in figure 28.

Staining with B^*0801 -cys-ELRRKMMYM/RPE Dextramer on samples from HLA- mismatched donors (I.e. donors not carrying the B^*0801 allele resulted in no MHC Dextramer positive staining of CD8 positive cells (data not shown). This example demonstrates that B^*0801 -cys Dextramers can be used for identification of CMV specific CD8 positive T cells if the Dextramer carries a CMV derived antigenic peptide in the peptide binding cleft.

Example 51 This is an example of generation of MHC multimers where the multimerization domain is Dextran with Streptavidin (SA) covalently attached. Chemically biotinylated MHC- peptide molecules were coupled to the Dextran-SA multimerization domain through non-covalent interactions between biotin and SA. The dextran multimerization domain also had fluorochrome molecules covalently attached. The MHC-peptide molecules were:

A^*0101 molecules binding the CMV derived antigenic peptide VTEHDTLLY, B^*0702 molecules binding the CMV derived antigenic peptide RPHERNGFTVL, B^*0702 molecules binding the CMV derived antigenic peptide TPRVTGGGAM or B^*0801 molecules binding the nonsense peptide AAKGRGAAL in the peptide binding groove.

The flurochrome used was PE.

Chemically biotinylated MHC-peptide molecules were generated as follows: Recombinant Heavy chain molecules with a C-terminal Cysteine residue were made by generation of a DNA expression vector encoding the Heavy chain molecule (without the intracellular and transmembrane domains) fused to the sequence GSLHHILDAQKMVWNHRCG. This expression vector was transformed into BL21 DE3 bacteria. Likewise a DNA expression vector encoding human beta2microglobulin (β2m) were generated and transformed into BL21 DE3 bacteria. β2m and the Heavy chin- Cys fusion protein were expressed and purified from inclusion bodies using standard procedures as described in Garboczi et al. (1996), Nature 384, 134-141. MHC-peptide complexes was generated by in vitro refolding of Heavy chain-cys fusion protein, β2m and either of the peptides in a buffer containing reduced and oxidized gluthatione using the following steps:

1. 100 ml of refolding buffer (10O mM Tris, 400 mM L-arginin-HCL, 2 mM NaEDTA, 0.5 mM oxidized Gluthathione, 5 mM reduced Glutathione, pH 8.0) was supplied with protease inhibitors PMSF (phenylmethylsulphonyl fluoride),

Pepstatin A and Leupeptin (to a final concentration of 1 mM, 1 mg/l and 1 mg/l, respectively). The refolding buffer was placed at 10 ⁰C.

2. 6 mg of peptide was dissolved in DMSO (200-300 μl), and added drop-wise to the refolding buffer at vigorous stirring.

3. 6,6 mg of β2m was added drop-wise to the refolding buffer at vigorous stirring.

4. 9,4 mg of Heavy Chain (supplied with DTT to a concentration of 0.1 mM) was added drop-wise to the refolding buffer at vigorous stirring.

5. The folding reaction was placed at 10⁰C at slow stirring for 18 hours. 6. The folding reaction was filtrated through a 0.45 μm filter to remove aggregates and then concentrated to approximately 5 ml using a 20 ml Vivaspin concentrator with a 5000 mw cut-off filter.

7. Correctly folded MHC-complex was separated and purified from excess β2m, excesss heavy chain and aggregates thereoff, by size exclusion chromatography on a column that separates proteins in the 10-100 kDa range. Correctly folded monomer MHC-complex was eluted with a MHC-buffer (20 mM Tris-HCI, 50 mM NaCI, pH 8.0). 8. Fractions containing the folded monomer MHC-peptide complex were pooled, the buffer exchanged to PBS, pH = 7.0 and the sample concentrated to approximately 1 ml in a suitable concentrator with a 5000 mw cut-off filter. The protein-concentration was estimated from its absorption at 280 nm.

Correct foldning of purified MHC-cys-peptide molecules were verified as described in example 34. Then the molecules were chemically biotinylated by treating purified MHC- cys-peptide molecules with Maleimide-biotin (EZ-link Maleimide-PEO2-Biotin). The reaction was carried out in PBS, PH 7.0 for 2¹/2 hour at 4°C, with a Maleimide-biotin: MHC-cys-peptide molecule molar ratio equal to 1.5 (1 ,6 ug Maleimide-biotin per 100 ug MHC-cys-peptide molecules). Excess Maleimide-biotin was removed and the buffer exchanged to 20 mM Tris-HCI, 50 mM NaCI, pH 8.0 by gel filtration in a G-25 column. Efficiency of biotinylation was determined using a SDS PAGE shift assay with SA. 1 ug Maleimide-biotin treated MHC-cys-peptide molecules was incubated with 1 .8 ug SA in 12 ul PBS, pH 7.0 for 1 hour at room temperature. Then the sample was analyzed by SDS PAGE together with samples of various concentrations of Maleimide-biotin treated MHC-cys-peptide molecules not incubated with SA and a sample with SA alone. The degree of biotinylation was estimated to approximately 70-90%.

Biotinylated MHC-cys-peptide molecules were then coupled to PE-SA-conjugated 27OkDA dextran in a molar ratio of MHC-peptide complexes: SA equal to 3: 1. The PE- SA-conjugated 27OkDA dextran was generated as described in example 6 and 7 herein and contained 5.4 mol SA and 3,3 mol PE per mol dextran. The coupling of biotinylated MHC-cys-peptide molecules to PE-SA-conjugated 27OkDA dextran were done by mixing biotinylated MHC-cys-peptide molecules with PE-SA-conjugated 27OkDA dextran (16x10⁸ M) followed by incubation for 30 minutes at room temperature in the dark. Then buffer (0.05M Tris-HCI, 15mM NaN₃, 1% BSA, pH 7.2) was added.

A^*0101 (VTEHDTLLY) 70,9 ul (0,41 mg/ml) + 231 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1 155 ul

B^*0702(RPHERNGFTVL) 70,1 ul (0,49 mg/ml) + 273 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1365 ul.

B^*0702(TPRVTGGGAM) 69,5 ul (0,57 mg/ml) + 315 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1575 ul. The binding of the above described MHC-cys-peptide molecules /PE dextran complexes were used to determine the presence of CMV pp50 or pp65 specific T cells in the blood from HLA-matched CMV infected individual by flow cytometry following a Standard flow cytometry protocol. Blood from CMV infected individual was withdrawn and HPBMC isolated by ficoll-Hypaque purification using a Standard procedure. Approximately 1 x10⁶ purified HPBMC at a concentration of 2x10⁷ cells/ml were incubated with 10 ul of either of the above described dextramer constructs for 10 minutes at 4°C in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human CD3/APC (clone UCHT1 from Dako), mouse-anti-human CD4/FITC (clone MT310 from Dako) and mouse-anti-human CD8/PB (clone DK25 from Dako) were added and the incubation continued for another 20 minutes at 4⁰C in the dark. The samples were then washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 300xg and the supernatant removed. The washing step was repeated twice. The washed cells were resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flowcytometer.

Staining with dextramers on samples from HLA-matched and CMV-infected individuals resulted in identification of CD8 positive T cells specific for the respectively Dextramer and thereby the presence of CMV specific T cells, while no MHC Dextramer positive staining was observed with the negative control Dextramer carrying the nonsense- peptide AAKGRGAAL (B^*0801 -cys- AAKGRGAAL/RPE). Results are shown in figures 29-31 .

Staining with Dextramers on samples from HLA-mismatched donors (I.e. donors not carrying the A^*0101 and/or B^*0702 allele resulted in no MHC Dextramer positive staining of CD8 positive cells (data not shown). This example demonstrates that Dextramers can be used for identification of CMV specific CD8 positive T cells if the Dextramer carries a CMV derived antigenic peptide in the peptide binding cleft.

Example 52 This is an example of generation of MHC multimers where the multimerization domain is Dextran with Streptavidin (SA) covalently attached. Chemically biotinylated MHC- peptide molecules were coupled to the Dextran-SA multimerization domain through non-covalent interactions between biotin and SA. The dextran multimerization domain also had fluorochrome molecules covalently attached. The MHC-peptide molecules were: A^*0301 molecules binding the CMV derived antigenic peptide KLGGALQAK, A^*2402 molecules binding the CMV derived antigenic peptide VYALPLKML, A^*2402 molecules binding the CMV derived antigenic peptide QYDPVAALF, B^*3501 molecules binding the CMV derived antigenic peptide IPSINVHHY or B^*0801 molecules binding the nonsense peptide AAKGRGAAL in the peptide binding groove.

The flurochrome used was PE.

Chemically biotinylated MHC-peptide molecules were generated as follows:

Recombinant Heavy chain molecules with a C-terminal Cysteine residue were made by generation of a DNA expression vector encoding the Heavy chain molecule (without the intracellular and transmembrane domains) fused to the sequence GSLHHILDAQKMVWNHRCG. This expression vector was transformed into BL21 DE3 bacteria. Likewise a DNA expression vector encoding human beta2microglobulin (β2m) were generated and transformed into BL21 DE3 bacteria. β2m and the Heavy chain- Cys fusion protein were expressed and purified from inclusion bodies using standard procedures as described in Garboczi et al. (1996), Nature 384, 134-141. MHC-peptide complexes was generated by in vitro refolding of Heavy chain-cys fusion protein, β2m and either of the peptides in a buffer containing reduced and oxidized gluthatione using the following steps:

1 . 100 ml of refolding buffer (10O mM Tris, 400 mM L-arginin-HCL, 2 mM NaEDTA, 0.5 mM oxidized Gluthathione, 5 mM reduced Glutathione, pH 8.0) was supplied with protease inhibitors PMSF (phenylmethylsulphonyl fluoride), Pepstatin A and Leupeptin (to a final concentration of 1 mM, 1 mg/l and 1 mg/l, respectively). The refolding buffer was placed at 10 ⁰C.

2. 6 mg of peptide was dissolved in DMSO (200-300 μl), and added drop-wise to the refolding buffer at vigorous stirring. 3. 6,6 mg of β2m was added drop-wise to the refolding buffer at vigorous stirring.

7. Correctly folded MHC-complex was separated and purified from excess β2m, excesss heavy chain and aggregates thereoff, by size exclusion chromatography on a column that separates proteins in the 10-100 kDa range. Correctly folded monomer MHC-complex was eluted with a MHC-buffer (20 mM Tris-HCI, 50 mM NaCI, pH 8.0).

8. Fractions containing the folded monomer MHC-peptide complex were pooled, the buffer exchanged to PBS, pH = 7.0 and the sample concentrated to approximately 1 ml in a suitable concentrator with a 5000 mw cut-off filter. The protein-concentration was estimated from its absorption at 280 nm.

Correct foldning of purified MHC-cys-peptide molecules were verified as described in example 34. Then the molecules were chemically biotinylated by treating purified MHC- cys-peptide molecules with Maleimide-biotin (EZ-link Maleimide-PEO2-Biotin). The reaction was carried out in PBS, PH 7.0 for 2¹/2 hour at 4°C, with a Maleimide-biotin: MHC-cys-peptide molecule molar ratio equal to 1.5 (1 ,6 ug Maleimide-biotin per 100 ug MHC-cys-peptide molecules). Excess Maleimide-biotin was removed and the buffer exchanged to 20 mM Tris-HCI, 50 mM NaCI, pH 8.0 by gel filtration in a G-25 column. Efficiency of biotinylation was determined using a SDS PAGE shift assay with SA. 1 ug Maleimide-biotin treated MHC-cys-peptide molecules was incubated with 1 .8 ug SA in 12 ul PBS, pH 7.0 for 1 hour at room temperature. Then the sample was analyzed by SDS PAGE together with samples of various concentrations of Maleimide-biotin treated MHC-cys-peptide molecules not incubated with SA and a sample with SA alone. The degree of biotinylation was estimated to approximately 70-90%. Biotinylated MHC-cys-peptide molecules were then coupled to PE-SA-conjugated 27OkDA dextran in a molar ratio of MHC-peptide complexes: SA equal to 3: 1. The PE- SA-conjugated 27OkDA dextran was generated as described in example 6 and 7 herein and contained 5.4 mol SA and 3,3 mol PE per mol dextran. The coupling of biotinylated MHC-cys-peptide molecules to PE-SA-conjugated 27OkDA dextran were done by mixing biotinylated MHC-cys-peptide molecules with PE-SA-conjugated 27OkDA dextran (16x10⁸ M) followed by incubation for 30 minutes at room temperature in the dark. Then buffer (0.05M Tris-HCI, 15mM NaN₃, 1% BSA, pH 7.2) was added. O

A^*0301 (KLGGALQAK) 62,2 ul (0,63 mg/ml) + 315 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1575 ul.

A^*2402 (VYALPLKML) 68,2 ul (0,43 mg/ml) + 231 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1 155 ul. A^*2402 (QYDPVAALF) 94,3 ul (0,31 mg/ml) + 231 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1 155 ul.

B^*3501 (IPSINVHHY) 99,1 ul (0,27 mg/ml) + 210 ul -SA-conjugated 27OkDA dextran. Buffer to a final volume of 1050 ul.

The binding of the above described MHC-cys-peptide molecules /PE dextran complexes were used to determine the presence of CMV IE or pp65 specific T cells in the blood from HLA-matched CMV infected individual by flow cytometry following a Standard flow cytometry protocol.

Briefly, blood from CMV infected individuals was withdrawn and 200-400 ul of blood incubated with 10 ul of either of the above described dextramer constructs or a negative control Dextramer (B^*0801 -cys- AAKGRGAAL/RPE , generated as described in example 49) for 10-30 minutes at room temperature (18-25 °C) in the dark . 5-10 μl of each of each of the antibodies mouse-anti-human CD3/PCP and mouse-anti-human CD8/FITC were added and the incubation continued for another 20-30 minutes at 4⁰C in the dark. 100 ul UTI-Lyse reagent A was added to each tube, incubation for 10 minutes at roomtemperature in the dark. 1 ml UTI-Lyse reagent B was added to each and incubation continued for 10 minutes at room temperature in the dark. Samples were centrifuged for 5 minutes at 300xg, supernatant removed, cells resuspended in 2 ml PBS; pH =7.0 followed by centrifugation for 5 minutes at 300xg and the supernatant removed. The washed cells were resuspended in 400-500 μl PBS + 2% Formaldehyde and analyzed on a flowcytometer.

Staining with dextramers on samples from HLA-matched and CMV infected individuals resulted in identification of 0,1 - 20% CD8 positive T cells specific for the respective Dextramers and thereby the presence of CMV specific T cells, while no MHC Dextramer positive staining was observed with the negative control Dextramer carrying the nonsense-peptide AAKGRGAAL (B^*0801 -cys- AAKGRGAAL/RPE) (data not shown). Staining with Dextramers on samples from HLA-mismatched donors resulted in no MHC Dextramer positive staining of CD8 positive cells. This example demonstrates that Dextramers can be used for identification of CMV specific CD8 positive T cells if the Dextramer carries a CMV derived antigenic peptide in the peptide binding cleft. o o

Example 53

This is an example of generation of MHC multimers where the multimerization domain is Dextran with Streptavidin (SA) covalently attached. Chemically biotinylated MHC- peptide molecules are coupled to the Dextran-SA multimerization domain through non- covalent interactions between biotin and SA. The dextran multimerization domain also has fluorochrome molecules covalently attached.

Chemically biotinylated MHC-peptide molecules are generated as described within example 49. MHC-peptide complexes are generated by in vitro refolding of Heavy chain-cys fusion protein, β2m and either of the peptides from CMV IE, pp50 or pp65. Correct foldning of purified MHC-cys-peptide molecules are verified as described in example 34. Then the molecules are chemically biotinylated by treating purified MHC- cys-peptide molecules with Maleimide-biotin (EZ-link Maleimide-PEO2-Biotin). The reaction is carried out in PBS, PH 7.0 for 2Vz hour at 4°C, with a Maleimide-biotin:

MHC-cys-peptide molecule molar ratio equal to 1.5 (1 ,6 ug Maleimide-biotin per 100 ug MHC-cys-peptide molecules). Excess Maleimide-biotin was removed and the buffer exchanged to 20 mM Tris-HCI, 50 mM NaCI, pH 8.0 by gel filtration in a G-25 column. Efficiency of biotinylation is determined using a SDS PAGE shift assay with SA. 1 ug Maleimide-biotin treated MHC-cys-peptide molecules is incubated with 1.8 ug SA in 12 ul PBS, pH 7.0 for 1 hour at room temperature. Then the sample is analyzed by SDS PAGE together with samples of various concentrations of Maleimide-biotin treated MHC-cys-peptide molecules not incubated with SA and a sample with SA alone. The degree of biotinylation is estimated. Biotinylated MHC-cys-peptide molecules are then coupled to PE-SA-conjugated 27OkDA dextran in a molar ratio of MHC-peptide complexes: SA equal to 3: 1. The coupling of biotinylated MHC-cys-peptide molecules to PE-SA-conjugated 27OkDA dextran are done by mixing biotinylated MHC-cys-peptide molecules with PE-SA- conjugated 27OkDA dextran (16x10⁸ M) followed by incubation for 30 minutes at room temperature in the dark. Then buffer (0.05M Tris-HCI, 15mM NaN₃, 1 % BSA, pH 7.2) was added.

The binding of the above described MHC-cys-peptide molecules /PE dextran complexes are used to determine the presence of CMV IE, pp50 or pp65 specific T cells in the blood from HLA-matched CMV infected individuals by flow cytometry following a standard flow cytometry protocol for staining on whole blood (e.g. as described in example 51 (a lyse procedure) or in example 28 (a no-lyse procedure) ) or a standard procedure for analysis of purified HPBMCs as described in the following. Briefly, blood from a donor/individual is withdrawn and HPBMC isolated by ficoll- Hypaque purification using a Standard procedure. Approximately 1x10⁶ purified HPBMC at a concentration of 2x10⁷cells/ml are incubated with 10 ul dextramer constructs for 10 minutes at 4⁹C in the dark at room temperature. 5 μl of each of each of the antibodies mouse-anti-human CD3/APC (clone UCHT1 from Dako), mouse-anti- human CD4/FITC (clone MT310 from Dako) and mouse-anti-human CD8/PB (clone DK25 from Dako) are added and the incubation continues for another 20 minutes at 4⁰C in the dark. The samples are washed by adding 2 ml PBS; pH =7.2 followed by centrifugation for 5 minutes at 300xg and the supernatant removed. The washing step is repeated twice. The washed cells are resuspended in 400-500 μl PBS; pH=7.2 and analyzed on a flowcytometer. Staining with dextramers will resulte in identification of CD8 positive T cells specific for the respectively Dextramer and thereby the presence of CMV specific T cells, while no MHC Dextramer positive staining is expected with a negative control Dextramer e.g. carrying a nonsense-peptide or another irrelevant peptide.

Staining with Dextramers on samples from HLA-mismatched donors results in no MHC Dextramer positive staining of CD8 positive cells. This example will demonstrate that Dextramers may be used for identification of CMV specific CD8 positive T cells if the Dextramer carries a CMV derived antigenic peptide in the peptide binding cleft.

Example 54 This is an example of generation of MHC multimers where the multimerization domain is Dextran with Streptavidin (SA) covalently attached. Chemically biotinylated MHC- peptide molecules are coupled to the Dextran-SA multimerization domain through non- covalent interactions between biotin and SA. The dextran multimerization domain also has fluorochrome molecules covalently attached. Chemically biotinylated MHC-peptide molecules are generated as described within example 49.

MHC-peptide complexes are generated by in vitro refolding of Heavy chain-cys fusion protein, β2m and either of the peptides from CMV IE, pp50 or pp65. Correct foldning of purified MHC-cys-peptide molecules are verified as described in example 34. Then the molecules are chemically biotinylated by treating purified MHC- cys-peptide molecules with Maleimide-biotin (EZ-link Maleimide-PEO2-Biotin) or another derivatised biotin molecule capable of reacting with an -SH group on a cystein residue. The reaction is carried out in a buffer with, PH 6.0-8.5 for ¹/2-24 hours at 4°C, with a biotin: MHC-cys-peptide molecule molar ratio between 1 -10. Excess biotin are removed and the buffer exchanged to 20 mM Tris-HCI, 50 mM NaCI, pH 8.0 or another buffer e.g. PBS with pH between 6-8,5 by gel filtration in a G-25 column or another suitable column able to separate biotin from MHC-cys-peptide molecules (e.g. a G-75 or G-200 column). Efficiency of biotinylation is determined using a SDS PAGE shift assay with SA. 0.1 -20 ug Maleimide-biotin treated MHC-cys-peptide molecules is incubated with 0,1 -50 ug SA in a suitable volume of PBS, pH 6.0-9 for 0.1 -5 hours at room temperature. Then the sample is analyzed by SDS PAGE together with samples of various concentrations of Maleimide-biotin treated MHC-cys-peptide molecules not incubated with SA and a sample with SA alone. The degree of biotinylation is estimated. Biotinylated MHC-cys-peptide molecules are then coupled to PE-SA-conjugated

27OkDA dextran in a molar ratio of MHC-peptide complexes: SA equal to 3: 1 or equal to 2:1 or 1 :1 or 1 :2 or 1 :2 or 1 :3. The coupling of biotinylated MHC-cys-peptide molecules to PE-SA-conjugated 27OkDA dextran are done by mixing biotinylated MHC- cys-peptide molecules with PE-SA-conjugated 27OkDA dextran (16x10⁸ M) followed by incubation for 10-300 minutes at room temperature in the dark. Then buffer (e.g. 0.05M Tris-HCI, 15mM NaN₃, 1 % BSA, pH 7.2 or another Tris-, PBS or other suitable buffer with pH &.0-9.0) is added.

The binding of the above described MHC-cys-peptide molecules /PE dextran complexes are used to determine the presence of CMV IE, pp50 or pp65 specific T cells in the blood from HLA-matched CMV infected individual by flow cytometry following a standard flow cytometry protocol for staining on whole blood (either with or without lysis of red blood cells and woth or without washing) or a standard procedure for analysis of purified HPBMCs as described in the following.. Blood from CMV infected individual is withdrawn and HPBMC isolated by ficoll-Hypaque purification using a standard procedure. 1 x10⁵- 1x10⁷ purified HPBMC at a concentration of 1x10⁶- 1x10⁸ cells/ml are incubated with 2-20 ul dextramer constructs for 5-60 minutes at 4- 30° C in the dark at room temperature. Each of the antibodies mouse-anti-human CD3/APC, mouse-anti-human CD4/FITC and mouse-anti-human CD8/PB are added in a concentration and volume as recommended by the amnufacturer and the incubation continues for another 5-100 minutes at 4-3O⁰C in the dark. The samples are washed by adding 2 ml PBS; pH =7.2 or another suitable buffer with pH between 6.0-9.0 followed by centrifugation for 2-60 minutes at 100-1000xg and the supernatant removed. The washing step is optionally repeated. The washed cells are resuspended in 200-2000 μl PBS; pH=7.2 or another suitable buffer with pH 6.0-9.0 and analyzed on a flowcytometer.

Staining with dextramers will result in identification of CD8 positive T cells specific for the respectively Dextramer and thereby the presence of CMV specific T cells. Staining with Dextramers on samples from HLA-mismatched donors will result in no MHC Dextramer positive staining of CD8 positive cells. This example demonstrates that Dextramers may be used for identification of CMV specific CD8 positive T cells if the Dextramer carries a CMV derived antigenic peptide in the peptide binding cleft.

Claims

1. A MHC monomer comprising a-b-P, or a MHC multimer comprising (a-b-P)_n, wherein n > 1 ,

wherein (a-b-P) is the MHC-peptide complex formed when the peptide P binds to the functional MHC protein,

2. The MHC multimer according to claim 1 , wherein the association is a covalent linkage so that one or more of the n MHC-peptide complexes is covalently linked to the one or more multimerization domains.

3. The MHC multimer according to claim 1 , wherein the association is a non- covalent association so that one or more of the n MHC-peptide complexes is non- covalently associated with the one or more multimerization domains.

4. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more scaffolds.

5. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more carriers.

6. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises at least one scaffold and at least one carrier.

7. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprise one or more optionally substituted organic molecules. £-

8. The MHC multimer according to claim 7, wherein the optionally substituted organic molecule comprises one or more functionalized cyclic structures.

9. The MHC multimer according to claim 8, wherein the one or more functionalized cyclic structures comprises one or more benzene rings.

10. The MHC multimer according to claim 7, wherein the optionally substituted organic molecule comprises a scaffold molecule comprising at least three reactive groups, or at least three sites suitable for non-covalent attachment.

1 1 . The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more biological cells and/or cell-like structures, such as antigen presenting cells or dendritic cells.

12. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more membranes.

13. The MHC multimer according to claim 12, wherein the one or more membranes comprises liposomes or micelles.

14. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more polymers such as one or more synthetic polymers.

15. The MHC multimer according to claim 14, wherein the one or more polymers are selected from the group consisting of polysaccharides.

16. The MHC multimer according to claim 15, wherein the polysaccharide comprises one or more dextran moieties.

17. The MHC multimer according to claim 16, wherein the one or more dextran moieties are covalently attached to one or more MHC peptide complexes.

18. The MHC multimer according to claim 16, wherein the one or more dextran moieties are non-covalently attached to one or more MHC peptide complexes.

19. The MHC multimer according to claim 16, wherein the one or more dextran moieties are modified.

20. The MHC multimer according to claim 16, wherein the one or more dextran moieties comprises one or more Amino-dextrans.

21 . The MHC multimer according to claim 16, wherein the one or more dextran moieties comprises one or more Amino-dextrans modified with divinyl sulfone.

22. The MHC multimer according to claim 16, wherein the one or more dextran moieties comprises one or more dextrans with a molecular weight of from 1 ,000 to 50,000, such as from 1 ,000 to 5,000, for example 5,000 to 10,000, such as from 10,000 to 15,000, for example 15,000 to 20,000, such as from 20,000 to 25,000, for example 25,000 to 30,000, such as from 30,000 to 35,000, for example 35,000 to 40,000, such as from 40,000 to 45,000, for example 45,000 to 50,000, including any consecutive combination of the afore-mentioned ranges.

23. The MHC multimer according to claim 16, wherein the one or more dextran moieties comprises one or more dextrans with a molecular weight of from 50,000 to 150,000, such as from 50,000 to 60,000, for example 60,000 to 70,000, such as from

70,000 to 80,000, for example 80,000 to 90,000, such as from 90,000 to 100,000, for example 100,000 to 1 10,000, such as from 1 10,000 to 120,000, for example 120,000 to 130,000, such as from 130,000 to 140,000, for example 140,000 to 150,000, including any consecutive combination of the afore-mentioned ranges.

24. The MHC multimer according to claim 16, wherein the one or more dextran moieties comprises one or more dextrans with a molecular weight of from 150,000- 270,000 such as from 150,000 to 160,000, for example 160,000 to 170,000, such as from 170,000 to 180,000, for example 180,000 to 190,000, such as from 190,000 to 200,000, for example 200,000 to 210,000, such as from 210,000 to 220,000, for example 220,000 to 230,000, such as from 230,000 to 240,000, for example 240,000 to 250,000, such as from 250,000 to 260,000, for example 260,000 to 270,000, such as from 270,000 to 280,000, for example 280,000 to 290,000, such as from 290,000 to 300,000, for example 300,000 to 310,000 such as from 310,000 to 320,000, for example 320,000 to 330,000 such as from 330,000 to 340,000, for example 340,000 to 350,000 such as from 350,000 to 360,000, for example 360,000 to 370,000 such as from 370,000 to 380,000, for example 380,000 to 390,000, such as from 390,000 to 400,000, including any consecutive combination of the afore-mentioned ranges.

25. The MHC multimer according to claim 16, wherein the one or more dextran moieties are linear.

26. The MHC multimer according to claim 16, wherein the one or more dextran moieties are branched.

27. The MHC multimer according to claim 14, wherein the one or more synthetic polymers are selected from the group consisting of PNA, polyamide and PEG.

28. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more entities selected from the group consisting of an IgG domain, a coiled-coil polypeptide structure, a DNA duplex, a nucleic acid duplex, PNA-PNA, PNA-DNA, DNA-RNA.

29. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises one or more avidins, such as one or more streptavidins.

30. The MHC multimer according to claim 29, wherein the one or more streptavidins comprises one or more tetrameric streptavidin variants.

31 . The MHC multimer according to claim 29, wherein the one or more streptavidins comprises one or more monomeric streptavidin variants.

32. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises an antibody.

33. The MHC multimer according to claim 32, wherein the antibody is selected from the group consisting of polyclonal antibody, monoclonal antibody, IgA, IgG, IgM, IgD, IgE, IgGI , lgG2, lgG3, lgG4, IgAI , lgA2, IgMI , lgM2, humanized antibody, humanized monoclonal antibody, chimeric antibody, mouse antibody, rat antibody, rabbit antibody, human antibody, camel antibody, sheep antibody, engineered human antibody, epitope-focused antibody, agonist antibody, antagonist antibody, neutralizing antibody, naturally-occurring antibody, isolated antibody, monovalent antibody, bispecific antibody, trispecific antibody, multispecific antibody, heteroconjugate antibody, immunoconjugates, immunoliposomes, labeled antibody, antibody fragment, domain antibody, nanobody, minibody, maxibody, diabody, fusion antibody.

34. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more small organic scaffold molecules.

35. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more small organic molecules.

36. The MHC multimer according to claim 35, wherein the one or more small organic molecules comprises one or more steroids.

37. The MHC multimer according to claim 35, wherein the one or more small organic molecules comprises one or more peptides.

38. The MHC multimer according to claim 35, wherein the one or more small organic molecules comprises one or more aromatic organic molecules.

39. The MHC multimer according to claim 38, wherein the one or more aromatic organic molecules comprises one or more monocyclic structures.

40. The MHC multimer according to claim 39, wherein the one or more monocyclic structures comprises one or more optionally functionalized or substituted benzene rings.

41. The MHC multimer according to claim 38, wherein the one or more aromatic organic molecules comprises one or more dicyclic structures.

42. The MHC multimer according to claim 38, wherein the one or more aromatic organic molecules comprises one or more polycyclic structures.

43. The MHC multimer according to claim 35, wherein the one or more small organic molecules comprises one or more aliphatic molecules.

44. The MHC multimer according to claim 43, wherein the one or more aliphatic molecules comprises one or more monocyclic molecules.

45. The MHC multimer according to claim 43, wherein the one or more aliphatic molecules comprises one or more dicyclic molecules.

46. The MHC multimer according to claim 43, wherein the one or more aliphatic molecules comprises one or more polycyclic molecules.

47. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more monomeric molecules able to polymerize.

48. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more biological polymers such as one or more proteins.

49. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more small molecule scaffolds.

50. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more supramolecular structure(s) such as one or more nanoclusters.

51. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more further polypeptides in addition to a and b.

52. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more protein complexes.

53. The MHC multimer according to claim 1 , wherein the MHC multimer comprises one or more beads.

54. The MHC multimer according to claim 53, wherein the one or more beads can be selected from the groups consisting of beads that carry electrophilic groups e.g. O--O

divinyl sulfone activated polysaccharide, polystyrene beads that have been functionalized with tosyl-activated esters, magnetic polystyrene beads functionalized with tosyl-activated esters, and beads where MHC complexes have been covalently immobilized to these by reaction of nucleophiles comprised within the MHC complex with the electrophiles of the beads.

55. The MHC multimer according to claim 53, wherein the one or more beads can be selected from the groups consisting of sepharose beads, sephacryl beads, polystyrene beads, agarose beads, polysaccharide beads, polycarbamate beads and any other kind of beads that can be suspended in an aqueous buffer.

56. The MHC multimer according to claim 1 , wherein the multimerization domain comprises one or more compounds selected from the group consisting of agarose, sepharose, resin beads, glass beads, pore-glass beads, glass particles coated with a hydrophobic polymer, chitosan-coated beads, SH beads, latex beads , spherical latex beads, allele-type beads, SPA bead, PEG-based resins, PEG-coated bead, PEG- encapsulated bead, polystyrene beads, magnetic polystyrene beads, glutathione agarose beads, magnetic bead, paramagnetic beads, protein A and/or protein G sepharose beads, activated carboxylic acid bead, macroscopic beads, microscopic beads, insoluble resin beads, silica-based resins, cellulosic resins, cross-linked agarose beads, polystyrene beads, cross-linked polyacrylamide resins, beads with iron cores, metal beads, dynabeads, Polymethylmethacrylate beads activated with NHS, streptavidin-agarose beads, polypropylene, polyethylene, dextran, nylon, amylases, natural and modified celluloses, nitrocellulose, polyacrylamides, gabbros, magnetite, polymers, oligomers, non-repeating moieties, polyethylene glycol (PEG), monomethoxy-PEG, mono-(Ci-Ci₀)alkoxy-PEG, aryloxy-PEG, poly-(N-vinyl pyrrolidone)PEG, tresyl monomethoxy PEG, PEG propionaldehyde, bis-succinimidyl carbonate PEG, polystyrene bead crosslinked with divinylbenzene, propylene glycol homopolymers, a polypropylene oxide/ethylene oxide co-polymer, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, dextran, aminodextran, carbohydrate-based polymers, cross-linked dextran beads, polysaccharide beads, polycarbamate beads, divinyl sulfone activated polysaccharide, polystyrene beads that have been functionalized with tosyl-activated esters, magnetic polystyrene beads functionalized with tosyl-activated esters, streptavidin beads, streptaivdin-monomer coated beads, streptaivdin-tetramer coated beads, Streptavidin Coated Compel Magnetic beads, avidin coated beads, dextramer coated beads, divinyl sulfone-activated dextran, Carboxylate-modified bead, amine-modified beads, antibody coated beads, cellulose beads, grafted co-poly beads, poly-acrylamide beads, dimethylacrylamide beads optionally crosslinked with N-N'-bis-acryloylethylenediamine, hollow fiber membranes, fluorescent beads, collagen-agarose beads, gelatin beads, collagen- gelatin beads, collagen-fibronectin-gelatin beads, collagen beads, chitosan beads, collagen-chitosan beads, protein-based beads, hydrogel beads, hemicellulose, alkyl cellulose, hydroxyalkyl cellulose, carboxymethylcellulose, sulfoethylcellulose, starch, xylan, amylopectine, chondroitin, hyarulonate, heparin, guar, xanthan, mannan, galactomannan, chitin and chitosan.

57. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a dimerization domain.

58. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a trimerization domain.

59. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a tetramerization domain.

60. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a pentamerization domain.

61 . The MHC multimer according to claim 60, wherein the pentamerization domain comprises a coiled-coil polypeptide structure.

62. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a hexamerization domain.

63. The MHC multimer according to claim 62, wherein the hexamerization domain comprises three IgG domains.

64. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a polymer structure to which is attached one or more scaffolds. oo

65. The MHC multimer according to claim 64, wherein the polymer structure comprises a polysaccharide.

66. The MHC multimer according to claim 65, wherein the polysaccharide comprises one or more dextran moieties.

67. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a polyamide and/or a polyethylene glycol and/or a polysaccharide and/or a sepharose.

68. The MHC multimer according to claim 1 , wherein the one or more multimerization domains comprises a carboxy methyl dextran and/or a dextran polyaldehyde and/or a carboxymethyl dextran lactone and/or a cyclodextrin.

69. The MHC multimer according to claim 1 , wherein the one or more multimerization domains have a molecular weight of less than 1 ,000 Da.

70. The MHC multimer according to claim 1 , wherein the one or more multimerization domains have a molecular weight of from 1 ,000 Da to preferably less than 10,000 Da.

71 . The MHC multimer according to claim 1 , wherein the one or more multimerization domains have a molecular weight of from 10,000 Da to preferably less than 100,000 Da.

72. The MHC multimer according to claim 1 , wherein the one or more multimerization domains have a molecular weight of from 100,000 Da to preferably less than 1 ,000,000 Da.

73. The MHC multimer according to claim 1 , wherein the one or more multimerization domains have a molecular weight of more than 1 ,000,000 Da.

74. The MHC multimer according to claim 1 , wherein the one or more multimerization domains have a molecular weight of from 50,000 Da to preferably less than 1 ,000,000 Da, such as from 50,000 Da to 980,000; for example from 50,000 Da to UU

960,000; such as from 50,000 Da to 940,000; for example from 50,000 Da to 920,000; such as from 50,000 Da to 900,000; for example from 50,000 Da to 880,000; such as from 50,000 Da to 860,000; for example from 50,000 Da to 840,000; such as from 50,000 Da to 820,000; for example from 50,000 Da to 800,000; such as from 50,000 Da to 780,000; for example from 50,000 Da to

760,000; such as from 50,000 Da to 740,000; for example from 50,000 Da to 720,000; such as from 50,000 Da to 700,000; for example from 50,000 Da to 680,000; such as from 50,000 Da to 660,000; for example from 50,000 Da to 640,000; such as from 50,000 Da to 620,000; for example from 50,000 Da to 600,000; such as from 50,000 Da to 580,000; for example from 50,000 Da to

560,000; such as from 50,000 Da to 540,000; for example from 50,000 Da to 520,000; such as from 50,000 Da to 500,000; for example from 50,000 Da to 480,000; such as from 50,000 Da to 460,000; for example from 50,000 Da to 440,000; such as from 50,000 Da to 420,000; for example from 50,000 Da to 400,000; such as from 50,000 Da to 380,000; for example from 50,000 Da to

360,000; such as from 50,000 Da to 340,000; for example from 50,000 Da to 320,000; such as from 50,000 Da to 300,000; for example from 50,000 Da to 280,000; such as from 50,000 Da to 260,000; for example from 50,000 Da to 240,000; such as from 50,000 Da to 220,000; for example from 50,000 Da to 200,000; such as from 50,000 Da to 180,000; for example from 50,000 Da to

160,000; such as from 50,000 Da to 140,000; for example from 50,000 Da to 120,000; such as from 50,000 Da to 100,000; for example from 50,000 Da to 80,000; such as from 50,000 Da to 60,000; for example from 100,000 Da to 1 ,000,000; such as from 100,000 Da to 980,000; for example from 100,000 Da to 960,000; such as from 100,000 Da to 940,000; for example from 100,000 Da to 920,000; such as from

100,000 Da to 900,000; for example from 100,000 Da to 880,000; such as from 100,000 Da to 860,000; for example from 100,000 Da to 840,000; such as from 100,000 Da to 820,000; for example from 100,000 Da to 800,000; such as from 100,000 Da to 780,000; for example from 100,000 Da to 760,000; such as from 100,000 Da to 740,000; for example from 100,000 Da to 720,000; such as from

100,000 Da to 700,000; for example from 100,000 Da to 680,000; such as from 100,000 Da to 660,000; for example from 100,000 Da to 640,000; such as from 100,000 Da to 620,000; for example from 100,000 Da to 600,000; such as from 100,000 Da to 580,000; for example from 100,000 Da to 560,000; such as from 100,000 Da to 540,000; for example from 100,000 Da to 520,000; such as from t—

100,000 Da to 500,000; for example from 100,000 Da to 480,000; such as from 100,000 Da to 460,000; for example from 100,000 Da to 440,000; such as from 100,000 Da to 420,000; for example from 100,000 Da to 400,000; such as from 100,000 Da to 380,000; for example from 100,000 Da to 360,000; such as from 100,000 Da to 340,000; for example from 100,000 Da to 320,000; such as from

100,000 Da to 300,000; for example from 100,000 Da to 280,000; such as from 100,000 Da to 260,000; for example from 100,000 Da to 240,000; such as from 100,000 Da to 220,000; for example from 100,000 Da to 200,000; such as from 100,000 Da to 180,000; for example from 100,000 Da to 160,000; such as from 100,000 Da to 140,000; for example from 100,000 Da to 120,000; such as from

150,000 Da to 1 ,000,000; for example from 150,000 Da to 960,000; such as from 150,000 Da to 940,000; for example from 150,000 Da to 920,000; such as from 150,000 Da to 900,000; for example from 150,000 Da to 880,000; such as from 150,000 Da to 860,000; for example from 150,000 Da to 840,000; such as from 150,000 Da to 820,000; for example from 150,000 Da to 800,000; such as from

150,000 Da to 780,000; for example from 150,000 Da to 760,000; such as from 150,000 Da to 740,000; for example from 150,000 Da to 720,000; such as from 150,000 Da to 700,000; for example from 150,000 Da to 680,000; such as from 150,000 Da to 660,000; for example from 150,000 Da to 640,000; such as from 150,000 Da to 620,000; for example from 150,000 Da to 600,000; such as from

150,000 Da to 580,000; for example from 150,000 Da to 560,000; such as from 150,000 Da to 540,000; for example from 150,000 Da to 520,000; such as from 150,000 Da to 500,000; for example from 150,000 Da to 480,000; such as from 150,000 Da to 460,000; for example from 150,000 Da to 440,000; such as from 150,000 Da to 420,000; for example from 150,000 Da to 400,000; such as from

150,000 Da to 380,000; for example from 150,000 Da to 360,000; such as from 150,000 Da to 340,000; for example from 150,000 Da to 320,000; such as from 150,000 Da to 300,000; for example from 150,000 Da to 280,000; such as from 150,000 Da to 260,000; for example from 150,000 Da to 240,000; such as from 150,000 Da to 220,000; for example from 150,000 Da to 200,000; such as from

150,000 Da to 180,000; for example from 150,000 Da to 160,000.

75. The MHC multimer according to claim 1 further comprising one or more scaffolds, carriers and/or linkers selected from the group consisting of streptavidin (SA) and avidin and derivatives thereof, biotin, immunoglobulins, antibodies (monoclonal, polyclonal, and recombinant), antibody fragments and derivatives thereof, leucine zipper domain of AP-1 (jun and fos), hexa-his (metal chelate moiety), hexa-hat GST (glutathione S-tranferase) glutathione affinity, Calmodulin-binding peptide (CBP), Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, E2Tag, HA Epitope Tag, Myc

Epitope, FLAG Epitope, AU1 and AU5 Epitopes, GIu-GIu Epitope, KT3 Epitope, IRS Epitope, Btag Epitope, Protein Kinase-C Epitope, VSV Epitope, lectins that mediate binding to a diversity of compounds, including carbohydrates, lipids and proteins, e.g. Con A (Canavalia ensiformis) or WGA (wheat germ agglutinin) and tetranectin or Protein A or G (antibody affinity).

76. The MHC multimer according to claim 1 , wherein n is 2.

77. The MHC multimer according to claim 1 , wherein n is 3.

78. The MHC multimer according to claim 1 , wherein n is 4.

79. The MHC multimer according to claim 1 , wherein n is 5.

80. The MHC multimer according to claim 1 , wherein n is 6.

81 . The MHC multimer according to claim 1 , wherein n is 7.

82. The MHC multimer according to claim 1 , wherein n is 8.

83. The MHC multimer according to claim 1 , wherein n is 9.

84. The MHC multimer according to claim 1 , wherein n is 10.

85. The MHC multimer according to claim 1 , wherein n is 1 1.

86. The MHC multimer according to claim 1 , wherein n is 12.

87. The MHC multimer according to claim 1 , wherein n ≥ 13.

88. The MHC multimer according to claim 1 , wherein 1 < n ≥ 100.

89. The MHC multimer according to claim 1 , wherein 1 < n ≥ 1000.

90. The MHC multimer according to claim 1 , wherein n ≥ 1 ,000,000,000.

91 . The MHC multimer according to claim 1 , wherein n ≥ 1 ,000,000,000,000,000,000 (one trillion).

92. The MHC multimer according to any of claims 1 to 91 , wherein n is selected from the group of integers consisting of 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, 30, 31 , 32, 33, 34, 35, 36, 37, 38, 39, 40, 41 , 42, 43, 44, 45, 46, 47, 48, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 1 10, 120, 130, 140, 150, 160, 170, 180, 190, 200, 250, 300, 350, 400, 450, 500, 550, 600, 650, 700, 750, 800, 850, 900 and 1000.

93. The MHC multimer according to any of claims 1 to 91 , wherein n has a value of from 1 to 1000, for example from 1 to 10, such as from 10 to 20, for example from 20 to 30, such as from 30 to 40, for example from 40 to 50, such as from 50 to 60, for example from 60 to 70, such as from 70 to 80, for example from 80 to 90, such as from 90 to 100, for example from 100 to 120, such as from 120 to 140, for example from 140 to 160, such as from 160 to 180, for example from 180 to 200, such as from 200 to 250, for example from 250 to 300, such as from 300 to 350, for example from 350 to 400, such as from 400 to 450, for example from 450 to 500, such as from 500 to 550, for example from 550 to 600, such as from 600 to 650, for example from 650 to 700, such as from 700 to 750, for example from 750 to 800, such as from 800 to 900, for example from 900 to 950, such as from 950 to 1000, including any consecutive combinations of the aforementioned ranges.

94. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of human origin.

95. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of mouse origin.

96. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of primate origin.

97. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of chimpansee origin.

98. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of gorilla origin.

99. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of orangutan origin.

100. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of monkey origin.

101. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Macaque origin.

102. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of porcine (swine/pig) origin.

103. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of bovine (cattle/antilopes) origin.

104. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of equine (horse) origin.

105. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Camelides (camels) origin.

106. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of ruminant origin.

107. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Canine (Dog) origin.

108. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of

Feline (Cat) origin.

109. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of

Bird origin.

1 10. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Chicken origin.

1 1 1. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Turkey origin.

1 12. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Fish origin.

1 13. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of Reptile origin.

1 14. The MHC multimer according to claim 1 , wherein a and/or b and/or P is of

Amphibian origin.

1 15. The MHC multimer according to any of claims 1 to 1 14, wherein (a-b-P) is a class 1 MHC-peptide complex.

1 16. The MHC multimer according to any of claims 1 to 1 14, wherein (a-b-P) is a class 2 MHC-peptide complex.

1 17. The MHC multimer according to any of claims 1 to 1 16, wherein the MHC protein is the same or different MHC proteins selected from the group of HLA classes consisting of

A^*01010101 B^*070201 Cw^*010201 E^*01010101 F^*01010101 G^*01010101 , A^*01010102NB^*070202Cw^*010202E^*01010102F^*01010102G^*01010102, A^*010102B^*070203Cw^*010203E^*01010103F^*01010103G^*01010103, A^*010103B^*070204Cw^*010204E^*01030101 F^*01010104G^*01010104, A^*010104B^*0703Cw^*0103E^*01030102F^*01010105G^*01010105, A^*0102B^*0704Cw^*0104E^*010302F^*01010106G^*01010201 , A^*0103B^*070501 Cw^*0105E^*010303F^*01010107G^*01010202, A^*0104NB^*070502Cw^*0106E^*010304F^*01010108G^*010103,

A^*0106B^*070503Cw^*0107E^*0104F^*01010201 G^*010104, A^*0107B^*0706Cw^*0108F^*01010202G^*010105, A^*0108B^*0707Cw^*0109F^*01010203G^*010106, A^*0109B^*0708Cw^*01 10F^*01010204G^*010107, A^*01 10B^*0709Cw^*01 1 1 F^*01010205G^*010108,

A^*01 1 1 NB^*0710Cw^*01 12F^*01010301 G^*010109, A^*01 12B^*071 1 Cw^*01 13F^*01010302G^*0101 10, A^*01 13B^*0712Cw^*020201 F^*01010303G^*0102, A^*01 14B^*0713Cw^*020202F^*01010304G^*0103, A^*01 15NB^*0714Cw^*020203F^*0102G^*010401 ,

A^*01 16NB^*0715Cw^*020205F^*01030101 G^*010402,

A^*01 17B^*0716Cw^*0203F^*01030102G^*010403,

A^*01 18NB^*0717Cw^*0204F^*0104G^*0105N, A^*01 19B^*0718Cw^*0205G^*0106,

A^*0120B^*0719Cw^*0206G^*0107, A^*02010101 B^*0720Cw^*0207, A^*02010102LB^*0721 Cw^*0208, A^*020102B^*0722Cw^*0209,

A^*020103B^*0723Cw^*0210, A^*020104B^*0724Cw^*021 1 , A^*020105B^*0725Cw^*0212, A^*020106B^*0726Cw^*0213, A^*020107B^*0727Cw^*0214, A^*020108B^*0728Cw^*0215, A^*020109B^*0729Cw^*0216, A^*0201 10B^*0730Cw^*0217, A^*0201 1 1 B^*0731 Cw^*030201 , A^*0201 12B^*0732Cw^*030202, A^*0202B^*0733Cw^*030301 , A^*020301 B^*0734Cw^*030302, A^*020302B^*0735Cw^*030303,

A^*0204B^*0736Cw^*030304, A^*0205B^*0737Cw^*030305, A^*020601 B^*0738Cw^*030401 , A^*020602B^*0739Cw^*030402, A^*020603B^*0740Cw^*030403, A^*0207B^*0741 Cw^*030404, A^*0208B^*0742Cw^*030405, A^*0209B^*0743Cw^*0305, A^*0210B^*0744Cw^*0306, A^*021 1 B^*0745Cw^*0307, A^*0212B^*0746Cw^*0308, A^*0213B^*0747Cw^*0309, A^*0214B^*0748Cw^*0310, A^*0215NB^*0749NCw^*031 101 ,

A^*0216B^*0750Cw^*031 102, A^*021701 B^*0751 Cw^*0312, A^*021702B^*080101 Cw^*0313, A^*0218B^*080102Cw^*0314, A^*0219B^*080103Cw^*0315, A^*022001 B^*0802Cw^*0316, A^*022002B^*0803Cw^*0317, A^*0221 B^*0804Cw^*0318, A^*0222B^*0805Cw^*0319, A^*0224B^*0806Cw^*0320N, A^*0225B^*0807Cw^*0321 , A^*0226B^*0808NCw^*0322Q, A^*0227B^*0809Cw^*0323, A^*0228B^*0810Cw^*0324, A^*0229B^*081 1 Cw^*0325, A^*0230B^*0812Cw^*0326, A^*0231 B^*0813Cw^*0327, A^*0232NB^*0814Cw^*0328, A^*0233B^*0815Cw^*0329, A^*0234B^*0816Cw^*0330, A^*023501 B^*0817Cw^*0331 , A^*023502B^*0818Cw^*0332, A^*0236B^*0819NCw^*0333, A^*0237B^*0820Cw^*0334, A^*0238B^*0821 Cw^*0335, A^*0239B^*0822Cw^*04010101 , A^*0240B^*0823Cw^*04010102, A^*0241 B^*0824Cw^*040102, A^*0242B^*0825Cw^*040103, A^*0243NB^*0826Cw^*040104,

A^*0244B^*0827Cw^*0403, A^*0245B^*0828Cw^*040401 , A^*0246B^*0829Cw^*040402, A^*0247B^*0830NCw^*0405, A^*0248B^*0831 Cw^*0406, A^*0249B^*1301 Cw^*0407, A^*0250B^*130201 Cw^*0408, A^*0251 B^*130202Cw^*0409N, A^*0252B^*130203Cw^*0410, A^*0253NB^*1303Cw^*041 1 , A^*0254B^*1304Cw^*0412, A^*0255B^*1306Cw^*0413, A^*0256B^*1307NCw^*0414, A^*0257B^*1308Cw^*0415, A^*0258B^*1309Cw^*0416,

A^*0259B^*1310Cw^*0417, A^*0260B^*131 1 Cw^*0418, A^*0261 B^*1312Cw^*0419, A^*0262B^*1313Cw^*0420, A^*0263B^*1314Cw^*0421 , A^*0264B^*1315Cw^*0423, A^*0265B^*1316Cw^*0424, A^*0266B^*1317Cw^*050101 , A^*0267B^*1401 Cw^*050102, A^*0268B^*140201 Cw^*050103, A^*0269B^*140202Cw^*0502, A^*0270B^*1403Cw^*0503, A^*0271 B^*1404Cw^*0504, A^*0272B^*1405Cw^*0505, A^*0273B^*140601 Cw^*0506,

A^*027401 B^*140602Cw^*0507N, A^*027402B^*1407NCw^*0508, A^*0275B^*15010101 Cw^*0509, A^*0276B^*15010102NCw^*0510, A^*0277B^*150102Cw^*051 1 , A^*0278B^*150103Cw^*0512, A^*0279B^*150104Cw^*0513, A^*0280B^*1502Cw^*0514, A^*0281 B^*1503Cw^*0515, A^*0282NB^*1504Cw^*06020101 , A^*0283NB^*1505Cw^*06020102, A^*0284B^*1506Cw^*060202, A^*0285B^*1507Cw^*0603,

A^*0286B^*1508Cw^*0604, A^*0287B^*1509Cw^*0605, A^*0288NB^*1510Cw^*0606, A^*0289B^*151 101 Cw^*0607, A^*0290B^*151 102Cw^*0608, A^*0291 B^*151 103Cw^*0609, A^*0292B^*1512Cw^*0610, A^*0293B^*1513Cw^*061 1 , A^*0294NB^*1514Cw^*0612, A^*0295B^*1515Cw^*0613, A^*0296B^*1516Cw^*0614, A^*0297B^*15170101 Cw^*070101 , A^*0299B^*15170102Cw^*070102, A^*03010101 B^*151702Cw^*070103,

A^*03010102NB^*1518Cw^*070104, A^*03010103B^*1519Cw^*070105, A^*030102B^*1520Cw^*070106, A^*030103B^*1521 Cw^*070107, A^*030104B^*1523Cw^*07020101 , A^*030105B^*1524Cw^*07020102, A^*0302B^*1525Cw^*07020103, A^*0303NB^*1526NCw^*0703, A^*0304B^*1527Cw^*070401 , A^*0305B^*1528Cw^*070402, A^*0306B^*1529Cw^*0705,

A^*0307B^*1530Cw^*0706, A^*0308B^*1531 Cw^*0707, A^*0309B^*1532Cw^*0708, A^*0310B^*1533Cw^*0709, A^*031 1 NB^*1534Cw^*0710, A^*0312B^*1535Cw^*071 1 , A^*0313B^*1536Cw^*0712, A^*0314B^*1537Cw^*0713, A^*0315B^*1538Cw^*0714, A^*0316B^*1539Cw^*0715, A^*0317B^*1540Cw^*0716, A^*0318B^*1542Cw^*0717, A^*0319B^*1543Cw^*0718, A^*0320B^*1544Cw^*0719, A^*0321 NB^*1545Cw^*0720, A^*0322B^*1546Cw^*0721 , A^*0323B^*1547Cw^*0722, A^*0324B^*1548Cw^*0723, A^*0325B^*1549Cw^*0724, A^*0326B^*1550Cw^*0725, A^*1 10101 B^*1551 Cw^*0726, A^*1 10102B^*1552Cw^*0727, A^*1 10103B^*1553Cw^*0728, A^*1 10104B^*1554Cw^*0729, A^*1 10105B^*1555Cw^*0730, A^*1 10106B^*1556Cw^*0731 , A^*1 10201 B^*1557Cw^*0732N, A^*1 10202B^*1558Cw^*0733N, A^*1 103B^*1560Cw^*0734, A^*1 104B^*1561 Cw^*0735,

A^*1 105B^*1562Cw^*0736, A^*1 106B^*1563Cw^*0737, A^*1 107B^*1564Cw^*0738, A^*1 108B^*1565Cw^*0739, A^*1 109B^*1566Cw^*0740, A^*1 1 10B^*1567Cw^*0741 , A^*1 1 1 1 B^*1568Cw^*0742, A^*1 1 12B^*1569Cw^*0743, A^*1 1 13B^*1570Cw^*0744, A^*1 1 14B^*1571 Cw^*0745, A^*1 1 15B^*1572Cw^*080101 , A^*1 1 16B^*1573Cw^*080102, A^*1 1 17B^*1574Cw^*0802, A^*1 1 18B^*1575Cw^*0803, A^*1301 B^*1576Cw^*0804,

A^*1 120B^*1577Cw^*0805, A^*1 121 NB^*1578Cw^*0806, A^*1 122B^*1579NCw^*0807, A^*1 123B^*1580Cw^*0808, A^*1 124B^*1581 Cw^*0809, A^*1 125B^*1582Cw^*0810, A^*1 126B^*1583Cw^*081 1 , A^*1 127B^*1584Cw^*0812, A^*1 128B^*1585Cw^*0813, A^*1 129B^*1586Cw^*0814, A^*2301 B^*1587Cw^*120201 , A^*2302B^*1588Cw^*120202, A^*2303B^*1589Cw^*120203, A^*2304B^*1590Cw^*12030101 ,

A^*2305B^*1591 Cw^*12030102, A^*2306B^*1592Cw^*120302, A^*2307NB^*1593Cw^*120303, A^*2308NB^*1594NCw^*120304, A^*2309B^*1595Cw^*120401 , A^*2310B^*1596Cw^*120402, A^*231 1 NB^*1597Cw^*1205, A^*2312B^*1598Cw^*1206, A^*2313B^*1599Cw^*1207, A^*2314B^*9501 Cw^*1208, A^*24020101 B^*9502Cw^*1209, A^*24020102LB^*9503Cw^*1210,

A^*240202B^*9504Cw^*121 1 , A^*240203B^*9505Cw^*1212, A^*240204B^*9506Cw^*1213, A^*240205B^*9507Cw^*1214, A^*240206B^*9508Cw^*1215, A^*240207B^*9509Cw^*1216, A^*240208B^*9510Cw^*1217, A^*240209B^*951 1 NCw^*1218, A^*240210B^*9512Cw^*1219, A^*24021 1 B^*9513Cw^*140201 , A^*240212B^*9514Cw^*140202, A^*240213B^*9515Cw^*140203, A^*240301 B^*9516Cw^*140204,

A^*240302B^*9517Cw^*1403, A^*2404B^*9518Cw^*1404, A^*2405B^*9519Cw^*1405, A^*2406B^*9520Cw^*1406, A^*2407B^*9521 Cw^*1407N, A^*2408B^*9522Cw^*1408, A^*2409NB^*180101 Cw^*150201 , A^*2410B^*180102Cw^*150202, A^*241 1 NB^*180103Cw^*150203, A^*2413B^*1802Cw^*1503, A^*2414B^*1803Cw^*1504, A^*2415B^*1804Cw^*150501 , A^*2417B^*1805Cw^*150502, A^*2418B^*1806Cw^*150503,

A^*2419B^*1807Cw^*150504, A^*2420B^*1808Cw^*1506, A^*2421 B^*1809Cw^*1507, A^*2422B^*1810Cw^*1508, A^*2423B^*181 1 Cw^*1509, A^*2424B^*1812Cw^*1510, A^*2425B^*1813Cw^*151 1 , A^*2426B^*1814Cw^*1512, A^*2427B^*1815Cw^*1513, A^*2428B^*1817NCw^*1514, A^*2429B^*1818Cw^*1515, A^*2430B^*1819Cw^*1516, A^*2431 B^*1820Cw^*1517, A^*2432B^*1821 Cw^*160101 , A^*2433B^*1822Cw^*160102, A^*2434B^*1823NCw^*1602, A^*2435B^*1824Cw^*160401 , A^*2436NB^*2701 Cw^*1606, A^*2437B^*2702Cw^*1607, A^*2438B^*2703Cw^*1608, A^*2439B^*270401 Cw^*1609, A^*2440NB^*270402Cw^*1701 , A^*2441 B^*270502Cw^*1702, A^*2442B^*270503Cw^*1703, A^*2443B^*270504Cw^*1704, A^*2444B^*270505Cw^*1801 , A^*2445NB^*270506Cw^*1802, A^*2446B^*270507, A^*2447B^*270508, A^*2448NB^*270509, A^*2449B^*2706,

A^*2450B^*2707, A^*2451 B^*2708, A^*2452B^*2709, A^*2453B^*2710, A^*2454B^*271 1 , A^*2455B^*2712, A^*2456B^*2713, A^*2457B^*2714, A^*2458B^*2715, A^*2459B^*2716, A^*2460NB^*2717, A^*2461 B^*2718, A^*2462B^*2719, A^*2463B^*2720, A^*2464B^*2721 , A^*2465B^*2723, A^*2466B^*2724, A^*2467B^*2725, A^*2468B^*2726, A^*250101 B^*2727, A^*250102B^*2728, A^*2502B^*2729, A^*2503B^*2730, A^*2504B^*2731 , A^*2505B^*2732,

A^*2506B^*2733, A^*260101 B^*2734, A^*260102B^*2735, A^*260103B^*2736, A^*260104B^*350101 , A^*2602B^*350102, A^*2603B^*350103, A^*2604B^*350104, A^*2605B^*350105, A^*2606B^*350106, A^*260701 B^*350201 , A^*260702B^*350202, A^*2608B^*3503, A^*2609B^*350401 , A^*2610B^*350402, A^*261 1 NB^*3505, A^*2612B^*3506, A^*2613B^*3507, A^*2614B^*350801 , A^*2615B^*350802,

A^*2616B^*350901 , A^*2617B^*350902, A^*2618B^*3510, A^*2619B^*351 1 , A^*2620B^*3512, A^*2621 B^*3513, A^*2622B^*351401 , A^*2623B^*351402, A^*2624B^*3515, A^*2625NB^*3516, A^*2626B^*3517, A^*2627B^*3518, A^*2628B^*3519, A^*2629B^*3520, A^*2630B^*3521 , A^*2631 B^*3522, A^*2632B^*3523, A^*2633B^*3524, A^*2634B^*3525, A^*29010101 B^*3526, A^*29010102NB^*3527, A^*290201 B^*3528, A^*290202B^*3529,

A^*290203B^*3530, A^*2903B^*3531 , A^*2904B^*3532, A^*2905B^*3533, A^*2906B^*3534, A^*2907B^*3535, A^*2908NB^*3536, A^*2909B^*3537, A^*2910B^*3538, A^*291 1 B^*3539, A^*2912B^*3540N, A^*2913B^*3541 , A^*2914B^*3542, A^*2915B^*3543, A^*2916B^*3544, A^*300101 B^*3545, A^*300102B^*3546, A^*300201 B^*3547, A^*300202B^*3548, A^*300203B^*3549, A^*3003B^*3550, A^*3004B^*3551 , A^*3006B^*3552, A^*3007B^*3553N,

A^*3008B^*3554, A^*3009B^*3555, A^*3010B^*3556, A^*301 1 B^*3557, A^*3012B^*3558, A^*3013B^*3559, A^*3014LB^*3560, A^*3015B^*3561 , A^*3016B^*3562, A^*3017B^*3563, A^*3018B^*3564, A^*3019B^*3565Q, A^*310102B^*3566, A^*3102B^*3567, A^*3103B^*3568, A^*3104B^*3569, A^*3105B^*3570, A^*3106B^*3571 , A^*3107B^*3572, A^*3108B^*370101 , A^*3109B^*370102, A^*31 10B^*370103, A^*31 1 1 B^*370104, A^*31 12B^*3702,

A^*31 13B^*3703N, A^*31 14NB^*3704, A^*31 15B^*3705, A^*3201 B^*3706, A^*3202B^*3707, A^*3203B^*3708, A^*3204B^*3709, A^*3205B^*3710, A^*3206B^*371 1 , A^*3207B^*3712, A^*3208B^*380101 , A^*3209B^*380102, A^*3210B^*380201 , A^*321 1 QB^*380202, A^*3212B^*3803, A^*3213B^*3804, A^*3214B^*3805, A^*3301 B^*3806, A^*330301 B^*3807, A^*330302B^*3808, A^*3304B^*3809, A^*3305B^*3810, A^*3306B^*381 1 , A^*3307B^*3812, vJT"

A^*3308B^*3813, A^*3309B^*3814, A^*3401 B^*3815, A^*3402B^*39010101 , A^*3403B^*39010102L, A^*3404B^*390103, A^*3405B^*390104, A^*3406B^*390201 , A^*3407B^*390202, A^*3408B^*3903, A^*3601 B^*3904, A^*3602B^*3905, A^*3603B^*390601 , A^*3604B^*390602, A^*4301 B^*3907, A^*6601 B^*3908, A^*6602B^*3909, A^*6603B^*3910, A^*6604B^*391 1 , A^*6605B^*3912, A^*6606B^*391301 , A^*680101 B^*391302,

A^*680102B^*3914, A^*680103B^*3915, A^*680104B^*3916, A^*680105B^*3917, A^*68020101 B^*3918, A^*68020102B^*3919, A^*680301 B^*3920, A^*680302B^*3922, A^*6804B^*3923, A^*6805B^*3924, A^*6806B^*3925N, A^*6807B^*3926, A^*6808B^*3927, A^*6809B^*3928, A^*6810B^*3929, A^*681 1 NB^*3930, A^*6812B^*3931 , A^*6813B^*3932, A^*6814B^*3933, A^*6815B^*3934, A^*6816B^*3935, A^*6817B^*3936, A^*6818NB^*3937,

A^*6819B^*3938Q, A^*6820B^*3939, A^*6821 B^*3940N, A^*6822B^*3941 , A^*6823B^*400101 , A^*6824B^*400102, A^*6825B^*400103, A^*6826B^*400104, A^*6827B^*400105, A^*6828B^*400201 , A^*6829B^*400202, A^*6830B^*400203, A^*6831 B^*4003, A^*6832B^*4004, A^*6833B^*4005, A^*6834B^*40060101 , A^*6835B^*40060102, A^*6836B^*400602, A^*6901 B^*4007, A^*7401 B^*4008,

A^*7402B^*4009, A^*7403B^*4010, A^*7404B^*401 1 , A^*7405B^*4012, A^*7406B^*4013, A^*7407B^*401401 , A^*7408B^*401402, A^*7409B^*401403, A^*7410B^*4015, A^*741 1 B^*4016, A^*7412NB^*4018, A^*8001 B^*4019, A^*9201 B^*4020, A^*9202B^*4021 , A^*9203B^*4022N, A^*9204B^*4023, A^*9205B^*4024, A^*9206B^*4025, A^*9207B^*4026, A^*9208B^*4027, A^*9209B^*4028, B^*4029, B^*4030, B^*4031 , B^*4032, B^*4033, B^*4034,

B^*4035, B^*4036, B^*4037, B^*4038, B^*4039, B^*4040, B^*4042, B^*4043, B^*4044, B^*4045, B^*4046, B^*4047, B^*4048, B^*4049, B^*4050, B^*4051 , B^*4052, B^*4053, B^*4054, B^*4055, B^*4056, B^*4057, B^*4058, B^*4059, B^*4060, B^*4061 , B^*4062, B^*4063, B^*4064, B^*4065, B^*4066, B^*4067, B^*4068, B^*4069, B^*4070, B^*4101 , B^*4102, B^*4103, B^*4104, B^*4105, B^*4106, B^*4107, B^*4108, B^*4201 , B^*4202, B^*4204, B^*420501, B^*420502, B^*4206, B^*4207, B^*4208, B^*4209, B^*44020101, B^*44020102S, B^*440202, B^*440203, B^*440204, B^*440301, B^*440302, B^*4404, B^*4405, B^*4406, B^*4407, B^*4408, B^*4409, B^*4410, B^*4411, B^*4412, B^*4413, B^*4414, B^*4415, B^*4416, B^*4417, B^*4418, B^*4419N, B^*4420, B^*4421, B^*4422, B^*4423N, B^*4424, B^*4425, B^*4426, B^*4427, B^*4428, B^*4429, B^*4430, B^*4431 , B^*4432, B^*4433, B^*4434, B^*4435, B^*4436, B^*4437, B^*4438, B^*4439, B^*4440, B^*4441, B^*4442, B^*4443, B^*4444, B^*4445, B^*4446, B^*4447, B^*4448, B^*4449, B^*4450, B^*4451, B^*4501, B^*4502, B^*4503, B^*4504, B^*4505, B^*4506, B^*4507, B^*460101, B^*460102, B^*4602, B^*4603, B^*4604, B^*4605, B^*4606, B^*4607N, B^*4608, B^*4609, B^*47010101 , B^*47010102, B^*4702, B^*4703, B^*4704, B^*4705, B^*4801 , B^*4802, B⁴480301 , B⁴480302, B⁴4804, B⁴4805, B^*4806, B⁴4807, B⁴4808, B⁴4809, B⁴4810, B^*481 1 , B⁴4812, B⁴4813, B⁴4814, B⁴4815, B^*4816, B⁴4901 , B⁴4902, B⁴4903, B⁴4904, B^*4905, B⁴5001 , B⁴5002, B⁴5004, B⁴510101 , B^*510102, B⁴510103, B⁴510104, B⁴510105, B⁴510106, B^*510107, B⁴510201 , B⁴510202, B⁴5103, B⁴5104, B^*5105, B⁴5106, B^*5107, B⁴5108, B⁴5109, B^*51 10, B⁴51 1 1 N, B^*51 12, B^*51 1301 ,

B^*51 1302, B⁴51 14, B⁴51 15, B⁴51 16, B^*51 17, B^*51 18, B^*51 19, B^*5120, B^*5121 , B^*5122, B^*5123, B^*5124, B^*5126, B^*5127N, B^*5128, B^*5129, B^*5130, B^*5131 , B^*5132, B^*5133, B^*5134, B^*5135, B^*5136, B^*5137, B^*5138, B^*5139, B^*5140, B^*5141 N, B^*5142, B^*5143, B^*5144N, B^*5145, B^*5146, B^*520101 , B^*520102, B^*520103, B^*520104, B^*5202, B^*5203, B^*5204, B^*5205, B^*5206, B^*5207, B^*5208,

B^*5209, B^*5210, B^*530101 , B^*530102, B^*530103, B^*530104, B^*5302, B^*5303, B^*5304, B^*5305, B^*5306, B^*5307, B^*5308, B^*5309, B^*5310, B^*531 1 , B^*5312, B^*5401 , B^*5402, B^*5403, B^*5404, B^*5405N, B^*5406, B^*5407, B^*5408N, B^*5409, B^*5410, B^*541 1 , B^*5412, B^*550101 , B^*550102, B^*550103, B^*550104, B^*550201 , B^*550202, B^*5503, B^*5504, B^*5505, B^*5507, B^*5508, B^*5509, B^*5510, B^*5511 , B^*5512, B^*5513, B^*5514, B^*5515, B^*5516, B^*5517, B^*5518, B^*5519, B^*5520, B^*5521, B^*5522, B^*5523, B^*5524, B^*5601 , B^*5602, B^*5603, B^*5604, B^*560501, B^*560502, B^*5606, B^*5607, B^*5608, B^*5609, B^*5610, B^*5611, B^*5612, B^*5613, B^*5614, B^*5615, B^*5616, B^*5617, B^*5618, B^*5619N, B^*570101, B^*570102, B^*570103, B^*5702, B^*570301 , B^*570302, B^*5704, B^*5705, B^*5706, B^*5707, B^*5708, B^*5709, B^*5710, B^*5711, B^*5801, B^*5802, B^*5804, B^*5805, B^*5806, B^*5807, B^*5808, B^*5809, B^*5810N, B^*5811, B^*5812, B^*5813, B^*5814, B^*5901, B^*5902, B^*670101, B^*670102, B^*6702, B^*7301, B^*7801, B^*780201, B^*780202, B^*7803, B^*7804, B^*7805, B^*8101, B^*8102, B^*8201, B^*8202, B^*8301, H⁴OI OI OI OI J⁴OI OI OI OI K⁴OI OI OI OI L⁴OI OI OI OI P⁴OI OI OI OI ,

H⁴01010102J⁴01010102K⁴01010102L⁴01010102P⁴01010102, H^*01010103J^*01010103K^*01010103L^*01010103P^*02010101 , H⁴OI 02J⁴01010104K⁴01010104L⁴010102P⁴02010102, H⁴02010101 J⁴OI 010105K⁴0102L⁴0102, H⁴02010102J⁴01010106K⁴0103, H⁴0202J⁴01010107, H⁴0203J⁴01010108, H⁴0204J⁴0201 , H⁴0205, H⁴0206 and,

H⁴0301 .

1 18. The MHC multimer according to any of claims 1 to 1 16, wherein the MHC protein is the same or different MHC proteins selected from the group of HLA classes consisting of A⁴0201 , C⁴0701 , A⁴0101 , A⁴0301 , C⁴0702, C⁴0401 , B⁴4402, B⁴0702, B^*0801 , C^*0501 , C^*0304, C^*0602, A^*1 101 , B^*4001 , A^*2402, B^*3501 , C^*0303, B^*5101 , C^*1203, B^*1501 , A^*2902, A^*2601 , A^*3201 , C^*0802, A^*2501 , B^*5701 , B^*1402, C^*0202, B^*1801 , B^*4403, C^*0401 , C^*0701 , C^*0602, A^*0201 , A^*2301 , C^*0202, A^*0301 , C^*0702, B^*5301 , B^*0702, C^*1601 , B^*1503, B^*5801 , A^*6802, C^*1701 , B^*4501 , B^*4201 , A^*3001 , B^*3501 , A^*0101 , C^*0304, A^*3002, B^*0801 ,

A^*3402, A^*7401 , A^*3303, C^*1801 , A^*2902, B^*4403, B^*4901 , A^*0201 , C^*0401 , A^*2402, C^*0702, C^*0701 , C^*0304, A^*0301 , B^*0702, B^*3501 , C^*0602, C^*0501 , A^*0101 , A^*1 101 , B^*5101 , C^*1601 , B^*4403, C^*0102, A^*2902, C^*0802, B^*1801 , A^*3101 , B^*5201 , B^*1402, C^*0202, C^*1203, A^*2601 , A^*6801 , B^*0801 , A^*3002, B^*4402, A^*1 101 , A^*2402, C^*0702, C^*0102, A^*3303, C^*0801 , C^*0304, A^*0201 ,

B^*4001 , C^*0401 , B^*5801 , B^*4601 , B^*5101 , C^*0302, B^*3802, A^*0207, B^*1501 , A^*0206, C^*0303, B^*1502, A^*0203, B^*4403, C^*1402, B^*3501 , C^*0602, B^*5401 , B^*1301 , B^*4002, B^*5502, A^*2601.

1 19. The MHC multimer according to any claims 1 to 1 16 comprising one or more covalently attached labels.

120. The MHC multimer according to any of claims 1 to 1 16 comprising one or more non-covalently attached labels.

121. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to the polypeptide a.

122. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to the polypeptide b.

123. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to the peptide P.

124. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to the one or more multimerization domains.

125. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to (a-b-P)_n. *

126. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to the polypeptide a.

127. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to the polypeptide b.

128. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to P.

129. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to the one or more multimerization domains.

130. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to (a-b-P)_n.

131 . The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to the antibody in the mutimerization domain.

132. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to an antibody in the mutimerization domain.

133. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to an aptamer in the mutimerization domain.

134. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to an aptamer in the mutimerization domain.

135. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to a molecule in the mutimerization domain.

136. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to a molecule in the mutimerization domain.

137. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to a protein in the mutimerization domain.

138. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to a protein in the mutimerization domain.

139. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to a sugar residue in the mutimerization domain.

140. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to a sugar residue in the mutimerization domain.

141. The MHC multimer according to claim 120, wherein the one or more labels is non-covalently attached to a DNA in the mutimerization domain.

142. The MHC multimer according to claim 1 19, wherein the one or more labels is covalently attached to a DNA in the mutimerization domain.

143. The MHC multimer according to any of the claims 1 19 to 142, wherein the attachment is directly between reactive groups in the labelling molecule and reactive groups in the marker molecule.

144. The MHC multimer according to any of the claims 1 19 to 142, wherein the attachment is through a linker connecting labelling molecule and marker.

145. The MHC multimer according to any of the claims 1 19 to 144, wherein one label is used.

146. The MHC multimer according to any of the claims 1 19 to 144, wherein more than one label is used.

147. The MHC multimer according to claim 146, wherein the more than one label are all identical.

148. The MHC multimer according to claim 146, wherein at least two labels are different.

149. The MHC multimer according to any of claims 1 19 to 148, wherein the one or more labels is attached to (a-b-P)_n via a streptavidin-biotin linkage.

150. The MHC multimer according to any of claims 1 19 to 148, wherein the one or more labels is a fluorophore label.

151 . The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group of fluorescein isothiocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde and fluorescamine.

152. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 2-(4'- maleimidylanilino)naphthalene-6- sulfonic acid, sodium salt

153. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 5-((((2- iodoacetyl)amino)ethyl)amino) naphthalene-1 -sulfonic acid

154. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Pyrene-1 -butanoic acid

155. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of AlexaFluor 350 (7-amino- 6-sulfonic acid-4-methyl coumarin-3-acetic acid

156. The MHC multimer according to claim 150 wherein the one or more fluorophore label are selected from the group consisting of AMCA (7-amino-4-methyl coumarin-3-acetic acid

157. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 7-hydroxy-4-methyl coumarin-3-acetic acid

158. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Marina Blue (6,8-difluoro- 7-hydroxy-4-methyl coumarin-3-acetic acid

159. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 7-dimethylamino- coumarin-4-acetic acid

160. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Fluorescamin-N-butyl amine adduct

161 . The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 7-hydroxy-coumarine-3- carboxylic acid

162. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of CascadeBIue (pyrene- trisulphonic acid acetyl azide

163. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Cascade Yellow

164. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Pacific Blue (6,8 difluoro-

7-hydroxy coumarin-3-carboxylic acid

165. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 7-diethylamino-coumarin- 3-carboxylic acid

166. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of N-(((4- azidobenzoyl)amino)ethyl)- 4-amino-3,6-disulfo-1 ,8-naphthalimide, dipotassium salt

167. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Alexa Fluor 430

168. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 3-perylenedodecanoic acid

169. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 8-hydroxypyrene-1 ,3,6- trisulfonic acid, trisodium salt

170. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 12-(N-(7-nitrobenz-2-oxa- 1 ,3- diazol-4-yl)amino)dodecanoic acid

171 . The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of N,N'-dimethyl-N- (iodoacetyl)-N'-(7-nitrobenz-2- oxa-1 ,3-diazol-4-yl)ethylenediamine

172. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Oregon Green 488 (difluoro carboxy fluorescein)

173. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of 5- iodoacetamidofluorescein

174. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of propidium iodide-DNA add uct

175. The MHC multimer according to claim 150, wherein the one or more fluorophore label are selected from the group consisting of Carboxy fluorescein

176. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a fluorescent label.

177. The MHC multimer according to claim 176, wherein the one or more fluorescent label is a simple fluorescent label

178. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group Fluor dyes, Pacific Blue™, Pacific Orange™, Cascade Yellow™

179. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group AlexaFluor@(AF), AF405, AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750, AF800.

180. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group Quantum Dot based dyes, QDot® Nanocrystals (Invitrogen, MolecularProbs), Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800.

181. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group DyLight™ Dyes (Pierce) (DL); DL549, DL649, DL680, DL800.

182. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group Fluorescein (Flu) or any derivate of that, such as FITC

183. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group Cy-Dyes, Cy2, Cy3, Cy3.5, Cy5,

Cy5.5, Cy7.

184. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group Fluorescent Proteins, RPE, PerCp, APC, Green fluorescent proteins; GFP and GFP derived mutant proteins; o

BFP, CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine, mStrawberry, mCherry.

185. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group Tandem dyes, RPE-Cy5, RPE-

Cy5.5, RPE-Cy7, RPE-AlexaFluor® tandem conjugates; RPE-Alexa610, RPE-TxRed, APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5, APC-Cy5.5.

186. The MHC multimer according to claim 177, wherein the one or more simple fluorescent label is selected from the group multi fluorochrome assemblies,

Multiple fluorochromes attached to a polymer molecule, such as a peptide/protein, Dextrane, polysaccharide, any combination of the fluorescent dyes involving in generation of FRET (Fluorescence resonance energy transfer) based techniques.

187. The MHC multimer according to claim 177 wherein the one or more simple fluorescent label is selected from the group ionophors; ion chelating fluorescent props, props that change wavelength when binding a specific ion, such as Calcium, props that change intensity when binding to a specific ion, such as Calcium.

188. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is capable of absorption of light

189. The MHC multimer according to claim 188, wherein the one or more labels capable of absorption of light is a chromophore.

190. The MHC multimer according to claim 188, wherein the one or more labels capable of absorption of light is a dye.

191. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is capable of emission of light after excitation

192. The MHC multimer according to claim 191 , wherein the one or more labels capable of emission of light is one or more fluorochromes.

193. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the AlexaFluor@(AF) family, which include AF®350, AF405, AF430, AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750 and AF800

194. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the Quantum Dot (Qdol®) based dye family, which include Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800

195. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the DyLight™ Dyes (DL) family, which include DL549, DL649, DL680, DL800

196. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Small fluorescing dyes, which include FITC, Pacific Blue™, Pacific Orange™, Cascade Yellow™, Marina blue™, DSred, DSred-2, 7-AAD, TO-Pro-3.

197. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Cy-Dyes, which include Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7

198. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Phycobili Proteins, which include R-

Phycoerythrin (RPE), PerCP, Allophycocyanin (APC), B-Phycoerythrin, C- Phycocyanin.

199. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Fluorescent Proteins, which include

(E)GFP and GFP ((enhanced) green fluorescent protein) derived mutant proteins; BFP, CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine.

200. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Tandem dyes with RPE, which include RPE-Cy5, RPE-Cy5.5, RPE-Cy7, RPE-AlexaFIuor® tandem conjugates; RPE- Alexa610, RPE-TxRed.

201. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Tandem dyes with APC, which include APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5, APC-Cy5.5.

202. The MHC multimer according to claim 192, wherein the one or more fluorochrome is selected from the family of Calcium dyes, which include Indo-1 -Ca²⁺ lndo-2-Ca²⁺.

203. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is capable of reflection of light.

204. The MHC multimer according to claim 203, wherein the one or more labels capable of reflection of light comprises gold.

205. The MHC multimer according to claim 203, wherein the one or more labels capable of reflection of light comprises plastic.

206. The MHC multimer according to claim 203, wherein the one or more labels capable of reflection of light comprises glass.

207. The MHC multimer according to claim 203, wherein the one or more labels capable of reflection of light comprises polystyrene.

208. The MHC multimer according to claim 203, wherein the one or more labels capable of reflection of light comprises pollen.

209. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a chemiluminescent label.

210. The MHC multimer according to claim 209, wherein the chemiluminescent labels is selected from the group luminol, isoluminol, theromatic acridinium ester, imidazole, acridinium salt and oxalate ester.

21 1. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a bioluminescent label.

212. The MHC multimer according to claim 21 1 , wherein the bioluminescent labels is selected from the group luciferin, luciferase and aequorin.

213. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a radioactive label.

214. The MHC multimer according to claim 213, wherein the one or more radioactive labels is a radionuclide.

215. The MHC multimer according to claim 213 wherein the one or more radioactive labels is an isotope.

216. The MHC multimer according to claim 213, wherein the one or more radioactive labels comprises α rays.

217. The MHC multimer according to claim 213, wherein the one or more radioactive labels comprises β rays.

218. The MHC multimer according to claim 213, wherein the one or more radioactive labels comprises y rays.

219. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is detectable by NMR (nuclear magnetic resonance form paramagnetic molecules).

220. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is an enzyme label.

221. The MHC multimer according to claim 220, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, producing a light signal (chemi-luminescence).

222. The MHC multimer according to claim 220, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, resulting in precipitation of chromophor dyes.

223. The MHC multimer according to claim 220, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, resulting in precipitates that can be detected by an additional layer of detection molecules.

224. The MHC multimer according to claim 220, wherein the enzyme label is selected from the group peroxidases, malate dehydrogenase, staphylococcal nuclease, delta-5-steroid isomerase, yeast alcohol dehydrogenase, alpha- glycerophosphate, dehydrogenase, triose phosphate isomerase, horseradish peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta- galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase and acetylcholinesterase.

225. The MHC multimer according to claim 220, wherein the enzyme label is horseradish peroxidase.

226. The MHC multimer according to claim 220, wherein the enzyme label is horseradish peroxidase and the substrate is diaminobenzidine (DAB).

227. The MHC multimer according to claim 220, wherein the enzyme label is horseradish peroxidase and the substrate is 3-amino-9-ethyl-carbazole (AEC+).

228. The MHC multimer according to claim 220, wherein the enzyme label is horseradish peroxidase and the substrate is biotinyl tyramide.

229. The MHC multimer according to claim 220, wherein the enzyme label is horseradish peroxidase and the substrate is fluorescein tyramide.

230. The MHC multimer according to claim 220, wherein the enzyme label is alkaline phosphatase.

231. The MHC multimer according to claim 220, wherein the enzyme label is alkaline phosphatase and the substrate is Fast red dye.

232. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a ionophore or chelating chemical compound binding to specific ions such as Ca²⁺.

233. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a lanthanide.

234. The MHC multimer according to claim 233, wherein the lanthanide comprises fluorescence.

235. The MHC multimer according to claim 233, wherein the lanthanide comprises Phosphorescence.

236. The MHC multimer according to claim 233, wherein the lanthanide is paramagnetic.

237. The MHC multimer according to any of claims 1 19-148, wherein the one or more labels is a DNA fluorescing stain.

238. The MHC multimer according to claim 237, wherein the DNA fluorescing stain is Propidium iodide.

239. The MHC multimer according to claim 237, wherein the DNA fluorescing stain is Hoechst stain.

240. The MHC multimer according to claim 237, wherein the DNA fluorescing stain is DAPI.

241. The MHC multimer according to claim 237, wherein the DNA fluorescing stain is AMC.

242. The MHC multimer according to claim 237, wherein the DNA fluorescing stain is DraQ5™

243. The MHC multimer according to claim 237 wherein the DNA fluorescing stain is Acridine orange.

244. The MHC multimer according to claim 1 , wherein the MHC-peptide complex (a-b-P) is attached to a multimerization domain comprising an avidin or streptavidin via a linkage comprising a biotin moiety.

245. The MHC multimer according to any of claims 1 to 244, wherein P is chemically modified.

246. The MHC multimer according to any of claims 1 to 244, wherein P is pegylated.

247. The MHC multimer according to any of claims 1 to 244, wherein P is phosphorylated.

248. The MHC multimer according to any of claims 1 to 244, wherein P is glycosylated.

249. The MHC multimer according to any of claims 1 to 244, wherein one of the amino acid residues of the peptide P is substituted with another amino acid.

250. The MHC multimer according to any of claims 1 to 244, wherein a and b are both full-length peptides.

251. The MHC multimer according to any of claims 1 to 244, wherein a is a full-length peptide.

252. The MHC multimer according to any of claims 1 to 244, wherein b is a full-length peptide.

253. The MHC multimer according to any of claims 1 to 244, wherein a is truncated.

254. The MHC multimer according to any of claims 1 to 244, wherein b is truncated.

255. The MHC multimer according to any of claims 1 to 244, wherein a and b are both truncated.

256. The MHC multimer according to any of claims 1 to 244, wherein a is covalently linked to b.

257. The MHC multimer according to any of claims 1 to 244, wherein a is covalently linked to P.

258. The MHC multimer according to any of claims 1 to 244, wherein b is covalently linked to P.

259. The MHC multimer according to any of claims 1 to 244, wherein a, b and P are all covalently linked.

260. The MHC multimer according to any of claims 1 to 244, wherein a is non- covalently linked to b.

261. The MHC multimer according to any of claims 1 to 244, wherein a is non- covalently linked to P.

262. The MHC multimer according to any of claims 1 to 244, wherein b is non- covalently linked to P.

263. The MHC multimer according to any of claims 1 to 244, wherein a, b and P are all non-covalently linked. O

264. The MHC multimer according to any of claims 1 to 244, wherein a is not included in the (a-b-P) complex.

265. The MHC multimer according to any of claims 1 to 244, wherein b is not included in the (a-b-P) complex.

266. The MHC multimer according to any of claims 1 to 244, wherein P is not included in the (a-b-P) complex.

267. The MHC multimer according to any of claims 1 to 244, wherein P consists of 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 , 22, 23, 24, 25, 26, 27, 28, 29, or 30 amino acids.

268. The MHC multimer according to any of claims 1 to 244, wherein P consist of more than 30 amino acids such as/or more than 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95 or 100 amino acids.

269. The MHC multimer according to any of claims 1 to 244, wherein the MHC-peptide complex is linked to at least one of the one or more multimerization domains by a linker moiety.

270. The MHC multimer according to claim 269, wherein the MHC-peptide complex is linked to at least one of the one or more multimerization domains by a covalent linker moiety.

271. The MHC multimer according to claims 269 and 270, wherein the linkage of at least one of the one or more multimerization domains and at least one MHC- peptide complexes is formed by a binding entity X attached to, or being part of, at least one of the one or more multimerization domains, and a binding entity Y attached to, or being part of at least one of the MHC-peptide complexes.

272. The MHC multimer according to claim 269 and 270, wherein the linker moiety linking at least one of the one or more multimerization domains and the MHC- peptide complex comprises the linker moiety XY, wherein the linker moiety XY results from a reaction of the moiety X comprising one or more reactive groups and the moiety Y comprising one or more reactive groups, wherein at least some of said reactive groups are capable of reacting with each other.

273. The MHC multimer according to any of claims 271 and 272, wherein the moiety X comprises a nucleophilic group.

274. The MHC multimer according to claim 273, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -NH-NH₂.

275. The MHC multimer according to claim 272, wherein the moiety Y comprises an electrophilic group.

276. The MHC multimer according to claim 275, wherein the electrophilic group is selected from the group consisting of CHO, COOH and CO.

277. The MHC multimer according to claims 269 and 270, wherein at least one of the reactive groups on one of the moieties X and Y comprises a radical capable of reacting with a reactive group forming part of the other moiety.

278. The MHC multimer according to claims 269 and 270, wherein X and Y comprises reactive groups natively associated with the one or more multimerization domains and/or the MHC-peptide complexes.

279. The MHC multimer according to claims 269 and 270, wherein X and Y comprises reactive groups not natively associated with the one or more multimerization domains and/or the MHC-peptide complex.

280. The MHC multimer according to claims 269 and 270, wherein the linker moiety forms a covalent link between at least one of the one or more multimerization domains and at least one of the MHC-peptide complexes.

281. The MHC multimer according to claims 269 and 270, wherein the reactive groups of MHC-peptide complexes include amino acid side chains selected from the group -NH₂, -OH, -SH, and -NH-

282. The MHC multimer according to claims 277 and 279, wherein the reactive groups of multimerization domains include hydroxyls of polysaccharides such as dextrans.

283. The MHC multimer according to claims 277 and 279, wherein the reactive groups of multimerization domains are selected from the group amino acid side chains comprising -NH₂, -OH, -SH, and -NH- of polypeptides.

284. The MHC multimer according to claims 269 and 270, wherein one of the polypeptides of the MHC-peptide complex is linked by a protein fusion to the multimerization domain.

285. The MHC multimer according to claims 269 and 270, wherein one of the polypeptides of the MHC-peptide complex is linked by a protein fusion to the multimerization domain, wherein an acyl group and an amino group react to form an amide bond.

286. The MHC multimer according to claim 1 , wherein one of the polypeptides of the MHC-peptide complex is a β2M polypeptide.

287. The MHC multimer according to claim 1 , wherein one of the polypeptides of the MHC-peptide complex is a heavy chain polypeptide.

288. The MHC multimer according to claim 1 , wherein one of the polypeptides of the MHC-peptide complex is an antigenic peptide.

289. The MHC multimer according to claim 1 , wherein one of the polypeptides of the MHC-peptide complex is linked by non-native reactive groups to the multimerization domain.

290. The MHC multimer according to claim 289, wherein the non-native reactive groups include reactive groups that are attached to the multimerization domain through association of a linker molecule comprising the reactive group.

291. The MHC multimer according to claim 289, wherein the non-native reactive groups include reactive groups that are attached to the MHC-peptide complex through association of a linker molecule comprising the reactive group.

292. The MHC multimer according to claim 291 , wherein a dextran is activated by reaction of the dextran hydroxyls with divinyl sulfon.

293. The MHC multimer according to claim 291 , wherein dextran is activated by a multistep reaction that results in the decoration of the dextran with maleimide groups.

294. The MHC multimer according to claim 1 , wherein the multimerization domain comprises one or more nucleophilic groups.

295. The MHC multimer according to claim 294, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -CN, -NH-NH₂.

296. The MHC multimer according to claim 1 , wherein the multimerization domain is selected from the group polysaccharides, polypeptides comprising e.g. lysine, serine, and cysteine.

297. The MHC multimer according to claim 1 , wherein the multimerization domain comprises one or more electrophilic groups.

298. The MHC multimer according to claim 297, wherein the electrophilic group is selected from the group -COOH, -CHO, -CO, NHS-ester, tosyl-activated ester, and other activated esters, acid-anhydrides.

299. The MHC multimer according to claim 1 , wherein reactive groups involved in forming an association between the multimerization domain and the MHC peptide complex are located on glutamate or aspartate residues, or on a vinyl sulfone activated dextran.

300. The MHC multimer according to claim 1 , wherein the multimerization domain is associated with the MHC peptide complex by a radical reaction.

301. The MHC multimer according to claim 1 , wherein the multimerization domain comprises one or more conjugated double bonds.

302. The MHC multimer according to claim 1 , wherein the MHC-peptide complex comprises one or more nucleophilic groups.

303. The MHC multimer according to claim 302, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -CN, -NH-NH₂.

304. The MHC multimer according to claim 1 , wherein the MHC-peptide complex comprises one or more electrophilic groups.

305. The MHC multimer according to claim 304, wherein the electrophilic group is selected from the group consisting of -COOH, -CHO, -CO, NHS-ester, tosyl- activated ester, and other activated esters, acid-anhydrides.

306. The MHC multimer according to claim 1 , wherein the MHC-peptide complex comprises one or more radicals.

307. The MHC multimer according to claim 1 , wherein the MHC-peptide complex comprises one or more conjugated double bonds.

308. The MHC multimer according to claim 53, wherein the multimerization domain comprising one or more beads further comprises a linker moiety

309. The MHC multimer according to claim 308, wherein the linker is a flexible linker.

310. The MHC multimer according to claim 308, wherein the linker is a rigid linker.

31 1. The MHC multimer according to claim 308, wherein the linker is a water- soluble linker.

312. The MHC multimer according to claim 308, wherein the linker is a cleavable linker.

313. The MHC multimer according to claim 312, wherein the cleavable linker is selected from linkers depicted in Figure 6 herein.

314. The MHC multimer according to claim 312, wherein the cleavable linker is cleavable at physiological conditions

315. The MHC multimer according to claim 1 , wherein the MHC-peptide complex is linked to at least one of the one or more multimerization domains by a non-covalent linker moiety.

316. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises natural dimerization.

317. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises protein-protein interactions.

318. The MHC multimer according to claim 317, wherein the protein-protein interactions comprises one or more Fos/Jun interactions.

319. The MHC multimer according to claim 317, wherein the protein-protein interactions comprises one or more Acid/Base coiled coil structure based interactions.

320. The MHC multimer according to claim 317, wherein the protein-protein interactions comprises one or more antibody/antigen interactions.

321. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises polynucleotide-polynucleotide interactions.

322. The MHC multimer according to claim 315 wherein the non-covalent linkage comprises protein-small molecule interactions.

323. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises combinations of non-covalent linker molecules.

324. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises synthetic molecule-synthetic molecule interactions.

325. The MHC multimer according to claim 316, wherein the natural dimerization comprises antigen-antibody pairs

326. The MHC multimer according to claim 316, wherein the natural dimerization comprises DNA-DNA interactions

327. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises natural interactions.

328. The MHC multimer according to claim 327, wherein the natural interaction comprises biotin and streptavidin.

329. The MHC multimer according to claim 53, wherein the bead is coated with streptavidin monomers, which in turn are associated with biotinylated MHC peptide complexes.

330. The MHC multimer according to claim 53, wherein the bead is coated with streptavidin tetramers each of which being independently associated with 0, 1 , 2, 3, or 4 biotinylated MHC complexes.

331. The MHC multimer according to claim 53, wherein the bead is coated with polysaccharide, such as a polysaccharide comprising dextran moieties.

332. The MHC multimer according to claim 327, wherein the natural interaction comprises the interaction of MHC complexes (comprising full-length polypeptide chains, including the transmembrane portion) with the cell membrane of for example dendritic cells. O

333. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises artificial interactions.

334. The MHC multimer according to claim 333, wherein the artificial interaction comprises His₆ tag interacting with Ni-NTA.

335. The MHC multimer according to claim 333, wherein the artificial interaction comprises PNA-PNA.

336. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises non-specific adsorption.

337. The MHC multimer according to claim 334, wherein the non-specific adsorption comprises adsorption of proteins onto surfaces.

338. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises the pentamer structure.

339. The MHC multimer according to claim 315, wherein the non-covalent linkage comprises interactions selected from the group streptavidin/biotin, avidin/biotin, antibody/antigen, DNA/DNA, DNA/PNA, DNA/RNA, PNA/PNA, LNA/DNA, leucine zipper e.g. Fos/Jun, IgG dimeric protein, IgM multivalent protein, acid/base coiled-coil helices, chelate/metal ion-bound chelate, streptavidin (SA) and avidin and derivatives thereof, biotin, immunoglobulins, antibodies (monoclonal, polyclonal, and recombinant), antibody fragments and derivatives thereof, leucine zipper domain of AP-1 (jun and fos), hexa-his (metal chelate moiety), hexa-hat GST (glutathione S-transferase) glutathione affinity, Calmodulin-binding peptide (CBP), Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, E2Tag, HA Epitope Tag, Myc Epitope, FLAG Epitope, AU1 and AU5 Epitopes, GIu-GIu Epitope, KT3 Epitope, IRS

Epitope, Btag Epitope, Protein Kinase-C Epitope, VSV Epitope, lectins that mediate binding to a diversity of compounds, including carbohydrates, lipids and proteins, e.g. Con A (Canavalia ensiformis) or WGA (wheat germ agglutinin) and tetranectin or Protein A or G (antibody affinity). o

340. The MHC multimer according to any of claims 1 to 339 further comprising one or more molecules with adjuvant effect.

341. The MHC multimer according to any of claims 1 to 339 further comprising one or more immune targets.

342. The MHC multimer according to any of claim 341 , wherein the one or more immune targets is an antigen.

343. The MHC multimer according to any of claims 1 to 342 further comprising one or more molecules with biological activity.

344. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises proteins such as MHC Class l-like proteins like MIC A, MIC B, CD1 d, HLA E, HLA F, HLA G, HLA H, ULBP-1 , ULBP-2, and ULBP-3.

345. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises co-stimulatory molecules such as CD2, CD3, CD4, CD5, CD8, CD9, CD27, CD28, CD30, CD69, CD134 (OX40), CD137 (4-

1 BB), CD147, CDw150 (SLAM), CD152 (CTLA-4), CD153 (CD30L), CD40L (CD154), NKG2D, ICOS, HVEM, HLA Class II, PD-1 , Fas (CD95), FasL expressed on T and/or NK cells, CD40, CD48, CD58, CD70, CD72, B7.1 (CD80), B7.2 (CD86), B7RP-1 , B7- H3, PD-L1 , PD-L2, CD134L, CD137L, ICOSL, LIGHT expressed on APC and/or tumour cells.

346. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises cell modulating molecules such as CD16, NKp30, NKp44, NKp46, NKp80, 2B4, KIR, LIR, CD94/NKG2A, CD94/NKG2C expressed on NK cells, IFN-alpha, IFN-beta, IFN-gamma, IL-1 , IL-2, IL-3, IL-4, IL-6,

IL-7, IL-8, IL-10, IL-1 1 , IL-12, IL-15, CSFs (colony-stimulating factors), vitamin D3, IL- 2 toxins, cyclosporin, FK-506, rapamycin, TGF-beta, clotrimazole, nitrendipine, and charybdotoxin.

347. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises accessory molecules such as LFA-1 , CDH a/18, CD54 (ICAM-1 ), CD106 (VCAM), and CD49a,b,c,d,e,f/CD29 (VLA-4).

348. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more non-classical MHC complexes and other MHC-like molecules such as protein products of MHC Ib and MHC Nb genes, β2m-associated cell-surface molecules, HLA-E, HLA-G, HLA-F, HLA-H, MIC A, MIC B, ULBP-1 , ULBP-2, ULBP-3, H2-M, H2-Q, H2-T, Rae, Non- classical MHC Il molecules, protein products of MHC Nb genes, HLA-DM, HLA-DO, H2-DM, H2-DO, and/or CD1 .

349. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more artificial molecules capable of binding specific TCRs such as antibodies that bind TCRs including full length antibodies of isotype IgG, IgM, IgE, IgA and truncated versions of these, antibody fragments like Fab fragments and scFv as well as antibodies of antibody fragments displayed on various supramolecular structures or solid supports, including filamentous phages, yeast, mammalian cells, fungi, artificial cells or micelles, and beads with various surface chemistries.

350. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more peptides that bind TCRs including one or more peptides composed of natural, non-natural and/or chemically modified amino acids with a length of 8-20 amino acid.

351. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more aptamers such as natural nucleic acids (e.g. RNA and DNA) or unnatural nucleic acids (e.g. PNA, LNA, morpholinos) capable of binding TCR, said aptamer molecules consist of natural or modified nucleotides in various lengths.

352. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more ankyrin repeat proteins or other repeat proteins. o

353. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more Avimers.

354. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises one or more small chemical molecules capable of binding TCR with a dissociation constant smaller than 10^'3 M.

355. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises adhesion molecules such as ICAM-1 ,

ICAM-2, GlyCAM-1 , CD34, anti-LFA-1 , anti-CD44, anti-beta7, chemokines, CXCR4, CCR5, anti-selectin L, anti-selectin E, and anti-selectin P.

356. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises toxic molecules selected from toxins, enzymes, antibodies, radioisotopes, chemiluminescent substances, bioluminescent substances, polymers, metal particles, and haptens, such as cyclophosphamide, methrotrexate, Azathioprine, mizoribine, 15-deoxuspergualin, neomycin, staurosporine, genestein, herbimycin A, Pseudomonas exotoxin A, saporin, Rituxan, Ricin, gemtuzumab ozogamicin, Shiga toxin, heavy metals like inorganic and organic mercurials, and FN18-CRM9, radioisotopes such as incorporated isotopes of iodide, cobalt, selenium, tritium, and phosphor, and haptens such as DNP, and digoxiginin.

357. The MHC multimer according to claim 343, wherein the one or more molecules with biological activity comprises antibodies such as monoclonal antibodies, polyclonal antibodies, recombinant antibodies, antibody derivatives or fragments thereof.

358. The MHC multimer according to any of claims 1 to 357 further comprising one or more molecules with a cytotoxic effect.

359. The MHC multimer according to any of claims 1 to 357 further comprising one or more molecules selected from the group consisting of enzymes, regulators of receptor activity, receptor ligands, immune potentiators, drugs, toxins, co-receptors, proteins, peptides, sugar moieties, lipid groups, nucleic acids including siRNA, nano particles, and small molecules.

360. The MHC multimer according to any of claims 1 to 357 further comprising one or more molecules selected from the group consisting of HIV gp120, HIV-GAG gp 27, HSP70, and MHC class Il proteins or peptides or combinations thereof.

361. The MHC multimer according to any of claims 1 to 360 comprising a plurality of identical or different multimerization domains linked by a multimerization domain linking moiety,

wherein at least one of said multimerization domains is associated with (a-b-P)_n, wherein n > 1 ,

wherein (a-b-P) is the MHC-peptide complex formed when the peptide P binds to the functional MHC protein.

362. The MHC multimer according to claim 361 , wherein the plurality of identical or different multimerization domains is in the range of from 2 to 100, suach as 2 to 5, 5 to 10, 10 to 20, 20 to 30, 30 to 40, 40 to 50, 50 to 60, 60 to 70, 70 to 80, 80 to 90, 90 to 100.

363. The MHC multimer according to claim 361 , wherein the MHC multimer comprises a first multimerization domain linked to a second multimerization domain.

364. The MHC multimer according to claim 363, wherein the first multimerization domain and the second multimerization domain is independently selected from the group consisting of multimerization domains cited in any of claims 4 to 75.

365. The MHC multimer according to claim 363, wherein the association is a covalent linkage so that one or more of the n MHC-peptide complexes is covalently linked to the first multimerization domains.

366. The MHC multimer according to claim 363, wherein the association is a non-covalent association so that one or more of the n MHC-peptide complexes is non-covalently associated with the first multimerization domain.

367. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more scaffolds.

368. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more carriers.

369. The MHC multimer according to claim 363, wherein the first multimerization domain comprises at least one scaffold and at least one carrier.

370. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more optionally substituted organic molecules.

371. The MHC multimer according to claim 370, wherein the optionally substituted organic molecule comprises one or more functionalized cyclic structures.

372. The MHC multimer according to claim 371 , wherein the one or more functionalized cyclic structures comprises one or more optionally substituted benzene rings.

373. The MHC multimer according to claim 370, wherein the optionally substituted organic molecule comprises a scaffold molecule comprising at least three reactive groups, or at least three sites suitable for non-covalent attachment.

374. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more biological cells, such as antigen presenting cells or dendritic cells. O

375. The MHC multimer according to claim 374, wherein the one or more biological cells are alive and mitotic active.

376. The MHC multimer according to claim 374, wherein the one or more biological cells are alive and mitotic inactive e.g. as a result of irradiation and/or chemically treatment.

377. The MHC multimer according to claim 374, wherein the one or more biological cells are dead.

378. The MHC multimer according to claim 374, wherein the one or more biological cells have a natural expression of MHC (i.e. not stimulated).

379. The MHC multimer according to claim 374, wherein the one or more biological cells have to be induced/stimulated by e.g. lnf-γ to express MHC.

380. The MHC multimer according to claim 374, wherein the one or more biological cells are macrophages.

381. The MHC multimer according to claim 374, wherein the one or more biological cells are Kupfer cells.

382. The MHC multimer according to claim 374, wherein the one or more biological cells are Langerhans cells.

383. The MHC multimer according to claim 374, wherein the one or more biological cells are B-cells.

384. The MHC multimer according to claim 374, wherein the one or more biological cells are MHC expressing cells.

385. The MHC multimer according to claim 374, wherein the one or more biological cells are one or more transfected cells expressing MHC.

.

386. The MHC multimer according to claim 374, wherein the one or more biological cells are one or more hybridoma cells expressing MHC.

387. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more cell-like structures.

388. The MHC multimer according to claim 387, wherein the one or more cell- like structures comprises one or more membrane-based structures carrying MHC- peptide complexes in their membranes such as micelles, liposomes, and other structures of membranes, and phages such as filamentous phages.

389. The MHC multimer according to claim 363 wherein the first multimerization domain comprises one or more membranes.

390. The MHC multimer according to claim 389, wherein the one or more membranes comprises liposomes or micelles.

391. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more polymers.

392. The MHC multimer according to claim 391 , wherein the one or more polymers are selected from the group consisting of the group consisting of polysaccharides.

393. The MHC multimer according to claim 392, wherein the polysaccharide comprises one or more dextran moieties.

394. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more entities selected from the group consisting of an IgG domain, a coiled-coil polypeptide structure, a DNA duplex, a nucleic acid duplex, PNA-PNA, PNA-DNA, DNA-RNA.

395. The MHC multimer according to claim 363, wherein the first multimerization domain comprises an avidin, such as streptavidin.

396. The MHC multimer according to claim 363, wherein the first multimerization domain comprises an antibody.

397. The MHC multimer according to claim 396, wherein the antibody is selected from the group consisting of polyclonal antibody, monoclonal antibody, IgA,

IgG, IgM, IgD, IgE, IgGI , lgG2, lgG3, lgG4, IgAI , lgA2, IgMI , lgM2, humanized antibody, humanized monoclonal antibody, chimeric antibody, mouse antibody, rat antibody, rabbit antibody, human antibody, camel antibody, sheep antibody, engineered human antibody, epitope-focused antibody, agonist antibody, antagonist antibody, neutralizing antibody, naturally-occurring antibody, isolated antibody, monovalent antibody, bispecific antibody, trispecific antibody, multispecific antibody, heteroconjugate antibody, immunoconjugates, immunoliposomes, labeled antibody, antibody fragment, domain antibody, nanobody, minibody, maxibody, diabody, fusion antibody.

398. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more small organic scaffold molecules.

399. The MHC multimer according to claim 363, wherein the first multimerization comprises one or more further polypeptides in addition to a and b.

400. The MHC multimer according to claim 363, wherein the first multimerization comprises one or more protein complexes.

401. The MHC multimer according to claim 363, wherein the first multimerization comprises one or more beads.

402. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more compounds selected from the group consisting of agarose, sepharose, resin beads, glass beads, pore-glass beads, glass particles coated with a hydrophobic polymer, chitosan-coated beads, SH beads, latex beads, spherical latex beads, allele-type beads, SPA bead, PEG-based resins, PEG-coated bead, PEG-encapsulated bead, polystyrene beads, magnetic polystyrene beads, glutathione agarose beads, magnetic bead, paramagnetic beads, protein A and/or protein G sepharose beads, activated carboxylic acid bead, macroscopic beads, microscopic beads, insoluble resin beads, silica-based resins, cellulosic resins, cross-linked agarose beads, polystyrene beads, cross-linked polyacrylamide resins, beads with iron cores, metal beads, dynabeads, Polymethylmethacrylate beads activated with NHS, streptavidin-agarose beads, polypropylene, polyethylene, dextran, nylon, amylases, natural and modified celluloses, nitrocellulose, polyacrylamides, gabbros, magnetite, polymers, oligomers, non-repeating moieties, polyethylene glycol (PEG), monomethoxy-PEG, mono-(d- Cio)alkoxy-PEG, aryloxy-PEG, poly-(N-vinyl pyrrolidone)PEG, tresyl monomethoxy PEG, PEG propionaldehyde, bis-succinimidyl carbonate PEG, polystyrene bead crosslinked with divinylbenzene, propylene glycol homopolymers, a polypropylene oxide/ethylene oxide co-polymer, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, dextran, aminodextran, carbohydrate-based polymers, cross-linked dextran beads, polysaccharide beads, polycarbamate beads, divinyl sulfone activated polysaccharide, polystyrene beads that have been functionalized with tosyl-activated esters, magnetic polystyrene beads functionalized with tosyl-activated esters, streptavidin beads, streptaivdin-monomer coated beads, streptaivdin-tetramer coated beads, Streptavidin Coated Compel Magnetic beads, avidin coated beads, dextramer coated beads, divinyl sulfone-activated dextran, Carboxylate-modified bead, amine- modified beads, antibody coated beads, cellulose beads, grafted co-poly beads, poly- acrylamide beads, dimethylacrylamide beads optionally crosslinked with N-N'-bis- acryloylethylenediamine, hollow fiber membranes, fluorescent beads, collagen- agarose beads, gelatin beads, collagen-gelatin beads, collagen-fibronectin-gelatin beads, collagen beads, chitosan beads, collagen-chitosan beads, protein-based beads, hydrogel beads, hemicellulose, alkyl cellulose, hydroxyalkyl cellulose, carboxymethylcellulose, sulfoethylcellulose, starch, xylan, amylopectine, chondroitin, hyarulonate, heparin, guar, xanthan, mannan, galactomannan, chitin and chitosan.

403. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a dimerization domain.

404. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a trimerization domain.

405. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a tetramerization domain. O

406. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a pentamerization domain.

407. The MHC multimer according to claim 406, wherein the pentamerization domain comprises a coiled-coil polypeptide structure.

408. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a hexamerization domain.

409. The MHC multimer according to claim 408, wherein the hexamerization domain comprises three IgG domains.

410. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a polymer structure to which is attached one or more scaffolds.

41 1. The MHC multimer according to claim 410, wherein the polymer structure comprises a polysaccharide.

412. The MHC multimer according to claim 41 1 , wherein the polysaccharide comprises one or more dextran moieties.

413. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a polyamide and/or a polyethylene glycol and/or a polysaccharide and/or a sepharose.

414. The MHC multimer according to claim 363, wherein the first multimerization domain comprises a carboxy methyl dextran and/or a dextran polyaldehyde and/or a carboxymethyl dextran lactone and/or or a cyclodextrin.

415. The MHC multimer according to claim 363, wherein one or more labels is covalently attached to the first multimerization domain. Ό

416. The MHC multimer according to claim 363, wherein one or more labels is non-covalently attached to the first multimerization domain.

417. The MHC multimer according to claim 416, wherein the one or more labels is non-covalently attached to an antibody in the first mutimerization domain.

418. The MHC multimer according claim 415, wherein the one or more labels is covalently attached to an antibody in the first multimerization domain.

419. The MHC multimer according claim 416, wherein the one or more labels is non-covalently attached to an aptamer in the first mutimerization domain.

420. The MHC multimer according claim 415, wherein the one or more labels is covalently attached to an aptamer in the first mutimerization domain.

421 . The MHC multimer according claim 416, wherein the one or more labels is non-covalently attached to a molecule in the first mutimerization domain.

422. The MHC multimer according claim 415, wherein the one or more labels is covalently attached to a molecule in the first mutimerization domain.

423. The MHC multimer according claim 416, wherein the one or more labels is non-covalently attached to a protein in the first mutimerization domain.

424. The MHC multimer according claim 415, wherein the one or more labels is covalently attached to a protein in the first mutimerization domain.

425. The MHC multimer according claim 416, wherein the one or more labels is non-covalently attached to a sugar residue in the first mutimerization domain.

426. The MHC multimer according claim 415, wherein the one or more labels is covalently attached to a sugar residue in the first mutimerization domain.

427. The MHC multimer according claim 416, wherein the one or more labels is non-covalently attached to a DNA in the first mutimerization domain. i l

428. The MHC multimer according claim 415, wherein the one or more labels is covalently attached to a DNA in the first mutimerization domain.

429. The MHC multimer according to any of the claims 415 to 428, wherein the attachment is directly between reactive groups in the labelling molecule and reactive groups in the marker molecule.

430. The MHC multimer according to any of the claims 415 to 428, wherein the attachment is through a linker connecting labelling molecule and marker.

431. The MHC multimer according to any of the claims 415 to 430, wherein one label is used.

432. The MHC multimer according to any of the claims 415 to 430, wherein more than one label is used.

433. The MHC multimer according to claim 432, wherein the more than one labels are all identical.

434. The MHC multimer according to claim 432, wherein at least two labels are different.

435. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a fluorophore.

436. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of selected from the group fluorescein isothiocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde and fluorescamine.

437. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 2-(4'- maleimidylanilino)naphthalene-6- sulfonic acid, sodium salt O o

438. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 5-((((2- iodoacetyl)amino)ethyl)amino) naphthalene-1 -sulfonic acid

439. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Pyrene-1 -butanoic acid

440. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of AlexaFIuor 350 (7-amino- 6-sulfonic acid-4-methyl coumarin-3-acetic acid

441. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of AMCA (7-amino-4-methyl coumarin-3-acetic acid

442. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 7-hydroxy-4-methyl coumarin-3-acetic acid.

443. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Marina Blue (6,8-difluoro- 7-hydroxy-4-methyl coumarin-3-acetic acid.

444. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 7-dimethylamino- coumarin-4-acetic acid.

445. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Fluorescamin-N-butyl amine adduct.

446. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 7-hydroxy-coumarine-3- carboxylic acid.

447. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of CascadeBIue (pyrene- trisulphonic acid acetyl azide.

448. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Cascade Yellow.

449. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Pacific Blue (6,8 difluoro- 7-hydroxy coumarin-3-carboxylic acid.

450. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 7-diethylamino-coumarin- 3-carboxylic acid.

451. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of N-(((4- azidobenzoyl)amino)ethyl)- 4-amino-3,6-disulfo-1 ,8-naphthalimide, dipotassium salt.

452. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Alexa Fluor 430.

453. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 3-perylenedodecanoic acid.

454. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 8-hydroxypyrene-1 ,3,6- trisulfonic acid, trisodium salt.

455. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 12-(N-(7-nitrobenz-2-oxa- 1 ,3- diazol-4-yl)amino)dodecanoic acid. o

456. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of N,N'-dimethyl-N- (iodoacetyl)-N'-(7-nitrobenz-2- oxa-1 ,3-diazol-4-yl)ethylenediamine.

457. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Oregon Green 488 (difluoro carboxy fluorescein).

458. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of 5- iodoacetamidofluorescein.

459. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of propidium iodide-DNA adduct.

460. The MHC multimer according to claim 435, wherein the one or more fluorophore label are selected from the group consisting of Carboxy fluorescein.

461 . The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a fluorescent label.

462. The MHC multimer according to claim 461 , wherein the one or more fluorescent label is a simple fluorescent label.

463. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group Fluor dyes, Pacific Blue™, Pacific Orange™, Cascade Yellow™.

464. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group AlexaFluor@(AF), AF405, AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750, AF800. o

465. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group Quantum Dot based dyes, QDot® Nanocrystals (Invitrogen, MolecularProbs), Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800.

466. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group DyLight™ Dyes (Pierce) (DL); DL549, DL649, DL680, DL800.

467. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group Fluorescein (Flu) or any derivate of that, such as FITC.

468. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group Cy-Dyes, Cy2, Cy3, Cy3.5, Cy5,

Cy5.5, Cy7.

469. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group Fluorescent Proteins, RPE, PerCp, APC, Green fluorescent proteins; GFP and GFP derived mutant proteins;

BFP,CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine, mStrawberry, mCherry.

470. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group Tandem dyes, RPE-Cy5, RPE-

471. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group multi fluorochrome assemblies,

472. The MHC multimer according to claim 462, wherein the one or more simple fluorescent label is selected from the group ionophors; ion chelating fluorescent props, props that change wavelength when binding a specific ion, such as Calcium, props that change intensity when binding to a specific ion, such as Calcium.

473. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is capable of absorption of light

474. The MHC multimer according to claim 473, wherein the one or more labels capable of absorption of light is a chromophore.

475. The MHC multimer according to claim 473, wherein the one or more labels capable of absorption of light is a dye.

476. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is capable of emission of light after excitation

477. The MHC multimer according to claim 476, wherein the one or more labels capable of emission of light is one or more fluorochromes.

478. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the AlexaFluor@(AF) family, which include AF®350, AF405, AF430, AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750 and AF800

479. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the Quantum Dot (Qdot®) based dye family, which include Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800

480. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the DyLight™ Dyes (DL) family, which include DL549, DL649, DL680, DL800

481. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Small fluorescing dyes, which include FITC, Pacific Blue™, Pacific Orange™, Cascade Yellow™, Marina blue™, DSred, DSred-2, 7-AAD, TO-Pro-3.

482. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Cy-Dyes, which include Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7

483. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Phycobili Proteins, which include R- Phycoerythrin (RPE), PerCP, Allophycocyanin (APC), B-Phycoerythrin, C- Phycocyanin.

484. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Fluorescent Proteins, which include (E)GFP and GFP ((enhanced) green fluorescent protein) derived mutant proteins; BFP, CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine.

485. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Tandem dyes with RPE, which include RPE-Cy5, RPE-Cy5.5, RPE-Cy7, RPE-AlexaFluor® tandem conjugates; RPE- Alexa610, RPE-TxRed.

486. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Tandem dyes with APC, which include APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5, APC-Cy5.5.

487. The MHC multimer according to claim 477, wherein the one or more fluorochrome is selected from the family of Calcium dyes, which include Indo-1 -Ca²⁺ lndo-2-Ca²⁺.

488. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is capable of reflection of light

489. The MHC multimer according to claim 488, wherein the one or more labels capable of reflection of light comprises gold

490. The MHC multimer according to claim 488, wherein the one or more labels capable of reflection of light comprises plastic

491. The MHC multimer according to claim 488, wherein the one or more labels capable of reflection of light comprises glass

492. The MHC multimer according to claim 488, wherein the one or more labels capable of reflection of light comprises polystyrene

493. The MHC multimer according to claim 488, wherein the one or more labels capable of reflection of light comprises pollen

494. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a chemiluminescent label.

495. The MHC multimer according to claim 494, wherein the chemiluminescent labels is selected from the group luminol, isoluminol, theromatic acridinium ester, imidazole, acridinium salt and oxalate ester.

496. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a bioluminescent label.

497. The MHC multimer according to claim 496, wherein the bioluminescent labels is selected from the group luciferin, luciferase and aequorin.

498. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a radioactive label.

499. The MHC multimer according to claim 498, wherein the one or more radioactive labels is a radionuclide. O

500. The MHC multimer according to claim 498, wherein the one or more radioactive labels is an isotope.

501. The MHC multimer according to claim 498, wherein the one or more radioactive labels comprises α rays.

502. The MHC multimer according to claim 498, wherein the one or more radioactive labels comprises β rays.

503. The MHC multimer according to claim 498, wherein the one or more radioactive labels comprises y rays.

504. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is detectable by NMR (nuclear magnetic resonance form paramagnetic molecules)

505. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is an enzyme label.

506. The MHC multimer according to claim 505, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, producing a light signal (chemi-luminescence).

507. The MHC multimer according to claim 505, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, resulting in precipitation of chromophor dyes.

508. The MHC multimer according to claim 505, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, resulting in precipitates that can be detected by an additional layer of detection molecules.

509. The MHC multimer according to claim 505, wherein the enzyme label is selected from the group peroxidases, malate dehydrogenase, staphylococcal nuclease, delta-5-steroid isomerase, yeast alcohol dehydrogenase, alpha- o

glycerophosphate, dehydrogenase, triose phosphate isomerase, horseradish peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta- galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase and acetylcholinesterase.

510. The MHC multimer according to claim 505, wherein the enzyme label is horseradish peroxidase.

51 1. The MHC multimer according to claim 505, wherein the enzyme label is horseradish peroxidase and the substrate is diaminobenzidine (DAB).

512. The MHC multimer according to claim 505, wherein the enzyme label is horseradish peroxidase and the substrate is 3-amino-9-ethyl-carbazole (AEC+).

513. The MHC multimer according to claim 505, wherein the enzyme label is horseradish peroxidase and the substrate is biotinyl tyramide.

514. The MHC multimer according to claim 505, wherein the enzyme label is horseradish peroxidase and the substrate is fluorescein tyramide.

515. The MHC multimer according to claim 505, wherein the enzyme label is alkaline phosphatase.

516. The MHC multimer according to claim 505, wherein the enzyme label is alkaline phosphatase and the substrate is Fast red dye.

517. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a ionophore or chelating chemical compound binding to specific ions such as Ca²⁺

518. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a lanthanide.

519. The MHC multimer according to claim 518, wherein the lanthanide comprises fluorescence.

520. The MHC multimer according to claim 518, wherein the lanthanide comprises Phosphorescence.

521. The MHC multimer according to claim 518, wherein the lanthanide is paramagnetic.

522. The MHC multimer according to any of claims 415 to 434, wherein the one or more labels is a DNA fluorescing stain.

523. The MHC multimer according to claim 522, wherein the DNA fluorescing stain is Propidium iodide.

524. The MHC multimer according to claim 522, wherein the DNA fluorescing stain is Hoechst stain.

525. The MHC multimer according to claim 522, wherein the DNA fluorescing stain is DAPI.

526. The MHC multimer according to claim 522, wherein the DNA fluorescing stain is AMC.

527. The MHC multimer according to claim 522, wherein the DNA fluorescing stain is DraQ5™

528. The MHC multimer according to claim 522, wherein the DNA fluorescing stain is Acridine orange.

529. The MHC multimer according to claim 361 , wherein the MHC-peptide complex (a-b-P) is attached to the first multimerization domain comprising an avidin or streptavidin via a linkage comprising a biotin moiety.

530. The MHC multimer according to any of claims 361 to 529, wherein the MHC-peptide complex is linked to the first multimerization domain by a first linker moiety. o

531. The MHC multimer according to claim 530, wherein the MHC-peptide complex is linked to the first multimerization domain by a covalent linker moiety.

532. The MHC multimer according to claims 530 and 531 , wherein the association of the first multimerization domain and at least one MHC-peptide complexes is formed by a binding entity X attached to, or being part of, the first multimerization domain, and a binding entity Y attached to, or being part of at least one of the MHC-peptide complexes.

533. The MHC multimer according to claims 530 and 531 , wherein the linker moiety linking the first multimerization domain and the MHC-peptide complex comprises the linker moiety XY, wherein the linker moiety XY results from a reaction of the moiety X comprising one or more reactive groups and the moiety Y comprising one or more reactive groups, wherein at least some of said reactive groups are capable of reacting with each other.

534. The MHC multimer according to any of claims 532 and 533, wherein the moiety X comprises a nucleophilic group.

535. The MHC multimer according to claim 534, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -NH-NH₂.

536. The MHC multimer according to any of claims 532 and 533, wherein the moiety Y comprises an electrophilic group.

537. The MHC multimer according to claim 536, wherein the electrophilic group is selected from the group consisting of CHO, COOH and CO.

538. The MHC multimer according to claims 532 and 533, wherein at least one reactive group on one of the moieties X and Y comprises a radical capable of reacting with a reactive group forming part of the other moiety.

539. The MHC multimer according to claims 532 and 533, wherein X and Y comprises reactive groups natively associated with the first multimerization domain and/or the MHC-peptide complexes.

540. The MHC multimer according to claims 532 and 533, wherein X and Y comprises reactive groups not natively associated with the first multimerization domain and/or the MHC-peptide complex.

541. The MHC multimer according to claims 530 and 531 , wherein the linker moiety forms a covalent link between the first multimerization domain and at least one of the MHC-peptide complexes.

542. The MHC multimer according to claims 532 and 533, wherein the reactive groups of MHC-peptide complexes include amino acid side chains selected from the group consisting of -NH₂, -OH, -SH, and -NH-

543. The MHC multimer according to claims 532 and 533, wherein the reactive groups of the first multimerization domain include hydroxyls of polysaccharides such as dextrans

544. The MHC multimer according to claims 532 and 533, wherein the reactive groups of the first multimerization domain selected from the group consisting of amino acid side chains comprising -NH₂, -OH, -SH, and -NH- of polypeptides

545. The MHC multimer according to claims 530 and 531 , wherein one of the polypeptides of the MHC-peptide complex is linked by a protein fusion to the first multimerization domain

546. The MHC multimer according to claims 530 and 531 , wherein one of the polypeptides of the MHC-peptide complex is linked by a protein fusion to the first multimerization domain, wherein an acyl group and an amino group react to form an amide bond

547. The MHC multimer according to claim 361 , wherein one of the polypeptides of the MHC-peptide complex is linked by non-native reactive groups to the first multimerization domain.

548. The MHC multimer according to claim 361 , wherein the reactive groups include reactive groups that are attached to the first multimerization domain through association of a linker molecule comprising the reactive group.

549. The MHC multimer according to claim 361 , wherein the reactive groups include reactive groups that are attached to the MHC-peptide complex through association of a linker molecule comprising the reactive group.

550. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more nucleophilic groups

551. The MHC multimer according to claim 550, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -CN, -NH-NH₂

552. The MHC multimer according to claim 363, wherein the first multimerization domain is selected from the group consisting of polysaccharides, polypeptides comprising e.g. lysine, serine, and cysteine.

553. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more electrophilic groups.

554. The MHC multimer according to claim 553, wherein the electrophilic group is selected from the group consisting of -COOH, -CHO, -CO, NHS-ester, tosyl- activated ester, and other activated esters, acid-anhydrides.

555. The MHC multimer according to claim 363, wherein the first multimerization domain is selected from the group consisting of polypeptides comprising e.g. glutamate and aspartate, or vinyl sulfone activated dextran.

556. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more radicals.

557. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more conjugated double bonds.

558. The MHC multimer according to claim 363, wherein the first multimerization domain comprises one or more beads, further comprising a linker moiety

559. The MHC multimer according to any of the claims 530 to 558, wherein the linker is a flexible linker.

560. The MHC multimer according to any of the claims 530 to 558, wherein the linker is a rigid linker.

561. The MHC multimer according to any of the claims 530 to 558, wherein the linker is a water-soluble linker.

562. The MHC multimer according to any of the claims 530 to 558, wherein the linker is a cleavable linker.

563. The MHC multimer according to claim 562, wherein the cleavable linker is selected from linkers depicted in Figure 6 herein.

564. The MHC multimer according to claim 562, wherein the cleavable linker is cleavable at physiological conditions.

565. The MHC multimer according to claims 530 or 531 , wherein the MHC- peptide complex is linked to the first multimerization domain by a non-covalent linker moiety.

566. The MHC multimer according to claim 565, wherein the non-covalent linkage comprises natural dimerization

567. The MHC multimer according to claim 566, wherein the natural dimerization comprises antigen-antibody pairs

568. The MHC multimer according to claim 566, wherein the natural dimerization comprises DNA-DNA interactions

569. The MHC multimer according to claim 565, wherein the non-covalent linkage comprises natural interactions

570. The MHC multimer according to claim 569, wherein the natural interaction comprises biotin and streptavidin

571. The MHC multimer according to claim 401 , wherein the bead is coated with streptavidin monomers, which in turn are associated with biotinylated MHC complexes

572. The MHC multimer according to claim 401 , wherein the bead is coated with streptavidin tetramers, each of which being independently associated with 0, 1 , 2, 3, or 4 biotinylated MHC complexes

573. The MHC multimer according to claim 401 , wherein the bead is coated with a polysaccharide, such as a polysaccharide comprising dextran moieties.

574. The MHC multimer according to claim 569, wherein the natural interaction comprises the interaction of MHC complexes (comprising full-length polypeptide chains, including the transmembrane portion) with the cell membrane of for example dendritic cells

575. The MHC multimer according to claim 565, wherein the non-covalent linkage comprises artificial interactions

576. The MHC multimer according to claim 575, wherein the artificial interaction comprises His₆ tag interacting with Ni-NTA

577. The MHC multimer according to claim 575, wherein the artificial interaction comprises PNA-PNA

578. The MHC multimer according to claim 565, wherein the non-covalent linkage comprises non-specific adsorption

579. The MHC multimer according to claim 578, wherein the non-specific adsorption comprises adsorption of proteins onto surfaces

580. The MHC multimer according to claim 565, wherein the non-covalent linkage comprises the pentamer structure

581. The MHC multimer according to claim 565, wherein the non-covalent linkage comprises interactions selected from the group streptavidin/biotin, avidin/biotin, antibody/antigen, DNA/DNA, DNA/PNA, DNA/RNA, PNA/PNA, LNA/DNA, leucine zipper e.g. Fos/Jun, IgG dimeric protein, IgM multivalent protein, acid/base coiled-coil helices, chelate/metal ion-bound chelate, streptavidin (SA) and avidin and derivatives thereof, biotin, immunoglobulins, antibodies (monoclonal, polyclonal, and recombinant), antibody fragments and derivatives thereof, leucine zipper domain of AP-1 (jun and fos), hexa-his (metal chelate moiety), hexa-hat GST (glutathione S-transferase) glutathione affinity, Calmodulin-binding peptide (CBP), Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, E2Tag, HA Epitope Tag, Myc

582. The MHC multimer according to claim 365, wherein the association is a covalent linkage so that one or more of the n MHC-peptide complexes is covalently linked to the second multimerization domains.

583. The MHC multimer according to claim 365 wherein the association is a non-covalent association so that one or more of the n MHC-peptide complexes is non-covalently associated with the second multimerization domain.

584. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more scaffolds.

585. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more carriers.

586. The MHC multimer according to claim 363, wherein the second multimerization domain comprises at least one scaffold and at least one carrier.

587. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more optionally substituted organic molecules.

588. The MHC multimer according to claim 587, wherein the optionally substituted organic molecule comprises one or more functionalized cyclic structures.

589. The MHC multimer according to claim 588 wherein the one or more functionalized cyclic structures comprises one or more benzene rings.

590. The MHC multimer according to claim 587, wherein the optionally substituted organic molecule comprises a scaffold molecule comprising at least three reactive groups, or at least three sites suitable for non-covalent attachment.

591. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more biological cells, such as antigen presenting cells or dendritic cells.

592. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more membranes.

593. The MHC multimer according to claim 592, wherein the one or more membranes comprises liposomes or micelles.

594. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more polymers. o

595. The MHC multimer according to claim 594 wherein the one or more polymers are selected from the group consisting of the group consisting of polysaccharides.

596. The MHC multimer according to claim 595, wherein the polysaccharide comprises one or more dextran moieties.

597. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more entities selected from the group consisting of an IgG domain, a coiled-coil polypeptide structure, a DNA duplex, a nucleic acid duplex, PNA-PNA, PNA-DNA, DNA-RNA.

598. The MHC multimer according to claim 363, wherein the second multimerization domain comprises an avidin, such as streptavidin.

599. The MHC multimer according to claim 363, wherein the second multimerization domain comprises an antibody.

600. The MHC multimer according to claim 599, wherein the antibody is selected from the group consisting of polyclonal antibody, monoclonal antibody, IgA, IgG, IgM, IgD, IgE, IgGI , lgG2, lgG3, lgG4, IgAI , lgA2, IgMI , lgM2, humanized antibody, humanized monoclonal antibody, chimeric antibody, mouse antibody, rat antibody, rabbit antibody, human antibody, camel antibody, sheep antibody, engineered human antibody, epitope-focused antibody, agonist antibody, antagonist antibody, neutralizing antibody, naturally-occurring antibody, isolated antibody, monovalent antibody, bispecific antibody, trispecific antibody, multispecific antibody, heteroconjugate antibody, immunoconjugates, immunoliposomes, labeled antibody, antibody fragment, domain antibody, nanobody, minibody, maxibody, diabody, fusion antibody.

601. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more small organic scaffold molecules.

602. The MHC multimer according to claim 363, wherein the second multimerization comprises one or more further polypeptides in addition to a and b.

603. The MHC multimer according to claim 363, wherein the second multimerization comprises one or more protein complexes.

604. The MHC multimer according to claim 363, wherein the second multimerization comprises one or more beads

605. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more compounds selected from the group consisting of agarose, sepharose, resin beads, glass beads, pore-glass beads, glass particles coated with a hydrophobic polymer, chitosan-coated beads, SH beads, latex beads , spherical latex beads, allele-type beads, SPA bead, PEG-based resins, PEG-coated bead, PEG-encapsulated bead, polystyrene beads, magnetic polystyrene beads, glutathione agarose beads, magnetic bead, paramagnetic beads, protein A and/or protein G sepharose beads, activated carboxylic acid bead, macroscopic beads, microscopic beads, insoluble resin beads, silica-based resins, cellulosic resins, cross-linked agarose beads, polystyrene beads, cross-linked polyacrylamide resins, beads with iron cores, metal beads, dynabeads,

Polymethylmethacrylate beads activated with NHS, streptavidin-agarose beads, polypropylene, polyethylene, dextran, nylon, amylases, natural and modified celluloses, nitrocellulose, polyacrylamides, gabbros, magnetite, polymers, oligomers, non-repeating moieties, polyethylene glycol (PEG), monomethoxy-PEG, mono-(d- Cio)alkoxy-PEG, aryloxy-PEG, poly-(N-vinyl pyrrolidone)PEG, tresyl monomethoxy

PEG, PEG propionaldehyde, bis-succinimidyl carbonate PEG, polystyrene bead crosslinked with divinylbenzene, propylene glycol homopolymers, a polypropylene oxide/ethylene oxide co-polymer, polyoxyethylated polyols (e.g., glycerol), polyvinyl alcohol, dextran, aminodextran, carbohydrate-based polymers, cross-linked dextran beads, polysaccharide beads, polycarbamate beads, divinyl sulfone activated polysaccharide, polystyrene beads that have been functionalized with tosyl-activated esters, magnetic polystyrene beads functionalized with tosyl-activated esters, streptavidin beads, streptaivdin-monomer coated beads, streptaivdin-tetramer coated beads, Streptavidin Coated Compel Magnetic beads, avidin coated beads, dextramer coated beads, divinyl sulfone-activated dextran, Carboxylate-modified bead, amine- modified beads, antibody coated beads, cellulose beads, grafted co-poly beads, poly- acrylamide beads, dimethylacrylamide beads optionally crosslinked with N-N'-bis- acryloylethylenediamine, hollow fiber membranes, fluorescent beads, collagen- agarose beads, gelatin beads, collagen-gelatin beads, collagen-fibronectin-gelatin beads, collagen beads, chitosan beads, collagen-chitosan beads, protein-based beads, hydrogel beads, hemicellulose, alkyl cellulose, hydroxyalkyl cellulose, carboxymethylcellulose, sulfoethylcellulose, starch, xylan, amylopectine, chondroitin, hyarulonate, heparin, guar, xanthan, mannan, galactomannan, chitin and chitosan.

606. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a dimerization domain.

607. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a trimerization domain.

608. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a tetramerization domain.

609. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a pentamerization domain.

610. The MHC multimer according to claim 609, wherein the pentamerization domain comprises a coiled-coil polypeptide structure.

61 1. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a hexamerization domain.

612. The MHC multimer according to claim 61 1 , wherein the hexamerization domain comprises three IgG domains.

613. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a polymer structure to which is attached one or more scaffolds.

614. The MHC multimer according to claim 613, wherein the polymer structure comprises a polysaccharide.

615. The MHC multimer according to claim 614, wherein the polysaccharide comprises one or more dextran moieties.

616. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a polyamide and/or a polyethylene glycol and/or a polysaccharide and/or a sepharose.

617. The MHC multimer according to claim 363, wherein the second multimerization domain comprises a carboxy methyl dextran and/or a dextran polyaldehyde and/or a carboxymethyl dextran lactone and/or a cyclodextrin.

618. The MHC multimer according to claim 363, wherein one or more labels is covalently attached to the second multimerization domain.

619. The MHC multimer according to claim 363, wherein one or more labels is non-covalently attached to the second multimerization domain.

620. The MHC multimer according to claim 619, wherein the one or more labels is non-covalently attached to an antibody in the second mutimerization domain.

621. The MHC multimer according to claim 618, wherein the one or more labels is covalently attached to an antibody in the second mutimerization domain.

622. The MHC multimer according to claim 619, wherein the one or more labels is non-covalently attached to an aptamer in the second mutimerization domain.

623. The MHC multimer according to claim 618, wherein the one or more labels is covalently attached to an aptamer in the second mutimerization domain.

624. The MHC multimer according to claim 619, wherein the one or more labels is non-covalently attached to a molecule in the second mutimerization domain.

625. The MHC multimer according to claim 618, wherein the one or more labels is covalently attached to a molecule in the second mutimerization domain.

626. The MHC multimer according to claim 619, wherein the one or more labels is non-covalently attached to a protein in the second mutimerization domain.

627. The MHC multimer according to claim 618, wherein the one or more labels is covalently attached to a protein in the second mutimerization domain.

628. The MHC multimer according to claim 619, wherein the one or more labels is non-covalently attached to a sugar residue in the second mutimerization domain.

629. The MHC multimer according to claim 618, wherein the one or more labels is covalently attached to a sugar residue in the second mutimerization domain.

630. The MHC multimer according to claim 619, wherein the one or more labels is non-covalently attached to a DNA in the second mutimerization domain.

631. The MHC multimer according to claim 618, wherein the one or more labels is covalently attached to a DNA in the second mutimerization domain.

632. The MHC multimer according to any of the claims 618 to 631 , wherein the attachment is directly between reactive groups in the labelling molecule and reactive groups in the marker molecule.

633. The MHC multimer according to any of the claims 618 to 631 , wherein the attachment is through a linker connecting labelling molecule and marker.

634. The MHC multimer according to any of the claims 618 to 631 , wherein one label is used.

635. The MHC multimer according to any of the claims 618 to 631 , wherein more than one label is used.

636. The MHC multimer according to claim 635, wherein the more than one label are all identical.

637. The MHC multimer according to claim 635, wherein at least two labels are different.

638. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a fluorophore.

639. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of selected from the group fluorescein isothiocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde and fluorescamine.

640. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 2-(4'- maleimidylanilino)naphthalene-6- sulfonic acid, sodium salt

641. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 5-((((2- iodoacetyl)amino)ethyl)amino) naphthalene-1 -sulfonic acid

642. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Pyrene-1 -butanoic acid

643. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of AlexaFIuor 350 (7-amino- 6-sulfonic acid-4-methyl coumarin-3-acetic acid

644. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of AMCA (7-amino-4-methyl coumarin-3-acetic acid

645. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 7-hydroxy-4-methyl coumarin-3-acetic acid

646. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Marina Blue (6,8-difluoro- 7-hydroxy-4-methyl coumarin-3-acetic acid

647. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 7-dimethylamino- coumarin-4-acetic acid

648. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Fluorescamin-N-butyl amine adduct

649. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 7-hydroxy-coumarine-3- carboxylic acid

650. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of CascadeBIue (pyrene- trisulphonic acid acetyl azide

651 . The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Cascade Yellow

652. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Pacific Blue (6,8 difluoro- 7-hydroxy coumarin-3-carboxylic acid

653. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 7-diethylamino-coumarin- 3-carboxylic acid

654. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of N-(((4- azidobenzoyl)amino)ethyl)- 4-amino-3,6-disulfo-1 ,8-naphthalimide, dipotassium salt

655. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Alexa Fluor 430

656. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 3-perylenedodecanoic acid

657. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 8-hydroxypyrene-1 ,3,6- trisulfonic acid, trisodium salt

658. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 12-(N-(7-nitrobenz-2-oxa- 1 ,3- diazol-4-yl)amino)dodecanoic acid

659. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of N,N'-dimethyl-N- (iodoacetyl)-N'-(7-nitrobenz-2- oxa-1 ,3-diazol-4-yl)ethylenediamine

660. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Oregon Green 488

(difluoro carboxy fluorescein)

661. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of 5- iodoacetamidofluorescein

662. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of propidium iodide-DNA adduct

663. The MHC multimer according to claim 638, wherein the one or more fluorophore label are selected from the group consisting of Carboxy fluorescein

664. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a fluorescent label.

665. The MHC multimer according to claim 664, wherein the one or more fluorescent label is a simple fluorescent label

666. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group Fluor dyes, Pacific Blue™, Pacific Orange™, Cascade Yellow™

667. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group AlexaFluor@(AF), AF405,

AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750, AF800.

668. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group Quantum Dot based dyes, QDot®

Nanocrystals (Invitrogen, MolecularProbs), Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800.

669. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group DyLight™ Dyes (Pierce) (DL);

DL549, DL649, DL680, DL800.

670. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group Fluorescein (Flu) or any derivate of that, such as FITC

671. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group Cy-Dyes, Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7.

672. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group Fluorescent Proteins, RPE, PerCp, APC, Green fluorescent proteins; GFP and GFP derived mutant proteins; BFP,CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine, mStrawberry, mCherry.

673. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group Tandem dyes, RPE-Cy5, RPE- Cy5.5, RPE-Cy7, RPE-AlexaFluor® tandem conjugates; RPE-Alexa610, RPE-TxRed, APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5, APC-Cy5.5.

674. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group multi fluorochrome assemblies, Multiple fluorochromes attached to a polymer molecule, such as a peptide/protein, Dextrane, polysaccharide, any combination of the fluorescent dyes involving in generation of FRET (Fluorescence resonance energy transfer) based techniques.

675. The MHC multimer according to claim 664, wherein the one or more simple fluorescent label is selected from the group ionophors; ion chelating fluorescent props, props that change wavelength when binding a specific ion, such as

Calcium, props that change intensity when binding to a specific ion, such as Calcium.

676. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is capable of absorption of light

677. The MHC multimer according to claim 676, wherein the one or more labels capable of absorption of light is a chromophore.

678. The MHC multimer according to claim 676, wherein the one or more labels capable of absorption of light is a dye.

679. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is capable of emission of light after excitation

680. The MHC multimer according to claim 679, wherein the one or more labels capable of emission of light is one or more fluorochromes.

681. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the AlexaFluor@(AF) family, which include AF®350,

AF405, AF430, AF488,AF500, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF633, AF635, AF647, AF680, AF700, AF710, AF750 and AF800

682. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the Quantum Dot (Qdot®) based dye family, which include Qdot®525, Qdot®565, Qdot®585, Qdot®605, Qdot®655, Qdot®705, Qdot®800

683. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the DyLight™ Dyes (DL) family, which include DL549,

DL649, DL680, DL800

684. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Small fluorescing dyes, which include FITC, Pacific Blue™, Pacific Orange™, Cascade Yellow™, Marina blue™, DSred,

DSred-2, 7-AAD, TO-Pro-3.

685. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Cy-Dyes, which include Cy2, Cy3, Cy3.5, Cy5, Cy5.5, Cy7

686. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Phycobili Proteins, which include R- Phycoerythrin (RPE), PerCP, Allophycocyanin (APC), B-Phycoerythrin, C- Phycocyanin.

687. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Fluorescent Proteins, which include (E)GFP and GFP ((enhanced) green fluorescent protein) derived mutant proteins; BFP, CFP, YFP, DsRed, T1 , Dimer2, mRFP1 ,MBanana, mOrange, dTomato, tdTomato, mTangerine.

688. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Tandem dyes with RPE, which include

RPE-Cy5, RPE-Cy5.5, RPE-Cy7, RPE-AlexaFIuor® tandem conjugates; RPE- Alexa610, RPE-TxRed.

689. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Tandem dyes with APC, which include

APC-Aleca600, APC-Alexa610, APC-Alexa750, APC-Cy5, APC-Cy5.5.

690. The MHC multimer according to claim 680, wherein the one or more fluorochrome is selected from the family of Calcium dyes, which include Indo-1 -Ca²⁺ lndo-2-Ca²⁺.

691. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is capable of reflection of light

692. The MHC multimer according to claim 691 , wherein the one or more labels capable of reflection of light comprises gold

693. The MHC multimer according to claim 691 , wherein the one or more labels capable of reflection of light comprises plastic

694. The MHC multimer according to claim 691 , wherein the one or more labels capable of reflection of light comprises glass

695. The MHC multimer according to claim 691 , wherein the one or more labels capable of reflection of light comprises polystyrene

696. The MHC multimer according to claim 691 , wherein the one or more labels capable of reflection of light comprises pollen

697. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a chemiluminescent label.

698. The MHC multimer according to claim 697, wherein the chemiluminescent labels is selected from the group luminol, isoluminol, theromatic acridinium ester, imidazole, acridinium salt and oxalate ester.

699. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a bioluminescent label.

700. The MHC multimer according to claim 699, wherein the bioluminescent labels is selected from the group consisting of luciferin, luciferase and aequorin.

701. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a radioactive label.

702. The MHC multimer according to claim 701 , wherein the one or more radioactive labels is a radionuclide.

703. The MHC multimer according to claim 701 , wherein the one or more radioactive labels is an isotope.

704. The MHC multimer according to claim 701 , wherein the one or more radioactive labels comprises α rays.

705. The MHC multimer according to claim 701 , wherein the one or more radioactive labels comprises β rays.

706. The MHC multimer according to claim 701 , wherein the one or more radioactive labels comprises y rays.

707. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is detectable by NMR (nuclear magnetic resonance form paramagnetic molecules)

708. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is an enzyme label.

709. The MHC multimer according to claim 708, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, producing a light signal (chemi-luminescence)

710. The MHC multimer according to claim 708, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, resulting in precipitation of chromophor dyes

71 1. The MHC multimer according to claim 708, wherein the enzyme catalyze a reaction between chemicals in the near environment of the labeling molecules, resulting in precipitates that can be detected by an additional layer of detection molecules

712. The MHC multimer according to claim 708, wherein the enzyme label is selected from the group peroxidases, malate dehydrogenase, staphylococcal nuclease, delta-5-steroid isomerase, yeast alcohol dehydrogenase, alpha- glycerophosphate, dehydrogenase, triose phosphate isomerase, horseradish peroxidase, alkaline phosphatase, asparaginase, glucose oxidase, beta- galactosidase, ribonuclease, urease, catalase, glucose-6-phosphate dehydrogenase, glucoamylase and acetylcholinesterase.

713. The MHC multimer according to claim 708, wherein the enzyme label is horseradish peroxidase.

714. The MHC multimer according to claim 708, wherein the enzyme label is horseradish peroxidase and the substrate is diaminobenzidine (DAB).

715. The MHC multimer according to claim 708, wherein the enzyme label is horseradish peroxidase and the substrate is 3-amino-9-ethyl-carbazole (AEC+).

716. The MHC multimer according to claim 708, wherein the enzyme label is horseradish peroxidase and the substrate is biotinyl tyramide.

717. The MHC multimer according to claim 708, wherein the enzyme label is horseradish peroxidase and the substrate is fluorescein tyramide.

718. The MHC multimer according to claim 708, wherein the enzyme label is alkaline phosphatase.

719. The MHC multimer according to claim 708, wherein the enzyme label is alkaline phosphatase and the substrate is Fast red dye

720. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a ionophore or chelating chemical compound binding to specific ions such as Ca²⁺

721. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a lanthanide.

722. The MHC multimer according to claim 721 , wherein the lanthanide comprises fluorescence.

723. The MHC multimer according to claim 721 , wherein the lanthanide comprises Phosphorescence.

724. The MHC multimer according to claim 721 , wherein the lanthanide is paramagnetic

725. The MHC multimer according to any of claims 618 to 637, wherein the one or more labels is a DNA fluorescing stain

726. The MHC multimer according to claim 725, wherein the DNA fluorescing stain is Propidium iodide

727. The MHC multimer according to claim 725, wherein the DNA fluorescing stain is Hoechst stain

728. The MHC multimer according to claim 725, wherein the DNA fluorescing stain is DAPI

729. The MHC multimer according to claim 725, wherein the DNA fluorescing stain is AMC

730. The MHC multimer according to claim 725, wherein the DNA fluorescing stain is DraQ5™

731. The MHC multimer according to claim 725, wherein the DNA fluorescing stain is Acridine orange

732. The MHC multimer according to claim 361 , wherein the MHC-peptide complex (a-b-P) is attached to the second multimerization domain comprising an avidin or streptavidin via a linkage comprising a biotin moiety.

733. The MHC multimer according to any of claims 582 to 732, wherein the MHC-peptide complex is linked to the second multimerization domain by a second linker moiety.

734. The MHC multimer according to claim 733, wherein the MHC-peptide complex is linked to the second multimerization domain by a covalent linker moiety.

735. The MHC multimer according to claims 733 and 734, wherein the linkage of the second multimerization domain and at least one MHC-peptide complexes is formed by a binding entity X attached to, or being part of, the second multimerization domain, and a binding entity Y attached to, or being part of at least one of the MHC- peptide complexes.

736. The MHC multimer according to claims 733 and 734, wherein the linker moiety linking the second multimerization domain and the MHC-peptide complex comprises the linker moiety XY, wherein the linker moiety XY results from a reaction of the moiety X comprising one or more reactive groups and the moiety Y comprising one or more reactive groups, wherein at least some of said reactive groups are capable of reacting with each other.

737. The MHC multimer according to claim 736, wherein the moiety X comprises a nucleophilic group.

738. The MHC multimer according to claim 737, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -NH-NH₂.

739. The MHC multimer according to claim 736, wherein the moiety Y comprises an electrophilic group.

740. The MHC multimer according to claim 739, wherein the electrophilic group is selected from the group consisting of CHO, COOH and CO.

741. The MHC multimer according to claims 733 and 734, wherein at least one of the reactive groups on one of the moieties X and Y comprises a radical capable of reacting with a reactive group forming part of the other moiety.

742. The MHC multimer according to claims 733 and 734, wherein X and Y comprises reactive groups natively associated with the second multimerization domain and/or the MHC-peptide complexes.

743. The MHC multimer according to claims 733 and 734, wherein X and Y comprises reactive groups not natively associated with the second multimerization domain and/or the MHC-peptide complex.

744. The MHC multimer according to claims 733 and 734, wherein the linker moiety forms a covalent link between the second multimerization domain and at least one of the MHC-peptide complexes.

745. The MHC multimer according to claims 733 and 734, wherein the reactive groups of MHC-peptide complexes include amino acid side chains selected from the group consisting of -NH₂, -OH, -SH, and -NH-

746. The MHC multimer according to claims 733 and 734, wherein the reactive groups of the second multimerization domain include hydroxyls of polysaccharides such as dextrans

747. The MHC multimer according to claims 733 and 734, wherein the reactive groups of the second multimerization domain selected from the group consisting of amino acid side chains comprising -NH₂, -OH, -SH, and -NH- of polypeptides

748. The MHC multimer according to claims 733 and 734, wherein one of the polypeptides of the MHC-peptide complex is linked by a protein fusion to the second multimerization domain

749. The MHC multimer according to claims 733 and 734, wherein one of the polypeptides of the MHC-peptide complex is linked by a protein fusion to the second multimerization domain, wherein an acyl group and an amino group react to form an amide bond

750. The MHC multimer according to claim 363, wherein one of the polypeptides of the MHC-peptide complex is linked by non-native reactive groups to the second multimerization domain.

751. The MHC multimer according to claim 363, wherein the reactive groups include reactive groups that are attached to the second multimerization domain through association of a linker molecule comprising the reactive group.

752. The MHC multimer according to claim 363, wherein the reactive groups include reactive groups that are attached to the MHC-peptide complex through association of a linker molecule comprising the reactive group.

753. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more nucleophilic groups

754. The MHC multimer according to claim 753, wherein the nucleophilic group is selected from the group consisting of -NH₂, -OH, -SH, -CN, -NH-NH₂

755. The MHC multimer according to claim 363, wherein the second multimerization domain is selected from the group consisting of polysaccharides, polypeptides comprising e.g. lysine, serine, and cysteine.

756. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more electrophilic groups.

757. The MHC multimer according to claim 756, wherein the electrophilic group is selected from the group consisting of -COOH, -CHO, -CO, NHS-ester, tosyl- activated ester, and other activated esters, acid-anhydrides.

758. The MHC multimer according to claim 363, wherein the second multimerization domain is selected from the group consisting of polypeptides comprising e.g. glutamate and aspartate, or vinyl sulfone activated dextran.

759. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more radicals.

760. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more conjugated double bonds.

761. The MHC multimer according to claim 363, wherein the second multimerization domain comprises one or more beads, further comprising a linker moiety

762. The MHC multimer according to any of the claims 733 to 761 , wherein the linker is a flexible linker.

763. The MHC multimer according to any of the claims 733 to 761 , wherein the linker is a rigid linker.

764. The MHC multimer according to any of the claims 733 to 761 , wherein the linker is a water-soluble linker.

765. The MHC multimer according to any of the claims 733 to 761 , wherein the linker is a cleavable linker.

766. The MHC multimer according to claim 765, wherein the cleavable linker is selected from linkers depicted in (fig 2).

767. The MHC multimer according to claim 765, wherein the cleavable linker is cleavable at physiological conditions

768. The MHC multimer according to claims 733 and 733, wherein the MHC- peptide complex is linked to the second multimerization domain by a non-covalent linker moiety.

769. The MHC multimer according to claim 768, wherein the non-covalent linkage comprises natural dimerization

770. The MHC multimer according to claim 769, wherein the natural dimerization comprises antigen-antibody pairs.

771. The MHC multimer according to claim 769, wherein the natural dimerization comprises DNA-DNA interactions

772. The MHC multimer according to claim 768, wherein the non-covalent linkage comprises natural interactions

773. The MHC multimer according to claim 772, wherein the natural interaction comprises biotin and streptavidin.

774. The MHC multimer according to claim 604, wherein the bead is coated with streptavidin monomers, which in turn are associated with biotinylated MHC complexes.

775. The MHC multimer according to claim 604, wherein the bead is coated with streptavidin tetramers, which in turn are associated with with 0, 1 , 2, 3, or 4 biotinylated MHC complexes.

776. The MHC multimer according to claim 604, wherein the bead is coated with a polysaccharide, such as a polysaccharide comprising a dextran moiety.

777. The MHC multimer according to claim 772, wherein the natural interaction comprises the interaction of MHC complexes (comprising full-length polypeptide chains, including the transmembrane portion) with the cell membrane of for example dendritic cells.

778. The MHC multimer according to claim 768, wherein the non-covalent linkage comprises artificial interactions.

779. The MHC multimer according to claim 778, wherein the artificial interaction comprises His₆ tag interacting with Ni-NTA.

780. The MHC multimer according to claim 778, wherein the artificial interaction comprises PNA-PNA

781. The MHC multimer according to claim 768, wherein the non-covalent linkage comprises non-specific adsorption.

782. The MHC multimer according to claim 781 , wherein the non-specific adsorption comprises adsorption of proteins onto surfaces.

783. The MHC multimer according to claim 768 wherein the non-covalent linkage comprises the pentamer structure.

784. The MHC multimer according to claim 768, wherein the non-covalent linkage comprises interactions selected from the group streptavidin/biotin, avidin/biotin, antibody/antigen, DNA/DNA, DNA/PNA, DNA/RNA, PNA/PNA, LNA/DNA, leucine zipper e.g. Fos/Jun, IgG dimeric protein, IgM multivalent protein, acid/base coiled-coil helices, chelate/metal ion-bound chelate, streptavidin (SA) and avidin and derivatives thereof, biotin, immunoglobulins, antibodies (monoclonal, polyclonal, and recombinant), antibody fragments and derivatives thereof, leucine zipper domain of AP-1 (jun and fos), hexa-his (metal chelate moiety), hexa-hat GST (glutathione S-transferase) glutathione affinity, Calmodulin-binding peptide (CBP), Strep-tag, Cellulose Binding Domain, Maltose Binding Protein, S-Peptide Tag, Chitin Binding Tag, Immuno-reactive Epitopes, Epitope Tags, E2Tag, HA Epitope Tag, Myc Epitope, FLAG Epitope, AU1 and AU5 Epitopes, GIu-GIu Epitope, KT3 Epitope, IRS Epitope, Btag Epitope, Protein Kinase-C Epitope, VSV Epitope, lectins that mediate binding to a diversity of compounds, including carbohydrates, lipids and proteins, e.g. Con A (Canavalia ensiformis) or WGA (wheat germ agglutinin) and tetranectin or Protein A or G (antibody affinity).

785. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from a virus polypeptide.

786. The monomer or MHC multimer according to claim 785, wherein the virus is selected from the group consisting of Adenovirus (subgropus A-F), BK-virus, CMV (Cytomegalo virus, HHV-5), EBV (Epstein Barr Virus, HHV-4), HBV (Hepatitis B Virus), HCV (Hepatitis C virus), HHV-6a and b (Human Herpes Virus-6a and b), HHV-7, HHV-8, HSV-1 (Herpes simplex virus-1 , HHV-1 ), HSV-2 (HHV-2), JC-virus, SV-40 (Simian virus 40), VZV (Varizella-Zoster-Virus, HHV-3), Parvovirus B19,

Haemophilus influenza, HIV-1 (Human immunodeficiency Virus-1 ), HTLV-1 (Human T-lymphotrophic virus-1 ), HPV (Human Papillomavirus giving rise to clinical manifestions such as Hepatitis, AIDS, Measles, Pox, Chicken pox, Rubella, and Herpes.

787. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from a bacterial polypeptide.

788. The monomer or MHC multimer according to claim 787, wherein the bacterium is selected from the group consisting of Gram positive bacteria, Gram negative bacteria, intracellular bacterium, extracellular bacterium, Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium avium subsp. paratuberculosis Borrelia burgdorferi, other spirochetes, Helicobacter pylori, Streptococcus pneumoniae, Listeria monocytogenes, Histoplasma capsulatum, Bartonella henselae, Bartonella quintana giving rise to clinical manifestations, such as Tuberculosis,

Pneumonia, Stomach ulcers, and Paratuberculosis.

789. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from a fungal polypeptide.

790. The monomer or MHC multimer according to claim 789, wherein the virus is selected from the group consisting of Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, and Pneumocystis carinii giving rise to clinical manifestations, such as skin-, nail-, and mucosal infections, Meningitis, and Sepsis.

791. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from a polypeptide produced by a parasitic organism.

792. The monomer or MHC multimer according to claim 791 , wherein the parasitic organism is selected from the group consisting of Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Schistosoma mansoni, Schistosoma japonicum, Schistosoma haematobium, Trypanosoma cruzi, Trypanosoma rhodesiense, Trypanosoma gambiense, Leishmania donovani, and Leishmania tropica.

793. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from a polypeptide produced by Birch, Hazel, Elm, Ragweed, Wormwood, Grass, Mould, and Dust Mite giving rise to clinical manifestations such as Asthma.

794. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from minor histocompatibility antigens, such as HA-1 , HA-8, USP9Y, SMCY, TPR-protein, HB-1 Y.

795. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from Survivin, Survivin-2B, Livin/ML-IAP, Bcl-2, Mcl-1 , BcI-X(L), Mucin-1 , NY-ESO-1 , Telomerase, CEA, MART- 1 , HER-2/neu, bcr-abl, PSA, PSCA, Tyrosinase, p53, hTRT, Leukocyte Proteinase-3, hTRT, gpl OO, MAGE antigens, GASC, JMJD2C, JARD2 (JMJ), JHDM3a, WT-1 ,CA 9, and protein kinases.

796. The monomer or MHC multimer according to any of claims 1 to 784, wherein the peptide P originates from and/or is isolated from antigens, such as GAD64, Collagen, human cartilage glycoprotein 39, β-amyloid, Aβ42, APP, and Presenilin 1.

797. The monomer or MHC multimer according to any of claims 1 to 796, wherein the peptide P originates from and/or is isolated from

798. A method for generating the MHC multimer according to any of claims 1 to 797, said method comprising the steps of

i) providing one or more peptides P; ii) providing one or more functional MHC proteins, iii) optionally providing one or more multimerization domains, and iv) contacting or reacting the one or more peptides P and the one or more functional

MHC proteins and the one or more multimerization domains simultaneously or sequentialy in any order, thereby obtaining MHC multimers according to the present invention.

799. The method of claim 798, wherein said reaction(s) involves a covalent coupling of the MHC-peptide complex and the multimerization domain(s) comprising nucleophilic substitution by activation of electrophiles, preferably acylation, such as amide formation, pyrazolone formation, isoxazolone formation; alkylation; vinylation; and disulfide formation.

800. The method of claim 798, wherein said reaction(s) involves a covalent coupling of the MHC-peptide complex and the multimerization domain comprising addition to carbon-hetero multiple bonds, preferably by alkene formation by reaction of phosphonates with aldehydes or ketones; or arylation; alkylation of arenes/hetarenes by reaction with alkyl boronates or enolethers.

801. The method of claim 798, wherein said reaction(s) involves a covalent coupling of the MHC-peptide complex and the multimerization domain comprising nucleophilic substitution using activation of nucleophiles, preferably by condensations; alkylation of aliphatic halides or tosylates with enolethers or enamines.

802. The method of claim 798, wherein said reaction involves a covalent coupling of the MHC-peptide complex and the multimerization domain comprises cycloadditions.

803. The method of claim 798, wherein the one or more MHC peptide complexes comprises MHC class I heavy chain and/or beta2-Microglobulin.

804. The method of claim 803, wherein the MHC class I heavy chain and/or beta2-Microglobulin is derived from natural sources.

805. The method of claim 803, wherein the MHC class I heavy chain and/or beta2-Microglobulin is generated by recombinant means.

806. The method of claim 805, wherein the recombinant means comprises in vitro translation of mRNA obtained from expressing cells.

807. The method of claim 805, wherein the recombinant means comprises use of beta2-microglubulin and/or MHC class I Heavy chain transfected cells.

808. The method of claim 807, wherein the use of beta-2-microglubulin and/or

MHC class I Heavy chain transfected cells comprises use of genetic material isolated from natural origin such as cells, one or more tissues and/or one or more organisms.

809. The method of claim 807, wherein the use of beta-2-microglubulin and/or MHC class I Heavy chain transfected cells comprises use of one or more synthetic genes identical to natural DNA sequence and/or modified.

810. The method of claim 807, wherein the beta-2-microglubulin is full length.

81 1 . The method of claim 807, wherein the beta-2-microglubulin is a fragment.

812. The method of claim 803, wherein the MHC class I Heavy chain is full length.

813. The method of claim 803, wherein the MHC class I Heavy chain is a fragment.

814. The method of claim 813, wherein the MHC class I Heavy chain fragment comprises and/or consists of the complete chain minus the intramembrane domain.

815. The method of claim 813, wherein the MHC class I Heavy chain fragment comprises and/or consists of the alphal and alpha 2 class 1 heavy chain domains.

816. The method of claims 803 and 815, wherein the beta2-microglubulin and/or MHC class I Heavy chain fragment comprises one or more added designer domain(s) and/or sequences(s).

817. The method of claim 803, wherein the MHC class I heavy chain and/or beta2-Microglobulin is generated by chemical synthesis.

818. The method of claim 817, wherein the chemical synthesis comprises solid phase synthesis.

819. The method of claim 798, wherein the one or more MHC peptide complexes comprises MHC class Il alpha and/or beta chain.

820. The method of claim 819, wherein the MHC class Il alpha and/or beta chain is derived from natural sources.

821. The method of claim 819, wherein the MHC class Il alpha and/or beta chain is generated by recombinant means.

822. The method of claim 821 , wherein the recombinant means comprises in vitro translation of mRNA obtained from expressing cells.

823. The method of claim 821 , wherein the recombinant means comprises use of MHC class Il alpha and/or beta chain transfected cells.

824. The method of claim 823, wherein the use of MHC class Il alpha and/or beta chain transfected cells comprises use of genetic material isolated from natural origin such as cells, one or more tissues and/or one or more organisms.

825. The method of claim 823, wherein the use of MHC class Il alpha and/or beta chain transfected cells comprises use of one or more synthetic genes identical to natural DNA sequence and/or modified.

826. The method of claim 823, wherein the MHC class Il alpha and/or beta chain is full length.

827. The method of claim 823, wherein the MHC class Il alpha and/or beta chain is a fragment.

828. The method of claim 827, wherein the MHC class Il alpha and/or beta chain fragment comprises and/or consists of the MHC class 2 α- and β-chain fragments consisting of, the complete α- and β-chains minus the intramembrane domains of either or both chains.

829. The method of claim 827, wherein the MHC class Il alpha and/or beta chain fragment comprises and/or consists of α- and β-chains consisting of only the extracellular domains of either or both, i.e α1 plus α2 and β1 plus β2 domains, respectively.

830. The method of claim 827, wherein the MHC class Il alpha and/or beta chain fragment comprises and/or consists of the MHC class Il molecule fragments consisting of domains starting from the amino terminal in consecutive order, MHC class 2 β1 plus MHC class 2 α1 plus MHC class 1 α3 domains or in alternative order, MHC class 2 α1 plus MHC class 2 β1 plus MHC class 1 α3 domains.

831. The method of claim 819 to 830, wherein the MHC class Il alpha and/or beta chain fragment comprises one or more modified or added designer domain(s) and/or sequence(s).

832. The method of claim 798, wherein the one or more MHC peptide complexes are modified.

833. The method of claim 832, wherein the one or more MHC peptide complexes that are modified are one or more MHC class I complexes and/or one or more MHC class Il complexes.

834. The method of claim 833, wherein the one or more MHC class I complexes and/or one or more MHC class Il complexes are modified by mutagenesis.

835. The method of claim 834, wherein the mutagenesis comprises one or more substitutions of one or more natural and/or non-natural amino acids.

836. The method of claim 834, wherein the mutagenesis comprises one or more deletions of one or more natural and/or non-natural amino acids.

837. The method of claim 834, wherein the mutagenesis comprises one or more insertions of one or more natural and/or non-natural amino acids.

838. The method of claim 834, wherein the mutagenesis comprises one or more mutations in the α3 subunit of MHC I heavy chain.

839. The method of claim 834, wherein the mutagenesis comprises one or more mutations in areas of the β2-domain of MHC Il molecules responsible for binding CD4 molecules.

840. The method of claim 833, wherein the one or more MHC class I complexes and/or one or more MHC class Il complexes are modified by one or more chemical modification(s).

841. The method of claim 840, wherein the one or more chemical modification(s) comprises one or more chemical modification(s) of the peptide in the peptide-binding cleft such as attachment of a dinitrophenyl group.

842. The method of claim 840, wherein the one or more chemical modification(s) comprises one or more chemical modification(s) of MHC I or MHC Il fusion proteins.

843. The method of claim 840, wherein the one or more chemical modification(s) comprises one or more chemical modification(s) of MHC complexes fused with genes encoding an amino acid sequence (biotinylation sequence) capable of being biotinylated with a Bir A enzyme.

844. The method of claim 843, wherein the biotinylation sequence is fused with the COOH-terminal of β2m or the heavy chain of MHC I molecules or the COOH- terminal of either the α-chain or β-chain of MHC II.

845. The method of claim 843, wherein the biotinylation sequence is fused with the NH₂-terminal of β2m or the heavy chain of MHC I molecules or the NH₂-terminal of either the α-chain or β-chain of MHC II.

846. The method of claim 840, wherein the one or more chemical modification(s) comprises one or more chemical modification(s) of MHC complexes fused with genes encoding an amino acid sequence capable of being chemically modified.

847. The method of claim 846, wherein the one or more genes encoding an amino acid sequence capable of being chemically modified are fused with the COOH-terminal of β2m or the heavy chain of MHC I molecules or the COOH-terminal of either the α-chain or β-chain of MHC II.

848. The method of claim 846, wherein the one or more genes encoding an amino acid sequence capable of being chemically modified are fused with the NH₂- terminal of β2m or the heavy chain of MHC I molecules or the NH₂-terminal of either the α-chain or β-chain of MHC II.

849. The method of claim 798, wherein the one or more MHC peptide complexes and/or empty MHC complexes are stabilized.

850. The method of claim 849, wherein the one or more MHC peptide complexes and/or empty MHC complexes that are stabilized are one or more MHC class I complexes.

851. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises generation of covalent protein-fusions.

852. The method of claim 851 , wherein the generation of covalent protein- fusions comprises introduction of one or more linkers between the individual components of the MHC I complex.

853. The method of claim 851 , wherein the generation of covalent protein- fusions comprises introduction of one or more linkers resulting in generation of a complex comprising a heavy chain fused with β2m through a linker and a soluble peptide.

854. The method of claim 851 , wherein the generation of covalent protein- fusions comprises introduction of one or more linkers resulting in generation of a complex comprising a heavy chain fused to β2m through a linker.

855. The method of claim 851 , wherein the generation of covalent protein- fusions comprises introduction of one or more linkers resulting in generation of a complex comprising a heavy chain /β2m dimer covalently linked to a peptide through a linker to either heavy chain or β2m, and where there can or can not be a linker between the heavy chain and β2m.

856. The method of claim 851 , wherein the generation of covalent protein- fusions comprises introduction of one or more linkers resulting in generation of a complex comprising a heavy chain fused to a peptide through a linker.

857. The method of claim 851 , wherein the generation of covalent protein- fusions comprises introduction of one or more linkers resulting in generation of a complex comprising the α1 and α2 subunits of the heavy chain fused to a peptide through a linker.

858. The method of claim 852-857, wherein the heavy chain, beta2- microglubulin and/or the peptide is truncated.

859. The method of claim 852-857, wherein the linker is a flexible linker.

860. The method of claim 859 wherein the flexible linker is made of glycine and/or serine.

861. The method of claim 859, wherein the flexible linker is between 5-20 residues long such as 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18 or 19 residues long.

862. The method of claim 852-857, wherein the linker is rigid with a defined structure.

863. The method of claim 962, wherein the rigid linker is made of amino acids like glutamate, alanine, lysine, and leucine creating e.g. a more rigid structure.

864. The method of claim 863, wherein the rigid linker is between 5-20 residues long such as 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18 or 19 residues long.

865. The method of claim 863, wherein the one or more stabilized MHC peptide complexes and/or empty MHC complexes comprises heavy chain-β2m fusion proteins.

866. The method of claim 865, wherein the heavy chain-β2m fusion proteins is constructed so that the COOH terminus of β2m is covalently linked to the NH₂ terminus of the heavy chain.

867. The method of claim 865, wherein the heavy chain-β2m fusion proteins is constructed so that the NH₂ terminus of β2m is linked to the COOH terminus of the heavy chain.

868. The method of claim 849, wherein the one or more stabilized MHC peptide complexes and/or empty MHC complexes comprises one or more peptide- β2m fusion proteins.

869. The method of claim 868, wherein one or more peptide-β2m fusion proteins are constructed so that the COOH terminus of the peptide is linked to the NH₂ terminus of β2m.

870. The method of claim 868, wherein one or more peptide-β2m fusion proteins are constructed so that the peptide is linked to the COOH terminal of β2m via its NH₂ terminus.

871. The method of claim 849, wherein the one or more stabilized MHC peptide complexes and/or empty MHC complexes comprises one or more heavy chain-peptide fusion proteins.

872. The method of claim 871 , wherein the one or more heavy chain-peptide fusion proteins are constructed so that the NH₂ terminus of the heavy chain is fused to the COOH terminus of the peptide.

873. The method of claim 871 , wherein the one or more heavy chain-peptide fusion proteins are constructed so that the COOH terminus of the heavy chain is fused to the NH₂ terminus of the peptide.

874. The method of claim 849, wherein the one or more stabilized MHC peptide complexes and/or empty MHC complexes comprises one or more heavy chain-β2m-peptide fusion proteins.

875. The method of claim 874, wherein the one or more heavy chain-β2m- peptide fusion proteins is constructed so that the NH₂ terminus of the heavy chain is fused to the COOH terminus of β2m and the NH₂ terminus of β2m is fused to the COOH terminus of the peptide.

876. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises non-covalent stabilization by binding to one or more unnatural component.

877. The method of claim 876, wherein the one or more unnatural component bind to both the heavy chain and the β2m and thereby promote the assembly of the complex, and/or stabilize the formed complex.

878. The method of claim 876, wherein the one or more unnatural component bind to either β2m or heavy chain, and in this way stabilize the polypeptide in its correct conformation, and in this way increase the affinity of the heavy chain for β2m and/or peptide, or increase the affinity of β2m for peptide.

879. The method of claim 876, wherein the one or more unnatural component comprises one or more antibodies.

880. The method of claim 879, wherein the one or more antibodies can be selected from the group consisting of truncated or full-length antibodies of isotype IgG, IgM, IgA, IgE, Fab, scFv or bi-Fab fragments or diabodies.

881. The method of claim 879, wherein the one or more antibodies comprises one or more antibodies binding the MHC I molecule by interaction with the heavy chain as well as β2m.

882. The method of claim 879, wherein the one or more antibodies comprises one or more bispecific antibodies that binds with one arm to the heavy chain and the other arm to the β2m of the MHC complex.

883. The method of claim 879, wherein the one or more antibodies comprises one or more monospecific antibodies which bind at the interface between heavy chain and β2m.

884. The method of claim 879, wherein the one or more antibodies comprises one or more antibodies that bind the correctly folded heavy chain with higher affinity than non-correctly folded heavy chain.

885. The method of claim 879, wherein the one or more antibodies comprises an antibody like the one produced by the clone W6/32 (M0736 from Dako, Denmark) that recognizes a conformational epitope on intact human and some monkey MHC complexes containing β2m, heavy chain and peptide.

886. The method of claim 876, wherein the one or more unnatural component comprises one or more peptides.

887. The method of claim 876, wherein the one or more unnatural component comprises one or more aptamers.

888. The method of claim 876, wherein the one or more unnatural component comprises one or more molecule with the ability to bind to the surface of the MHC complex.

889. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises generation of modified proteins or protein components.

890. The method of claim 889, wherein the generation of modified proteins or protein components results in an increase of the affinity of the peptide P for the MHC complex.

891. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by mutation/substitution of amino acids at relevant positions in the peptide.

892. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by chemical modifications of amino acids at relevant positions in the peptide.

893. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by introduction of non-natural amino acids at relevant positions in the peptide.

894. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by mutations, chemical modifications, insertion of natural or non-natural amino acids or deletions in the peptide binding cleft, i.e. in the binding pockets that accommodate peptide side chains responsible for anchoring the peptide to the peptide binding cleft.

895. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by introduction of reactive groups into the antigenic peptide; before, during or upon binding of the peptide.

896. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by mutations/substitutions, chemical modifications, insertion of natural or non-natural amino acids or deletions in the heavy chain and/or β2m at positions outside the peptide-binding cleft.

897. The method of claim 890, wherein the increase of the affinity of the peptide P for the MHC complex is caused by removal of cysteine residues in the heavy chain by mutation, chemical modification, amino acid exchange or deletion.

898. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises stabilization with soluble additives.

899. The method of claim 898, wherein the stabilization with soluble additives comprises addition of one or more additives selected from the group consisting of salts, detergents organic solvent and polymers.

900. The method of claim 898, wherein the stabilization with soluble additives comprises addition one or more additives that increase surface tension of the MHC molecule without binding the molecule.

901. The method of claim 900, wherein the one or more additives that increase surface tension of the MHC molecule without binding the molecule can be selected from the group consisting of sucrose, mannose, glycine, betaine, alanine, glutamine, glutamic acid and ammoniumsulfate.

902. The method of claim 898, wherein the stabilization with soluble additives comprises addition one or more additives that increase surface tension of the MHC molecule.

903. The method of claim 902, wherein the one or more additives that increase surface tension of the MHC molecule can be selected from the group consisting of glycerol, mannitol and sorbitol.

904. The method of claim 898, wherein the stabilization with soluble additives comprises addition one or more additives that increase surface tension of the MHC molecule and simultaneously interact with charged groups in the protein.

905. The method of claim 904, wherein the one or more additives that increase surface tension of the MHC molecule and simultaneously interact with charged groups in the protein can be selected from the group consisting of MgSO₄,

NaCI, polyethylenglycol, 2-methyl-2,4-pentandiol and guanidiniumsulfate.

906. The method of claim 898, wherein the stabilization with soluble additives comprises addition of molar excess of peptide.

907. The method of claim 898, wherein the stabilization with soluble additives comprises addition of excess of β2m.

908. The method of claim 898, wherein the stabilization with soluble additives comprises addition BSA, fetal and bovine calf serum and/or individual protein components in serum with a protein stabilizing effect.

909. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises stabilization with soluble additives, said additives are added during the refolding process.

910. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises stabilization with soluble additives, said additives are added to the soluble monomer.

91 1. The method of claim 850, wherein the stabilization of one or more MHC class I complexes comprises stabilization with soluble additives, said additives are added to a solutions containing MHC I bound to a carrier.

912. The method of claim 849, wherein the one or more MHC peptide complexes and/or empty MHC complexes that are stabilized are one or more MHC class Il complexes.

913. The method of claim 912, wherein the stabilization of one or more MHC class I complexes and/or one or more MHC class Il complexes comprises generation of covalent protein-fusions.

914. The method of claim 913, wherein the generation of covalent protein- fusions comprises stabilization of MHC Il complexes by introduction of one or more linkers between the individual components of the MHC Il complex.

915. The method of claim 913, wherein the generation of covalent protein- fusions comprises stabilization of MHC Il complexes by construction of a α/β dimer with a linker between α-chain and β-chain.

916. The method of claim 913, wherein the generation of covalent protein- fusions comprises stabilization of MHC Il complexes by construction of a α/β dimer covalently linked to the peptide via a linker to either the α-chain or β-chain.

917. The method of claim 913, wherein the generation of covalent protein- fusions comprises stabilization of MHC Il complexes by construction of a α/β dimer, covalently linked by a linker between the α-chain and β-chain, and where the dimer is covalently linked to the peptide.

918. The method of claim 913, wherein the generation of covalent protein- fusions comprises stabilization of MHC Il complexes by construction of a α/β dimer with a linker between α-chain and β-chain, where the dimer is combined with a peptide covalently linked to either α-chain or β-chain.

919. The method of claim 913, wherein the generation of covalent protein- fusions comprises one or more linkers.

920. The method of claim 919, wherein the one or more linkers comprises one or more flexible linker(s).

921. The method of claim 920, wherein the one or more flexible linker(s) comprises glycine and/or serine.

922. The method of claim 920, wherein the one or more flexible linker(s) are between 5-20 residues long such as 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18 or 19 residues long.

923. The method of claim 919, wherein the one or more linker(s) comprises one or more rigid linker with a more defined structure.

924. The method of claim 923, wherein the one or more rigid linker(s) comprises glutamate, alanine, lysine, and/or leucine.

925. The method of claim 919, wherein the one or more linker(s) result in that the one or more peptide is linked to the NH₂- or COOH-terminus of either α-chain or β-chain.

926. The method of claim 919, wherein the one or more linker(s) result in that the one or more peptides are linked to the NH₂-terminus of the β-chain via their

COOH-terminus.

927. The method of claim 919, wherein the one or more linker(s) result in that the α-chain is linked to the β-chain via the COOH-terminus of the β-chain to the NH₂- terminus of the α-chain or from the COOH-terminus of the α-chain to the NH₂- terminus of the β-chain.

928. The method of claim 919, wherein the one or more linker result in that formation of a three-molecule fusion protein consisting of α-chain, β-chain and peptide, wherein some linker(s) connect the COOH-terminus of the β-chain with the NH₂-terminus of the α-chain and other linker(s) connect the COOH-terminal of the peptide with the NH₂-terminal of the β-chain.

929. The method of claim 919, wherein the one or more linker result in that formation of a three-molecule fusion protein consisting of α-chain, β-chain and peptide, wherein one linker joins the COOH-terminus of the α-chain with the NH₂- terminus of the β-chain and a second linker joins the NH₂-terminus of the peptide with the COOH-terminus of the β-chain.

930. The method of claim 927, wherein the stabilization of one or more MHC class I complexes and/or one or more MHC class Il complexes comprises non- covalent stabilization by binding ligand.

931. The method of claim 930, wherein the non-covalent stabilization by binding ligand results in bridging of α- and β-chain.

932. The method of claim 930, wherein the non-covalent stabilization by binding ligand results in binding to either of the α- or β-chains, and in this way stabilize the conformation of α or β, that binds β or α, respectively, and/or that binds the peptide.

933. The method of claim 930, wherein the non-covalent stabilization by binding ligand comprises one or more compounds selected from the group consisting of antibodies, peptides, aptamers or any other molecules with the ability to bind proteins.

934. The method of claim 933, wherein the one or more antibodies comprises one or more antibodies which bind the MHC complex distal to the interaction site with TCR, i.e. distal to the peptide-binding cleft.

935. The method of claim 933, wherein the one or more antibodies comprises one or more antibodies selected from the group consisting of truncated or full length antibody of any isotype IgG, IgM, IgA or IgE, a bi-Fab fragment or a diabody.

936. The method of claim 933, wherein the one or more antibodies comprises one or more bispecific antibodies e.g. with one arm binding to the α-chain and the other arm binding to the β-chain.

937. The method of claim 933, wherein the one or more antibodies comprises one or more monospecific antibodies e.g. directed to a sequence fused to the α-chain as well as to the β-chain.

938. The method of claim 933, wherein the one or more antibodies comprises one or more monospecific antibodies which are capable of binding to a surface of the complex that involves both the α- and β-chain, e.g. both the α2- and β2- domain or both the α1 - and β1 - domain.

939. The method of claim 933, wherein the one or more antibodies comprises one or more antibodies capable of binding more central in the MHC Il molecule, but still interacting with both α- and β-chain.

940. The method of claim 933, wherein the one or more antibodies comprises one or more antibodies which are capable of binding a conformational epitope.

941 . The method of claim 912, wherein the stabilization of one or more MHC class I complexes and/or one or more MHC class Il complexes comprises non- covalent stabilization by induced multimerization.

942. The method of claim 941 , wherein the non-covalent stabilization by induced multimerization comprises anchoring of the α- and/or β-chains in the cell membrane.

943. The method of claim 941 , wherein the non-covalent stabilization by induced multimerization comprises anchoring the α/β-dimer by attachment of the

MHC Il chains to the Fc regions of an antibody.

944. The method of claim 941 , wherein the non-covalent stabilization by induced multimerization comprises anchoring of one or more MHC Il monomers and/or dimers into one or more artificial membrane spheres like liposomes or lipospheres.

945. The method of claim 941 , wherein the non-covalent stabilization by induced multimerization comprises the steps of biotinylation of α- as well as β-chain, followed by the binding of biotinylated α-chain and β-chain to streptavidin, to form an association between α-chain and β-chain.

946. The method of claim 912, wherein the stabilization of one or more MHC class I complexes and/or one or more MHC class Il complexes comprises generation of modified proteins or protein components.

947. The method of claim 946, wherein the generation of modified proteins or protein components comprises one or more covalent modifications of the protein.

948. The method of claim 946, wherein the generation of modified proteins or protein components comprises exchange of the natural amino acids with other natural or non-natural amino acids at relevant positions in the peptide or by chemical modifications of amino acids at relevant positions in the peptide.

949. The method of claim 9346, wherein the generation of modified proteins or protein components comprises mutations, chemical modifications, insertion of natural or non-natural amino acids or deletions in the peptide-binding cleft.

950. The method of claim 946, wherein the generation of modified proteins or protein components comprises mutations, chemical modifications, insertion of natural or non-natural amino acids or deletions in α- and/or β- chain at positions outside the peptide-binding cleft.

951. The method of claim 946, wherein the generation of modified proteins or protein components comprises replacement of the hydrophobic transmembrane regions of α-chain and β-chain with leucine zipper dimerisation domains (e.g. Fos- Jun leucine zipper; acid-base coiled-coil structure) to promote assembly of α-chain and β-chain.

952. The method of claim 946, wherein the generation of modified proteins or protein components comprises introduction of one or more cysteine residues by amino acid exchange at the COOH-terminal of both α-chain and β-chain, to create disulfide bridges between the two chains upon assembly of the MHC complex.

953. The method of claim 946, wherein the generation of modified proteins or protein components comprises removal of cysteine residues in either of the chains by mutation, chemical modification, amino acid exchange or deletion.

954. The method of claim 946, wherein the generation of modified proteins or protein components comprises stabilization of MHC Il complexes by stabilized by chemically linking together the subunits and the peptide.

955. The method of claim 912, wherein the one or more MHC class I complexes and/or one or more MHC class Il complexes comprises stabilization with one or more soluble additives.

956. The method of claim 955, wherein the one or more soluble additives can be selected from the group consisting of salts, detergents, organic solvent, polymers and any other soluble additives can be added to increase the stability of MHC complexes.

957. The method of claim 955, wherein the one or more soluble additives that can increase the surface tension of the MHC complex.

958. The method of claim 957, wherein the one or more soluble additives that can increase the surface tension of the MHC complex can be selected from the group consisting of sucrose, mannose, glycine, betaine, alanine, glutamine, glutamic acid and ammonium sulphate, glycerol, mannitol, sorbitol, MgSO₄, NaCI, polyethylenglycol, 2-methyl-2,4-pentanediol and guanidiniumsulphate.

959. The method of claim 957, wherein the one or more soluble additives comprises excess of peptide.

960. The method of claim 957, wherein the one or more soluble additives comprises excess of β2m.

961. The method of claim 957, wherein the one or more soluble additives comprises BSA, fetal and bovine calf serum, and other protein components in serum with a protein stabilizing effect.

962. The method of claim 849, wherein the one or more MHC peptide complexes and/or empty MHC complexes are stabilized by chemical modification.

963. The method of claim 962, wherein the chemical modification results in cross-linking of MHC I and/or MHC Il complexes.

964. The method of claim 962, wherein the chemical modification comprises nucleophilic substitution by activation of electrophiles.

965. The method of claim 962, wherein the chemical modification comprises acylation.

966. The method of claim 962, wherein the chemical modification comprises amide formation.

967. The method of claim 962, wherein the chemical modification comprises pyrazolone formation.

968. The method of claim 962, wherein the chemical modification comprises isoxazolone formation.

969. The method of claim 962, wherein the chemical modification comprises alkylation.

970. The method of claim 962, wherein the chemical modification comprises vinylation. T¹JO

971. The method of claim 962, wherein the chemical modification comprises disulfide formation.

972. The method of claim 962, wherein the chemical modification comprises addition to carbon-hetero multiple bonds.

973. The method of claim 962, wherein the chemical modification comprises alkene formation by reaction of phosphonates with aldehydes or ketones.

974. The method of claim 962, wherein the chemical modification comprises arylation.

975. The method of claim 962, wherein the chemical modification comprises alkylation of arenes/hetarenes by reaction with alkyl boronates or enolethers.

976. The method of claim 962, wherein the chemical modification comprises nucleophilic substitution using activation of nucleophiles.

977. The method of claim 962, wherein the chemical modification comprises condensations.

978. The method of claim 962, wherein the chemical modification comprises alkylation of aliphatic halides or tosylates with enolethers or enamines.

979. The method of claim 962, wherein the chemical modification comprises cycloadditions.

980. The method of claim 962, wherein the chemical modification comprises use of one or more reagents selected from the group consisting of activated carboxylic acids, NHS-ester, tetra and pentafluoro phenolic esters, anhydrides, acid chlorides and fluorides, sulphonyl chlorides, iso-Cyanates, isothiocyanates, aldehydes, formaldehyde, and glutardialdehyde.

981. The method of claim 962, wherein the chemical modification comprises modification of one or more cysteine(s).

982. The method of claim 962, wherein the chemical modification comprises modification of one or more cysteine(s) by maleimides, vinyl sulphones and/or halides.

983. The method of claim 962, wherein the chemical modification comprises modification of one or more carboxylic acids at the C-terminal of both chains and/or peptide, as well as on the side chains of glutamic and aspartic acid, by introduction of one or more cross-links e.g. by carbodiimides treatment.

984. The method of claim 962, wherein the chemical modification comprises use of two or more reagents i.e. GMBS can be used to introduce maleimides on the α-chain, and iminothiolane can be used to introduce thiols on the β-chain; the malemide and thiol can then form a thioether link between the two chains.

985. The method of claim 962, wherein the chemical modification comprises that the folded MHC-complex is reacted with dextrans possessing a large number (up to many hundreds) of vinyl sulphones; said vinyl sulphones react with lysine residues on both the α and β chains as well as with lysine residues on the peptide protruding from the binding site, effectively cross linking the entire MHC-complex.

986. The method of claim 962, wherein the chemical modification comprises that one or more lysine residues are inserted into the α-chain, juxtaposed with glutamic acids in the β-chain, where after the introduced amino groups and carboxylic acids are reacted by addition of carbodiimide.

987. The method of claim 962, wherein the chemical modification comprises that a dextran multimerization domain is cross-linked with appropriately modified MHC-complexes; i.e. one or both chains of the MHC complex can be enriched with lysine residues, increasing reactivity towards the vinylsulphone dextran.

988. The method of claim 962, wherein the chemical modification comprises use of extended and flexible cross-linkers.

989. The method of claim 783, wherein the one or more peptides P are selected either from the group consisting of 8-, 9-, 10,- and 1 1 -mer peptides, or from the group consisting of 13-, 14-, 15-, 16-, 17- and 18-mer peptides; corresponding to peptides of MHC classes I and II, respectively.

990. The method of claim 989 comprising the further step of confirming the antigenicity of a peptide P and/or determining the sequence of a peptide P, said method comprising the steps of

selecting one or more MHC alleles; and

obtaining and optionally identifying one or more peptides P of a predetermined length derived from a specific protein, and

assaying the association formed between a) said one or more peptides P, when forming part of a functional MHC protein in a MHC monomer or MHC multimer, and b) a T-cell receptor representative of the selected MHC allelle, and

optionally modifying said one or more peptides P,

thereby confirming or generating one or more optionally modified peptides P of general utility in diagnosing or treating a disease in an individual.

991. The method of claim 990, wherein identification of the one or more peptides P derived from a specific protein for a given MHC allele comprises computational analysis.

992. The method of claim 991 , wherein the computational analysis comprises a prediction of theoretical binding affinity of the peptide to the MHC molecules using www.syfpeithi.de.

993. The method of claim 991 , wherein the computational analysis comprises prediction of theoretical binding affinity of the peptide to the MHC molecules using http://www-bimas.cit.nih.gov/molbio/hla_bind/.

994. The method of claim 991 , wherein the computational analysis comprises prediction of theoretical binding affinity of the peptide to the MHC molecules using www.cbs. dtu.dk/services/NetMHC/.

995. The method of claim 991 , wherein the computational analysis comprises prediction of theoretical binding affinity of the peptide to the MHC molecules using www.cbs.dtu.dk/services/NetMHCII/.

996. The method of claim 991 , wherein the computational analysis comprises translation of the DNA sequence (genomic DNA) in all three reading frames in both directions leading to a total of six amino acid sequences for a given genome; said amino acid sequences are used to predict the MHC molecule peptides P.

997. The method of claim 991 , wherein the computational analysis comprises translation of one or more cDNA sequences into the amino acid sequences; said amino acid sequences are used to predict the MHC molecule peptides P.

998. The method of claim 991 , wherein the computational analysis comprises prediction of MHC molecule peptides P from one or more known amino acid sequences.

999. The method of claim 990, wherein the modification of the one or more antigenic peptides comprises the use of one or more peptides homologous to the predicted peptide sequences; said peptides homologous having an amino acid sequence identity greater than e.g. more than 90%, more than 80% or more than

70%.

1000. The method of claim 999, wherein the one or more homologues MHC peptide sequences arise from the existence of multiple strongly homologous alleles.

1001 . The method of claim 999, wherein the one or more homologues MHC peptide sequences arise from one or more insertions.

1002. The method of claim 999, wherein the one or more homologues MHC peptide sequences arise from one or more deletions.

1003. The method of claim 999, wherein the one or more homologues MHC peptide sequences arise from one or more inversions.

1004. The method of claim 999, wherein the one or more homologues MHC peptide sequences arise from one or more substitutions.

1005. The method of claim 990, wherein the modification of the one or more peptides P comprises the use of one or more peptides with one or more un-common amino acids such as selenocysteine and/or pyrrolysine.

1006. The method of claim 990, wherein the modification of the one or more peptides P comprises the use of one or more peptides with one or more artificial amino acids such as the isomeric D-form.

1007. The method of claim 990, wherein the modification of the one or more peptides P comprises the use of one or more peptides with one or more chemically modified amino acids.

1008. The method of claim 990, wherein the modification of the one or more peptides P comprises the use of one or more split- or combinatorial epitope origin i.e. formed by linkage of peptide fragments derived from two different peptide fragments and/or proteins.

1009. The method of claim 990, wherein the modification of the one or more peptides P comprises one or more modifications at any position of the one or more peptides e.g. at position 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17 and/or 18.

1010. The method of claim 990, wherein the modification of the one or more peptides P comprises one or more post translational modifications.

101 1 . The method of claim 1010, wherein the one or more post translational modifications comprises acylation. *

1012. The method of claim 1010, wherein the one or more post translational modifications comprises alkylation.

1013. The method of claim 1010, wherein the one or more post translational modifications comprises methylation.

1014. The method of claim 1010, wherein the one or more post translational modifications comprises demethylation.

1015. The method of claim 1010, wherein the one or more post translational modifications comprises amidation at C-terminus.

1016. The method of claim 1010, wherein the one or more post translational modifications comprises biotinylation.

1017. The method of claim 1010, wherein the one or more post translational modifications comprises formylation.

1018. The method of claim 1010, wherein the one or more post translational modifications comprises gamma-carboxylation.

1019. The method of claim 1010, wherein the one or more post translational modifications comprises glutamylation.

1020. The method of claim 1010, wherein the one or more post translational modifications comprises glycosylation.

1021. The method of claim 1010, wherein the one or more post translational modifications comprises glycation.

1022. The method of claim 1010, wherein the one or more post translational modifications comprises glycylation.

1023. The method of claim 1010, wherein the one or more post translational modifications comprises covalently attachment of one or more heme moieties.

1024. The method of claim 1010, wherein the one or more post translational modifications comprises hydroxylation.

1025. The method of claim 1010, wherein the one or more post translational modifications comprises iodination.

1026. The method of claim 1010, wherein the one or more post translational modifications comprises isoprenylation.

1027. The method of claim 1010, wherein the one or more post translational modifications comprises lipoylation.

1028. The method of claim 1010, wherein the one or more post translational modifications comprises prenylation.

1029. The method of claim 1010, wherein the one or more post translational modifications comprises GPI anchor formation.

1030. The method of claim 1010, wherein the one or more post translational modifications comprises myristoylation.

1031. The method of claim 1010, wherein the one or more post translational modifications comprises farnesylation.

1032. The method of claim 1010, wherein the one or more post translational modifications comprises geranylation.

1033. The method of claim 1010, wherein the one or more post translational modifications comprises covalently attachment of nucleotides or derivatives thereof.

1034. The method of claim 1010, wherein the one or more post translational modifications comprises ADP-ribosylation.

1035. The method of claim 1010, wherein the one or more post translational modifications comprises flavin attachment.

1036. The method of claim 1010, wherein the one or more post translational modifications comprises oxidation.

1037. The method of claim 1010, wherein the one or more post translational modifications comprises pegylation.

1038. The method of claim 1010, wherein the one or more post translational modifications comprises addition of poly-ethylen-glycol groups to a protein.

1039. The method of claim 1010, wherein the one or more post translational modifications comprises modification of one or more reactive amino acids include lysine, cysteine, histidine, arginine, aspartic acid, glutamic acid, serine, threonine, tyrosine.

1040. The method of claim 1010, wherein the one or more post translational modifications comprises covalently attachment of phosphatidylinositol.

1041 . The method of claim 1010, wherein the one or more post translational modifications comprises phosphopantetheinylation.

1042. The method of claim 1010, wherein the one or more post translational modifications comprises phosphorylation.

1043. The method of claim 1010, wherein the one or more post translational modifications comprises pyroglutamate formation.

1044. The method of claim 1010, wherein the one or more post translational modifications comprises racemization of proline by prolyl isomerase.

1045. The method of claim 1010, wherein the one or more post translational modifications comprises tRNA-mediated addition of amino acids such as arginylation.

1046. The method of claim 1010, wherein the one or more post translational modifications comprises sulfation.

1047. The method of claim 1010, wherein the one or more post translational modifications comprises Selenoylation (co-translational incorporation of selenium in selenoproteins).

1048. The method of claim 1010, wherein the one or more post translational modifications comprises ISGylation.

1049. The method of claim 1010, wherein the one or more post translational modifications comprises SUMOylation.

1050. The method of claim 1010, wherein the one or more post translational modifications comprises ubiquitination.

1051 . The method of claim 1010, wherein the one or more post translational modifications comprises citrullination, or deimination the conversion of arginine to citrulline.

1052. The method of claim 1010, wherein the one or more post translational modifications comprises deamidation, the conversion of glutamine to glutamic acid or asparagine to aspartic acid.

1053. The method of claim 1010, wherein the one or more post translational modifications comprises modifications of a single amino acid or more than one i.e. in combinations.

1054. The method of claim 990, wherein the one or more antigenic peptides are obtained by digestion and/or proteolysis of natural proteins or proteins derived by in vitro translation of mRNA.

1055. The method of claim 990, wherein the sequence of the one or more antigenic peptides is determined by elution of peptides from the MHC binding groove of a naturally existing MHC complex.

1056. The method of claim 990, wherein the one or more antigenic peptides are derived from one or more recombinant sources.

1057. The method of claim 1056, wherein the one or more recombinant sources comprises one or more monomeric or multimeric peptides.

1058. The method of claim 1056, wherein the one or more recombinant sources comprises one or more parts of a bigger recombinant protein.

1059. The method of claim 1056, wherein the one or more recombinant sources comprises one or more MHC class 1 peptides P.

1060. The method of claim 1059, wherein the one or more MHC class 1 peptides P comprises Peptide-linker-β2m, β2m being full length or truncated.

1061 . The method of claim 1059, wherein the one or more MHC class 1 peptides P comprises Peptide-linker-MHC class 1 heavy chain, the heavy chain being full length or truncated.

1062. The method of claim 1059, wherein the one or more MHC class 1 peptides P comprises truncated class I heavy chain consisting of the extracellular part i.e the αi , α2, and α domains.

1063. The method of claim 1059, wherein the one or more MHC class 1 binding peptides comprises heavy chain fragment containing the α1 and α2 domains, or α1 domain alone, or any fragment or full length β2m or heavy chain attached to a designer domain(s) or protein fragment(s).

1064. The method of claim 1054, wherein the one or more recombinant sources comprises one or more MHC class 2 binding peptides.

1065. The method of claim 1054, wherein the one or more MHC class 2 binding peptides comprises Peptide-linker-MHC class 2 alpha-chain, full length or truncated.

1066. The method of claim 1054, wherein the one or more MHC class 2 binding peptides comprises Peptide-linker-MHC class 2 beta-chain, full length or truncated.

1067. The method of claim 1054, wherein the one or more MHC class 2 binding peptides comprises Peptide-linker-MHC class 2 α-chain-linker-MHC class 2 β-chain, both chains are full length or truncated, truncation involve, omission of α- and/or β- chain intermembrane domain, or omission of α- and/or β-chain intermembrane plus cytoplasmic domains.

1068. The method of claim 1054, wherein the one or more MHC class 2 binding peptides comprises MHC class 2 part of the construction consisting of fused domains from NH2-terminal, MHC class 2 β1domain-MHC class 2 α1 domain-constant α3 of MHC class 1 , or alternatively of fused domains from NH2-terminal, MHC class 2 α1domain-MHC class 2 β1 domain-constant α3 of MHC class 1.

1069. The method of claim 1054, wherein the one or more MHC class 2 binding peptides comprises β2m associated non-covalently in the folded MHC complex.

1070. The method of claim 1054, wherein the one or more MHC class 2 binding peptides comprises β2m covalently associated in the folded MHC class 2 complex.

1071. The method of claim 989, wherein the one or more peptides P are obtained from chemical synthesis.

1072. The method of claim 1071 , wherein the chemical synthesis comprises solid phase synthesis.

1073. The method of claim 1071 , wherein the chemical synthesis comprises fluid phase synthesis.

1074. The method of claim 1071 , wherein the chemical synthesis method comprises the further step of loading of the peptide into the functional MHC protein.

1075. The method of claim 1074, wherein the loading of the peptide into the functional MHC protein comprises the step of loading of free peptide P during the folding of the functional MHC protein and the formation of the MHC peptide complex.

1076. The method of claim 1074, wherein the loading of the peptide into the

MHCmer comprises that the peptide to be folded into the binding groove is encoded together with e.g. the α heavy chain or fragment hereof by a gene construct having the structure, heavy chain-flexible linker- peptide followed by folding in vitro.

1077. The method of claim 1074, wherein the loading of the peptide into the

MHCmer comprises that the peptide is part of a recombinant protein construct.

1078. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions.

1079. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions in solution.

1080. The method of claim 10674, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions "in situ".

1081 . The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions by aided exchange.

1082. The method of claim 1074, wherein the loading of the peptide into the

MHCmer comprises one or more exchange reactions by in vivo loading.

1083. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more in vitro exchange reactions where a peptide already in place in the binding groove are being exchanged by another peptide species.

1084. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions where the parent molecule is attached to other molecules, structures, surfaces, artificial or natural membranes and nano-particles.

1085. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions where the parent construct is made with a peptide containing a meta-stable amino acid analog that is split by either light or chemically induction thereby leaving the parent structure free for access of the desired peptide in the binding groove.

1086. The method of claim 1074 wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions comprising in vivo loading of MHC class I and Il molecules expressed on the cell surface with the desired peptides.

1087. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions comprising that cells are transfected by the peptides themselves or by the mother proteins that are then being processed leading to an in vivo analogous situation where the peptides are bound in the groove during the natural cause of MHC expression by the transfected cells.

1088. The method of claim 1074, wherein the loading of the peptide into the MHCmer comprises one or more exchange reactions comprising one or more professional antigen presenting cells e.g. dendritic cells, macrophages, or Langerhans cells.

1089. The method of claim 1074 further comprising a step to verify correct folding of the one or more MHC-peptide complexes.

1090. A composition comprising a plurality of MHC monomers and/or MHC multimers according to any of claims 1 to 797, wherein the MHC multimers are identical, and a carrier.

1091. A composition comprising a plurality of MHC monomers and/or MHC multimers according to any of claims 1 to 779, wherein the MHC multimers are different, and a carrier.

1092. A kit comprising a MHC monomer or a MHC multimer according to any of claims 1 to 797, or the composition according to any of claims 1090 and 1091 , and at least one additional component, such as a positive control and/or instructions for use.

1093. A method for immune monitoring one or more diseases comprising the following steps i) providing the MHC monomer or MHC multimer according to any of the claims 1 to 797, or the individual components thereof, ii) providing a population of T cells, and iii) measuring the number, activity or state of T cells specific for said MHC monomer or MHC multimer, thereby immune monitoring said one or more diseases.

1094. A method for diagnosing one or more diseases comprising the following steps: i) providing the MHC monomer or MHC multimer according to any of the claims 1 to

797, or individual components thereof, ii) providing a population T cells, and iii) measuring the number, activity or state T cells specific for said MHC monomer or

MHC multimer, thereby diagnosing said one or more diseases.

1095. A method for isolation of one or more antigen-specific T cells, said method comprising the steps of i) providing the MHC monomer or MHC multimer according to any of the claims 1 to 797, or individual components thereof, ii) providing a population of T cells, and iii) isolating T cells specific for said MHC monomer or MHC multimer.

1096. The method of any of claims 1093 to 1095, wherein the measurement of T cells comprises the step of counting of T cells specific for the said MHC monomer or MHC multimer.

1097. The method of any of claims 1093 to 1095, wherein the measurement of T cells comprises the step of sorting of T cells specific for said MHC monomer or MHC multimer.

1098. The method of any of claims 1093 to 1095, wherein the measurement of T cells comprises the step of isolation of T cells specific for the MHC monomer or MHC multimer.

1099. The method of any of claims 1093 to 1095, wherein the number of T cells specific for said MHC monomer or MHC multimer is determined from the number of T cells that are bound by more than a given threshold number of MHC multimers.

1 100. The method of claim 1099, wherein the amount of MHC multimers bound to an individual T cell is measured by flow cytometry.

1 101 . The method of claim 1099, where the amount of MHC multimers bound to an individual T cell is measured by microscopy.

1 102. The method of claim 1099 where the amount of MHC multimers bound to an individual T cell is measured by capture on solid support, optionally followed by elution of T cells.

1 103. The method of claim 1099 where the amount of MHC multimers bound to a population of T cells is determined by the total number of MHC multimers bound to the population of T cells.

1 104. The method of any of claims 1093 to 1095, where the T cells of step (ii) are immobilized.

1 105. The method of claim 1 104 where the MHC multimers are labelled, and the number of individual T cells specific for said MHC multimer is determined using microscopy.

1 106. The method of claim 1 104 where the MHC multimers are labelled, and the number of T cells specific for said MHC multimer is determined from the total signal of the population of T cells

1 107. The method of claims 1 104-1 106 where the immobilized T cells are part of a solid tissue

1108. The method of claim 1107 where the solid tissue is part of a living animal and the detection of T cells specific for the MHC multimer is performed in vivo.

1109. The method of claim 1108 where the detection of T cells involves magnetic resonance imaging or electron spin resonance scanning.

1110. The method any of claims 1093 to 1095, where the number, activity or state of the T cells specific for said MHC multimer are determined from the result of the interaction of said MHC multimer with said T cells.

1111. The method of claim 1110 where the result of the interaction of said MHC multimer with said T cells is an up- or down-regulation, such as an increased or decreased production, of the amount of a specific factor.

1112. The method of claim 1111 where the specific factor is a secreted soluble factor.

1113. The method of claim 1111 where the specific factor is an intracellular factor.

1114. The method of claim 1111 where the specific factor is a mRNA

1115. The method of claim 1111 where the specific factor is a cytokine

1116. The method of claim 1111 where the specific factor is a surface receptor of T cells

1117. The method of claim 1111 where the specific factor is a secreted soluble factor that is captured on solid support.

1118. The method of claim 1111 where the specific factor is interferon-gamma (INF-gamma).

1119. The method of claims 1110-1118 where individual T cells are measured.

1 120. The method of claims 1 1 10-1 1 18 where populations of T cells are measured.

1 121 . The method of claims 1 1 10-1 1 18 where the specific factor is immobilized on solid support before measurement of its amount.

1 122. The method of claims 1 1 10-1 1 18 where the specific factor is in solution when its amount is measured.

1 123. The method of claim 1 1 10 where the result of the interaction of said MHC multimer with the T cell is T cell apoptosis.

1 124. The method of claim 1 1 10 where the result of the interaction of said MHC multimer with the T cell is T cell apoptosis.

1 125. The method of claim 1 1 10 where the result of the interaction of said MHC multimer with the T cell is T cell differentiation.

1 126. The method of claim 1 1 10 where the result of the interaction of said MHC multimer with the T cell is T cell inactivation.

1 127. The method of claim 1 1 10 where the measurement of T cells specific for said MHC multimer with the T cell involves flow cytometry.

1 128. The method of any of claims 1093 to 1095, wherein the measurement of antigen-specific T cells comprises flow cytometry.

1 129. The method of any of claims 1093 to 1095, wherein the measurement of antigen-specific T cells comprises indirect measurement of individual T cells.

1 130. The method of any of claims 1093 to 1095, wherein the measurement of antigen-specific T cells comprises indirect measurement of populations of T cells.

1 131 . The method of claims 1 1 10-1 130 where in step (i) an antigenic peptide is added instead of a MHC monomer or MHC multimer.

1 132. The method of any of claims 1093 to 1095, wherein the measurement of antigen-specific T cells involves immunohistochemistry (IHC).

1 133. The method of any of claims 1093 to 1095, wherein the measurement of antigen-specific T cells involves limited dilution assay (LDA).

1 134. The method of any of claims 1093 to 1095, wherein the measurement of antigen-specific T cells involves microscopy.

1 135. The method of any of claims 1093 to 1095, wherein the method comprises the step of detecting one or more marker molecules associated with the MHC monomer or MHC multimer.

1 136. The method of claim 1 135, wherein the one or more marker molecules comprises one or more antibodies and/or antibody fragments.

1 137. The method of claim 1 135, wherein the one or more marker molecules comprises one or more aptamers.

1 138. The method of claim 1 135, wherein the one or more marker molecules comprises one or more proteins.

1 139. The method of claim 1 135, wherein the one or more marker molecules comprises one or more peptides.

1 140. The method of claim 1 135, wherein the one or more marker molecules comprises one or more small organic molecules.

1 141. The method of claim 1 135, wherein the one or more marker molecules comprises one or more natural compounds such as one or more steroids.

1 142. The method of claim 1 135, wherein the one or more marker molecules comprises one or more non-peptide polymers.

1 143. The method of any of the claims 1093 to 1095, wherein the method comprises the further step of providing one or more labelling molecules.

1 144. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more labelling molecules that results in directly detectable T cells.

1 145. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more labelling molecules that results in indirectly detectable T cells.

1 146. The method of claim 1 143, wherein the one or more labelling molecules are attached via one or more linkers.

1 147. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more fluorescent labels.

1 148. The method of claim 1 147, wherein the one or more fluorescent labels can be selected from the group consisting of 5-(and 6)-carboxyfluorescein, 5- or 6- carboxyfluorescein, 6-(fluorescein)-5-(and 6)-carboxamido hexanoic acid, fluorescein isothiocyanate (FITC), rhodamine, tetramethylrhodamine, and dyes such as Cy2, Cy3, and Cy5, optionally substituted coumarin including AMCA, PerCP, phycobiliproteins including R-phycoerythrin (RPE) and allophycoerythrin (APC), Texas Red, Princeston Red, Green fluorescent protein (GFP) and analogues thereof, and conjugates of R-phycoerythrin or allophycoerythrin and e.g. Cy5 or Texas Red, and inorganic fluorescent labels based on semiconductor nanocrystals (like quantum dot and Qdot™ nanocrystals), and time-resolved fluorescent labels based on lanthanides like Eu3+ and Sm3+.

1 149. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more enzyme labels.

1 150. The method of claim 1 149, wherein the one or more enzyme labels can be selected from the group consisting of horse radish peroxidase (HRP), alkaline phosphatase (AP), beta-galactosidase (GAL), glucose-6-phosphate dehydrogenase, beta-N-acetylglucosaminidase, β-glucuronidase, invertase, Xanthine Oxidase, firefly luciferase and glucose oxidase (GO),

1 151 . The method of claim 1 143, wherein the one or more labelling molecules comprises one or more radioisotopes.

1 152. The method of claim 1 151 , wherein the one or more radioisotopes can be selected from the group consisting of isotopes of iodide, cobalt, selenium, tritium, and/or phosphor.

1 153. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more chemiluminescent labels.

1 154. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more luminescent labels.

1 155. The method of claim 1 154, wherein the one or more luminescent labels can be selected from the group consisting of luminol, isoluminol, acridinium esters, 1 ,2-dioxetanes and pyridopyridazines.

1 156. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more polymers.

1 157. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more metal particles.

1 158. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more haptens.

1 159. The method of claim 1 158, wherein the one or more haptens can be selected from the group consisting of DNP, biotin, and digoxiginin.

1 160. The method of claim 1 143, wherein the one or more labelling molecules comprises one or more antibodies.

1 161 . The method of claim 1 143, wherein the one or more labelling molecules comprises one or more dyes.

1 162. The method of any of the claims 1093 to 1095, wherein the method comprises a detection step based on flow cytometry or flow cytometry-like analysis.

1 163. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of TCRs attached to a lipid bilayer.

1 164. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of one or more individual T cells in a fluid sample.

1 165. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of one or more populations of T cells in a fluid sample.

1 166. The method of claim 1 164, where in the direct detection of one or more individual T cells in a fluid sample comprises flow cytometry.

1 167. The method of claim 1 164, where in the direct detection of one or more populations of T cells in a fluid sample comprises flow cytometry.

1 168. The method of claim 1 164, where in the direct detection of one or more individual T cells in a fluid sample comprises microscopy.

1 169. The method of claim 1 164, where in the direct detection of one or more populations of T cells in a fluid sample comprises microscopy.

1 170. The method of claim 1 164, where in the direct detection of one or more individual T cells in a fluid sample comprises capture of T cells on a solid support followed by elution of said T cells.

1 171. The method of claim 1 164, where in the direct detection of one or more populations of T cells in a fluid sample comprises capture of T cells on a solid support followed by elution of said T cells.

1 172. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of one or more individual immobilized T cells.

1 173. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of one or more populations of immobilized T cells.

1 174. The method of claim 1 172, wherein one or more individual T cells are immobilized on a solid support such as particles, beads, biodegradable particles, sheets, gels, filters, membranes, nylon membranes, fibres, capillaries, needles, microtitre strips, tubes, plates, wells, combs, pipette tips, micro arrays, chips and slides.

1 175. The method of claim 1 173, wherein one or more populations of T cells are immobilized on a solid support such as particles, beads, biodegradable particles, sheets, gels, filters, membranes, nylon membranes, fibres, capillaries, needles, microtitre strips, tubes, plates, wells, combs, pipette tips, micro arrays, chips and slides.

1 176. The method of claim 1 172, wherein the immobilization of one or more individual T cells are directly immobilized on the solid support.

1 177. The method of claim 1 173, wherein the immobilization of one or more populations of T cells are directly immobilized on the solid support.

1 178. The method of claim 1 172, wherein the immobilization of one or more individual T cells are directly immobilized through a linker on the solid support.

1 179. The method of claim 1 173, wherein the immobilization of one or more individual T cells are directly immobilized through a linker on the solid support.

1 180. The method of any of the claims 1093 to 1095, wherein the direct detection of one or more individual immobilized T cells comprises phenotyping a T cell sample using MHC multimer beads.

1 181 . The method of any of the claims 1093 to 1095, wherein the direct detection of one or more populations of immobilized T cells comprises phenotyping a T cell sample using MHC multimer beads.

1 182. The method of any of the claims 1093 to 1095, wherein the direct detection of one or more individual immobilized T cells comprises detection of T cells immobilized to solid support in a defined pattern.

1 183. The method of any of the claims 1093 to 1095, wherein the direct detection of one or more populations of immobilized T cells comprises detection of T cells immobilized to solid support in a defined pattern.

1 184. The method of any of claims 1 180 to 1 183, wherein the direct detection of one or more individual immobilized T cells is followed by sorting of said T cells.

1 185. The method of any of claims 1 180 to 1 183, wherein the direct detection one or more populations of immobilized T cells is followed by sorting of said T cells.

1 186. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of one or more individual T cells in a solid tissue either in vitro or in vivo.

1 187. The method of any of the claims 1093 to 1095, wherein the method comprises direct detection of one or more populations of T cells in a solid tissue either in vitro or in vivo.

1 188. The method of any of the claims 1093 to 1095, wherein the method comprises indirect detection of one or more populations of T cells in a sample.

1 189. The method of any of the claims 1093 to 1095, wherein the method comprises indirect detection of one or more individual T cells in a sample.

1 190. The method of any of the claims 1093 to 1095, wherein the method comprises indirect detection of one or more individual T cells in a sample by measurement of activation.

1 191 . The method of any of the claims 1093 to 1095, wherein the method comprises indirect detection of one or more populations of T cells in a sample by measurement of activation.

1 192. The method of 1 191 , wherein the indirect detection of one or more individual T cells in a sample comprises measurement of secretion of soluble factors.

1 193. The method of 1 191 , wherein the indirect detection of one or more populations of T cells in a sample comprises measurement of secretion of soluble factors.

1 194. The method of claim 1 192, wherein the measurement of secretion of soluble factors comprises measurement of extracellular secreted soluble factors.

1 195. The method of claim 1 194, wherein the measurement of extracellular secreted soluble factors comprises analysis of a fluid sample.

1 196. The method of claim 1 194, wherein the measurement of extracellular secreted soluble factors comprises detection of T cells by capture of extracellular secreted soluble factor on a solid support.

1 197. The method of claim 1 194, wherein the measurement of extracellular secreted soluble factors comprises detection of T cells immobilized to solid support in a defined pattern.

1 198. The method of claim 1 194, wherein the measurement of extracellular secreted soluble factors comprises detection of T cells by measurement of effect of extracellular secreted soluble factor.

1 199. The method of claim 1 193, wherein the measurement of secretion of soluble factors comprises measurement of intracellular secreted soluble factors.

1200. The method of 1 192, wherein the indirect detection of individual or populations of T cells in a sample comprises measurement of expression of one or more receptors.

1201 . The method of 1 192, wherein the indirect detection of individual or populations of T cells in a sample comprises measurement of T cell effector function.

1202. The method of any of the claims 1093 to 1095, wherein the method comprises indirect detection of individual or populations of T cells in a sample by measurement of T cell proliferation.

1203. The method of any of the claims 1093 to 1095, wherein the method comprises indirect detection of individual or populations of T cells in a sample by measurement of T cell inactivation such as measuremet of blockage of TCR and/or measurement of induction of apoptosis.

1204. The method of any of the claims 1093 to 1095, wherein the method comprises immunohistochemistry or immunohistochemistry-like analysis.

1205. The method of any of claims 1093 to 1095, wherein the method comprises a measuring step involving an ELISA or ELISA-like analysis.

1206. The method of any of the claims 1093 to 1095, wherein the antigen- specific T cells are isolated using MHC multimers immobilized on a solid phase.

1207. The method of claim 1206, wherein the solid phase is a bead.

1208. The method of claim 1206, wherein the solid phase is immunotubes.

1209. The method of claim 1206, wherein the solid phase is microtiter plates.

1210. The method of claim 1206, wherein the solid phase is microchips.

121 1 . The method of claim 1206, wherein the solid phase is microarrays.

1212. The method of claim 1206, wherein the solid phase is test strips.

1213. The method of any of the claims 1093 to 1095, wherein the method comprises blocking of the sample with a protein solution such as BSA or skim milk.

1214. The method of any of the claims 1093 to 1095, wherein the method comprises blocking of the MHC multimer with a protein solution such as BSA or skim milk.

1215. The method of any of the claims 1093 to 1095, wherein the method comprises mixing of MHC multimer coated beads with the cell sample.

1216. The method of any of the claims 1093 to 1095, wherein the method comprises incubation of MHC multimer coated beads with the cell sample.

1217. The method of any of the claims 1093 to 1095, wherein the method comprises a washing step after incubation of MHC multimer coated beads with the cell sample.

1218. The method of any of the claims 1093 to 1095, wherein the method comprises release of the immobilized T cells from the beads.

1219. The method of claim 1218, wherein the T cells are released by cleavage of the linker.

1220. The method of claim 1218, wherein the T cells are released by changing the pH.

1221 . The method of claim 1218, wherein the T cells are released by changing the salt concentration.

1222. The method of claim 1218, wherein the T cells are released by addition of one or more competitive binders.

1223. The method of claim 1218, wherein the T cells are released by a method that does not disrupts the integrity of the cells.

1224. The method of any of the claims 1093 to 1095, wherein the method comprises manipulation of the T cells after release from the beads.

1225. The method of claim 1224, wherein the manipulation is induction of apoptosis.

1226. The method of claim 1224, wherein the manipulation is induction of proliferation.

1227. The method of claim 1224, wherein the manipulation is induction of differentiation.

1228. The method of any of the claims 1093 to 1095, wherein the sample to be analysed is acquired from the bone marrow, the blood, the lymph, a solid tissue sample or a suspension of a tissue sample.

1229. The method of any of the claims 1093 to 1095, wherein the sample to be analysed is acquired from solid tissue, solid tissue section or a fluid such as whole blood, serum, plasma, nasal secretions, sputum, urine, sweat, saliva, transdermal exudates, pharyngeal exudates, bronchoalveolar lavage, tracheal aspirations, cerebrospinal fluid, synovial fluid, fluid from joints, vitreous fluid, vaginal or urethral secretions, or disaggregated cellular tissues such as hair, skin, synovial tissue, tissue biopsies and nail scrapings.

1230. The method of any of the claims 1093 to 1095, wherein the sample to be analysed is acquired from cell suspensions derived from tissue samples or cell lines.

1231 . The method of claims 1093 to 1095 for use in a method of diagnosing an infection in an individual, such as an animal, for example a human being.

1232. The method of claims 1231 , wherein the infection is caused by a bacteria.

1233. The method of claims 1231 , wherein the infection is caused by a virus.

1234. The method of claims 1231 , wherein the infection is caused by a parasite.

1235. The method of claims 1231 , wherein the infection is caused by a worm.

1236. The method of claims 1231 , wherein the infection is caused by an insect.

1237. The method of claims 1231 , wherein the infection is caused by a protozoan.

1238. The method of claims 1231 , wherein the infection is caused by a Flagellate.

1239. The method of claims 1231 , wherein the infection is caused by

Entamoeba histolytica.

1240. The method of claims 1231 , wherein the infection is caused by Giardia.

1241 . The method of claims 1231 , wherein the infection is caused by

Trichomonas.

1242. The method of claims 1231 , wherein the infection is caused by Leishmania.

1243. The method of claims 1231 , wherein the infection is caused by Trypanosomes.

1244. The method of claims 1231 , wherein the infection is caused by Trichomonas vaginalis.

1245. The method of claims 1231 , wherein the infection is caused by Plasmodium.

1246. The method of claims 1231 , wherein the infection is caused by an archaeo.

1247. The method of claims 1231 , wherein the infection is by a fungus.

1248. The method of claims 1093 to 1095 for use in diagnosis of allergic disease in an individual, such as an animal, for example a human being.

1249. The method of claims 1093 to 1095 for use in diagnosis of transplantation related disease.

1250. The method of claims 1093 to 1095 for use in diagnosis of autoimmune disease in an individual, such as an animal, for example a human being.

1251 . The method of claims 1093 to 1095 for use in diagnosis of inflammation in an individual, such as an animal, for example a human being.

1252. The method of any of the claims 1093 to 1095 for use in monitoring of an adaptive immune response in an individual, such as an animal, for example a human being.

1253. The method of any of the claims 1093 to 1095 for use in monitoring of autoimmune diseases in an individual, such as an animal, for example a human being.

1254. The method of any of the claims 1093 to 1095 for use in diagnosis of autoimmune diseases in an individual, such as an animal, for example a human being.

1255. The method of any of the claims 1093 to 1095 for use in determining the prognosis of autoimmune diseases in an individual, such as an animal, for example a human being.

1256. The method of any of the claims 1093 to 1095 for use in development of vaccines for autoimmune diseases in an individual, such as an animal, for example a human being.

1257. The method of any of the claims 1093 to 1095 for use in development of therapy for autoimmune diseases in an individual, such as an animal, for example a human being.

1258. The method of any of the claims 1093 to 1095 for use in determination of the prognosis for diseases associated with the immune system in an individual, such as an animal, for example a human being.

1259. The method of any of the claims 1093 to 1095 for use in diagnostics for diseases associated with the immune system in an individual, such as an animal, for example a human being.

1260. The method of any of the claims 1093 to 1095 for use in monitoring a vaccine response in an individual, such as an animal, for example a human being.

1261. The method of any of the claims 1093 to 1095 for use in development of a vaccine in an individual, such as an animal, for example a human being.

1262. The method of any of the claims 1093 to 1095 for use of determination of the overall wellness of the immune system in an individual, such as an animal, for example a human being.

1263. The method of any of the claims 1093 to 1095 for use of determination of the efficacy of an anti HIV treatment in an individual, such as an animal, for example a human being.

1264. The method of any of the claims 1093 to 1095 for use of development of an anti HIV treatment in an individual, such as an animal, for example a human being. o

1265. The method of any of the claims 1093 to 1095, wherein T cells specific for Cytomegalovirus (CMV) are detected.

1266. The method of any of the claims 1093 to 1095, wherein T cells specific for allergens are detected.

1267. The method of any of the claims 1093 to 1095, wherein T cells specific for auto antigens are detected.

1268. The method of any of the claims 1093 to 1095, wherein T cells specific for disease associated proteins are detected.

1269. The method of any of the claims 1093 to 1095, wherein the measuring of said T cells is predictive of the outcome of an organ transplantation in an individual, such as an animal, for example a human being.

1270. The method of any of the claims 1093 to 1095, wherein the outcome of an organ transplantation is monitored in an individual, such as an animal, for example a human being.

1271 . The method of any of the claims 1093 to 1095, wherein the method is used for adjustment of an immune suppressive treatment in an individual, such as an animal, for example a human being.

1272. The method of any of the claims 1093 to 1095, wherein the method is used for monitoring an immune suppressive treatment in an individual, such as an animal, for example a human being.

1273. The method of any of the claims 1093 to 1095, wherein the method is used for monitoring an immune suppressive treatment in an individual, such as an animal, for example a human being.

1274. The method of any of the claims 1093 to 1095, wherein the method comprises the provision of one or more derivatives of MHC multimers.

1275. The method of any of the claims 1093 to 1095, wherein the said method comprises detection of and/or analysis of and/or manipulation of one or more specific T cells with MHC multimers comprising antigenic peptides from antigens selected from the group consisting of Adenovirus (subgropus A-F), BK-virus, CMV (Cytomegalo virus, HHV-5), EBV (Epstein Barr Virus, HHV-4), HBV (Hepatitis B

Virus), HCV (Hepatitis C virus), HHV-6a and b (Human Herpes Virus-6a and b), HHV-7, HHV-8, HSV-1 (Herpes simplex virus-1 , HHV-1 ), HSV-2 (HHV-2), JC-virus, SV-40 (Simian virus 40), VZV (Varizella-Zoster-Virus, HHV-3), Parvovirus B19, Haemophilus influenza, HIV-1 (Human immunodeficiency Virus-1 ), HTLV-1 (Human T-lymphotrophic virus-1 ), HPV (Human Papillomavirus), Mycobacterium tuberculosis,

Mycobacterium bovis, Borrelia burgdorferi, Helicobacter pylori, Streptococcus pneumoniae, Listeria monocytogenes, Histoplasma capsulatum, Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Pneumocystis carinii, Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Schistosoma mansoni, Schistosoma japonicum, Schistosoma haematobium, Trypanosoma cruzi,

Trypanosoma rhodesiense, Trypanosoma gambiense, Leishmania donovani, Leishmania tropica, Birch, Hazel, Elm, Ragweed, Wormwood, Grass, Mould and Dust Mite.

1276. The method of any of the claims 1093 to 1095, wherein the said method comprises detection of and/or analysis of and/or manipulation of one or more specific T cells with MHC multimers comprising antigenic peptides from antigens selected from the group consisting of HA-1 , HA-8, USP9Y, SMCY, TPR-protein, HB-1 Y, GAD64, Collagen, Survivin, Survivin-2B, Livin/ML-IAP, Bcl-2, Mcl-1 , BcI-X(L), Mucin- 1 , NY-ESO-1 , Telomerase, CEA, MART-1 , HER-2/neu, bcr-abl, PSA, PSCA,

Tyrosinase, p53, hTRT, Leukocyte Proteinase-3, hTRT, gpl OO, MAGE antigens, GASC, JMJD2C, JARD2 (JMJ), JHDM3a, WT-1 ,CA 9, Protein kinases, Collagen, human cartilage glycoprotein 39, beta-amyloid, Abeta42, APP, Presenilin 1 .

1277. A method for performing a vaccination of an individual in need thereof, said method comprising the steps of

providing a MHC monomer or a MHC multimer according to any of the claims 1 to 797, or the individual components thereof, and administering said MHC monomer or MHC multimer to said individual and obtaining a protective immune response, thereby performing a vaccination of the said individual.

1278. The method according to claim 1277, wherein the MHC multimer is administered alone to said individual.

1279. The method according to claim 1277, wherein the MHC multimer is administered together with one or more adjuvant(s) to said individual.

1280. The method according to claim 1277, wherein the MHC multimer is administered to said individual more than once such as twice or three times.

1281. The method according to claim 1277, wherein the MHC multimer is administered to said individual cutaneously.

1282. The method according to claim 1277, wherein the MHC multimer is administered to said individual subcutaneously (SC).

1283. The method according to claim 1277, wherein the MHC multimer is administered to said individual by intramuscular (IM) administration.

1284. The method according to claim 1277, wherein the MHC multimer is administered to said individual by intravenous (IV) administration.

1285. The method according to claim 1277, wherein the MHC multimer is administered to said individual by Per-oral (PO) administration.

1286. The method according to claim 1277, wherein the MHC multimer is administered to said individual inter peritoneally.

1287. The method according to claim 1277, wherein the MHC multimer is administered to said individual pulmonally.

1288. The method according to claim 1277, wherein the MHC multimer is administered to said individual vaginally.

1289. The method according to claim 1277, wherein the MHC multimer is administered to said individual rectally.

1290. The method according to claim 1277, wherein the vaccination is a therapeutic vaccination i.e. vaccination "teaching" the immune system to fight an existing infection or disease.

1291 . A method for performing therapeutic treatment of an individual comprising the steps of

Providing the MHC multimer according to claim 1 to 797, or individual components thereof, and

Isolating or obtaining T-cells from a source, such as an individual or an ex-vivo library or cell bank, wherein said isolated or obtained T-cells are specific for said provided MHC multimer,

Optionally manipulating said T-cells, and

Introducing said isolated or obtianed T-cells into an individual to be subjected to a therapeutic treatment, wherein the individual can be the same individual or a different individual from the source individual.

1292. A method for immune monitoring one or more cancer diseases comprising the step of monitoring one or more cancer antigen-specific T-cells, said method comprising the steps of

providing a MHC monomer or MHC multimer, or individual components thereof, according to any of the claims 1 to 797, providing a population of cancer antigen-specific T cells, or individual cancer antigen- specific T cells, and measuring the number and/or presence of cancer antigen-specific T cells specific for the peptide P of the MHC monomer or MHC multimer, thereby immune monitoring said one or more cancer diseases.

1293. A method for diagnosing one or more cancer diseases comprising immune monitoring of cancer antigen-specific T cells, said method comprising the steps of

providing the MHC multimer or individual components thereof according to any of the claims 1 to 797,

providing a population of cancer antigen-specific T cells, or individual cancer antigen- specific T cells, and

measuring the number and/or presence of T cells specific for the peptide P of the

MHC monomer or MHC multimer, thereby diagnosing said one or more cancer diseases.

1294. The method of any of claims 1292 and 1293, wherein the monitoring of antigen-specific T cells comprises detection of antigen-specific T cells specific for the peptide P of the MHC multimer.

1295. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises counting of antigen-specific T cells specific for the peptide P of the MHC multimer.

1296. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises sorting of antigen-specific T cells specific for the peptide P of the MHC multimer.

1297. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises isolation of antigen-specific T cells specific for the peptide P of the MHC multimer.

1298. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises flow cytometry.

1299. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises ELISPOT.

1300. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises LDA.

1301 . The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises QUantaferon-like analysis.

1302. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises IHC.

1303. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises LDA.

1304. The method of any of claims 1292 and 1293, wherein the measurement of antigen-specific T cells comprises microscopy.

1305. The method of any of the claims 1292 and 1293, wherein the method comprises one or more marker molecules in addition to the MHC multimer.

1306. The method of claim 1305, wherein the one or more marker molecules comprises one or more antibodies and/or antibody fragments.

1307. The method of claim 1305, wherein the one or more marker molecules comprises one or more aptamers.

1308. The method of claim 1305, wherein the one or more marker molecules comprises one or more proteins.

1309. The method of claim 1305, wherein the one or more marker molecules comprises one or more peptides.

1310. The method of claim 1305, wherein the one or more marker molecules comprises one or more small organic molecules.

131 1 . The method of claim 1305, wherein the one or more marker molecules comprises one or more natural compounds such as one or more steroids.

1312. The method of claim 1305, wherein the one or more marker molecules comprises one or more non-peptide polymers.

1313. The method of any of the claims 1292 and 1293, wherein the method comprises one or more labelling molecules.

1314. The method of claim 1313, wherein the one or more labelling molecules comprises one or more labelling molecules that results in directly detectable T cells.

1315. The method of claim 1313, wherein the one or more labelling molecules comprises one or more labelling molecules that results in indirectly detectable T cells.

1316. The method of claim 1313, wherein the one or more labelling molecules are attached via one or more linkers.

1317. The method of claim 1313, wherein the one or more labelling molecules comprises one or more fluorescent labels.

1318. The method of claim 1317, wherein the one or more fluorescent labels can be selected from the group consisting of 5-(and 6)-carboxyfluorescein, 5- or 6- carboxyfluorescein, 6-(fluorescein)-5-(and 6)-carboxamido hexanoic acid, fluorescein isothiocyanate (FITC), rhodamine, tetramethylrhodamine, and dyes such as Cy2, Cy3, and Cy5, optionally substituted coumarin including AMCA, PerCP, phycobiliproteins including R-phycoerythrin (RPE) and allophycoerythrin (APC), Texas Red, Princeston Red, Green fluorescent protein (GFP) and analogues thereof, and conjugates of R-phycoerythrin or allophycoerythrin and e.g. Cy5 or Texas Red, and inorganic fluorescent labels based on semiconductor nanocrystals (like quantum dot and Qdot™ nanocrystals), and time-resolved fluorescent labels based on lanthanides like Eu3+ and Sm3+.

1319. The method of claim 1313, wherein the one or more labelling molecules comprises one or more enzyme labels.

1320. The method of claim 1319, wherein the one or more enzyme labels can be selected from the group consisting of horse radish peroxidase (HRP), alkaline phosphatase (AP), beta-galactosidase (GAL), glucose-6-phosphate dehydrogenase, beta-N-acetylglucosaminidase, β-glucuronidase, invertase, Xanthine Oxidase, firefly luciferase and glucose oxidase (GO),

1321. The method of claim 1313, wherein the one or more labelling molecules comprises one or more radioisotopes.

1322. The method of claim 1321 , wherein the one or more radioisotopes can be selected from the group consisting of isotopes of iodide, cobalt, selenium, tritium, and/or phosphor.

1323. The method of claim 1313, wherein the one or more labelling molecules comprises one or more chemiluminescent labels.

1324. The method of claim 1313, wherein the one or more labelling molecules comprises one or more luminescent labels.

1325. The method of claim 1324, wherein the one or more luminescent labels can be selected from the group consisting of luminol, isoluminol, acridinium esters, 1 ,2-dioxetanes and pyridopyridazines.

1326. The method of claim 1313, wherein the one or more labelling molecules comprises one or more polymers.

1327. The method of claim 1313, wherein the one or more labelling molecules comprises one or more metal particles.

1328. The method of claim 1313, wherein the one or more labelling molecules comprises one or more haptens.

1329. The method of claim 1328, wherein the one or more haptens can be selected from the group consisting of DNP, biotin, and digoxiginin.

1330. The method of claim 1313, wherein the one or more labelling molecules comprises one or more antibodies.

1331 . The method of claim 1313, wherein the one or more labelling molecules comprises one or more dyes.

1332. The method of any of the claims 1292 and 1293, wherein the method comprises flow cytometry or flow cytometry- 1 ike analysis.

1333. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of TCRs attached to a lipid bilayer.

1334. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of one or more individual T cells in a fluid sample.

1335. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of one or more populations of T cells in a fluid sample.

1336. The method of claim 1335, where in the direct detection of one or more individual T cells in a fluid sample comprises flow cytometry.

1337. The method of claim 1335, where in the direct detection of one or more populations of T cells in a fluid sample comprises flow cytometry.

1338. The method of claim 1335, where in the direct detection of one or more individual T cells in a fluid sample comprises microscopy.

1339. The method of claim 1335, where in the direct detection of one or more populations of T cells in a fluid sample comprises microscopy.

1340. The method of claim 1335, where in the direct detection of one or more individual T cells in a fluid sample comprises capture of T cells on a solid support followed by elution of said T cells.

1341 . The method of claim 1335, where in the direct detection of one or more populations of T cells in a fluid sample comprises capture of T cells on a solid support followed by elution of said T cells.

1342. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of one or more individual immobilized T cells.

1343. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of one or more populations of immobilized T cells.

1344. The method of claim 1342, wherein one or more individual T cells are immobilized on a solid support such as particles, beads, biodegradable particles, sheets, gels, filters, membranes, nylon membranes, fibres, capillaries, needles, microtitre strips, tubes, plates, wells, combs, pipette tips, micro arrays, chips and slides.

1345. The method of claim 1343, wherein one or more populations of T cells are immobilized on a solid support such as particles, beads, biodegradable particles, sheets, gels, filters, membranes, nylon membranes, fibres, capillaries, needles, microtitre strips, tubes, plates, wells, combs, pipette tips, micro arrays, chips and slides.

1346. The method of claim 1342, wherein the immobilization of one or more individual T cells are directly immobilized on the solid support.

1347. The method of claim 1343, wherein the immobilization of one or more populations of T cells are directly immobilized on the solid support.

1348. The method of claim 1342, wherein the immobilization of one or more individual T cells are directly immobilized through a linker on the solid support.

1349. The method of claim 1342, wherein the immobilization of one or more individual T cells are directly immobilized through a linker on the solid support.

1350. The method of any of the claims 1292 and 1293, wherein the direct detection of one or more individual immobilized T cells comprises phenotyping a T cell sample using MHC multimer beads.

1351 . The method of any of the claims 1292 and 1293, wherein the direct detection of one or more populations of immobilized T cells comprises phenotyping a

T cell sample using MHC multimer beads.

1352. The method of any of the claims 1292 and 1293, wherein the direct detection of one or more individual immobilized T cells comprises detection of T cells immobilized to solid support in a defined pattern.

1353. The method of any of the claims 1292 and 1293, wherein the direct detection of one or more populations of immobilized T cells comprises detection of T cells immobilized to solid support in a defined pattern.

1354. The method of any of claims 1350 to 1353, wherein the direct detection of one or more individual immobilized T cells is followed by sorting of said T cells.

1355. The method of any of claims 1350 to 1353, wherein the direct detection one or more populations of immobilized T cells is followed by sorting of said T cells.

1356. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of one or more individual T cells in a solid tissue either in vitro or in vivo.

1357. The method of any of the claims 1292 and 1293, wherein the method comprises direct detection of one or more populations of T cells in a solid tissue either in vitro or in vivo.

1358. The method of any of the claims 1292 to 1293, wherein the method comprises indirect detection of one or more populations of T cells in a sample.

1359. The method of any of the claims 1292 to 1293, wherein the method comprises indirect detection of one or more individual T cells in a sample.

1360. The method of any of the claims 1292 to 1293, wherein the method comprises indirect detection of one or more individual T cells in a sample by measurement of activation.

1361 . The method of any of the claims 1292 to 1293, wherein the method comprises indirect detection of one or more populations of T cells in a sample by measurement of activation.

1362. The method of claims 1359 and 1360, wherein the indirect detection of one or more individual T cells in a sample comprises measurement of secretion of soluble factors.

1363. The method of claims 1358 and 1361 , wherein the indirect detection of one or more populations of T cells in a sample comprises measurement of secretion of soluble factors.

1364. The method of claims 1362 and 1363, wherein the measurement of secretion of soluble factors comprises measurement of extracellular secreted soluble factors.

1365. The method of claim 1364, wherein the measurement of extracellular secreted soluble factors comprises analysis of a fluid sample.

1366. The method of claim 1364, wherein the measurement of extracellular secreted soluble factors comprises detection of T cells by capture of extracellular secreted soluble factor on a solid support.

1367. The method of claim 1364, wherein the measurement of extracellular secreted soluble factors comprises detection of T cells immobilized to solid support in a defined pattern.

1368. The method of claim 1364, wherein the measurement of extracellular secreted soluble factors comprises detection of T cells by measurement of effect of extracellular secreted soluble factor.

1369. The method of claim 1363, wherein the measurement of secretion of soluble factors comprises measurement of intracellular secreted soluble factors.

1370. The method of 1362, wherein the indirect detection of individual or populations of T cells in a sample comprises measurement of expression of one or more receptors.

1371. The method of 1362, wherein the indirect detection of individual or populations of T cells in a sample comprises measurement of T cell effector function.

1372. The method of any of the claims 1292 to 1293, wherein the method comprises indirect detection of individual or populations of T cells in a sample by measurement of T cell proliferation.

1373. The method of any of the claims 1292 to 1293, wherein the method comprises indirect detection of individual or populations of T cells in a sample by measurement of T cell inactivation such as measuremet of blockage of TCR and/or measurement of induction of apoptosis.

1374. The method of any of the claims 1292 to 1293, wherein the method comprises immunohistochemistry or immunohistochemistry-like analysis.

1375. The method of any of claims 1292 and 1293, wherein the method comprises ELISA or ELISA-like analysis.

1376. The method of any of the claims 1292 to 1293, wherein the antigen- specific T cells are isolated using MHC multimers immobilized on a solid surface.

1377. The method of claim 1376, wherein the solid phase is beads.

1378. The method of claim 1376, wherein the solid phase is immunotubes.

1379. The method of claim 1376, wherein the solid phase is microtiter plates.

1380. The method of claim 1376, wherein the solid phase is microchips.

1381 . The method of claim 1376, wherein the solid phase is microarrays.

1382. The method of claim 1376, wherein the solid phase is test strips.

1383. The method of any of the claims 1292 to 1293, wherein the method comprises blocking of the sample with a protein solution such as BSA or skim milk.

1384. The method of any of the claims 1292 to 1293, wherein the method comprises blocking of the MHC multimer with a protein solution such as BSA or skim milk.

1385. The method of any of the claims 1292 to 1293, wherein the method comprises mixing of MHC multimer coated beads with the cell sample.

1386. The method of any of the claims 1292 to 1293, wherein the method comprises incubation of MHC multimer coated beads with the cell sample.

1387. The method of any of the claims 1292 to 1293, wherein the method comprises a washing step after incubation of MHC multimer coated beads with the cell sample.

1388. The method of any of the claims 1292 to 1293, wherein the method comprises release of the immobilized T cells from the beads.

1389. The method of claim 1388, wherein the T cells are released by cleavage of the linker.

1390. The method of claim 1388, wherein the T cells are released by changing the pH.

1391 . The method of claim 1388, wherein the T cells are released by changing the salt concentration.

1392. The method of claim 1388, wherein the T cells are released by addition of one or more competitive binders.

1393. The method of claim 1388, wherein the T cells are released by a method that does not disrupts the integrity of the cells.

1394. The method of any of the claims 1292 to 1293, wherein the method comprises manipulation of the T cells after release from the beads.

1395. The method of claim 1394, wherein the manipulation is induction of apoptosis.

1396. The method of claim 1394, wherein the manipulation is induction of proliferation.

1397. The method of claim 1394, wherein the manipulation is induction of differentiation.

1398. The method of any of the claims 1292 to 1293, wherein the sample to be analysed is acquired from the bone marrow, the blood, the lymph, a solid tissue sample or a suspension of a tissue sample.

1399. The method of any of the claims 1292 to 1293, wherein the sample to be analysed is acquired from solid tissue, solid tissue section or a fluid such as whole blood, serum, plasma, nasal secretions, sputum, urine, sweat, saliva, transdermal exudates, pharyngeal exudates, bronchoalveolar lavage, tracheal aspirations, cerebrospinal fluid, synovial fluid, fluid from joints, vitreous fluid, vaginal or urethral secretions, or disaggregated cellular tissues such as hair, skin, synovial tissue, tissue biopsies and nail scrapings.

1400. The method of any of the claims 1292 to 1293, wherein the sample to be analysed is acquired from cell suspensions derived from tissue samples or cell lines.

1401 . The method of claims 1292 to 1293 for use in diagnosis of cancer.

1402. The method of any of the claims 1292 to 1293 for use in monitoring a cancer vaccine response.

1403. The method of any of the claims 1292 to 1293 for use in development of a cancer vaccine.

1404. The method of any of the claims 1292 to 1293, wherein T cells are specific for cancer antigens are detected.

1405. The method of any of the claims 1292 to 1293, wherein the method comprises derivatives of MHC multimers.

1406. The method of any of the claims 1292 to 1293, wherein T cells are specific for cancer associated proteins are detected.

1407. The method of claim 1406, wherein the cancer associated proteins comprises Survivin, Survivin-2B, Livin/ML-IAP, Bcl-2, McI- 1 , BcI-X(L), Mucin-1 , NY-

ESO-1 , Telomerase, CEA, MART-1 , HER-2/neu, bcr-abl, PSA, PSCA, Tyrosinase, p53, hTRT, Leukocyte Proteinase-3, hTRT, gp100, MAGE antigens, GASC, JMJD2C, JARD2 (JMJ), JHDM3a, WT-1 ,CA 9 and Protein kinases, in relation to clinical manifestations such as malignant melanoma, renal carcinoma, breast cancer, lung cancer, cancer of the uterus, cervical cancer, prostatic cancer, pancreatic cancer, brain cancer, head and neck cancer, leukemia, cutaneous lymphoma, hepatic carcinoma, colorectal cancer and bladder cancer.

1408. A method for performing a cancer vaccination of an individual in need thereof, said method comprising the steps of providing a MHC monomer or MHC multimer according to any of the claims 1 to 797, and

administering said MHC multimer to said individual, thereby performing a cancer vaccination of the said individual.

1409. The method according to claim 1408, wherein the MHC multimer is administered alone to said individual.

1410. The method according to claim 1408, wherein the MHC multimer is administered together with one or more adjuvant(s) to said individual.

141 1. The method according to claim 1408, wherein the MHC multimer is administered to said individual more than once such as twice or three times.

1412. The method according to claim 1408, wherein the MHC multimer is administered to said individual cutaneously.

1413. The method according to claim 1408, wherein the MHC multimer is administered to said individual subcutaneously (SC).

1414. The method according to claim 1408, wherein the MHC multimer is administered to said individual by intramuscular (IM) administration.

1415. The method according to claim 1408, wherein the MHC multimer is administered to said individual by intravenous (IV) administration.

1416. The method according to claim 1408, wherein the MHC multimer is administered to said individual by Per-oral (PO) administration.

1417. The method according to claim 1408, wherein the MHC multimer is administered to said individual inter peritoneally.

1418. The method according to claim 1408, wherein the MHC multimer is administered to said individual pulmonally.

1419. The method according to claim 1408, wherein the MHC multimer is administered to said individual vaginally.

1420. The method according to claim 1408, wherein the MHC multimer is administered to said individual rectally.

1421. The method according to claim 1408, wherein the cancer vaccination is a therapeutic vaccination i.e. vaccination "teaching" the immune system to fight an existing infection or disease.

1422. A method for performing a cancer therapeutic treatment of an individual comprising the steps of

Providing the MHC multimer according to claim 1 -797, and

Isolation of T cells specific for said MHC multimer, and

Optionally manipulation of said T cell and

Introduction of said T cells into the same or a different individual to obtain a cancer therapeutic treatment.

1423. A method for minimization of undesired binding of the MHC multimer according to any of the claims 1 to 782.

1424. The method of claim 1423, wherein the method comprises mutagenesis of the MHC multimer.

1425. The method of claim 1424, wherein the method comprises mutagenesis in areas of the MHC multimer responsible for binding to unwanted cells.

1426. The method of claim 1424, wherein the method comprises mutagenesis in the α3-domain of the α-chain of MHC I molecules.

1427. The method of claim 1424, wherein the method comprises mutagenesis in a sub domain in the β2 domain of the β-chain of MHC Il molecules.

1428. The method of claim 1424, wherein the method comprises mutagenesis in areas of MHC multimers that are involved in interactions with T cell surface receptors different from TCR, CD8 and CD4, or that bind surface receptors on B cells, NK cells, Eosiniophils, Neutrophils, Basophiles, Dendritic cells or monocytes.

1429. The method of any of the claims 1424 to 1428, wherein the mutation is a substitution.

1430. The method of any of the claims 1424 to 1428, wherein the mutation is an insertion.

1431 . The method of any of the claims 1424 to 1428, wherein the mutation is a deletion.

1432. The method of any of the claims 1424 to 1431 , wherein the one or more target residues are natural amino acids.

1433. The method of any of the claims 1424 to 1431 , wherein the one or more target residues are non-natural amino acids.

1434. The method of any of the claims 1424 to 1431 , wherein the target residues are a combination of natural and non-natural amino acids.

1435. The method of any of the claims 1424 to 1434, wherein a single amino acid is mutated.

1436. The method of any of the claims 1424 to 1434, wherein more than one amino acid is mutated.

1437. The method for minimization of undesired binding of the MHC multimer according to claim 1423, wherein the method comprises chemical alterations in the MHC multimer.

1438. The method of claim 1437, wherein the method comprises one or more chemical alterations of surface areas of the MHC multimer responsible for binding to unwanted cells.

1439. The method of claim 1437, wherein the method comprises one or more chemical alterations in the oc3-domain of the α-chain of MHC I molecules.

1440. The method of claim 1437, wherein the method comprises one or more chemical alterations in a sub domain in the β2 domain of the β-chain of MHC Il molecules.

1441 . The method of claim 1437, wherein the method comprises one or more chemical alterations of surface areas of MHC multimers that are involved in interactions with T cell surface receptors different from TCR, CD8 and CD4, or that bind surface receptors on B cells, NK cells, Eosiniophils, Neutrophils, Basophiles, Dendritic cells or monocytes.

1442. The method of any of the claims 1437 to 1441 , wherein a single amino acid is altered.

1443. The method of any of the claims 1437 to 1441 , wherein more than one amino acid is altered.

1444. The method of claim 1423, wherein the method comprises the addition of one or more components of a MHC multimer, predicted to be responsible for the undesired binding.

1445. The method of claim 1444, wherein the added component is one or more MHC multimers that contain nonsense peptides.

1446. The method of claim 1444, wherein the added component is one or more soluble MHC multimers not coupled to a multimerization domain, and with or without peptide bound in the peptide binding cleft.

1447. The method of claim 1444, wherein the added component is individual components of the MHC multimer.

1448. The method of claim 1447, wherein one or more individual components of the MHC multimer is selected from the group consisting of I α-chain, subunits of MHC I α-chain, beta2microglobulin, subunits of beta2microglobulin , α/β-chain of MHC II, subunits of α/β-chain of MHC II.

1449. The method of claim 1447, wherein the one or more individual subunits are folded.

1450. The method of claim 1447, wherein the one or more individual subunits are un-folded.

1451 . The method of claim 1447, wherein the individual subunits are folded and un-folded.

1452. The method of claim 1447, wherein the one or more individual subunits are attached to one or more multimerization domains identical or different from the one used in the MHC multimer employed in the analysis.

1453. The method of claim 1447, wherein the one or more individual subunits are one or more multimerization domain similar or identical to the multimerization domain used in the MHC multimer or individual components of the multimerization domain.

1454. The method of any of the claims 1447 to 1453, wherein the added component is not labelled.

1455. The method of any of the claims 1447 to 1453, wherein the added component is labelled with a label different from the label of the MHC multimer used for analysis.

1456. The method of claim 1423, wherein one or more reagents able to identify specific cell types are included.

1457. The method of claim 1456, wherein the one or more reagents comprises gating reagents for use in flow cytometry experiments.

1458. The method of claim 1457, wherein the flow cytometry experiment comprises removal of signals from MHC multimer stained cells not expressing the specific TCR by introducing an exclusion gate.

1459. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD3.

1460. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD8.

1461. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD3 and CD8.

1462. The method of claim 1461 , wherein the CD3 and CD8 are labelled with distinct labels such as fluorochromes.

1463. The method of claim 1461 , wherein the CD3 and CD8 are labelled with the same labels such as fluorochromes.

1464. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD4.

1465. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD3 and CD4.

1466. The method of claim 1465, wherein the CD3 and CD4 are labelled with distinct labels such as fluorochromes.

1467. The method of claim 1465, wherein the CD3 and CD4 are labelled with the same labels such as fluorochromes.

1468. The method of claim 1457, wherein the one or more reagents comprises fluorescent antibodies directed against specific surface markers.

1469. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD2.

1470. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD27 and/or CD28.

1471. The method of claim 1457, wherein the one or more gating reagents comprises anti-Foxp3.

1472. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD45RA.

1473. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD45RO.

1474. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CD62L.

1475. The method of claim 1457, wherein the one or more gating reagents comprises reagents binding to CCR7.

1476. The method of any of the claims 1456 to 1475, wherein the reagent identifies specific cell types by selection.

1477. The method of any of the claims 1456 to 1475, wherein the reagent identifies specific cell types by exclusion.

1478. The method of claim 1477, wherein the reagent identifies CD45 expressed on red blood cells.

1479. The method of claim 1477, wherein the reagent identifies CD19 expressed on B cells.

1480. The method of claim 1477, wherein the reagent identifies CD56 expressed on NK cells.

1481 . The method of claim 1477, wherein the reagent identifies CD4 expressed on T helper cells.

1482. The method of claim 1477, wherein the reagent identifies CD8 expressed on cytotoxic T cells.

1483. The method of claim 1477, wherein the reagent identifies CD14 expressed on monocytes.

1484. The method of claim 1477, wherein the reagent identifies CD15 expressed on granulocytes.

1485. The method of claim 1477, wherein the reagent identifies CD15 expressed on monocytes.

1486. The method of claim 1423 further comprising co-staining with reagents according to any of the claims 1 to 782.

1487. A method for performing a control experiment comprising the step of counting of particles comprising the MHC multimer according to any of the claims 1 to

797.

1488. A method for performing a control experiment comprising the step of sorting of particles comprising the MHC multimer according to any of the claims 1 to 797.

1489. A method for performing a control experiment comprising the step of performing flow cytometry analysis of particles comprising the MHC multimer according to any of the claims 1 to 797.

1490. A method for performing a control experiment comprising the step of performing a immunohistochemistry analysis comprising the MHC multimer according to any of the claims 1 to 797.

1491 . A method for performing a control experiment comprising the step of performing a immunocytochemistry analysis comprising the MHC multimer according to any of the claims 1 to 797.

1492. A method for performing a control experiment comprising the step of performing an ELISA analysis comprising the MHC multimer according to any of the claims 1 to 797.

1493. The method of any of the claims 1487 to 1492, further comprising a positive control (recognizing the MHC multimers comprising correctly folded MHC).

1494. The method of claim 1493, wherein the positive control comprises one or more beads with immobilized TCR's.

1495. The method of claim 1493, wherein the positive control comprises one or more "solid support" scaffolds with immobilized TCR's.

1496. The method of claim 1493, wherein the positive control comprises a solid surface with immobilized TCR's.

1497. The method of claim 1496, wherein the solid surface is a microtiter plate.

1498. The method of claim 1493, wherein the positive control comprises one or more micelles with immobilized TCR's.

1499. The method of claim 1493, wherein the positive control comprises one or more liposomes with immobilized TCR's.

1500. The method of claim 1493, wherein the positive control comprises soluble TCR's.

1501 . The method of any of the claims 1494 to 1500, wherein the TCR's are full-length.

1502. The method of any of the claims 1494 to 1500, wherein the TCR's are truncated.

1503. The method of any of the claims 1494 to 1500, wherein the TCR's are full-length or truncated.

1504. The method of any of the claims 1494 to 1500, wherein the TCR's only comprises the extracellular domains.

1505. The method of any of the claims 1494 to 1500, wherein the TCR's are recombinant.

1506. The method of any of the claims 1494 to 1500, wherein the TCR's are chemically modified.

1507. The method of any of the claims 1494 to 1500, wherein the TCR's are enzymatically modified.

1508. The method of claim 1493, wherein the positive control comprises one or more beads with immobilized aptamers.

1509. The method of claim 1493, wherein the positive control comprises one or more "solid support" scaffolds with immobilized aptamers.

1510. The method of claim 1493, wherein the positive control comprises a solid surface with immobilized aptamers.

151 1 . The method of claim 1510, wherein the solid surface is a microtiter plate.

1512. The method of claim 1493, wherein the positive control comprises one or more micelles with immobilized aptamers.

1513. The method of claim 1493, wherein the positive control comprises one or more liposomes with immobilized aptamers.

1514. The method of claim 1493, wherein the positive control comprises one or more soluble aptamers.

1515. The method of claim 1493, wherein the positive control comprises one or more beads with immobilized antibody specific for the MCH mutimer.

1516. The method of claim 1493, wherein the positive control comprises one or more "solid support" scaffolds with immobilized antibody specific for the MCH mutimer.

1517. The method of claim 1493, wherein the positive control comprises a solid surface with immobilized antibody specific for the MCH mutimer.

1518. The method of claim 1517, wherein the solid surface is a microtiter plate.

1519. The method of claim 1493, wherein the positive control comprises one or more micelles with immobilized antibody specific for the MCH mutimer.

1520. The method of claim 1493, wherein the positive control comprises one or more liposomes with immobilized antibody specific for the MCH mutimer.

1521. The method of claim 1493, wherein the positive control comprises one or more soluble antibody specific for the MCH mutimer.

1522. The method of claim 1493, wherein the positive control comprises one or more beads with immobilized molecule that recognize correctly folded MHC-peptide complexes.

1523. The method of claim 1493, wherein the positive control comprises one or more "solid support" scaffolds with immobilized molecule that recognize correctly folded MHC-peptide complexes.

1524. The method of claim 1493, wherein the positive control comprises a solid surface with immobilized molecule that recognize correctly folded MHC-peptide complexes.

1525. The method of claim 1524, wherein the solid surface is a microtiter plate.

1526. The method of claim 1493, wherein the positive control comprises one or more micelles with immobilized molecule that recognize correctly folded MHC- peptide complexes.

1527. The method of claim 1493, wherein the positive control comprises one or more liposomes with immobilized molecule that recognize correctly folded MHC- peptide complexes.

1528. The method of claim 1493, wherein the positive control comprises one or more soluble molecule that recognize correctly folded MHC-peptide complexes.

1529. The method of claim 1493, wherein the positive control comprises one or more cell lines that display MHC multimer-binding molecules that recognize the MHC-peptide complexes.

1530. The method of claim 1529, wherein the cell line is a T-cell line.

1531. The method of claim 1529, wherein the MHC multimer-binding molecules are TCR's.

1532. The method of claim 1493, wherein the positive control comprises cells from blood samples that carry receptor molecules specific for the MHC multimer.

1533. The method of claim 1493, wherein the positive control comprises cells from preparations of purified lymphocytes (HPBMCs) that carry receptor molecules specific for the MHC multimer.

1534. The method of claim 1493, wherein the positive control comprises cells from bodily fluids that carry receptor molecules specific for the MHC multimer.

1535. The method of claim 1493, wherein the positive control comprises cells from bodily fluids that carry receptor molecules specific for the MHC multimer.

1536. The method of claim 1493, wherein the said method comprises density- gradient centrifugation (e.g. in CsCI).

1537. The method of claim 1493, wherein the said method comprises size exclusion chromatography.

1538. The method of claim 1493, wherein the said method comprises PAGE.

1539. The method of claim 1493, wherein the said method comprises chromatography.

1540. The method of any of the claims 1487 to 1492, wherein the MHC multimer binding is visualized with a labelled secondary component that binds the MHC multimer

1541 . The method of claim 1540, wherein the label of the secondary component is selected from the group consisting of fluorophores, chromophores, chemiluminescent labels, bioluminescent labels, radioactive labels, enzyme labels.

1542. The method of any of the claims 1540 and 1541 , wherein the MHC multimer binding is visualized by binding of a labelled compound specific for the secondary component that binds the MHC multimer selected from the group of TCR's, aptamers, antibodies, streptavidin or other MHC-peptide complex binding molecules.

1543. The method of claim 1541 , wherein the label of the compound specific for the secondary component is selected from the group consisting of fluorophores, chromophores, chemiluminescent labels, bioluminescent labels, radioactive labels, enzyme labels.

1544. The method of any of the claims 1487 to 1543, wherein a negative control is included

1545. The method of claim 1544, wherein the method comprises MHC multimers carrying nonsense peptides

1546. The method of claim 1544, wherein the method comprises MHC multimers carrying nonsense chemically modified peptides

1547. The method of claim 1544, wherein the method comprises MHC multimers carrying naturally occurring peptides different from the peptide used for analysis of specific T cells in the sample

1548. The method of claim 1544, wherein the method comprises empty MHC multimers

1549. The method of claim 1544, wherein the method comprises MHC heavy chain or MHC multimers comprising MHC heavy chain and not the MHC light chain

1550. The method of claim 1549, wherein the MHC heavy chain is MHC I

1551 . The method of claim 1549, wherein the MHC heavy chain is MHC Il

1552. The method of claims 1550 and 1551 , wherein the MHC heavy chain is truncated

1553. The method of claims 1550 and 1551 , wherein the MHC heavy chain is full-length.

1554. The method of claims 1550 and 1551 , wherein the MHC heavy chain is folded.

1555. The method of claims 1550 and 1551 , wherein the MHC heavy chain is un-folded.

1556. The method of claim 1550, wherein the MHC I heavy chain is the MHC I alpha chain comprising the α3 domain that binds CD8 molecules on cytotoxic T cells.

1557. The method of claim 1551 , wherein the MHC Il heavy chain is the MHC Il β chain comprising the β2 domain that binds CD4 on the surface of helper T cells.

1558. The method of claim 1557, wherein the method comprises Beta2microglobulin.

1559. The method of claim 1544, wherein the method comprises subunits of beta2microglobulin.

1560. The method of claim 1544, wherein the method comprises MHC multimers comprising Beta2microglobulin.

1561 . The method of claim 1544, wherein the method comprises MHC multimers comprising subunits of beta2microglobulin.

1562. The method of claims 1558 to 1562, wherein the Beta2microglobulin is un-folded.

1563. The method of claims 1558 to 1562, wherein the Beta2microglobulin is folded.

1564. The method of claims 1558 to 1562, wherein the subunit of Beta2microglobulin is un-folded.

1565. The method of claims 1558 to 1562, wherein the subunit of Beta2microglobulin is folded.

1566. The method of claim 1544, wherein the method comprises MHC-like complexes.

1567. The method of claim 1566, wherein the MHC-like complexes are folded.

1568. The method of claim 1566, wherein the MHC-like complexes are unfolded.

1569. The method of claim 1566, wherein the MHC-like complexes are CD1 molecules.

1570. The method of claim 1567, wherein the method comprises multimerization domains without MHC or MHC-like molecules.

1571. The method of claim 1544, wherein the method comprises one or more multimerization domains selected from the group consisting of dextran, streptavidin,

IgG, coiled-coil-domain, liposomes.

1572. The method of claim 1544, wherein the method comprises one or more labels selected from the group FITC, PE, APC, pacific blue, cascade yellow.

1573. The method of any of the claims 1487 to 1492, wherein a negative and a positive control is included.

1574. The method according to any of the claims 1093 to 1573, wherein the method comprises use of one or more MHC monomer and/or one or more MHC multimer according to any of the claims 1 to 797, wherein said one or more MHC monomers and/or MHC multimers are part of an array or other kind of library of MHC monomers or MHC multimers.

1575. The method according to claim 1574, wherein said array or library of MHC monomers or MHC multimers comprises at least 10, such as at least 100, such as at least 1000, such as at least 10.000, such as at least 100.000, such as at least 1.000.0000, such as at least 10.000.000 different antigenic peptides.

1576. The method according to claim 1575, wherein the antigenic peptides are made by organic synthesis.

1577. The method according to claim 1575, wherein the antigenic peptides comprise all possible 8'-, 9'-, 10'-, and 1 1 '-mers of one antigen.

1578. The method according to claim 1575, wherein the antigenic peptides comprise all possible 13'-, 14'-, 15'-, and 16'-mers of one antigen.

1579. The method of claims 1575-1579, where the antigen is a CMV antigen such as an antigen selected from the group consisting of YP 081531 .1 tegument protein pp65 (SEQ ID NO 1 ) and YP_081562.1 regulatory protein IE1 (SEQ ID NO 4402).

1580. The MHC monomer or MHC multimer according to any of the claims 1 to

797, wherein said MHC monomer or MHC multimer comprises one of the possible 8'-, 9'-, 10'-, 1 1 '-,13'-, 14'-, 15'-, or 16'-mers of an antigen.

1581 . The MHC monomer or MHC multimer according to claim 1580, wherein the antigen is a CMV antigen such as an antigen selected from the group consisting of

YP_081531.1 tegument protein pp65 (SEQ ID NO 1 ) and YP_081562.1 regulatory protein IE1 (SEQ ID NO 4402).

1582. The composition according to claim 1090 and 1091 , wherein the composition comprises at least 10 different antigenic peptides, such as at least 100, such as at least 1000, such as at least 10.000, such as at least 100.000, such as at least 1 .000.0000, such as at least 10.000.000 different antigenic peptides.

1583. The composition according to claim 1090 and 1091 , wherein the composition comprises least 10 different antigenic peptides derived from one antigen, such as at least 100, such as at least 1000, such as at least 10.000, such as at least 100.000, such as at least 1.000.0000, such as at least 10.000.000 different antigenic peptides derived from one antigen.

1584. The composition according to claim 1583, wherein the antigenic peptides are made by organic synthesis on solid support, and wherein a partial cleavage of the linker that connects the growing antigenic peptide to the solid support is performed before, during or after each additional amino acid that is coupled to the growing antigenic peptide.

1585. A MHC monomer or MHC multimer, wherein the MHC monomer or MHC multimer is comprised within the composition of claim 1584.

1586. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain.

1587. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain combined with a MHC I beta2microglobulin chain.

1588. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain in complex with a MHC I beta2microglobulin chain.

1589. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain combined with MHC I beta2microglobulin chain through a linker.

1590. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain covalently linked to a MHC I beta2microglobulin chain through a linker.

1591. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain combined with an antigenic peptide.

1592. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain in complex with an antigenic peptide.

1593. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain combined with an antigenic peptide through a linker.

1594. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain covalently linked with an antigenic peptide through a linker.

1595. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain/MHC I beta2microglobulin dimer combined with an antigenic peptide.

1596. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain/MHC I beta2microglobulin dimer in complex with an antigenic peptide.

1597. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain/MHC I beta2microglobulin dimer combined with an antigenic peptide through a linker to the heavy chain or beta2microglobulin.

1598. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain/MHC I beta2microglobulin dimer covalently linked to an antigenic peptide through a linker to the heavy chain or beta2microglobulin.

1599. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC I heavy chain and/or beta2microglobulin-chain where the heavy chain or beta2microglobulin-chain is at least 99%, such as at least 98%, such as at least 95%, such as at least 90%, such as at least 80%, such as at least 70%, such as at least 60%, such as at least 50%, such as at least 40%, such as at least 30% identical to wild type MHC I heavy chain or beta2microglobulin.

1600. The MHC monomer or MHC multimer of any of the preceding claims where the MHC I molecule chains differ from wild type MHC I molecule chains by substitution of single or cohorts of native amino acids. o

1601. The MHC monomer or MHC multimer of any of the preceding claims where the MHC I molecule chains differ from wild type MHC I molecule chains by inserts or deletions.

1602. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC Il alpha-chain and a MHC Il beta-chain, i.e. is a MHC Il alpha/beta- dimer.

1603. The MHC monomer or MHC multimer of any of the preceding claims comprising an MHC Il alpha/beta dimer with an antigenic peptide.

1604. The MHC monomer or MHC multimer of any of the preceding claims comprising an MHC Il alpha/beta dimer in complex with an antigenic peptide.

1605. The MHC monomer or MHC multimer of any of the preceding claims comprising an MHC Il alpha/beta dimer combined with an antigenic peptide through a linker to the MHC Il alpha or MHC Il beta chain.

1606. The MHC monomer or MHC multimer of any of the preceding claims comprising an MHC Il alpha/beta dimer covalently linked to an antigenic peptide through a flexible linker to the MHC Il alpha or MHC Il beta chain.

1607. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC Il alpha/beta dimer combined through an interaction by affinity tags.

1608. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC Il alpha/beta dimer held together through an interaction by affinity tags.

1609. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC Il alpha/beta dimer combined through an interaction by affinity tags and further combined with an antigenic peptide through a linker to the MHC Il alpha or MHC Il beta chain. T"

1610. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC Il alpha/beta dimer held together through an interaction by affinity tags and further covalently linked to an antigenic peptide through a flexible linker to the MHC Il alpha or MHC Il beta chain.

161 1. The MHC monomer or MHC multimer of any of the preceding claims comprising a MHC Il alpha- or beta-chain where the alpha- and/or beta-chain are at least 99%, such as at least 98%, such as at least 95%, such as at least 90%, such as at least 80%, such as at least 70%, such as at least 60%, such as at least 50% identical to wild type MHC Il alpha- or beta-chains.

1612. The MHC monomer or MHC multimer of any of the preceding claims where the MHC Il molecule chains differ from wild type MHC Il by substitution of single or cohorts of native amino acids.

1613. The MHC monomer or MHC multimer of any of the preceding claims where the MHC Il molecule chains differ from wild type MHC Il molecule chains by inserts or deletions.

1614. The method of any of the preceding claims wherein the MHC monomer or

MHC multimer comprises a MHC I heavy chain.

1615. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain combined with a MHC I beta2microglobulin chain.

1616. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain in complex with a MHC I beta2microglobulin chain.

1617. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain combined with MHC I beta2microglobulin chain through a linker.

1618. The method of any of the preceding claims wherein the MHC monomer or

MHC multimer comprises a MHC I heavy chain covalently linked to a MHC I beta2microglobulin chain through a linker.

1619. The method of any of the preceding claims wherein the MHC monomer or

MHC multimer comprises a MHC I heavy chain combined with an antigenic peptide.

1620. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain in complex with an antigenic peptide.

1621. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain combined with an antigenic peptide through a linker.

1622. The method of any of the preceding claims wherein the MHC monomer or

MHC multimer comprises a MHC I heavy chain covalently linked with an antigenic peptide through a linker.

1623. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain/MHC I beta2microglobulin dimer combined with an antigenic peptide.

1624. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain/MHC I beta2microglobulin dimer in complex with an antigenic peptide.

1625. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain/MHC I beta2microglobulin dimer combined with an antigenic peptide through a linker to the heavy chain or beta2microglobulin.

1626. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC I heavy chain/MHC I beta2microglobulin dimer covalently linked to an antigenic peptide through a linker to the heavy chain or beta2microglobulin.

1627. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises MHC Il molecule chains that differ from wild type MHC I molecule chains by substitution of single or cohorts of native amino acids.

1628. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises MHC Il molecule chains that differ from wild type MHC I molecule chains by inserts or deletions.

1629. The method of any of the preceding claims wherein the MHC monomer or

MHC multimer comprises MHC Il alpha-chain and a MHC Il beta-chain, i.e. is a MHC Il alpha/beta-dimer.

1630. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises an MHC Il alpha/beta dimer with an antigenic peptide.

1631. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises an MHC Il alpha/beta dimer in complex with an antigenic peptide.

1632. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises an MHC Il alpha/beta dimer combined with an antigenic peptide through a linker to the MHC Il alpha or MHC Il beta chain.

1633. The method of any of the preceding claims wherein the MHC monomer or

MHC multimer comprises an MHC Il alpha/beta dimer covalently linked to an antigenic peptide through a linker to the MHC Il alpha or MHC Il beta chain.

1634. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC Il alpha/beta dimer combined through an interaction by affinity tags.

1635. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC Il alpha/beta dimer held together through an interaction by affinity tags.

1636. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC Il alpha/beta dimer combined through an interaction by affinity tags and further combined with an antigenic peptide through a linker to the MHC Il alpha or MHC Il beta chain.

1637. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises a MHC Il alpha/beta dimer held together through an interaction by affinity tags and further covalently linked to an antigenic peptide through a linker to the MHC Il alpha or MHC Il beta chain.

1638. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises MHC Il molecule chains that differ from wild type MHC Il by substitution of single or cohorts of native amino acids.

1639. The method of any of the preceding claims wherein the MHC monomer or MHC multimer comprises MHC Il molecule chains that differ from wild type MHC Il molecule chains by inserts or deletions.

1640. The method according to any of claims 1093-1276 or 1292-1407, wherein the method comprises the step of measurement of the number of T cells.

1641 . The method according to any of claims 1093-1276 or 1292-1407, wherein the method does not comprise the step of measurement of the activation of T cells.

1642. The method according to any of claims 1093-1276 or 1292-1407, wherein the method comprises use of a binding assay.

1643. The method according to any of claims 1093-1276 or 1292-1407, wherein the method does not comprise use of a functional assay.

1644. The method according to any of claims 1093-1276 or 1292-1407, wherein the method does not comprise a step of priming of antigen presenting cells.

1645. The method according to any of claims 1093-1276 or 1292-1407, wherein the method comprises a step to limit and/or prevent priming of antigen presenting cells.

1646. The MHC multimer according to any of claims 1 -797 and 1585-1612, wherein the MHC multimer comprises one or more chemically biotinylated MHC dextramers.

1647. The MHC multimer according to any of claims 1 -797 and 1585-1612, wherein the MHC multimer comprises one or more chemically biotinylated MHC dextramers comprising one or more peptides derived from one or more CMV antigens.

1648. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen pp50.

1649. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen pp28.

1650. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen pp65.

1651. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen pp150.

1652. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen pp71.

1653. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen gH.

1654. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen gB.

1655. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen IE-1.

1656. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen IE-2.

1657. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen US3.

1658. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen US2.

1659. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen US6.

1660. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen US1 1.

1661 . The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides are derived from the CMV antigen UL18.

1662. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence

VTEHDTLLY (SEQ ID NO 9689).

1663. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence IPSINVHHY (SEQ ID NO 9697).

1664. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence NLVPMVATV (SEQ ID NO 9672).

1665. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence QYDPVAALF (SEQ ID NO 9683).

1666. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence VYALPLKML (SEQ ID NO 9684).

1667. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence RPHERNGFTVL (SEQ ID NO 9679).

1668. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence

TPRVTGGGAM (SEQ ID NO 9678).

1669. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence ELRRKMMYM (SEQ ID NO 5100).

1670. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence KLGGALQAK (SEQ ID NO 5085).

1671 . The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence VLEETSVML.

1672. The MHC multimer according to any of claims 1 -797 and 1647, wherein the one or more peptides comprises at least one peptide with the sequence QIKVRVDMV (SEQ ID NO 4989).