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WO2010020371A1 - Microscope et procédé d’examen au microscope - Google Patents

Microscope et procédé d’examen au microscope Download PDF

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Publication number
WO2010020371A1
WO2010020371A1 PCT/EP2009/005870 EP2009005870W WO2010020371A1 WO 2010020371 A1 WO2010020371 A1 WO 2010020371A1 EP 2009005870 W EP2009005870 W EP 2009005870W WO 2010020371 A1 WO2010020371 A1 WO 2010020371A1
Authority
WO
WIPO (PCT)
Prior art keywords
unit
control module
sample
focus
trigger signal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2009/005870
Other languages
German (de)
English (en)
Inventor
Steffen Leidenbach
Stefan Steinborn
Tobias Klinge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carl Zeiss Microscopy GmbH
Original Assignee
Carl Zeiss MicroImaging GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss MicroImaging GmbH filed Critical Carl Zeiss MicroImaging GmbH
Publication of WO2010020371A1 publication Critical patent/WO2010020371A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

Definitions

  • the present invention relates to a microscope according to the preamble of claim 1.
  • an image series of the sample is to be taken in different focal planes in order to extract from these data e.g.
  • the desired focus position is set by means of the movement unit, after waiting for a predetermined period of time after setting the focus position, so that calms the system, and then created the recording by means of the recording unit.
  • the object is achieved in a microscope of the type mentioned above in that the control module controls the movement unit to start a focus travel, wherein the movement unit continuously changes the distance between the sample stage and the imaging optics, and the control module in each case upon reaching a predetermined focus position Trigger signal is generated and applied to the recording unit, which receives the sample in response to the trigger signal during focus travel.
  • the necessary images can be performed during the focus travel, so that a stopping of the sample stage and / or the imaging optics is no longer necessary, the recordings are always made at the right time due to the trigger signal applied to the recording unit. This ensures that the recording can always be made when one of the predetermined focus hunts is reached.
  • connection between the control module and the recording unit is preferably not a bus system in which usually can not be accurately determined due to unpredictable latency when the signal is actually applied to the recording unit.
  • the movement unit may perform the focus travel such that there is a constant rate of change in the distance. This is very easy to realize.
  • the movement unit can be designed so that only the imaging optics or the sample table is moved in the direction of picking.
  • the control module may include a memory in which the predetermined focus positions are stored as discrete position information.
  • the control module may include a focus controller that communicates directly with the motion unit and has the memory for the predetermined focal positions. This makes it possible to generate the trigger signal quickly enough during the movement and to apply it to the recording unit.
  • control module may include a trigger card that is directly connected to the capture unit and applies the trigger signal to the capture unit. This ensures that the triggering signal is always applied to the recording unit at the same speed.
  • the control module can be designed so that the polarity and / or the pulse duration of the trigger signal can be changed.
  • the polarity and / or the pulse duration of the trigger signal can be adjusted by means of the control module.
  • an adaptation to different trigger receiver of the recording unit can be easily performed.
  • the object is further achieved in a microscope of the type mentioned above in that the control module, the movement unit to start a focus drive, in which the Moving unit continuously changes the distance between the sample table and the imaging optics, and controls the recording unit to start a series of photographs, in which the recording unit successively generates several recordings of the sample, the recording unit generates a trigger signal with each recording and applies it to the control module, that detects and stores the current focus position in response to the trigger signal
  • the moving unit may make the focus travel such that there is a constant pitch changing speed.
  • the pitch changing speed is not constant
  • the control module may include a focus controller that communicates directly with the motion unit and has memory for the detected focus locations
  • control module may include a trigger card which is directly connected to the recording unit and to which the recording unit applies the trigger signal
  • a predetermined focus position is understood to mean, in particular, a predetermined optical path length of the microscope image.
  • a predetermined focus position does not have to be the best focus position, but rather the focus position desired by the user of the microscope
  • the microscope can be configured as a reflected-light or transmitted-light microscope.
  • the microscope can furthermore be designed as a confocal microscope, as a laser scanning microscope and / or as a fluorescence microscope.
  • the microscope can have a lighting unit and / or an input unit via which the microscope is operated can be
  • the microscope can have further parts known to the person skilled in the art which are necessary for the operation of the microscope
  • a microscopy method for a microscope of the aforementioned type in which the control module controls the movement unit to start a focus run, in which the movement unit continuously changes the distance between the sample stage and the imaging optics, and the control module in each case upon reaching predetermined focus position generates a trigger signal and applies to the recording unit, which receives in response to the trigger signal, the sample during the focus travel
  • a microscopy method for a microscope of the type mentioned in which the control module controls the movement unit to start a focus run, in which the moving unit continuously changes the distance between the sample table and the imaging optics, and the recording unit to start a series of photographs, wherein the recording unit temporally successively produces a plurality of recordings of the sample, wherein the recording unit generates a trigger signal at each recording and applies it to the control module, which detects and stores the current focus position in response to the trigger signal
  • the determined and stored focus positions can be used in the first microscopy method described above in order to start a renewed focus run and to take pictures of the sample again at the same focal positions
  • FIG. 2 is a flowchart for explaining an operating mode of the microscope of FIG. 1;
  • FIG. 3 shows a flowchart for explaining a further operating mode of the microscope of FIG.
  • the microscope 1 comprises a sample table 2 which carries a sample 3, a receiving unit 4, a movement unit 5 and a control module 6
  • the recording unit 4 contains an imaging optics 7 and a camera 8, which can receive an enlarged image of the sample 2 generated by means of the imaging optics 7.
  • the movement unit 5 is designed so that it can change the distance between the imaging optics 7 and the sample stage 2 and thus the sample 3. This allows different focal positions or imaging positions to be set, so that different levels can be recorded within the sample 3.
  • the movement unit 5 is preferably designed so that it moves only the imaging optics 7 or the sample table 2 in the imaging direction for changing the distance.
  • the control module 6 comprises, as the schematic representation in Fig. 1 can be seen, a focus controller 9, which, as indicated by the double arrow P1, the movement unit 5 drives and also receives from the movement unit 5, a signal that is currently available Distance between sample table 2 and imaging optics 7 corresponds.
  • control module contains a trigger card 10, a main controller 11 and a computer 12.
  • the trigger card 10 communicates with the focus controller 9 (double arrow P2) and is connected to the camera 8 via two lines L1 and L2.
  • a trigger signal can be transmitted to the camera 8 via the line L1. Via the line L2, a trigger signal from the camera 8 to the trigger card 10 can be transmitted.
  • the focus controller 9 is connected via a bus connection P3 to the main controller 11, which in turn communicates bidirectionally with the computer 12 via the connection P4.
  • the connection P4 may be, for example, a bus connection (e.g., USB connection) or a connection via a serial interface.
  • the computer 12 is connected to the camera 8 via a further bus connection P5.
  • the bus connection P5 can be designed, for example, as an IEEE1394 interface.
  • an image series in different focal planes can be recorded from the sample 3 as follows.
  • a position list of the focus or image planes in which a recording of the sample 3 is to be made in each case is stored in a memory 13 of the focus controller 9.
  • the position list can be generated for example by means of the computer 12 and then be stored on the main controller 1 1 in the focus controller 9 (step S1 in Fig. 2).
  • the movement unit 5 is controlled by the computer 12 via the main and the focus controller 11, 9 so that a focus drive is started, in which the distance between the sample 2 and the imaging optics 7 is changed continuously. For example, the distance can be reduced.
  • step S3 the focus controller 9 continuously compares the actual position supplied by the movement unit 5 with the positions of the stored position list. This comparison is repeated until a match is found.
  • the focus controller 9 notifies the trigger card 10, which generates a trigger signal and applies it to the camera 8 via the line L1.
  • the camera 8 picks up the sample in response to the trigger signal and transmits the generated image signal to the computer 12 via the connection P5. All this is done during the continuous change in distance between sample 3 and imaging optics 7.
  • the recording is generated so fast that despite the change in distance, the desired focal plane is displayed (step S4).
  • step S5 After completion of the recording, it is checked in step S5 whether all the positions indicated in the position list have already been reached. If this is not the case, the process proceeds to step S3.
  • step S6 in which the focus travel is stopped.
  • the images of the image series thus obtained can be further processed.
  • a 3D model can be generated from the images of the image series by means of known software algorithms or the depth of field of the image can be increased.
  • An advantage of this method is that the image series can be recorded in a very short time. For example, it is also possible to record samples containing changing cells or very light-sensitive material.
  • the position list can be generated by means of the computer 12, for example, so that all successive, discrete positions are individually specified. However, it is also possible to have a starting point and the number of positions at a given equidistant To adjust the distance. Furthermore, any function can be specified, from which the discrete focus positions can be derived according to certain rules.
  • step S10 similar to step S2, the focus travel is started.
  • step S11 a continuous image acquisition is started by means of the computer 12.
  • the continuous image recording for example, it is determined that n images are recorded with a certain time interval.
  • step S12 the trigger card 10 checks whether a trigger signal is present from the camera 8 via the line L2.
  • the camera 8 generates a trigger signal whenever it picks up the sample 3.
  • the trigger card 10 informs the focus controller 9 which stores the current focus position in its memory 13 (step S13).
  • step S14 it is checked whether the image recording is still running. If this is the case, the process proceeds to step S12.
  • the image pickup is completed (i.e., when all the images have been taken)
  • the focus travel is stopped in step S15. Furthermore, the position list stored in the memory of the focus controller 9 is transmitted to the computer 12 via the main controller 11.
  • the image data or the images of the camera 8 can then be processed together with the position data, for example to produce a 3D model of the sample 3.
  • step S15 it is possible to stop the focus travel in step S15, restore the original distance between sample 3 and imaging optics 7, and then perform steps S2-S6 of the flow of FIG. 2 with the positions still stored in the focus controller. This retracts the focus distance and retakes it from the same focus levels.

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  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Physics & Mathematics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Microscoopes, Condenser (AREA)
  • Automatic Focus Adjustment (AREA)

Abstract

L'invention concerne un microscope équipé d'une platine porte-échantillon (2) servant de support à un échantillon (3), d'une unité de prise de vues (4) comportant une optique d'imagerie (7) et destinée à réaliser une image de l'échantillon (3), d'une unité de déplacement (5) permettant de faire varier la distance entre la platine porte-échantillon (2) et l'optique d'imagerie (7) afin de régler la position focale, ainsi que d'un module de commande (6) destiné à commander l'unité de prise de vues (4) et l'unité de déplacement (5). Le module de commande (6) commande l'unité de déplacement (5) pour démarrer un ajustement focal au cours duquel l'unité de déplacement (5) fait varier continuellement la distance entre la platine porte-échantillon (2) et l'optique d'imagerie (7). A chaque fois qu'une position focale prédéterminée est atteinte, le module de commande (6) génère un signal de déclenchement et l'applique à l'unité de prise de vues (4) qui, en réaction au signal de déclenchement, photographie l'échantillon (3) durant l'ajustement focal.
PCT/EP2009/005870 2008-08-19 2009-08-13 Microscope et procédé d’examen au microscope Ceased WO2010020371A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008038359.7 2008-08-19
DE102008038359A DE102008038359A1 (de) 2008-08-19 2008-08-19 Mikroskop und Mikroskopierverfahren

Publications (1)

Publication Number Publication Date
WO2010020371A1 true WO2010020371A1 (fr) 2010-02-25

Family

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Application Number Title Priority Date Filing Date
PCT/EP2009/005870 Ceased WO2010020371A1 (fr) 2008-08-19 2009-08-13 Microscope et procédé d’examen au microscope

Country Status (2)

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DE (1) DE102008038359A1 (fr)
WO (1) WO2010020371A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9715095B2 (en) 2011-10-11 2017-07-25 Carl Zeiss Microscopy Gmbh Microscope and method for SPIM microscopy

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012217745B4 (de) * 2012-09-28 2020-02-06 Carl Zeiss Microscopy Gmbh Verfahren und Mikroskop zur Aufnahme eines z-Bildstapels aus Bildern von vorbestimmten z-Ebenen eines Objektes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6344930B1 (en) * 1999-03-03 2002-02-05 Denso Corporation Total-focus imaging apparatus using a variable-focus lens
WO2003012518A2 (fr) * 2001-07-31 2003-02-13 Interscope Technologies, Inc. Systeme servant a creer des images microscopiques de montage numerique
US6724419B1 (en) * 1999-08-13 2004-04-20 Universal Imaging Corporation System and method for acquiring images at maximum acquisition rate while asynchronously sequencing microscope devices
US20050089208A1 (en) * 2003-07-22 2005-04-28 Rui-Tao Dong System and method for generating digital images of a microscope slide
EP1895347A1 (fr) * 2006-09-01 2008-03-05 Universität Zürich Microscope à balayage et procédé de fonctionnement d'un microscope à balayage

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19744246A1 (de) * 1997-10-07 1999-04-29 Hajo Prof Dr Suhr Verfahren und Vorrichtung zur Videomikroskopie disperser Partikelverteilungen
JP2003233014A (ja) * 2002-02-12 2003-08-22 Pentax Corp 双眼鏡
DE102006012364A1 (de) * 2005-03-31 2006-10-05 Alstom Technology Ltd. Verfahren zum optischen Messen von Lagezuständen von Rotorkomponenten
US7417213B2 (en) * 2005-06-22 2008-08-26 Tripath Imaging, Inc. Apparatus and method for rapid microscopic image focusing having a movable objective
DE202008004271U1 (de) * 2008-03-28 2008-05-21 Leica Microsystems (Schweiz) Ag Mikroskop umfassend wenigstens zwei Komponenten

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6344930B1 (en) * 1999-03-03 2002-02-05 Denso Corporation Total-focus imaging apparatus using a variable-focus lens
US6724419B1 (en) * 1999-08-13 2004-04-20 Universal Imaging Corporation System and method for acquiring images at maximum acquisition rate while asynchronously sequencing microscope devices
WO2003012518A2 (fr) * 2001-07-31 2003-02-13 Interscope Technologies, Inc. Systeme servant a creer des images microscopiques de montage numerique
US20050089208A1 (en) * 2003-07-22 2005-04-28 Rui-Tao Dong System and method for generating digital images of a microscope slide
EP1895347A1 (fr) * 2006-09-01 2008-03-05 Universität Zürich Microscope à balayage et procédé de fonctionnement d'un microscope à balayage

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9715095B2 (en) 2011-10-11 2017-07-25 Carl Zeiss Microscopy Gmbh Microscope and method for SPIM microscopy

Also Published As

Publication number Publication date
DE102008038359A1 (de) 2010-02-25

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