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WO2010050208A1 - Liquid flow path device and method of manufacturing same - Google Patents

Liquid flow path device and method of manufacturing same Download PDF

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Publication number
WO2010050208A1
WO2010050208A1 PCT/JP2009/005711 JP2009005711W WO2010050208A1 WO 2010050208 A1 WO2010050208 A1 WO 2010050208A1 JP 2009005711 W JP2009005711 W JP 2009005711W WO 2010050208 A1 WO2010050208 A1 WO 2010050208A1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid
tank
flow path
substrate
layer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2009/005711
Other languages
French (fr)
Japanese (ja)
Inventor
高橋茂
櫻井正明
若松二郎
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujikura Kasei Co Ltd
Original Assignee
Fujikura Kasei Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2008276468A external-priority patent/JP5228797B2/en
Priority claimed from JP2009194590A external-priority patent/JP5446597B2/en
Priority claimed from JP2009194592A external-priority patent/JP5229158B2/en
Priority claimed from JP2009194591A external-priority patent/JP5359686B2/en
Priority to CN2009801360340A priority Critical patent/CN102150048B/en
Application filed by Fujikura Kasei Co Ltd filed Critical Fujikura Kasei Co Ltd
Priority to EP09823321.6A priority patent/EP2352036B1/en
Priority to US12/739,325 priority patent/US8499794B2/en
Publication of WO2010050208A1 publication Critical patent/WO2010050208A1/en
Anticipated expiration legal-status Critical
Priority to US13/934,310 priority patent/US9283561B2/en
Priority to US13/934,320 priority patent/US9283562B2/en
Priority to US13/934,314 priority patent/US9579653B2/en
Ceased legal-status Critical Current

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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/56—Labware specially adapted for transferring fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0093—Microreactors, e.g. miniaturised or microfabricated reactors
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    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
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    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
    • F16K—VALVES; TAPS; COCKS; ACTUATING-FLOATS; DEVICES FOR VENTING OR AERATING
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    • F16K2099/0073—Fabrication methods specifically adapted for microvalves
    • F16K2099/008—Multi-layer fabrications
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10T137/0491—Valve or valve element assembling, disassembling, or replacing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10T156/1002—Methods of surface bonding and/or assembly therefor with permanent bending or reshaping or surface deformation of self sustaining lamina

Definitions

  • the present invention relates to a plate-like liquid flow path device suitably used for detecting and analyzing antigens in blood, for example, and a method for producing the same.
  • This application includes Japanese Patent Application No. 2008-276468 filed in Japan on October 28, 2008, Japanese Patent Application No. 2009-194590 filed in Japan on August 25, 2009, and Japanese Application on August 25, 2009. Claimed priority based on Japanese Patent Application No. 2009-194591 filed and Japanese Patent Application No. 2009-194592 filed in Japan on Aug. 25, 2009, the contents of which are incorporated herein by reference.
  • a plurality of flow paths are formed in the radial direction of a rotatable disc, an antibody is fixed in advance in a part of the flow paths, and then body fluid is circulated through the flow paths.
  • a disk-shaped liquid flow path device is disclosed in which an antigen in a body fluid is captured by an antibody by an antigen-antibody reaction.
  • the liquid flow path device of Patent Document 1 needs to use a separate micropump, and the liquid device of Patent Document 2 requires a device for rotating the disk. there were. Further, in such a conventional liquid channel device, the liquid channel cannot be closed or opened, which may cause inconvenience in detection and analysis of the target component. In addition, in such a conventional liquid flow path device, a part of the liquid flow path cannot be closed from the closed state to the open state, from the open state to the closed state, or to be closed or opened. Inconvenient analysis may occur.
  • the object of the present invention is to make it possible to easily change the liquid flow path from the closed state to the open state without requiring a separate device for flowing the liquid, that is, to allow the liquid to flow through the flow path easily and smoothly. It is to provide a liquid channel device at a low cost. Furthermore, by providing a liquid flow path device that can easily change the liquid flow path from the closed state to the open state, from the open state to the closed state, from the open state to the closed state, or from the closed state to the open state, at low cost. is there.
  • a liquid channel in which a liquid consisting of at least one of a sample and a reagent flows and at least one liquid tank in which the liquid is accumulated are formed on at least one surface of the substrate
  • the liquid flow path device of the present invention further includes an opening part that changes a part of the liquid flow path from a closed state to an open state, and a closing part that changes the open state from the open state to the closed state.
  • a first base layer constituting the surface of the plate, a strong adhesive layer formed inside the first base layer, a second base layer formed inside the strong adhesive layer, and the second A weak adhesive layer that is formed inside the base material layer and adheres to the flow path forming surface, and in the opening portion, a first convex portion is formed in the liquid flow channel, and a top portion of the first convex portion And the weak adhesive layer adhere to each other, and the strong adhesive layer and the second base material layer are separated from each other, and in the closed portion, a second convex portion is formed in the liquid channel, and the second convex
  • the top of the part and the weak adhesive layer are separated from each other, and a spacer member is interposed between the strong adhesive layer and the second base material layer, and the spacer member and the strong adhesive layer adhere to
  • the spacer member is interposed between the strong adhesive layer and the second base layer, it is preferable that the said spacer member and said strong adhesive layer is adhered.
  • the substrate includes an outer layer, an intermediate layer laminated on the inner side of the outer layer, and an inner layer laminated on the inner side of the intermediate layer.
  • the inner layer includes an upper part of the liquid tank, the liquid channel, It is preferable that a 1st convex part and the said 2nd convex part are formed, and the lower part of the said liquid tank is formed in the said intermediate
  • the substrate includes an outer layer and an inner layer laminated on the inner side of the outer layer, and the inner layer includes the liquid tank, the liquid flow path, the first convex portion, and the second convex portion.
  • the liquid tank provided with the liquid feeding part is provided with a backflow prevention part for preventing the backflow of the liquid fed by the liquid feeding part.
  • the backflow prevention part is preferably formed in the inner layer.
  • a liquid flow path in which a liquid consisting of at least one of a sample and a reagent flows is formed on at least one surface of the substrate, and the flow path formation in which the liquid flow path of the substrate is formed.
  • a liquid channel device in which a cover plate is stacked on a surface the liquid channel device having an opening portion that opens a part of the liquid channel from a closed state to an open state, and the cover plate constitutes a surface of the cover plate
  • the liquid flow path device further includes a closing portion that changes a part of the liquid flow path from an open state to a closed state, and in the closing portion, a second protrusion is formed in the liquid flow path, and the second protrusion
  • a spacer member is interposed between the strong adhesive layer and the second base material layer, and the spacer member and the strong adhesive layer adhere to each other.
  • a measuring tank for measuring a certain amount of liquid is provided in the liquid channel, the closing part is provided at least upstream of the measuring tank, and the opening part is provided downstream. It is preferable that the weighing tank is provided with an overflow portion for overflowing the liquid exceeding the certain amount.
  • a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate.
  • the method for manufacturing a liquid channel device is a method for manufacturing the liquid channel device, wherein a first step of forming the liquid channel and the liquid tank on the substrate; A second step of partially forming the plug body and a third step of laminating the cover plate on the flow path forming surface of the substrate, wherein the first step constitutes an inner layer of the substrate An upper part of the liquid tank and the liquid channel are formed on a sheet, and a lower part of the liquid tank is formed on a sheet constituting the intermediate layer of the substrate, and then a sheet constituting the inner layer and the intermediate layer are formed. And a sheet constituting the outer layer of the substrate are sequentially laminated.
  • it is preferable to form the plug body by a method of applying a plug body forming material for forming the plug body to the part of the liquid channel.
  • a liquid flow path through which liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate, and the liquid flow path of the substrate and the liquid flow path A liquid flow path device in which a cover plate is laminated on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having a closing portion that changes a part of the liquid flow path from an open state to a closed state, and the closing portion A sealing material supply tank formed by branching from the part of the liquid flow path, and a portion of the lid plate or the sealing member filled in the sealing material supply tank and corresponding to the sealing material supply tank.
  • the method for manufacturing a liquid channel device of the present invention is a method for manufacturing the liquid channel device, wherein the liquid channel, the liquid tank, and the sealing material supply tank are formed on the substrate. And a second step of filling the sealing material supply tank with the sealing material, and a third step of laminating the lid plate on the flow path forming surface of the substrate.
  • the first step After forming the upper part of the liquid tank, the liquid flow path and the sealing material supply tank on the sheet constituting the inner layer of the substrate, and forming the lower part of the liquid tank on the sheet constituting the intermediate layer of the substrate, The sheet constituting the inner layer, the sheet constituting the intermediate layer, and the sheet constituting the outer layer of the substrate are sequentially laminated.
  • the second step it is preferable to fill the sealing material by a method of applying the sealing material to the sealing material supply tank.
  • a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate.
  • a liquid flow path device in which a cover plate is laminated on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having an opening portion that changes a part of the liquid flow path from a closed state to an open state, and the opening portion Includes a stopper disposed in the part of the liquid flow path, and a recessed portion capable of accommodating the plug body at an inner surface of the lid plate or a position opposite to the plug body on the bottom of the liquid flow path.
  • the plug body is moved from the part of the liquid flow path into the recess by the operation of pressing the lid plate or the bottom part from the outside to be in the open state. .
  • the method for manufacturing a liquid channel device is a method for manufacturing a liquid channel device in which the concave portion is formed on the inner surface of the lid plate, and the liquid channel, the liquid tank, A first step of forming the recess in the lid plate, a second step of forming a plug in the part of the liquid channel, and the lid plate on the channel forming surface of the substrate
  • the first step the upper part of the liquid tank and the liquid flow path are formed in a sheet constituting the inner layer of the substrate, and the sheet constituting the intermediate layer of the substrate
  • the lower part of the liquid tank is formed on the sheet, the sheet constituting the inner layer of the substrate, the sheet constituting the intermediate layer of the substrate, and the sheet constituting the outer layer of the substrate are sequentially laminated, Forming the liquid flow path and the liquid tank on a substrate, forming a sheet constituting the inner layer of the lid plate After forming the serial recess, the sheet constituting the inner layer of the cover plate, by laminating the sheet constituting the outer
  • the method for manufacturing a liquid channel device of the present invention is a method for manufacturing a liquid channel device in which the concave portion is formed at the bottom of the liquid channel, and the liquid channel and the liquid tank are formed on the substrate. And a first step of forming the concave portion, a second step of forming a plug at a position of the inner surface of the lid plate facing the concave portion, and laminating the lid plate on the flow path forming surface of the substrate.
  • a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate.
  • a liquid flow path device in which a cover plate is stacked on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having a closing portion that changes a part of the liquid flow path from an open state to a closed state, and the closing portion Comprises a stopper housed in a recess formed in the inner surface of the lid plate or the bottom of the liquid flow path, and the stopper body is formed by pressing the lid plate or the bottom portion from outside. It moves to the said part of the said liquid flow path from the inside, It is set as the said closed state.
  • the method for manufacturing a liquid channel device of the present invention is a method for manufacturing a liquid channel device in which the recess is formed on the inner surface of the lid plate, and the liquid channel and the liquid tank are formed on the substrate.
  • an upper part of the liquid tank and the liquid channel are formed in a sheet constituting the inner layer of the substrate, and a lower part of the liquid tank is formed in the sheet constituting the intermediate layer of the substrate.
  • the sheet constituting the inner layer of the substrate, the sheet constituting the intermediate layer of the substrate, and the sheet constituting the outer layer of the substrate are sequentially laminated, and the liquid flow path is formed on the substrate.
  • the liquid tank was formed, and the recess was formed in a sheet constituting the inner layer of the lid plate , A sheet constituting the inner layer of the cover plate, by laminating the sheet constituting the outer layer of the cover plate, and forming the recess in the cover plate.
  • the method for manufacturing a liquid channel device of the present invention is a method for manufacturing a liquid channel device in which the concave portion is formed at the bottom of the liquid channel, and the liquid channel, the liquid tank, A first step of forming the concave portion, a second step of forming a plug in the concave portion, and a third step of laminating the lid plate on the flow path forming surface of the substrate.
  • an upper part of the liquid tank and the liquid flow path are formed in a sheet constituting the inner layer of the substrate, and an intermediate part and the concave part of the liquid tank are formed in a sheet constituting the inner intermediate layer of the substrate.
  • the plug is formed by a method of applying a plug forming material for forming the plug.
  • the liquid channel can be easily changed from the closed state to the open state without requiring a separate device for circulating the liquid, that is, the liquid can be easily and smoothly circulated through the channel.
  • a liquid channel device can be provided at low cost.
  • a liquid flow path device that can easily change the liquid flow path from the closed state to the open state, from the open state to the closed state, or from the open state to the closed state, or from the closed state to the open state, at low cost. Can be provided.
  • FIG. 2 is an enlarged plan perspective view of a part of the liquid channel device in FIG. 1.
  • FIG. 3 is a cross-sectional view taken along the line I-I ′ of FIG. 2.
  • FIG. 2 is an explanatory diagram for explaining a state in which an opening portion operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an explanatory diagram for explaining a state in which an opening portion operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an explanatory diagram for explaining a state in which a closing portion operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an explanatory diagram for explaining a state in which a closing portion operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an explanatory diagram for explaining a state in which a closing portion operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an explanatory diagram for explaining a state in which a liquid feeding section operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an explanatory diagram for explaining a state in which a liquid feeding section operates in the liquid channel device of FIG. 1.
  • FIG. 2 is an enlarged perspective view of a part of a substrate of the liquid channel device in FIG. 1.
  • It is a schematic plane perspective view which shows 2nd Embodiment of the liquid flow-path apparatus of this invention.
  • FIG. 15 is a plan perspective view in which a part of the liquid channel device in FIG. 14 is enlarged.
  • FIG. 16 is a cross-sectional view taken along the line I-I ′ of FIG.
  • FIG. 14 In the liquid channel device of FIG. 14, it is a figure explaining a mode that an opening part act
  • FIG. 16 is a cross-sectional view taken along the line II-II ′ of FIG. 15. In the liquid channel device of FIG. 14, it is a figure explaining a mode that a closing part act
  • FIG. 14 it is a figure explaining a mode that a closing part act
  • FIG. 15 is a process diagram schematically illustrating a manufacturing method of the liquid channel device in FIG. 14. It is a schematic plane perspective view which shows the liquid channel apparatus of 5th Embodiment.
  • FIG. 22 is an enlarged plan perspective view of a part of the liquid channel device of FIG. 21.
  • FIG. 23 is a cross-sectional view taken along the line I-I ′ of FIG. 22. It is an expanded sectional view of the opening part in FIG. It is an expanded sectional view of the closing part in FIG. In the liquid channel device of FIG.
  • FIG. 21 is a diagram illustrating a state in which the closing portion operates in the liquid channel device of FIG.
  • FIG. 21 is a cross-sectional view illustrating a state where a load is removed.
  • FIG. 22 is a process diagram schematically illustrating a manufacturing method of the liquid channel device in FIG. 21.
  • FIG. 22 is a cross-sectional view showing a state in which a stopper receiver is provided in the liquid channel device of FIG. 21. It is a top view which shows typically the example which made the planar view shape of the plug body the rhombus. It is sectional drawing which shows the part about the liquid flow-path apparatus of 6th Embodiment. It is an expanded sectional view of the opening part in FIG. It is an expanded sectional view of the closing part in FIG. In the liquid channel device of FIG.
  • FIG. 31 is a process diagram schematically illustrating a manufacturing method of the liquid channel device in FIG. 30.
  • FIG. 1 is a plan perspective view schematically showing the liquid channel device 10A of the first embodiment
  • FIG. 2 is a plan perspective diagram showing an enlarged part of the liquid channel device 10A of FIG. 1
  • FIG. 6 is a cross-sectional view taken along the line ⁇ I ′.
  • This liquid flow path device 10A includes a groove-shaped liquid flow path 12 through which a liquid consisting of at least one of a sample and a reagent circulates on one side of a flat substrate 11A made of a flat plate, and an end portion or a middle of the liquid flow path 12 A plurality of (9 in this example) liquid tanks (14a to 14i) in which liquid is accumulated are formed, and a flow path forming surface 12a on the side where the liquid flow path 12 and the liquid tanks (14a to 14i) of the substrate 11A are formed.
  • a cover plate 13 is laminated. Even if the liquid channel device 10A of this example is erected so that the upper end side in FIG.
  • the sample circulates in the direction of arrow F toward the portion by gravity, and the sample is mixed with various treatments and reagents in the middle of the sample to prepare a measurement solution for various detections and analyses.
  • the liquid can be easily and smoothly circulated by using the liquid feeding part of the liquid tank in combination.
  • a sample loading tank 14a in which the loaded sample is collected, and the sample flowing from the sample loading tank 14a is filtered downstream of the sample loading tank 14a.
  • a filtration tank 14b containing a filter (not shown) to be processed is provided downstream of the filtration tank 14b.
  • a measuring tank 14c Downstream of the filtration tank 14b, there is provided a measuring tank 14c whose inner volume is formed in a predetermined amount and capable of weighing the filtered sample.
  • the weighing tank 14c in this example is provided with an overflow portion composed of an overflow channel 12b and a waste liquid tank 14d provided downstream thereof. Therefore, the sample exceeding a certain amount in the measuring tank 14c overflows and flows through the overflow channel 12b and flows into the waste liquid tank 14d. As a result, a certain amount of sample can be measured in the measuring tank 14c.
  • a first mixing tank 14f Downstream of the measuring tank 14c, a first mixing tank 14f is provided in which the sample weighed in the measuring tank 14c and the liquid first reagent previously sealed in the first reagent tank 14e are mixed. Downstream of the first mixing tank 14f is a second mixing in which the intermediate preparation liquid prepared in the first mixing tank 14f and the liquid second reagent sealed in a predetermined amount in the second reagent tank 14g are mixed.
  • a tank 14h is provided.
  • a measuring tank 14i is provided downstream of the second mixing tank 14h, and the measurement liquid prepared in the second mixing tank 14h is stored in the measuring tank 14i. Analysis is performed.
  • a mixing function tank 14d communicating with the measurement tank 14i and the liquid channel 12 is provided downstream of the measurement tank 14i.
  • the measurement liquid is sufficiently stirred and mixed by repeating the mixing operation of returning the measurement liquid in the measurement tank 14i to the mixing function tank 14d and then returning to the measurement tank 14i as necessary. It becomes a state more suitable for detection and analysis.
  • Each liquid tank is provided with a communication hole (not shown) that can be opened and closed and communicates with the atmosphere.
  • the lid plate 13 of the liquid flow path device 10A includes a first base layer 13a constituting the surface of the lid plate 13, and a strong adhesive layer formed on the inner side of the first base layer 13a. 13b, a second base material layer 13c formed inside the strong adhesive layer 13b, and a weak adhesive layer 13d formed inside the second base material layer 13c and sticking to the flow path forming surface 12a.
  • the first base material layer 13a bends when a load in the vertical direction (a direction perpendicular to the first base material layer 13a) is applied from the surface side, and then returns to the original when the load is removed. It is made of a material that has a restoring force to return to.
  • the second base material layer 13c is made of a material that is easily bent by the same load and does not recover even when the load is removed, that is, easily plastically deformed. Moreover, the adhesive force of the strong adhesion layer 13b is formed larger than the weak adhesion layer 13d.
  • the liquid channel device 10A includes opening portions S1 to S7 that make a part of the liquid channel 12 from the closed state to the open state, and a closing portion T1 that changes the open state to the closed state.
  • the opening sections S1 to S7 are provided between the sample feeding tank 14a and the filtration tank 14b, between the filtration tank 14b and the weighing tank 14c, between the weighing tank 14c and the first mixing tank 14f, Between the tank 14f and the second mixing tank 14h, between the first reagent tank 14e and the first mixing tank 14f, between the second reagent tank 14g and the second mixing tank 14h, and between the second mixing tank 14h and the measuring tank One is provided in each liquid flow path 12 between 14i.
  • the closing part T1 is provided on the downstream side of the opening part S2 in the liquid flow path 12 between the filtration tank 14b and the measuring tank 14c.
  • the first convex portion 15 is formed in the liquid flow path 12, and the top portion 15a of the first convex portion 15 is weakly adhered.
  • the layer 13d adheres, and the strong adhesion layer 13b and the second base material layer 13c are separated from each other. Therefore, the liquid flow path 12 in each of the opening portions S1 to S7 is closed by the first convex portion 15 and the weak adhesive layer 13d adhered to the top portion 15a, and is normally closed.
  • the first base material layer 13a in the opening portion S1 is pressed from the surface side as indicated by the arrow A, and the first base material layer 13a is pressed.
  • the first base material layer 13a bends and the strong adhesive layer 13b inside the first base material layer 13a sticks to the second base material layer 13c, as shown in FIG. 4A.
  • the first base material layer 13a is restored to its original state by its restoring force, and at that time, the strong adhesive sticking to the inside of the first base material layer 13a.
  • the second base material layer 13c that adheres to the layer 13b, the strong adhesive layer 13b, and can be easily plastically deformed, and the weak adhesive layer 13d that adheres to the inside of the second base material layer 13c are also restored to the first base material layer 13a.
  • the top portion 15a of the first convex portion 15 and the weak adhesive layer 13d are newly separated, and the liquid flows therethrough.
  • the cover plate 13 is pressed from the surface side to apply a load in the vertical direction, and then the pressing operation for removing the load removes the load from the first convex portion 15 that was originally adhered.
  • the top portion 15a and the weak adhesive layer 13d are separated from each other, and as a result, the liquid channel 12 in this portion is changed from the closed state to the open state.
  • a second convex portion 16 is formed in the liquid flow path 12, and the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are separated from each other, and A spacer member 17 is interposed between the strong adhesion layer 13b and the second base material layer 13c, and the spacer member 17 and the strong adhesion layer 13b are adhered. Therefore, in the liquid flow path 12 in the closing part T1, the top part 16a of the 2nd convex part 16 and the weak adhesion layer 13d are separated, the flow path is maintained, and it is an open state normally. However, as shown in FIG.
  • the spacer member 17 is not adhered between the second base material layer 13c and the second base material layer 13c can be easily plastically deformed.
  • the material layer 13c and the weak adhesive layer 13d do not follow the restoration of the first base material layer 13a.
  • the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are in an adhesive state, and the liquid channel 12 is closed, so that the liquid cannot flow therethrough.
  • the closing portion T1 the top plate 16a of the second convex portion 16 that was originally separated by the pressing operation of removing the load after pressing the cover plate 13 from the surface side and applying a vertical load.
  • the weak adhesive layer 13d are adhered and closed, and as a result, the liquid flow path 12 in this portion is changed from the open state to the closed state.
  • the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, the measuring tank 14i, and the mixing function tank 14d are configured to liquidate liquid in each liquid tank.
  • Each has liquid feeding parts P1 to P5 for feeding liquid outside the tank.
  • the liquid feeding part P1 of the measuring tank 14c sends the liquid in the measuring tank 14c to the downstream side, that is, the first mixing tank 14f.
  • the liquid feeding part P2 of the first mixing tank 14f feeds the liquid in the first mixing tank 14f to the downstream side, that is, the second mixing tank 14h.
  • the liquid feeding part P3 of the second mixing tank 14h feeds the liquid in the second mixing tank 14h to the downstream side, that is, the measurement tank 14i. Moreover, the liquid feeding part P4 of the measuring tank 14i sends the liquid in the measuring tank 14i to the downstream side, that is, the mixing function tank 14d. On the other hand, the liquid feeding part P5 of the mixing function tank 14d returns the liquid in the mixing function tank 14d to the upstream side, that is, the measurement tank 14i.
  • the strong adhesive layer 13b and the second substrate are not spaced apart from the layer 13c, but the spacer member 17 and the strong adhesion layer 13b are adhered to each other, and the layers are densely formed. Therefore, as shown in FIGS. 6A and 6B by taking the liquid feeding part P1 as an example, when the lid plate 13 of this portion is pressed in the direction indicated by the arrow C from the outside (FIG. 6B), the lid plate of the pressed portion. 13 bends inward.
  • the internal volume of the measuring tank 14c becomes small, the liquid in the measuring tank 14c is discharged and fed, and the action as the liquid feeding part P1 is expressed.
  • the strong adhesion layer 13b and the second base material layer 13c are separated from each other, the spacer member 17 is not interposed, and the interlayer is not dense, the cover plate 13 of this portion is pressed from the outside.
  • the internal volume of the measuring tank 14c is not reduced only by the strong adhesion layer 13b sticking to the second base material layer 13c. In that case, the action as a liquid feeding part does not appear.
  • the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, and the measuring tank 14i each provided with such liquid feeding sections P1 to P4 are shown in FIGS.
  • FIG. 2 there are also provided backflow prevention units G1 to G6 for preventing the backflow of the liquid fed by the liquid feeding units P1 to P4 to the upstream side. Therefore, when the liquid feeding sections P1 to P4 are operated, the liquid in each liquid tank does not flow backward on the upstream side but is fed only on the downstream side.
  • the backflow prevention parts G1 to G6 are constituted by a flexible weir plate 18.
  • the weir plate 18 is a weir plate at a boundary portion between the measuring tank 14c and the liquid flow path 12 on the upstream side of the measuring tank 14c. Only the base end 18b is fixed to the bottom of the liquid flow path 12 so that the tip 18a of the 18 is inclined to the downstream side, and the tip 18a and both side ends are not fixed. Therefore, in FIG. 6A, FIG. 6B and FIG.
  • the mixing function tank 14d is provided in order to sufficiently stir and mix the measurement liquid by moving the measurement liquid back and forth between the measurement tank 14i on the upstream side. Therefore, it is not necessary to provide the backflow prevention part which prevents the backflow of the liquid to the upstream side in the mixing function tank 14d.
  • the liquid channel device 10A As a specific method for preparing the measurement liquid using the liquid channel device 10A, first, the liquid channel device 10A is set up so that the sample introduction tank 14a side is positioned upward and the measurement tank 14i side is positioned downward. Thus, the liquid is likely to flow from the upstream side to the downstream side by gravity. Next, the sample is sampled into a syringe or the like, and the needle of this syringe is pierced into the cover plate 13 corresponding to the sample loading tank 14a to inject the sample into the sample loading tank 14a.
  • the opening portion S1 provided between the sample charging tank 14a and the filtration tank 14b is pressed as described above, that is, an operation of pressing the first base material layer 13a from the surface side to apply a load and then removing it.
  • the liquid channel 12 in this portion is opened, and the sample is introduced to the filtration tank 14b by gravity.
  • the pressing operation may be performed manually by the operator manually pressing the first base material layer 13a from the surface side with a finger, or using a pressing device in which the pressing position is pre-programmed as XY coordinates, You may make it push a predetermined position.
  • the opening portion S2 provided between the filtration tank 14b and the measuring tank 14c is operated by pressing operation, and the liquid flow path 12 of this portion is opened,
  • the sample is introduced into the weighing tank 14c by gravity.
  • the closing portion T1 provided between the filtration tank 14b and the measuring tank 14c is operated by a pressing operation, and the liquid flow in this portion is measured.
  • the path 12 is closed.
  • the opening portion S3 provided downstream of the measuring tank 14c is operated by a pressing operation, and the liquid flow in this portion
  • the path 12 is opened.
  • the lid plate 13 that closes the measuring tank 14c is pressed from the outside to operate the liquid feeding part P1, and the sample after weighing is fed into the first mixing tank by the action of gravity and the action of the liquid feeding part P1. 14f.
  • the opening portion S4 between the first reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, and the first reagent is moved into the first mixing tank 14f.
  • the sample is introduced into 14f, and the sample and the first reagent are mixed in the first mixing tank 14f to prepare an intermediate preparation solution.
  • the opening part S5 between the first mixing tank 14f and the second mixing tank 14h is operated by a pressing operation, and the liquid flow path 12 in this part is opened.
  • the liquid feeding part P2 is operated in the same manner as the liquid feeding part P1, and the intermediate preparation liquid prepared in the first mixing tank 14f is moved to the second mixing tank 14h by the action of gravity and the action of the liquid feeding part P2.
  • the opening part S6 between the second reagent tank 14g and the second mixing tank 14h is operated by a pressing operation to introduce the second reagent into the second mixing tank 14h, and the intermediate preparation solution and the second reagent are supplied to the second reagent tank 14g. 2 Mix in the mixing tank 14h to prepare a measurement solution.
  • the opening part S7 between the second mixing tank 14h and the measuring tank 14i is operated by pressing, so that the liquid flow path 12 in this part is opened, and then the liquid feeding part P3 is operated, and gravity is applied.
  • the measuring liquid prepared in the second mixing tank 14h is introduced into the measuring tank 14i by the action of and the action of the liquid feeding part P3.
  • the liquid feeding unit P4 is operated to temporarily feed the measurement liquid in the measurement tank 14i to the mixing function tank 14d.
  • the liquid feeding part P5 is operated to return the measurement liquid in the mixing function tank 14d to the measurement tank 14i. Such a mixing operation is repeated as necessary, and the measurement liquid is sufficiently stirred and mixed.
  • the entire liquid flow path device 10A is supplied to the detection analysis unit, and the target liquid is detected and measured in the measurement tank 14i. Measure.
  • the liquid feeding parts P1 to P5 are operated as described above and the liquid is circulated through the liquid flow path 12 of the liquid flow path device 10A, as shown in FIG. It is preferable that the communication hole is appropriately opened and closed so that the liquid flows more smoothly.
  • the communication hole provided in the measuring tank 14c is changed from the closed state to the open state, Thereafter, by releasing the pressure, it is possible to prevent the liquid in the measuring tank 14c from being reduced in pressure and flowing downstream to flow back into the measuring tank 14c.
  • the liquid feeding parts P1 to P5 for feeding the liquid in the liquid tank include the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, and the measuring tank. 14i and the mixing function tank 14d are respectively provided, so that the liquid is circulated even if the sample, the intermediate preparation liquid, and the measurement liquid are difficult to flow through the liquid flow path 12 due to their viscosity. Therefore, the liquid can be easily and smoothly circulated without requiring a separate device.
  • the liquid feeding parts P1 to P5 in this example are configured using the lid plate 13 of the liquid flow path device 10A, it is not necessary to prepare another member for the liquid feeding parts P1 to P5. It is low cost and simple in construction. Further, since the liquid feeding parts P1 to P5 are also operated by a simple operation only by pressing, the operability is excellent.
  • the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, and the measuring tank 14i provided with the liquid feeding parts P1 to P4, respectively, are the weir plates as the backflow prevention parts G1 to G6. 18 is provided, the liquid does not flow backward to the upstream side when the liquid feeding parts P1 to P4 are operated.
  • the liquid channel device 10A of this example includes the opening portions S1 to S7 that make the liquid channel 12 from the closed state to the open state and the closing portion T1 that makes the liquid channel 12 from the open state to the closed state.
  • a closing portion T1 is provided upstream of the measuring tank 14c, and an opening portion S3 is provided downstream. Therefore, the sample can be accurately and quickly measured in the measuring tank 14c and introduced into the first mixing tank 14f.
  • the opening part S3 is not provided downstream of the measuring tank 14c, and the liquid channel 12 in this part is always open, the sample is continuously transferred from the measuring tank 14c even during measurement.
  • the closing portion T1 is not provided upstream of the measuring tank 14c, a filtration tank may be used depending on the amount of the sample injected into the sample feeding tank 14a even after a certain amount of sample is stored in the measuring tank 14c.
  • the sample that has passed through 14b continues to flow into the weighing tank 14c, and the weighing itself may still be difficult.
  • the closing portion T1 when the closing portion T1 is provided upstream of the measuring tank 14c, the entire amount of the sample that has passed through the filtration tank 14b does not end completely flowing into the measuring tank 14c, but the measuring tank 14c.
  • the closing portion T1 can be operated to stop further inflow of the sample into the weighing tank 14c, and the sample can be accurately and quickly measured.
  • an opening S5 is provided between the first mixing tank 14f and the second mixing tank 14h
  • an opening S7 is provided between the second mixing tank 14h and the measurement tank 14i. Therefore, in the first mixing tank 14f and the second mixing tank 14h, after the target mixing and reaction have sufficiently progressed, the opening parts S5 and S7 are opened, and then the liquid feeding parts P2 and P3 are operated.
  • the intermediate preparation liquid and the measurement liquid can be introduced into the second mixing tank 14h and the measurement tank 14i, respectively. Therefore, it is possible to prevent a decrease in detection and analysis accuracy due to insufficient mixing and reaction.
  • the opening portions S4 and S6 are provided between the first reagent tank 14e and the first mixing tank 14f and between the second reagent tank 14g and the second mixing tank 14h, it is desirable. These are opened at the point of time, and the first reagent and the second reagent previously sealed in the first reagent tank 14e and the second reagent tank 14g, respectively, are caused to flow into the first mixing tank 14f and the second mixing tank 14h. Can do. If the opening portions S4 and S6 are not provided, the first reagent and the second reagent may start to flow downstream when the liquid channel device 10A is stored.
  • the opening portions S1 to S7 and the closing portion T1 of the liquid channel device 10A in this example are a combination of the first convex portion 15 and the second convex portion 16 formed in the liquid channel 12 and the lid plate 13. Therefore, it is not necessary to prepare another member for opening and closing the liquid flow path 12, and the cost is low and the configuration is simple. In addition, the opening and closing operations can be performed only by a simple pressing operation, and the operability is excellent.
  • the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14f, the measuring tank 14i, and the mixing function tank 14d respectively supply the liquid feeding parts P1 to P5. It was set as the form which has. However, all these liquid tanks may not have a liquid feeding part, and other liquid tanks other than these may have a liquid feeding part. That is, it is possible to appropriately determine which liquid tank is provided with the liquid feeding unit according to the type and characteristics of the sample and the reagent. For example, in this example, when the measurement liquid is prepared using the liquid flow path device 10A, first, the liquid flow path device 10A is positioned so that the sample introduction tank 14a side is positioned upward and the measurement tank 14i side is positioned downward.
  • the liquid flow path device can be configured so that the liquid can be fed without using gravity by providing the liquid feeding section in all the liquid tanks.
  • the liquid channel device 10A nine liquid tanks are formed.
  • the type, number, order of arrangement, and the like of the liquid tanks can be appropriately set according to the purpose.
  • the weir plate 18 is used as the backflow prevention units G1 to G6 disposed in the liquid tanks provided with the liquid feeding units P1 to P4.
  • the barrier plate 18 may be arranged in series so that the effect of preventing the reverse flow can be further obtained. May be used in combination.
  • the form in which the closing part is used for the purpose of preventing the backflow for example, employs a measuring tank equipped with an overflow part composed of an overflow channel communicating with the measuring tank and a waste liquid tank provided downstream thereof. This is particularly effective when In such a measuring tank, a sample exceeding a certain amount in the measuring tank overflows, flows through the overflow channel, and flows into the waste liquid tank.
  • a certain amount of sample can be measured in the measuring tank. Therefore, it is necessary for the sample to flow smoothly from the measuring tank to the overflow channel when measuring.
  • the liquid feeding section provided in the measuring tank is operated to send the sample after measurement to the downstream side, it is necessary to prevent the sample from flowing into the overflow channel from the measuring tank.
  • a backflow prevention unit cannot be provided at a location where the sample needs to flow in both directions. Therefore, it is preferable to provide a closing portion at such a location so that the location can be closed only when necessary.
  • FIG. 8 shows a second embodiment of the liquid flow path device of the present invention.
  • This liquid flow path device 10B is a liquid flow path device 10A described above on one side of a substrate 11B made of a fan-shaped flat plate.
  • a groove-like liquid channel 12 through which a liquid composed of at least one of a sample and a reagent flows, and a plurality of liquid tanks (14a, 14e to 14h, 14J) in which the liquid is accumulated at the end or in the middle of the liquid channel 14k, 14m), and the cover plate 13 is laminated on the flow path forming surface 12a of the substrate 11 on the side where the liquid flow path 12 is formed.
  • the liquid channel device 10B when rotated about the upper end side in FIG. 8, in the direction of arrow F ′ from the upstream end of the liquid channel 12 toward the downstream end.
  • the sample is circulated by centrifugal force, and the sample is mixed with various treatments and reagents in the middle of the sample to obtain a measurement solution.
  • the upstream end portion of the liquid flow path 12 is provided with a sample introduction tank 14a in which the introduced sample is stored, and the first reagent and the first reagent from the first reagent tank 14e are provided downstream of the sample introduction tank 14a.
  • a first mixing tank 14f in which the second reagent from the second reagent tank 14g and the sample from the sample charging tank 14a are mixed to prepare an intermediate preparation solution.
  • Downstream of the first mixing tank 14f is a second mixing in which the third reagent from the third reagent tank 14j, the fourth reagent from the fourth reagent tank 14k, and the intermediate preparation liquid from the first mixing tank 14f are mixed.
  • a tank 14h is provided downstream of the first mixing tank 14f.
  • the second mixing tank 14h also functions as a measurement tank, and various components are detected and analyzed by a detection analysis unit (not shown) with respect to the measurement liquid prepared in the second mixing tank 14h.
  • a disposal tank 14m is formed for storing the measurement liquid after being measured in the second mixing tank 14h.
  • Each liquid tank is provided with a communication hole (not shown) that communicates with the atmosphere.
  • the lid plate 13 has the same configuration as shown in FIG. 3 as in the case of the above-described liquid channel device 10A, that is, the first base material layer 13a constituting the surface of the lid plate 13 and A strong adhesive layer 13b formed inside the first base material layer 13a, a second base material layer 13c formed inside the strong adhesive layer 13b, and formed inside the second base material layer 13c. It has a weak adhesive layer 13d that adheres to the path forming surface.
  • the sample loading tank 14a and the first mixing tank 14f between the sample loading tank 14a and the first mixing tank 14f, between the first sample tank 14e and the first mixing layer 14f, between the second sample tank 14g and the first mixing tank 14f, the first mixing tank. 14f and the second mixing tank 14h, the third sample tank 14j and the second mixing layer 14h, the fourth sample tank 14k and the second mixing tank 14h, the second mixing tank 14h and the disposal tank 14m.
  • a closing part T2 is provided on the downstream side of the opening part S8.
  • the first convex portion 15 is formed in the liquid channel 12, and the first convex portion 15 The top portion 15a and the weak adhesive layer 13d are adhered to each other, and the strong adhesive layer 13b and the second base material layer 13c are separated from each other.
  • the second convex portion 16 is formed in the liquid flow path 12, the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are separated from each other, and the strong adhesive layer 13b and the second base are formed.
  • a spacer member 17 is interposed between the material layer 13c and the spacer member 17 and the strong adhesion layer 13b are adhered.
  • the liquid channel device 10B When preparing a measurement liquid using the liquid channel device 10B, first, the liquid channel device 10B is positioned on the rotation center side on the sample loading tank 14a side, and the measurement tank 14i side is positioned on the outer peripheral side of the rotation. Set to centrifuge. Next, the sample is sampled into a syringe or the like, and the needle of this syringe is pierced into the cover plate 13 corresponding to the sample loading tank 14a to inject the sample into the sample loading tank 14a. Thereafter, by operating the centrifugal device, a centrifugal force acting from the rotation center side to the outer peripheral side is generated, and by this centrifugal force, the liquid starts to flow from the upstream side to the downstream side.
  • the opening part S8 provided between the sample charging tank 14a and the first mixing tank 14f is operated by the same pressing operation as in the case of the liquid channel device 10A, so that the liquid channel 12 in this part is opened.
  • the sample is introduced into the first mixing tank 14f by centrifugal force.
  • the sample is introduced into the first mixing tank 14f, while the opening portion S9 between the first reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, and is enclosed in advance.
  • the first reagent that had been introduced is introduced into the first mixing tank 14f, and then the opening portion S10 between the second reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, so that the second sealed in advance
  • the reagent is also introduced into the first mixing tank 14f, and the sample, the first reagent, and the second reagent are mixed in the first mixing tank 14f.
  • the inflow of the sample into the first mixing tank 14 is activated by operating the closing portion T2. Can also be stopped.
  • the opening S11 between the first mixing tank 14f and the second mixing tank 14h is operated by a pressing operation to introduce the intermediate preparation liquid prepared in the first mixing tank 14f into the second mixing tank 14h.
  • the opening part S12 between the third reagent tank 14g and the second mixing tank 14h and the opening part S13 between the third reagent tank 14g and the second mixing tank 14h are actuated by pressing operation in advance.
  • the sealed third reagent and fourth reagent are also introduced into the second mixing tank 14h, and the intermediate preparation liquid, the third reagent, and the fourth reagent are mixed in the second mixing tank 14h.
  • the entire liquid flow path device 10B is supplied to the detection analysis unit, and the target component is detected and analyzed for the measurement liquid prepared in the second mixing tank.
  • the opening portion S14 can be operated to discard the measurement liquid after measurement into the waste tank 14m.
  • Such a liquid circulation device 10B also includes the opening portions S8 to S14 that make the liquid flow channel 12 from the closed state to the open state and the closing portion T2 that makes the liquid flow channel 12 from the open state to the closed state.
  • the flow of the liquid can be controlled, and as a result, detection and analysis can be performed with good accuracy in a short time.
  • the opening portions S8 to S14 and the closing portion T2 are low in cost and simple in configuration, and can be operated only by a simple pressing operation.
  • the pressure contact disk 21 in this example is provided on an arm 23 extending laterally from a rotation shaft 22 of a centrifugal device that rotates the liquid flow path device 10B, and rotates in the length direction of the arm 23 while rotating around the arm 23.
  • the liquid flow path device 10B moves from the rotation center side toward the outer peripheral side. Therefore, by moving the pressure contact disk 21 in this way while rotating the liquid flow path device 10B by the centrifugal device, the pressure contact disk 21 moves on the liquid flow path device 10B arranged in a circle from the rotation center side to the outer peripheral side. It is possible to scan in a relatively spiral shape and sequentially press the opening portions S8 to S14 and the closing portion T2 provided in the liquid channel device 10B.
  • a method of injecting a sample into the sample introduction tank 14a of the liquid flow path apparatuses 10A and 10B a method of piercing the needle of the syringe into the cover plate 13 is exemplified.
  • a sample injection hole may be formed, and the sample may be injected therefrom.
  • the sample injection hole may be covered with a protective tape and injected by piercing the syringe with the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.
  • the substrates 11A and 11B on which the liquid flow path 12 and the liquid tank are formed include, for example, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin.
  • a resin plate such as an epoxy resin, a phenol resin, an ABS resin, a polypropylene resin, or a fiber reinforced plastic, or a glass plate can be used.
  • a glass plate, a styrene resin, an acrylic resin, a polycarbonate resin, and vinyl chloride are transparent in that the state of the liquid flowing through the liquid flow path 12 can be visually observed from the side of the substrates 11A and 11B.
  • Resin, PEN resin and polyester resin are preferred.
  • a resin plate is preferable in that it is less likely to break than a glass plate and has excellent handleability.
  • the thickness of the substrates 11A and 11B is not particularly limited and may be determined according to the depth of the liquid flow path 12 to be formed, but is usually 0.5 to 7 mm.
  • the liquid flow path 12 and the liquid tank are formed in a groove shape on one surface of the substrates 11A and 11B by techniques such as photolithography, injection molding, blow molding, bonding formation, dissolution formation, cutting formation, and machining.
  • the cross-sectional shape of the liquid flow path 12 (the cross-section in the direction perpendicular to the flow), and examples thereof include a semicircular shape, a quadrangular shape, and an inverted triangular shape.
  • the width and depth of the liquid flow path 12 are not particularly limited, and may be determined according to the required liquid flow rate.
  • the small flow path 12 is preferably subjected to a surface treatment according to the type of liquid in order to facilitate the flow of the liquid.
  • a surface treatment include coating application treatment, plasma treatment, flame treatment, chemical treatment, physiological activity treatment, and antibody treatment.
  • the liquid flow path 12 may be provided with a baffle plate, a stirring plate, a protrusion, or formed with a water diverting shape, if necessary, so that the flowing liquid is in a uniform mixed state. .
  • Each liquid tank is not particularly limited in shape, and may be appropriately formed according to the volume required for each liquid tank.
  • Each dam plate is formed of a flexible resin sheet or the like, and may be disposed at a predetermined site, or may be integrally formed from the substrate 11A when forming the liquid channel 12 or the liquid tank. .
  • the first base material layer 13a constituting the surface of the cover plate 13 has a restoring force to bend and then return when a vertical load is applied from the surface side. If it is a base material having such characteristics, that is, flexibility and restoring force, it can be used as the first base material layer 13a, and its material and thickness are not particularly limited, but polyethylene terephthalate (PET), A film having a thickness of 50 to 500 ⁇ m made of polyethylene naphthalate (PEN), polycarbonate (PC), polyimide, or the like is preferable because it has flexibility and resilience appropriate for use as the first base material layer 13a. .
  • PET polyethylene terephthalate
  • PEN polyethylene naphthalate
  • PC polycarbonate
  • polyimide polyimide
  • the second base material layer 13c only needs to be easily bent by a load in the vertical direction, and more preferably does not recover. If it is the base material which has such a characteristic, it can be used as the 2nd base material layer 13c.
  • Metal foil such as aluminum foil and copper foil, paper, PET, PEN, A film having a thickness of 5 to 50 ⁇ m made of a resin such as PC or polyimide is preferable for use as the second base material layer 13c.
  • paper waterproof paper is preferable, and when using metal foil, rust-proof metal foil is preferable.
  • the strong adhesive layer 13b and the weak adhesive layer 13d can be appropriately selected from conventionally known adhesives according to the material of the first base material layer 13a and the second base material layer 13c.
  • the adhesive force (adhesive strength) of the adhesive forming the strong adhesive layer 13b needs to be stronger than the adhesive force of the adhesive forming the weak adhesive layer 13d.
  • the pressure-sensitive adhesive of the pressure-sensitive adhesive forming the strong pressure-sensitive adhesive layer 13b is equal to or lower than the pressure-sensitive adhesive of the pressure-sensitive adhesive forming the weak pressure-sensitive adhesive layer 13d, even when a pressing operation is performed in the opening portions S1 to S14, 15a and the weak adhesion layer 13d cannot be separated, and the liquid channel 12 cannot be opened.
  • the adhesive force of the adhesive forming the strong adhesive layer 13b is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layer 13d. Further, it is preferably large in the range of 0.1 to 30 N / cm.
  • the adhesive force of the adhesive forming the strong adhesive layer 13b is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layer 13d, the opening portions S1 to S14 can be operated reliably. On the other hand, it is difficult to configure these adhesive layers so that the difference in adhesive strength exceeds 30 N / cm.
  • the adhesive strength of the strong adhesive layer 13b is preferably in the range of 1 to 30 N / cm
  • the adhesive strength of the weak adhesive layer 13d is preferably in the range of 0.05 to 5 N / cm.
  • the adhesive used for the strong adhesive layer 13b and the weak adhesive layer 13d examples include acrylic, rubber-based, polyurethane-based, polyester-based, and silicon-based adhesives.
  • the strong adhesion layer 13b may be made of acrylic or rubber, and may further include a nonwoven fabric, polyester fiber, or the like as a core material.
  • the weak adhesive layer 13d is preferably made of acrylic or silicon.
  • the glass transition temperature of the resin constituting each adhesive is appropriately adjusted, or the tackifier is added to the adhesive.
  • a method of adding an additive such as a curing agent and a core material and adjusting the amount of the additive.
  • the thickness of the strong adhesive layer and the weak adhesive layer is not limited, but is usually 10 to 1000 ⁇ m.
  • adhesive strength here means the adhesive strength by peeling 180 degrees against a stainless steel plate of JIS Z 0237.
  • the spacer member 17 paper such as PET, PEN, PC, acrylic resin, epoxy resin, phenol resin, polyurethane resin, paper, or the like can be used.
  • the thickness of the spacer member 17 is not particularly limited, but if it is in the range of 50 to 2000 ⁇ m, the strong adhesion layer 13b and the second base material layer 13c are reliably separated before the opening portions S1 to S14 are operated. On the other hand, at the time of operation, the strong adhesion layer 13b and the second base material layer 13c can be reliably adhered.
  • the lid plate 13 of this part is bent by the operation of pressing the lid plate 13 of the part corresponding to the liquid tank from the outside as the liquid feeding parts P1 to P5.
  • An example of a mode in which the liquid is fed by reducing the internal volume of the liquid tank is shown.
  • the operation of pressing the bottom of the liquid tank from the outside reduces the internal volume of the liquid tank and feeds the liquid in the liquid tank.
  • the liquid feeding part in the form will be described.
  • the substrate 11B is composed of three layers: an outer layer 11e, an intermediate layer 11f stacked on the inner side, and an inner layer 11g stacked on the inner side.
  • an upper portion of a liquid tank (only the measuring tank 14c is shown in this example), a liquid flow path 12, a first convex portion 15, and a second convex portion 16 are formed.
  • a lower part of the liquid tank is formed in the intermediate layer 11f.
  • the intermediate layer 11 f constitutes the bottom of the liquid flow path 12.
  • the outer layer 11e is disposed on the outermost side of the substrate 11B and constitutes the bottom of the liquid tank.
  • the operation of pressing the bottom of the liquid tank from the outside as indicated by the arrow C ′ causes the outer layer 11e constituting the bottom of the liquid tank to bend inward, and the internal volume of the measuring tank 14c is reduced.
  • the liquid feeding part P1 ′ is activated.
  • the weir plate 18 as a backflow prevention part is formed in the inner layer 11g.
  • the dam plate 18 has a base end 18b at the boundary between the measuring tank 14c and the liquid channel 12 on the upstream side of the measuring tank 14c so that the distal end 18a of the dam plate 18 is inclined downstream. 12 is fixed to one side wall, and the tip 18a and both side ends are not fixed. Therefore, when the liquid is sent from the upstream unillustrated filtration tank to the measuring tank 14 c, the liquid can flow into the measuring tank 14 c beyond the tip 18 a of the weir plate 18.
  • the liquid feeding part P1 ′ of the measuring tank 14c is activated, the liquid in the measuring tank 14c is fed only to the downstream side by the action of the weir plate 18 and does not flow backward to the upstream side.
  • the substrate 11B of this example can be manufactured as shown in FIG. First, a sheet 11g ′ forming an inner layer 11g is prepared. In this sheet 11g ′, a portion corresponding to the liquid flow path 12 is punched in a linear shape, and a portion corresponding to the upper portion of the liquid tank is punched in a hole shape. Also, at this time, the portions that become the first convex portion 15 and the second convex portion 16 are not punched out and are left behind, and then the height of the second convex portion 16 is lower than the first convex portion 15. The height of the second convex portion is adjusted by polishing or the like.
  • the barrier plate 18 is also formed from the inner layer 11g, the portion that becomes the barrier plate 18 in the sheet 11g ′ is left without being punched.
  • a sheet 11f ′ forming the intermediate layer 11f is prepared, and in this sheet 11f ′, a portion corresponding to the lower part of each liquid tank such as the measuring tank 14c is punched out into a hole shape.
  • a sheet 11e ′ forming the outer layer 11e is prepared, and a sheet 11f ′ forming the intermediate layer 11f and a sheet 11g ′ forming the inner layer 11g are laminated and bonded to each other, whereby the substrate 11B can be manufactured.
  • each of the sheets 11e ′, 11f ′, and 11g ′ can be selected from the materials of the substrate 11A exemplified in the first embodiment.
  • the sheet 11e ′ constitutes the bottom of the liquid tank and is pressed from the outside when the liquid feeding section is operated, it is necessary to use a flexible sheet.
  • the thickness of the sheet 11g ′ corresponds to the depth of the liquid flow path 12 to be formed, and the sum of the thicknesses of the sheet 11g ′ and the sheet 11f ′ corresponds to the total depth of the liquid tank. Therefore, the thicknesses of the sheet 11f ′ and the sheet 11g ′ are determined in consideration of the depth required for the liquid tank and the liquid channel 12.
  • the suitable depth of the liquid flow path 12 is the same range as 1st Embodiment.
  • the sheet 11e ′ since the sheet 11e ′ needs to bend when the liquid feeding section is operated as described above, specifically, it is preferably 20 to 300 ⁇ m although it depends on the material.
  • the substrate 11B may be composed of a laminate of a sheet 11e 'and a sheet 11g', and may be composed of two layers, an outer layer 11e and an inner layer 11g. In this case, the depth of the liquid channel 12 and the liquid tank are the same.
  • the outer layer 11e of the substrate 11B corresponding to the weighing tank 14c that is, The bottom of the measuring tank 14c may be bulged outward.
  • the internal volume of the measuring tank 14c can be made smaller, and as a result, the liquid in the measuring tank 14c can be made more effective. Liquid can be fed.
  • the liquid flow path device 10C of the third embodiment can be obtained. That is, also in this example, as shown in FIGS. 10A and 10B, the strong adhesive layer 13b and the second base material layer 13c are separated from each other in the cover plate 13 corresponding to the weighing tank 14c. Instead, the spacer member 17 is interposed, the spacer member 17 and the strong adhesion layer 13b adhere to each other, and the layers are densely configured. Therefore, when the bottom of the measuring tank 14c is pressed and bent from the outside and the liquid feeding part P1 'is operated, the internal volume of the measuring tank 14c is reduced, and the action as the liquid feeding part is exhibited.
  • the spacer member 17 is not interposed, and the interlayer is not dense, the bottom of the measuring tank 14c is pressed from the outside.
  • the second base material layer 13c and the weak adhesive layer 13d are bent outward due to the internal pressure of the measuring tank 14c, the internal volume of the measuring tank 14c is not reduced, and the function as the liquid feeding unit may not be exhibited. is there.
  • the substrate 11B is composed of three layers of the outer layer 11e, the intermediate layer 11f and the inner layer 11g, or two layers of the outer layer 11e and the inner layer 11g.
  • the barrier plate 18, the first convex portion 15 and the second convex portion 16 are formed by punching out the sheet 11f ′ constituting the intermediate layer 11f and the sheet 11g ′ constituting the inner layer 11g. Therefore, for a substrate made of a single flat plate, a method for forming a liquid tank or a liquid flow path by, for example, photolithography, a method for forming a substrate in which a liquid tank or a liquid flow path is formed by injection molding, etc. Compared with a low manufacturing cost, a liquid tank etc. can be formed easily and mass production is also possible.
  • the liquid flow path apparatuses 10A, 10B, and 10C in which the liquid flow path 12 is formed only on one surface of the substrates 11A and 11B are exemplified.
  • the substrates 11A and 11B are exemplified.
  • the liquid channel 12 may be formed on both sides of the liquid crystal.
  • limiting in the form of the communication hole which can be opened and closed provided in each liquid tank The form which can open and close a communication hole by inserting and extracting a fitting type cap to the communication hole formed in the cover plate.
  • an opening portion and a closing portion having the same configuration as the opening portions S1 to S7 and the closing portion T1 provided in the liquid channel 12 may be provided.
  • a liquid feeding section that operates by pressing the bottom of the liquid tank may be provided in the liquid tank.
  • a liquid feeding section that operates by pressing the cover plate 13 corresponding to the liquid tank may be provided in the liquid tank.
  • the liquid flow path device provided with the closing part and the opening part is given as an example, the first base material layer 13a, the strong adhesive layer 13b, the second base material layer 13c, the weak base material are used as the cover plate 13.
  • the lid plate 13 does not need to be composed of a plurality of layers in this way for the operation of the liquid feeding unit, and is a single layer lid plate. It may be.
  • the liquid flow path 12, a part of liquid tank, or these Both are heated to expand the air in the liquid flow path 12 and the liquid tank, or an oxygen absorbent (such as iron powder that easily oxidizes) is sealed in a part of the liquid flow path 12, A method of reducing the pressure in the liquid flow path 12 by absorbing oxygen and moving and circulating the liquid may be used in combination.
  • a method of injecting the sample into the sample loading tank 14a a method of piercing the needle of the syringe into the cover plate 13 is exemplified.
  • a sample injection hole is formed in the cover plate 13 in advance.
  • the sample may be injected therefrom.
  • the sample injection hole may be covered with a protective tape and injected by piercing the syringe with the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.
  • the communicating hole provided in each liquid tank is sealed before use of liquid flow-path apparatus 11A, 11B, and is opened at the time of use. Therefore, an opening portion having the same configuration as the opening portions S1 to S14 provided in the liquid channel 12 may be provided before the communication hole.
  • the liquid channel device 10A uses gravity and the liquid channel device 10B uses centrifugal force.
  • the method is not limited thereto. For example, a part of the liquid channel 12 is pressurized, a part of the liquid channel 12 is heated to expand the air in the liquid channel 12, or an oxygen absorbent (oxidation) is added to a part of the liquid channel 12.
  • the measuring tank 14 may be pressurized, heated, or depressurized instead of a part of the liquid channel 12, or depending on the case, both the liquid channel 12 and the measuring tank 14 may be pressurized, heated, or depressurized. May be.
  • sample and reagent through which the liquid flow path devices 10A and 10B are circulated there are no particular limitations on the sample and reagent through which the liquid flow path devices 10A and 10B are circulated, and a sample and a reagent that have been conventionally used in the medical field, the environmental field, and the like can be used in appropriate combination.
  • samples eg, blood (whole blood), plasma, serum, buffy coat, urine, feces, saliva, sputum, etc.
  • viruses eg. blood (whole blood), plasma, serum, buffy coat, urine, feces, saliva, sputum, etc.
  • viruses eg. DNA or RNA isolated from these may be used, and a sample obtained by subjecting them to any pretreatment or dilution may be used.
  • a reagent when an antigen present in a sample is analyzed, a reagent containing an antibody against the antigen is preferable.
  • conventionally known optical means, electrical means, and the like can be appropriately employed as the detection / analysis unit for the measurement liquid prepared by the liquid flow path apparatuses 10A and 10B.
  • FIG. 14 is a plan perspective view schematically showing an embodiment of the liquid channel device of the fourth embodiment
  • FIG. 15 is a plan perspective diagram showing an enlarged part of the liquid channel device of FIG. 14, and FIG. It is sectional drawing which follows the II 'line
  • the liquid channel device 110 includes a groove-like liquid channel 112 through which a liquid consisting of at least one of a sample and a reagent flows on one side of a rectangular substrate 111 made of a flat plate, and an end portion or a middle of the liquid channel 112.
  • a plurality of (9 in this example) liquid tanks (114a to 114i) in which liquid is accumulated are formed, and a cover plate 113 is laminated on the flow path forming surface 112a on the side where the liquid flow path 112 of the substrate 111 is formed.
  • a cover plate 113 is laminated on the flow path forming surface 112a on the side where the liquid flow path 112 of the substrate 111 is formed.
  • a sample loading tank 114a in which the loaded sample is stored is provided, and downstream of the sample loading tank 114a, the sample flowing from the sample loading tank 114a is provided.
  • a filtration tank 114b containing a filter (not shown) to be filtered is provided.
  • a measuring tank 114c for measuring a certain amount of the filtered sample is provided downstream of the filtering tank 114b.
  • the measuring tank 114c of this example is provided with an overflow portion comprising an overflow channel 112d and a waste liquid tank 114d provided downstream thereof. Therefore, the sample exceeding a certain amount in the measuring tank 114c overflows and flows through the overflow channel 112d and flows into the waste liquid tank 114d. As a result, a certain amount of sample can be measured in the measuring tank 114c.
  • a first mixing tank 114f Downstream of the measuring tank 114c, a first mixing tank 114f is provided in which the sample weighed in the measuring tank 114c and the liquid first reagent sealed in a predetermined amount in the first reagent tank 114e are mixed. Downstream of the first mixing tank 114f is a second mixing in which the intermediate preparation liquid prepared in the first mixing tank 114f and the liquid second reagent sealed in a predetermined amount in the second reagent tank 114g are mixed.
  • a tank 114h is provided.
  • a measuring tank 114i is provided downstream of the second mixing tank 114h (the end on the downstream side of the liquid channel 112), and the measurement liquid prepared in the second mixing tank 114h is stored here, and is not shown.
  • the detection analysis unit detects and analyzes various components. Each liquid tank is provided with a communication hole (not shown) that can be opened and closed and communicates with the atmosphere as needed.
  • the substrate 111 of the liquid channel device 110 includes three layers: an outer layer 111a, an intermediate layer 111b stacked on the inner side, and an inner layer 111c stacked on the inner side.
  • an upper portion (portion on the lid 113 side of the liquid tank) of the liquid tank (only the sample charging tank 114a and the filtration tank 114b are shown in FIG. 3) and the liquid channel 112 are formed.
  • the intermediate layer 111b a lower part of the liquid tank (a part other than the upper part and on the bottom side of the liquid tank) is formed.
  • the surface on the inner layer 111 c side forms the bottom portion 112 b of the liquid channel 112.
  • the outer layer 111a is disposed on the outermost side of the substrate 111, and the surface on the intermediate layer 111b side forms the bottom of the liquid tank.
  • the liquid flow path device 110 includes opening portions S11 to S17 that make a part of the liquid flow path 112 open from the closed state, and a closing portion T11 that changes the open state to the closed state.
  • the opening sections S11 to S17 include the first mixing tank 114a and the filtering tank 114b, the filtering tank 114b and the measuring tank 114c, the measuring tank 114c and the first mixing tank 114f, Between the tank 114f and the second mixing tank 114h, between the first reagent tank 114e and the first mixing tank 114f, between the second reagent tank 114g and the second mixing tank 114h, and between the second mixing tank 114h and the measuring tank One is provided in each liquid flow path 112 between 114i.
  • the closing part T11 is provided downstream of the opening part S12 in the liquid channel 112 between the filtration tank 114b and the measuring tank 114c.
  • the opening portions S11 to S17 are arranged in the liquid channel 112 so as to block a part of the liquid channel 112 and seal the flow of the liquid, as described with reference to S11 and S12 in FIG. In addition, it is formed by a resin plug 115 that closes this portion.
  • the plug 115 is made of a resin that can be plastically deformed, and is plastically deformed by pressing the lid plate 113 where the plug 115 is disposed from the outside, so that the liquid channel 112 is opened from the closed state.
  • the plug body 115 constituting the opening portion S11 is pressed from the outside of the cover plate 113 as indicated by an arrow A to apply a load.
  • the cover plate 113 bends as shown in FIG. 17A, and the plug 115 that is in contact with the cover plate 113 is crushed and plastically deformed flat. Then, when the load is removed thereafter, as shown in FIG. 17B, the cover plate 113 is restored to its original state by its restoring force, but the plug body 115 remains flat and does not restore. As a result, the plug body 115 and the cover plate 113 are newly separated from each other so that liquid can flow therethrough. In this way, in the opening portions S11 to S17, the stopper 115 is pressed from the outside of the lid plate 113 and a load is applied, and then the stopper 115 and the lid plate that were originally in close contact with each other by a pressing operation to remove the load. As a result, the liquid flow path 112 in this portion is changed from the closed state to the open state.
  • the closing portion T11 of the liquid flow path device 110 includes a sealing material supply tank 116 that is branched from the liquid flow path 112 and formed on the substrate 111, and the sealing material supply tank. 116 and a paste-like sealing material 117 filled in 116.
  • the sealing material 117 is pressed by pressing the lid plate 113 corresponding to the sealing material supply tank 116 from the outside.
  • the liquid channel 112 is pushed through a supply channel 118 that connects the liquid channel 112 and the liquid channel 112, and the portion is changed from the open state to the closed state.
  • the liquid channel device 110 As a specific method for preparing a measurement liquid using such a liquid channel device 110, first, the liquid channel device 110 is positioned so that the sample introduction tank 114a side is positioned above and the measurement tank 114i side is positioned below. Therefore, the liquid is likely to flow from the upstream side to the downstream side by gravity. Next, the sample is sampled in a syringe or the like, and the needle of this syringe is pierced into the cover plate 113 corresponding to the sample loading tank 114a, and the sample is injected into the sample loading tank 114a.
  • the opening portion S11 provided between the sample introduction tank 114a and the filtration tank 114b, that is, the stopper 115 is pressed from the outside of the cover plate 113 to be plastically deformed, and the liquid flow path 112 in this portion is opened.
  • the sample is introduced to the filtration tank 114b by gravity.
  • the pressing operation may be performed manually by an operator pressing with a finger, or a predetermined position may be pressed using a pressing device in which the pressing position is programmed in advance as XY coordinates. Good.
  • the plug body 115 of the opening portion S12 provided between the filtration tank 114b and the measuring tank 114c is also plastically deformed, and the liquid flow path 112 of this portion is made to be the same.
  • the open state is established, and the sample is introduced into the measuring tank 114c by gravity.
  • the closing portion T11 provided between the filtration tank 114b and the measuring tank 114c is operated, and the liquid flow path 112 of this portion is operated. Is closed.
  • the cover plate 113 corresponding to the sealing material supply tank 116 is pressed as indicated by an arrow B to apply a load, and the sealing material 117 filled in the sealing material supply tank 116 is liquid.
  • the liquid channel 112 is pushed out to the flow channel 112 and the liquid flow channel 112 in this portion is closed.
  • the opening portion S13 provided downstream of the measuring tank 114c is operated, and the sample measured in the measuring tank 114c. Is introduced into the first mixing tank 114f.
  • the stopper is similarly plastically deformed in the opening portion S14 between the first reagent tank 114e and the first mixing tank 114f by the first reagent. Is introduced into the first mixing tank 114f, and the sample and the first reagent are mixed in the first mixing tank 114f to prepare an intermediate preparation solution. Next, also in the opening part S15 between the first mixing tank 114f and the second mixing tank 114h, the intermediate preparation liquid prepared in the first mixing tank 114f by plastically deforming the stopper in the same manner is used as the second mixing tank 114h. To introduce.
  • the opening reagent S16 between the second reagent tank 114g and the second mixing tank 114h is also plastically deformed to introduce the second reagent into the second mixing tank 114h. Then, the intermediate preparation solution and the second reagent are mixed in the second mixing tank 114h to prepare a measurement solution.
  • the stopper is similarly plastically deformed, and the measurement liquid prepared in the second mixing tank 114h is introduced into the measurement tank 114i. And after introduce
  • the liquid flow path 112 includes the opening portions S11 to S17 that make the liquid flow path 112 from the closed state to the open state and the closing portion T11 that makes the liquid flow path 112 from the open state to the closed state.
  • the flow of the liquid inside can be controlled, and as a result, highly accurate detection and analysis can be performed in a short time.
  • a closing portion T11 is provided upstream of the measuring tank 114c, and an opening portion S13 is provided downstream. Therefore, the sample can be accurately weighed in the weighing tank 114c in a short time and introduced into the first mixing tank 114f.
  • the opening portion S13 is not provided downstream of the measuring tank 114c and the liquid flow path 112 in this portion is always open, the sample is continuously transferred from the measuring tank 114c even during measurement. The sample cannot be stored in a certain amount, and measurement itself is impossible. Further, when the closing portion T11 is not provided upstream of the measuring tank 114c, after the entire amount of the sample that has passed through the filtration tank 114b has completely flowed into the measuring tank 114c, the measuring tank 114c and the first mixing tank 114f. It is necessary to operate the opening portion S13 between and introduce the weighed sample into the first mixing tank 114f.
  • the sample is a liquid having a viscosity such as blood
  • the closing portion T11 is provided on the upstream side of the measuring tank 114c, even if the entire amount of the sample that has passed through the filtration tank 114b does not completely flow into the measuring tank 114c, the measuring tank When the sample starts to overflow in 114c, the closing portion T11 is operated to stop further inflow of the sample into the measuring tank 114c, and accurate measurement can be performed in a short time.
  • an opening portion S15 is provided between the first mixing vessel 114f and the second mixing vessel 114h
  • an opening portion S17 is provided between the second mixing vessel 114h and the measurement vessel 114i. Therefore, in the first mixing tank 114f and the second mixing tank 114h, after the target mixing and reaction have sufficiently progressed, the opening portions S15 and S17 are opened, and the intermediate preparation liquid and the measurement liquid are respectively supplied to the second mixing tank. 114h and the measuring tank 114i. Therefore, it is possible to prevent a decrease in detection and analysis accuracy due to insufficient mixing and reaction.
  • the opening portions S14 and S16 are provided between the first reagent tank 114e and the first mixing tank 114f and between the second reagent tank 114g and the second mixing tank 114h, it is desirable. These are opened at the point of time, and the first reagent and the second reagent previously sealed in the first reagent tank 114e and the second reagent tank 114g, respectively, are allowed to flow into the first mixing tank 114f and the second mixing tank 114h. Can do. If the opening portions S14 and S16 are not provided, the first reagent and the second reagent may start to flow downstream when the liquid channel device 110 is stored.
  • the opening portions S11 to S17 and the closing portion T11 of the liquid channel device 110 of this example have a simple configuration and can be formed at low cost. Therefore, the liquid channel device 110 can be a disposable type. In addition, the opening and closing operations can be performed only by a simple pressing operation, and the operability is excellent.
  • Such a liquid flow path device 110 includes a first step of forming a liquid flow path 112, a liquid tank, a sealing material supply tank 116, and a supply path 118 on a substrate 111, and a predetermined liquid flow path 112 formed.
  • the second step is different from the above step, and the formed sealing material supply tank 116 is filled with the sealing material.
  • the manufacturing process of the liquid flow path device 110 will be described with reference to FIG. 20 schematically showing the manufacturing process of the liquid flow path device 110.
  • a wound material 111a ′ is prepared.
  • the sheet 111c ′ is continuously supplied from the wound material 120 of the sheet 111c ′ constituting the inner layer 111c, and a portion corresponding to the liquid flow path 112 is punched out linearly by a punching machine 123a.
  • the liquid flow path device 110 includes a supply path 118 formed by branching from a part of the liquid flow path 112 and a sealing material supply tank 116. Therefore, in the sheet 111c ′, the supply path 118 is provided. The portions corresponding to the sealing material supply tank 116 are also punched into shapes corresponding thereto.
  • the sheet 111b ′ is continuously supplied from the wound material 121 of the sheet 111b ′ constituting the intermediate layer 111b, and a portion corresponding to the lower part of each liquid tank such as the measuring tank 114c is opened by the punching machine 123b. Punch into a shape.
  • the sheet 111a ′ is continuously supplied from the wound material 122 of the sheet 111a ′ constituting the outer layer 111a.
  • substrate 111 is manufactured by laminating
  • each of the sheets 111a ′, 111b ′, and 111c ′ is preferably bonded by an adhesive supplied from an unillustrated adhesive supply device, but depending on the material of each of the sheets 111a ′, 111b ′, and 111c ′. May be bonded together by heat fusion or the like.
  • the sheets 111a ′, 111b ′, and 111c ′ are supplied from the rolls 120, 121, and 122, and the sheets 111b ′ and 111c ′ are each punched into a predetermined shape.
  • a process of sequentially laminating and adhering sheets 111a ′, 111b ′, and 111c ′ it is possible to continuously produce a large number of substrates 111 in which the liquid channel 112 and the liquid tank are formed.
  • Such a method is a method in which a liquid tank or a liquid channel is formed on each substrate made of a single flat plate by, for example, photolithography, cutting, or the like.
  • the manufacturing cost is low, it is simple, mass production is possible, and it is industrially suitable.
  • the method of punching the sheets 111b ′ and 111c ′ into a predetermined shape to form the liquid flow path 112 and the liquid tank is low in cost and excellent in productivity, but other methods (laser processing, knife, etc. are used).
  • the sheet 111b ′, 111c ′ may be opened in a predetermined shape by cutting, thermal processing, etc., to form the liquid channel 112, the liquid tank, and the like.
  • the substrate 111 of the liquid channel device 110 is configured by three layers of the outer layer 111a, the intermediate layer 111b, and the inner layer 111c, but is configured by two layers of the outer layer 111a and the inner layer 111c. May be.
  • the liquid flow path 112, the liquid tank, the supply path 118, and the sealing material supply tank 116 are formed in the sheet 111c ′ forming the inner layer 111c.
  • the formed liquid flow path 112 and the depth of the liquid tank are the same.
  • the sealing material supply tank 116 is formed on the sheet 111c ′ forming the inner layer 11c.
  • the upper part of the sealing material supply tank forms the inner layer 111c.
  • the sealing material supply tank may be formed to have the same depth as the liquid tank by forming the sheet 111c ′ and forming the lower part of the sheet 111b ′ forming the intermediate layer 111b.
  • the materials of the sheets 111a ′, 111b ′, 111c ′ constituting the outer layer 111a, the intermediate layer 111b, and the inner layer 111c of the substrate 111 are styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, polyester resin, epoxy Examples thereof include resins such as resins, phenol resins, ABS resins, polypropylene resins, and fiber reinforced plastics. Among these, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin are preferable because they are transparent and the state of the liquid flowing through the liquid flow path 12 can be visually observed.
  • the resin is exemplified as the sheet material in this way. is doing.
  • the manufacturing method For example, when it is required to stably support the liquid flow path device, a transparent material other than a resin such as glass is used for the substrate, and this is subjected to a cutting process. It is also possible to form a liquid channel, a liquid tank, and the like by applying the above method.
  • each of the sheets 111a ′, 111b ′, and 111c ′ can be set as appropriate, but in the case of the liquid channel device 110 shown in the drawing, the thickness of the sheet 111c ′ that forms the inner layer 111c is the liquid channel 112 that is formed.
  • the sum of the thicknesses of the sheet 111c ′ forming the inner layer 111c and the sheet 111b ′ forming the intermediate layer 111b corresponds to the total depth of the liquid tank. Therefore, the thickness of the sheet 111b ′ and the sheet 111c ′ is determined in consideration of the depth required for the liquid tank and the liquid channel 112.
  • the thickness of the sheet 111b ′ is preferably 25 to 500 ⁇ m, and the thickness of the sheet 111c ′ is preferably 10 to 300 ⁇ m. Further, in this example (in the case where the cover plate 113 is pressed), the thickness of the sheet 111a ′ is preferably 50 ⁇ m or more, and more preferably 100 to 1000 ⁇ m as the support layer of the liquid flow path device 10. Works well. There are no particular limitations on the width of the liquid flow path 112, the volume and shape of each liquid tank and the sealing material supply tank 116, and can be set as appropriate.
  • the width of the liquid channel 112 is preferably 25 to 2000 ⁇ m, more preferably 500 to 2000 ⁇ m, and the volume of the liquid tank is preferably 50 to 50000 ⁇ l, more preferably 100 to 1000 ⁇ l.
  • the waste liquid tank 114d and the like do not have a particularly suitable volume, and can be freely designed according to the function of each liquid tank.
  • the thickness of the sheet 11c ′ forming the inner layer 11c corresponds to the depth of the sealing material supply tank 16 and the supply path 18 to be formed. .
  • a second step of forming the plug body 115 is performed.
  • a plug forming material for forming the plug 115 is formed at a predetermined position in the liquid flow path 112 of the substrate 111 that is continuously supplied by a printing machine (for example, a screen printing machine), a dispenser, and a coater.
  • the stopper 115 is formed by a coating method using a coating device 124a such as a roll coater or a knife coater.
  • the second step a part of the liquid channel 112 formed on the substrate 111 in the first step, that is, each of the opening portions S11 to S17 is provided.
  • the stopper 115 is formed at the position, and the sealing material 117 is filled into the sealing material supply tank 116 formed on the substrate 111 in the first step.
  • the plug body 115 is formed by printing a plug body forming material for forming the plug body 115 at a predetermined position in the liquid flow path 112 of the substrate 111 supplied continuously by a printing machine, a dispenser, a coater (roll coater, knife coater, etc.). ) Or the like.
  • the plug-forming material is not limited as long as it does not interact with the liquid flowing through the liquid flow path 112 and can reliably close the liquid in the closed state and can be plastically deformed by pressing.
  • a resin composition containing a plastic component, a filler, and a solvent is used.
  • the viscosity of the resin composition is preferably 30 to 500 dPa ⁇ s.
  • the viscosity of the resin composition is 50 to 500 dPa ⁇ s. Is preferred.
  • the resin component preferably has a glass transition temperature of ⁇ 10 ° C.
  • the resin include epoxy resins, polyester resins, chlorine resins, acrylic resins, ester resins such as phthalic acid, and the like, and one or more resins can be used.
  • the plastic component a plasticizer having a glass transition temperature of 30 ° C. or less is preferably used.
  • the plasticizer include thermoplastic resins having a low melting point such as hard resin resins, epoxy resins, and polyester resins. One or more plasticizers can be used.
  • the filler is blended to adjust the viscosity of the plug-forming material and the plug-forming property, and examples thereof include precipitated barium sulfate, talc, acicular silicon oxide, and hollow beads.
  • the above can be used.
  • plugs formed from resin compositions containing hollow beads made of glass, resin, etc.
  • a solvent is mix
  • a suitable organic solvent is used.
  • the plug body forming material can be applied by printing or the like without containing a solvent, it may not contain a solvent, and preferably contains no solvent.
  • the plug body 115 can be continuously and efficiently formed at a predetermined position.
  • the sealing material supply tank 116 constituting the closing portion T11 is filled with the paste-like sealing material 117 by the coating device 124b.
  • the sealing material 117 can be continuously and efficiently filled into a predetermined position.
  • the paste-like sealing material 117 may be any material that does not interact with the liquid flowing through the liquid flow path 112 and can be pushed out to close the liquid flow path 112.
  • a resin component, a plastic component, and the like For example, a resin composition containing a filler and having a viscosity of 30 to 500 dPa ⁇ s is preferably used.
  • the resin component preferably has a glass transition temperature of ⁇ 40 ° C. or less and a mass average molecular weight of 50,000 from the viewpoints of coating properties (printability, dispensing properties, etc.), fluidity, sealing ability and stability.
  • the following resins are preferably used.
  • the resin include epoxy resins, polyester resins, chlorine resins, acrylic resins, ester resins such as phthalic acid, and the like, and one or more resins can be used.
  • a plasticizer having a glass transition temperature of 30 ° C. or less is preferably used.
  • plasticizer examples include thermoplastic resins having a low melting point such as hard resin resins, epoxy resins, and polyester resins.
  • One or more plasticizers can be used.
  • the filler is blended to adjust the viscosity of the sealing material 117 and to make the liquid channel 112 easy to close when the sealing material 117 is pushed out to the liquid channel 112.
  • Fiber pieces, extender pigments, thixotropic imparting agents, and the like can be used.
  • fumed silica such as Aerosil (trade name, manufactured by Nippon Aerosil Co., Ltd.), precipitated barium sulfate, and talc are preferably used.
  • each step such as a curing step is performed.
  • a sheet 113 ′ constituting the cover plate 113 is laminated on the flow path forming surface 112 a of the substrate 111 and bonded.
  • the substrate 111 and the sheet 113 ′ are preferably bonded by an adhesive supplied from an adhesive supply device (not shown).
  • the substrates 111 and the sheet 113 ′ are bonded together by heat fusion or the like. May be. Thereby, the continuous body with which the several liquid flow-path apparatus 110 continued continuously can be manufactured.
  • the continuum of the liquid channel device 110 manufactured in this way may be wound up as shown in FIG. 20 to be in the state of a wound product 126 or in a folded state. Moreover, you may be set as the single wafer type cut
  • a step of forming a line such as a perforation or a concave line between the liquid channel devices 110 may be performed. Thereby, it becomes easy to bend between each liquid flow-path apparatus 110, and can make it easy to fold the continuous body of the liquid flow-path apparatus 110. FIG. Moreover, it can be made easy to separate into a single wafer type.
  • cover plate 113 bends when it is pressed and a load is applied as indicated by an arrow A in FIG. 17A, and when the load is removed thereafter, the cover plate 113 is restored to its original state by its restoring force. Is. If it is such, there is no restriction
  • a material for example, a styrene resin, an acrylic resin, a polycarbonate resin, a vinyl chloride resin, a PEN resin, a polyester resin, an epoxy resin And resins such as phenol resin, ABS resin, polypropylene resin, and fiber reinforced plastic.
  • the thickness of the lid plate 113 is preferably about 30 to 500 ⁇ m.
  • a release agent containing a silicone component or the like in the portion 113a in contact with the plug body 115 in the lid plate 113 is preferable to perform a peeling treatment in advance, for example, by coating.
  • a peeling treatment in advance, for example, by coating.
  • an adhesive is applied in advance to the bottom 112b of the liquid flow path 112 at a portion in contact with the plug body 115, for example. If this is done, the plug body 115 is securely bonded to this portion, so that the plug body 115 is more smoothly separated from the lid plate 113 side as shown in FIG. Can do.
  • the plug body 115 constituting the opening portions S11 to S17 is plastically deformed by an operation of pressing from the outside of the cover plate 113, so that the liquid channel 112 is formed. It illustrated about what was made into an open state. However, it may be configured to be plastically deformed by an operation of pressing the bottom 112b of the liquid flow path 112 where the plug 115 is disposed from the outside. In that case, the outer layer 111a and the intermediate layer 111b constituting the substrate 111 bend when pressed and a load is applied, and return to the original state by the restoring force when the load is removed thereafter. It is necessary to be configured to restore.
  • the thickness of the sheet 111a ′ is preferably 10 to 300 ⁇ m, more preferably 15 to 200 ⁇ m.
  • the portion 113 a in contact with the plug body 115 in the lid plate 113 is subjected to an adhesive treatment, and the portion in contact with the plug body 115 in the bottom portion 112 b of the liquid channel 112 is subjected to a peeling process.
  • the sealing material 117 is pushed out by pressing the lid plate 113 corresponding to the sealing material supply tank 116 from the outside, and the liquid flow An example of closing the path 112 was illustrated.
  • the sealing material 117 may be pushed out by pressing the bottom 116a of the sealing material supply tank 116 from the outside. Even in that case, the outer layer 111a and the intermediate layer 111b constituting the substrate 111 need to be configured to bend when pressed and a load is applied.
  • FIG. 21 is a plan perspective view schematically showing an embodiment of the liquid channel device 110A of the fifth embodiment
  • FIG. 22 is a plan perspective diagram in which a part of the liquid channel device 110A of FIG. 21 is enlarged
  • FIG. FIG. 23 is a cross-sectional view taken along the line II ′ of FIG.
  • a rectangular substrate 111A made of a flat plate and a cover plate 113A are laminated on a channel forming surface 112a on the side where the liquid channel 112 of the substrate 111A is formed.
  • the other configuration is the same as that of the fourth embodiment, and is omitted.
  • the substrate 111A of the liquid channel device 110A is composed of a plurality of layers as shown in FIG. Specifically, it is composed of three layers: an outer layer 111a, an intermediate layer 111b stacked on the inner side, and an inner layer 111c stacked on the inner side.
  • an upper portion (portion on the lid 113A side of the liquid tank) of the liquid tank (only the sample charging tank 114a and the filtration tank 114b are shown in FIG. 23) and the liquid channel 112 are formed.
  • the intermediate layer 111b a lower part of the liquid tank (a part other than the upper part and on the bottom side of the liquid tank) is formed.
  • the surface on the inner layer 111 c side forms the bottom portion 112 b of the liquid channel 112.
  • the outer layer 111a is disposed on the outermost side of the substrate 111A, and the surface on the intermediate layer 111b side forms the bottom of the liquid tank.
  • the cover plate 113A is also composed of a plurality of layers. Specifically, it is composed of two layers, an outer layer 113a and an inner layer 113b laminated inside.
  • the liquid channel device 110A includes the opening portions S11 to S17 that change a part of the liquid channel 112 from the closed state to the open state, and the closing portion T11 that changes the open state to the closed state. And have.
  • the configurations of the opening portions S11 to S17 and the closing portion T11 are the same as those in the fourth embodiment, and are omitted here.
  • the opening portions S11 to S17 are arranged in the liquid channel 112 so as to block a part of the liquid channel 112, as illustrated by exemplifying S11 and S12 in FIG. There is provided a resin plug 115 that seals the flow and closes this portion.
  • a recess 151 that can accommodate the plug body 115 is formed on the inner surface of the cover plate 113A at a position facing the plug body 115.
  • the inner layer 113b of the cover plate 113A is opened by punching or the like, and a recess 151 is formed.
  • the plug body 115 in this example has a portion (bottom portion) in contact with the bottom portion 112b of the liquid flow path 112 in the closed state. 112b is fixed.
  • the height of the plug body 115 is formed slightly higher than the height of the liquid channel 112, and the top portion 115 b side is arranged in a state of being slightly liquid-tightly inserted into the recess 151.
  • a strong adhesive layer 116a having a larger adhesive force than the weak adhesive layer 115a is formed at a position facing the top 115b of the plug body 115.
  • the stopper 115 is operated by pressing the lid plate 113A or the bottom 112b of the liquid flow channel 112 from the outside in the part where the opening parts S11 and S12 are provided (the part corresponding to the stopper 115 or the recess 151). Then, the liquid channel 112 is moved from the liquid channel 112 into the recess 151 to change the liquid channel 112 from the closed state to the open state.
  • FIG. 25A and FIG. 25B by taking the opening portion S11 as an example, when a load is applied by pressing the cover plate 113A from the outside as indicated by an arrow A, the cover is closed as shown in FIG. 25A.
  • the plate 113A bends, and the strong adhesive layer 116a of the recess 151 and the top 115b of the plug body 115 come into contact with each other and adhere to each other.
  • the cover plate 113A is restored to its original state by its restoring force, and accordingly, the plug sticking to the recess 151 by the action of the strong adhesive layer 116a.
  • the plug 115 is separated from the bottom 112 b of the liquid channel 112 and is stored in the recess 151. As a result, the plug 115 is separated from the bottom 112b of the liquid channel 112, and the liquid can flow therethrough.
  • the plug body 115 is moved by pressing the lid plate 113A where the opening portions S11 to S17 are provided from the outside to apply a load and then removing the load.
  • the liquid channel 112 moves from the liquid channel 112 into the recess 151, and as a result, the liquid channel 112 in this portion changes from the closed state to the open state.
  • the load is applied by pressing the cover plate 113A from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the opening portion S11 is provided is from the outside, that is, the substrate 111A.
  • the stopper 115 may be similarly moved from the liquid flow path 112 into the recess 151 by an operation of pressing from the outside.
  • the weak adhesive layer 115a remains on the bottom 112b side of the liquid channel 112, but may remain adhered to the plug 115 side after the liquid channel 112 is opened. good.
  • the closing portion T11 of the liquid channel device 110A includes a resin plug 150, and the plug 150 is in a recess 152 formed on the inner surface of the cover plate 113A. It is stored in. Specifically, in this example, the inner layer 113b of the cover plate 113A is opened by punching or the like to form a recess 152, and the plug 150 is disposed therein. In the opened state, the plug body 150 of this example is housed in the recess 152 with its top 117b sticking to the recess 152 by the weak adhesive layer 117a. Further, the height of the plug 150 is formed slightly higher than the height of the liquid flow path 112.
  • the top portion 117b side of the liquid flow passage 112 is placed in the recess 152. It comes to be in a state where it is closely inserted slightly.
  • a strong adhesion layer 118 a is formed at a position facing the bottom of the plug 150 at the bottom 112 b of the liquid channel 112. Then, the plug 150 is recessed by an operation of pressing the lid plate 113A or the bottom 112b of the liquid channel 112 from the outside of the portion where the closing portion T11 is provided (portion corresponding to the plug 150 or the recess 152).
  • the liquid flow path 112 is moved from the inside to the liquid flow path 112, and the liquid flow path 112 is changed from the open state to the closed state.
  • the plug 150 does not follow it and remains adhered to the liquid flow path 112. Therefore, the liquid flow path 112 is blocked by the plug 150, and the liquid is in this portion. Can not be distributed.
  • the plug body 150 is in a state where the top portion 117b side is slightly fitted in the recess 152 in a liquid-tight manner.
  • the plug 150 is removed from the inside of the recess 152 by pressing the cover plate 113A of the portion where the closing portion T11 is provided from the outside and applying a load, and then removing the load.
  • the liquid flow path 112 moves to the liquid flow path 112, and as a result, the liquid flow path 112 in this portion changes from the open state to the closed state.
  • the load is applied by pressing the cover plate 113A from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the closing portion T11 is provided is from the outside, that is, the substrate 111A.
  • the stopper 150 may be similarly moved to the liquid channel 112 by an operation of pressing from the outside.
  • the weak adhesive layer 117a remains on the concave portion 152 side in FIGS. 26A and 26B, but may be in a state of sticking to the plug 150 side.
  • the liquid channel device 110A is positioned so that the sample introduction tank 114a side is positioned above and the measurement tank 114i side is positioned below. Therefore, the liquid is likely to flow from the upstream side to the downstream side by gravity.
  • the sample is sampled in a syringe or the like, and the needle of this syringe is pierced into the cover plate 113A corresponding to the sample loading tank 114a, and the sample is injected into the sample loading tank 114a.
  • the opening portion S11 provided between the sample introduction tank 114a and the filtration tank 114b after applying the above-described pressing operation, that is, pressing the bottom of the lid plate 113A or the liquid flow path 112 from the outside. Then, the removal operation is performed, the stopper 15 is moved into the recess 151, the liquid flow path 112 in this portion is opened, and the sample is introduced to the filtration tank 114b by gravity.
  • the pressing operation may be performed manually by an operator pressing with a finger, or a predetermined position may be pressed using a pressing device in which the pressing position is programmed in advance as XY coordinates. Good.
  • the stopper 115 is also moved into the recess 151 in the same way for the opening portion S12 provided between the filtration tank 114b and the measurement tank 114c.
  • the liquid channel 112 is opened, and the sample is introduced into the measuring tank 114c by gravity.
  • the plug 150 is connected to the liquid channel from the recess 152 in the closing part T11 provided between the filtration tank 114b and the measuring tank 114c. 112, the liquid flow path 112 in this portion is closed.
  • the opening portion S13 provided downstream of the measuring tank 114c is operated, and the sample measured in the measuring tank 114c. Is introduced into the first mixing tank 114f.
  • the opening portion S14 between the first reagent tank 114e and the first mixing tank 114f is similarly opened, and the first reagent is placed in the first mixing tank 114f.
  • the sample is introduced into the mixing tank 114f, and the sample and the first reagent are mixed in the first mixing tank 114f to prepare an intermediate preparation solution.
  • the intermediate preparation liquid prepared in the first mixing tank 114f is introduced into the second mixing tank 114h in the same open state. .
  • the opening reagent S16 between the second reagent tank 114g and the second mixing tank 114h is similarly opened, and the second reagent is introduced into the second mixing tank 114h. Then, the intermediate preparation solution and the second reagent are mixed in the second mixing tank 114h to prepare a measurement solution. Subsequently, the opening portion S17 between the second mixing tank 114h and the measurement tank 114i is similarly opened, and the measurement liquid prepared in the second mixing tank 114h is introduced into the measurement tank 114i. Then, after introducing the measurement liquid into the measurement tank 114i, the liquid channel device 110A is supplied to the detection analysis unit to detect and measure the target component.
  • the process of preparing the measurement liquid in this way it is possible to facilitate the flow of the liquid or to accurately determine the flow rate by appropriately opening and closing communication holes (not shown) provided in each liquid tank as necessary.
  • the flow of the liquid may be controlled, for example, by improving the flow rate.
  • the opening portions S11 to S17, the closing portion T11 that changes the open state to the closed state, the opening portion S15, Since S14 and S16 are included, the same effect as that of the liquid channel device 110 can be obtained.
  • the liquid channel device 110A in this example that is, the liquid channel device 110A in which the lid plate 113A has a two-layer structure and the recesses 151 and 152 are formed in the lid plate 113A can be manufactured by the following method. That is, the liquid flow path 112 and the liquid tank are formed on the substrate 111A, the recesses 151 and 152 are formed on the cover plate 113A, and the plug 115 constituting the opening portions S11 to S17 is connected to the liquid flow path 112.
  • the surface 112a can be manufactured by a method including a third step of laminating the surface (inner surface) on the side where the recesses 151 and 152 are formed in the cover plate 113A.
  • FIG. 27 schematically showing the manufacturing process of the liquid channel device 110A.
  • a wound material 111a ′ is prepared.
  • the sheet 111c ′ is continuously supplied from the wound material 120 of the sheet 111c ′ constituting the inner layer 111c, and a portion corresponding to the liquid flow path 112 is punched out linearly by a punching machine 123a.
  • each liquid tank such as 114c
  • the sheet 111b ′ is continuously supplied from the wound material 121 of the sheet 111b ′ constituting the intermediate layer 111b, and a portion corresponding to the lower part of each liquid tank such as the measuring tank 114c is opened by the punching machine 123b. Punch into a shape.
  • the sheet 111a ′ is continuously supplied from the wound sheet 122 of the sheet 111a ′ constituting the outer layer 111a, and the sheets 111a ′, 111b ′, and 111c ′ are sequentially stacked to manufacture the substrate 111A.
  • each of the sheets 111a ′, 111b ′, and 111c ′ is preferably bonded by an adhesive supplied from an unillustrated adhesive supply device, but depending on the material of each of the sheets 111a ′, 111b ′, and 111c ′. May be bonded together by heat fusion or the like.
  • a wound product 125 of the sheet 113b ′ constituting the inner layer 113b of the cover plate 113A and a wound product 126 of the sheet 113a ′ constituting the outer layer 113a of the cover plate 113A are also prepared.
  • the sheet 113b ′ is continuously supplied from the wound material 125 of the sheet 113b ′ constituting the inner layer 113b, and the portions corresponding to the recesses 151 and 152 are punched by the punching machine 123c.
  • the sheet 113a ′ is continuously supplied from the wound material 126 of the sheet 113a ′ constituting the outer layer 113a of the cover plate 113A, and the sheets 113a ′ and 113b ′ are laminated to manufacture the cover plate 113A.
  • the sheets 113a ′ and 113b ′ are preferably bonded by an adhesive supplied from an adhesive supply device (not shown).
  • an adhesive supply device not shown.
  • heat fusion or the like may be performed. May be pasted together.
  • the sheets 111a ′, 111b ′, 111c ′, 113a ′, and 113b ′ are supplied from the rolls 120, 121, 122, 125, and 126, and the sheets 111b ′, 111c ′, and 113b ′ are supplied.
  • Each of the sheets 111a ′, 111b ′, and 111c ′ is sequentially laminated and bonded to each other.
  • Such a method includes a method of forming a liquid tank, a liquid flow path, and a concave portion, for example, by photolithography, cutting, etc., for each substrate made of a single flat plate and each cover plate, a liquid tank, a liquid flow path, Compared to a method of forming a substrate or a cover plate with a concave portion by injection molding or the like, the manufacturing cost is low, simple and mass production is possible, which is industrially suitable.
  • the method of punching the sheets 111b ′, 111c ′, 113b ′ into a predetermined shape and forming the liquid channel 112, the liquid tank, the recesses 151, 152, etc. is low in cost and excellent in productivity.
  • the sheet 111b ′, 111c ′, 113b ′ is opened in a predetermined shape by a method (laser processing, cutting using a knife, thermal processing, etc.), and the liquid flow path 112, liquid tank, recesses 151, 152 are opened. Etc. may be formed.
  • the substrate 111A of the liquid flow path device 110A is composed of three layers of the outer layer 111a, the intermediate layer 111b, and the inner layer 111c.
  • the substrate 111A is composed of two layers of the outer layer 111a and the inner layer 111c. May be.
  • the liquid flow path 112 and the liquid tank are formed on the sheet 111c ′ forming the inner layer 111c.
  • the formed liquid flow path 112 and the depth of the liquid tank are the same.
  • styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin are preferable because they are transparent and the state of the liquid flowing through the liquid flow path 12 can be visually observed.
  • a wound material of resin is used as the material of the substrate 111A and the cover plate 113A, and a preferable manufacturing method of the liquid flow path device 110A at that time is described. Therefore, as the sheet material, Thus, resin is illustrated.
  • the manufacturing method is not particularly limited.
  • a transparent material other than resin such as glass is used for the substrate and the cover plate. It is also possible to form a liquid flow path, a liquid tank, a recess, etc. by applying a method such as cutting to the above.
  • each of the sheets 111a ′, 111b ′, and 111c ′ can be set as appropriate.
  • the thickness of the sheet 111c ′ that forms the inner layer 111c is the liquid to be formed.
  • the sum of the thickness of the sheet 111c ′ forming the inner layer 111c and the sheet 111b ′ forming the intermediate layer 111b corresponds to the depth of the flow path 112 and corresponds to the total depth of the liquid tank. Therefore, the thickness of the sheet 111b ′ and the sheet 111c ′ is determined in consideration of the depth required for the liquid tank, the liquid flow path 12, and the like.
  • the thickness of the sheet 111b ′ is preferably 25 to 500 ⁇ m, and the thickness of the sheet 111c ′ is preferably 10 to 300 ⁇ m. Further, the thickness of the sheet 111a ′ is preferably 50 ⁇ m or more, more preferably 100 to 1000 ⁇ m, when the opening portions S11 to S17 and the closing portion T11 are operated by pressing the lid plate 113A. With such a thickness, it sufficiently acts as a support layer for the liquid channel device 110A. On the other hand, when the substrate 111A is pressed, the substrate 111A needs to have a restoring force that bends when a load is applied by the pressing operation and then returns to the original when the load is removed. .
  • the thickness of the sheet 111a ′ is preferably 10 to 300 ⁇ m.
  • the width of the liquid flow path 112 is preferably 25 to 2000 ⁇ m, more preferably 500 to 2000 ⁇ m, and the volume of the liquid tank is preferably 50 to 50000 ⁇ l, more preferably 100 to 1000 ⁇ l.
  • the waste liquid tank 114d and the like do not have a particularly suitable volume, and can be freely designed according to the function of each liquid tank.
  • each sheet 113a ′, 113b ′ can also be set as appropriate.
  • the thickness of the sheet 113b ′ forming the inner layer 113b is set to the recessed portion 151 formed. This corresponds to a depth of 152. Therefore, the thickness of the sheet 113b ′ is determined in consideration of the depth required for the recesses 151 and 152. Further, as shown in FIGS.
  • the seat 113a ′, 113b ′ needs to have a restoring force that bends when a load is applied by a pressing operation and then returns to the original when the load is removed. Considering this point, it is necessary to determine these thicknesses.
  • the thickness of the sheet 113a ′ is preferably 10 to 300 ⁇ m
  • the thickness of the sheet 113b ′ is preferably 25 to 500 ⁇ m.
  • the thickness of the sheet 113a ′ is preferably 50 ⁇ m or more.
  • the weak adhesive layers 115a and 117a and the strong adhesive layers 116a and 118a for holding the stoppers 115 and 150 are bonded to the predetermined steps.
  • An unillustrated adhesive layer forming step to be formed at the position is performed.
  • a method of selecting an appropriate adhesive and applying it to a predetermined position is suitable.
  • the strong adhesive layers 116a and 118a and the weak adhesive layers 115a and 117a can be appropriately selected from conventionally known adhesives according to the material of the substrate 111A, the cover plate 113A, the plugs 115 and 150, etc.
  • the adhesive force (adhesive strength) of the adhesive forming the strong adhesive layers 116a and 118a needs to be stronger than the adhesive force of the adhesive forming the weak adhesive layers 115a and 117a. If the adhesive force of the adhesive forming the strong adhesive layers 116a and 118a is less than or equal to the adhesive force of the adhesive forming the weak adhesive layers 115a and 117a, even if a pressing operation is performed at the opening portions S11 to S17 and the closing portion T11.
  • the plugs 115 and 150 cannot be moved from the liquid channel 112 into the recess 151, moved from the recess 152 to the liquid channel 112, or cannot be held there after the movement. In that case, the liquid channel 112 cannot be opened or closed.
  • the adhesive strength of the adhesive forming the strong adhesive layers 116a and 118a is 0.1 N / cm or more larger than the adhesive strength of the adhesive forming the weak adhesive layers 115a and 117a. Further, it is preferably large in the range of 0.1 to 30 N / cm.
  • the adhesive force of the adhesive forming the strong adhesive layers 116a and 118a is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layers 115a and 117a, the opening portions S11 to S17 and the closing portion T11 are formed. It can be operated reliably. On the other hand, it is difficult to configure these adhesive layers so that the difference in adhesive strength exceeds 30 N / cm.
  • the adhesive strength of the strong adhesive layers 116a and 118a is preferably in the range of 1 to 30 N / cm
  • the adhesive strength of the weak adhesive layers 115a and 117a is preferably in the range of 0.05 to 5 N / cm.
  • Examples of the adhesive used for the strong adhesive layers 116a and 118a and the weak adhesive layers 115a and 117a include acrylic, rubber, polyurethane, polyester, and silicon.
  • the strong adhesive layers 116a and 118a may be made of acrylic or rubber, and may further include a nonwoven fabric or polyester fiber as a core material.
  • the weak adhesive layers 115a and 117a it is preferable to use acrylic or silicon-based ones.
  • the glass transition temperature of the resin constituting each adhesive is appropriately adjusted, or the adhesive
  • additives such as a tackifier, a hardening
  • adheresive strength here means 180 degree peeling adhesive strength against the stainless steel plate of JIS Z 0237.
  • a plug body 115 is formed at a part of the liquid flow path 112 formed on the substrate 111A in the first step, that is, at each position where the opening portions S11 to S17 are provided.
  • the plug 150 is formed in the recess 152 corresponding to the closing portion T11 among the recesses 151 and 152 formed in the cover plate 113A.
  • the plug body 115 is formed by applying a plug body forming material for forming the plug body 115 to a predetermined position of the substrate 111A that is continuously supplied, such as a printing machine, a dispenser, and a coater (roll coater, knife coater, etc.). This is performed by the coating method 124a.
  • the plug body 150 is also formed by applying and filling the plug body forming material into the recess 152 using the same coating device 124b.
  • a resin composition having a viscosity in the range of 30 to 600 dPa ⁇ s is preferably used, and the resin composition in the above viscosity range is more preferable if it does not contain a solvent.
  • the resin component contained in the resin composition has good coating properties (printability, dispensing properties, etc.), sealing ability as a plug and stability thereof, and viscosity in the above range when used as a plug forming material.
  • the resin composition may contain an appropriate plastic component.
  • the resin composition may contain a filler for adjusting the viscosity of the plug-forming material, and examples thereof include precipitated barium sulfate, talc, acicular silicon oxide, and hollow beads. Can be used. A solvent is mix
  • the plugs 115 and 150 can be continuously and efficiently formed at predetermined positions.
  • each step such as a heat drying process and a curing process is performed as necessary depending on the composition of the plug body forming material.
  • the surface on the side where the recesses 151 and 152 of the cover plate 113A are formed is laminated and bonded to the flow path forming surface 112a of the substrate 111A.
  • the substrate 111A and the cover plate 113A are preferably bonded by an adhesive supplied from an adhesive supply device (not shown).
  • the substrates 111A and the cover plate 113A are bonded together by heat fusion or the like. May be.
  • the continuum of the liquid channel device 110A thus manufactured may be wound up as shown in FIG. 27 to be in the state of a wound object 127 or in a folded state. Moreover, you may be set as the single wafer type cut
  • a process of forming a line such as a perforation or a concave line between the liquid channel devices 110A may be performed. Thereby, it becomes easy to bend between each liquid flow-path apparatus 110A, and can make it easy to fold the continuous body of 110 A of liquid flow-path apparatuses. Moreover, it can be made easy to separate into a single wafer type.
  • the plugs 115 and 150 are held in place by the weak adhesive layers 115a and 117a in advance, and then the liquid is pressed by a pressing operation.
  • the liquid channel 112 is moved from the flow path 112 to the recess 151 or from the recess 152 to the liquid flow path 112 and is held at a predetermined position by the strong adhesion layers 116a and 118a.
  • the present invention is not limited to a mode in which the stoppers 115 and 150 are moved to a predetermined position and held there by a method using the difference in adhesive force.
  • the plugs 115 and 150 are recessed in the open state. As long as it is held tightly by elastic force or the like in 151 and 152, the weak adhesive layer 117a and the strong adhesive layer 116a are not necessarily provided in the recesses 151 and 152. Further, in the closed state, the bottoms 112b of the liquid flow path 112 are, for example, shown in FIG. 28 so that the plugs 115 and 150 are securely held at predetermined positions of the liquid flow path 112 and do not move from there.
  • a pair of plug receivers 119a and 119b that sandwich the plugs 115 and 150 may be formed. If the plug body receivers 119a and 119b are formed of, for example, an elastic body, the plug bodies 115 and 150 can be held so as not to be displaced from a predetermined position of the liquid channel 112 by an elastic force. Further, for example, as illustrated in FIG.
  • the planar view shape of the plug body 115 is set to a diamond shape, and the maximum width W1 is set larger than the width W2 of the liquid flow path 112, and In the closed state, both ends of the plugs 115 and 150 in the width direction are formed on both side walls of the liquid channel 112 by, for example, a method of forming locking recesses that lock both ends of the plug 115 in the width direction.
  • the portion may be locked in the locking recess so as not to be displaced from a predetermined position of the liquid channel 112 along the liquid channel (vertical direction in the figure).
  • the plug body 115 having a diamond shape in plan view is illustrated, but the maximum width W1 of the plug body 115 is formed larger than the width W2 of the liquid channel 112, and plugs are formed on both side walls of the liquid channel.
  • the planar view shape of the plug is not limited to a rhombus.
  • the plug body 115 includes a weak adhesive layer 115a, 117a, and a strong adhesive layer 116a, 118a, a plug body receiver 119a, 119b, and a planar view of the plug bodies 115, 150 having a rhombus shape. , 150 may be used in combination as a means for holding.
  • the cover plate 113A is composed of two layers of the outer layer 113a and the inner layer 113b, and the substrate 111A is composed of three layers of the outer layer 111a, the intermediate layer 111b, and the inner layer 111c.
  • the recessed parts 151 and 152 illustrated the form formed in the cover plate 113A.
  • the cover plate 113 is composed of one layer, while the substrate 111B has four layers of an outer layer 141a, an outer intermediate layer 141b, an inner intermediate layer 141c, and an inner layer 141d.
  • a configuration in which the concave portions 151 and 152 are formed on the bottom portion 112 b of the liquid channel 112 instead of the lid plate 113 is illustrated.
  • the liquid flow path device 110B in this example includes an outer layer 141a, an outer intermediate layer 141b stacked on the inner side, an inner intermediate layer 141c stacked on the inner side, and an inner side thereof.
  • the inner layer 141d is laminated in four layers.
  • an upper portion (portion on the lid plate 113 side of the liquid tank) of the liquid tank (only the sample charging tank 114a and the filtration tank 114b are shown in FIG. 30) and the liquid channel 112 are formed.
  • the inner intermediate layer 141c an intermediate part of the liquid tank (an intermediate part excluding the part on the bottom side of the liquid tank among the parts other than the upper part) and concave parts 151 and 152 are formed.
  • the inner intermediate layer 141 c has a surface on the inner layer 111 d side that forms the bottom 112 b of the liquid channel 112.
  • the outer intermediate layer 141b is formed with a lower part of the liquid tank (a part on the bottom side of the liquid tank, and a part other than the upper part and the intermediate part).
  • the surface on the inner intermediate layer 141c side forms the bottom of the recesses 151 and 152.
  • the outer layer 141a is disposed on the outermost side of the substrate 111B, and the surface on the outer intermediate layer 141b side forms the bottom of the liquid tank.
  • the lid plate 113 is composed of only one layer.
  • the opening portions S11 to S17 are configured so as to block a part of the liquid channel 112 in the liquid channel 112, as described with reference to S11 and S12 in FIG.
  • a resin plug 115 is disposed to seal the flow of the liquid and to close this portion.
  • a concave portion 151 that can accommodate the plug body 115 is formed at a position facing the plug body 115 at the bottom 112 b of the liquid flow path 112.
  • the plug body 115 in this example is fixed to the inner surface of the lid plate 113 by a weak adhesive layer 115a in a closed state (top portion) in contact with the lid plate 113. .
  • the height of the plug 115 is formed slightly higher than the height of the liquid flow path, and the bottom 115c side is arranged in a state of being slightly fitted in the recess 151 in a liquid-tight manner. Then, the stopper 115 moves from the liquid channel 112 into the recess 151 by an operation of pressing the lid plate 113 or the bottom 112b of the liquid channel 112 where the opening portions S11 and S12 are provided from the outside. Thus, the liquid channel 112 is changed from the closed state to the open state.
  • FIG. 32A and FIG. 32B by taking the opening portion S1 as an example, when a load is applied by pressing the lid plate 113 from the outside as indicated by an arrow C, the lid as shown in FIG. 32A.
  • the plate 113 bends, and the strong adhesive layer 116a of the recess 151 comes into contact with the bottom 115c of the stopper 115, and these stick together.
  • the cover plate 113 is restored to its original state by its restoring force.
  • the plug 115 is placed in the recess 151 by the action of the strong adhesive layer 116a. It is held in a state of being housed in the container.
  • the plug 115 is separated from the cover plate 113 so that the liquid can flow therethrough.
  • the lid 115 is pressed by pressing the lid plate 113 of the portion where the opening portions S11 to S17 are provided from the outside, and then the plug 115 is removed by a pressing operation for removing the load.
  • the liquid channel 112 moves from the liquid channel 112 into the recess 151, and as a result, the liquid channel 112 in this portion changes from the closed state to the open state.
  • the load is applied by pressing the cover plate 113 from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the opening portion S11 is provided is from the outside, that is, the substrate 111B.
  • the plug body 115 may be moved from the liquid flow path 112 into the recess 151 by an operation of pressing from the outside.
  • the closing portion T11 of the liquid channel device 110B includes a resin plug 150, which is a recess formed in the bottom 112b of the liquid channel 112. It is housed in 152.
  • the inner intermediate layer 141c of the substrate 111B is opened by punching or the like to form the recess 152, and the plug 150 is disposed therein.
  • the plug 150 of this example is housed in the recess 152 with its bottom adhered to the recess 152 by the weak adhesive layer 117a.
  • the height of the plug 150 is slightly higher than the height of the liquid channel 112.
  • the bottom side of the plug 150 is slightly inserted into the recess 152. It is supposed to be in the state.
  • a strong adhesion layer 118 a is formed on the inner surface of the lid plate 113 at a position facing the top of the plug 150. Then, the stopper 150 moves from the inside of the recess 152 to the liquid flow path 112 by an operation of pressing the lid plate 113 or the bottom 112b of the liquid flow path 112 in the portion where the closing portion T11 is provided, The liquid channel 112 is changed from the open state to the closed state.
  • FIGS. 33A and 33B when a load is applied by pressing the cover plate 13 from the outside as indicated by an arrow D, the cover plate 113 is bent as shown in FIG. The strong adhesion layer 118a and the top of the plug 150 are in contact with each other, and these adhere to each other. Then, when the load is removed thereafter, as shown in FIG. 33B, the cover plate 113 is restored to its original state by its restoring force. At this time, the plug 150 is covered by the strong adhesive layer 118a. It is held in an adhesive state inside. As a result, the plug 150 moves from the recess 152 to the liquid channel 112. Thus, the liquid flow path 112 is blocked by the plug 150, and the liquid cannot flow through this portion.
  • the stopper 150 is pushed from the outside by pressing the cover plate 113 of the portion where the closing portion T11 is provided, and then the plug 150 is removed from the recess 152 by a pressing operation to remove the load.
  • the liquid flow path 112 moves to the liquid flow path 112, and as a result, the liquid flow path 112 in this portion changes from the open state to the closed state.
  • the load is applied by pressing the cover plate 113 from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the closing portion T11 is provided is from the outside, that is, the substrate 111B.
  • the stopper 150 may be moved to the liquid flow path 112 by an operation of pressing from the outside of the liquid.
  • the liquid flow path device 110B includes a first step of forming the liquid flow path 112, the liquid tank, and the recesses 151 and 152 in the substrate 111B, and the plug body 115 constituting the opening portions S11 to S17.
  • the wound material 128 of the sheet 141d ′ constituting the inner layer 141d of the substrate 111B, the wound material 129 of the sheet 141c ′ constituting the inner intermediate layer 141c, and the outer side A wound product 130 of the sheet 141b ′ constituting the intermediate layer 141b and a wound product 131 of the sheet 141a ′ constituting the outer layer 141a are prepared.
  • the sheet 141d ′ is continuously supplied from the wound material 128 of the sheet 141d ′ constituting the inner layer 141d, and a portion corresponding to the liquid flow path 112 is punched out linearly by a punching machine 123a.
  • a portion corresponding to the upper portion of each liquid tank such as 114c is punched into a hole.
  • the sheet 141c ′ is continuously supplied from the wound material 129 of the sheet 141c ′ constituting the inner intermediate layer 141c, and the portion corresponding to the intermediate portion of each liquid tank such as the measuring tank 114c by the punching machine 123b. Are punched into holes, and the portions corresponding to the recesses 151 and 152 are also punched.
  • the sheet 141b ′ is continuously supplied from the wound material 130 of the sheet 141b ′ constituting the outer intermediate layer 141b, and a portion corresponding to the lower part of each liquid tank such as the measuring tank 114c is formed by the punching machine 123c. Punched into a hole.
  • each sheet 141a ′, 141b ′, 141c ′, 141d ′ is preferably bonded by an adhesive supplied from an adhesive supply device (not shown), but each sheet 141a ′, 141b ′, 141c ′. , 141d ′ may be bonded together by heat fusion or the like. Furthermore, you may use the sheet
  • the weak adhesive layers 115a and 117a and the strong adhesive layers 116a and 118a for holding the stoppers 115 and 150 are placed at predetermined positions.
  • An unillustrated adhesive layer forming step to be formed is performed.
  • the sheet 113 ′ is continuously supplied from the wound material 132 of the sheet 113 ′ constituting the cover plate 113 so as to correspond to the positions of the opening portions S11 to S17.
  • a plug 115 is formed on the inner surface of 113.
  • the plug 150 is formed in the recess 152 constituting the closing portion T11 among the recesses 151 and 152 formed in the bottom 112b of the liquid channel 112 in the first step.
  • the plug body 115 is formed by applying a plug body forming material for forming the plug body 115 with a coating device 124a such as a printing machine, a dispenser, or a coater (roll coater, knife coater, etc.).
  • the plug body 150 can be preferably formed by a method in which the plug body forming material is applied and filled in the concave portion 152 formed in the substrate 111B that is continuously supplied by the same coating device 124b.
  • the plug forming material those exemplified in the fifth embodiment can be suitably used as well.
  • the inner surface of the cover plate 113 that is, the surface on which the plug 115 is formed is laminated on the flow path forming surface 112a of the substrate 111B and bonded.
  • the substrate 111B and the cover plate 113 are preferably bonded by an adhesive supplied from an adhesive supply device (not shown).
  • the substrates 111B and the cover plate 113 are bonded together by heat fusion or the like. May be.
  • the continuous body of the liquid flow path device 110B thus manufactured may be wound into a wound product 133 or a folded state. Good. Moreover, you may be set as the single wafer type cut
  • the shape and material of the plugs 115 and 150, the shape of the recesses 151 and 152, the material of the substrate 111B on which the recesses 151 and 152 are formed, and the like If the plugs 115 and 150 are tightly held in the recesses 151 and 152 by an elastic force or the like in the opened state by adjusting, the weak adhesive layer 117a or the strong adhesive layer 117a or the strong adhesive layer is not necessarily in the recesses 151 and 152. The layer 116a may not be provided. Further, as in the case of the fifth embodiment, a pair of plug receivers 119a and 119b that sandwich the plugs 115 and 150 may be formed.
  • both end portions in the width direction of the plugs 115 and 150 are locked by the locking recesses so that the liquid channel 112 is not displaced from a predetermined position along the liquid channel (vertical direction in the figure). May be. Furthermore, these methods may be used in combination.
  • the liquid flow path 112 is formed only on one surface of the substrates 111A and 111B, but the liquid flow path 112 is formed on both surfaces of the substrates 111A and 111B.
  • a channel 112 may be formed.
  • the form etc. which can open and close a hole may be sufficient.
  • an opening part and a closing part having the same configuration as the opening parts S11 to S17 and the closing part T11 provided in the liquid channel 112 may be provided in the communication hole.
  • the inner volume of the liquid tank is reduced by the operation of pressing the lid plate 113 corresponding to the liquid tank or the bottom of the liquid tank from the outside, The liquid is discharged and sent to the downstream side.
  • the liquid tank provided with such a liquid feeding part is preferably provided with a backflow prevention part such as a weir plate for preventing the backflow of the liquid to the upstream side.
  • the liquid channel devices 110, 110A, and 110B are set in the centrifuge so that the sample introduction tank 114a side is located on the rotation center side and the measurement tank 114i side is located on the outer periphery side of the rotation. 110A and 110B may be rotated so that centrifugal force acts from the upstream side to the downstream side of the liquid channel 112, and as a result, the liquid may flow.
  • a pressure contact disk can also be used. The pressure contact disk presses a predetermined position while moving in the radial direction of the rotation from the rotation center side to the rotation outer peripheral side on the surface of the cover plate 113, 113A of the liquid flow path device 110, 110A, 110B. is there.
  • the liquid flow path 112 In addition to moving the liquid using gravity or centrifugal force, the liquid flow path 112, a part of the liquid tank, or both of them are heated to expand the air in the liquid flow path 112 or the liquid tank.
  • an oxygen absorbent such as iron powder that easily oxidizes
  • a method of injecting the sample into the sample introduction tank 114a a method of piercing the needles of the syringe into the cover plates 113 and 113A is exemplified.
  • the sample may be injected from there.
  • the sample injection hole may be covered with a protective tape and injected by piercing the protective tape into the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.
  • sample and reagent through which the liquid channel devices 110, 110A, and 110B are circulated there are no particular limitations on the sample and reagent through which the liquid channel devices 110, 110A, and 110B are circulated, and a sample and a reagent that have been conventionally used in the medical field, the environmental field, and the like can be used in appropriate combination.
  • samples of blood whole blood
  • plasma serum
  • buffy coat urine
  • saliva saliva
  • sputum etc.
  • viruses viruses
  • bacteria, mold, yeast, animal and plant cells, etc. can be mentioned.
  • DNA or RNA isolated from these may be used, and a sample obtained by subjecting them to any pretreatment or dilution may be used.
  • the liquid channel devices 110, 110A, and 110B exemplified above include a filtration tank 114b for performing a filtration process on the sample flowing from the sample input tank 114a downstream of the sample input tank 114a. . Therefore, when such liquid flow path devices 110, 110A, and 110B are used, for example, in the case where blood cells are removed from blood by filtration, a sample that conventionally had to be previously filtered by another filtration device is previously stored. Without filtration, the sample can be used as it is in the sample introduction tank 14a of the liquid flow path devices 110, 110A, 110B.
  • the reagent is not particularly limited and may be appropriately selected depending on the target component.
  • a reagent containing an antibody against the antigen is preferable.
  • a reagent containing, for example, an antibody is sealed in the first reagent tank 114e or the second reagent tank 114g in advance, and these reagents and a sample containing, for example, an antigen are mixed in the first mixing tank.
  • An example in which the antigen is captured by the antibody by mixing in 114f or the second mixing tank 114h is illustrated.
  • the form in which the antigen is captured by the antibody is not limited to such a form.
  • the antibody or the magnetic beads carrying the antibody are placed in the liquid tanks of the liquid channel devices 110, 110A, 110B or in the middle of the liquid channel.
  • the antigen in the sample may be captured by the antibody by allowing the sample to flow therethrough.
  • an appropriate reagent is introduced from the sample introduction tank 114a or the like with a syringe or the like, and the above-described liquid feeding unit is appropriately used as necessary to wash or denature the captured antigen. Or can be increased (concentration increase) or separated to increase the analysis accuracy.
  • the reactions performed in the liquid channel devices 110, 110A, and 110B are not limited to antigen-antibody reactions, but include various chemical reactions, PCR (polymerase chain reaction) that amplifies DNA, and reactions that capture proteins such as DNA. it can. A plurality of reactions may be combined, or some reaction may not necessarily be performed, for example, only the mixing process is performed in the liquid channel device 110. Thus, there is no limitation on the method of using the liquid channel devices 110, 110A, 110B.
  • various treatments can be performed on the liquid flow path 112 and the liquid tank.
  • various chemical treatments using acids, alkalis, etc. physical treatments using latex, fluorescent substances, etc., biochemical treatments using antigens, antibodies, DNA, etc.
  • Surface treatment effects such as treatment and water repellent treatment can be obtained.
  • coating treatment, plasma treatment, flame treatment, and the like may be performed.
  • the liquid flow path 112 may be provided with a baffle plate, a stirring plate, a protrusion, or formed with a water diverting shape, if necessary, so that the flowing liquid is in a uniform mixed state.
  • the inside of the liquid channel 112 and the liquid tank may be pressurized as necessary (pressure treatment) or depressurized (vacuum treatment).
  • the liquid channel device 110, 110A, 110B of the liquid channel device 110, 110A, 110B such as the lid plate 113, 113A, the liquid channel 112, the liquid tank, the substrate 111, 111A, 111B, etc. , 110A, 110B, the contents and procedure of the operation performed, the name of the liquid tank (for example, “weighing tank”, etc.), etc. may be directly printed. Or you may stick the display sticker etc. which printed the content and procedure of operation, the name of a liquid tank, etc., or may provide the marking used as a certain mark. Further, for example, only a part of the cover plates 113 and 113A may be made transparent so that the portions become conspicuous.
  • liquid flow path devices 110, 110A, and 110B Conventionally known optical means, electrical means, and the like can be appropriately employed as the detection / analysis unit for the measurement liquid prepared by the liquid flow path devices 110, 110A, and 110B. At that time, the liquid flow path devices 110, 110A, and 110B may be heated or cooled as necessary.

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Abstract

A liquid flow path device configured in such a manner that a liquid flow path can be easily set from a closed state to an open state.  A liquid flow path device, wherein a liquid flow path (12) in which liquid consisting of a sample and/or a reagent flows and a measuring vessel (14c) in which the liquid is contained are formed in at least one surface of a substrate (11A), and wherein a lid plate (13) is layered on the substrate (11A).  The measuring vessel (14c) is provided with a liquid sending section (P1) for sending the liquid in the vessel to the downstream side.  The liquid sending section (P1) is operated by pressing, from the outside, that portion of the lid plate (13) which corresponds to the measuring vessel (14c).

Description

液体流路装置とその製造方法Liquid channel device and manufacturing method thereof

　本発明は、例えば血液中の抗原の検出、分析などに好適に使用される平板状の液体流路装置とその製造方法に関する。
　本願は、２００８年１０月２８日に日本に出願された特願２００８－２７６４６８号、２００９年０８月２５日に日本に出願された特願２００９－１９４５９０号、２００９年０８月２５日に日本に出願された特願２００９－１９４５９１号、並びに２００９年０８月２５日に日本に出願された特願２００９－１９４５９２号に基づき優先権を主張し、その内容をここに援用する。 The present invention relates to a plate-like liquid flow path device suitably used for detecting and analyzing antigens in blood, for example, and a method for producing the same.
This application includes Japanese Patent Application No. 2008-276468 filed in Japan on October 28, 2008, Japanese Patent Application No. 2009-194590 filed in Japan on August 25, 2009, and Japanese Application on August 25, 2009. Claimed priority based on Japanese Patent Application No. 2009-194591 filed and Japanese Patent Application No. 2009-194592 filed in Japan on Aug. 25, 2009, the contents of which are incorporated herein by reference.

　近年、医療分野、環境分野などでは、液体試料中の微量成分の検出、分析が頻繁に行われており、その際、例えば医療分野では、基板に流路が形成されたマイクロチップと呼ばれる液体流路装置が使用される場合が多い。
　例えば特許文献１には、マイクロチップに形成された液体流路内で、抗体を含有する試薬と血液とを混合、反応させた後、該マイクロチップごと検出装置に供して、抗原抗体反応を検出する技術が記載されている。また、例えば特許文献２には、回転可能なディスクの半径方向に流路を複数形成し、この流路の一部にあらかじめ抗体を固定しておき、その後、流路に体液を流通させることによって、抗原抗体反応により体液中の抗原を抗体に捕捉させるディスク状の液体流路装置が開示されている。 In recent years, detection and analysis of trace components in a liquid sample are frequently performed in the medical field, the environmental field, and the like. In this case, for example, in the medical field, a liquid flow called a microchip having a channel formed on a substrate is used. Road equipment is often used.
For example, in Patent Document 1, an antibody-containing reagent and blood are mixed and reacted in a liquid flow path formed on a microchip, and then the whole microchip is supplied to a detection device to detect an antigen-antibody reaction. The technology to do is described. Further, for example, in Patent Document 2, a plurality of flow paths are formed in the radial direction of a rotatable disc, an antibody is fixed in advance in a part of the flow paths, and then body fluid is circulated through the flow paths. In addition, a disk-shaped liquid flow path device is disclosed in which an antigen in a body fluid is captured by an antibody by an antigen-antibody reaction.

特開２００７－１３９５００号公報JP 2007-139500 A 特開平０５－００５７４１号公報JP 05-005741 A

　しかしながら、試薬や血液を液体流路中に流通させるために、特許文献１の液体流路装置では別途マイクロポンプを使用する必要があり、特許文献２の液体装置ではディスクを回転させる装置が必要であった。
また、このような従来の液体流路装置では、液体流路を閉止したり開通させたりすることができず、目的の成分の検出、分析に不都合が生じることがあった。
　また、このような従来の液体流路装置では、液体流路の一部を閉止状態から開通状態、開通状態から閉止状態、または閉止したり開通させたりすることができず、目的の成分の検出、分析に不都合が生じることがあった。
　本発明の目的は、液体を流通させるための装置を別途必要とすることなく、簡便に液体流路を閉止状態から開通状態にでき、即ち液体を簡便かつ円滑に流路に流通させることのできる液体流路装置を低コストで提供することである。
　更に、簡便に液体流路を閉止状態から開通状態、開通状態から閉止状態に、または開通状態から閉止状態にしたり、閉止状態から開通状態にしたりできる液体流路装置を低コストで提供することである。 However, in order to circulate the reagent and blood in the liquid flow path, the liquid flow path device of Patent Document 1 needs to use a separate micropump, and the liquid device of Patent Document 2 requires a device for rotating the disk. there were.
Further, in such a conventional liquid channel device, the liquid channel cannot be closed or opened, which may cause inconvenience in detection and analysis of the target component.
In addition, in such a conventional liquid flow path device, a part of the liquid flow path cannot be closed from the closed state to the open state, from the open state to the closed state, or to be closed or opened. Inconvenient analysis may occur.
The object of the present invention is to make it possible to easily change the liquid flow path from the closed state to the open state without requiring a separate device for flowing the liquid, that is, to allow the liquid to flow through the flow path easily and smoothly. It is to provide a liquid channel device at a low cost.
Furthermore, by providing a liquid flow path device that can easily change the liquid flow path from the closed state to the open state, from the open state to the closed state, from the open state to the closed state, or from the closed state to the open state, at low cost. is there.

　本発明の液体流路装置は、基板の少なくとも片面に、試料および試薬の少なくとも一方からなる液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、前記液槽の少なくとも１つは、該液槽内の液体を液槽外に送液する送液部を有し、該送液部は、前記液槽に対応する部分の蓋板または前記液槽の底部を外側から押圧する操作により作動することを特徴とする。
　本発明の液体流路装置は、前記液体流路の一部を閉止状態から開通状態にする開通部と、開通状態から閉止状態にする閉止部とをさらに有し、前記蓋板は、該蓋板の表面を構成する第１基材層と、該第１基材層の内側に形成された強粘着層と、該強粘着層の内側に形成された第２基材層と、該第２基材層の内側に形成され、前記流路形成面に粘着する弱粘着層とを有し、前記開通部では、前記液体流路に第１凸部が形成され、該第１凸部の頂部と前記弱粘着層とが粘着し、かつ、前記強粘着層と前記第２基材層とが離間し、前記閉止部では、前記液体流路に第２凸部が形成され、該第２凸部の頂部と前記弱粘着層とが離間し、かつ、前記強粘着層と前記第２基材層との間にはスペーサ部材が介在し、該スペーサ部材と前記強粘着層とが粘着し、前記送液部では、前記強粘着層と前記第２基材層との間にはスペーサ部材が介在し、該スペーサ部材と前記強粘着層とが粘着していることが好ましい。
　前記基板は、外層と、該外層の内側に積層した中間層と、該中間層の内側に積層した内層とからなり、前記内層には、前記液槽の上部と、前記液体流路と、前記第１凸部と、前記第２凸部とが形成され、前記中間層には、前記液槽の下部が形成されていることが好ましい。
　または、前記基板は、外層と、該外層の内側に積層した内層とからなり、前記内層には、前記液槽と、前記液体流路と、前記第１凸部と、前記第２凸部とが形成されていることが好ましい。
　前記送液部が設けられた前記液槽には、該送液部で送液される液体の逆流を防止する逆流防止部が設けられていることが好ましい。
　また、その場合、逆流防止部は、前記内層に形成されていることが好ましい。
前記送液部は、前記液槽の底部を外側から押圧する操作により作動するものである場合、前記底部は、外方に膨出して形成されていることが好ましい。 In the liquid channel device of the present invention, a liquid channel in which a liquid consisting of at least one of a sample and a reagent flows and at least one liquid tank in which the liquid is accumulated are formed on at least one surface of the substrate, A liquid flow path device in which a cover plate is laminated on a flow path forming surface on which the liquid flow path and the liquid tank are formed, and at least one of the liquid tanks is configured to transfer liquid in the liquid tank. It has a liquid feeding part which feeds liquid outside, and this liquid feeding part is actuated by operation which presses the lid of the part corresponding to the liquid tank, or the bottom of the liquid tank from the outside.
The liquid flow path device of the present invention further includes an opening part that changes a part of the liquid flow path from a closed state to an open state, and a closing part that changes the open state from the open state to the closed state. A first base layer constituting the surface of the plate, a strong adhesive layer formed inside the first base layer, a second base layer formed inside the strong adhesive layer, and the second A weak adhesive layer that is formed inside the base material layer and adheres to the flow path forming surface, and in the opening portion, a first convex portion is formed in the liquid flow channel, and a top portion of the first convex portion And the weak adhesive layer adhere to each other, and the strong adhesive layer and the second base material layer are separated from each other, and in the closed portion, a second convex portion is formed in the liquid channel, and the second convex The top of the part and the weak adhesive layer are separated from each other, and a spacer member is interposed between the strong adhesive layer and the second base material layer, and the spacer member and the strong adhesive layer adhere to each other. In the feeding section, the spacer member is interposed between the strong adhesive layer and the second base layer, it is preferable that the said spacer member and said strong adhesive layer is adhered.
The substrate includes an outer layer, an intermediate layer laminated on the inner side of the outer layer, and an inner layer laminated on the inner side of the intermediate layer. The inner layer includes an upper part of the liquid tank, the liquid channel, It is preferable that a 1st convex part and the said 2nd convex part are formed, and the lower part of the said liquid tank is formed in the said intermediate | middle layer.
Alternatively, the substrate includes an outer layer and an inner layer laminated on the inner side of the outer layer, and the inner layer includes the liquid tank, the liquid flow path, the first convex portion, and the second convex portion. Is preferably formed.
It is preferable that the liquid tank provided with the liquid feeding part is provided with a backflow prevention part for preventing the backflow of the liquid fed by the liquid feeding part.
In that case, the backflow prevention part is preferably formed in the inner layer.
When the liquid feeding part is operated by an operation of pressing the bottom part of the liquid tank from the outside, the bottom part is preferably formed to bulge outward.

　更に、本発明の液体流路装置は、基板の少なくとも片面に、試料および試薬の少なくとも一方からなる液体が流通する液体流路が形成され、前記基板の前記液体流路が形成された流路形成面には蓋板が積層した液体流路装置であって、前記液体流路の一部を閉止状態から開通状態にする開通部を有し、前記蓋板は、該蓋板の表面を構成する第１基材層と、該第１基材層の内側に形成された強粘着層と、該強粘着層の内側に形成された第２基材層と、該第２基材層の内側に形成され、前記流路形成面に粘着する弱粘着層とを有し、前記開通部では、前記液体流路に第１凸部が形成され、該第１凸部の頂部と前記弱粘着層とが粘着し、かつ、前記強粘着層と前記第２基材層とが離間していることを特徴とする。
　液体流路装置は、前記液体流路の一部を開通状態から閉止状態にする閉止部をさらに有し、前記閉止部では、前記液体流路に第２凸部が形成され、該第２凸部の頂部と前記弱粘着層とが離間し、かつ、前記強粘着層と前記第２基材層との間にはスペーサ部材が介在し、該スペーサ部材と前記強粘着層とが粘着していることが好ましい。
　前記液体流路には、一定量の液体を計量する計量槽が設けられ、少なくとも該計量槽の上流には前記閉止部が設けられ、下流には前記開通部が設けられていることが好ましい。
　前記計量槽には、前記一定量を超えた液体をオーバーフローさせるオーバーフロー部が設けられていることが好ましい。 Furthermore, in the liquid flow path device of the present invention, a liquid flow path in which a liquid consisting of at least one of a sample and a reagent flows is formed on at least one surface of the substrate, and the flow path formation in which the liquid flow path of the substrate is formed. A liquid channel device in which a cover plate is stacked on a surface, the liquid channel device having an opening portion that opens a part of the liquid channel from a closed state to an open state, and the cover plate constitutes a surface of the cover plate A first base material layer, a strong adhesive layer formed inside the first base material layer, a second base material layer formed inside the strong adhesive layer, and an inside of the second base material layer A weak adhesive layer that is formed and adheres to the flow path forming surface, and in the opening portion, a first convex portion is formed in the liquid channel, and the top of the first convex portion and the weak adhesive layer Is adhered, and the strong adhesion layer and the second base material layer are separated from each other.
The liquid flow path device further includes a closing portion that changes a part of the liquid flow path from an open state to a closed state, and in the closing portion, a second protrusion is formed in the liquid flow path, and the second protrusion The top of the part and the weak adhesive layer are separated from each other, and a spacer member is interposed between the strong adhesive layer and the second base material layer, and the spacer member and the strong adhesive layer adhere to each other. Preferably it is.
It is preferable that a measuring tank for measuring a certain amount of liquid is provided in the liquid channel, the closing part is provided at least upstream of the measuring tank, and the opening part is provided downstream.
It is preferable that the weighing tank is provided with an overflow portion for overflowing the liquid exceeding the certain amount.

　更に、本発明の液体流路装置は、基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、前記液体流路の一部を閉止状態から開通状態にする開通部を有し、前記開通部は、前記液体流路の前記一部に配置され、前記蓋板または前記液体流路の底部を外側から押圧する操作により塑性変形し、前記開通状態とする栓体からなることを特徴とする。
　前記蓋板または前記液体流路の底部における前記栓体と接する部分には、剥離処理が施されていることが好ましい。
　本発明の液体流路装置の製造方法は、前記液体流路装置の製造方法であって、　前記基板に前記液体流路と前記液槽とを形成する第１工程と、前記液体流路の前記一部に前記栓体を形成する第２工程と、前記基板の前記流路形成面に前記蓋板を積層する第３工程とを有し、前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記内層を構成するシートと、前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層することを特徴とする。
　前記第２工程では、前記液体流路の前記一部に、前記栓体を形成するための栓体形成材料を塗布する方法により、前記栓体を形成することが好ましい。 Furthermore, in the liquid flow path device of the present invention, a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate. A liquid flow path device in which a cover plate is laminated on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having an opening portion that changes a part of the liquid flow path from a closed state to an open state, and the opening portion Is a plug that is disposed in the part of the liquid flow path and is plastically deformed by an operation of pressing the lid plate or the bottom of the liquid flow path from the outside so as to be in the open state.
It is preferable that a peeling process is performed on a portion of the lid plate or the bottom portion of the liquid channel that contacts the plug.
The method for manufacturing a liquid channel device according to the present invention is a method for manufacturing the liquid channel device, wherein a first step of forming the liquid channel and the liquid tank on the substrate; A second step of partially forming the plug body and a third step of laminating the cover plate on the flow path forming surface of the substrate, wherein the first step constitutes an inner layer of the substrate An upper part of the liquid tank and the liquid channel are formed on a sheet, and a lower part of the liquid tank is formed on a sheet constituting the intermediate layer of the substrate, and then a sheet constituting the inner layer and the intermediate layer are formed. And a sheet constituting the outer layer of the substrate are sequentially laminated.
In the second step, it is preferable to form the plug body by a method of applying a plug body forming material for forming the plug body to the part of the liquid channel.

　更に、本発明の液体流路装置は、基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、前記液体流路の一部を開通状態から閉止状態にする閉止部を有し、前記閉止部は、前記液体流路の前記一部から分岐して形成された封止材料供給槽と、該封止材料供給槽に充填され、該封止材料供給槽に対応する部分の蓋板または該封止材料供給槽の底部を外側から押圧する操作により、前記液体流路の前記一部に押し出され、前記閉止状態とする封止材料と、を有することを特徴とする。
　本発明の液体流路装置の製造方法は、前記液体流路装置の製造方法であって、前記基板に前記液体流路と前記液槽と前記封止材料供給槽とを形成する第１工程と、前記封止材料供給槽に前記封止材料を充填する第２工程と、前記基板の前記流路形成面に前記蓋板を積層する第３工程とを有し、前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路と前記封止材料供給槽とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記内層を構成するシートと、前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層することを特徴とする。
　前記第２工程では、前記封止材料供給槽に前記封止材料を塗布する方法により、前記封止材料を充填することが好ましい。 Furthermore, in the liquid flow path device of the present invention, a liquid flow path through which liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate, and the liquid flow path of the substrate and the liquid flow path A liquid flow path device in which a cover plate is laminated on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having a closing portion that changes a part of the liquid flow path from an open state to a closed state, and the closing portion A sealing material supply tank formed by branching from the part of the liquid flow path, and a portion of the lid plate or the sealing member filled in the sealing material supply tank and corresponding to the sealing material supply tank. And a sealing material which is pushed out to the part of the liquid flow path by the operation of pressing the bottom portion of the stopping material supply tank from the outside to be in the closed state.
The method for manufacturing a liquid channel device of the present invention is a method for manufacturing the liquid channel device, wherein the liquid channel, the liquid tank, and the sealing material supply tank are formed on the substrate. And a second step of filling the sealing material supply tank with the sealing material, and a third step of laminating the lid plate on the flow path forming surface of the substrate. In the first step, After forming the upper part of the liquid tank, the liquid flow path and the sealing material supply tank on the sheet constituting the inner layer of the substrate, and forming the lower part of the liquid tank on the sheet constituting the intermediate layer of the substrate, The sheet constituting the inner layer, the sheet constituting the intermediate layer, and the sheet constituting the outer layer of the substrate are sequentially laminated.
In the second step, it is preferable to fill the sealing material by a method of applying the sealing material to the sealing material supply tank.

　更に、本発明の液体流路装置は、基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、前記液体流路の一部を閉止状態から開通状態にする開通部を有し、前記開通部は、前記液体流路の前記一部に配置された栓体を具備し、前記蓋板の内面または前記液体流路の底部における前記栓体の対向位置には、前記栓体を収納可能な凹部が構成され、前記栓体は、前記蓋板または前記底部を外側から押圧する操作により、前記液体流路の前記一部から前記凹部内に移動して、前記開通状態とすることを特徴とする。 Furthermore, in the liquid flow path device of the present invention, a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate. A liquid flow path device in which a cover plate is laminated on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having an opening portion that changes a part of the liquid flow path from a closed state to an open state, and the opening portion Includes a stopper disposed in the part of the liquid flow path, and a recessed portion capable of accommodating the plug body at an inner surface of the lid plate or a position opposite to the plug body on the bottom of the liquid flow path. The plug body is moved from the part of the liquid flow path into the recess by the operation of pressing the lid plate or the bottom part from the outside to be in the open state. .

　更に、本発明の液体流路装置の製造方法は、前記凹部が前記蓋板の前記内面に形成された液体流路装置の製造方法であって、前記基板に前記液体流路と前記液槽とを形成し、前記蓋板に前記凹部を形成する第１工程と、前記液体流路の前記一部に栓体を形成する第２工程と、前記基板の前記流路形成面に、前記蓋板を積層する第３工程とを有し、前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記基板の前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層して、前記基板に前記液体流路と前記液槽とを形成し、前記蓋板の内層を構成するシートに前記凹部を形成した後、前記蓋板の前記内層を構成するシートと、前記蓋板の外層を構成するシートとを積層して、前記蓋板に前記凹部を形成することを特徴とする。 Furthermore, the method for manufacturing a liquid channel device according to the present invention is a method for manufacturing a liquid channel device in which the concave portion is formed on the inner surface of the lid plate, and the liquid channel, the liquid tank, A first step of forming the recess in the lid plate, a second step of forming a plug in the part of the liquid channel, and the lid plate on the channel forming surface of the substrate In the first step, the upper part of the liquid tank and the liquid flow path are formed in a sheet constituting the inner layer of the substrate, and the sheet constituting the intermediate layer of the substrate The lower part of the liquid tank is formed on the sheet, the sheet constituting the inner layer of the substrate, the sheet constituting the intermediate layer of the substrate, and the sheet constituting the outer layer of the substrate are sequentially laminated, Forming the liquid flow path and the liquid tank on a substrate, forming a sheet constituting the inner layer of the lid plate After forming the serial recess, the sheet constituting the inner layer of the cover plate, by laminating the sheet constituting the outer layer of the cover plate, and forming the recess in the cover plate.

　また、本発明の液体流路装置の製造方法は、前記凹部が前記液体流路の前記底部に形成された液体流路装置の製造方法であって、前記基板に前記液体流路と前記液槽と前記凹部を形成する第１工程と、前記蓋板の内面の前記凹部の対向位置に、栓体を形成する第２工程と、前記基板の前記流路形成面に、前記蓋板を積層する第３工程とを有し、前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の内側中間層を構成するシートに前記液槽の中間部と前記凹部を形成し、前記基板の外側中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記内側中間層を構成するシートと、前記外側中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層することを特徴とする。 The method for manufacturing a liquid channel device of the present invention is a method for manufacturing a liquid channel device in which the concave portion is formed at the bottom of the liquid channel, and the liquid channel and the liquid tank are formed on the substrate. And a first step of forming the concave portion, a second step of forming a plug at a position of the inner surface of the lid plate facing the concave portion, and laminating the lid plate on the flow path forming surface of the substrate. A third step, and in the first step, the upper part of the liquid tank and the liquid flow path are formed in a sheet constituting the inner layer of the substrate, and the sheet constituting the inner intermediate layer of the substrate is formed in the sheet After forming the middle part of the liquid tank and the recess, and forming the lower part of the liquid tank on the sheet constituting the outer intermediate layer of the substrate, the sheet constituting the inner layer of the substrate and the inner intermediate layer Sheet, a sheet constituting the outer intermediate layer, and an outer layer of the substrate Characterized by sequentially laminating a that sheet.

　更に、本発明の液体流路装置は、基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、前記液体流路の一部を開通状態から閉止状態にする閉止部を有し、前記閉止部は、前記蓋板の内面または前記液体流路の底部に形成された凹部内に収納された栓体を具備し、前記栓体は、前記蓋板または前記底部を外側から押圧する操作により前記凹部内から前記液体流路の前記一部に移動して、前記閉止状態とすることを特徴とする。 Furthermore, in the liquid flow path device of the present invention, a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate. A liquid flow path device in which a cover plate is stacked on a flow path forming surface in which a liquid tank is formed, the liquid flow path device having a closing portion that changes a part of the liquid flow path from an open state to a closed state, and the closing portion Comprises a stopper housed in a recess formed in the inner surface of the lid plate or the bottom of the liquid flow path, and the stopper body is formed by pressing the lid plate or the bottom portion from outside. It moves to the said part of the said liquid flow path from the inside, It is set as the said closed state.

　本発明の液体流路装置の製造方法は、前記凹部が前記蓋板の前記内面に形成された液体流路装置の製造方法であって、前記基板に前記液体流路と前記液槽とを形成し、前記蓋板に前記凹部を形成する第１工程と、前記凹部内に栓体を形成する第２工程と、前記基板の前記流路形成面に、前記蓋板を積層する第３工程とを有し、前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記基板の前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層して、前記基板に前記液体流路と前記液槽とを形成し、前記蓋板の内層を構成するシートに前記凹部を形成した後、前記蓋板の前記内層を構成するシートと、前記蓋板の外層を構成するシートとを積層して、前記蓋板に前記凹部を形成することを特徴とする。 The method for manufacturing a liquid channel device of the present invention is a method for manufacturing a liquid channel device in which the recess is formed on the inner surface of the lid plate, and the liquid channel and the liquid tank are formed on the substrate. A first step of forming the recess in the lid plate, a second step of forming a plug in the recess, and a third step of laminating the lid plate on the flow path forming surface of the substrate, In the first step, an upper part of the liquid tank and the liquid channel are formed in a sheet constituting the inner layer of the substrate, and a lower part of the liquid tank is formed in the sheet constituting the intermediate layer of the substrate. After forming, the sheet constituting the inner layer of the substrate, the sheet constituting the intermediate layer of the substrate, and the sheet constituting the outer layer of the substrate are sequentially laminated, and the liquid flow path is formed on the substrate. The liquid tank was formed, and the recess was formed in a sheet constituting the inner layer of the lid plate , A sheet constituting the inner layer of the cover plate, by laminating the sheet constituting the outer layer of the cover plate, and forming the recess in the cover plate.

　また、本発明の液体流路装置の製造方法は、前記凹部が前記液体流路の底部に形成された液体流路装置の製造方法であって、前記基板に前記液体流路と前記液槽と前記凹部を形成する第１工程と、前記凹部内に栓体を形成する第２工程と、前記基板の前記流路形成面に前記蓋板を積層する第３工程とを有し、前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の内側中間層を構成するシートに前記液槽の中間部と前記凹部を形成し、前記基板の外側中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記内側中間層を構成するシートと、前記外側中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層することを特徴とする。
　前記各第２工程では、前記栓体を形成するための栓体形成材料を塗布する方法により、前記栓体を形成することが好適である。 The method for manufacturing a liquid channel device of the present invention is a method for manufacturing a liquid channel device in which the concave portion is formed at the bottom of the liquid channel, and the liquid channel, the liquid tank, A first step of forming the concave portion, a second step of forming a plug in the concave portion, and a third step of laminating the lid plate on the flow path forming surface of the substrate. In the process, an upper part of the liquid tank and the liquid flow path are formed in a sheet constituting the inner layer of the substrate, and an intermediate part and the concave part of the liquid tank are formed in a sheet constituting the inner intermediate layer of the substrate. After forming the lower part of the liquid tank on the sheet constituting the outer intermediate layer of the substrate, the sheet constituting the inner layer of the substrate, the sheet constituting the inner intermediate layer, and the outer intermediate layer are formed. A sheet and a sheet constituting the outer layer of the substrate are sequentially laminated. To.
In each of the second steps, it is preferable that the plug is formed by a method of applying a plug forming material for forming the plug.

　本発明によれば、液体を流通させるための装置を別途必要とすることなく、簡便に液体流路を閉止状態から開通状態にでき、即ち液体を簡便かつ円滑に流路に流通させることのできる液体流路装置を低コストで提供できる。
　また、本発明によれば、簡便に液体流路を閉止状態から開通状態、開通状態から閉止状態に、または開通状態から閉止状態にしたり閉止状態から開通状態にできる液体流路装置を低コストで提供できる。 According to the present invention, the liquid channel can be easily changed from the closed state to the open state without requiring a separate device for circulating the liquid, that is, the liquid can be easily and smoothly circulated through the channel. A liquid channel device can be provided at low cost.
Further, according to the present invention, a liquid flow path device that can easily change the liquid flow path from the closed state to the open state, from the open state to the closed state, or from the open state to the closed state, or from the closed state to the open state, at low cost. Can be provided.

第１実施形態の液体流路装置を示す概略平面透視図である。It is a schematic plane perspective view showing the liquid channel device of a 1st embodiment. 図１の液体流路装置の一部を拡大した平面透視図である。FIG. 2 is an enlarged plan perspective view of a part of the liquid channel device in FIG. 1. 図２のＩ－Ｉ’線に沿う断面図である。FIG. 3 is a cross-sectional view taken along the line I-I ′ of FIG. 2. 図１の液体流路装置において、開通部が作動する様子を説明する説明図である。FIG. 2 is an explanatory diagram for explaining a state in which an opening portion operates in the liquid channel device of FIG. 1. 図１の液体流路装置において、開通部が作動する様子を説明する説明図である。FIG. 2 is an explanatory diagram for explaining a state in which an opening portion operates in the liquid channel device of FIG. 1. 図１の液体流路装置において、閉止部が作動する様子を説明する説明図である。FIG. 2 is an explanatory diagram for explaining a state in which a closing portion operates in the liquid channel device of FIG. 1. 図１の液体流路装置において、閉止部が作動する様子を説明する説明図である。FIG. 2 is an explanatory diagram for explaining a state in which a closing portion operates in the liquid channel device of FIG. 1. 図１の液体流路装置において、送液部が作動する様子を説明する説明図である。FIG. 2 is an explanatory diagram for explaining a state in which a liquid feeding section operates in the liquid channel device of FIG. 1. 図１の液体流路装置において、送液部が作動する様子を説明する説明図である。FIG. 2 is an explanatory diagram for explaining a state in which a liquid feeding section operates in the liquid channel device of FIG. 1. 図１の液体流路装置の基板の一部を拡大した斜視図である。FIG. 2 is an enlarged perspective view of a part of a substrate of the liquid channel device in FIG. 1. 本発明の液体流路装置の第２実施形態を示す概略平面透視図である。It is a schematic plane perspective view which shows 2nd Embodiment of the liquid flow-path apparatus of this invention. 図８の液体流路装置の使用方法の一例を説明する概略説明図である。It is a schematic explanatory drawing explaining an example of the usage method of the liquid flow-path apparatus of FIG. 第３実施形態の液体流路装置において、送液部が作動する様子を説明する説明図である。In the liquid channel apparatus of 3rd Embodiment, it is explanatory drawing explaining a mode that a liquid feeding part act | operates. 第３実施形態の液体流路装置において、送液部が作動する様子を説明する説明図である。In the liquid channel apparatus of 3rd Embodiment, it is explanatory drawing explaining a mode that a liquid feeding part act | operates. 図１０Ａ，１０Ｂの液体流路装置の基板の一部を拡大した斜視図である。It is the perspective view which expanded some substrates of the liquid channel device of Drawing 10A and 10B. 図１０Ａ，１０Ｂの液体流路装置の基板の製造工程を示す概略図である。It is the schematic which shows the manufacturing process of the board | substrate of the liquid flow-path apparatus of FIG. 10A and 10B. 第３実施形態の液体流路装置の基板について、他の形態を示す断面図である。It is sectional drawing which shows another form about the board | substrate of the liquid flow-path apparatus of 3rd Embodiment. 本発明の第４実施形態の液体流路装置を示す概略平面透視図である。It is a schematic plane perspective view which shows the liquid channel apparatus of 4th Embodiment of this invention. 図１４の液体流路装置の一部を拡大した平面透視図である。FIG. 15 is a plan perspective view in which a part of the liquid channel device in FIG. 14 is enlarged. 図１５のＩ－Ｉ’線に沿う断面図である。FIG. 16 is a cross-sectional view taken along the line I-I ′ of FIG. 15. 図１４の液体流路装置において、開通部が作動する様子を説明する図であって、栓体に荷重を加えた状態を示す断面図である。In the liquid channel device of FIG. 14, it is a figure explaining a mode that an opening part act | operates, Comprising: It is sectional drawing which shows the state which applied the load to the stopper. 図１４の液体流路装置において、開通部が作動する様子を説明する図であって、荷重を取り去った状態を示す断面図である。In the liquid channel device of FIG. 14, it is a figure explaining a mode that an opening part act | operates, Comprising: It is sectional drawing which shows the state which removed the load. 図１５のＩＩ－ＩＩ’線に沿う断面図である。FIG. 16 is a cross-sectional view taken along the line II-II ′ of FIG. 15. 図１４の液体流路装置において、閉止部が作動する様子を説明する図であって、封止材料を押し出した状態を示す断面図とである。In the liquid channel device of FIG. 14, it is a figure explaining a mode that a closing part act | operates, Comprising: It is sectional drawing which shows the state which extruded the sealing material. 図１４の液体流路装置において、閉止部が作動する様子を説明する図であって、平面図である。In the liquid channel apparatus of FIG. 14, it is a figure explaining a mode that a closing part act | operates, Comprising: It is a top view. 図１４の液体流路装置の製造方法を模式的に説明する工程図である。FIG. 15 is a process diagram schematically illustrating a manufacturing method of the liquid channel device in FIG. 14. 第５実施形態の液体流路装置を示す概略平面透視図である。It is a schematic plane perspective view which shows the liquid channel apparatus of 5th Embodiment. 図２１の液体流路装置の一部を拡大した平面透視図である。FIG. 22 is an enlarged plan perspective view of a part of the liquid channel device of FIG. 21. 図２２のＩ－Ｉ’線に沿う断面図である。FIG. 23 is a cross-sectional view taken along the line I-I ′ of FIG. 22. 図２３中の開通部の拡大断面図である。It is an expanded sectional view of the opening part in FIG. 図２３中の閉止部の拡大断面図である。It is an expanded sectional view of the closing part in FIG. 図２１の液体流路装置において、開通部が作動する様子を説明する図であって、蓋板を外側から押圧して荷重を加えた状態を示す断面図である。In the liquid channel device of FIG. 21, it is a figure explaining a mode that an opening part act | operates, Comprising: It is sectional drawing which shows the state which pressed the cover plate from the outer side and added the load. 図２１の液体流路装置において、開通部が作動する様子を説明する図であって、荷重を取り去った状態を示す断面図である。In the liquid channel device of FIG. 21, it is a figure explaining a mode that an opening part act | operates, Comprising: It is sectional drawing which shows the state which removed the load. 図２１の液体流路装置において、閉止部が作動する様子を説明する図であって、蓋板を外側から押圧して荷重を加えた状態を示す断面図である。In the liquid channel device of FIG. 21, it is a figure explaining a mode that a closing part act | operates, Comprising: It is sectional drawing which shows the state which pressed the cover plate from the outer side and added the load. 図２１の液体流路装置において、閉止部が作動する様子を説明する図であって、荷重を取り去った状態を示す断面図である。FIG. 22 is a diagram illustrating a state in which the closing portion operates in the liquid channel device of FIG. 21, and is a cross-sectional view illustrating a state where a load is removed. 図２１の液体流路装置の製造方法を模式的に説明する工程図である。FIG. 22 is a process diagram schematically illustrating a manufacturing method of the liquid channel device in FIG. 21. 図２１の液体流路装置において、栓体受けが設けられた状態を示す断面図である。FIG. 22 is a cross-sectional view showing a state in which a stopper receiver is provided in the liquid channel device of FIG. 21. 栓体の平面視形状を菱形にした例を模式的に示す平面図である。It is a top view which shows typically the example which made the planar view shape of the plug body the rhombus. 第６実施形態の液体流路装置について、その一部を示す断面図である。It is sectional drawing which shows the part about the liquid flow-path apparatus of 6th Embodiment. 図３０中の開通部の拡大断面図である。It is an expanded sectional view of the opening part in FIG. 図３０中の閉止部の拡大断面図である。It is an expanded sectional view of the closing part in FIG. 図３０の液体流路装置において、開通部が作動する様子を説明する図であって、蓋板を外側から押圧して荷重を加えた状態を示す断面図である。In the liquid channel device of FIG. 30, it is a figure explaining a mode that an opening part act | operates, Comprising: It is sectional drawing which shows the state which pressed the cover plate from the outer side and added the load. 図３０の液体流路装置において、開通部が作動する様子を説明する図であって、荷重を取り去った状態を示す断面図である。In the liquid channel device of FIG. 30, it is a figure explaining a mode that an opening part act | operates, Comprising: It is sectional drawing which shows the state which removed the load. 図３０の液体流路装置において、閉止部が作動する様子を説明する図であって、蓋板を外側から押圧して荷重を加えた状態を示す断面図である。In the liquid channel device of FIG. 30, it is a figure explaining a mode that a closing part act | operates, Comprising: It is sectional drawing which shows the state which pressed the cover plate from the outer side and added the load. 図３０の液体流路装置において、閉止部が作動する様子を説明する図であって、荷重を取り去った状態を示す断面図である。In the liquid channel device of FIG. 30, it is a figure explaining a mode that a closing part act | operates, Comprising: It is sectional drawing which shows the state which removed the load. 図３０の液体流路装置の製造方法を模式的に説明する工程図である。FIG. 31 is a process diagram schematically illustrating a manufacturing method of the liquid channel device in FIG. 30.

　以下、本発明について詳細に説明する。
［第１実施形態］
　図１は第１実施形態の液体流路装置１０Ａを概略的に示す平面透視図、図２は図１の液体流路装置１０Ａの一部を拡大した平面透視図、図３は図２のＩ－Ｉ’線に沿う断面図である。
　この液体流路装置１０Ａは、平板からなる四角形の基板１１Ａの片面に、試料および試薬の少なくとも一方からなる液体が流通する溝状の液体流路１２と、液体流路１２の端部や途中において液体が溜まる複数（この例では９）の液槽（１４ａ～１４ｉ）とが形成され、基板１１Ａの液体流路１２と液槽（１４ａ～１４ｉ）とが形成された側の流路形成面１２ａに、蓋板１３が積層している。
　この例の液体流路装置１０Ａは、図１中の上端部側が上方に、下端部側が下方に位置するように立てられただけでも、液体流路１２の上流側の端部から下流側の端部に向けて矢印Ｆ方向に試料が重力により流通し、その途中で試料に対して各種の処理や試薬との混合がなされ、各種検出、分析に供される測定液が調製される。しかしながら、この例では、詳しくは後述するように、液槽の有する送液部も併用することによって、液体を簡便かつ円滑に流通させ得るようになっている。 Hereinafter, the present invention will be described in detail.
[First Embodiment]
FIG. 1 is a plan perspective view schematically showing the liquid channel device 10A of the first embodiment, FIG. 2 is a plan perspective diagram showing an enlarged part of the liquid channel device 10A of FIG. 1, and FIG. FIG. 6 is a cross-sectional view taken along the line −I ′.
This liquid flow path device 10A includes a groove-shaped liquid flow path 12 through which a liquid consisting of at least one of a sample and a reagent circulates on one side of a flat substrate 11A made of a flat plate, and an end portion or a middle of the liquid flow path 12 A plurality of (9 in this example) liquid tanks (14a to 14i) in which liquid is accumulated are formed, and a flow path forming surface 12a on the side where the liquid flow path 12 and the liquid tanks (14a to 14i) of the substrate 11A are formed. In addition, a cover plate 13 is laminated.
Even if the liquid channel device 10A of this example is erected so that the upper end side in FIG. 1 is located above and the lower end side is located below, the end on the downstream side from the upstream end of the liquid channel 12 The sample circulates in the direction of arrow F toward the portion by gravity, and the sample is mixed with various treatments and reagents in the middle of the sample to prepare a measurement solution for various detections and analyses. However, in this example, as will be described in detail later, the liquid can be easily and smoothly circulated by using the liquid feeding part of the liquid tank in combination.

　液体流路１２の上流側の端部には、投入された試料が溜まる試料投入槽１４ａが設けられ、この試料投入槽１４ａの下流には、試料投入槽１４ａから流通してきた試料に対してろ過処理が施される図示略のフィルタが内蔵されたろ過槽１４ｂが設けられている。
ろ過槽１４ｂの下流には、その内容積が所定量に形成され、ろ過処理された試料を計量できる計量槽１４ｃが設けられている。 At the upstream end of the liquid channel 12, there is provided a sample loading tank 14a in which the loaded sample is collected, and the sample flowing from the sample loading tank 14a is filtered downstream of the sample loading tank 14a. A filtration tank 14b containing a filter (not shown) to be processed is provided.
Downstream of the filtration tank 14b, there is provided a measuring tank 14c whose inner volume is formed in a predetermined amount and capable of weighing the filtered sample.

　この例の計量槽１４ｃには、オーバーフロー流路１２ｂとその下流に設けられた廃液槽１４ｄとからなるオーバーフロー部が備えられている。そのため、計量槽１４ｃで一定量を超えた試料はオーバーフローしてオーバーフロー流路１２ｂを流れ、廃液槽１４ｄに流入し、その結果、計量槽１４ｃでは、一定量の試料が計量できる。 The weighing tank 14c in this example is provided with an overflow portion composed of an overflow channel 12b and a waste liquid tank 14d provided downstream thereof. Therefore, the sample exceeding a certain amount in the measuring tank 14c overflows and flows through the overflow channel 12b and flows into the waste liquid tank 14d. As a result, a certain amount of sample can be measured in the measuring tank 14c.

　計量槽１４ｃの下流には、計量槽１４ｃで計量された試料と、あらかじめ第１試薬槽１４ｅに所定量封入されている液体の第１試薬とが混合される第１混合槽１４ｆが設けられ、第１混合槽１４ｆの下流には、第１混合槽１４ｆで調製された中間調製液と、あらかじめ第２試薬槽１４ｇに所定量封入されている液体の第２試薬とが混合される第２混合槽１４ｈが設けられている。
そして、第２混合槽１４ｈの下流には測定槽１４ｉが設けられ、第２混合槽１４ｈで調製された測定液が測定槽１４ｉに貯留され、図示略の検出分析部により、各種成分の検出や分析が行われる。
なお、この例では、測定槽１４ｉの下流に、測定槽１４ｉと液体流路１２により連通した混合機能槽１４ｄが設けられている。詳しくは後述するが、測定槽１４ｉ内の測定液を混合機能槽１４ｄに一旦送った後、測定槽１４ｉに返送する混合操作を必要に応じて繰り返すことにより、測定液が十分に攪拌混合され、より検出や分析に適した状態になる。
また、各液槽には、大気と連通する開閉可能な図示略の連通孔が設けられている。 Downstream of the measuring tank 14c, a first mixing tank 14f is provided in which the sample weighed in the measuring tank 14c and the liquid first reagent previously sealed in the first reagent tank 14e are mixed. Downstream of the first mixing tank 14f is a second mixing in which the intermediate preparation liquid prepared in the first mixing tank 14f and the liquid second reagent sealed in a predetermined amount in the second reagent tank 14g are mixed. A tank 14h is provided.
A measuring tank 14i is provided downstream of the second mixing tank 14h, and the measurement liquid prepared in the second mixing tank 14h is stored in the measuring tank 14i. Analysis is performed.
In this example, a mixing function tank 14d communicating with the measurement tank 14i and the liquid channel 12 is provided downstream of the measurement tank 14i. As will be described in detail later, the measurement liquid is sufficiently stirred and mixed by repeating the mixing operation of returning the measurement liquid in the measurement tank 14i to the mixing function tank 14d and then returning to the measurement tank 14i as necessary. It becomes a state more suitable for detection and analysis.
Each liquid tank is provided with a communication hole (not shown) that can be opened and closed and communicates with the atmosphere.

　この液体流路装置１０Ａの蓋板１３は、図３に示すように、蓋板１３の表面を構成する第１基材層１３ａと、第１基材層１３ａの内側に形成された強粘着層１３ｂと、強粘着層１３ｂの内側に形成された第２基材層１３ｃと、第２基材層１３ｃの内側に形成され、流路形成面１２ａに粘着する弱粘着層１３ｄとを有している。
　第１基材層１３ａは、表面側から垂直方向（第１基材層１３ａと垂直に交差する方向）の荷重が加えられた場合には撓み、その後、荷重が取り去られた場合には元に戻る復元力を有する材料からなっている。一方、第２基材層１３ｃは、同様の荷重により容易に撓み、荷重を取り去っても復元しない、すなわち容易に塑性変形する材料からなっている。また、強粘着層１３ｂの粘着力は、弱粘着層１３ｄよりも大きく形成されている。 As shown in FIG. 3, the lid plate 13 of the liquid flow path device 10A includes a first base layer 13a constituting the surface of the lid plate 13, and a strong adhesive layer formed on the inner side of the first base layer 13a. 13b, a second base material layer 13c formed inside the strong adhesive layer 13b, and a weak adhesive layer 13d formed inside the second base material layer 13c and sticking to the flow path forming surface 12a. Yes.
The first base material layer 13a bends when a load in the vertical direction (a direction perpendicular to the first base material layer 13a) is applied from the surface side, and then returns to the original when the load is removed. It is made of a material that has a restoring force to return to. On the other hand, the second base material layer 13c is made of a material that is easily bent by the same load and does not recover even when the load is removed, that is, easily plastically deformed. Moreover, the adhesive force of the strong adhesion layer 13b is formed larger than the weak adhesion layer 13d.

　また、この液体流路装置１０Ａは、液体流路１２の一部を閉止状態から開通状態にする開通部Ｓ１～Ｓ７と、開通状態から閉止状態にする閉止部Ｔ１とを有している。
この例では、開通部Ｓ１～Ｓ７は、試料投入槽１４ａとろ過槽１４ｂとの間、ろ過槽１４ｂと計量槽１４ｃとの間、計量槽１４ｃと第１混合槽１４ｆとの間、第１混合槽１４ｆと第２混合槽１４ｈとの間、第１試薬槽１４ｅと第１混合槽１４ｆとの間、第２試薬槽１４ｇと第２混合槽１４ｈとの間、第２混合槽１４ｈと測定槽１４ｉとの間の各液体流路１２にそれぞれ１ずつ設けられている。
　一方、閉止部Ｔ１は、ろ過槽１４ｂと計量槽１４ｃとの間の液体流路１２において、開通部Ｓ２よりも下流側に設けられている。 Further, the liquid channel device 10A includes opening portions S1 to S7 that make a part of the liquid channel 12 from the closed state to the open state, and a closing portion T1 that changes the open state to the closed state.
In this example, the opening sections S1 to S7 are provided between the sample feeding tank 14a and the filtration tank 14b, between the filtration tank 14b and the weighing tank 14c, between the weighing tank 14c and the first mixing tank 14f, Between the tank 14f and the second mixing tank 14h, between the first reagent tank 14e and the first mixing tank 14f, between the second reagent tank 14g and the second mixing tank 14h, and between the second mixing tank 14h and the measuring tank One is provided in each liquid flow path 12 between 14i.
On the other hand, the closing part T1 is provided on the downstream side of the opening part S2 in the liquid flow path 12 between the filtration tank 14b and the measuring tank 14c.

　そして、各開通部Ｓ１～Ｓ７においては、図３のＳ１およびＳ２を例示して説明すると、液体流路１２に第１凸部１５が形成され、この第１凸部１５の頂部１５ａと弱粘着層１３ｄとが粘着し、かつ、強粘着層１３ｂと第２基材層１３ｃとが離間している。
　よって、各開通部Ｓ１～Ｓ７における液体流路１２は、第１凸部１５とこれの頂部１５ａに粘着した弱粘着層１３ｄとにより閉じられ、通常時は閉止状態となっている。ところが、図４Ａ，４Ｂに開通部Ｓ１を例に挙げて示すように、この開通部Ｓ１おける第１基材層１３ａを表面側から矢印Ａで示すように押圧して、第１基材層１３ａに垂直方向の荷重を加えた場合には、図４Ａに示すように、第１基材層１３ａが撓み、第１基材層１３ａの内側の強粘着層１３ｂが第２基材層１３ｃに粘着する。そして、その後に荷重を取り去ると、図４Ｂに示すように、第１基材層１３ａはその復元力により元の状態に復元し、その際、第１基材層１３ａの内側に粘着した強粘着層１３ｂと、強粘着層１３ｂに粘着し、容易に塑性変形可能な第２基材層１３ｃと、第２基材層１３ｃの内側に粘着した弱粘着層１３ｄも第１基材層１３ａの復元に追従し、持ち上がる。その結果、第１凸部１５の頂部１５ａと弱粘着層１３ｄとの間が新たに離間し、ここを液体が流通する。
　このように開通部Ｓ１～Ｓ７においては、蓋板１３を表面側から押圧して垂直方向の荷重を加えた後、この荷重を取り去る押圧操作によって、元々は粘着していた第１凸部１５の頂部１５ａと弱粘着層１３ｄとの間が離間し、その結果、この部分の液体流路１２が閉止状態から開通状態となる。 Then, in each of the opening portions S1 to S7, to explain with reference to S1 and S2 of FIG. 3, the first convex portion 15 is formed in the liquid flow path 12, and the top portion 15a of the first convex portion 15 is weakly adhered. The layer 13d adheres, and the strong adhesion layer 13b and the second base material layer 13c are separated from each other.
Therefore, the liquid flow path 12 in each of the opening portions S1 to S7 is closed by the first convex portion 15 and the weak adhesive layer 13d adhered to the top portion 15a, and is normally closed. However, as shown in FIGS. 4A and 4B by taking the opening portion S1 as an example, the first base material layer 13a in the opening portion S1 is pressed from the surface side as indicated by the arrow A, and the first base material layer 13a is pressed. When a vertical load is applied to the first base material layer 13a, the first base material layer 13a bends and the strong adhesive layer 13b inside the first base material layer 13a sticks to the second base material layer 13c, as shown in FIG. 4A. To do. Then, when the load is removed thereafter, as shown in FIG. 4B, the first base material layer 13a is restored to its original state by its restoring force, and at that time, the strong adhesive sticking to the inside of the first base material layer 13a. The second base material layer 13c that adheres to the layer 13b, the strong adhesive layer 13b, and can be easily plastically deformed, and the weak adhesive layer 13d that adheres to the inside of the second base material layer 13c are also restored to the first base material layer 13a. Follow and lift up. As a result, the top portion 15a of the first convex portion 15 and the weak adhesive layer 13d are newly separated, and the liquid flows therethrough.
As described above, in the opening portions S1 to S7, the cover plate 13 is pressed from the surface side to apply a load in the vertical direction, and then the pressing operation for removing the load removes the load from the first convex portion 15 that was originally adhered. The top portion 15a and the weak adhesive layer 13d are separated from each other, and as a result, the liquid channel 12 in this portion is changed from the closed state to the open state.

　一方、閉止部Ｔ１においては、図３に示すように、液体流路１２に第２凸部１６が形成され、この第２凸部１６の頂部１６ａと弱粘着層１３ｄとは離間し、かつ、強粘着層１３ｂと第２基材層１３ｃとの間にはスペーサ部材１７が介在し、スペーサ部材１７と強粘着層１３ｂとが粘着している。
　よって、閉止部Ｔ１における液体流路１２では、第２凸部１６の頂部１６ａと弱粘着層１３ｄとの間が離間して流路が保たれ、通常時は開通状態である。ところが、図５Ａに示すように、閉止部Ｔ１における第１基材層１３ａを表面側から矢印Ｂで示すように押圧して、第１基材層１３ａに垂直方向の荷重を加えた場合には、第１基材層１３ａが撓み、その結果、蓋板１３の内層の弱粘着層１３ｄが第２凸部１６の頂部１６ａに粘着する。そして、その後に荷重を取り去ると、図５Ｂに示すように、第１基材層１３ａはその復元力により元の状態に復元し、その際、第１基材層１３ａの内側に粘着した強粘着層１３ｂと、強粘着層１３ｂに粘着したスペーサ部材１７は、第１基材層１３ａの復元に追従して持ち上がる。一方、スペーサ部材１７と第２基材層１３ｃとの間は粘着していないとともに、第２基材層１３ｃは容易に塑性変形可能であるために、ここで荷重を取り去っても、第２基材層１３ｃと弱粘着層１３ｄは第１基材層１３ａの復元には追従しない。その結果、第２凸部１６の頂部１６ａと弱粘着層１３ｄとは粘着した状態となって液体流路１２を閉止し、液体はここを流通できなくなる。
　このように閉止部Ｔ１においては、蓋板１３を表面側から押圧して垂直方向の荷重を加えた後、この荷重を取り去る押圧操作によって、元々は離間していた第２凸部１６の頂部１６ａと弱粘着層１３ｄとの間が粘着して閉塞し、その結果、この部分の液体流路１２が開通状態から閉止状態となる。 On the other hand, in the closing portion T1, as shown in FIG. 3, a second convex portion 16 is formed in the liquid flow path 12, and the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are separated from each other, and A spacer member 17 is interposed between the strong adhesion layer 13b and the second base material layer 13c, and the spacer member 17 and the strong adhesion layer 13b are adhered.
Therefore, in the liquid flow path 12 in the closing part T1, the top part 16a of the 2nd convex part 16 and the weak adhesion layer 13d are separated, the flow path is maintained, and it is an open state normally. However, as shown in FIG. 5A, when a load in the vertical direction is applied to the first base material layer 13a by pressing the first base material layer 13a in the closing portion T1 as indicated by an arrow B from the surface side. The first base material layer 13a bends, and as a result, the weak adhesive layer 13d of the inner layer of the cover plate 13 adheres to the top 16a of the second convex portion 16. Then, when the load is removed thereafter, as shown in FIG. 5B, the first base material layer 13a is restored to its original state by its restoring force, and at that time, the strong adhesive sticking to the inside of the first base material layer 13a. The spacer member 17 adhered to the layer 13b and the strong adhesion layer 13b is lifted following the restoration of the first base material layer 13a. On the other hand, the spacer member 17 is not adhered between the second base material layer 13c and the second base material layer 13c can be easily plastically deformed. The material layer 13c and the weak adhesive layer 13d do not follow the restoration of the first base material layer 13a. As a result, the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are in an adhesive state, and the liquid channel 12 is closed, so that the liquid cannot flow therethrough.
As described above, in the closing portion T1, the top plate 16a of the second convex portion 16 that was originally separated by the pressing operation of removing the load after pressing the cover plate 13 from the surface side and applying a vertical load. And the weak adhesive layer 13d are adhered and closed, and as a result, the liquid flow path 12 in this portion is changed from the open state to the closed state.

　さらに、この例の液体流路装置１０Ａでは、計量槽１４ｃと、第１混合槽１４ｆと、第２混合槽１４ｈと、測定槽１４ｉと、混合機能槽１４ｄは、各液槽内の液体を液槽外に送液する送液部Ｐ１～Ｐ５をそれぞれ有している。
　これら送液部Ｐ１～Ｐ５のうち、計量槽１４ｃの送液部Ｐ１は、計量槽１４ｃ内の液体を下流側、すなわち第１混合槽１４ｆへと送液する。同様に、第１混合槽１４ｆの送液部Ｐ２は、第１混合槽１４ｆ内の液体を下流側、すなわち第２混合槽１４ｈへと送液する。第２混合槽１４ｈの送液部Ｐ３は、第２混合槽１４ｈ内の液体を下流側、すなわち測定槽１４ｉへと送液する。また、測定槽１４ｉの送液部Ｐ４は、測定槽１４ｉ内の液体を下流側、すなわち混合機能槽１４ｄへと送液する。
　一方、混合機能槽１４ｄの送液部Ｐ５は、混合機能槽１４ｄ内の液体を上流側、すなわち測定槽１４ｉへと返送する。 Furthermore, in the liquid channel device 10A of this example, the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, the measuring tank 14i, and the mixing function tank 14d are configured to liquidate liquid in each liquid tank. Each has liquid feeding parts P1 to P5 for feeding liquid outside the tank.
Among these liquid feeding parts P1 to P5, the liquid feeding part P1 of the measuring tank 14c sends the liquid in the measuring tank 14c to the downstream side, that is, the first mixing tank 14f. Similarly, the liquid feeding part P2 of the first mixing tank 14f feeds the liquid in the first mixing tank 14f to the downstream side, that is, the second mixing tank 14h. The liquid feeding part P3 of the second mixing tank 14h feeds the liquid in the second mixing tank 14h to the downstream side, that is, the measurement tank 14i. Moreover, the liquid feeding part P4 of the measuring tank 14i sends the liquid in the measuring tank 14i to the downstream side, that is, the mixing function tank 14d.
On the other hand, the liquid feeding part P5 of the mixing function tank 14d returns the liquid in the mixing function tank 14d to the upstream side, that is, the measurement tank 14i.

　そして、この例では、各送液部Ｐ１～Ｐ５を有する各液槽に対応する部分の蓋板１３（各液槽を閉塞する部分の蓋板）においては、強粘着層１３ｂと第２基材層１３ｃとの間は離間しているのではなく、スペーサ部材１７が介在して、スペーサ部材１７と強粘着層１３ｂとが粘着し、層間が密に構成されている。
　そのため、送液部Ｐ１を例に挙げて図６Ａ，６Ｂに示すように、この部分の蓋板１３を外側から矢印Ｃで示す方向に押圧した場合（図６Ｂ）、押圧された部分の蓋板１３は内側に撓む。その結果、計量槽１４ｃの内容積が小さくなり、計量槽１４ｃ内の液体が吐出されて送液され、送液部Ｐ１としての作用が発現する。ここで仮に、強粘着層１３ｂと第２基材層１３ｃとの間が離間し、スペーサ部材１７が介在しておらず、層間が密でないと、この部分の蓋板１３を外側から押圧しても、強粘着層１３ｂが第２基材層１３ｃに粘着するだけで、計量槽１４ｃの内容積が小さくならない可能性がある。その場合、送液部としての作用は発現しない。 In this example, in the lid plate 13 corresponding to each liquid tank having the liquid feeding parts P1 to P5 (the lid plate covering the liquid tank), the strong adhesive layer 13b and the second substrate The spacer 13 is not spaced apart from the layer 13c, but the spacer member 17 and the strong adhesion layer 13b are adhered to each other, and the layers are densely formed.
Therefore, as shown in FIGS. 6A and 6B by taking the liquid feeding part P1 as an example, when the lid plate 13 of this portion is pressed in the direction indicated by the arrow C from the outside (FIG. 6B), the lid plate of the pressed portion. 13 bends inward. As a result, the internal volume of the measuring tank 14c becomes small, the liquid in the measuring tank 14c is discharged and fed, and the action as the liquid feeding part P1 is expressed. Here, if the strong adhesion layer 13b and the second base material layer 13c are separated from each other, the spacer member 17 is not interposed, and the interlayer is not dense, the cover plate 13 of this portion is pressed from the outside. However, there is a possibility that the internal volume of the measuring tank 14c is not reduced only by the strong adhesion layer 13b sticking to the second base material layer 13c. In that case, the action as a liquid feeding part does not appear.

　また、この例では、このような送液部Ｐ１～Ｐ４がそれぞれ設けられた計量槽１４ｃと、第１混合槽１４ｆと、第２混合槽１４ｈと、測定槽１４ｉとには、図１および図２に示すように、送液部Ｐ１～Ｐ４で送液される液体の上流側への逆流を防止する逆流防止部Ｇ１～Ｇ６も設けられている。そのため、送液部Ｐ１～Ｐ４を作動させた際に、各液槽内の液体は上流側には逆流せず、下流側にのみ送液される。 Further, in this example, the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, and the measuring tank 14i each provided with such liquid feeding sections P1 to P4 are shown in FIGS. As shown in FIG. 2, there are also provided backflow prevention units G1 to G6 for preventing the backflow of the liquid fed by the liquid feeding units P1 to P4 to the upstream side. Therefore, when the liquid feeding sections P1 to P4 are operated, the liquid in each liquid tank does not flow backward on the upstream side but is fed only on the downstream side.

　逆流防止部Ｇ１～Ｇ６は、この例では、可撓性のある堰板１８から構成されている。
　例えば、計量槽１４ｃを例示すると、堰板１８は、図６Ａ、図６Ｂおよび図７に示すように、計量槽１４ｃと計量槽１４ｃの上流側の液体流路１２との境界部分において、堰板１８の先端１８ａが下流側に傾くように、基端１８ｂのみが液体流路１２の底部に固定されていて、先端１８ａや両側端は固定されていない。
　そのため、図６Ａ、図６Ｂおよび図７では図示略のろ過槽から計量槽１４ｃへ液体が送液される場合には、液体は堰板１８の先端１８ａを超えて計量槽１４ｃへと流入することができる。
一方、計量槽１４ｃの有する送液部Ｐ１が作動し、計量槽１４ｃの内容積が小さくなった場合には、このような堰板１８が配置されているために、図６Ｂに示すように、計量槽１４ｃ内の液体は下流側にしか送液されず、上流側、すなわちろ過槽側へは逆流しない。 In this example, the backflow prevention parts G1 to G6 are constituted by a flexible weir plate 18.
For example, when the measuring tank 14c is illustrated, as shown in FIGS. 6A, 6B, and 7, the weir plate 18 is a weir plate at a boundary portion between the measuring tank 14c and the liquid flow path 12 on the upstream side of the measuring tank 14c. Only the base end 18b is fixed to the bottom of the liquid flow path 12 so that the tip 18a of the 18 is inclined to the downstream side, and the tip 18a and both side ends are not fixed.
Therefore, in FIG. 6A, FIG. 6B and FIG. 7, when liquid is sent from the filtration tank (not shown) to the measuring tank 14c, the liquid flows over the tip 18a of the weir plate 18 into the measuring tank 14c. Can do.
On the other hand, when the liquid feeding part P1 of the measuring tank 14c is activated and the internal volume of the measuring tank 14c is reduced, such a weir plate 18 is arranged, as shown in FIG. The liquid in the measuring tank 14c is fed only to the downstream side, and does not flow backward to the upstream side, that is, the filtration tank side.

　なお、混合機能槽１４ｄは、上述したように、その上流側の測定槽１４ｉとの間で測定液を行き来させることで、測定液を十分に攪拌混合するために設けられている。よって、混合機能槽１４ｄには、液体の上流側への逆流を防止する逆流防止部を設ける必要はない。 As described above, the mixing function tank 14d is provided in order to sufficiently stir and mix the measurement liquid by moving the measurement liquid back and forth between the measurement tank 14i on the upstream side. Therefore, it is not necessary to provide the backflow prevention part which prevents the backflow of the liquid to the upstream side in the mixing function tank 14d.

　この液体流路装置１０Ａを用いて、測定液を調製する具体的な方法としては、まず、この液体流路装置１０Ａを試料投入槽１４ａ側が上方に、測定槽１４ｉ側が下方に位置するように立てて、液体が重力によって上流側から下流側に流れやすい状態とする。
　ついで、試料をシリンジなどにサンプリングし、このシリンジの針を試料投入槽１４ａに対応する部分の蓋板１３に突き刺して、試料投入槽１４ａに試料を注入する。その後、試料投入槽１４ａとろ過槽１４ｂとの間に設けられた開通部Ｓ１を上述の押圧操作、すなわち、第１基材層１３ａを表面側から押圧して荷重を加えた後、取り去る操作で作動させ、この部分の液体流路１２を開通状態とし、試料を重力によりろ過槽１４ｂまで導入する。
　この際、押圧操作は、作業者が指で第１基材層１３ａを表面側から押す手動により行ってもよいし、押圧位置がＸＹ座標としてあらかじめプログラムされている押圧装置などを使用して、所定の位置を押すようにしてもよい。 As a specific method for preparing the measurement liquid using the liquid channel device 10A, first, the liquid channel device 10A is set up so that the sample introduction tank 14a side is positioned upward and the measurement tank 14i side is positioned downward. Thus, the liquid is likely to flow from the upstream side to the downstream side by gravity.
Next, the sample is sampled into a syringe or the like, and the needle of this syringe is pierced into the cover plate 13 corresponding to the sample loading tank 14a to inject the sample into the sample loading tank 14a. Thereafter, the opening portion S1 provided between the sample charging tank 14a and the filtration tank 14b is pressed as described above, that is, an operation of pressing the first base material layer 13a from the surface side to apply a load and then removing it. The liquid channel 12 in this portion is opened, and the sample is introduced to the filtration tank 14b by gravity.
At this time, the pressing operation may be performed manually by the operator manually pressing the first base material layer 13a from the surface side with a finger, or using a pressing device in which the pressing position is pre-programmed as XY coordinates, You may make it push a predetermined position.

　ついで、ろ過槽１４ｂでろ過処理がなされた後、ろ過槽１４ｂと計量槽１４ｃとの間に設けられた開通部Ｓ２を押圧操作で作動させて、この部分の液体流路１２を開通状態とし、試料を重力により計量槽１４ｃに導入する。
　ついで、計量槽１４ｃにおいて、所定量の試料が溜まって計量された時点で、ろ過槽１４ｂと計量槽１４ｃとの間に設けられた閉止部Ｔ１を押圧操作で作動させて、この部分の液体流路１２を閉止状態とする。このようにして、計量槽１４ｃに上流側からの液体がさらに流入するのを停止させてから、計量槽１４ｃの下流に設けられた開通部Ｓ３を押圧操作で作動させて、この部分の液体流路１２を開通状態とする。ついで、計量槽１４ｃを閉塞する部分の蓋板１３を外側から押圧して、送液部Ｐ１を作動させ、重力の作用と送液部Ｐ１の作用とにより、計量後の試料を第１混合槽１４ｆに導入する。 Next, after the filtration treatment is performed in the filtration tank 14b, the opening portion S2 provided between the filtration tank 14b and the measuring tank 14c is operated by pressing operation, and the liquid flow path 12 of this portion is opened, The sample is introduced into the weighing tank 14c by gravity.
Next, when a predetermined amount of sample is accumulated and weighed in the measuring tank 14c, the closing portion T1 provided between the filtration tank 14b and the measuring tank 14c is operated by a pressing operation, and the liquid flow in this portion is measured. The path 12 is closed. In this way, after further stopping the liquid from the upstream side from flowing into the measuring tank 14c, the opening portion S3 provided downstream of the measuring tank 14c is operated by a pressing operation, and the liquid flow in this portion The path 12 is opened. Subsequently, the lid plate 13 that closes the measuring tank 14c is pressed from the outside to operate the liquid feeding part P1, and the sample after weighing is fed into the first mixing tank by the action of gravity and the action of the liquid feeding part P1. 14f.

　こうして計量後の試料を第１混合槽１４ｆに導入する一方で、第１試薬槽１４ｅと第１混合槽１４ｆとの間の開通部Ｓ４を押圧操作で作動させて第１試薬を第１混合槽１４ｆに導入し、試料と第１試薬とを第１混合槽１４ｆにおいて混合し、中間調製液を調製する。
　ついで、第１混合槽１４ｆと第２混合槽１４ｈとの間の開通部Ｓ５を押圧操作で作動させて、この部分の液体流路１２を開通状態とする。ついで、送液部Ｐ２を送液部Ｐ１と同様にして作動させ、重力の作用と送液部Ｐ２との作用により、第１混合槽１４ｆで調製された中間調製液を第２混合槽１４ｈに導入する。一方、第２試薬槽１４ｇと第２混合槽１４ｈとの間の開通部Ｓ６を押圧操作で作動させて第２試薬を第２混合槽１４ｈに導入し、中間調製液と第２試薬とを第２混合槽１４ｈにおいて混合し、測定液を調製する。 In this way, while the sample after weighing is introduced into the first mixing tank 14f, the opening portion S4 between the first reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, and the first reagent is moved into the first mixing tank 14f. The sample is introduced into 14f, and the sample and the first reagent are mixed in the first mixing tank 14f to prepare an intermediate preparation solution.
Next, the opening part S5 between the first mixing tank 14f and the second mixing tank 14h is operated by a pressing operation, and the liquid flow path 12 in this part is opened. Next, the liquid feeding part P2 is operated in the same manner as the liquid feeding part P1, and the intermediate preparation liquid prepared in the first mixing tank 14f is moved to the second mixing tank 14h by the action of gravity and the action of the liquid feeding part P2. Introduce. On the other hand, the opening part S6 between the second reagent tank 14g and the second mixing tank 14h is operated by a pressing operation to introduce the second reagent into the second mixing tank 14h, and the intermediate preparation solution and the second reagent are supplied to the second reagent tank 14g. 2 Mix in the mixing tank 14h to prepare a measurement solution.

　ついで、第２混合槽１４ｈと測定槽１４ｉとの間の開通部Ｓ７を押圧操作で作動させて、この部分の液体流路１２を開通状態とし、ついで、送液部Ｐ３を作動させて、重力の作用と送液部Ｐ３との作用により、第２混合槽１４ｈで調製された測定液を測定槽１４ｉに導入する。
　ついで、送液部Ｐ４を作動させて、測定槽１４ｉ内の測定液を混合機能槽１４ｄに一旦送液する。その後、送液部Ｐ５を作動させて、混合機能槽１４ｄ内の測定液を測定槽１４ｉに返送する。このような混合操作を必要に応じて繰り返して、測定液を十分に攪拌混合した後、この液体流路装置１０Ａごと検出分析部に供し、測定槽１４ｉ内の測定液について、目的成分の検出や測定を行う。
　なお、このように送液部Ｐ１～Ｐ５を作動させ、液体流路装置１０Ａの液体流路１２に液体を流通させる場合には、必要に応じて、各液槽に設けられている図示略の連通孔を適宜開閉して、液体がより円滑に流れるようにすることが好適である。例えば、送液部Ｐ１を作動させるにあたって、計量槽１４ｃに対応する部分の蓋板１３を押圧した後、押圧を解除する前に計量槽１４ｃに設けられた連通孔を閉止状態から開通状態とし、その後、押圧を解除することにより、計量槽１４ｃ内が減圧状態となって下流に送液された液体が計量槽１４ｃに逆流することを防止できる。 Next, the opening part S7 between the second mixing tank 14h and the measuring tank 14i is operated by pressing, so that the liquid flow path 12 in this part is opened, and then the liquid feeding part P3 is operated, and gravity is applied. The measuring liquid prepared in the second mixing tank 14h is introduced into the measuring tank 14i by the action of and the action of the liquid feeding part P3.
Next, the liquid feeding unit P4 is operated to temporarily feed the measurement liquid in the measurement tank 14i to the mixing function tank 14d. Thereafter, the liquid feeding part P5 is operated to return the measurement liquid in the mixing function tank 14d to the measurement tank 14i. Such a mixing operation is repeated as necessary, and the measurement liquid is sufficiently stirred and mixed. Then, the entire liquid flow path device 10A is supplied to the detection analysis unit, and the target liquid is detected and measured in the measurement tank 14i. Measure.
In addition, when the liquid feeding parts P1 to P5 are operated as described above and the liquid is circulated through the liquid flow path 12 of the liquid flow path device 10A, as shown in FIG. It is preferable that the communication hole is appropriately opened and closed so that the liquid flows more smoothly. For example, in operating the liquid feeding part P1, after pressing the part of the cover plate 13 corresponding to the measuring tank 14c, before releasing the press, the communication hole provided in the measuring tank 14c is changed from the closed state to the open state, Thereafter, by releasing the pressure, it is possible to prevent the liquid in the measuring tank 14c from being reduced in pressure and flowing downstream to flow back into the measuring tank 14c.

　このような液体流路装置１０Ａによれば、液槽内の液体を送液する送液部Ｐ１～Ｐ５が、計量槽１４ｃと、第１混合槽１４ｆと、第２混合槽１４ｈと、測定槽１４ｉと、混合機能槽１４ｄとにそれぞれ設けられているため、たとえ試料、中間調製液、測定液が粘性を有するなどして、液体流路１２を流れ難いものである場合でも、液体を流通させるための装置を別途必要とすることなく、液体を簡便かつ円滑に流通させることができる。
　また、この例の送液部Ｐ１～Ｐ５は、液体流路装置１０Ａの蓋板１３を利用した構成であるため、送液部Ｐ１～Ｐ５のための別部材を新たに用意する必要がなく、低コストであるとともに構成もシンプルである。また、送液部Ｐ１～Ｐ５も押圧だけの簡便な操作で作動するため、操作性にも優れる。 According to such a liquid channel device 10A, the liquid feeding parts P1 to P5 for feeding the liquid in the liquid tank include the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, and the measuring tank. 14i and the mixing function tank 14d are respectively provided, so that the liquid is circulated even if the sample, the intermediate preparation liquid, and the measurement liquid are difficult to flow through the liquid flow path 12 due to their viscosity. Therefore, the liquid can be easily and smoothly circulated without requiring a separate device.
In addition, since the liquid feeding parts P1 to P5 in this example are configured using the lid plate 13 of the liquid flow path device 10A, it is not necessary to prepare another member for the liquid feeding parts P1 to P5. It is low cost and simple in construction. Further, since the liquid feeding parts P1 to P5 are also operated by a simple operation only by pressing, the operability is excellent.

　さらに、この例では、送液部Ｐ１～Ｐ４がそれぞれ設けられた計量槽１４ｃと第１混合槽１４ｆと第２混合槽１４ｈと測定槽１４ｉとには、逆流防止部Ｇ１～Ｇ６としての堰板１８が設けられているため、送液部Ｐ１～Ｐ４を作動させた場合に液体が上流側へと逆流することもない。 Further, in this example, the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14h, and the measuring tank 14i provided with the liquid feeding parts P1 to P4, respectively, are the weir plates as the backflow prevention parts G1 to G6. 18 is provided, the liquid does not flow backward to the upstream side when the liquid feeding parts P1 to P4 are operated.

　さらにこの例の液体流路装置１０Ａは、液体流路１２を閉止状態から開通状態にする開通部Ｓ１～Ｓ７と、開通状態から閉止状態にする閉止部Ｔ１とを有するため、液体流路１２中の液体の流れを制御でき、その結果、精度の高い検出や分析を速やかに行うことができる。
　例えば、この例では、計量槽１４ｃの上流には閉止部Ｔ１が設けられ、下流には開通部Ｓ３が設けられている。そのため、計量槽１４ｃで試料を正確かつ速やかに計量して、第１混合槽１４ｆに導入することができる。ここで仮に、計量槽１４ｃの下流に開通部Ｓ３が設けられておらず、この部分の液体流路１２が常に開通した状態であると、計量中であっても計量槽１４ｃから試料が連続的に流出してしまい、試料を一定量溜めることができず、計量自体が困難となる。また、計量槽１４ｃの上流に閉止部Ｔ１が設けられていない場合には、一定量の試料を計量槽１４ｃに溜めた後にも、試料投入槽１４ａに注入された試料の量によっては、ろ過槽１４ｂを経た試料が計量槽１４ｃに流入し続け、やはり、計量自体が困難となる可能性がある。その点、この例のように、計量槽１４ｃの上流に閉止部Ｔ１が設けられていると、ろ過槽１４ｂを経た試料の全量が計量槽１４ｃに完全に流入し終わらなくても、計量槽１４ｃにおいて一定量の試料が計量された時点で閉止部Ｔ１を作動させて、計量槽１４ｃへの試料のさらなる流入を停止することができ、試料を正確かつ速やかに計量することができる。 Further, the liquid channel device 10A of this example includes the opening portions S1 to S7 that make the liquid channel 12 from the closed state to the open state and the closing portion T1 that makes the liquid channel 12 from the open state to the closed state. As a result, highly accurate detection and analysis can be performed promptly.
For example, in this example, a closing portion T1 is provided upstream of the measuring tank 14c, and an opening portion S3 is provided downstream. Therefore, the sample can be accurately and quickly measured in the measuring tank 14c and introduced into the first mixing tank 14f. Here, if the opening part S3 is not provided downstream of the measuring tank 14c, and the liquid channel 12 in this part is always open, the sample is continuously transferred from the measuring tank 14c even during measurement. The sample cannot be stored in a certain amount, and the measurement itself becomes difficult. Further, in the case where the closing portion T1 is not provided upstream of the measuring tank 14c, a filtration tank may be used depending on the amount of the sample injected into the sample feeding tank 14a even after a certain amount of sample is stored in the measuring tank 14c. The sample that has passed through 14b continues to flow into the weighing tank 14c, and the weighing itself may still be difficult. In this regard, as in this example, when the closing portion T1 is provided upstream of the measuring tank 14c, the entire amount of the sample that has passed through the filtration tank 14b does not end completely flowing into the measuring tank 14c, but the measuring tank 14c. When a certain amount of sample is weighed in, the closing portion T1 can be operated to stop further inflow of the sample into the weighing tank 14c, and the sample can be accurately and quickly measured.

　また、この例では、第１混合槽１４ｆと第２混合槽１４ｈとの間に開通部Ｓ５が設けられ、第２混合槽１４ｈと測定槽１４ｉとの間に開通部Ｓ７が設けられている。そのため、第１混合槽１４ｆおよび第２混合槽１４ｈにおいて、目的の混合や反応が十分に進行してから、これら開通部Ｓ５、Ｓ７を開通させ、ついで、送液部Ｐ２、Ｐ３を作動させ、中間調製液や測定液をそれぞれ第２混合槽１４ｈや測定槽１４ｉに導入することができる。よって、混合や反応が不十分なことに起因する検出や分析の精度低下を防止することができる。 In this example, an opening S5 is provided between the first mixing tank 14f and the second mixing tank 14h, and an opening S7 is provided between the second mixing tank 14h and the measurement tank 14i. Therefore, in the first mixing tank 14f and the second mixing tank 14h, after the target mixing and reaction have sufficiently progressed, the opening parts S5 and S7 are opened, and then the liquid feeding parts P2 and P3 are operated. The intermediate preparation liquid and the measurement liquid can be introduced into the second mixing tank 14h and the measurement tank 14i, respectively. Therefore, it is possible to prevent a decrease in detection and analysis accuracy due to insufficient mixing and reaction.

　さらに、この例では、第１試薬槽１４ｅと第１混合槽１４ｆとの間、第２試薬槽１４ｇと第２混合槽１４ｈとの間にも開通部Ｓ４、Ｓ６が設けられているため、所望の時点でこれらを開通させて、あらかじめ第１試薬槽１４ｅおよび第２試薬槽１４ｇにそれぞれ封入されている第１試薬および第２試薬を第１混合槽１４ｆや第２混合槽１４ｈに流入させることができる。仮に開通部Ｓ４、Ｓ６が設けられていない場合には、液体流路装置１０Ａの保管時などに、第１試薬および第２試薬が下流側に流れ始めてしまうおそれがある。 Furthermore, in this example, since the opening portions S4 and S6 are provided between the first reagent tank 14e and the first mixing tank 14f and between the second reagent tank 14g and the second mixing tank 14h, it is desirable. These are opened at the point of time, and the first reagent and the second reagent previously sealed in the first reagent tank 14e and the second reagent tank 14g, respectively, are caused to flow into the first mixing tank 14f and the second mixing tank 14h. Can do. If the opening portions S4 and S6 are not provided, the first reagent and the second reagent may start to flow downstream when the liquid channel device 10A is stored.

　また、この例の液体流路装置１０Ａの開通部Ｓ１～Ｓ７および閉止部Ｔ１は、液体流路１２に形成された第１凸部１５および第２凸部１６と蓋板１３とが組み合わされているため、液体流路１２を開通したり閉止したりするための別部材を新たに用意する必要がなく、低コストであるとともに構成もシンプルである。また、開通および閉止の操作も簡便な押圧操作のみで、操作性にも優れる。 Further, the opening portions S1 to S7 and the closing portion T1 of the liquid channel device 10A in this example are a combination of the first convex portion 15 and the second convex portion 16 formed in the liquid channel 12 and the lid plate 13. Therefore, it is not necessary to prepare another member for opening and closing the liquid flow path 12, and the cost is low and the configuration is simple. In addition, the opening and closing operations can be performed only by a simple pressing operation, and the operability is excellent.

　なお、以上例示した液体流路装置１０Ａでは、計量槽１４ｃと、第１混合槽１４ｆと、第２混合槽１４ｆと、測定槽１４ｉと、混合機能槽１４ｄとが送液部Ｐ１～Ｐ５をそれぞれ有する形態とした。しかしながら、これらの全ての液槽が送液部を有していなくてもよく、また、これら以外の他の液槽が送液部を有していてもよい。すなわち、試料や試薬の種類、特性などに応じて、どの液槽に送液部を備えるか適宜決定することができる。例えば、この例では、液体流路装置１０Ａを用いて測定液を調製する場合には、まず、この液体流路装置１０Ａを試料投入槽１４ａ側が上方に、測定槽１４ｉ側が下方に位置するように立てて、液体が重力によって上流側から下流側に流れやすい状態としたうえで、送液部Ｐ１～Ｐ５をも送液に利用する形態、すなわち、重力の作用と送液部の作用とを併用して、液体を流通させる形態について例示した。しかしながら、すべての液槽に送液部を設けることによって、重力を利用しなくても、液体を送液できるように液体流路装置を構成することもできる。
　また、液体流路装置１０Ａでは、９の液槽が形成された形態としたが、液槽の種類、数、配置の順序などは目的に応じて適宜設定できる。 In the liquid channel device 10A exemplified above, the measuring tank 14c, the first mixing tank 14f, the second mixing tank 14f, the measuring tank 14i, and the mixing function tank 14d respectively supply the liquid feeding parts P1 to P5. It was set as the form which has. However, all these liquid tanks may not have a liquid feeding part, and other liquid tanks other than these may have a liquid feeding part. That is, it is possible to appropriately determine which liquid tank is provided with the liquid feeding unit according to the type and characteristics of the sample and the reagent. For example, in this example, when the measurement liquid is prepared using the liquid flow path device 10A, first, the liquid flow path device 10A is positioned so that the sample introduction tank 14a side is positioned upward and the measurement tank 14i side is positioned downward. Stand up and make it easy for the liquid to flow from the upstream side to the downstream side by gravity, and also use the liquid feeding parts P1 to P5 for liquid feeding, that is, the action of gravity and the action of the liquid feeding part are used in combination Then, an example of the mode of circulating the liquid was illustrated. However, the liquid flow path device can be configured so that the liquid can be fed without using gravity by providing the liquid feeding section in all the liquid tanks.
Further, in the liquid channel device 10A, nine liquid tanks are formed. However, the type, number, order of arrangement, and the like of the liquid tanks can be appropriately set according to the purpose.

　さらに、この例の液体流路装置１０Ａでは、送液部Ｐ１～Ｐ４が設けられた液槽に配置される逆流防止部Ｇ１～Ｇ６として、堰板１８が用いられているが、逆流防止部の形態には制限はなく、堰板１８以外のものであってもよい。また、堰板１８を設ける場合においても、２枚以上の堰板１８を直列に配置するなどして、逆流防止効果がより得られるようにしてもよいし、堰板１８と他の逆流防止部を併用してもよい。 Furthermore, in the liquid channel device 10A of this example, the weir plate 18 is used as the backflow prevention units G1 to G6 disposed in the liquid tanks provided with the liquid feeding units P1 to P4. There is no restriction | limiting in a form, A thing other than the weir board 18 may be sufficient. In the case where the barrier plate 18 is provided, two or more barrier plates 18 may be arranged in series so that the effect of preventing the reverse flow can be further obtained. May be used in combination.

　さらに、逆流防止部Ｇ１～Ｇ６を設ける代わりに、その箇所において、液体流路１２を開通状態から閉止状態にする閉止部を設け、送液部Ｐ１～Ｐ４を作動させる前に閉止部を作動させることにより、液体が上流側へ逆流することを防ぐようにすることも可能である。
　このように閉止部を逆流防止目的に使用する形態は、例えば、計量槽として、計量槽に連通したオーバーフロー流路とその下流に設けられた廃液槽とからなるオーバーフロー部が備えられたものを採用した場合などに特に有効である。このような計量槽は、計量槽で一定量を超えた試料がオーバーフローしてオーバーフロー流路を流れ、廃液槽に流入し、その結果、計量槽で一定量の試料を計量することができる。そのため、計量の際には、計量槽からオーバーフロー流路へスムーズに試料が流れる必要がある。一方、計量槽に備えられた送液部を作動させ、計量後の試料を下流側に送る場合には、試料が計量槽からオーバーフロー流路に流れ込むことを防止する必要がある。このように、両方向に試料が流れる必要がある箇所には、逆流防止部を設けることはできない。
よって、このような箇所には、閉止部を設け、必要な場合にのみ、その箇所を閉止状態にできるように構成することが好ましい。 Further, instead of providing the backflow prevention parts G1 to G6, a closing part for changing the liquid flow path 12 from the open state to the closed state is provided at that point, and the closing part is operated before the liquid feeding parts P1 to P4 are operated. Thus, it is also possible to prevent the liquid from flowing back to the upstream side.
In this way, the form in which the closing part is used for the purpose of preventing the backflow, for example, employs a measuring tank equipped with an overflow part composed of an overflow channel communicating with the measuring tank and a waste liquid tank provided downstream thereof. This is particularly effective when In such a measuring tank, a sample exceeding a certain amount in the measuring tank overflows, flows through the overflow channel, and flows into the waste liquid tank. As a result, a certain amount of sample can be measured in the measuring tank. Therefore, it is necessary for the sample to flow smoothly from the measuring tank to the overflow channel when measuring. On the other hand, when the liquid feeding section provided in the measuring tank is operated to send the sample after measurement to the downstream side, it is necessary to prevent the sample from flowing into the overflow channel from the measuring tank. Thus, a backflow prevention unit cannot be provided at a location where the sample needs to flow in both directions.
Therefore, it is preferable to provide a closing portion at such a location so that the location can be closed only when necessary.

　図８は　本発明の液体流路装置の第２実施形態を示すものであって、この液体流路装置１０Ｂは、扇状の平板からなる基板１１Ｂの片面に、先に説明した液体流路装置１０Ａと同様に、試料および試薬の少なくとも一方からなる液体が流通する溝状の液体流路１２と、液体流路１２の端部や途中において液体が溜まる複数の液槽（１４ａ、１４ｅ～１４ｈ、１４Ｊ、１４ｋ、１４ｍ）とが形成され、基板１１の液体流路１２が形成された側の流路形成面１２ａに蓋板１３が積層して構成されたものである。この液体流路装置１０Ｂにおいては、図８中の上端部側を中心に回転させた場合には、液体流路１２の上流側の端部から下流側の端部に向けて矢印Ｆ’方向に試料が遠心力により流通し、その途中で試料に対して各種の処理や試薬との混合がなされ、測定液とされる。 FIG. 8 shows a second embodiment of the liquid flow path device of the present invention. This liquid flow path device 10B is a liquid flow path device 10A described above on one side of a substrate 11B made of a fan-shaped flat plate. In the same manner as described above, a groove-like liquid channel 12 through which a liquid composed of at least one of a sample and a reagent flows, and a plurality of liquid tanks (14a, 14e to 14h, 14J) in which the liquid is accumulated at the end or in the middle of the liquid channel 14k, 14m), and the cover plate 13 is laminated on the flow path forming surface 12a of the substrate 11 on the side where the liquid flow path 12 is formed. In the liquid channel device 10B, when rotated about the upper end side in FIG. 8, in the direction of arrow F ′ from the upstream end of the liquid channel 12 toward the downstream end. The sample is circulated by centrifugal force, and the sample is mixed with various treatments and reagents in the middle of the sample to obtain a measurement solution.

　すなわち、液体流路１２の上流側の端部には、投入された試料が溜まる試料投入槽１４ａが設けられ、この試料投入槽１４ａの下流には、第１試薬槽１４ｅからの第１試薬および第２試薬槽１４ｇからの第２試薬と試料投入槽１４ａからの試料とが混合され、中間調製液が調製される第１混合槽１４ｆが設けられている。
　第１混合槽１４ｆの下流には、第３試薬槽１４ｊからの第３試薬および第４試薬槽１４ｋからの第４試薬と第１混合槽１４ｆからの中間調製液とが混合される第２混合槽１４ｈが設けられている。
　この例では、第２混合槽１４ｈは測定槽としても作用し、第２混合槽１４ｈで調製された測定液に対して、図示略の検出分析部により、各種成分の検出や分析がなされる。
　また、この例では、第２混合槽１４ｈで測定された後の測定液を貯留しておく廃棄槽１４ｍが形成されている。
なお、各液槽には、大気と連通する図示略の連通孔が設けられている。 That is, the upstream end portion of the liquid flow path 12 is provided with a sample introduction tank 14a in which the introduced sample is stored, and the first reagent and the first reagent from the first reagent tank 14e are provided downstream of the sample introduction tank 14a. There is provided a first mixing tank 14f in which the second reagent from the second reagent tank 14g and the sample from the sample charging tank 14a are mixed to prepare an intermediate preparation solution.
Downstream of the first mixing tank 14f is a second mixing in which the third reagent from the third reagent tank 14j, the fourth reagent from the fourth reagent tank 14k, and the intermediate preparation liquid from the first mixing tank 14f are mixed. A tank 14h is provided.
In this example, the second mixing tank 14h also functions as a measurement tank, and various components are detected and analyzed by a detection analysis unit (not shown) with respect to the measurement liquid prepared in the second mixing tank 14h.
In this example, a disposal tank 14m is formed for storing the measurement liquid after being measured in the second mixing tank 14h.
Each liquid tank is provided with a communication hole (not shown) that communicates with the atmosphere.

　また、この液体流路装置１０Ｂにおいても、蓋板１３は上述の液体流路装置１０Ａの場合と同様の図３に示す構成、すなわち、蓋板１３の表面を構成する第１基材層１３ａと、第１基材層１３ａの内側に形成された強粘着層１３ｂと、強粘着層１３ｂの内側に形成された第２基材層１３ｃと、第２基材層１３ｃの内側に形成され、流路形成面に粘着する弱粘着層１３ｄとを有している。 Also in this liquid channel device 10B, the lid plate 13 has the same configuration as shown in FIG. 3 as in the case of the above-described liquid channel device 10A, that is, the first base material layer 13a constituting the surface of the lid plate 13 and A strong adhesive layer 13b formed inside the first base material layer 13a, a second base material layer 13c formed inside the strong adhesive layer 13b, and formed inside the second base material layer 13c. It has a weak adhesive layer 13d that adheres to the path forming surface.

　また、試料投入槽１４ａと第１混合槽１４ｆとの間、第１試料槽１４ｅと第１混合層１４ｆとの間、第２試料槽１４ｇと第１混合槽１４ｆとの間、第１混合槽１４ｆと第２混合槽１４ｈとの間、第３試料槽１４ｊと第２混合層１４ｈとの間、第４試料槽１４ｋと第２混合槽１４ｈとの間、第２混合槽１４ｈと廃棄槽１４ｍとの間には、液体流路１２を閉止状態から開通状態とする開通部Ｓ８～Ｓ１４が１つずつ設けられている。
　一方、試料投入槽１４ａと第１混合槽１４ｆとの間の液体流路１２において、開通部Ｓ８よりも下流側に、閉止部Ｔ２が設けられている。 Also, between the sample loading tank 14a and the first mixing tank 14f, between the first sample tank 14e and the first mixing layer 14f, between the second sample tank 14g and the first mixing tank 14f, the first mixing tank. 14f and the second mixing tank 14h, the third sample tank 14j and the second mixing layer 14h, the fourth sample tank 14k and the second mixing tank 14h, the second mixing tank 14h and the disposal tank 14m. Are provided with one opening portion S8 to S14 for bringing the liquid flow path 12 from the closed state to the open state.
On the other hand, in the liquid flow path 12 between the sample introduction tank 14a and the first mixing tank 14f, a closing part T2 is provided on the downstream side of the opening part S8.

　そして、液体流路装置１０Ａの場合と同様に、各開通部Ｓ８～Ｓ１４では、図３に示したように、液体流路１２に第１凸部１５が形成され、この第１凸部１５の頂部１５ａと弱粘着層１３ｄとが粘着し、かつ、強粘着層１３ｂと第２基材層１３ｃとが離間している。一方、閉止部Ｔ２では、液体流路１２に第２凸部１６が形成され、この第２凸部１６の頂部１６ａと弱粘着層１３ｄとは離間し、かつ、強粘着層１３ｂと第２基材層１３ｃとの間にはスペーサ部材１７が介在し、スペーサ部材１７と強粘着層１３ｂとが粘着している。 As in the case of the liquid channel device 10A, in each of the opening portions S8 to S14, as shown in FIG. 3, the first convex portion 15 is formed in the liquid channel 12, and the first convex portion 15 The top portion 15a and the weak adhesive layer 13d are adhered to each other, and the strong adhesive layer 13b and the second base material layer 13c are separated from each other. On the other hand, in the closing portion T2, the second convex portion 16 is formed in the liquid flow path 12, the top portion 16a of the second convex portion 16 and the weak adhesive layer 13d are separated from each other, and the strong adhesive layer 13b and the second base are formed. A spacer member 17 is interposed between the material layer 13c and the spacer member 17 and the strong adhesion layer 13b are adhered.

　この液体流路装置１０Ｂを用いて、測定液を調製する場合には、まず、この液体流路装置１０Ｂを試料投入槽１４ａ側が回転中心側に位置し、測定槽１４ｉ側が回転の外周側に位置するように遠心装置にセットする。
　ついで、試料をシリンジなどにサンプリングし、このシリンジの針を試料投入槽１４ａに対応する部分の蓋板１３に突き刺して、試料投入槽１４ａに試料を注入する。その後、遠心装置を作動させることによって、回転中心側から外周側に作用する遠心力が生じ、この遠心力により、液体が上流側から下流側に流れ始める。
　ついで、試料投入槽１４ａと第１混合槽１４ｆとの間に設けられた開通部Ｓ８を液体流路装置１０Ａの場合と同様の押圧操作で作動させ、この部分の液体流路１２を開通状態とし、試料を遠心力により第１混合槽１４ｆに導入する。 When preparing a measurement liquid using the liquid channel device 10B, first, the liquid channel device 10B is positioned on the rotation center side on the sample loading tank 14a side, and the measurement tank 14i side is positioned on the outer peripheral side of the rotation. Set to centrifuge.
Next, the sample is sampled into a syringe or the like, and the needle of this syringe is pierced into the cover plate 13 corresponding to the sample loading tank 14a to inject the sample into the sample loading tank 14a. Thereafter, by operating the centrifugal device, a centrifugal force acting from the rotation center side to the outer peripheral side is generated, and by this centrifugal force, the liquid starts to flow from the upstream side to the downstream side.
Next, the opening part S8 provided between the sample charging tank 14a and the first mixing tank 14f is operated by the same pressing operation as in the case of the liquid channel device 10A, so that the liquid channel 12 in this part is opened. The sample is introduced into the first mixing tank 14f by centrifugal force.

　こうして遠心力を生じさせながら、試料を第１混合槽１４ｆに導入する一方で、第１試薬槽１４ｅと第１混合槽１４ｆとの間の開通部Ｓ９を押圧操作で作動させて、あらかじめ封入されていた第１試薬を第１混合槽１４ｆに導入し、ついで、第２試薬槽１４ｅと第１混合槽１４ｆとの間の開通部Ｓ１０を押圧操作で作動させて、あらかじめ封入されていた第２試薬も第１混合槽１４ｆに導入し、試料と第１試薬と第２試薬とを第１混合槽１４ｆにおいて混合する。
　そして、この際に、必要に応じて、試料の全量が完全に第１混合槽１４ｆに流入し終わる前に、閉止部Ｔ２を作動させることによって、試料の第１混合槽１４への過剰な流入を止めることもできる。 While the centrifugal force is generated in this way, the sample is introduced into the first mixing tank 14f, while the opening portion S9 between the first reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, and is enclosed in advance. The first reagent that had been introduced is introduced into the first mixing tank 14f, and then the opening portion S10 between the second reagent tank 14e and the first mixing tank 14f is operated by a pressing operation, so that the second sealed in advance The reagent is also introduced into the first mixing tank 14f, and the sample, the first reagent, and the second reagent are mixed in the first mixing tank 14f.
At this time, if necessary, before the entire amount of the sample completely flows into the first mixing tank 14f, the inflow of the sample into the first mixing tank 14 is activated by operating the closing portion T2. Can also be stopped.

　ついで、第１混合槽１４ｆと第２混合槽１４ｈとの間の開通部Ｓ１１を押圧操作で作動させて、第１混合槽１４ｆで調製された中間調製液を第２混合槽１４ｈに導入する一方で、第３試薬槽１４ｇと第２混合槽１４ｈとの間の開通部Ｓ１２と、第３試薬槽１４ｇと第２混合槽１４ｈとの間の開通部Ｓ１３とを押圧操作で作動させて、あらかじめ封入されていた第３試薬および第４試薬も第２混合槽１４ｈに導入し、中間調製液と第３試薬と第４試薬とを第２混合槽１４ｈにおいて混合する。
　そして、この液体流路装置１０Ｂごと検出分析部に供し、第２混合槽で調製された測定液に対して、目的成分の検出や分析を行う。
　検出や分析が終了した後には、開通部Ｓ１４を作動させて、測定後の測定液を廃棄槽１４ｍへ廃棄することもできる。 Next, the opening S11 between the first mixing tank 14f and the second mixing tank 14h is operated by a pressing operation to introduce the intermediate preparation liquid prepared in the first mixing tank 14f into the second mixing tank 14h. Then, the opening part S12 between the third reagent tank 14g and the second mixing tank 14h and the opening part S13 between the third reagent tank 14g and the second mixing tank 14h are actuated by pressing operation in advance. The sealed third reagent and fourth reagent are also introduced into the second mixing tank 14h, and the intermediate preparation liquid, the third reagent, and the fourth reagent are mixed in the second mixing tank 14h.
Then, the entire liquid flow path device 10B is supplied to the detection analysis unit, and the target component is detected and analyzed for the measurement liquid prepared in the second mixing tank.
After the detection and analysis are completed, the opening portion S14 can be operated to discard the measurement liquid after measurement into the waste tank 14m.

　このような液体流通装置１０Ｂによっても、液体流路１２を閉止状態から開通状態にする開通部Ｓ８～Ｓ１４と、開通状態から閉止状態にする閉止部Ｔ２とを有するため、液体流路１２中の液体の流れを制御でき、その結果、検出や分析を良好な精度で短時間に行うことができる。
　また、開通部Ｓ８～Ｓ１４および閉止部Ｔ２も、低コストであるとともに構成もシンプルで、しかも、簡便な押圧操作のみで操作することができる。 Such a liquid circulation device 10B also includes the opening portions S8 to S14 that make the liquid flow channel 12 from the closed state to the open state and the closing portion T2 that makes the liquid flow channel 12 from the open state to the closed state. The flow of the liquid can be controlled, and as a result, detection and analysis can be performed with good accuracy in a short time.
Further, the opening portions S8 to S14 and the closing portion T2 are low in cost and simple in configuration, and can be operated only by a simple pressing operation.

　なお、この例の液体流路装置１０Ｂの場合にも、開通部Ｓ８～Ｓ１４および閉止部Ｔ２における押圧操作を手動で行えるが、液体流路装置１０Ｂの蓋板１３の表面を圧接する圧接ディスクにより押圧すると、複数枚の液体流路装置１０Ｂに対して、連続的に押圧操作ができ好ましい。
　図９は、遠心装置の有する基盤２０上に円形になるように並べられてセットされた複数枚（この例では６枚）の扇状（中心角α＝６０°）の液体流路装置１０Ｂに対して、圧接ディスク２１により押圧操作する方法を示したものである。この例の圧接ディスク２１は、液体流路装置１０Ｂを回転させる遠心装置の回転軸２２から側方に延びたアーム２３に軸設され、アーム２３を中心として回転しながら、アーム２３の長さ方向（液体流路装置の回転の半径方向）に沿って、液体流路装置１０Ｂの回転中心側から外周側に向けて移動する。よって、遠心装置により液体流路装置１０Ｂを回転させながら、圧接ディスク２１をこのように移動させることによって、圧接ディスク２１は円形に並べられた液体流路装置１０Ｂ上を回転中心側から外周側に向けて相対的に渦巻き状に走査し、これら液体流路装置１０Ｂに設けられた開通部Ｓ８～１４および閉止部Ｔ２を順次押圧操作することができる。 In the case of the liquid channel device 10B of this example as well, the pressing operation at the opening portions S8 to S14 and the closing portion T2 can be manually performed. When pressed, a plurality of liquid flow path devices 10B can be continuously pressed, which is preferable.
FIG. 9 illustrates a plurality of (six in this example) fan-shaped (center angle α = 60 °) liquid channel device 10B set in a circular pattern on a base plate 20 of the centrifugal device. The method of pressing with the press contact disk 21 is shown. The pressure contact disk 21 in this example is provided on an arm 23 extending laterally from a rotation shaft 22 of a centrifugal device that rotates the liquid flow path device 10B, and rotates in the length direction of the arm 23 while rotating around the arm 23. Along the (radial direction of rotation of the liquid flow path device), the liquid flow path device 10B moves from the rotation center side toward the outer peripheral side. Therefore, by moving the pressure contact disk 21 in this way while rotating the liquid flow path device 10B by the centrifugal device, the pressure contact disk 21 moves on the liquid flow path device 10B arranged in a circle from the rotation center side to the outer peripheral side. It is possible to scan in a relatively spiral shape and sequentially press the opening portions S8 to S14 and the closing portion T2 provided in the liquid channel device 10B.

　なお、以上の説明では、液体流路装置１０Ａ、１０Ｂの試料投入槽１４ａに試料を注入する方法として、シリンジの針を蓋板１３に突き刺す方法を例示しているが、例えば、あらかじめ蓋板１３に試料注入孔を形成しておき、そこから試料を注入してもよい。その場合、試料注入孔には保護テープを被せておき、シリンジを保護テープに突き刺すことで注入してもよいし、保護テープを剥がして試料注入孔にシリンジを挿入して注入してもよい。 In the above description, as a method of injecting a sample into the sample introduction tank 14a of the liquid flow path apparatuses 10A and 10B, a method of piercing the needle of the syringe into the cover plate 13 is exemplified. Alternatively, a sample injection hole may be formed, and the sample may be injected therefrom. In that case, the sample injection hole may be covered with a protective tape and injected by piercing the syringe with the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.

　以上説明した液体流路装置１０Ａ、１０Ｂにおいて、液体流路１２および液槽が形成される基板１１Ａ、１１Ｂには、例えば、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂、エポキシ樹脂、フェノール樹脂、ＡＢＳ樹脂、ポリプロピレン樹脂、繊維強化プラスチックなどの樹脂板や、ガラス板が使用できる。これらのなかでも、透明であって、液体流路１２を流通する液体の様子を基板１１Ａ、１１Ｂ側から目視することができる点では、ガラス板や、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂が好ましい。また、ガラス板よりも破損しにくく取扱性に優れる点では、樹脂板の方が好ましい。
　基板１１Ａ、１１Ｂの厚さには特に制限はなく、形成される液体流路１２の深さなどに応じて決定されればよいが、通常０．５～７ｍｍである。 In the liquid flow path apparatuses 10A and 10B described above, the substrates 11A and 11B on which the liquid flow path 12 and the liquid tank are formed include, for example, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin. A resin plate such as an epoxy resin, a phenol resin, an ABS resin, a polypropylene resin, or a fiber reinforced plastic, or a glass plate can be used. Among these, a glass plate, a styrene resin, an acrylic resin, a polycarbonate resin, and vinyl chloride are transparent in that the state of the liquid flowing through the liquid flow path 12 can be visually observed from the side of the substrates 11A and 11B. Resin, PEN resin and polyester resin are preferred. In addition, a resin plate is preferable in that it is less likely to break than a glass plate and has excellent handleability.
The thickness of the substrates 11A and 11B is not particularly limited and may be determined according to the depth of the liquid flow path 12 to be formed, but is usually 0.5 to 7 mm.

　液体流路１２や液槽は、基板１１Ａ、１１Ｂの片面上に、例えばフォトリソグラフィ、射出成形、ブロー成形、接合形成、溶解形成、切削形成、機械加工などの技術により溝状に形成される。
　液体流路１２の断面形状（流れに対して垂直方向の断面）には特に制限はなく、例えば、半円形状、四角形状、逆三角形状などが挙げられる。液体流路１２の幅や深さにも特に制限はなく、求められる液体の流量などに応じて決定されればよいが、幅および深さがそれぞれ１０～５０００μｍの範囲であれば、小さな流路抵抗で液体を流すことができ、かつ、流通させる液体の量も少量ですむ点で好ましい。
　また、液体流路１２には、液体を流れやすくするために、液体の種類に応じた表面処理を施すことが好ましい。このような表面処理としては、塗料の塗布処理、プラズマ処理、フレーム処置、薬品処理、生理活性処理、抗体処理などが挙げられる。さらに、液体流路１２には、必要に応じて、邪魔板、攪拌板、突起を設けたり、分水形状を形成したりして、流通する液体が均一な混合状態となるようにしてもよい。
　各液槽も、形状などには特に制限はなく、各液槽に要求される容積などに応じて適宜形成されればよい。
　各堰板は、可撓性を有する樹脂シートなどで形成され、所定の部位に配されてもよいし、液体流路１２や液槽を形成する際に基板１１Ａから一体に形成されてもよい。 The liquid flow path 12 and the liquid tank are formed in a groove shape on one surface of the substrates 11A and 11B by techniques such as photolithography, injection molding, blow molding, bonding formation, dissolution formation, cutting formation, and machining.
There is no particular limitation on the cross-sectional shape of the liquid flow path 12 (the cross-section in the direction perpendicular to the flow), and examples thereof include a semicircular shape, a quadrangular shape, and an inverted triangular shape. The width and depth of the liquid flow path 12 are not particularly limited, and may be determined according to the required liquid flow rate. However, if the width and depth are in the range of 10 to 5000 μm, the small flow path It is preferable in that a liquid can be flowed by resistance and a small amount of liquid can be circulated.
The liquid flow path 12 is preferably subjected to a surface treatment according to the type of liquid in order to facilitate the flow of the liquid. Examples of such surface treatment include coating application treatment, plasma treatment, flame treatment, chemical treatment, physiological activity treatment, and antibody treatment. Furthermore, the liquid flow path 12 may be provided with a baffle plate, a stirring plate, a protrusion, or formed with a water diverting shape, if necessary, so that the flowing liquid is in a uniform mixed state. .
Each liquid tank is not particularly limited in shape, and may be appropriately formed according to the volume required for each liquid tank.
Each dam plate is formed of a flexible resin sheet or the like, and may be disposed at a predetermined site, or may be integrally formed from the substrate 11A when forming the liquid channel 12 or the liquid tank. .

　蓋板１３の表面を構成する第１基材層１３ａは、その表面側から垂直方向の荷重を加えられた場合には撓み、その後、戻ろうとする復元力を有する。このような特性、すなわち、可撓性と復元力とを有する基材であれば、第１基材層１３ａとして使用でき、その材質や厚みには特に制限はないが、ポリエチレンテレフタレート（ＰＥＴ）、ポリエチレンナフタレート（ＰＥＮ）、ポリカーボネート（ＰＣ）、ポリイミドなどからなる厚さ５０～５００μｍのフィルムであれば、第１基材層１３ａとして使用するのに適切な可撓性と復元力を有するため好ましい。 The first base material layer 13a constituting the surface of the cover plate 13 has a restoring force to bend and then return when a vertical load is applied from the surface side. If it is a base material having such characteristics, that is, flexibility and restoring force, it can be used as the first base material layer 13a, and its material and thickness are not particularly limited, but polyethylene terephthalate (PET), A film having a thickness of 50 to 500 μm made of polyethylene naphthalate (PEN), polycarbonate (PC), polyimide, or the like is preferable because it has flexibility and resilience appropriate for use as the first base material layer 13a. .

　一方、第２基材層１３ｃは、垂直方向の荷重により容易に撓むものであればよく、復元しないものがさらに好ましい。このような特性を有する基材であれば、第２基材層１３ｃとして使用でき、その材質や厚みには特に制限はないが、アルミニウム箔、銅箔などの金属箔、紙、ＰＥＴ、ＰＥＮ、ＰＣ、ポリイミドなどの樹脂からなる厚さ５～５０μｍのフィルムであれば、第２基材層１３ｃとして使用するのに好ましい。紙を使用する場合には、防水処理された紙が好ましく、金属箔を使用する場合には、防錆処理された金属箔が好ましい。 On the other hand, the second base material layer 13c only needs to be easily bent by a load in the vertical direction, and more preferably does not recover. If it is the base material which has such a characteristic, it can be used as the 2nd base material layer 13c, Although there is no restriction | limiting in particular in the material and thickness, Metal foil, such as aluminum foil and copper foil, paper, PET, PEN, A film having a thickness of 5 to 50 μm made of a resin such as PC or polyimide is preferable for use as the second base material layer 13c. When using paper, waterproof paper is preferable, and when using metal foil, rust-proof metal foil is preferable.

　強粘着層１３ｂおよび弱粘着層１３ｄには、従来公知の粘着剤の中から第１基材層１３ａや第２基材層１３ｃの材質などに応じて適宜選択することができるが、その際、強粘着層１３ｂを形成する粘着剤の粘着力（粘着強度）は、弱粘着層１３ｄを形成する粘着剤の粘着力よりも強いことが必要である。強粘着層１３ｂを形成する粘着剤の粘着力が弱粘着層１３ｄを形成する粘着剤の粘着力以下であると、開通部Ｓ１～Ｓ１４において押圧操作をした場合でも、第１凸部１５の頂部１５ａと弱粘着層１３ｄとを離間させることができず、液体流路１２を開通できなくなる。強粘着層１３ｂを形成する粘着剤の粘着力は、弱粘着層１３ｄを形成する粘着剤の粘着力よりも０．１Ｎ／ｃｍ以上大きいことが好ましい。さらには、０．１～３０Ｎ／ｃｍの範囲で大きいことが好ましい。強粘着層１３ｂを形成する粘着剤の粘着力が弱粘着層１３ｄを形成する粘着剤の粘着力よりも０．１Ｎ／ｃｍ以上大きいと、開通部Ｓ１～Ｓ１４を確実に作動させることができる。一方、粘着力の差が３０Ｎ／ｃｍを超えるようにこれらの粘着層を構成することは困難である。
　また、そのうえで、強粘着層１３ｂの粘着力を１～３０Ｎ／ｃｍの範囲とし、弱粘着層１３ｄの粘着力を０．０５～５Ｎ／ｃｍの範囲とすることが好ましい。 The strong adhesive layer 13b and the weak adhesive layer 13d can be appropriately selected from conventionally known adhesives according to the material of the first base material layer 13a and the second base material layer 13c. The adhesive force (adhesive strength) of the adhesive forming the strong adhesive layer 13b needs to be stronger than the adhesive force of the adhesive forming the weak adhesive layer 13d. When the pressure-sensitive adhesive of the pressure-sensitive adhesive forming the strong pressure-sensitive adhesive layer 13b is equal to or lower than the pressure-sensitive adhesive of the pressure-sensitive adhesive forming the weak pressure-sensitive adhesive layer 13d, even when a pressing operation is performed in the opening portions S1 to S14, 15a and the weak adhesion layer 13d cannot be separated, and the liquid channel 12 cannot be opened. It is preferable that the adhesive force of the adhesive forming the strong adhesive layer 13b is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layer 13d. Further, it is preferably large in the range of 0.1 to 30 N / cm. When the adhesive force of the adhesive forming the strong adhesive layer 13b is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layer 13d, the opening portions S1 to S14 can be operated reliably. On the other hand, it is difficult to configure these adhesive layers so that the difference in adhesive strength exceeds 30 N / cm.
In addition, the adhesive strength of the strong adhesive layer 13b is preferably in the range of 1 to 30 N / cm, and the adhesive strength of the weak adhesive layer 13d is preferably in the range of 0.05 to 5 N / cm.

　強粘着層１３ｂおよび弱粘着層１３ｄに使用する粘着剤としては、例えば、アクリル系、ゴム系、ポリウレタン系、ポリエステル系、シリコン系などが挙げられる。これらのうち、例えば、強粘着層１３ｂにはアクリル系、ゴム系などを使用し、さらに芯材として、不織布、ポリエステル繊維などを含ませてもよい。弱粘着層１３ｄには、アクリル系、シリコン系のものを使用することが好ましい。強粘着層１３ｂと弱粘着層１３ｄとの粘着力の差を上述の好適な範囲とするためには、各粘着剤を構成する樹脂のガラス転移温度を適宜調整したり、粘着剤に粘着付与剤、硬化剤、芯材などの添加剤を加えたり、その添加量を調整したりする方法が挙げられる。
　また、強粘着層および弱粘着層の厚さには制限はないが、通常、１０～１０００μｍである。 Examples of the adhesive used for the strong adhesive layer 13b and the weak adhesive layer 13d include acrylic, rubber-based, polyurethane-based, polyester-based, and silicon-based adhesives. Among these, for example, the strong adhesion layer 13b may be made of acrylic or rubber, and may further include a nonwoven fabric, polyester fiber, or the like as a core material. The weak adhesive layer 13d is preferably made of acrylic or silicon. In order to make the difference in adhesive strength between the strong adhesive layer 13b and the weak adhesive layer 13d within the above-mentioned preferable range, the glass transition temperature of the resin constituting each adhesive is appropriately adjusted, or the tackifier is added to the adhesive. And a method of adding an additive such as a curing agent and a core material and adjusting the amount of the additive.
The thickness of the strong adhesive layer and the weak adhesive layer is not limited, but is usually 10 to 1000 μm.

　なお、ここで「粘着力」とは、ＪＩＳ　Ｚ　０２３７のステンレス板に対する１８０度引きはがし粘着力のことである。 In addition, "adhesive strength" here means the adhesive strength by peeling 180 degrees against a stainless steel plate of JIS Z 0237.

　スペーサ部材１７としては、ＰＥＴ、ＰＥＮ、ＰＣ、アクリル樹脂、エポキシ樹脂、フェノール樹脂、ポリウレタン樹脂などの樹脂の他、紙なども使用できる。スペーサ部材１７の厚みには特に制限はないが、５０～２０００μｍの範囲にすると、開通部Ｓ１～Ｓ１４の作動前には、強粘着層１３ｂと第２基材層１３ｃとを確実に離間させておくことができ、一方、作動時には、強粘着層１３ｂと第２基材層１３ｃとを確実に粘着させることができる。 As the spacer member 17, paper such as PET, PEN, PC, acrylic resin, epoxy resin, phenol resin, polyurethane resin, paper, or the like can be used. The thickness of the spacer member 17 is not particularly limited, but if it is in the range of 50 to 2000 μm, the strong adhesion layer 13b and the second base material layer 13c are reliably separated before the opening portions S1 to S14 are operated. On the other hand, at the time of operation, the strong adhesion layer 13b and the second base material layer 13c can be reliably adhered.

［第３実施形態］
　以上説明した第１実施形態の液体流路装置１０Ａでは、送液部Ｐ１～Ｐ５として、液槽に対応する部分の蓋板１３を外側から押圧する操作により、この部分の蓋板１３を撓ませ、その液槽の内容積を小さくすることによって、液体を送液する形態について例示した。第３実施形態では、蓋板１３を外側から押圧する操作の代わりに、液槽の底部を外側から押圧する操作により、液槽の内容積を小さくして、液槽内の液体を送液する形態の送液部について説明する。 [Third Embodiment]
In the liquid channel device 10A of the first embodiment described above, the lid plate 13 of this part is bent by the operation of pressing the lid plate 13 of the part corresponding to the liquid tank from the outside as the liquid feeding parts P1 to P5. An example of a mode in which the liquid is fed by reducing the internal volume of the liquid tank is shown. In the third embodiment, instead of the operation of pressing the lid plate 13 from the outside, the operation of pressing the bottom of the liquid tank from the outside reduces the internal volume of the liquid tank and feeds the liquid in the liquid tank. The liquid feeding part in the form will be described.

　図１０Ａおよび図１０Ｂは、第１実施形態と同様に９の液槽を備えた第３実施形態の液体流路装置１０Ｃについて、送液部Ｐ１’を有する計量槽１４ｃを含む要部について示している。
　この例では、基板１１Ｂは、外層１１ｅと、その内側に積層した中間層１１ｆと、その内側に積層した内層１１ｇの３層から構成されている。
　内層１１ｇには、液槽（この例では計量槽１４ｃのみ図示）の上部と、液体流路１２と、第１凸部１５と、第２凸部１６とが形成されている。
　中間層１１ｆには、液槽の下部が形成されている。また、この中間層１１ｆは、液体流路１２の底部を構成している。
外層１１ｅは、基板１１Ｂの最も外側に配置され、液槽の底部を構成している。この例では、液槽の底部を矢印Ｃ’で示すように外側から押圧する操作により、液槽の底部を構成する外層１１ｅが内側に撓んで、計量槽１４ｃの内容積が小さくなることにより、送液部Ｐ１’が作動する。 10A and 10B show the main part including the measuring tank 14c having the liquid feeding part P1 ′ in the liquid channel device 10C of the third embodiment provided with nine liquid tanks as in the first embodiment. Yes.
In this example, the substrate 11B is composed of three layers: an outer layer 11e, an intermediate layer 11f stacked on the inner side, and an inner layer 11g stacked on the inner side.
In the inner layer 11g, an upper portion of a liquid tank (only the measuring tank 14c is shown in this example), a liquid flow path 12, a first convex portion 15, and a second convex portion 16 are formed.
A lower part of the liquid tank is formed in the intermediate layer 11f. The intermediate layer 11 f constitutes the bottom of the liquid flow path 12.
The outer layer 11e is disposed on the outermost side of the substrate 11B and constitutes the bottom of the liquid tank. In this example, the operation of pressing the bottom of the liquid tank from the outside as indicated by the arrow C ′ causes the outer layer 11e constituting the bottom of the liquid tank to bend inward, and the internal volume of the measuring tank 14c is reduced. The liquid feeding part P1 ′ is activated.

　さらにこの例では、図１１にも示すように、内層１１ｇに逆流防止部としての堰板１８が形成されている。この例の堰板１８は、計量槽１４ｃと計量槽１４ｃの上流側の液体流路１２との境界部分において、堰板１８の先端１８ａが下流側に傾くように、基端１８ｂが液体流路１２の一方の側壁に固定されていて、先端１８ａや両側端は固定されていない。
　そのため、上流の図示略のろ過槽から計量槽１４ｃへ液体が送液される場合には、液体は堰板１８の先端１８ａを超えて計量槽１４ｃへと流入することができる。一方、計量槽１４ｃの有する送液部Ｐ１’が作動した場合には、堰板１８の作用により計量槽１４ｃ内の液体は下流側にしか送液されず、上流側へは逆流しない。 Furthermore, in this example, as shown also in FIG. 11, the weir plate 18 as a backflow prevention part is formed in the inner layer 11g. In this example, the dam plate 18 has a base end 18b at the boundary between the measuring tank 14c and the liquid channel 12 on the upstream side of the measuring tank 14c so that the distal end 18a of the dam plate 18 is inclined downstream. 12 is fixed to one side wall, and the tip 18a and both side ends are not fixed.
Therefore, when the liquid is sent from the upstream unillustrated filtration tank to the measuring tank 14 c, the liquid can flow into the measuring tank 14 c beyond the tip 18 a of the weir plate 18. On the other hand, when the liquid feeding part P1 ′ of the measuring tank 14c is activated, the liquid in the measuring tank 14c is fed only to the downstream side by the action of the weir plate 18 and does not flow backward to the upstream side.

　この例の基板１１Ｂは、図１２に示すようにして製造することができる。
まず、内層１１ｇをなすシート１１ｇ’を用意し、このシート１１ｇ’において、液体流路１２に対応する箇所を線状に打ち抜くとともに、液槽の上部に相当する部分を孔状に打ち抜く。また、この際、第１凸部１５と第２凸部１６となる部分は打ち抜かず、残しておき、その後、第２凸部１６の高さが第１凸部１５よりも低くなるように、研磨などにより第２凸部の高さを調節しておく。また、この例では、堰板１８も内層１１ｇから形成されているため、このシート１１ｇ’において堰板１８となる部分も打ち抜かずに残しておく。
　一方、中間層１１ｆをなすシート１１ｆ’を用意し、このシート１１ｆ’において、計量槽１４ｃなどの各液槽の下部に対応する箇所を孔状に打ち抜く。
ついで、外層１１ｅをなすシート１１ｅ’を用意し、これに中間層１１ｆをなすシート１１ｆ’と、内層１１ｇをなすシート１１ｇ’を積層し、接着することにより、基板１１Ｂを製造することができる。 The substrate 11B of this example can be manufactured as shown in FIG.
First, a sheet 11g ′ forming an inner layer 11g is prepared. In this sheet 11g ′, a portion corresponding to the liquid flow path 12 is punched in a linear shape, and a portion corresponding to the upper portion of the liquid tank is punched in a hole shape. Also, at this time, the portions that become the first convex portion 15 and the second convex portion 16 are not punched out and are left behind, and then the height of the second convex portion 16 is lower than the first convex portion 15. The height of the second convex portion is adjusted by polishing or the like. Further, in this example, since the barrier plate 18 is also formed from the inner layer 11g, the portion that becomes the barrier plate 18 in the sheet 11g ′ is left without being punched.
On the other hand, a sheet 11f ′ forming the intermediate layer 11f is prepared, and in this sheet 11f ′, a portion corresponding to the lower part of each liquid tank such as the measuring tank 14c is punched out into a hole shape.
Next, a sheet 11e ′ forming the outer layer 11e is prepared, and a sheet 11f ′ forming the intermediate layer 11f and a sheet 11g ′ forming the inner layer 11g are laminated and bonded to each other, whereby the substrate 11B can be manufactured.

　各シート１１ｅ’、１１ｆ’、１１ｇ’の材質としては、第１実施形態で例示した基板１１Ａの材質の中などから選択することができる。特にシート１１ｅ’は、液槽の底部を構成し、送液部の作動時には外側から押圧されるものであるため、可撓性を有するものを使用する必要がある。
また、シート１１ｇ’の厚さは、形成される液体流路１２の深さに相当し、シート１１ｇ’とシート１１ｆ’の厚さの和は液槽の総深さに相当する。よって、液槽や液体流路１２に求められる深さを考慮して、これらシート１１ｆ’およびシート１１ｇ’の厚みを決定する。液槽の深さは、要求される容積などに応じて適宜設定すればよい。また、液体流路１２の好適な深さは、第１実施形態と同様の範囲である。一方、シート１１ｅ’は上述のように送液部の作動時には撓む必要があるため、材質にもよるが、具体的には、２０～３００μｍとすることが好適である。 The material of each of the sheets 11e ′, 11f ′, and 11g ′ can be selected from the materials of the substrate 11A exemplified in the first embodiment. In particular, since the sheet 11e ′ constitutes the bottom of the liquid tank and is pressed from the outside when the liquid feeding section is operated, it is necessary to use a flexible sheet.
Further, the thickness of the sheet 11g ′ corresponds to the depth of the liquid flow path 12 to be formed, and the sum of the thicknesses of the sheet 11g ′ and the sheet 11f ′ corresponds to the total depth of the liquid tank. Therefore, the thicknesses of the sheet 11f ′ and the sheet 11g ′ are determined in consideration of the depth required for the liquid tank and the liquid channel 12. What is necessary is just to set the depth of a liquid tank suitably according to the volume etc. which are requested | required. Moreover, the suitable depth of the liquid flow path 12 is the same range as 1st Embodiment. On the other hand, since the sheet 11e ′ needs to bend when the liquid feeding section is operated as described above, specifically, it is preferably 20 to 300 μm although it depends on the material.

　なお、基板１１Ｂは、シート１１ｅ’とシート１１ｇ’とが積層してなり、外層１１ｅと内層１１ｇとの２層から構成されてもよい。この場合、液体流路１２と液槽の深さは同じとなる。 The substrate 11B may be composed of a laminate of a sheet 11e 'and a sheet 11g', and may be composed of two layers, an outer layer 11e and an inner layer 11g. In this case, the depth of the liquid channel 12 and the liquid tank are the same.

　また、この形態の液体流路装置１０Ｃにおいて、より送液部Ｐ１’を効果的に作用させるために、図１３に示すように、計量槽１４ｃに対応する部分の基板１１Ｂの外層１１ｅ、すなわち、計量槽１４ｃの底部を外方に膨出させてもよい。このように膨出させることによって、送液部Ｐ１’を作動させる際にこの部分を内側に押圧すると、計量槽１４ｃの内容積をより小さくでき、その結果、計量槽１４ｃ中の液体をより効果的に送液することが可能となる。 Further, in the liquid flow path device 10C of this embodiment, in order to make the liquid feeding part P1 ′ act more effectively, as shown in FIG. 13, the outer layer 11e of the substrate 11B corresponding to the weighing tank 14c, that is, The bottom of the measuring tank 14c may be bulged outward. By bulging in this way, when this portion is pressed inward when operating the liquid feeding part P1 ′, the internal volume of the measuring tank 14c can be made smaller, and as a result, the liquid in the measuring tank 14c can be made more effective. Liquid can be fed.

　このような基板１１Ｂに、第１実施形態と同様の構成の蓋板１３を設けることにより、第３実施形態の液体流路装置１０Ｃを得ることができる。すなわち、この例でも、図１０Ａおよび図１０Ｂにも示すように、計量槽１４ｃに対応する部分の蓋板１３においては、強粘着層１３ｂと第２基材層１３ｃとの間は離間しているのではなく、スペーサ部材１７が介在してスペーサ部材１７と強粘着層１３ｂとが粘着し、層間が密に構成されている。そのため、計量槽１４ｃの底部を外側から押圧して撓ませ、送液部Ｐ１’を作動させた際には、計量槽１４ｃの内容積が小さくなり、送液部としての作用が発現する。ここで仮に、強粘着層１３ｂと第２基材層１３ｃとの間が離間し、スペーサ部材１７が介在しておらず、層間が密でないと、計量槽１４ｃの底部を外側から押圧した際に、第２基材層１３ｃと弱粘着層１３ｄとが計量槽１４ｃの内圧により外方に撓んでしまい、計量槽１４ｃの内容積が小さくならず、送液部としての作用が発現しない可能性がある。 By providing the cover plate 13 having the same configuration as that of the first embodiment on such a substrate 11B, the liquid flow path device 10C of the third embodiment can be obtained. That is, also in this example, as shown in FIGS. 10A and 10B, the strong adhesive layer 13b and the second base material layer 13c are separated from each other in the cover plate 13 corresponding to the weighing tank 14c. Instead, the spacer member 17 is interposed, the spacer member 17 and the strong adhesion layer 13b adhere to each other, and the layers are densely configured. Therefore, when the bottom of the measuring tank 14c is pressed and bent from the outside and the liquid feeding part P1 'is operated, the internal volume of the measuring tank 14c is reduced, and the action as the liquid feeding part is exhibited. Here, if the strong adhesion layer 13b and the second base material layer 13c are separated from each other, the spacer member 17 is not interposed, and the interlayer is not dense, the bottom of the measuring tank 14c is pressed from the outside. The second base material layer 13c and the weak adhesive layer 13d are bent outward due to the internal pressure of the measuring tank 14c, the internal volume of the measuring tank 14c is not reduced, and the function as the liquid feeding unit may not be exhibited. is there.

　このような液体流路装置１０Ｃにおいては、特に基板１１Ｂが、外層１１ｅと中間層１１ｆと内層１１ｇの３層、または、外層１１ｅと内層１１ｇの２層から構成され、液槽、液体流路１２、堰板１８、第１凸部１５および第２凸部１６は、中間層１１ｆを構成するシート１１ｆ’や内層１１ｇを構成するシート１１ｇ’を打ち抜くことで形成されている。そのため、一枚の平板からなる基板に対して、液槽や液体流路を例えばフォトリソグラフィなどで形成する方法、射出成形などで液槽や液体流路の形成された基板を成形する方法などにくらべて、低い製造コストで簡便に、液槽などを形成でき、大量生産も可能となる。 In such a liquid channel device 10C, in particular, the substrate 11B is composed of three layers of the outer layer 11e, the intermediate layer 11f and the inner layer 11g, or two layers of the outer layer 11e and the inner layer 11g. The barrier plate 18, the first convex portion 15 and the second convex portion 16 are formed by punching out the sheet 11f ′ constituting the intermediate layer 11f and the sheet 11g ′ constituting the inner layer 11g. Therefore, for a substrate made of a single flat plate, a method for forming a liquid tank or a liquid flow path by, for example, photolithography, a method for forming a substrate in which a liquid tank or a liquid flow path is formed by injection molding, etc. Compared with a low manufacturing cost, a liquid tank etc. can be formed easily and mass production is also possible.

　なお、以上の説明した第１～第３実施形態においては、基板１１Ａ、１１Ｂの片面のみに液体流路１２が形成された液体流路装置１０Ａ、１０Ｂ、１０Ｃを例示したが、基板１１Ａ、１１Ｂの両面に液体流路１２が形成されてもよい。
　また、各液槽に設けられる、開閉可能な連通孔の形態には制限はなく、蓋板に形成された連通孔に、嵌め込み式のキャップを抜き差しすることで、連通孔を開通、閉止できる形態などでもよいが、液体流路１２に設けられる開通部Ｓ１～Ｓ７および閉止部Ｔ１と同様の構成の開通部と閉止部とを設けてもよい。
　また、第１実施形態のように、基板１１Ａを一枚の平板から構成した場合でも、液槽の底部を押圧することで作動する送液部を液槽に設けてもよいし、第３実施形態のように、基板１１Ｂを複数層から構成した場合でも、液槽に対応する蓋板１３を押圧することで作動する送液部を液槽に設けてもよい。さらに、以上の説明では、閉止部および開通部を備えた液体流路装置を例に挙げたため、蓋板１３として、第１基材層１３ａ、強粘着層１３ｂ、第２基材層１３ｃ、弱粘着層１３ｄ、スペーサ部材１７から構成されるものを例示したが、送液部の作動のためには、蓋板１３をこのように複数の層から構成する必要はなく、単層からなる蓋板であってもよい。
　また、以上の例においては、液体を流通させるために、重力の作用と送液部の作用とを利用した形態について示したが、さらに、液体流路１２、液槽の一部、またはこれらの両方を加熱して液体流路１２や液槽内の空気を膨張させたり、液体流路１２の一部に酸素吸収剤（酸化しやすい鉄粉など）を封入しておき、液体流路１２内の酸素を吸収することで液体流路１２内を減圧にしたりして、液体を移動させ、流通させる方法などを併用してもよい。 In the first to third embodiments described above, the liquid flow path apparatuses 10A, 10B, and 10C in which the liquid flow path 12 is formed only on one surface of the substrates 11A and 11B are exemplified. However, the substrates 11A and 11B are exemplified. The liquid channel 12 may be formed on both sides of the liquid crystal.
Moreover, there is no restriction | limiting in the form of the communication hole which can be opened and closed provided in each liquid tank, The form which can open and close a communication hole by inserting and extracting a fitting type cap to the communication hole formed in the cover plate. However, an opening portion and a closing portion having the same configuration as the opening portions S1 to S7 and the closing portion T1 provided in the liquid channel 12 may be provided.
Further, as in the first embodiment, even when the substrate 11A is composed of a single flat plate, a liquid feeding section that operates by pressing the bottom of the liquid tank may be provided in the liquid tank. As in the embodiment, even when the substrate 11B is composed of a plurality of layers, a liquid feeding section that operates by pressing the cover plate 13 corresponding to the liquid tank may be provided in the liquid tank. Furthermore, in the above description, since the liquid flow path device provided with the closing part and the opening part is given as an example, the first base material layer 13a, the strong adhesive layer 13b, the second base material layer 13c, the weak base material are used as the cover plate 13. Although an example composed of the adhesive layer 13d and the spacer member 17 has been illustrated, the lid plate 13 does not need to be composed of a plurality of layers in this way for the operation of the liquid feeding unit, and is a single layer lid plate. It may be.
Moreover, in the above example, in order to distribute | circulate the liquid, it showed about the form using the effect | action of gravity and the effect | action of a liquid feeding part, Furthermore, the liquid flow path 12, a part of liquid tank, or these Both are heated to expand the air in the liquid flow path 12 and the liquid tank, or an oxygen absorbent (such as iron powder that easily oxidizes) is sealed in a part of the liquid flow path 12, A method of reducing the pressure in the liquid flow path 12 by absorbing oxygen and moving and circulating the liquid may be used in combination.

　また、以上の説明では、試料投入槽１４ａに試料を注入する方法として、シリンジの針を蓋板１３に突き刺す方法を例示しているが、例えば、あらかじめ蓋板１３に試料注入孔を形成しておき、そこから試料を注入してもよい。その場合、試料注入孔には保護テープを被せておき、シリンジを保護テープに突き刺すことで注入してもよいし、保護テープを剥がして試料注入孔にシリンジを挿入して注入してもよい。 In the above description, as a method of injecting the sample into the sample loading tank 14a, a method of piercing the needle of the syringe into the cover plate 13 is exemplified. For example, a sample injection hole is formed in the cover plate 13 in advance. Alternatively, the sample may be injected therefrom. In that case, the sample injection hole may be covered with a protective tape and injected by piercing the syringe with the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.

　また、各液槽に設けられる連通孔は、液体流路装置１１Ａ、１１Ｂの使用前には封止され、使用時に開通されることがコンタミネーション防止の点などから好ましい。よって、連通孔の手前に、液体流路１２に設けられる開通部Ｓ１～１４と同様の構成の開通部を設けてもよい。
　また、以上の説明においては、液体を流通させるために、液体流路装置１０Ａでは重力を利用し、液体流路装置１０Ｂでは遠心力を利用した方法をそれぞれ説明したが、これらに限定されず、例えば、液体流路１２の一部を加圧したり、液体流路１２の一部を加熱して液体流路１２内の空気を膨張させたり、液体流路１２の一部に酸素吸収剤（酸化しやすい鉄粉など）を封入しておき、液体流路１２内の酸素を吸収することで液体流路１２内を減圧にしたりして、液体を移動させ、流通させる方法なども採用できる。または、液体流路１２の一部ではなく、計量槽１４を加圧、加熱、減圧してもよいし、場合によっては、液体流路１２と計量槽１４の両方を加圧、加熱、減圧してもよい。 Moreover, it is preferable from the point of contamination prevention etc. that the communicating hole provided in each liquid tank is sealed before use of liquid flow-path apparatus 11A, 11B, and is opened at the time of use. Therefore, an opening portion having the same configuration as the opening portions S1 to S14 provided in the liquid channel 12 may be provided before the communication hole.
In the above description, in order to circulate the liquid, the liquid channel device 10A uses gravity and the liquid channel device 10B uses centrifugal force. However, the method is not limited thereto. For example, a part of the liquid channel 12 is pressurized, a part of the liquid channel 12 is heated to expand the air in the liquid channel 12, or an oxygen absorbent (oxidation) is added to a part of the liquid channel 12. It is also possible to adopt a method in which a liquid is moved and circulated by enclosing an easy-to-treat iron powder or the like and absorbing the oxygen in the liquid channel 12 to reduce the pressure in the liquid channel 12. Alternatively, the measuring tank 14 may be pressurized, heated, or depressurized instead of a part of the liquid channel 12, or depending on the case, both the liquid channel 12 and the measuring tank 14 may be pressurized, heated, or depressurized. May be.

　液体流路装置１０Ａ、１０Ｂを流通させる試料および試薬としては、特に制限はなく、医療分野、環境分野などで従来から採用されている試料と試薬とを適宜組み合わせて使用することができる。例えば、医療分野おいては、試料として、血液（全血）、血漿、血清、バフィーコート、尿、糞便、唾液、喀痰などの生体由来のもの、ウィルス、細菌、カビ、酵母、動植物の細胞などが挙げられる。　
　また、これらから単離したＤＮＡまたはＲＮＡを用いてもよいし、これらに対して何らかの前処理、希釈などが施されたものを試料としてもよい。試薬としては、試料中に存在する抗原を分析する場合には、それに対する抗体を含有する試薬が好ましい。
　また、液体流路装置１０Ａ、１０Ｂで調製された測定液の検出分析部としては、従来公知の光学的手段、電気的手段などを適宜採用することができる。 There are no particular limitations on the sample and reagent through which the liquid flow path devices 10A and 10B are circulated, and a sample and a reagent that have been conventionally used in the medical field, the environmental field, and the like can be used in appropriate combination. For example, in the medical field, samples (eg, blood (whole blood), plasma, serum, buffy coat, urine, feces, saliva, sputum, etc.), viruses, bacteria, mold, yeast, animal and plant cells, etc. Is mentioned.
In addition, DNA or RNA isolated from these may be used, and a sample obtained by subjecting them to any pretreatment or dilution may be used. As a reagent, when an antigen present in a sample is analyzed, a reagent containing an antibody against the antigen is preferable.
Moreover, conventionally known optical means, electrical means, and the like can be appropriately employed as the detection / analysis unit for the measurement liquid prepared by the liquid flow path apparatuses 10A and 10B.

　［第４実施形態］
　図１４は第４実施形態の液体流路装置の一実施形態を概略的に示す平面透視図、図１５は図１４の液体流路装置の一部を拡大した平面透視図、図１６は図１５のＩ－Ｉ’線に沿う断面図である。
　この液体流路装置１１０は、平板からなる四角形の基板１１１の片面に、試料および試薬の少なくとも一方からなる液体が流通する溝状の液体流路１１２と、液体流路１１２の端部や途中において液体が溜まる複数（この例では９）の液槽（１１４ａ～１１４ｉ）とが形成され、基板１１１の液体流路１１２が形成された側の流路形成面１１２ａに蓋板１１３が積層して構成されている。この液体流路装置１１０においては、図１４中の上端部側が上方に、下端部側が下方に位置するように液体流路装置１１０を立てた場合には、液体流路１１２の上流側の端部から下流側の端部に向けて矢印Ｆ方向に試料が重力により流通し、その途中で試料に対して各種の処理や試薬との混合がなされ、各種検出、分析に供される測定液が調製される。 [Fourth Embodiment]
14 is a plan perspective view schematically showing an embodiment of the liquid channel device of the fourth embodiment, FIG. 15 is a plan perspective diagram showing an enlarged part of the liquid channel device of FIG. 14, and FIG. It is sectional drawing which follows the II 'line | wire.
The liquid channel device 110 includes a groove-like liquid channel 112 through which a liquid consisting of at least one of a sample and a reagent flows on one side of a rectangular substrate 111 made of a flat plate, and an end portion or a middle of the liquid channel 112. A plurality of (9 in this example) liquid tanks (114a to 114i) in which liquid is accumulated are formed, and a cover plate 113 is laminated on the flow path forming surface 112a on the side where the liquid flow path 112 of the substrate 111 is formed. Has been. In the liquid channel device 110, when the liquid channel device 110 is erected so that the upper end side in FIG. The sample flows by gravity in the direction of arrow F toward the downstream end from the center, and the sample is mixed with various treatments and reagents in the middle to prepare measurement solutions for various detections and analyzes. Is done.

　すなわち、液体流路１１２の上流側の端部には、投入された試料が溜まる試料投入槽１１４ａが設けられ、この試料投入槽１１４ａの下流には、試料投入槽１１４ａから流通してきた試料に対してろ過処理が施される図示略のフィルタが内蔵されたろ過槽１１４ｂが設けられている。
　ろ過槽１１４ｂの下流には、ろ過処理された試料を一定量計量する計量槽１１４ｃが設けられている。この例の計量槽１１４ｃには、オーバーフロー流路１１２ｄとその下流に設けられた廃液槽１１４ｄとからなるオーバーフロー部が備えられている。そのため、計量槽１１４ｃで一定量を超えた試料はオーバーフローしてオーバーフロー流路１１２ｄを流れ、廃液槽１１４ｄに流入し、その結果、計量槽１１４ｃでは、一定量の試料が計量できる。 That is, at the upstream end of the liquid channel 112, a sample loading tank 114a in which the loaded sample is stored is provided, and downstream of the sample loading tank 114a, the sample flowing from the sample loading tank 114a is provided. A filtration tank 114b containing a filter (not shown) to be filtered is provided.
A measuring tank 114c for measuring a certain amount of the filtered sample is provided downstream of the filtering tank 114b. The measuring tank 114c of this example is provided with an overflow portion comprising an overflow channel 112d and a waste liquid tank 114d provided downstream thereof. Therefore, the sample exceeding a certain amount in the measuring tank 114c overflows and flows through the overflow channel 112d and flows into the waste liquid tank 114d. As a result, a certain amount of sample can be measured in the measuring tank 114c.

　計量槽１１４ｃの下流には、計量槽１１４ｃで計量された試料と、あらかじめ第１試薬槽１１４ｅに所定量封入されている液体の第１試薬とが混合される第１混合槽１１４ｆが設けられ、第１混合槽１１４ｆの下流には、第１混合槽１１４ｆで調製された中間調製液と、あらかじめ第２試薬槽１１４ｇに所定量封入されている液体の第２試薬とが混合される第２混合槽１１４ｈが設けられている。
　そして、第２混合槽１１４ｈの下流（液体流路１１２の下流側の端部）には測定槽１１４ｉが設けられ、第２混合槽１１４ｈで調製された測定液がここに貯留され、図示略の検出分析部により、各種成分の検出や分析が行われる。
　なお、各液槽には、必要に応じて、大気と連通する開閉可能な図示略の連通孔が設けられる。 Downstream of the measuring tank 114c, a first mixing tank 114f is provided in which the sample weighed in the measuring tank 114c and the liquid first reagent sealed in a predetermined amount in the first reagent tank 114e are mixed. Downstream of the first mixing tank 114f is a second mixing in which the intermediate preparation liquid prepared in the first mixing tank 114f and the liquid second reagent sealed in a predetermined amount in the second reagent tank 114g are mixed. A tank 114h is provided.
A measuring tank 114i is provided downstream of the second mixing tank 114h (the end on the downstream side of the liquid channel 112), and the measurement liquid prepared in the second mixing tank 114h is stored here, and is not shown. The detection analysis unit detects and analyzes various components.
Each liquid tank is provided with a communication hole (not shown) that can be opened and closed and communicates with the atmosphere as needed.

　この液体流路装置１１０の基板１１１は、図１６に示すように、外層１１１ａと、その内側に積層した中間層１１１ｂと、その内側に積層した内層１１１ｃの３層から構成されている。
　内層１１１ｃには、液槽（図３には試料投入槽１１４ａとろ過槽１１４ｂのみ図示）の上部（液槽の蓋板１１３側の部分。）と、液体流路１１２とが形成されている。中間層１１１ｂには、液槽の下部（前記上部以外であって、液槽の底部側の部分。）が形成されている。また、この中間層１１１ｂは、内層１１１ｃ側の面が液体流路１１２の底部１１２ｂを構成している。外層１１１ａは、基板１１１の最も外側に配置され、その中間層１１１ｂ側の面が液槽の底部を構成している。 As shown in FIG. 16, the substrate 111 of the liquid channel device 110 includes three layers: an outer layer 111a, an intermediate layer 111b stacked on the inner side, and an inner layer 111c stacked on the inner side.
In the inner layer 111c, an upper portion (portion on the lid 113 side of the liquid tank) of the liquid tank (only the sample charging tank 114a and the filtration tank 114b are shown in FIG. 3) and the liquid channel 112 are formed. In the intermediate layer 111b, a lower part of the liquid tank (a part other than the upper part and on the bottom side of the liquid tank) is formed. Further, in the intermediate layer 111 b, the surface on the inner layer 111 c side forms the bottom portion 112 b of the liquid channel 112. The outer layer 111a is disposed on the outermost side of the substrate 111, and the surface on the intermediate layer 111b side forms the bottom of the liquid tank.

　そして、この液体流路装置１１０は、液体流路１１２の一部を閉止状態から開通状態にする開通部Ｓ１１～Ｓ１７と、開通状態から閉止状態にする閉止部Ｔ１１とを有している。
　この例では、開通部Ｓ１１～Ｓ１７は、試料投入槽１１４ａとろ過槽１１４ｂとの間、ろ過槽１１４ｂと計量槽１１４ｃとの間、計量槽１１４ｃと第１混合槽１１４ｆとの間、第１混合槽１１４ｆと第２混合槽１１４ｈとの間、第１試薬槽１１４ｅと第１混合槽１１４ｆとの間、第２試薬槽１１４ｇと第２混合槽１１４ｈとの間、第２混合槽１１４ｈと測定槽１１４ｉとの間の各液体流路１１２にそれぞれ１ずつ設けられている。
　一方、閉止部Ｔ１１は、ろ過槽１１４ｂと計量槽１１４ｃとの間の液体流路１１２において、開通部Ｓ１２よりも下流側に設けられている。 The liquid flow path device 110 includes opening portions S11 to S17 that make a part of the liquid flow path 112 open from the closed state, and a closing portion T11 that changes the open state to the closed state.
In this example, the opening sections S11 to S17 include the first mixing tank 114a and the filtering tank 114b, the filtering tank 114b and the measuring tank 114c, the measuring tank 114c and the first mixing tank 114f, Between the tank 114f and the second mixing tank 114h, between the first reagent tank 114e and the first mixing tank 114f, between the second reagent tank 114g and the second mixing tank 114h, and between the second mixing tank 114h and the measuring tank One is provided in each liquid flow path 112 between 114i.
On the other hand, the closing part T11 is provided downstream of the opening part S12 in the liquid channel 112 between the filtration tank 114b and the measuring tank 114c.

　開通部Ｓ１１～Ｓ１７は、図１６にＳ１１およびＳ１２を例示して説明するように、液体流路１１２内に、液体流路１１２の一部を塞ぐように配置されて液体の流れを封止し、この部分を閉止状態とする樹脂製の栓体１１５により形成されている。この栓体１１５は、塑性変形が可能な樹脂からなり、栓体１１５が配置されている部分の蓋板１１３を外側から押圧する操作により塑性変形して、液体流路１１２を閉止状態から開通状態にする。
　具体的には、図１７Ａおよび図１７Ｂに開通部Ｓ１１を例に挙げて示すように、開通部Ｓ１１を構成する栓体１１５を蓋板１１３の外側から矢印Ａで示すように押圧して荷重を加えた場合、図１７Ａに示すように蓋板１１３が撓み、蓋板１１３に接している栓体１１５が押し潰されて扁平に塑性変形する。そして、その後に荷重を取り去ると、図１７Ｂに示すように、蓋板１１３はその復元力により元の状態に復元するが、栓体１１５は扁平に変形したままで復元しない。その結果、栓体１１５と蓋板１１３との間が新たに離間し、ここを液体が流通できるようになる。
　このように開通部Ｓ１１～Ｓ１７においては、栓体１１５を蓋板１１３の外側から押圧して荷重を加えた後、この荷重を取り去る押圧操作によって、元々は密着していた栓体１１５と蓋板１１３との間が離間し、その結果、この部分の液体流路１１２が閉止状態から開通状態となる。 The opening portions S11 to S17 are arranged in the liquid channel 112 so as to block a part of the liquid channel 112 and seal the flow of the liquid, as described with reference to S11 and S12 in FIG. In addition, it is formed by a resin plug 115 that closes this portion. The plug 115 is made of a resin that can be plastically deformed, and is plastically deformed by pressing the lid plate 113 where the plug 115 is disposed from the outside, so that the liquid channel 112 is opened from the closed state. To.
Specifically, as shown in FIG. 17A and FIG. 17B with the opening portion S11 as an example, the plug body 115 constituting the opening portion S11 is pressed from the outside of the cover plate 113 as indicated by an arrow A to apply a load. When added, the cover plate 113 bends as shown in FIG. 17A, and the plug 115 that is in contact with the cover plate 113 is crushed and plastically deformed flat. Then, when the load is removed thereafter, as shown in FIG. 17B, the cover plate 113 is restored to its original state by its restoring force, but the plug body 115 remains flat and does not restore. As a result, the plug body 115 and the cover plate 113 are newly separated from each other so that liquid can flow therethrough.
In this way, in the opening portions S11 to S17, the stopper 115 is pressed from the outside of the lid plate 113 and a load is applied, and then the stopper 115 and the lid plate that were originally in close contact with each other by a pressing operation to remove the load. As a result, the liquid flow path 112 in this portion is changed from the closed state to the open state.

　一方、この液体流路装置１１０の閉止部Ｔ１１は、図１８にも示すように、液体流路１１２から分岐して基板１１１に形成された封止材料供給槽１１６と、この封止材料供給槽１１６に充填されたペースト状の封止材料１１７とを有している。
　封止材料１１７は、図１９Ａの断面図および図１９Ｂの平面図に示すように、封止材料供給槽１１６に対応する部分の蓋板１１３を外側から押圧する操作により、封止材料供給槽１１６と液体流路１１２とを繋ぐ供給路１１８を経て、液体流路１１２に押し出され、その部分を開通状態から閉止状態にする。
　具体的には、封止材料供給槽１１６に対応する部分の蓋板１１３を矢印Ｂで示すように外側から押圧して荷重を加えると、蓋板１１３が撓む。その結果、矢印Ｃで示すように、封止材料供給槽１１６に充填されている封止材料１１７が供給路１１８を経て液体流路１１２に押し出される。その結果、押し出された封止材料１１７により、液体流路１１２は閉塞され、液体はこの部分を流通できなくなる。
　なお、開通状態から閉止状態にできる液体流路装置の場合では、封止材料供給槽１１６と液体流路１１２とは供給路１１８を介して連通しているが、この場合においても供給路１１８が形成されず、直に封止材料供給槽１１６と液体流路１１２とが連通している形態でもよい。 On the other hand, as shown in FIG. 18, the closing portion T11 of the liquid flow path device 110 includes a sealing material supply tank 116 that is branched from the liquid flow path 112 and formed on the substrate 111, and the sealing material supply tank. 116 and a paste-like sealing material 117 filled in 116.
As shown in the cross-sectional view of FIG. 19A and the plan view of FIG. 19B, the sealing material 117 is pressed by pressing the lid plate 113 corresponding to the sealing material supply tank 116 from the outside. The liquid channel 112 is pushed through a supply channel 118 that connects the liquid channel 112 and the liquid channel 112, and the portion is changed from the open state to the closed state.
Specifically, when a cover plate 113 corresponding to the sealing material supply tank 116 is pressed from the outside as indicated by an arrow B and a load is applied, the cover plate 113 is bent. As a result, as shown by an arrow C, the sealing material 117 filled in the sealing material supply tank 116 is pushed out to the liquid flow path 112 through the supply path 118. As a result, the liquid flow path 112 is blocked by the extruded sealing material 117, and the liquid cannot flow through this portion.
In the case of the liquid flow path device that can be changed from the open state to the closed state, the sealing material supply tank 116 and the liquid flow path 112 communicate with each other via the supply path 118. A form in which the sealing material supply tank 116 and the liquid flow path 112 are directly communicated with each other may be used.

　このような液体流路装置１１０を用いて、測定液を調製する具体的な方法としては、まず、この液体流路装置１１０を試料投入槽１１４ａ側が上方に、測定槽１１４ｉ側が下方に位置するように立てて、液体が重力によって上流側から下流側に流れやすい状態とする。
　ついで、試料をシリンジなどにサンプリングし、このシリンジの針を試料投入槽１１４ａに対応する部分の蓋板１１３に突き刺して、試料投入槽１１４ａに試料を注入する。その後、試料投入槽１１４ａとろ過槽１１４ｂとの間に設けられた開通部Ｓ１１、すなわち栓体１１５を蓋板１１３の外側から押圧して塑性変形させ、この部分の液体流路１１２を開通状態とし、試料を重力によりろ過槽１１４ｂまで導入する。
　この際、押圧する操作は、作業者が指で押す手動により行ってもよいし、押圧位置がＸＹ座標としてあらかじめプログラムされている押圧装置などを使用して、所定の位置を押すようにしてもよい。 As a specific method for preparing a measurement liquid using such a liquid channel device 110, first, the liquid channel device 110 is positioned so that the sample introduction tank 114a side is positioned above and the measurement tank 114i side is positioned below. Therefore, the liquid is likely to flow from the upstream side to the downstream side by gravity.
Next, the sample is sampled in a syringe or the like, and the needle of this syringe is pierced into the cover plate 113 corresponding to the sample loading tank 114a, and the sample is injected into the sample loading tank 114a. Thereafter, the opening portion S11 provided between the sample introduction tank 114a and the filtration tank 114b, that is, the stopper 115 is pressed from the outside of the cover plate 113 to be plastically deformed, and the liquid flow path 112 in this portion is opened. The sample is introduced to the filtration tank 114b by gravity.
At this time, the pressing operation may be performed manually by an operator pressing with a finger, or a predetermined position may be pressed using a pressing device in which the pressing position is programmed in advance as XY coordinates. Good.

　ついで、ろ過槽１１４ｂでろ過処理がなされた後、ろ過槽１１４ｂと計量槽１１４ｃとの間に設けられた開通部Ｓ１２の栓体１１５についても同様に塑性変形させ、この部分の液体流路１１２を開通状態とし、試料を重力により計量槽１１４ｃに導入する。
　ついで、計量槽１１４ｃにおいて、導入された液体がオーバーフローし始めたことを確認後、ろ過槽１１４ｂと計量槽１１４ｃとの間に設けられた閉止部Ｔ１１を作動させて、この部分の液体流路１１２を閉止状態とする。具体的には、封止材料供給槽１１６に対応する部分の蓋板１１３を矢印Ｂで示すように押圧して荷重を加え、封止材料供給槽１１６に充填されている封止材料１１７を液体流路１１２まで押し出し、この部分の液体流路１１２を閉塞する。
　このようにして、計量槽１１４ｃに上流側からの液体がさらに流入するのを停止させてから、計量槽１１４ｃの下流に設けられた開通部Ｓ１３を作動させて、計量槽１１４ｃで計量された試料を第１混合槽１１４ｆに導入する。 Next, after the filtration process is performed in the filtration tank 114b, the plug body 115 of the opening portion S12 provided between the filtration tank 114b and the measuring tank 114c is also plastically deformed, and the liquid flow path 112 of this portion is made to be the same. The open state is established, and the sample is introduced into the measuring tank 114c by gravity.
Next, after confirming that the introduced liquid has started to overflow in the measuring tank 114c, the closing portion T11 provided between the filtration tank 114b and the measuring tank 114c is operated, and the liquid flow path 112 of this portion is operated. Is closed. Specifically, the cover plate 113 corresponding to the sealing material supply tank 116 is pressed as indicated by an arrow B to apply a load, and the sealing material 117 filled in the sealing material supply tank 116 is liquid. The liquid channel 112 is pushed out to the flow channel 112 and the liquid flow channel 112 in this portion is closed.
In this way, after stopping the further flow of the liquid from the upstream side into the measuring tank 114c, the opening portion S13 provided downstream of the measuring tank 114c is operated, and the sample measured in the measuring tank 114c. Is introduced into the first mixing tank 114f.

　こうして計量後の試料を第１混合槽１１４ｆに導入する一方で、第１試薬槽１１４ｅと第１混合槽１１４ｆとの間の開通部Ｓ１４についても、栓体を同様に塑性変形させて第１試薬を第１混合槽１１４ｆに導入し、試料と第１試薬とを第１混合槽１１４ｆにおいて混合し、中間調製液を調製する。
　ついで、第１混合槽１１４ｆと第２混合槽１１４ｈとの間の開通部Ｓ１５についても、栓体を同様に塑性変形させて第１混合槽１１４ｆで調製された中間調製液を第２混合槽１１４ｈに導入する。その一方で、第２試薬槽１１４ｇと第２混合槽１１４ｈとの間の開通部Ｓ１６についても、栓体を同様に塑性変形させて第２試薬を第２混合槽１１４ｈに導入する。そして、中間調製液と第２試薬とを第２混合槽１１４ｈにおいて混合し、測定液を調製する。
　ついで、第２混合槽１１４ｈと測定槽１１４ｉとの間の開通部Ｓ１７についても、栓体を同様に塑性変形させて、第２混合槽１１４ｈで調製された測定液を測定槽１１４ｉに導入する。
　そして、測定槽１１４ｉに測定液を導入した後、この液体流路装置１１０ごと検出分析部に供し、目的成分の検出や測定を行う。
　なお、このようにして測定液を調製する過程においては、各液槽に設けられている図示略の連通孔を必要に応じて適宜開閉することにより、液体を流れ易くしたり、流量の正確性を向上させたりする等、液体の流通を制御してもよい。 In this way, while the sample after weighing is introduced into the first mixing tank 114f, the stopper is similarly plastically deformed in the opening portion S14 between the first reagent tank 114e and the first mixing tank 114f by the first reagent. Is introduced into the first mixing tank 114f, and the sample and the first reagent are mixed in the first mixing tank 114f to prepare an intermediate preparation solution.
Next, also in the opening part S15 between the first mixing tank 114f and the second mixing tank 114h, the intermediate preparation liquid prepared in the first mixing tank 114f by plastically deforming the stopper in the same manner is used as the second mixing tank 114h. To introduce. On the other hand, the opening reagent S16 between the second reagent tank 114g and the second mixing tank 114h is also plastically deformed to introduce the second reagent into the second mixing tank 114h. Then, the intermediate preparation solution and the second reagent are mixed in the second mixing tank 114h to prepare a measurement solution.
Next, also in the opening portion S17 between the second mixing tank 114h and the measurement tank 114i, the stopper is similarly plastically deformed, and the measurement liquid prepared in the second mixing tank 114h is introduced into the measurement tank 114i.
And after introduce | transducing a measurement liquid into the measurement tank 114i, it uses for the detection analysis part with this liquid flow-path apparatus 110, and performs detection and measurement of a target component.
In the process of preparing the measurement liquid in this way, it is possible to facilitate the flow of the liquid or to accurately determine the flow rate by appropriately opening and closing communication holes (not shown) provided in each liquid tank as necessary. The flow of the liquid may be controlled, for example, by improving the flow rate.

　このような液体流路装置１１０によれば、液体流路１１２を閉止状態から開通状態にする開通部Ｓ１１～Ｓ１７と、開通状態から閉止状態にする閉止部Ｔ１１とを有するため、液体流路１１２中の液体の流れを制御でき、その結果、精度の高い検出や分析を短時間で行うことができる。
　例えば、この例では、計量槽１１４ｃの上流には閉止部Ｔ１１が設けられ、下流には開通部Ｓ１３が設けられている。そのため、計量槽１１４ｃで試料を短時間で正確に計量して、第１混合槽１１４ｆに導入することができる。ここで仮に、計量槽１１４ｃの下流に開通部Ｓ１３が設けられておらず、この部分の液体流路１１２が常に開通した状態であると、計量中であっても計量槽１１４ｃから試料が連続的に流出してしまい、試料を一定量溜めることができず、計量自体が不可能となる。また、計量槽１１４ｃの上流に閉止部Ｔ１１が設けられていない場合には、ろ過槽１１４ｂを経た試料の全量が計量槽１１４ｃに完全に流入し終わってから、計量槽１１４ｃと第１混合槽１１４ｆの間の開通部Ｓ１３を作動させて、計量された試料を第１混合槽１１４ｆに導入する必要がある。この場合、試料が特に血液などの粘性を有した液体であると、ろ過槽１１４ｂを経た試料の全量が完全に計量槽１１４ｃに流入し終わるまでに時間を要し、短時間での計量が困難となる。その点、この例のように、計量槽１１４ｃの上流側に閉止部Ｔ１１が設けられていると、ろ過槽１１４ｂを経た試料の全量が計量槽１１４ｃに完全に流入し終わらなくても、計量槽１１４ｃにおいて試料がオーバーフローし始めた時点で閉止部Ｔ１１を作動させて、計量槽１１４ｃへの試料のさらなる流入を停止することができ、短時間での正確な計量が可能となる。 According to such a liquid flow path device 110, the liquid flow path 112 includes the opening portions S11 to S17 that make the liquid flow path 112 from the closed state to the open state and the closing portion T11 that makes the liquid flow path 112 from the open state to the closed state. The flow of the liquid inside can be controlled, and as a result, highly accurate detection and analysis can be performed in a short time.
For example, in this example, a closing portion T11 is provided upstream of the measuring tank 114c, and an opening portion S13 is provided downstream. Therefore, the sample can be accurately weighed in the weighing tank 114c in a short time and introduced into the first mixing tank 114f. Here, if the opening portion S13 is not provided downstream of the measuring tank 114c and the liquid flow path 112 in this portion is always open, the sample is continuously transferred from the measuring tank 114c even during measurement. The sample cannot be stored in a certain amount, and measurement itself is impossible. Further, when the closing portion T11 is not provided upstream of the measuring tank 114c, after the entire amount of the sample that has passed through the filtration tank 114b has completely flowed into the measuring tank 114c, the measuring tank 114c and the first mixing tank 114f. It is necessary to operate the opening portion S13 between and introduce the weighed sample into the first mixing tank 114f. In this case, if the sample is a liquid having a viscosity such as blood, it takes time until the entire amount of the sample passing through the filtration tank 114b completely flows into the measurement tank 114c, and it is difficult to measure in a short time. It becomes. In this regard, as in this example, when the closing portion T11 is provided on the upstream side of the measuring tank 114c, even if the entire amount of the sample that has passed through the filtration tank 114b does not completely flow into the measuring tank 114c, the measuring tank When the sample starts to overflow in 114c, the closing portion T11 is operated to stop further inflow of the sample into the measuring tank 114c, and accurate measurement can be performed in a short time.

　また、この例では、第１混合槽１１４ｆと第２混合槽１１４ｈとの間に開通部Ｓ１５が設けられ、第２混合槽１１４ｈと測定槽１１４ｉとの間に開通部Ｓ１７が設けられている。そのため、第１混合槽１１４ｆおよび第２混合槽１１４ｈにおいて、目的の混合や反応が十分に進行してから、これら開通部Ｓ１５、Ｓ１７を開通させ、中間調製液や測定液をそれぞれ第２混合槽１１４ｈや測定槽１１４ｉに導入することができる。よって、混合や反応が不十分なことに起因する検出や分析の精度低下を防止することができる。 In this example, an opening portion S15 is provided between the first mixing vessel 114f and the second mixing vessel 114h, and an opening portion S17 is provided between the second mixing vessel 114h and the measurement vessel 114i. Therefore, in the first mixing tank 114f and the second mixing tank 114h, after the target mixing and reaction have sufficiently progressed, the opening portions S15 and S17 are opened, and the intermediate preparation liquid and the measurement liquid are respectively supplied to the second mixing tank. 114h and the measuring tank 114i. Therefore, it is possible to prevent a decrease in detection and analysis accuracy due to insufficient mixing and reaction.

　さらに、この例では、第１試薬槽１１４ｅと第１混合槽１１４ｆとの間、第２試薬槽１１４ｇと第２混合槽１１４ｈとの間にも開通部Ｓ１４、Ｓ１６が設けられているため、所望の時点でこれらを開通させて、あらかじめ第１試薬槽１１４ｅおよび第２試薬槽１１４ｇにそれぞれ封入されている第１試薬および第２試薬を第１混合槽１１４ｆや第２混合槽１１４ｈに流入させることができる。仮に開通部Ｓ１４、Ｓ１６が設けられていない場合には、液体流路装置１１０の保管時などに、第１試薬および第２試薬が下流側に流れ始めてしまうおそれがある。
　また、この例の液体流路装置１１０の開通部Ｓ１１～Ｓ１７および閉止部Ｔ１１は、シンプルな構成であり、低コストで形成できる。そのため、この液体流路装置１１０は使い捨てタイプとすることができる。また、開通および閉止の操作も簡便な押圧操作のみで、操作性にも優れる。 Further, in this example, since the opening portions S14 and S16 are provided between the first reagent tank 114e and the first mixing tank 114f and between the second reagent tank 114g and the second mixing tank 114h, it is desirable. These are opened at the point of time, and the first reagent and the second reagent previously sealed in the first reagent tank 114e and the second reagent tank 114g, respectively, are allowed to flow into the first mixing tank 114f and the second mixing tank 114h. Can do. If the opening portions S14 and S16 are not provided, the first reagent and the second reagent may start to flow downstream when the liquid channel device 110 is stored.
Further, the opening portions S11 to S17 and the closing portion T11 of the liquid channel device 110 of this example have a simple configuration and can be formed at low cost. Therefore, the liquid channel device 110 can be a disposable type. In addition, the opening and closing operations can be performed only by a simple pressing operation, and the operability is excellent.

　このような液体流路装置１１０は、基板１１１に、液体流路１１２と液槽と封止材料供給槽１１６と供給路１１８とを形成する第１工程と、形成された液体流路１１２の所定の位置に、栓体１１５を形成する第２工程と、基板１１１において液体流路１１２などが形成された側の流路形成面１１２ａに蓋板１１３を積層する第３工程とを備えた方法により製造できる。
　尚、開通状態から閉止状態にできる液体流路装置の場合では、第２工程は上記の工程と異なり、形成された封止材料供給槽１１６に封止材料を充填する。 Such a liquid flow path device 110 includes a first step of forming a liquid flow path 112, a liquid tank, a sealing material supply tank 116, and a supply path 118 on a substrate 111, and a predetermined liquid flow path 112 formed. A second step of forming the plug body 115 at a position of 3 and a third step of laminating the cover plate 113 on the flow path forming surface 112a on the side of the substrate 111 where the liquid flow path 112 and the like are formed. Can be manufactured.
In the case of the liquid channel device that can be changed from the open state to the closed state, the second step is different from the above step, and the formed sealing material supply tank 116 is filled with the sealing material.

　以下、液体流路装置１１０の製造工程を模式的に示す図２０をさらに参照して、液体流路装置１１０の製造工程について説明する。
　第１工程では、まず、基板１１１の内層１１１ｃを構成するシート１１１ｃ’の巻回物（ロール）１２０と、中間層１１１ｂを構成するシート１１１ｂ’の巻回物１２１と、外層１１１ａを構成するシート１１１ａ’の巻回物１２２とを用意する。
　ついで、内層１１１ｃを構成するシート１１１ｃ’の巻回物１２０から、シート１１１ｃ’を連続的に供給して、打抜機１２３ａにより、液体流路１１２に対応する箇所を線状に打ち抜くとともに、計量槽１１４ｃなどの各液槽の上部に相当する部分を孔状に打ち抜く。また、この液体流路装置１１０は、液体流路１１２の一部から分岐して形成された供給路１１８と封止材料供給槽１１６とを備えているため、このシート１１１ｃ’において、供給路１１８と封止材料供給槽１１６に対応する箇所も、これらに対応する形状に打ち抜く。
　一方、中間層１１１ｂを構成するシート１１１ｂ’の巻回物１２１から、シート１１１ｂ’を連続的に供給して、打抜機１２３ｂにより、計量槽１１４ｃなどの各液槽の下部に対応する箇所を孔状に打ち抜く。 Hereinafter, the manufacturing process of the liquid flow path device 110 will be described with reference to FIG. 20 schematically showing the manufacturing process of the liquid flow path device 110.
In the first step, first, a roll (roll) 120 of a sheet 111c ′ constituting the inner layer 111c of the substrate 111, a roll 121 of the sheet 111b ′ constituting the intermediate layer 111b, and a sheet constituting the outer layer 111a. A wound material 111a ′ is prepared.
Subsequently, the sheet 111c ′ is continuously supplied from the wound material 120 of the sheet 111c ′ constituting the inner layer 111c, and a portion corresponding to the liquid flow path 112 is punched out linearly by a punching machine 123a. A portion corresponding to the upper portion of each liquid tank such as 114c is punched out into a hole shape. Further, the liquid flow path device 110 includes a supply path 118 formed by branching from a part of the liquid flow path 112 and a sealing material supply tank 116. Therefore, in the sheet 111c ′, the supply path 118 is provided. The portions corresponding to the sealing material supply tank 116 are also punched into shapes corresponding thereto.
On the other hand, the sheet 111b ′ is continuously supplied from the wound material 121 of the sheet 111b ′ constituting the intermediate layer 111b, and a portion corresponding to the lower part of each liquid tank such as the measuring tank 114c is opened by the punching machine 123b. Punch into a shape.

　ついで、外層１１１ａを構成するシート１１１ａ’ の巻回物１２２からシート１１１ａ’を連続的に供給する。そして、各シート１１１ａ’、１１１ｂ’、１１１ｃ’を順次積層することにより、基板１１１を製造する。
　ここで各シート１１１ａ’、１１１ｂ’、１１１ｃ’は、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、各シート１１１ａ’、１１１ｂ’、１１１ｃ’の材質によっては、熱融着などにより貼り合わされてもよい。さらに、あらかじめ粘着剤や接着剤が塗布されたシートなどを用いてもよい。 Next, the sheet 111a ′ is continuously supplied from the wound material 122 of the sheet 111a ′ constituting the outer layer 111a. And the board | substrate 111 is manufactured by laminating | stacking each sheet | seat 111a ', 111b', 111c 'sequentially.
Here, each of the sheets 111a ′, 111b ′, and 111c ′ is preferably bonded by an adhesive supplied from an unillustrated adhesive supply device, but depending on the material of each of the sheets 111a ′, 111b ′, and 111c ′. May be bonded together by heat fusion or the like. Furthermore, you may use the sheet | seat etc. which apply | coated the adhesive or the adhesive agent beforehand.

　このように第１工程として、各巻回物１２０、１２１、１２２から各シート１１１ａ’、１１１ｂ’、１１１ｃ’を供給し、シート１１１ｂ’、１１１ｃ’についてはそれぞれ所定の形状に打ち抜き、その後、これら各シート１１１ａ’、１１１ｂ’、１１１ｃ’を順次積層し、接着する工程を採用すると、液体流路１１２および液槽が形成された多数の基板１１１を連続的に生産することができる。このような方法は、一枚の平板からなる各基板に対して液槽や液体流路を例えばフォトリソグラフィ、切削加工などで形成する方法、液槽や液体流路の形成された基板を射出成形などで成形する方法などにくらべて、製造コストが低く、簡便で、大量生産も可能となり、工業的に好適である。
　なお、シート１１１ｂ’、１１１ｃ’を所定の形状に打ち抜き、液体流路１１２、液槽などを形成する方法は低コストで生産性にも優れるが、それ以外の方法（レーザ加工、ナイフなどを用いた切り抜き加工、熱加工など。）で、シート１１１ｂ’、１１１ｃ’を所定の形状に開口させて、液体流路１１２、液槽などを形成してもよい。 As described above, as the first step, the sheets 111a ′, 111b ′, and 111c ′ are supplied from the rolls 120, 121, and 122, and the sheets 111b ′ and 111c ′ are each punched into a predetermined shape. By adopting a process of sequentially laminating and adhering sheets 111a ′, 111b ′, and 111c ′, it is possible to continuously produce a large number of substrates 111 in which the liquid channel 112 and the liquid tank are formed. Such a method is a method in which a liquid tank or a liquid channel is formed on each substrate made of a single flat plate by, for example, photolithography, cutting, or the like. Compared with the method of forming by, etc., the manufacturing cost is low, it is simple, mass production is possible, and it is industrially suitable.
The method of punching the sheets 111b ′ and 111c ′ into a predetermined shape to form the liquid flow path 112 and the liquid tank is low in cost and excellent in productivity, but other methods (laser processing, knife, etc. are used). The sheet 111b ′, 111c ′ may be opened in a predetermined shape by cutting, thermal processing, etc., to form the liquid channel 112, the liquid tank, and the like.

　また、この例では、液体流路装置１１０の基板１１１として、外層１１１ａ、中間層１１１ｂ、内層１１１ｃの３層から構成されたものを示しているが、外層１１１ａと内層１１１ｃとの２層から構成されてもよい。その場合には、内層１１１ｃをなすシート１１１ｃ’に、液体流路１１２、液槽、供給路１１８、封止材料供給槽１１６を形成する。この場合、形成される液体流路１１２と液槽の深さは同じとなる。
　封止材料供給槽１１６は内層１１ｃをなすシート１１１ｃ’に形成されているが、特に、開通状態から閉止状態にできる液体流路装置の場合では、封止材料供給槽の上部を内層１１１ｃをなすシート１１１ｃ’に形成し、下部を中間層１１１ｂをなすシート１１１ｂ’に形成して、封止材料供給槽を液槽と同じ深さになるように形成してもよい。 Further, in this example, the substrate 111 of the liquid channel device 110 is configured by three layers of the outer layer 111a, the intermediate layer 111b, and the inner layer 111c, but is configured by two layers of the outer layer 111a and the inner layer 111c. May be. In that case, the liquid flow path 112, the liquid tank, the supply path 118, and the sealing material supply tank 116 are formed in the sheet 111c ′ forming the inner layer 111c. In this case, the formed liquid flow path 112 and the depth of the liquid tank are the same.
The sealing material supply tank 116 is formed on the sheet 111c ′ forming the inner layer 11c. In particular, in the case of a liquid channel device that can be closed from the open state, the upper part of the sealing material supply tank forms the inner layer 111c. The sealing material supply tank may be formed to have the same depth as the liquid tank by forming the sheet 111c ′ and forming the lower part of the sheet 111b ′ forming the intermediate layer 111b.

　基板１１１の外層１１１ａ、中間層１１１ｂ、内層１１１ｃを構成する各シート１１１ａ’、１１１ｂ’、１１１ｃ’の材質としては、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂、エポキシ樹脂、フェノール樹脂、ＡＢＳ樹脂、ポリプロピレン樹脂、繊維強化プラスチックなどの樹脂が挙げられる。これらのなかでも、透明であって、液体流路１２を流通する液体の様子を目視することができる点では、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂が好ましい。
　なお、本実施形態では、基板１１１として樹脂の巻回物を使用し、その際の液体流路装置１１０の好適な製造方法について説明しているため、シート材料としては、このように樹脂を例示している。しかしながら、製造方法には特に制限はなく、例えば、液体流路装置を安定に支持することが要求される場合などには、基板にガラスなどの樹脂以外の透明材料を使用し、これに切削加工などの方法を適用して液体流路、液槽などを形成することも可能である。 The materials of the sheets 111a ′, 111b ′, 111c ′ constituting the outer layer 111a, the intermediate layer 111b, and the inner layer 111c of the substrate 111 are styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, polyester resin, epoxy Examples thereof include resins such as resins, phenol resins, ABS resins, polypropylene resins, and fiber reinforced plastics. Among these, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin are preferable because they are transparent and the state of the liquid flowing through the liquid flow path 12 can be visually observed.
In this embodiment, since a wound product of resin is used as the substrate 111 and a suitable manufacturing method of the liquid flow path device 110 at that time is described, the resin is exemplified as the sheet material in this way. is doing. However, there is no particular limitation on the manufacturing method. For example, when it is required to stably support the liquid flow path device, a transparent material other than a resin such as glass is used for the substrate, and this is subjected to a cutting process. It is also possible to form a liquid channel, a liquid tank, and the like by applying the above method.

　各シート１１１ａ’、１１１ｂ’、１１１ｃ’の厚さは、適宜設定できるが、図示例の液体流路装置１１０の場合、内層１１１ｃをなすシート１１１ｃ’の厚さは、形成される液体流路１１２の深さに相当し、内層１１１ｃをなすシート１１１ｃ’と中間層１１１ｂをなすシート１１１ｂ’の厚さの和は液槽の総深さに相当する。よって、液槽や液体流路１１２に求められる深さを考慮して、これらシート１１１ｂ’およびシート１１１ｃ’の厚みを決定する。
　具体的には、シート１１１ｂ’の厚さは２５～５００μｍが好ましく、シート１１１ｃ’の厚さは、１０～３００μｍが好ましい。
　また、シート１１１ａ’の厚さは、この例の場合（蓋板１１３が押圧される例の場合）、５０μｍ以上が好ましく、より好ましくは１００～１０００μｍとすると、液体流路装置１０の支持層として十分に作用する。
　液体流路１１２の幅、各液槽および封止材料供給槽１１６の容積、形状などには特に制限はなく、適宜設定できる。例えば、液体流路１１２の幅としては、好ましくは２５～２０００μｍ、より好ましくは５００～２０００μｍ、液槽の容積としては、好ましくは５０～５００００μｌ、より好ましくは１００～１０００μｌである。
　ただし、廃液槽１１４ｄなどについては、特に好適な容積があるわけではなく、各液槽の機能に応じて自由に設計できる。
尚、特に開通状態から閉止状態にできる液体流路装置の場合では、内層１１ｃをなすシート１１ｃ’の厚さは、形成される封止材料供給槽１６および供給路１８の深さにも相当する。　 The thickness of each of the sheets 111a ′, 111b ′, and 111c ′ can be set as appropriate, but in the case of the liquid channel device 110 shown in the drawing, the thickness of the sheet 111c ′ that forms the inner layer 111c is the liquid channel 112 that is formed. The sum of the thicknesses of the sheet 111c ′ forming the inner layer 111c and the sheet 111b ′ forming the intermediate layer 111b corresponds to the total depth of the liquid tank. Therefore, the thickness of the sheet 111b ′ and the sheet 111c ′ is determined in consideration of the depth required for the liquid tank and the liquid channel 112.
Specifically, the thickness of the sheet 111b ′ is preferably 25 to 500 μm, and the thickness of the sheet 111c ′ is preferably 10 to 300 μm.
Further, in this example (in the case where the cover plate 113 is pressed), the thickness of the sheet 111a ′ is preferably 50 μm or more, and more preferably 100 to 1000 μm as the support layer of the liquid flow path device 10. Works well.
There are no particular limitations on the width of the liquid flow path 112, the volume and shape of each liquid tank and the sealing material supply tank 116, and can be set as appropriate. For example, the width of the liquid channel 112 is preferably 25 to 2000 μm, more preferably 500 to 2000 μm, and the volume of the liquid tank is preferably 50 to 50000 μl, more preferably 100 to 1000 μl.
However, the waste liquid tank 114d and the like do not have a particularly suitable volume, and can be freely designed according to the function of each liquid tank.
In particular, in the case of a liquid channel device that can be changed from an open state to a closed state, the thickness of the sheet 11c ′ forming the inner layer 11c corresponds to the depth of the sealing material supply tank 16 and the supply path 18 to be formed. .

　ついで、閉止状態から開通状態にできる液体流路装置の場合では、上述した第１工程で基板１１１に形成された液体流路１１２の一部、すなわち各開通部Ｓ１１～Ｓ１７を設ける各位置に、栓体１１５を形成する第２工程を行う。
　第２工程では、連続的に供給される基板１１１の液体流路１１２における所定位置に、栓体１１５を形成するための栓体形成材料を印刷機（例えばスクリーン印刷機など。）、ディスペンサ、コータ（ロールコータ、ナイフコータなど）などの塗布装置１２４ａで塗布する方法により、栓体１１５を形成する。 Next, in the case of the liquid flow path device that can be opened from the closed state, a part of the liquid flow path 112 formed on the substrate 111 in the first step, that is, each position where the respective opening portions S11 to S17 are provided, A second step of forming the plug body 115 is performed.
In the second step, a plug forming material for forming the plug 115 is formed at a predetermined position in the liquid flow path 112 of the substrate 111 that is continuously supplied by a printing machine (for example, a screen printing machine), a dispenser, and a coater. The stopper 115 is formed by a coating method using a coating device 124a such as a roll coater or a knife coater.

　また、開通状態から閉止状態にできる液体流路装置の場合では、第２工程において、第１工程で基板１１１に形成された液体流路１１２の一部、すなわち各開通部Ｓ１１～Ｓ１７を設ける各位置に、栓体１１５を形成するとともに、第１工程で基板１１１に形成された封止材料供給槽１１６に、封止材料１１７を充填する。
　栓体１１５の形成は、連続的に供給される基板１１１の液体流路１１２における所定位置に、栓体１１５を形成するための栓体形成材料を印刷機、ディスペンサ、コータ（ロールコータ、ナイフコータなど）などの塗布装置１２４ａで塗布する方法により行う。 In the case of the liquid channel device that can be changed from the open state to the closed state, in the second step, a part of the liquid channel 112 formed on the substrate 111 in the first step, that is, each of the opening portions S11 to S17 is provided. The stopper 115 is formed at the position, and the sealing material 117 is filled into the sealing material supply tank 116 formed on the substrate 111 in the first step.
The plug body 115 is formed by printing a plug body forming material for forming the plug body 115 at a predetermined position in the liquid flow path 112 of the substrate 111 supplied continuously by a printing machine, a dispenser, a coater (roll coater, knife coater, etc.). ) Or the like.

　栓体形成材料としては、液体流路１１２を流通する液体と相互作用がなく、閉止状態では確実に液体を閉止し、押圧により塑性変形するものであれば制限はないが、例えば、樹脂成分と、可塑成分と、フィラーと、溶剤とを含有する樹脂組成物を使用する。そして、塗布装置１２４ａとしてディスペンサを使用する場合には、樹脂組成物の粘度は３０～５００ｄＰａ・ｓが好ましく、スクリーン印刷機などを使用する場合には、樹脂組成物の粘度は５０～５００ｄＰａ・ｓが好ましい。
　樹脂成分としては、封止能力およびその安定性、不溶出性、塗布性（印刷性、ディスペンス性など。）などの点から、好ましくはガラス転移温度が－１０℃以下で、質量平均分子量が３０万以下の樹脂が好適に使用される。樹脂の種類としては、例えば、エポキシ樹脂、ポリエステル樹脂、塩素系樹脂、アクリル系樹脂、フタル酸などのエステル系樹脂、などが挙げられ、１種以上の樹脂を使用できる。
　可塑成分としては、ガラス転移温度が３０℃以下の可塑剤が好適に使用される。可塑剤の種類としては、例えば、ハードレジン系樹脂、エポキシ樹脂、ポリエステル樹脂などの低融点を持つ熱可塑性樹脂、などが挙げられ、１種以上の可塑剤を使用できる。 The plug-forming material is not limited as long as it does not interact with the liquid flowing through the liquid flow path 112 and can reliably close the liquid in the closed state and can be plastically deformed by pressing. A resin composition containing a plastic component, a filler, and a solvent is used. When a dispenser is used as the coating device 124a, the viscosity of the resin composition is preferably 30 to 500 dPa · s. When a screen printer or the like is used, the viscosity of the resin composition is 50 to 500 dPa · s. Is preferred.
The resin component preferably has a glass transition temperature of −10 ° C. or less and a mass average molecular weight of 30 from the viewpoint of sealing ability and its stability, non-eluting property, coating property (printability, dispensing property, etc.) and the like. Ten thousand or less resins are preferably used. Examples of the resin include epoxy resins, polyester resins, chlorine resins, acrylic resins, ester resins such as phthalic acid, and the like, and one or more resins can be used.
As the plastic component, a plasticizer having a glass transition temperature of 30 ° C. or less is preferably used. Examples of the plasticizer include thermoplastic resins having a low melting point such as hard resin resins, epoxy resins, and polyester resins. One or more plasticizers can be used.

　フィラーは、栓体形成材料の粘度、栓体形成性を調整するために配合されるものであって、例えば、沈降性硫酸バリウム、タルク、針状酸化ケイ素、中空ビーズなどが挙げられ、１種以上を使用できる。
　これらのうち特に中空ビーズ（ガラス製、樹脂製など。）が配合された樹脂組成物から形成された栓体は、押圧された場合に塑性変形するだけでなく、中空ビーズが割れて破壊されることにより、その分だけ嵩が減る。そのため、このように中空ビーズを含有する栓体によれば、押圧されて押し潰された際に、より扁平に塑性変形し、開通状態において、液体がより流れやすい状態にすることができる。
　溶剤は、栓体形成材料の粘度を調整するために配合されるものであって、適当な有機溶媒が使用される。なお、栓体形成材料が、溶剤を含まなくても印刷などでの塗布が可能なものであれば、溶剤を含まなくてもよく、できれば溶剤を含まないものが好ましい。 The filler is blended to adjust the viscosity of the plug-forming material and the plug-forming property, and examples thereof include precipitated barium sulfate, talc, acicular silicon oxide, and hollow beads. The above can be used.
Of these, plugs formed from resin compositions containing hollow beads (made of glass, resin, etc.) are not only plastically deformed when pressed, but are also broken and broken. As a result, the bulk is reduced accordingly. Therefore, according to the plug body containing hollow beads as described above, when pressed and crushed, the plug body is more flatly plastically deformed, so that the liquid can flow more easily in the open state.
A solvent is mix | blended in order to adjust the viscosity of a plug forming material, Comprising: A suitable organic solvent is used. In addition, as long as the plug body forming material can be applied by printing or the like without containing a solvent, it may not contain a solvent, and preferably contains no solvent.

　このように栓体形成材料を印刷法、ディスペンサ法、コータ法などで塗布する方法によれば、連続的に効率よく栓体１１５を所定の位置に形成できる。 Thus, according to the method of applying the plug body forming material by the printing method, the dispenser method, the coater method, etc., the plug body 115 can be continuously and efficiently formed at a predetermined position.

　また、この第２工程では、閉止部Ｔ１１を構成する封止材料供給槽１１６に、ペースト状の封止材料１１７を塗布装置１２４ｂにより充填する。この際にも、封止材料１１７を上述の印刷法、ディスペンス法、コータ法などにより塗布する方法によれば、連続的に効率よく封止材料１１７を所定の位置に充填できる。
　ペースト状の封止材料１１７としては、液体流路１１２を流通する液体と相互作用がなく、押し出されて液体流路１１２を閉塞できるものであればよいが、例えば、樹脂成分と、可塑成分と、フィラーとを含有し、例えばその粘度が３０～５００ｄＰａ・ｓである樹脂組成物が好適に使用される。また、最終的には、その伸び率が５００％以上となる樹脂組成物が好ましい。
　樹脂成分としては、塗布性（印刷性、ディスペンス性など。）、流動性、封止能力およびその安定性などの点から、好ましくはガラス転移温度が－４０℃以下で、質量平均分子量が５万以下の樹脂が好適に使用される。樹脂の種類としては、例えば、エポキシ樹脂、ポリエステル樹脂、塩素系樹脂、アクリル系樹脂、フタル酸などのエステル系樹脂、などが挙げられ、１種以上の樹脂を使用できる。
　可塑成分としては、ガラス転移温度が３０℃以下の可塑剤が好適に使用される。可塑剤の種類としては、例えば、ハードレジン系樹脂、エポキシ樹脂、ポリエステル樹脂などの低融点を持つ熱可塑性樹脂、などが挙げられ、１種以上の可塑剤を使用できる。
　フィラーは、封止材料１１７の粘度を調整するとともに、封止材料１１７が液体流路１１２に押し出された際に、液体流路１１２を閉塞しやすい形状にするために配合されるものであって、繊維片、体質顔料、チキソトロピック性付与剤などが使用でき、例えば、アエロジル（商品名、日本アエロジル社製）などのフュームドシリカ、沈降性硫酸バリウム、タルクが好適に使用される。 In this second step, the sealing material supply tank 116 constituting the closing portion T11 is filled with the paste-like sealing material 117 by the coating device 124b. Also in this case, according to the method of applying the sealing material 117 by the above-described printing method, dispensing method, coater method, etc., the sealing material 117 can be continuously and efficiently filled into a predetermined position.
The paste-like sealing material 117 may be any material that does not interact with the liquid flowing through the liquid flow path 112 and can be pushed out to close the liquid flow path 112. For example, a resin component, a plastic component, and the like For example, a resin composition containing a filler and having a viscosity of 30 to 500 dPa · s is preferably used. Moreover, finally, a resin composition having an elongation percentage of 500% or more is preferable.
The resin component preferably has a glass transition temperature of −40 ° C. or less and a mass average molecular weight of 50,000 from the viewpoints of coating properties (printability, dispensing properties, etc.), fluidity, sealing ability and stability. The following resins are preferably used. Examples of the resin include epoxy resins, polyester resins, chlorine resins, acrylic resins, ester resins such as phthalic acid, and the like, and one or more resins can be used.
As the plastic component, a plasticizer having a glass transition temperature of 30 ° C. or less is preferably used. Examples of the plasticizer include thermoplastic resins having a low melting point such as hard resin resins, epoxy resins, and polyester resins. One or more plasticizers can be used.
The filler is blended to adjust the viscosity of the sealing material 117 and to make the liquid channel 112 easy to close when the sealing material 117 is pushed out to the liquid channel 112. Fiber pieces, extender pigments, thixotropic imparting agents, and the like can be used. For example, fumed silica such as Aerosil (trade name, manufactured by Nippon Aerosil Co., Ltd.), precipitated barium sulfate, and talc are preferably used.

　このようにして、栓体形成材料と封止材料１１７とをそれぞれ所定の位置に塗布した後、栓体形成材料および封止材料１１７の組成などによって、必要に応じて、これらを加熱乾燥する工程、硬化する工程などの図示略の各工程を行う。 Thus, after apply | coating the plug body forming material and the sealing material 117 in a predetermined position, respectively, the process of heat-drying these as needed by the composition of the plug body forming material and the sealing material 117, etc. Then, each step (not shown) such as a curing step is performed.

　その後、第３工程において、基板１１１の流路形成面１１２ａに蓋板１１３を構成するシート１１３’を積層し、接着する。この場合、蓋板１１３を構成するシート１１３’をその巻回物１２５から連続的に供給することが好ましい。また、ここで基板１１１と、シート１１３’とは、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、これらの材質によっては、熱融着などにより貼り合わされてもよい。これにより、複数の液体流路装置１１０が連続的に連なった連続体を製造することができる。 Thereafter, in the third step, a sheet 113 ′ constituting the cover plate 113 is laminated on the flow path forming surface 112 a of the substrate 111 and bonded. In this case, it is preferable to continuously supply the sheet 113 ′ constituting the cover plate 113 from the wound material 125. Here, the substrate 111 and the sheet 113 ′ are preferably bonded by an adhesive supplied from an adhesive supply device (not shown). However, depending on these materials, the substrates 111 and the sheet 113 ′ are bonded together by heat fusion or the like. May be. Thereby, the continuous body with which the several liquid flow-path apparatus 110 continued continuously can be manufactured.

　こうして製造された液体流路装置１１０の連続体は、図２０に示すように巻き取られて、巻回物１２６の状態とされてもよいし、折り畳まれた状態にされてもよい。また、１枚ずつ切り離された枚葉タイプとされてもよい。巻回物１２６の状態、折り畳まれた状態とされる場合には、各液体流路装置１１０間にミシン目、凹条などのラインを形成する工程を行ってもよい。これにより、各液体流路装置１１０間で屈曲されやすくなり、液体流路装置１１０の連続体を折り畳みやすくすることができる。また、枚葉タイプに切り離しやすくすることもできる。 The continuum of the liquid channel device 110 manufactured in this way may be wound up as shown in FIG. 20 to be in the state of a wound product 126 or in a folded state. Moreover, you may be set as the single wafer type cut | disconnected one sheet at a time. When the wound material 126 is in a folded state or a folded state, a step of forming a line such as a perforation or a concave line between the liquid channel devices 110 may be performed. Thereby, it becomes easy to bend between each liquid flow-path apparatus 110, and can make it easy to fold the continuous body of the liquid flow-path apparatus 110. FIG. Moreover, it can be made easy to separate into a single wafer type.

　なお、蓋板１１３は、図１７Ａの矢印Ａで示すように押圧され荷重が加えられた場合には撓み、その後に荷重が取り去られた場合には、その復元力により元の状態に復元するものである。そのようなものであれば、蓋板１１３の材質、厚さには特に制限はなく、材質としては、例えば、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂、エポキシ樹脂、フェノール樹脂、ＡＢＳ樹脂、ポリプロピレン樹脂、繊維強化プラスチックなどの樹脂が挙げられる。これらのなかでも、透明であって、液体流路１１２を流通する液体の様子を目視することができる点では、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂が好ましい。厚さとしては、閉止状態から開通状態にできる液体流路装置の場合では、１５～３００μｍ程度であれば、蓋板１１３に適した可撓性と復元力とを有するため好ましい。
　開通状態から閉止状態にできる液体流路装置の場合では、蓋板１１３の厚さは、３０～５００μｍ程度が好ましい。 Note that the cover plate 113 bends when it is pressed and a load is applied as indicated by an arrow A in FIG. 17A, and when the load is removed thereafter, the cover plate 113 is restored to its original state by its restoring force. Is. If it is such, there is no restriction | limiting in particular in the material and thickness of the cover board 113, As a material, for example, a styrene resin, an acrylic resin, a polycarbonate resin, a vinyl chloride resin, a PEN resin, a polyester resin, an epoxy resin And resins such as phenol resin, ABS resin, polypropylene resin, and fiber reinforced plastic. Among these, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin are preferable because they are transparent and the state of the liquid flowing through the liquid channel 112 can be visually observed. In the case of a liquid channel device that can be changed from a closed state to an open state, the thickness is preferably about 15 to 300 μm because it has flexibility and restoring force suitable for the lid plate 113.
In the case of the liquid channel device that can be changed from the open state to the closed state, the thickness of the lid plate 113 is preferably about 30 to 500 μm.

　また、第３工程において、蓋板１１３を基板１１１の流路形成面１１２ａに積層、接着する前には、蓋板１１３において栓体１１５と接する部分１１３ａに、シリコーン成分などを含有する離型剤を塗布するなどして、あらかじめ剥離処理を施しておくことが好ましい。このように剥離処理を施しておくと、矢印Ａで示す荷重を取り去った際（図１７Ｂ）に、蓋板１１３と栓体１１５とを速やかに離間させることができ、容易に開通状態にすることができる。一方、栓体１１５と接する部分の液体流路１１２の底部１１２ｂには、接着剤を塗布するなどして、あらかじめ接着処理をしておくことが好ましい。このようにしておくと、この部分には栓体１１５が確実に接着するため、上述の剥離処理と相まってより円滑に、図１７Ｂで示すように栓体１１５を蓋板１１３の側から離間させることができる。 Further, in the third step, before the lid plate 113 is laminated and bonded to the flow path forming surface 112a of the substrate 111, a release agent containing a silicone component or the like in the portion 113a in contact with the plug body 115 in the lid plate 113. It is preferable to perform a peeling treatment in advance, for example, by coating. When the peeling process is performed in this manner, when the load indicated by the arrow A is removed (FIG. 17B), the lid plate 113 and the plug body 115 can be quickly separated and easily opened. Can do. On the other hand, it is preferable that an adhesive is applied in advance to the bottom 112b of the liquid flow path 112 at a portion in contact with the plug body 115, for example. If this is done, the plug body 115 is securely bonded to this portion, so that the plug body 115 is more smoothly separated from the lid plate 113 side as shown in FIG. Can do.

　なお、以上の説明では、図１７Ａおよび図１７Ｂに示すように、開通部Ｓ１１～Ｓ１７を構成する栓体１１５として、蓋板１１３の外側から押圧する操作により塑性変形して、液体流路１１２を開通状態にするものについて例示した。しかしながら、栓体１１５が配置されている部分の液体流路１１２の底部１１２ｂを外側から押圧する操作により、塑性変形する形態としてもよい。その場合には、基板１１１を構成する外層１１１ａと中間層１１１ｂは、押圧され荷重が加えられた場合には撓み、その後に荷重が取り去られた場合には、その復元力により元の状態に復元するように構成されることが必要である。この場合（底部１２ｂを押圧する例の場合）、シート１１１ａ’の厚さを好ましくは１０～３００μｍ、より好ましくは１５～２００μｍとすることが好適である。
　また、その場合には、蓋板１１３において栓体１１５と接する部分１１３ａに接着処理をし、液体流路１１２の底部１１２ｂにおいて栓体１１５と接する部分に剥離処理をしておくことが好ましい。
　同様に、以上の説明では、図１９Ａおよび図１９Ｂに示すように、封止材料供給槽１１６に対応する部分の蓋板１１３を外側から押圧する操作により封止材料１１７が押し出されて、液体流路１１２を閉塞する形態について例示した。しかしながら、封止材料供給槽１１６の底部１１６ａを外側から押圧する操作により、封止材料１１７が押し出される形態としてもよい。その場合にも、基板１１１を構成する外層１１１ａと中間層１１１ｂは、押圧され荷重が加えられた場合には撓むように構成されることが必要である。 In the above description, as shown in FIGS. 17A and 17B, the plug body 115 constituting the opening portions S11 to S17 is plastically deformed by an operation of pressing from the outside of the cover plate 113, so that the liquid channel 112 is formed. It illustrated about what was made into an open state. However, it may be configured to be plastically deformed by an operation of pressing the bottom 112b of the liquid flow path 112 where the plug 115 is disposed from the outside. In that case, the outer layer 111a and the intermediate layer 111b constituting the substrate 111 bend when pressed and a load is applied, and return to the original state by the restoring force when the load is removed thereafter. It is necessary to be configured to restore. In this case (in the example of pressing the bottom 12b), the thickness of the sheet 111a ′ is preferably 10 to 300 μm, more preferably 15 to 200 μm.
In this case, it is preferable that the portion 113 a in contact with the plug body 115 in the lid plate 113 is subjected to an adhesive treatment, and the portion in contact with the plug body 115 in the bottom portion 112 b of the liquid channel 112 is subjected to a peeling process.
Similarly, in the above description, as shown in FIGS. 19A and 19B, the sealing material 117 is pushed out by pressing the lid plate 113 corresponding to the sealing material supply tank 116 from the outside, and the liquid flow An example of closing the path 112 was illustrated. However, the sealing material 117 may be pushed out by pressing the bottom 116a of the sealing material supply tank 116 from the outside. Even in that case, the outer layer 111a and the intermediate layer 111b constituting the substrate 111 need to be configured to bend when pressed and a load is applied.

［第５実施形態］
　図２１は第５実施形態の液体流路装置１１０Ａの一実施形態を概略的に示す平面透視図、図２２は図２１の液体流路装置１１０Ａの一部を拡大した平面透視図、図２３は図２２のＩ－Ｉ’線に沿う断面図である。
　この液体流路装置１１０Ａは、平板からなる四角形の基板１１１Ａと、基板１１１Ａの液体流路１１２が形成された側の流路形成面１１２ａに蓋板１１３Ａが積層されている。その他の構成は、第４実施形態と同じであるので、省略する。　 [Fifth Embodiment]
FIG. 21 is a plan perspective view schematically showing an embodiment of the liquid channel device 110A of the fifth embodiment, FIG. 22 is a plan perspective diagram in which a part of the liquid channel device 110A of FIG. 21 is enlarged, and FIG. FIG. 23 is a cross-sectional view taken along the line II ′ of FIG.
In the liquid channel device 110A, a rectangular substrate 111A made of a flat plate and a cover plate 113A are laminated on a channel forming surface 112a on the side where the liquid channel 112 of the substrate 111A is formed. The other configuration is the same as that of the fourth embodiment, and is omitted.

　この液体流路装置１１０Ａの基板１１１Ａは、図２３に示すように複数層から構成されている。具体的には、外層１１１ａと、その内側に積層した中間層１１１ｂと、その内側に積層した内層１１１ｃの３層から構成されている。
　内層１１１ｃには、液槽（図２３には試料投入槽１１４ａとろ過槽１１４ｂのみ図示）の上部（液槽の蓋板１１３Ａ側の部分。）と、液体流路１１２とが形成されている。
　中間層１１１ｂには、液槽の下部（前記上部以外であって、液槽の底部側の部分。）が形成されている。また、この中間層１１１ｂは、内層１１１ｃ側の面が液体流路１１２の底部１１２ｂを構成している。
　外層１１１ａは、基板１１１Ａの最も外側に配置され、その中間層１１１ｂ側の面が液槽の底部を構成している。
　また、蓋板１１３Ａも複数層から構成されている。具体的には、外層１１３ａとその内側に積層した内層１１３ｂの２層から構成されている。 The substrate 111A of the liquid channel device 110A is composed of a plurality of layers as shown in FIG. Specifically, it is composed of three layers: an outer layer 111a, an intermediate layer 111b stacked on the inner side, and an inner layer 111c stacked on the inner side.
In the inner layer 111c, an upper portion (portion on the lid 113A side of the liquid tank) of the liquid tank (only the sample charging tank 114a and the filtration tank 114b are shown in FIG. 23) and the liquid channel 112 are formed.
In the intermediate layer 111b, a lower part of the liquid tank (a part other than the upper part and on the bottom side of the liquid tank) is formed. Further, in the intermediate layer 111 b, the surface on the inner layer 111 c side forms the bottom portion 112 b of the liquid channel 112.
The outer layer 111a is disposed on the outermost side of the substrate 111A, and the surface on the intermediate layer 111b side forms the bottom of the liquid tank.
The cover plate 113A is also composed of a plurality of layers. Specifically, it is composed of two layers, an outer layer 113a and an inner layer 113b laminated inside.

　そして、この液体流路装置１１０Ａは、第４実施形態と同様に、液体流路１１２の一部を閉止状態から開通状態にする開通部Ｓ１１～Ｓ１７と、開通状態から閉止状態にする閉止部Ｔ１１とを有している。開通部Ｓ１１～Ｓ１７と、閉止部Ｔ１１の構成は、第４実施形態と同じであるので、ここでは省略する。　
　本実施形態では、開通部Ｓ１１～Ｓ１７は、図２３にＳ１１およびＳ１２を例示して説明するように、液体流路１１２内に、液体流路１１２の一部を塞ぐように配置されて液体の流れを封止し、この部分を閉止状態とする樹脂製の栓体１１５を具備している。また、蓋板１１３Ａの内面において、栓体１１５との対向位置には、栓体１１５を収納可能な凹部１５１が形成されている。具体的には、この例では、蓋板１１３Ａの内層１１３ｂが打ち抜きなどで開口され、凹部１５１が形成されている。
　この例の栓体１１５は、図２４Ａに拡大して示すように、閉止状態においては、液体流路１１２の底部１１２ｂと接する部分（底部）が、弱粘着層１１５ａにより、液体流路１１２の底部１１２ｂに固定されている。また、栓体１１５の高さは、液体流路１１２の高さよりも若干高く形成され、その頂部１１５ｂ側が、凹部１５１内に液密にやや嵌入した状態で配置されている。
　一方、凹部１５１において、栓体１１５の頂部１１５ｂとの対向位置には、弱粘着層１１５ａよりも粘着力の大きな強粘着層１１６ａが形成されている。
　そして、栓体１１５は、当該開通部Ｓ１１、Ｓ１２が設けられている部分（栓体１１５や凹部１５１に対応する部分）の蓋板１１３Ａまたは液体流路１１２の底部１１２ｂを外側から押圧する操作により、液体流路１１２から凹部１５１内に移動して、液体流路１１２を閉止状態から開通状態にする。 As in the fourth embodiment, the liquid channel device 110A includes the opening portions S11 to S17 that change a part of the liquid channel 112 from the closed state to the open state, and the closing portion T11 that changes the open state to the closed state. And have. The configurations of the opening portions S11 to S17 and the closing portion T11 are the same as those in the fourth embodiment, and are omitted here.
In the present embodiment, the opening portions S11 to S17 are arranged in the liquid channel 112 so as to block a part of the liquid channel 112, as illustrated by exemplifying S11 and S12 in FIG. There is provided a resin plug 115 that seals the flow and closes this portion. In addition, a recess 151 that can accommodate the plug body 115 is formed on the inner surface of the cover plate 113A at a position facing the plug body 115. Specifically, in this example, the inner layer 113b of the cover plate 113A is opened by punching or the like, and a recess 151 is formed.
As shown in FIG. 24A in an enlarged manner, the plug body 115 in this example has a portion (bottom portion) in contact with the bottom portion 112b of the liquid flow path 112 in the closed state. 112b is fixed. Further, the height of the plug body 115 is formed slightly higher than the height of the liquid channel 112, and the top portion 115 b side is arranged in a state of being slightly liquid-tightly inserted into the recess 151.
On the other hand, in the recess 151, a strong adhesive layer 116a having a larger adhesive force than the weak adhesive layer 115a is formed at a position facing the top 115b of the plug body 115.
Then, the stopper 115 is operated by pressing the lid plate 113A or the bottom 112b of the liquid flow channel 112 from the outside in the part where the opening parts S11 and S12 are provided (the part corresponding to the stopper 115 or the recess 151). Then, the liquid channel 112 is moved from the liquid channel 112 into the recess 151 to change the liquid channel 112 from the closed state to the open state.

　具体的には、図２５Ａおよび図２５Ｂに開通部Ｓ１１を例に挙げて示すように、蓋板１１３Ａを外側から矢印Ａで示すように押圧して荷重を加えると、図２５Ａに示すように蓋板１１３Ａが撓み、凹部１５１の強粘着層１１６ａと栓体１１５の頂部１１５ｂとが接してこれらが粘着する。そして、その後に荷重を取り去ると、図２５Ｂに示すように、蓋板１１３Ａはその復元力により元の状態に復元するが、それにともなって、強粘着層１１６ａの作用により凹部１５１に粘着した栓体１１５は、液体流路１１２の底部１１２ｂから離れ、凹部１５１内に収納された状態になる。その結果、栓体１１５は液体流路１１２の底部１１２ｂから離間し、ここを液体が流通できるようになる。
　このように開通部Ｓ１１～Ｓ１７においては、開通部Ｓ１１～Ｓ１７が設けられている部分の蓋板１１３Ａを外側から押圧して荷重を加えた後、この荷重を取り去る押圧操作によって、栓体１１５が液体流路１１２から凹部１５１内へと移動し、その結果、この部分の液体流路１１２が閉止状態から開通状態となる。 Specifically, as shown in FIG. 25A and FIG. 25B by taking the opening portion S11 as an example, when a load is applied by pressing the cover plate 113A from the outside as indicated by an arrow A, the cover is closed as shown in FIG. 25A. The plate 113A bends, and the strong adhesive layer 116a of the recess 151 and the top 115b of the plug body 115 come into contact with each other and adhere to each other. When the load is subsequently removed, as shown in FIG. 25B, the cover plate 113A is restored to its original state by its restoring force, and accordingly, the plug sticking to the recess 151 by the action of the strong adhesive layer 116a. 115 is separated from the bottom 112 b of the liquid channel 112 and is stored in the recess 151. As a result, the plug 115 is separated from the bottom 112b of the liquid channel 112, and the liquid can flow therethrough.
As described above, in the opening portions S11 to S17, the plug body 115 is moved by pressing the lid plate 113A where the opening portions S11 to S17 are provided from the outside to apply a load and then removing the load. The liquid channel 112 moves from the liquid channel 112 into the recess 151, and as a result, the liquid channel 112 in this portion changes from the closed state to the open state.

　なお、図２５Ａおよび図２５Ｂでは、蓋板１１３Ａを外側から押圧して荷重を加えているが、開通部Ｓ１１が設けられている部分の液体流路１１２の底部１１２ｂを外側から、すなわち、基板１１１Ａの外側から押圧する操作によって、栓体１１５を同様に液体流路１１２から凹部１５１内に移動させてもよい。
　また、液体流路１１２を開通させた後、図２５Ａおよび図２５Ｂでは、弱粘着層１１５ａは液体流路１１２の底部１１２ｂ側に残っているが、栓体１１５側に粘着した状態となっても良い。 In FIGS. 25A and 25B, the load is applied by pressing the cover plate 113A from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the opening portion S11 is provided is from the outside, that is, the substrate 111A. The stopper 115 may be similarly moved from the liquid flow path 112 into the recess 151 by an operation of pressing from the outside.
25A and 25B, the weak adhesive layer 115a remains on the bottom 112b side of the liquid channel 112, but may remain adhered to the plug 115 side after the liquid channel 112 is opened. good.

　一方、この液体流路装置１１０Ａの閉止部Ｔ１１は、図２４Ｂにも示すように、樹脂製の栓体１５０を具備し、この栓体１５０は、蓋板１１３Ａの内面に形成された凹部１５２内に収納されている。具体的には、この例では、蓋板１１３Ａの内層１１３ｂが打ち抜きなどで開口され、凹部１５２が形成され、その内部に、栓体１５０が配置されている。
　この例の栓体１５０は、開通状態においては、弱粘着層１１７ａにより、その頂部１１７ｂが凹部１５２に粘着して、凹部１５２内に収納されている。また、栓体１５０の高さは、液体流路１１２の高さよりも若干高く形成され、後述のように液体流路１１２を閉止状態とした際には、その頂部１１７ｂ側が、凹部１５２内に液密にやや嵌入した状態になるようになっている。
　一方、液体流路１１２の底部１１２ｂにおいて、栓体１５０の底部との対向位置には、強粘着層１１８ａが形成されている。
　そして、栓体１５０は、当該閉止部Ｔ１１が設けられている部分（栓体１５０や凹部１５２に対応する部分）の蓋板１１３Ａまたは液体流路１１２の底部１１２ｂを外側から押圧する操作により、凹部１５２内から液体流路１１２に移動して、液体流路１１２を開通状態から閉止状態にする。 On the other hand, as shown in FIG. 24B, the closing portion T11 of the liquid channel device 110A includes a resin plug 150, and the plug 150 is in a recess 152 formed on the inner surface of the cover plate 113A. It is stored in. Specifically, in this example, the inner layer 113b of the cover plate 113A is opened by punching or the like to form a recess 152, and the plug 150 is disposed therein.
In the opened state, the plug body 150 of this example is housed in the recess 152 with its top 117b sticking to the recess 152 by the weak adhesive layer 117a. Further, the height of the plug 150 is formed slightly higher than the height of the liquid flow path 112. When the liquid flow path 112 is closed as described later, the top portion 117b side of the liquid flow passage 112 is placed in the recess 152. It comes to be in a state where it is closely inserted slightly.
On the other hand, a strong adhesion layer 118 a is formed at a position facing the bottom of the plug 150 at the bottom 112 b of the liquid channel 112.
Then, the plug 150 is recessed by an operation of pressing the lid plate 113A or the bottom 112b of the liquid channel 112 from the outside of the portion where the closing portion T11 is provided (portion corresponding to the plug 150 or the recess 152). The liquid flow path 112 is moved from the inside to the liquid flow path 112, and the liquid flow path 112 is changed from the open state to the closed state.

　具体的には、図２６Ａおよび図２６Ｂに示すように、蓋板１１３Ａを外側から矢印Ｂで示すように押圧して荷重を加えると、図２６Ａに示すように蓋板１１３Ａが撓み、液体流路１１２の底部１１２ｂの強粘着層１１８ａと栓体１５０の底部とが接してこれらが粘着する。そして、その後に荷重を取り去ると、図２６Ｂに示すように、蓋板１１３Ａはその復元力により元の状態に復元するが、その際、栓体１５０は蓋板１１３Ａには同伴されず、強粘着層１１８ａの作用により液体流路１１２の底部１１２ｂに粘着したままの状態となる。このように蓋板１１３Ａが復元しても、栓体１５０はそれに追従せずに液体流路１１２に粘着したままであるため、この栓体１５０により液体流路１１２は閉塞され、液体はこの部分を流通できなくなる。この際、栓体１５０は、上述のとおり、その頂部１１７ｂ側が、凹部１５２内に液密にやや嵌入した状態となる。
　このように閉止部Ｔ１１においては、閉止部Ｔ１１が設けられている部分の蓋板１１３Ａを外側から押圧して荷重を加えた後、この荷重を取り去る押圧操作によって、栓体１５０が凹部１５２内から液体流路１１２へと移動し、その結果、この部分の液体流路１１２が開通状態から閉止状態となる。 Specifically, as shown in FIGS. 26A and 26B, when a load is applied by pressing the cover plate 113A from the outside as indicated by an arrow B, the cover plate 113A is bent as shown in FIG. The strong adhesive layer 118a at the bottom 112b of the 112 and the bottom of the plug 150 come into contact with each other and adhere to each other. Then, when the load is removed thereafter, as shown in FIG. 26B, the cover plate 113A is restored to its original state by its restoring force. At that time, the plug 150 is not accompanied by the cover plate 113A and is strongly adhered. By the action of the layer 118a, the liquid channel 112 remains adhered to the bottom 112b. Even when the cover plate 113A is restored in this way, the plug 150 does not follow it and remains adhered to the liquid flow path 112. Therefore, the liquid flow path 112 is blocked by the plug 150, and the liquid is in this portion. Can not be distributed. At this time, as described above, the plug body 150 is in a state where the top portion 117b side is slightly fitted in the recess 152 in a liquid-tight manner.
As described above, in the closing portion T11, the plug 150 is removed from the inside of the recess 152 by pressing the cover plate 113A of the portion where the closing portion T11 is provided from the outside and applying a load, and then removing the load. The liquid flow path 112 moves to the liquid flow path 112, and as a result, the liquid flow path 112 in this portion changes from the open state to the closed state.

　なお、図２６Ａおよび図２６Ｂでは、蓋板１１３Ａを外側から押圧して荷重を加えているが、閉止部Ｔ１１が設けられている部分の液体流路１１２の底部１１２ｂを外側から、すなわち、基板１１１Ａの外側から押圧する操作によって、栓体１５０を同様に液体流路１１２に移動させてもよい。
　また、液体流路１１２を閉止させた後、図２６Ａおよび図２６Ｂでは、弱粘着層１１７ａは凹部１５２側に残っているが、栓体１５０側に粘着した状態となっても良い。 In FIG. 26A and FIG. 26B, the load is applied by pressing the cover plate 113A from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the closing portion T11 is provided is from the outside, that is, the substrate 111A. The stopper 150 may be similarly moved to the liquid channel 112 by an operation of pressing from the outside.
In addition, after the liquid flow path 112 is closed, the weak adhesive layer 117a remains on the concave portion 152 side in FIGS. 26A and 26B, but may be in a state of sticking to the plug 150 side.

　このような液体流路装置１１０Ａを用いて、測定液を調製する具体的な方法としては、まず、この液体流路装置１１０Ａを試料投入槽１１４ａ側が上方に、測定槽１１４ｉ側が下方に位置するように立てて、液体が重力によって上流側から下流側に流れやすい状態とする。
　ついで、試料をシリンジなどにサンプリングし、このシリンジの針を試料投入槽１１４ａに対応する部分の蓋板１１３Ａに突き刺して、試料投入槽１１４ａに試料を注入する。その後、試料投入槽１１４ａとろ過槽１１４ｂとの間に設けられた開通部Ｓ１１において、上述の押圧操作、すなわち、蓋板１１３Ａまたは液体流路１１２の底部を外側から押圧して荷重を加えた後、取り去る操作を行い、栓体１５を凹部１５１内に移動させ、この部分の液体流路１１２を開通状態とし、試料を重力によりろ過槽１１４ｂまで導入する。
　この際、押圧する操作は、作業者が指で押す手動により行ってもよいし、押圧位置がＸＹ座標としてあらかじめプログラムされている押圧装置などを使用して、所定の位置を押すようにしてもよい。 As a specific method for preparing the measurement liquid using such a liquid channel device 110A, first, the liquid channel device 110A is positioned so that the sample introduction tank 114a side is positioned above and the measurement tank 114i side is positioned below. Therefore, the liquid is likely to flow from the upstream side to the downstream side by gravity.
Next, the sample is sampled in a syringe or the like, and the needle of this syringe is pierced into the cover plate 113A corresponding to the sample loading tank 114a, and the sample is injected into the sample loading tank 114a. Thereafter, in the opening portion S11 provided between the sample introduction tank 114a and the filtration tank 114b, after applying the above-described pressing operation, that is, pressing the bottom of the lid plate 113A or the liquid flow path 112 from the outside. Then, the removal operation is performed, the stopper 15 is moved into the recess 151, the liquid flow path 112 in this portion is opened, and the sample is introduced to the filtration tank 114b by gravity.
At this time, the pressing operation may be performed manually by an operator pressing with a finger, or a predetermined position may be pressed using a pressing device in which the pressing position is programmed in advance as XY coordinates. Good.

　ついで、ろ過槽１１４ｂでろ過処理がなされた後、ろ過槽１１４ｂと計量槽１１４ｃとの間に設けられた開通部Ｓ１２についても、栓体１１５を同様にして凹部１５１内に移動させ、この部分の液体流路１１２を開通状態とし、試料を重力により計量槽１１４ｃに導入する。
　ついで、計量槽１１４ｃにおいて、導入された液体がオーバーフローし始めたことを確認後、ろ過槽１１４ｂと計量槽１１４ｃとの間に設けられた閉止部Ｔ１１について、栓体１５０を凹部１５２から液体流路１１２へと移動させ、この部分の液体流路１１２を閉止状態とする。
　このようにして、計量槽１１４ｃに上流側からの液体がさらに流入するのを停止させてから、計量槽１１４ｃの下流に設けられた開通部Ｓ１３を作動させて、計量槽１１４ｃで計量された試料を第１混合槽１１４ｆに導入する。 Next, after the filtration treatment is performed in the filtration tank 114b, the stopper 115 is also moved into the recess 151 in the same way for the opening portion S12 provided between the filtration tank 114b and the measurement tank 114c. The liquid channel 112 is opened, and the sample is introduced into the measuring tank 114c by gravity.
Next, after confirming that the introduced liquid has started to overflow in the measuring tank 114c, the plug 150 is connected to the liquid channel from the recess 152 in the closing part T11 provided between the filtration tank 114b and the measuring tank 114c. 112, the liquid flow path 112 in this portion is closed.
In this way, after stopping the further flow of the liquid from the upstream side into the measuring tank 114c, the opening portion S13 provided downstream of the measuring tank 114c is operated, and the sample measured in the measuring tank 114c. Is introduced into the first mixing tank 114f.

　こうして計量後の試料を第１混合槽１１４ｆに導入する一方で、第１試薬槽１１４ｅと第１混合槽１１４ｆとの間の開通部Ｓ１４についても、同様に開通状態として、第１試薬を第１混合槽１１４ｆに導入し、試料と第１試薬とを第１混合槽１１４ｆにおいて混合し、中間調製液を調製する。
　ついで、第１混合槽１１４ｆと第２混合槽１１４ｈとの間の開通部Ｓ１５についても、同様に開通状態として、第１混合槽１１４ｆで調製された中間調製液を第２混合槽１１４ｈに導入する。その一方で、第２試薬槽１１４ｇと第２混合槽１１４ｈとの間の開通部Ｓ１６についても、同様に開通状態として、第２試薬を第２混合槽１１４ｈに導入する。そして、中間調製液と第２試薬とを第２混合槽１１４ｈにおいて混合し、測定液を調製する。
　ついで、第２混合槽１１４ｈと測定槽１１４ｉとの間の開通部Ｓ１７についても、同様に開通状態として、第２混合槽１１４ｈで調製された測定液を測定槽１１４ｉに導入する。
　そして、測定槽１１４ｉに測定液を導入した後、この液体流路装置１１０Ａごと検出分析部に供し、目的成分の検出や測定を行う。
　なお、このようにして測定液を調製する過程においては、各液槽に設けられている図示略の連通孔を必要に応じて適宜開閉することにより、液体を流れ易くしたり、流量の正確性を向上させたりする等、液体の流通を制御してもよい。
　このような液体流路装置１１０Ａによれば、上述した第４実施例の液体流路装置１１０と同様に、開通部Ｓ１１～Ｓ１７と、開通状態から閉止状態にする閉止部Ｔ１１、開通部Ｓ１５、Ｓ１４、Ｓ１６とを有するため、液体流路装置１１０と同様な効果を得ることができる。 In this way, while the sample after weighing is introduced into the first mixing tank 114f, the opening portion S14 between the first reagent tank 114e and the first mixing tank 114f is similarly opened, and the first reagent is placed in the first mixing tank 114f. The sample is introduced into the mixing tank 114f, and the sample and the first reagent are mixed in the first mixing tank 114f to prepare an intermediate preparation solution.
Next, also in the opening portion S15 between the first mixing tank 114f and the second mixing tank 114h, the intermediate preparation liquid prepared in the first mixing tank 114f is introduced into the second mixing tank 114h in the same open state. . On the other hand, the opening reagent S16 between the second reagent tank 114g and the second mixing tank 114h is similarly opened, and the second reagent is introduced into the second mixing tank 114h. Then, the intermediate preparation solution and the second reagent are mixed in the second mixing tank 114h to prepare a measurement solution.
Subsequently, the opening portion S17 between the second mixing tank 114h and the measurement tank 114i is similarly opened, and the measurement liquid prepared in the second mixing tank 114h is introduced into the measurement tank 114i.
Then, after introducing the measurement liquid into the measurement tank 114i, the liquid channel device 110A is supplied to the detection analysis unit to detect and measure the target component.
In the process of preparing the measurement liquid in this way, it is possible to facilitate the flow of the liquid or to accurately determine the flow rate by appropriately opening and closing communication holes (not shown) provided in each liquid tank as necessary. The flow of the liquid may be controlled, for example, by improving the flow rate.
According to such a liquid flow path device 110A, as in the liquid flow path device 110 of the fourth embodiment described above, the opening portions S11 to S17, the closing portion T11 that changes the open state to the closed state, the opening portion S15, Since S14 and S16 are included, the same effect as that of the liquid channel device 110 can be obtained.

　この例の液体流路装置１１０Ａ、すなわち、蓋板１１３Ａが２層構造とされ、凹部１５１、１５２が蓋板１１３Ａに形成されている液体流路装置１１０Ａは、次の方法により製造できる。
　すなわち、基板１１１Ａに液体流路１１２と液槽とを形成し、蓋板１１３Ａに凹部１５１、１５２を形成する第１工程と、開通部Ｓ１１～Ｓ１７を構成する栓体１１５を液体流路１１２の一部に形成し、一方、閉止部Ｔ１１を構成する栓体１５０を蓋板１１３Ａの凹部１５２内に形成する第２工程と、基板１１１Ａにおいて液体流路１１２などが形成された側の流路形成面１１２ａに、蓋板１１３Ａにおいて凹部１５１、１５２が形成された側の面（内面）を積層する第３工程とを備えた方法により製造できる。 The liquid channel device 110A in this example, that is, the liquid channel device 110A in which the lid plate 113A has a two-layer structure and the recesses 151 and 152 are formed in the lid plate 113A can be manufactured by the following method.
That is, the liquid flow path 112 and the liquid tank are formed on the substrate 111A, the recesses 151 and 152 are formed on the cover plate 113A, and the plug 115 constituting the opening portions S11 to S17 is connected to the liquid flow path 112. On the other hand, the second step of forming the plug 150 constituting the closing portion T11 in the recess 152 of the lid plate 113A, and the flow path formation on the side where the liquid flow path 112 and the like are formed in the substrate 111A The surface 112a can be manufactured by a method including a third step of laminating the surface (inner surface) on the side where the recesses 151 and 152 are formed in the cover plate 113A.

　以下、液体流路装置１１０Ａの製造工程を模式的に示す図２７をさらに参照して、液体流路装置１１０Ａの製造工程について説明する。
　第１工程では、まず、基板１１１Ａの内層１１１ｃを構成するシート１１１ｃ’の巻回物（ロール）１２０と、中間層１１１ｂを構成するシート１１１ｂ’の巻回物１２１と、外層１１１ａを構成するシート１１１ａ’の巻回物１２２とを用意する。
　ついで、内層１１１ｃを構成するシート１１１ｃ’の巻回物１２０から、シート１１１ｃ’を連続的に供給して、打抜機１２３ａにより、液体流路１１２に対応する箇所を線状に打ち抜くとともに、計量槽１１４ｃなどの各液槽の上部に相当する部分を孔状に打ち抜く。
　一方、中間層１１１ｂを構成するシート１１１ｂ’の巻回物１２１から、シート１１１ｂ’を連続的に供給して、打抜機１２３ｂにより、計量槽１１４ｃなどの各液槽の下部に対応する箇所を孔状に打ち抜く。 Hereinafter, the manufacturing process of the liquid channel device 110A will be described with reference to FIG. 27 schematically showing the manufacturing process of the liquid channel device 110A.
In the first step, first, a roll (roll) 120 of a sheet 111c ′ constituting the inner layer 111c of the substrate 111A, a roll 121 of the sheet 111b ′ constituting the intermediate layer 111b, and a sheet constituting the outer layer 111a. A wound material 111a ′ is prepared.
Subsequently, the sheet 111c ′ is continuously supplied from the wound material 120 of the sheet 111c ′ constituting the inner layer 111c, and a portion corresponding to the liquid flow path 112 is punched out linearly by a punching machine 123a. A portion corresponding to the upper portion of each liquid tank such as 114c is punched out into a hole shape.
On the other hand, the sheet 111b ′ is continuously supplied from the wound material 121 of the sheet 111b ′ constituting the intermediate layer 111b, and a portion corresponding to the lower part of each liquid tank such as the measuring tank 114c is opened by the punching machine 123b. Punch into a shape.

　ついで、外層１１１ａを構成するシート１１１ａ’ の巻回物１２２からシート１１１ａ’を連続的に供給し、各シート１１１ａ’、１１１ｂ’、１１１ｃ’を順次積層することにより、基板１１１Ａを製造する。
　ここで各シート１１１ａ’、１１１ｂ’、１１１ｃ’は、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、各シート１１１ａ’、１１１ｂ’、１１１ｃ’の材質によっては、熱融着などにより貼り合わされてもよい。さらに、あらかじめ粘着剤や接着剤が塗布されたシートなどを用いてもよい。 Next, the sheet 111a ′ is continuously supplied from the wound sheet 122 of the sheet 111a ′ constituting the outer layer 111a, and the sheets 111a ′, 111b ′, and 111c ′ are sequentially stacked to manufacture the substrate 111A.
Here, each of the sheets 111a ′, 111b ′, and 111c ′ is preferably bonded by an adhesive supplied from an unillustrated adhesive supply device, but depending on the material of each of the sheets 111a ′, 111b ′, and 111c ′. May be bonded together by heat fusion or the like. Furthermore, you may use the sheet | seat etc. which apply | coated the adhesive or the adhesive agent beforehand.

　一方、この第１工程では、蓋板１１３Ａの内層１１３ｂを構成するシート１１３ｂ’の巻回物１２５と、蓋板１１３Ａの外層１１３ａを構成するシート１１３ａ’の巻回物１２６も用意し、ついで、内層１１３ｂを構成するシート１１３ｂ’の巻回物１２５から、シート１１３ｂ’を連続的に供給して、打抜機１２３ｃにより、凹部１５１、１５２に対応する箇所を打ち抜く。
　ついで、蓋板１１３Ａの外層１１３ａを構成するシート１１３ａ’ の巻回物１２６からシート１１３ａ’を連続的に供給し、各シート１１３ａ’、１１３ｂ’を積層することにより、蓋板１１３Ａを製造する。
　ここで各シート１１３ａ’、１１３ｂ’は、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、各シート１１３ａ’、１１３ｂ’の材質によっては、熱融着などにより貼り合わされてもよい。さらに、あらかじめ粘着剤や接着剤が塗布されたシートなどを用いてもよい。 On the other hand, in the first step, a wound product 125 of the sheet 113b ′ constituting the inner layer 113b of the cover plate 113A and a wound product 126 of the sheet 113a ′ constituting the outer layer 113a of the cover plate 113A are also prepared. The sheet 113b ′ is continuously supplied from the wound material 125 of the sheet 113b ′ constituting the inner layer 113b, and the portions corresponding to the recesses 151 and 152 are punched by the punching machine 123c.
Subsequently, the sheet 113a ′ is continuously supplied from the wound material 126 of the sheet 113a ′ constituting the outer layer 113a of the cover plate 113A, and the sheets 113a ′ and 113b ′ are laminated to manufacture the cover plate 113A.
Here, the sheets 113a ′ and 113b ′ are preferably bonded by an adhesive supplied from an adhesive supply device (not shown). However, depending on the material of the sheets 113a ′ and 113b ′, heat fusion or the like may be performed. May be pasted together. Furthermore, you may use the sheet | seat etc. which apply | coated the adhesive or the adhesive agent beforehand.

　このように第１工程として、各巻回物１２０、１２１、１２２、１２５、１２６から各シート１１１ａ’、１１１ｂ’、１１１ｃ’、１１３ａ’、１１３ｂ’を供給し、シート１１１ｂ’、１１１ｃ’、１１３ｂ’についてはそれぞれ所定の形状に打ち抜き、その後、各シート１１１ａ’、１１１ｂ’、１１１ｃ’を順次積層、接着する。一方、各シート１１３ａ’、１１３ｂ’も積層、接着する工程を採用すると、液体流路１１２および液槽などが形成された多数の基板１１１Ａと、凹部１５１、１５２が形成された多数の蓋板１１３Ａとをそれぞれ連続的に生産することができる。
　このような方法は、一枚の平板からなる各基板、各蓋板に対して、液槽や液体流路や凹部を例えばフォトリソグラフィ、切削加工などで形成する方法、液槽や液体流路や凹部の形成された基板や蓋板を射出成形などで成形する方法などにくらべて、製造コストが低く、簡便で、大量生産も可能となり、工業的に好適である。
　なお、シート１１１ｂ’、１１１ｃ’、１１３ｂ’を所定の形状に打ち抜き、液体流路１１２、液槽、凹部１５１、１５２などを形成する方法は、低コストで生産性にも優れるが、それ以外の方法（レーザ加工、ナイフなどを用いた切り抜き加工、熱加工など。）で、シート１１１ｂ’、１１１ｃ’、１１３ｂ’を所定の形状に開口させて、液体流路１１２、液槽、凹部１５１、１５２などを形成してもよい。 As described above, as the first step, the sheets 111a ′, 111b ′, 111c ′, 113a ′, and 113b ′ are supplied from the rolls 120, 121, 122, 125, and 126, and the sheets 111b ′, 111c ′, and 113b ′ are supplied. Each of the sheets 111a ′, 111b ′, and 111c ′ is sequentially laminated and bonded to each other. On the other hand, when the process of laminating and bonding the sheets 113a ′ and 113b ′ is adopted, a large number of substrates 111A in which the liquid flow path 112 and the liquid tank are formed, and a large number of cover plates 113A in which the recesses 151 and 152 are formed. Can be produced continuously.
Such a method includes a method of forming a liquid tank, a liquid flow path, and a concave portion, for example, by photolithography, cutting, etc., for each substrate made of a single flat plate and each cover plate, a liquid tank, a liquid flow path, Compared to a method of forming a substrate or a cover plate with a concave portion by injection molding or the like, the manufacturing cost is low, simple and mass production is possible, which is industrially suitable.
The method of punching the sheets 111b ′, 111c ′, 113b ′ into a predetermined shape and forming the liquid channel 112, the liquid tank, the recesses 151, 152, etc. is low in cost and excellent in productivity. The sheet 111b ′, 111c ′, 113b ′ is opened in a predetermined shape by a method (laser processing, cutting using a knife, thermal processing, etc.), and the liquid flow path 112, liquid tank, recesses 151, 152 are opened. Etc. may be formed.

　また、この例では、液体流路装置１１０Ａの基板１１１Ａとして、外層１１１ａ、中間層１１１ｂ、内層１１１ｃの３層から構成されたものを示しているが、外層１１１ａと内層１１１ｃとの２層から構成されてもよい。その場合には、内層１１１ｃをなすシート１１１ｃ’上に、液体流路１１２と液槽を形成する。この場合、形成される液体流路１１２と液槽の深さは同じとなる。 In this example, the substrate 111A of the liquid flow path device 110A is composed of three layers of the outer layer 111a, the intermediate layer 111b, and the inner layer 111c. However, the substrate 111A is composed of two layers of the outer layer 111a and the inner layer 111c. May be. In that case, the liquid flow path 112 and the liquid tank are formed on the sheet 111c ′ forming the inner layer 111c. In this case, the formed liquid flow path 112 and the depth of the liquid tank are the same.

　基板１１１Ａの外層１１１ａ、中間層１１１ｂ、内層１１１ｃを構成する各シート１１１ａ’、１１１ｂ’、１１１ｃ’や、蓋板１１３Ａの外層１１３ａ、内層１１３ｂを構成する各シート１１３ａ’、１１３ｂ’の材質としては、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂、エポキシ樹脂、フェノール樹脂、ＡＢＳ樹脂、ポリプロピレン樹脂、繊維強化プラスチックなどの樹脂が挙げられる。これらのなかでも、透明であって、液体流路１２を流通する液体の様子を目視することができる点では、スチレン樹脂、アクリル樹脂、ポリカーボネート樹脂、塩化ビニル樹脂、ＰＥＮ樹脂、ポリエステル樹脂が好ましい。
　なお、本実施形態では、基板１１１Ａや蓋板１１３Ａの材料として樹脂の巻回物を使用し、その際の液体流路装置１１０Ａの好適な製造方法について説明しているため、シート材料としては、このように樹脂を例示している。しかしながら、製造方法には特に制限はなく、例えば、液体流路装置を安定に支持することが要求される場合などには、基板や蓋板にガラスなどの樹脂以外の透明材料を使用し、これに切削加工などの方法を適用して液体流路、液槽、凹部などを形成することも可能である。 As materials of the sheets 111a ′, 111b ′, 111c ′ constituting the outer layer 111a, the intermediate layer 111b, and the inner layer 111c of the substrate 111A, and the sheets 113a ′, 113b ′ constituting the outer layer 113a and the inner layer 113b of the cover plate 113A, And resins such as styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, polyester resin, epoxy resin, phenol resin, ABS resin, polypropylene resin, and fiber reinforced plastic. Among these, styrene resin, acrylic resin, polycarbonate resin, vinyl chloride resin, PEN resin, and polyester resin are preferable because they are transparent and the state of the liquid flowing through the liquid flow path 12 can be visually observed.
In the present embodiment, a wound material of resin is used as the material of the substrate 111A and the cover plate 113A, and a preferable manufacturing method of the liquid flow path device 110A at that time is described. Therefore, as the sheet material, Thus, resin is illustrated. However, the manufacturing method is not particularly limited. For example, when it is required to stably support the liquid flow path device, a transparent material other than resin such as glass is used for the substrate and the cover plate. It is also possible to form a liquid flow path, a liquid tank, a recess, etc. by applying a method such as cutting to the above.

　各シート１１１ａ’、１１１ｂ’、１１１ｃ’の厚さは、適宜設定することができるが、図示例の液体流路装置１１０Ａの場合、内層１１１ｃをなすシート１１１ｃ’の厚さは、形成される液体流路１１２の深さに相当し、内層１１１ｃをなすシート１１１ｃ’と中間層１１１ｂをなすシート１１１ｂ’の厚さの和は液槽の総深さに相当する。よって、液槽や液体流路１２などに求められる深さを考慮して、これらシート１１１ｂ’およびシート１１１ｃ’の厚みを決定する。
　具体的には、シート１１１ｂ’の厚さは２５～５００μｍが好ましく、シート１１１ｃ’の厚さは、１０～３００μｍが好ましい。
　また、シート１１１ａ’の厚さは、蓋板１１３Ａを押圧して開通部Ｓ１１～Ｓ１７および閉止部Ｔ１１を作動させる場合には、５０μｍ以上が好ましく、より好ましくは１００～１０００μｍである。このような厚さであると、液体流路装置１１０Ａの支持層として十分に作用する。逆に、基板１１１Ａを押圧する場合には、基板１１１Ａは、押圧操作により荷重が加えられた場合には撓み、その後、荷重が取り去られた場合には元に戻る復元力を有する必要がある。その場合には、シート１１１ａ’の厚さは１０～３００μｍが好ましい。
　液体流路１１２の幅、各液槽の容積、形状などには特に制限はなく、適宜設定できる。例えば、液体流路１１２の幅としては、好ましくは２５～２０００μｍ、より好ましくは５００～２０００μｍ、液槽の容積としては、好ましくは５０～５００００μｌ、より好ましくは１００～１０００μｌである。
　ただし、廃液槽１１４ｄなどについては、特に好適な容積があるわけではなく、各液槽の機能に応じて自由に設計できる。 The thickness of each of the sheets 111a ′, 111b ′, and 111c ′ can be set as appropriate. However, in the case of the liquid channel device 110A in the illustrated example, the thickness of the sheet 111c ′ that forms the inner layer 111c is the liquid to be formed. The sum of the thickness of the sheet 111c ′ forming the inner layer 111c and the sheet 111b ′ forming the intermediate layer 111b corresponds to the depth of the flow path 112 and corresponds to the total depth of the liquid tank. Therefore, the thickness of the sheet 111b ′ and the sheet 111c ′ is determined in consideration of the depth required for the liquid tank, the liquid flow path 12, and the like.
Specifically, the thickness of the sheet 111b ′ is preferably 25 to 500 μm, and the thickness of the sheet 111c ′ is preferably 10 to 300 μm.
Further, the thickness of the sheet 111a ′ is preferably 50 μm or more, more preferably 100 to 1000 μm, when the opening portions S11 to S17 and the closing portion T11 are operated by pressing the lid plate 113A. With such a thickness, it sufficiently acts as a support layer for the liquid channel device 110A. On the other hand, when the substrate 111A is pressed, the substrate 111A needs to have a restoring force that bends when a load is applied by the pressing operation and then returns to the original when the load is removed. . In that case, the thickness of the sheet 111a ′ is preferably 10 to 300 μm.
There are no particular limitations on the width of the liquid flow path 112, the volume and shape of each liquid tank, and can be set as appropriate. For example, the width of the liquid channel 112 is preferably 25 to 2000 μm, more preferably 500 to 2000 μm, and the volume of the liquid tank is preferably 50 to 50000 μl, more preferably 100 to 1000 μl.
However, the waste liquid tank 114d and the like do not have a particularly suitable volume, and can be freely designed according to the function of each liquid tank.

　また、各シート１１３ａ’、１１３ｂ’の厚さも、適宜設定することができるが、図示例の液体流路装置１１０Ａの場合、内層１１３ｂをなすシート１１３ｂ’の厚さは、形成される凹部１５１、１５２の深さに相当する。よって、凹部１５１、１５２に求められる深さを考慮して、これらシート１１３ｂ’の厚みを決定する。また、図２５Ａ、図２５Ｂ、および図２６Ａ、図２６Ｂに示したように、蓋板１１３Ａを外側から押圧して、開通部Ｓ１１～Ｓ１７や閉止部Ｔ１１を作動させる場合には、シート１１３ａ’、１１３ｂ’は、押圧操作により荷重が加えられた場合には撓み、その後、荷重が取り去られた場合には元に戻る復元力を有する必要がある。その点も考慮して、これらの厚みを決定する必要がある。
蓋板１１３Ａが押圧される場合には、具体的には、シート１１３ａ’の厚さは１０～３００μｍが好ましく、シート１１３ｂ’の厚さは２５～５００μｍが好ましい。逆に、基板１１１Ａが押圧される場合には、シート１１３ａ’の厚さは５０μｍ以上が好ましい。 Further, the thickness of each sheet 113a ′, 113b ′ can also be set as appropriate. However, in the case of the liquid channel device 110A in the illustrated example, the thickness of the sheet 113b ′ forming the inner layer 113b is set to the recessed portion 151 formed. This corresponds to a depth of 152. Therefore, the thickness of the sheet 113b ′ is determined in consideration of the depth required for the recesses 151 and 152. Further, as shown in FIGS. 25A, 25B, 26A, and 26B, when the cover plate 113A is pressed from the outside to operate the opening portions S11 to S17 and the closing portion T11, the seat 113a ′, 113b ′ needs to have a restoring force that bends when a load is applied by a pressing operation and then returns to the original when the load is removed. Considering this point, it is necessary to determine these thicknesses.
When the cover plate 113A is pressed, specifically, the thickness of the sheet 113a ′ is preferably 10 to 300 μm, and the thickness of the sheet 113b ′ is preferably 25 to 500 μm. Conversely, when the substrate 111A is pressed, the thickness of the sheet 113a ′ is preferably 50 μm or more.

　ついで、第２工程を行う前に、この例の液体流路装置１１０Ａの場合には、栓体１１５、１５０を保持するための弱粘着層１１５ａ、１１７ａと強粘着層１１６ａ、１１８ａとを所定の位置に形成する図示略の粘着層形成工程を行う。弱粘着層１１５ａ、１１７ａと強粘着層１１６ａ、１１８ａの形成は、適切な粘着剤を選定して、それを所定位置に塗布する方法が好適である。 Next, before performing the second step, in the case of the liquid channel device 110A of this example, the weak adhesive layers 115a and 117a and the strong adhesive layers 116a and 118a for holding the stoppers 115 and 150 are bonded to the predetermined steps. An unillustrated adhesive layer forming step to be formed at the position is performed. For forming the weak adhesive layers 115a and 117a and the strong adhesive layers 116a and 118a, a method of selecting an appropriate adhesive and applying it to a predetermined position is suitable.

　強粘着層１１６ａ、１１８ａおよび弱粘着層１１５ａ、１１７ａには、従来公知の粘着剤の中から基板１１１Ａ、蓋板１１３Ａ、栓体１１５、１５０の材質などに応じて適宜選択することができるが、その際、強粘着層１１６ａ、１１８ａを形成する粘着剤の粘着力（粘着強度）は、弱粘着層１１５ａ、１１７ａを形成する粘着剤の粘着力よりも強いことが必要である。強粘着層１１６ａ、１１８ａを形成する粘着剤の粘着力が弱粘着層１１５ａ、１１７ａを形成する粘着剤の粘着力以下であると、開通部Ｓ１１～Ｓ１７や閉止部Ｔ１１において押圧操作をしても、栓体１１５、１５０を液体流路１１２から凹部１５１内に移動させたり、凹部１５２内から液体流路１１２へと移動させたりできない場合や、移動後にそこに保持することができない場合がある。
その場合、液体流路１１２を開通したり閉止したりできなくなる。
　強粘着層１１６ａ、１１８ａを形成する粘着剤の粘着力は、弱粘着層１１５ａ、１１７ａを形成する粘着剤の粘着力よりも０．１Ｎ／ｃｍ以上大きいことが好ましい。さらには、０．１～３０Ｎ／ｃｍの範囲で大きいことが好ましい。強粘着層１１６ａ、１１８ａを形成する粘着剤の粘着力が弱粘着層１１５ａ、１１７ａを形成する粘着剤の粘着力よりも０．１Ｎ／ｃｍ以上大きいと、開通部Ｓ１１～Ｓ１７および閉止部Ｔ１１を確実に作動させることができる。
一方、粘着力の差が３０Ｎ／ｃｍを超えるようにこれらの粘着層を構成することは困難である。
　また、そのうえで、強粘着層１１６ａ、１１８ａの粘着力を１～３０Ｎ／ｃｍの範囲とし、弱粘着層１１５ａ、１１７ａの粘着力を０．０５～５Ｎ／ｃｍの範囲とすることが好ましい。 The strong adhesive layers 116a and 118a and the weak adhesive layers 115a and 117a can be appropriately selected from conventionally known adhesives according to the material of the substrate 111A, the cover plate 113A, the plugs 115 and 150, etc. At that time, the adhesive force (adhesive strength) of the adhesive forming the strong adhesive layers 116a and 118a needs to be stronger than the adhesive force of the adhesive forming the weak adhesive layers 115a and 117a. If the adhesive force of the adhesive forming the strong adhesive layers 116a and 118a is less than or equal to the adhesive force of the adhesive forming the weak adhesive layers 115a and 117a, even if a pressing operation is performed at the opening portions S11 to S17 and the closing portion T11. In some cases, the plugs 115 and 150 cannot be moved from the liquid channel 112 into the recess 151, moved from the recess 152 to the liquid channel 112, or cannot be held there after the movement.
In that case, the liquid channel 112 cannot be opened or closed.
It is preferable that the adhesive strength of the adhesive forming the strong adhesive layers 116a and 118a is 0.1 N / cm or more larger than the adhesive strength of the adhesive forming the weak adhesive layers 115a and 117a. Further, it is preferably large in the range of 0.1 to 30 N / cm. When the adhesive force of the adhesive forming the strong adhesive layers 116a and 118a is 0.1 N / cm or more larger than the adhesive force of the adhesive forming the weak adhesive layers 115a and 117a, the opening portions S11 to S17 and the closing portion T11 are formed. It can be operated reliably.
On the other hand, it is difficult to configure these adhesive layers so that the difference in adhesive strength exceeds 30 N / cm.
In addition, the adhesive strength of the strong adhesive layers 116a and 118a is preferably in the range of 1 to 30 N / cm, and the adhesive strength of the weak adhesive layers 115a and 117a is preferably in the range of 0.05 to 5 N / cm.

　強粘着層１１６ａ、１１８ａおよび弱粘着層１１５ａ、１１７ａに使用する粘着剤としては、例えば、アクリル系、ゴム系、ポリウレタン系、ポリエステル系、シリコン系などが挙げられる。これらのうち、例えば、強粘着層１１６ａ、１１８ａにはアクリル系、ゴム系などを使用し、さらに芯材として、不織布、ポリエステル繊維などを含ませてもよい。弱粘着層１１５ａ、１１７ａには、アクリル系、シリコン系のものを使用することが好ましい。強粘着層１１６ａ、１１８ａと弱粘着層１１５ａ、１１７ａとの粘着力の差を上述の好適な範囲とするためには、各粘着剤を構成する樹脂のガラス転移温度を適宜調整したり、粘着剤に粘着付与剤、硬化剤、芯材などの添加剤を加えたり、その添加量を調整したりする方法が挙げられる。 Examples of the adhesive used for the strong adhesive layers 116a and 118a and the weak adhesive layers 115a and 117a include acrylic, rubber, polyurethane, polyester, and silicon. Among these, for example, the strong adhesive layers 116a and 118a may be made of acrylic or rubber, and may further include a nonwoven fabric or polyester fiber as a core material. As the weak adhesive layers 115a and 117a, it is preferable to use acrylic or silicon-based ones. In order to make the difference in the adhesive strength between the strong adhesive layers 116a and 118a and the weak adhesive layers 115a and 117a within the above-mentioned preferable range, the glass transition temperature of the resin constituting each adhesive is appropriately adjusted, or the adhesive The method of adding additives, such as a tackifier, a hardening | curing agent, and a core material, or adjusting the addition amount, is mentioned.

　なお、ここで「粘着力」とは、ＪＩＳ　Ｚ　０２３７のステンレス板に対する１８０度引きはがし粘着力のことである In addition, "adhesive strength" here means 180 degree peeling adhesive strength against the stainless steel plate of JIS Z 0237.

　ついで、第２工程において、第１工程で基板１１１Ａに形成された液体流路１１２の一部、すなわち、各開通部Ｓ１１～Ｓ１７を設ける各位置に、栓体１１５を形成するとともに、第１工程で蓋板１１３Ａに形成された凹部１５１、１５２のうち、閉止部Ｔ１１に対応する凹部１５２内に、栓体１５０を形成する。
　栓体１１５の形成は、連続的に供給される基板１１１Ａの所定位置に、栓体１１５を形成するための栓体形成材料を印刷機、ディスペンサ、コータ（ロールコータ、ナイフコータなど）などの塗布装置１２４ａで塗布する方法により行う。栓体１５０の形成も、同様の塗布装置１２４ｂを用いて、栓体形成材料を凹部１５２に塗布、充填する方法で行う。 Next, in the second step, a plug body 115 is formed at a part of the liquid flow path 112 formed on the substrate 111A in the first step, that is, at each position where the opening portions S11 to S17 are provided. Thus, the plug 150 is formed in the recess 152 corresponding to the closing portion T11 among the recesses 151 and 152 formed in the cover plate 113A.
The plug body 115 is formed by applying a plug body forming material for forming the plug body 115 to a predetermined position of the substrate 111A that is continuously supplied, such as a printing machine, a dispenser, and a coater (roll coater, knife coater, etc.). This is performed by the coating method 124a. The plug body 150 is also formed by applying and filling the plug body forming material into the recess 152 using the same coating device 124b.

　栓体形成材料としては、例えば、その粘度が３０～６００ｄＰａ・ｓの範囲にある樹脂組成物が好適に使用され、上記粘度範囲にある樹脂組成物が溶剤を含まないものであればより好ましい。
　樹脂組成物に含まれる樹脂成分としては、塗布性（印刷性、ディスペンス性など。）、栓体としての封止能力およびその安定性が良好で、栓体形成材料とした際に上記範囲の粘度となる樹脂であればその種類に制限はない。
　また、樹脂組成物には適当な可塑成分が含まれてもよい。 As the plug forming material, for example, a resin composition having a viscosity in the range of 30 to 600 dPa · s is preferably used, and the resin composition in the above viscosity range is more preferable if it does not contain a solvent.
The resin component contained in the resin composition has good coating properties (printability, dispensing properties, etc.), sealing ability as a plug and stability thereof, and viscosity in the above range when used as a plug forming material. There is no limitation on the type of the resin.
The resin composition may contain an appropriate plastic component.

　樹脂組成物には、栓体形成材料の粘度を調整するためのフィラーが含まれてもよく、例えば、沈降性硫酸バリウム、タルク、針状酸化ケイ素、中空ビーズなどが挙げられ、１種以上を使用できる。
　溶剤は、必要に応じて、栓体形成材料の粘度を調整するために配合されるものであって、適当な有機溶媒が使用される。 The resin composition may contain a filler for adjusting the viscosity of the plug-forming material, and examples thereof include precipitated barium sulfate, talc, acicular silicon oxide, and hollow beads. Can be used.
A solvent is mix | blended in order to adjust the viscosity of a plug body forming material as needed, and a suitable organic solvent is used.

　このように栓体形成材料を印刷法、ディスペンサ法、コータ法などで塗布する方法によれば、連続的に効率よく栓体１１５、１５０を所定の位置に形成できる。 Thus, according to the method in which the plug forming material is applied by a printing method, a dispenser method, a coater method, etc., the plugs 115 and 150 can be continuously and efficiently formed at predetermined positions.

　栓体形成材料をそれぞれ所定の位置に塗布した後、栓体形成材料の組成などによって、必要に応じて、これを加熱乾燥する工程、硬化する工程などの図示略の各工程を行う。 After each plug body forming material is applied to a predetermined position, each step (not shown) such as a heat drying process and a curing process is performed as necessary depending on the composition of the plug body forming material.

　その後、第３工程において、基板１１１Ａの流路形成面１１２ａに、蓋板１１３Ａの凹部１５１、１５２が形成された側の面を積層し、接着する。また、ここで基板１１１Ａと、蓋板１１３Ａとは、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、これらの材質によっては、熱融着などにより貼り合わされてもよい。さらに、あらかじめ粘着剤や接着剤が塗布されたシートなどを用いてもよい。
　これにより、複数の液体流路装置１１０Ａが連続的に連なった連続体を製造することができる。 Thereafter, in the third step, the surface on the side where the recesses 151 and 152 of the cover plate 113A are formed is laminated and bonded to the flow path forming surface 112a of the substrate 111A. Here, the substrate 111A and the cover plate 113A are preferably bonded by an adhesive supplied from an adhesive supply device (not shown). However, depending on these materials, the substrates 111A and the cover plate 113A are bonded together by heat fusion or the like. May be. Furthermore, you may use the sheet | seat etc. which apply | coated the adhesive or the adhesive agent beforehand.
Thereby, a continuous body in which a plurality of liquid flow path devices 110A are continuously connected can be manufactured.

　こうして製造された液体流路装置１１０Ａの連続体は、図２７に示すように巻き取られて、巻回物１２７の状態とされてもよいし、折り畳まれた状態にされてもよい。また、１枚ずつ切り離された枚葉タイプとされてもよい。巻回物２７の状態、折り畳まれた状態とされる場合には、各液体流路装置１１０Ａ間にミシン目、凹条などのラインを形成する工程を行ってもよい。これにより、各液体流路装置１１０Ａ間で屈曲されやすくなり、液体流路装置１１０Ａの連続体を折り畳みやすくすることができる。また、枚葉タイプに切り離しやすくすることもできる。 The continuum of the liquid channel device 110A thus manufactured may be wound up as shown in FIG. 27 to be in the state of a wound object 127 or in a folded state. Moreover, you may be set as the single wafer type cut | disconnected one sheet at a time. When the wound product 27 is in a folded state or a folded state, a process of forming a line such as a perforation or a concave line between the liquid channel devices 110A may be performed. Thereby, it becomes easy to bend between each liquid flow-path apparatus 110A, and can make it easy to fold the continuous body of 110 A of liquid flow-path apparatuses. Moreover, it can be made easy to separate into a single wafer type.

　なお、以上説明した第５実施形態では、各開通部Ｓ１１～Ｓ１７および閉止部Ｔ１１において、栓体１１５、１５０は弱粘着層１１５ａ、１１７ａによりあらかじめ所定位置に保持され、その後、押圧操作により、液体流路１１２から凹部１５１内、あるいは、凹部１５２内から液体流路１１２に移動し、強粘着層１１６ａ、１１８ａにより所定位置に保持される形態とした。
　しかしながら、このように粘着力の差を利用した方法により、栓体１１５、１５０を所定位置に移動させ、そこに保持する形態には限定されない。
　例えば、栓体１１５、１５０の形状、材質、凹部１５１、１５２の形状、凹部１５１、１５２が形成される蓋板１１３Ａの材質などを調整することにより、開通状態において、栓体１１５、１５０が凹部１５１、１５２内に弾性力などにより密に保持されるようにすれば、必ずしも、凹部１５１、１５２内に弱粘着層１１７ａや強粘着層１１６ａは設けられなくてもよい。
　また、閉止状態において、栓体１１５、１５０が液体流路１１２の所定位置に確実に保持され、そこから移動してしまわないように、例えば図２８に示すように、液体流路１１２の底部１１２ｂから凸起して、栓体１１５、１５０を挟持する一対の栓体受け１１９ａ、１１９ｂなどを形成してもよい。栓体受け１１９ａ、１１９ｂを例えば弾性体などから形成してすると、弾性力により栓体１１５、１５０を液体流路１１２の所定位置からズレないように保持することができる。
　さらに、例えば、図２９に開通部Ｓ１１を例示して説明するように、栓体１１５の平面視形状を菱形などとして、その最大幅Ｗ１を液体流路１１２の幅Ｗ２よりも大きく設定するとともに、液体流路１１２の両側壁には、栓体１１５の幅方向の両端部が係止される係止凹部を形成しておく方法などによって、閉止状態において、栓体１１５、１５０の幅方向の両端部が係止凹部に係止され、液体流路１１２の所定位置から液体流路に沿って（図中上下方向）ズレないようにしてもよい。なお、図２９では、平面視形状が菱形の栓体１１５を例示したが、栓体１１５の最大幅Ｗ１が液体流路１１２の幅Ｗ２よりも大きく形成され、液体流路の両側壁に、栓体１１５を係止可能な係止凹部が形成される限り、栓体の平面視形状は菱形に限定されない。
　さらに、弱粘着層１１５ａ、１１７ａ、強粘着層１１６ａ、１１８ａを設けた上で、栓体受け１１９ａ、１１９ｂを設けたり、栓体１１５、１５０の平面視形状を菱形にしたりするなど、栓体１１５、１５０を保持する手段としてこれらを併用してもよい。 In the fifth embodiment described above, in each of the opening portions S11 to S17 and the closing portion T11, the plugs 115 and 150 are held in place by the weak adhesive layers 115a and 117a in advance, and then the liquid is pressed by a pressing operation. The liquid channel 112 is moved from the flow path 112 to the recess 151 or from the recess 152 to the liquid flow path 112 and is held at a predetermined position by the strong adhesion layers 116a and 118a.
However, the present invention is not limited to a mode in which the stoppers 115 and 150 are moved to a predetermined position and held there by a method using the difference in adhesive force.
For example, by adjusting the shape and material of the plugs 115 and 150, the shape of the recesses 151 and 152, the material of the lid plate 113A on which the recesses 151 and 152 are formed, the plugs 115 and 150 are recessed in the open state. As long as it is held tightly by elastic force or the like in 151 and 152, the weak adhesive layer 117a and the strong adhesive layer 116a are not necessarily provided in the recesses 151 and 152.
Further, in the closed state, the bottoms 112b of the liquid flow path 112 are, for example, shown in FIG. 28 so that the plugs 115 and 150 are securely held at predetermined positions of the liquid flow path 112 and do not move from there. A pair of plug receivers 119a and 119b that sandwich the plugs 115 and 150 may be formed. If the plug body receivers 119a and 119b are formed of, for example, an elastic body, the plug bodies 115 and 150 can be held so as not to be displaced from a predetermined position of the liquid channel 112 by an elastic force.
Further, for example, as illustrated in FIG. 29 exemplifying the opening part S11, the planar view shape of the plug body 115 is set to a diamond shape, and the maximum width W1 is set larger than the width W2 of the liquid flow path 112, and In the closed state, both ends of the plugs 115 and 150 in the width direction are formed on both side walls of the liquid channel 112 by, for example, a method of forming locking recesses that lock both ends of the plug 115 in the width direction. The portion may be locked in the locking recess so as not to be displaced from a predetermined position of the liquid channel 112 along the liquid channel (vertical direction in the figure). In FIG. 29, the plug body 115 having a diamond shape in plan view is illustrated, but the maximum width W1 of the plug body 115 is formed larger than the width W2 of the liquid channel 112, and plugs are formed on both side walls of the liquid channel. As long as a locking recess capable of locking the body 115 is formed, the planar view shape of the plug is not limited to a rhombus.
Further, the plug body 115 includes a weak adhesive layer 115a, 117a, and a strong adhesive layer 116a, 118a, a plug body receiver 119a, 119b, and a planar view of the plug bodies 115, 150 having a rhombus shape. , 150 may be used in combination as a means for holding.

［第６実施形態］
　以上説明した第５実施形態の液体流路装置１１０Ａでは、蓋板１１３Ａは外層１１３ａおよび内層１１３ｂの２層から構成され、基板１１１Ａは外層１１１ａ、中間層１１１ｂ、内層１１１ｃの３層から構成され、凹部１５１、１５２は蓋板１１３Ａに形成された形態を例示した。
　以下、本第６実施形態では、図３０～３３に示すように、蓋板１１３は１層で構成され、一方、基板１１１Ｂは外層１４１ａ、外側中間層１４１ｂ、内側中間層１４１ｃ、内層１４１ｄの４層から構成され、凹部１５１、１５２は蓋板１１３ではなく液体流路１１２の底部１１２ｂに形成された形態について例示する。 [Sixth Embodiment]
In the liquid channel device 110A of the fifth embodiment described above, the cover plate 113A is composed of two layers of the outer layer 113a and the inner layer 113b, and the substrate 111A is composed of three layers of the outer layer 111a, the intermediate layer 111b, and the inner layer 111c. The recessed parts 151 and 152 illustrated the form formed in the cover plate 113A.
Hereinafter, in the sixth embodiment, as shown in FIGS. 30 to 33, the cover plate 113 is composed of one layer, while the substrate 111B has four layers of an outer layer 141a, an outer intermediate layer 141b, an inner intermediate layer 141c, and an inner layer 141d. A configuration in which the concave portions 151 and 152 are formed on the bottom portion 112 b of the liquid channel 112 instead of the lid plate 113 is illustrated.

　この例の液体流路装置１１０Ｂは、図３０に示すように、その基板１１１Ｂは、外層１４１ａと、その内側に積層した外側中間層１４１ｂと、その内側に積層した内側中間層１４１ｃと、その内側に積層した内層１４１ｄの４層から構成されている。
　内層１４１ｄには、液槽（図３０には試料投入槽１１４ａとろ過槽１１４ｂのみ図示）の上部（液槽の蓋板１１３側の部分。）と、液体流路１１２とが形成されている。
　内側中間層１４１ｃには、液槽の中間部（前記上部以外の部分のうち、液槽の底部側の部分を除いた中間部分。）と、凹部１５１、１５２とが形成されている。また、この内側中間層１４１ｃは、内層１１１ｄ側の面が液体流路１１２の底部１１２ｂを構成している。
　外側中間層１４１ｂには、液槽の下部（液槽の底部側の部分であり、前記上部および中間部以外の部分。）が形成されている。また、この外側中間層１４１ｂは、内側中間層１４１ｃ側の面が凹部１５１、１５２の底部を構成している。
　外層１４１ａは、基板１１１Ｂの最も外側に配置され、その外側中間層１４１ｂ側の面が液槽の底部を構成している。
　また、蓋板１１３は、１層のみから構成されている。 As shown in FIG. 30, the liquid flow path device 110B in this example includes an outer layer 141a, an outer intermediate layer 141b stacked on the inner side, an inner intermediate layer 141c stacked on the inner side, and an inner side thereof. The inner layer 141d is laminated in four layers.
In the inner layer 141d, an upper portion (portion on the lid plate 113 side of the liquid tank) of the liquid tank (only the sample charging tank 114a and the filtration tank 114b are shown in FIG. 30) and the liquid channel 112 are formed.
In the inner intermediate layer 141c, an intermediate part of the liquid tank (an intermediate part excluding the part on the bottom side of the liquid tank among the parts other than the upper part) and concave parts 151 and 152 are formed. In addition, the inner intermediate layer 141 c has a surface on the inner layer 111 d side that forms the bottom 112 b of the liquid channel 112.
The outer intermediate layer 141b is formed with a lower part of the liquid tank (a part on the bottom side of the liquid tank, and a part other than the upper part and the intermediate part). In the outer intermediate layer 141b, the surface on the inner intermediate layer 141c side forms the bottom of the recesses 151 and 152.
The outer layer 141a is disposed on the outermost side of the substrate 111B, and the surface on the outer intermediate layer 141b side forms the bottom of the liquid tank.
The lid plate 113 is composed of only one layer.

　そして、この液体流路装置１１０Ｂでは、開通部Ｓ１１～Ｓ１７は、図３０にＳ１１およびＳ１２を例示して説明するように、液体流路１１２内に、液体流路１１２の一部を塞ぐように配置されて液体の流れを封止し、この部分を閉止状態とする樹脂製の栓体１１５を具備している。また、液体流路１１２の底部１１２ｂにおいて、この栓体１１５の対向位置には、栓体１１５を収納可能な凹部１５１が形成されている。
　この例の栓体１１５は、図３１Ａに拡大して示すように、閉止状態においては、蓋板１１３と接する部分（頂部）が、弱粘着層１１５ａにより、蓋板１１３の内面に固定されている。また、栓体１１５の高さは、液体流路の高さよりも若干高く形成され、その底部１１５ｃ側が、凹部１５１内に液密にやや嵌入した状態で配置されている。
　そして、栓体１１５は、当該開通部Ｓ１１、Ｓ１２が設けられている部分の蓋板１１３または液体流路１１２の底部１１２ｂを外側から押圧する操作により、液体流路１１２から凹部１５１内に移動して、液体流路１１２を閉止状態から開通状態にする。 In the liquid channel device 110B, the opening portions S11 to S17 are configured so as to block a part of the liquid channel 112 in the liquid channel 112, as described with reference to S11 and S12 in FIG. A resin plug 115 is disposed to seal the flow of the liquid and to close this portion. In addition, a concave portion 151 that can accommodate the plug body 115 is formed at a position facing the plug body 115 at the bottom 112 b of the liquid flow path 112.
As shown in an enlarged view in FIG. 31A, the plug body 115 in this example is fixed to the inner surface of the lid plate 113 by a weak adhesive layer 115a in a closed state (top portion) in contact with the lid plate 113. . Further, the height of the plug 115 is formed slightly higher than the height of the liquid flow path, and the bottom 115c side is arranged in a state of being slightly fitted in the recess 151 in a liquid-tight manner.
Then, the stopper 115 moves from the liquid channel 112 into the recess 151 by an operation of pressing the lid plate 113 or the bottom 112b of the liquid channel 112 where the opening portions S11 and S12 are provided from the outside. Thus, the liquid channel 112 is changed from the closed state to the open state.

　具体的には、図３２Ａおよび図３２Ｂの開通部Ｓ１を例に挙げて示すように、蓋板１１３を外側から矢印Ｃで示すように押圧して荷重を加えると、図３２Ａに示すように蓋板１１３が撓み、凹部１５１の強粘着層１１６ａと栓体１１５の底部１１５ｃとが接し、これらが粘着する。そして、その後に荷重を取り去ると、図３２Ｂに示すように、蓋板１１３はその復元力により元の状態に復元するが、その際、栓体１１５は、強粘着層１１６ａの作用により凹部１５１内に収納された状態で保持される。その結果、蓋板１１３が復元するのに伴って、栓体１１５は蓋板１１３から離間し、ここを液体が流通できるようになる。
　このように開通部Ｓ１１～Ｓ１７においては、開通部Ｓ１１～Ｓ１７が設けられている部分の蓋板１１３を外側から押圧して荷重を加えた後、この荷重を取り去る押圧操作によって、栓体１１５が液体流路１１２から凹部１５１内へと移動し、その結果、この部分の液体流路１１２が閉止状態から開通状態となる。 Specifically, as shown in FIG. 32A and FIG. 32B by taking the opening portion S1 as an example, when a load is applied by pressing the lid plate 113 from the outside as indicated by an arrow C, the lid as shown in FIG. 32A. The plate 113 bends, and the strong adhesive layer 116a of the recess 151 comes into contact with the bottom 115c of the stopper 115, and these stick together. Then, when the load is removed thereafter, as shown in FIG. 32B, the cover plate 113 is restored to its original state by its restoring force. At that time, the plug 115 is placed in the recess 151 by the action of the strong adhesive layer 116a. It is held in a state of being housed in the container. As a result, as the cover plate 113 is restored, the plug 115 is separated from the cover plate 113 so that the liquid can flow therethrough.
As described above, in the opening portions S11 to S17, the lid 115 is pressed by pressing the lid plate 113 of the portion where the opening portions S11 to S17 are provided from the outside, and then the plug 115 is removed by a pressing operation for removing the load. The liquid channel 112 moves from the liquid channel 112 into the recess 151, and as a result, the liquid channel 112 in this portion changes from the closed state to the open state.

　なお、図３２Ａおよび図３２Ｂでは、蓋板１１３を外側から押圧して荷重を加えているが、開通部Ｓ１１が設けられている部分の液体流路１１２の底部１１２ｂを外側から、すなわち、基板１１１Ｂの外側から押圧する操作によって、栓体１１５を液体流路１１２から凹部１５１内に移動させてもよい。 32A and 32B, the load is applied by pressing the cover plate 113 from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the opening portion S11 is provided is from the outside, that is, the substrate 111B. The plug body 115 may be moved from the liquid flow path 112 into the recess 151 by an operation of pressing from the outside.

　一方、この液体流路装置１１０Ｂの閉止部Ｔ１１は、図３１Ｂにも示すように、樹脂製の栓体１５０を具備し、この栓体１５０は、液体流路１１２の底部１１２ｂに形成された凹部１５２内に収納されている。具体的には、この例では、基板１１１Ｂの内側中間層１４１ｃが打ち抜きなどで開口されて凹部１５２が形成され、その内部に、栓体１５０が配置されている。
　この例の栓体１５０は、開通状態においては、その底部が弱粘着層１１７ａにより凹部１５２に粘着して、凹部１５２内に収納されている。また、栓体１５０の高さは、液体流路１１２の高さよりも若干高く形成され、後述のように液体流路１１２を閉止状態とした際には、その底部側が、凹部１５２内にやや嵌入した状態になるようになっている。
　一方、蓋板１１３の内面において、栓体１５０の頂部との対向位置には、強粘着層１１８ａが形成されている。
　そして、栓体１５０は、当該閉止部Ｔ１１が設けられている部分の蓋板１１３または液体流路１１２の底部１１２ｂを外側から押圧する操作により、凹部１５２内から液体流路１１２に移動して、液体流路１１２を開通状態から閉止状態にする。 On the other hand, as shown in FIG. 31B, the closing portion T11 of the liquid channel device 110B includes a resin plug 150, which is a recess formed in the bottom 112b of the liquid channel 112. It is housed in 152. Specifically, in this example, the inner intermediate layer 141c of the substrate 111B is opened by punching or the like to form the recess 152, and the plug 150 is disposed therein.
In the opened state, the plug 150 of this example is housed in the recess 152 with its bottom adhered to the recess 152 by the weak adhesive layer 117a. In addition, the height of the plug 150 is slightly higher than the height of the liquid channel 112. When the liquid channel 112 is closed as described later, the bottom side of the plug 150 is slightly inserted into the recess 152. It is supposed to be in the state.
On the other hand, a strong adhesion layer 118 a is formed on the inner surface of the lid plate 113 at a position facing the top of the plug 150.
Then, the stopper 150 moves from the inside of the recess 152 to the liquid flow path 112 by an operation of pressing the lid plate 113 or the bottom 112b of the liquid flow path 112 in the portion where the closing portion T11 is provided, The liquid channel 112 is changed from the open state to the closed state.

　具体的には、図３３Ａおよび図３３Ｂに示すように、蓋板１３を外側から矢印Ｄで示すように押圧して荷重を加えると、図３３Ａに示すように蓋板１１３が撓み、蓋板１１３の強粘着層１１８ａと栓体１５０の頂部とが接し、これらが粘着する。そして、その後に荷重を取り去ると、図３３Ｂに示すように、蓋板１１３はその復元力により元の状態に復元するが、その際、栓体１５０は、強粘着層１１８ａの作用により蓋板１１３の内側に粘着した状態で保持される。その結果、栓体１５０は、凹部１５２から液体流路１１２へと移動する。こうして、この栓体１５０により液体流路１１２は閉塞され、液体はこの部分を流通できなくなる。
　このように閉止部Ｔ１１においては、閉止部Ｔ１１が設けられている部分の蓋板１１３を外側から押圧して荷重を加えた後、この荷重を取り去る押圧操作によって、栓体１５０が凹部１５２内から液体流路１１２へと移動し、その結果、この部分の液体流路１１２が開通状態から閉止状態となる。 Specifically, as shown in FIGS. 33A and 33B, when a load is applied by pressing the cover plate 13 from the outside as indicated by an arrow D, the cover plate 113 is bent as shown in FIG. The strong adhesion layer 118a and the top of the plug 150 are in contact with each other, and these adhere to each other. Then, when the load is removed thereafter, as shown in FIG. 33B, the cover plate 113 is restored to its original state by its restoring force. At this time, the plug 150 is covered by the strong adhesive layer 118a. It is held in an adhesive state inside. As a result, the plug 150 moves from the recess 152 to the liquid channel 112. Thus, the liquid flow path 112 is blocked by the plug 150, and the liquid cannot flow through this portion.
As described above, in the closing portion T11, the stopper 150 is pushed from the outside by pressing the cover plate 113 of the portion where the closing portion T11 is provided, and then the plug 150 is removed from the recess 152 by a pressing operation to remove the load. The liquid flow path 112 moves to the liquid flow path 112, and as a result, the liquid flow path 112 in this portion changes from the open state to the closed state.

　なお、図３３Ａおよび図３３Ｂでは、蓋板１１３を外側から押圧して荷重を加えているが、閉止部Ｔ１１が設けられている部分の液体流路１１２の底部１１２ｂを外側から、すなわち、基板１１１Ｂの外側から押圧する操作によって、栓体１５０を液体流路１１２に移動させるようにしてもよい。 33A and 33B, the load is applied by pressing the cover plate 113 from the outside, but the bottom 112b of the liquid flow path 112 in the portion where the closing portion T11 is provided is from the outside, that is, the substrate 111B. The stopper 150 may be moved to the liquid flow path 112 by an operation of pressing from the outside of the liquid.

　この例の液体流路装置１１０Ｂは、基板１１１Ｂに液体流路１１２と液槽と凹部１５１、１５２とを形成する第１工程と、開通部Ｓ１１～Ｓ１７を構成する栓体１１５を、当該開通部Ｓ１１～Ｓ１７が設けられる部分の蓋板１１３の内面に形成し、一方、閉止部Ｔ１１を構成する栓体１５０を閉止部Ｔ１１に対応する凹部１５２内に形成する第２工程と、基板１１１Ｂにおいて液体流路１１２などが形成された側の流路形成面１１２ａに、蓋板１１３の栓体１１５が形成された側の面を積層する第３工程とを備えた方法により製造できる。 In this example, the liquid flow path device 110B includes a first step of forming the liquid flow path 112, the liquid tank, and the recesses 151 and 152 in the substrate 111B, and the plug body 115 constituting the opening portions S11 to S17. A second step of forming the plug 150 constituting the closing portion T11 in the recess 152 corresponding to the closing portion T11, and a second step of forming the plug 150 constituting the closing portion T11 on the inner surface of the cover plate 113 where S11 to S17 are provided; And a third step of laminating the surface of the lid plate 113 on which the plug body 115 is formed on the flow channel forming surface 112a on the side where the flow channel 112 and the like are formed.

　第１工程では、図３４に示すように、まず、基板１１１Ｂの内層１４１ｄを構成するシート１４１ｄ’の巻回物１２８と、内側中間層１４１ｃを構成するシート１４１ｃ’の巻回物１２９と、外側中間層１４１ｂを構成するシート１４１ｂ’の巻回物１３０と、外層１４１ａを構成するシート１４１ａ’の巻回物１３１とを用意する。
　ついで、内層１４１ｄを構成するシート１４１ｄ’の巻回物１２８から、シート１４１ｄ’を連続的に供給して、打抜機１２３ａにより、液体流路１１２に対応する箇所を線状に打ち抜くとともに、計量槽１１４ｃなどの各液槽の上部に相当する部分を孔状に打ち抜く。
　一方、内側中間層１４１ｃを構成するシート１４１ｃ’の巻回物１２９から、シート１４１ｃ’を連続的に供給して、打抜機１２３ｂにより、計量槽１１４ｃなどの各液槽の中間部に対応する部分を孔状に打ち抜き、また、凹部１５１、１５２に対応する部分も打ち抜く。
　また、外側中間層１４１ｂを構成するシート１４１ｂ’の巻回物１３０から、シート１４１ｂ’を連続的に供給して、打抜機１２３ｃにより、計量槽１１４ｃなどの各液槽の下部に対応する部分を孔状に打ち抜く。 In the first step, as shown in FIG. 34, first, the wound material 128 of the sheet 141d ′ constituting the inner layer 141d of the substrate 111B, the wound material 129 of the sheet 141c ′ constituting the inner intermediate layer 141c, and the outer side A wound product 130 of the sheet 141b ′ constituting the intermediate layer 141b and a wound product 131 of the sheet 141a ′ constituting the outer layer 141a are prepared.
Next, the sheet 141d ′ is continuously supplied from the wound material 128 of the sheet 141d ′ constituting the inner layer 141d, and a portion corresponding to the liquid flow path 112 is punched out linearly by a punching machine 123a. A portion corresponding to the upper portion of each liquid tank such as 114c is punched into a hole.
On the other hand, the sheet 141c ′ is continuously supplied from the wound material 129 of the sheet 141c ′ constituting the inner intermediate layer 141c, and the portion corresponding to the intermediate portion of each liquid tank such as the measuring tank 114c by the punching machine 123b. Are punched into holes, and the portions corresponding to the recesses 151 and 152 are also punched.
Further, the sheet 141b ′ is continuously supplied from the wound material 130 of the sheet 141b ′ constituting the outer intermediate layer 141b, and a portion corresponding to the lower part of each liquid tank such as the measuring tank 114c is formed by the punching machine 123c. Punched into a hole.

　ついで、外層１４１ａを構成するシート１４１ａ’ の巻回物１３１からシート１４１ａ’を連続的に供給し、各シート１４１ａ’、１４１ｂ’、１４１ｃ’１４１ｄ’を順次積層することにより、基板１１１Ｂを製造する。
　ここで各シート１４１ａ’、１４１ｂ’、１４１ｃ’、１４１ｄ’は、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、各シート１４１ａ’、１４１ｂ’、１４１ｃ’、１４１ｄ’の材質によっては、熱融着などにより貼り合わされてもよい。さらに、あらかじめ粘着剤や接着剤が塗布されたシートなどを用いてもよい。 Subsequently, the sheet 141a ′ is continuously supplied from the wound material 131 of the sheet 141a ′ constituting the outer layer 141a, and the respective sheets 141a ′, 141b ′, 141c ′ 141d ′ are sequentially laminated to manufacture the substrate 111B. .
Here, each sheet 141a ′, 141b ′, 141c ′, 141d ′ is preferably bonded by an adhesive supplied from an adhesive supply device (not shown), but each sheet 141a ′, 141b ′, 141c ′. , 141d ′ may be bonded together by heat fusion or the like. Furthermore, you may use the sheet | seat etc. which apply | coated the adhesive or the adhesive agent beforehand.

　ついで、第２工程を行う前に、第５実施形態の場合と同様にして、栓体１１５、１５０を保持するための弱粘着層１１５ａ、１１７ａと強粘着層１１６ａ、１１８ａとを所定の位置に形成する図示略の粘着層形成工程を行う。 Next, before performing the second step, as in the fifth embodiment, the weak adhesive layers 115a and 117a and the strong adhesive layers 116a and 118a for holding the stoppers 115 and 150 are placed at predetermined positions. An unillustrated adhesive layer forming step to be formed is performed.

　ついで、第２工程において、蓋板１１３を構成するシート１１３’の巻回物１３２から、シート１１３’を連続的に供給して、各開通部Ｓ１１～Ｓ１７の位置に対応するように、蓋板１１３の内面に栓体１１５を形成する。一方、第１工程で液体流路１１２の底部１１２ｂに形成された凹部１５１、１５２のうち、閉止部Ｔ１１を構成する凹部１５２内に、栓体１５０を形成する。
　栓体１１５の形成は、第５実施形態において説明したように、栓体１１５を形成するための栓体形成材料を印刷機、ディスペンサ、コータ（ロールコータ、ナイフコータなど）などの塗布装置１２４ａで塗布する方法により好適に行える。また、栓体１５０の形成も、連続的に供給される基板１１１Ｂに形成された凹部１５２内に、栓体形成材料を同様の塗布装置１２４ｂで塗布し、充填する方法により好適に行える。
　また、栓体形成材料としても、第５実施形態で例示したものを同様に好適に使用できる。 Next, in the second step, the sheet 113 ′ is continuously supplied from the wound material 132 of the sheet 113 ′ constituting the cover plate 113 so as to correspond to the positions of the opening portions S11 to S17. A plug 115 is formed on the inner surface of 113. On the other hand, the plug 150 is formed in the recess 152 constituting the closing portion T11 among the recesses 151 and 152 formed in the bottom 112b of the liquid channel 112 in the first step.
As described in the fifth embodiment, the plug body 115 is formed by applying a plug body forming material for forming the plug body 115 with a coating device 124a such as a printing machine, a dispenser, or a coater (roll coater, knife coater, etc.). It can be suitably performed by the method. Further, the plug body 150 can be preferably formed by a method in which the plug body forming material is applied and filled in the concave portion 152 formed in the substrate 111B that is continuously supplied by the same coating device 124b.
In addition, as the plug forming material, those exemplified in the fifth embodiment can be suitably used as well.

　その後、第３工程において、基板１１１Ｂの流路形成面１１２ａに、蓋板１１３の内面、すなわち栓体１１５が形成された側の面を積層し、接着する。また、ここで基板１１１Ｂと、蓋板１１３とは、図示略の接着剤供給装置から供給される接着剤により、接着されることが好ましいが、これらの材質によっては、熱融着などにより貼り合わされてもよい。さらに、あらかじめ粘着剤や接着剤が塗布されたシートなどを用いてもよい。これにより、複数の液体流路装置１１０Ｂが連続的に連なった連続体を製造することができる。 Thereafter, in the third step, the inner surface of the cover plate 113, that is, the surface on which the plug 115 is formed is laminated on the flow path forming surface 112a of the substrate 111B and bonded. Here, the substrate 111B and the cover plate 113 are preferably bonded by an adhesive supplied from an adhesive supply device (not shown). However, depending on these materials, the substrates 111B and the cover plate 113 are bonded together by heat fusion or the like. May be. Furthermore, you may use the sheet | seat etc. which apply | coated the adhesive or the adhesive agent beforehand. Thereby, a continuous body in which a plurality of liquid flow path devices 110B are continuously connected can be manufactured.

　こうして製造された液体流路装置１１０Ｂの連続体は、第５実施形態の場合と同様に、巻き取られて、巻回物１３３の状態とされてもよいし、折り畳まれた状態にされてもよい。
また、１枚ずつ切り離された枚葉タイプとされてもよい。また、各液体流路装置１１０Ｂ間にミシン目、凹条などのラインを形成する工程を行ってもよい。 As in the case of the fifth embodiment, the continuous body of the liquid flow path device 110B thus manufactured may be wound into a wound product 133 or a folded state. Good.
Moreover, you may be set as the single wafer type cut | disconnected one sheet at a time. Moreover, you may perform the process of forming lines, such as a perforation and a groove, between each liquid flow-path apparatus 110B.

　なお、この例の場合にも、第５実施形態の場合と同様に、栓体１１５、１５０の形状、材質、凹部１５１、１５２の形状、凹部１５１、１５２が形成される基板１１１Ｂの材質などを調整することにより、開通状態において、栓体１１５、１５０が凹部１５１、１５２内に弾性力などにより密に保持されるようにすれば、必ずしも、凹部１５１、１５２内に弱粘着層１１７ａや強粘着層１１６ａは設けられなくてもよい。
　また、第５実施形態の場合と同様に、栓体１１５、１５０を挟持する一対の栓体受け１１９ａ、１１９ｂなどを形成してもよい。
　また、閉止状態において、栓体１１５、１５０の幅方向の両端部が係止凹部に係止され、液体流路１１２の所定位置から液体流路に沿って（図中上下方向）ズレないようにしてもよい。
　さらに、これらの手法を組み合わせて併用してもよい。 Also in this example, as in the case of the fifth embodiment, the shape and material of the plugs 115 and 150, the shape of the recesses 151 and 152, the material of the substrate 111B on which the recesses 151 and 152 are formed, and the like. If the plugs 115 and 150 are tightly held in the recesses 151 and 152 by an elastic force or the like in the opened state by adjusting, the weak adhesive layer 117a or the strong adhesive layer 117a or the strong adhesive layer is not necessarily in the recesses 151 and 152. The layer 116a may not be provided.
Further, as in the case of the fifth embodiment, a pair of plug receivers 119a and 119b that sandwich the plugs 115 and 150 may be formed.
In the closed state, both end portions in the width direction of the plugs 115 and 150 are locked by the locking recesses so that the liquid channel 112 is not displaced from a predetermined position along the liquid channel (vertical direction in the figure). May be.
Furthermore, these methods may be used in combination.

　以上、第４～第６実施形態で例示した液体流路装置１１０、１１０Ａ、１１０Ｂでは、基板１１１Ａ、１１１Ｂの片面のみに液体流路１１２が形成されているが、基板１１１Ａ、１１１Ｂの両面に液体流路１１２が形成されてもよい。
　また、各液槽に必要に応じて設けられる、開閉可能な連通孔の形態には制限はなく、蓋板１１３、１１３Ａに形成された連通孔に、嵌め込み式のキャップを抜き差しすることで、連通孔を開通、閉止できる形態などでもよい。また、液体流路１１２に設けられる開通部Ｓ１１～Ｓ１７および閉止部Ｔ１１と同様の構成の開通部と閉止部とを連通孔に設けてもよい。
　また、各液槽には、必要に応じて、送液部を設けてもよい。送液部の具体的な形態としては、その液槽に対応する部分の蓋板１１３またはその液槽の底部を外側から押圧する操作により、その液槽の内容積が小さくなり、その液槽内の液体が吐出されて、下流側に送液されるような形態が挙げられる。このような送液部を設けた液槽には、液体の上流側への逆流を防止する堰板などの逆流防止部を設けることが好適である。 As described above, in the liquid flow path devices 110, 110A, and 110B exemplified in the fourth to sixth embodiments, the liquid flow path 112 is formed only on one surface of the substrates 111A and 111B, but the liquid flow path 112 is formed on both surfaces of the substrates 111A and 111B. A channel 112 may be formed.
Moreover, there is no restriction | limiting in the form of the communication hole which can be opened and closed provided as needed in each liquid tank, and it can communicate by inserting / removing a fitting type cap to the communication hole formed in the cover plates 113 and 113A. The form etc. which can open and close a hole may be sufficient. Further, an opening part and a closing part having the same configuration as the opening parts S11 to S17 and the closing part T11 provided in the liquid channel 112 may be provided in the communication hole.
Moreover, you may provide a liquid feeding part in each liquid tank as needed. As a specific form of the liquid feeding part, the inner volume of the liquid tank is reduced by the operation of pressing the lid plate 113 corresponding to the liquid tank or the bottom of the liquid tank from the outside, The liquid is discharged and sent to the downstream side. The liquid tank provided with such a liquid feeding part is preferably provided with a backflow prevention part such as a weir plate for preventing the backflow of the liquid to the upstream side.

　以上の例においては、液体を流通させるために、重力の作用を利用した形態について示したが、遠心力の作用を利用してもよい。例えば、この液体流路装置１１０、１１０Ａ、１１０Ｂを試料投入槽１１４ａ側が回転中心側に位置し、測定槽１１４ｉ側が回転の外周側に位置するように遠心装置にセットして液体流路装置１１０、１１０Ａ、１１０Ｂを回転させ、液体流路１１２の上流側から下流側に遠心力が働き、その結果、液体が流れるようにしてもよい。
　また、このように液体流路装置１１０、１１０Ａ、１１０Ｂを回転させ、遠心力を利用して液体を流通させる際には、開通部Ｓ１１～Ｓ１７や閉止部Ｔ１１を作動させるための各押圧操作には、圧接ディスクを用いることもできる。圧接ディスクは、液体流路装置１１０、１１０Ａ、１１０Ｂの蓋板１１３、１１３Ａの表面を回転中心側から回転の外周側に向かって、回転の半径方向に移動しながら、所定位置を押圧するものである。 In the above example, in order to circulate the liquid, the form using the action of gravity is shown, but the action of centrifugal force may be used. For example, the liquid channel devices 110, 110A, and 110B are set in the centrifuge so that the sample introduction tank 114a side is located on the rotation center side and the measurement tank 114i side is located on the outer periphery side of the rotation. 110A and 110B may be rotated so that centrifugal force acts from the upstream side to the downstream side of the liquid channel 112, and as a result, the liquid may flow.
Further, when the liquid flow path devices 110, 110A, and 110B are rotated and the liquid is circulated using the centrifugal force in this way, each pressing operation for operating the opening portions S11 to S17 and the closing portion T11 is performed. A pressure contact disk can also be used. The pressure contact disk presses a predetermined position while moving in the radial direction of the rotation from the rotation center side to the rotation outer peripheral side on the surface of the cover plate 113, 113A of the liquid flow path device 110, 110A, 110B. is there.

　また、重力や遠心力を利用して液体を移動させる以外に、液体流路１１２、液槽の一部、またはこれらの両方を加熱して液体流路１１２や液槽内の空気を膨張させたり、液体流路１１２の一部に酸素吸収剤（酸化しやすい鉄粉など）を封入しておき、液体流路１１２内の酸素を吸収することで液体流路１１２内を減圧にしたりして、液体を移動させ、流通させる方法などを併用してもよい。 In addition to moving the liquid using gravity or centrifugal force, the liquid flow path 112, a part of the liquid tank, or both of them are heated to expand the air in the liquid flow path 112 or the liquid tank. In addition, an oxygen absorbent (such as iron powder that easily oxidizes) is sealed in a part of the liquid channel 112, and the inside of the liquid channel 112 is decompressed by absorbing oxygen in the liquid channel 112, A method of moving and distributing the liquid may be used in combination.

　また、以上の説明では、試料投入槽１１４ａに試料を注入する方法として、シリンジの針を蓋板１１３、１１３Ａに突き刺す方法を例示しているが、例えば、あらかじめ蓋板１１３、１１３Ａに試料注入孔を形成しておき、そこから試料を注入してもよい。その場合、試料注入孔には保護テープを被せておき、シリンジを保護テープに突き刺すことで注入してもよいし、保護テープを剥がして試料注入孔にシリンジを挿入して注入してもよい。 In the above description, as a method of injecting the sample into the sample introduction tank 114a, a method of piercing the needles of the syringe into the cover plates 113 and 113A is exemplified. The sample may be injected from there. In that case, the sample injection hole may be covered with a protective tape and injected by piercing the protective tape into the protective tape, or the protective tape may be peeled off and the syringe inserted into the sample injection hole for injection.

　液体流路装置１１０、１１０Ａ、１１０Ｂを流通させる試料および試薬としては、特に制限はなく、医療分野、環境分野などで従来より採用されている試料と試薬とを適宜組み合わせて使用することができる。
　例えば、医療分野においては、試料として、血液（全血）、血漿、血清、バフィーコート、尿、糞便、唾液、喀痰などの生体由来のもの、ウィルス、細菌、カビ、酵母、動植物の細胞などが挙げられる。また、これらから単離したＤＮＡまたはＲＮＡを用いてもよいし、これらに対して何らかの前処理、希釈などが施されたものを試料としてもよい。
　なお、先に例示した液体流路装置１１０、１１０Ａ、１１０Ｂは、試料投入槽１１４ａの下流に、試料投入槽１１４ａから流通してきた試料に対してろ過処理を施すためのろ過槽１１４ｂを備えている。よって、このような液体流路装置１１０、１１０Ａ、１１０Ｂを用いると、例えば、血液から血球をろ過処理で取り除く場合など、従来はあらかじめ別のろ過装置でろ過処理を行う必要があった試料を予めろ過処理することなく、そのまま液体流路装置１１０、１１０Ａ、１１０Ｂの試料投入槽１４ａに供することができる。 There are no particular limitations on the sample and reagent through which the liquid channel devices 110, 110A, and 110B are circulated, and a sample and a reagent that have been conventionally used in the medical field, the environmental field, and the like can be used in appropriate combination.
For example, in the medical field, samples of blood (whole blood), plasma, serum, buffy coat, urine, feces, saliva, sputum, etc., viruses, bacteria, mold, yeast, animal and plant cells, etc. Can be mentioned. In addition, DNA or RNA isolated from these may be used, and a sample obtained by subjecting them to any pretreatment or dilution may be used.
The liquid channel devices 110, 110A, and 110B exemplified above include a filtration tank 114b for performing a filtration process on the sample flowing from the sample input tank 114a downstream of the sample input tank 114a. . Therefore, when such liquid flow path devices 110, 110A, and 110B are used, for example, in the case where blood cells are removed from blood by filtration, a sample that conventionally had to be previously filtered by another filtration device is previously stored. Without filtration, the sample can be used as it is in the sample introduction tank 14a of the liquid flow path devices 110, 110A, 110B.

　試薬としては、特に制限はなく、目的成分に応じて適宜選択できるが、試料中に存在する抗原を抗原抗体反応を利用して捕捉、分析する場合には、抗原に対する抗体を含有する試薬が好ましい。
　なお、以上の例では、第１試薬槽１１４ｅや第２試薬槽１１４ｇに、例えば抗体が含まれる試薬をあらかじめ封入しておき、これらの試薬と、例えば抗原が含まれる試料とを第１混合槽１１４ｆや第２混合槽１１４ｈで混合することにより、抗原が抗体に捕捉される形態を例示した。しかしながら、抗原を抗体に捕捉させる形態はこのような形態に限定されず、例えば、抗体あるいは抗体を担持させた磁性ビーズを液体流路装置１１０、１１０Ａ、１１０Ｂの液槽または液体流路の途中などに固定しておき、そこを試料が流通することにより、試料中の抗原が抗体に捕捉されるようにしてもよい。ついで、適当な試薬を試料投入槽１１４ａなどからシリンジなどで投入するとともに、必要に応じて上述した送液部も適宜利用するなどして、このように捕捉された抗原を洗浄したり、変性させたり、増殖（濃度上昇）させたり、分離したりして、分析精度を高めることもできる。 The reagent is not particularly limited and may be appropriately selected depending on the target component. However, when an antigen present in a sample is captured and analyzed using an antigen-antibody reaction, a reagent containing an antibody against the antigen is preferable. .
In the above example, a reagent containing, for example, an antibody is sealed in the first reagent tank 114e or the second reagent tank 114g in advance, and these reagents and a sample containing, for example, an antigen are mixed in the first mixing tank. An example in which the antigen is captured by the antibody by mixing in 114f or the second mixing tank 114h is illustrated. However, the form in which the antigen is captured by the antibody is not limited to such a form. For example, the antibody or the magnetic beads carrying the antibody are placed in the liquid tanks of the liquid channel devices 110, 110A, 110B or in the middle of the liquid channel. The antigen in the sample may be captured by the antibody by allowing the sample to flow therethrough. Next, an appropriate reagent is introduced from the sample introduction tank 114a or the like with a syringe or the like, and the above-described liquid feeding unit is appropriately used as necessary to wash or denature the captured antigen. Or can be increased (concentration increase) or separated to increase the analysis accuracy.

　また、液体流路装置１１０、１１０Ａ、１１０Ｂで行う反応としては、抗原抗体反応に限らず、各種化学反応、ＤＮＡを増幅させるＰＣＲ（polymerase chain reaction）、ＤＮＡなどのタンパクを捕捉する反応なども実施できる。複数の反応を組み合わせることもできるし、液体流路装置１１０内では混合処理のみを行うなど、必ずしも何らかの反応を行わなくてもよい。このように液体流路装置１１０、１１０Ａ、１１０Ｂの使用方法には何ら限定はない。 In addition, the reactions performed in the liquid channel devices 110, 110A, and 110B are not limited to antigen-antibody reactions, but include various chemical reactions, PCR (polymerase chain reaction) that amplifies DNA, and reactions that capture proteins such as DNA. it can. A plurality of reactions may be combined, or some reaction may not necessarily be performed, for example, only the mixing process is performed in the liquid channel device 110. Thus, there is no limitation on the method of using the liquid channel devices 110, 110A, 110B.

　また、このような各種反応を促進したり、液体の流れを促進したりするために、液体流路１１２や液槽に対して、各種の処理を行うことができる。例えば、酸、アルカリなどを用いた化学的処理、ラテックス、蛍光物質などを用いた物理的処理、抗原、抗体、ＤＮＡなどを用いた生化学的処理を種々施して、例えば、親水処理、親油処理、撥水処理などの表面処理効果を得ることができる。その他にも、塗料の塗布処理、プラズマ処理、フレーム処置などを実施してもよい。さらに、液体流路１１２には、必要に応じて、邪魔板、攪拌板、突起を設けたり、分水形状を形成したりして、流通する液体が均一な混合状態となるようにしてもよい。また、液体流路１１２や液槽内を必要に応じて加圧しておいたり（加圧処理）、減圧しておいたり（真空処理）してもよい。 Further, in order to promote such various reactions or promote the flow of liquid, various treatments can be performed on the liquid flow path 112 and the liquid tank. For example, various chemical treatments using acids, alkalis, etc., physical treatments using latex, fluorescent substances, etc., biochemical treatments using antigens, antibodies, DNA, etc. Surface treatment effects such as treatment and water repellent treatment can be obtained. In addition, coating treatment, plasma treatment, flame treatment, and the like may be performed. Furthermore, the liquid flow path 112 may be provided with a baffle plate, a stirring plate, a protrusion, or formed with a water diverting shape, if necessary, so that the flowing liquid is in a uniform mixed state. . Further, the inside of the liquid channel 112 and the liquid tank may be pressurized as necessary (pressure treatment) or depressurized (vacuum treatment).

　さらに、着色剤、色素、蛍光剤などを適当な液槽内に投入しておき、そこに到達した試料を着色したり、試料に蛍光を付与したりすることも可能である。
　また、液体流路装置１１０，１１０Ａ、１１０Ｂの蓋板１１３、１１３Ａ、液体流路１１２、液槽、基板１１１、１１１Ａ、１１１Ｂなどの任意の箇所に、必要に応じて、この液体流路装置１１０，１１０Ａ、１１０Ｂを用いて行う操作の内容や手順、液槽の名称（例えば「計量槽」など。）などを直接印刷してもよい。または、操作の内容や手順、液槽の名称などが印刷された表示シールなどを貼着したり、何らかの目印となるマーキングを設けてもよい。さらに、例えば蓋板１１３、１１３Ａの一部のみを透明化するなどして、その箇所が目立つようにしてもよい。 Further, it is possible to put a colorant, a dye, a fluorescent agent, etc. in an appropriate liquid tank, and to color the sample that has reached it or to impart fluorescence to the sample.
Further, the liquid channel device 110, 110A, 110B of the liquid channel device 110, 110A, 110B, such as the lid plate 113, 113A, the liquid channel 112, the liquid tank, the substrate 111, 111A, 111B, etc. , 110A, 110B, the contents and procedure of the operation performed, the name of the liquid tank (for example, “weighing tank”, etc.), etc. may be directly printed. Or you may stick the display sticker etc. which printed the content and procedure of operation, the name of a liquid tank, etc., or may provide the marking used as a certain mark. Further, for example, only a part of the cover plates 113 and 113A may be made transparent so that the portions become conspicuous.

　液体流路装置１１０，１１０Ａ、１１０Ｂで調製された測定液の検出分析部としては、従来公知の光学的手段、電気的手段などを適宜採用することができる。また、その際に、液体流路装置１１０，１１０Ａ、１１０Ｂを必要に応じて加熱したり冷却したりしてもよい。 Conventionally known optical means, electrical means, and the like can be appropriately employed as the detection / analysis unit for the measurement liquid prepared by the liquid flow path devices 110, 110A, and 110B. At that time, the liquid flow path devices 110, 110A, and 110B may be heated or cooled as necessary.

１０Ａ、１０Ｂ　液体流路装置
１１Ａ、１１Ｂ　基板
１１ｅ　外層
１１ｆ　中間層
１１ｇ　内層
１２　液体流路
１２ａ　流路形成面
１３　蓋板
１３ａ　第１基材層
１３ｂ　強粘着層
１３ｃ　第２基材層
１３ｄ　弱粘着層
１４ｃ　計量槽
１５　第１凸部
１５ａ　第１凸部の頂部
１６　第２凸部
１６ａ　第２凸部の頂部
１７　スペーサ部材
１８　堰板
Ｓ１～Ｓ１４　開通部
Ｔ１～Ｔ２　閉止部
Ｐ１～Ｐ５、Ｐ１’　送液部
Ｇ１～Ｇ６　逆流防止部
１１０、１１０Ａ、１１０Ｂ　液体流路装置
１１１、１１１Ａ、１１１Ｂ　基板
１１１ａ、１４１ａ　基板の外層
１１１ｂ　基板の中間層
１１１ｃ、１４１ｄ　基板の内層
１４１ｂ　基板の外側中間層
１４１ｃ　基板の内側中間層
１１２　液体流路
１１２ａ　流路形成面
１１３、１１３Ａ　蓋板
１１３ａ　蓋板の外層
１１３ｂ　蓋板の内層
Ｓ１１～Ｓ１７　開通部
Ｔ１１　閉止部
１１５、１５０　栓体
１１６　封止材料供給槽
１３０　封止材料
１５１、１５２　凹部 10A, 10B Liquid channel device 11A, 11B Substrate 11e Outer layer 11f Intermediate layer 11g Inner layer 12 Liquid channel 12a Channel forming surface 13 Cover plate 13a First substrate layer 13b Strong adhesion layer 13c Second substrate layer 13d Weak adhesion layer 14c Measuring tank 15 First convex portion 15a First convex portion top 16 Second convex portion 16a Second convex portion top 17 Spacer member 18 Dam plate S1 to S14 Opening portion T1 to T2 Closing portion P1 to P5, P1 ′ Liquid part G1 to G6 Backflow prevention part 110, 110A, 110B Liquid flow path device 111, 111A, 111B Substrate 111a, 141a Substrate outer layer 111b Substrate intermediate layer 111c, 141d Substrate inner layer 141b Substrate outer intermediate layer 141c Inside of substrate Intermediate layer 112 Liquid channel 112a Channel formation surface 113, 113A Lid plate 113a Lid plate outer layer 113b Lid plate inner layers S11 to S17 Opening part T11 Closing part 115, 150 Plug body 116 Sealing material supply tank 130 Sealing material 151, 152 Recess

Claims (24)

　基板の少なくとも片面に、試料および試薬の少なくとも一方からなる液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、
　前記液槽の少なくとも１つは、該液槽内の液体を液槽外に送液する送液部を有し、
　該送液部は、前記液槽に対応する部分の蓋板または前記液槽の底部を外側から押圧する操作により作動する液体流路装置。 A liquid channel through which a liquid consisting of at least one of a sample and a reagent flows and at least one liquid tank in which the liquid is stored are formed on at least one surface of the substrate, and the liquid channel and the liquid tank of the substrate Is a liquid flow path device in which a cover plate is laminated on the flow path forming surface on which is formed,
At least one of the liquid tanks has a liquid feeding part for feeding the liquid in the liquid tank to the outside of the liquid tank,
The liquid feeding device is a liquid flow path device that is activated by an operation of pressing a lid plate corresponding to the liquid tank or a bottom of the liquid tank from the outside. 　前記液体流路の一部を閉止状態から開通状態にする開通部と、開通状態から閉止状態にする閉止部とをさらに有し、
　前記蓋板は、該蓋板の表面を構成する第１基材層と、該第１基材層の内側に形成された強粘着層と、該強粘着層の内側に形成された第２基材層と、該第２基材層の内側に形成され、前記流路形成面に粘着する弱粘着層とを有し、
　前記開通部では、前記液体流路に第１凸部が形成され、該第１凸部の頂部と前記弱粘着層とが粘着し、かつ、前記強粘着層と前記第２基材層とが離間し、
　前記閉止部では、前記液体流路に第２凸部が形成され、該第２凸部の頂部と前記弱粘着層とが離間し、かつ、前記強粘着層と前記第２基材層との間にはスペーサ部材が介在し、該スペーサ部材と前記強粘着層とが粘着し、
　前記送液部では、前記強粘着層と前記第２基材層との間にはスペーサ部材が介在し、該スペーサ部材と前記強粘着層とが粘着している請求項１に記載の液体流路装置。 An opening part that makes a part of the liquid flow path open from a closed state; and a closing part that makes the liquid flow path closed from the open state;
The lid plate includes a first base layer that constitutes a surface of the lid plate, a strong adhesive layer formed inside the first base layer, and a second base formed inside the strong adhesive layer. A material layer, and a weak adhesive layer that is formed inside the second base material layer and adheres to the flow path forming surface,
In the opening portion, a first convex portion is formed in the liquid channel, a top portion of the first convex portion and the weak adhesive layer are adhered, and the strong adhesive layer and the second base material layer are bonded to each other. Apart,
In the closing portion, a second convex portion is formed in the liquid channel, a top portion of the second convex portion and the weak adhesive layer are separated from each other, and the strong adhesive layer and the second base material layer are separated from each other. A spacer member is interposed between the spacer member and the strong adhesive layer,
2. The liquid flow according to claim 1, wherein a spacer member is interposed between the strong adhesion layer and the second base material layer in the liquid feeding section, and the spacer member and the strong adhesion layer are adhered to each other. Road equipment. 　前記基板は、外層と、該外層の内側に積層した中間層と、該中間層の内側に積層した内層とからなり、
　前記内層には、前記液槽の上部と、前記液体流路と、前記第１凸部と、前記第２凸部とが形成され、
　前記中間層には、前記液槽の下部が形成されている請求項２に記載の液体流路装置。 The substrate comprises an outer layer, an intermediate layer laminated inside the outer layer, and an inner layer laminated inside the intermediate layer,
In the inner layer, an upper portion of the liquid tank, the liquid flow path, the first convex portion, and the second convex portion are formed,
The liquid channel device according to claim 2, wherein a lower portion of the liquid tank is formed in the intermediate layer. 　前記基板は、外層と、該外層の内側に積層した内層とからなり、
　前記内層には、前記液槽と、前記液体流路と、前記第１凸部と、前記第２凸部とが形成されている請求項２に記載の液体流路装置。 The substrate comprises an outer layer and an inner layer laminated on the inner side of the outer layer,
The liquid channel device according to claim 2, wherein the liquid tank, the liquid channel, the first convex portion, and the second convex portion are formed in the inner layer. 　前記送液部が設けられた前記液槽には、該送液部で送液される液体の逆流を防止する逆流防止部が設けられている請求項１ないし４のいずれかに記載の液体流路装置。 The liquid flow according to any one of claims 1 to 4, wherein the liquid tank provided with the liquid feeding unit is provided with a backflow prevention unit for preventing a backflow of the liquid fed by the liquid feeding unit. Road equipment. 　前記送液部が設けられた前記液槽には、該送液部で送液される液体の逆流を防止する逆流防止部が設けられ、
　該逆流防止部は、前記内層に形成されている請求項３または４に記載の液体流路装置。 The liquid tank provided with the liquid feeding part is provided with a backflow prevention part for preventing the backflow of the liquid fed in the liquid feeding part,
The liquid channel device according to claim 3 or 4, wherein the backflow prevention unit is formed in the inner layer. 前記送液部は、前記液槽の底部を外側から押圧する操作により作動し、
前記底部は、外方に膨出して形成されている請求項１ないし６のいずれかに記載の液体流路装置。 The liquid feeding section is operated by an operation of pressing the bottom of the liquid tank from the outside,
The liquid channel device according to claim 1, wherein the bottom portion is formed to bulge outward. 　前記液体流路の一部を閉止状態から開通状態にする開通部を有し、
　前記蓋板は、該蓋板の表面を構成する第１基材層と、該第１基材層の内側に形成された強粘着層と、該強粘着層の内側に形成された第２基材層と、該第２基材層の内側に形成され、前記流路形成面に粘着する弱粘着層とを有し、
　前記開通部では、前記液体流路に第１凸部が形成され、該第１凸部の頂部と前記弱粘着層とが粘着し、かつ、前記強粘着層と前記第２基材層とが離間している請求項１に記載の液体流路装置。 Having an opening part for making a part of the liquid flow path from a closed state to an open state;
The lid plate includes a first base layer that constitutes a surface of the lid plate, a strong adhesive layer formed inside the first base layer, and a second base formed inside the strong adhesive layer. A material layer, and a weak adhesive layer that is formed inside the second base material layer and adheres to the flow path forming surface,
In the opening portion, a first convex portion is formed in the liquid channel, a top portion of the first convex portion and the weak adhesive layer are adhered, and the strong adhesive layer and the second base material layer are bonded to each other. The liquid channel device according to claim 1, which is spaced apart. 　前記液体流路には、一定量の液体を計量する計量槽が設けられ、少なくとも該計量槽の上流には前記閉止部が設けられ、下流には前記開通部が設けられている請求項２に記載の液体流路装置。 3. The liquid channel is provided with a measuring tank for measuring a certain amount of liquid, and at least upstream of the measuring tank, the closing part is provided, and downstream the opening part is provided. The liquid flow path device described. 　前記計量槽には、前記一定量を超えた液体をオーバーフローさせるオーバーフロー部が設けられている請求項９に記載の液体流路装置。 10. The liquid channel device according to claim 9, wherein the measuring tank is provided with an overflow portion for overflowing the liquid exceeding the predetermined amount. 　基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、
　前記液体流路の一部を閉止状態から開通状態にする開通部を有し、
　前記開通部は、前記液体流路の前記一部に配置され、前記蓋板または前記液体流路の底部を外側から押圧する操作により塑性変形し、前記開通状態とする栓体からなる液体流路装置。 A flow path forming surface in which a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is accumulated is formed on at least one surface of the substrate, and the liquid flow path and the liquid tank of the substrate are formed. Is a liquid flow path device in which a cover plate is laminated,
Having an opening part for making a part of the liquid flow path from a closed state to an open state;
The opening portion is disposed in the part of the liquid flow path, and is a liquid flow path formed of a plug body that is plastically deformed by an operation of pressing the lid plate or the bottom of the liquid flow path from outside. apparatus. 　前記蓋板または前記液体流路の底部における前記栓体と接する部分には、剥離処理が施されている請求項１１に記載の液体流路装置。 The liquid flow path device according to claim 11, wherein a part of the lid plate or the bottom portion of the liquid flow path that is in contact with the plug is subjected to a peeling process. 　請求項１１または１２に記載の液体流路装置の製造方法であって、
　前記基板に前記液体流路と前記液槽とを形成する第１工程と、前記液体流路の前記一部に前記栓体を形成する第２工程と、前記基板の前記流路形成面に前記蓋板を積層する第３工程とを有し、
　前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記内層を構成するシートと、前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層する液体流路装置の製造方法。 It is a manufacturing method of the liquid channel device according to claim 11 or 12,
A first step of forming the liquid flow path and the liquid tank on the substrate; a second step of forming the plug in the part of the liquid flow path; and A third step of laminating the lid plate,
In the first step, after forming the upper part of the liquid tank and the liquid flow path in the sheet constituting the inner layer of the substrate, and forming the lower part of the liquid tank in the sheet constituting the intermediate layer of the substrate, A method for manufacturing a liquid flow path device, in which a sheet constituting the inner layer, a sheet constituting the intermediate layer, and a sheet constituting the outer layer of the substrate are sequentially laminated. 　前記第２工程では、前記液体流路の前記一部に、前記栓体を形成するための栓体形成材料を塗布する方法により、前記栓体を形成する請求項１３に記載の液体流路装置の製造方法。 The liquid channel device according to claim 13, wherein, in the second step, the plug body is formed by a method of applying a plug body forming material for forming the plug body to the part of the liquid channel. Manufacturing method. 　基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、
　前記液体流路の一部を開通状態から閉止状態にする閉止部を有し、
　前記閉止部は、
　前記液体流路の前記一部から分岐して形成された封止材料供給槽と、
　該封止材料供給槽に充填され、該封止材料供給槽に対応する部分の蓋板または該封止材料供給槽の底部を外側から押圧する操作により、前記液体流路の前記一部に押し出され、前記閉止状態とする封止材料と、
　を有する液体流路装置。 A flow path forming surface in which a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is accumulated is formed on at least one surface of the substrate, and the liquid flow path and the liquid tank of the substrate are formed. Is a liquid flow path device in which a cover plate is laminated,
Having a closing portion for closing a part of the liquid flow path from the open state,
The closing portion is
A sealing material supply tank formed by branching from the part of the liquid channel;
The sealing material supply tank is filled and pushed into the part of the liquid channel by an operation of pressing the lid plate corresponding to the sealing material supply tank or the bottom of the sealing material supply tank from the outside. A sealing material to be in the closed state,
A liquid flow path device. 　請求項１５に記載の液体流路装置の製造方法であって、
　前記基板に前記液体流路と前記液槽と前記封止材料供給槽とを形成する第１工程と、前記封止材料供給槽に前記封止材料を充填する第２工程と、前記基板の前記流路形成面に前記蓋板を積層する第３工程とを有し、
　前記第１工程では、前記基板の内層を構成するシートに前記液槽の上部と前記液体流路と前記封止材料供給槽とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記内層を構成するシートと、前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層する液体流路装置の製造方法。 It is a manufacturing method of the liquid channel device according to claim 15,
A first step of forming the liquid channel, the liquid tank, and the sealing material supply tank on the substrate; a second step of filling the sealing material supply tank with the sealing material; A third step of laminating the lid plate on the flow path forming surface,
In the first step, an upper part of the liquid tank, the liquid channel, and the sealing material supply tank are formed on a sheet constituting the inner layer of the substrate, and the liquid tank is formed on the sheet constituting the intermediate layer of the substrate. After forming the lower part of this, the manufacturing method of the liquid flow-path apparatus which laminate | stacks the sheet | seat which comprises the said inner layer, the sheet | seat which comprises the said intermediate | middle layer, and the sheet | seat which comprises the outer layer of the said board | substrate sequentially. 　前記第２工程では、前記封止材料供給槽に前記封止材料を塗布する方法により、前記封止材料を充填する請求項１６に記載の液体流路装置の製造方法。 The method of manufacturing a liquid flow path device according to claim 16, wherein in the second step, the sealing material is filled by a method of applying the sealing material to the sealing material supply tank. 　基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、
　前記液体流路の一部を閉止状態から開通状態にする開通部を有し、
　前記開通部は、前記液体流路の前記一部に配置された栓体を具備し、
　前記蓋板の内面または前記液体流路の底部における前記栓体の対向位置には、前記栓体を収納可能な凹部が構成され、
　前記栓体は、前記蓋板または前記底部を外側から押圧する操作により、前記液体流路の前記一部から前記凹部内に移動して、前記開通状態とする液体流路装置。 A flow path forming surface in which a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is accumulated is formed on at least one surface of the substrate, and the liquid flow path and the liquid tank of the substrate are formed. Is a liquid flow path device in which a cover plate is laminated,
Having an opening part for making a part of the liquid flow path from a closed state to an open state;
The opening portion includes a stopper disposed in the part of the liquid flow path,
In the inner surface of the lid plate or at the bottom of the liquid flow path, a concavity that can store the plug is configured at a position facing the plug.
The plug body is a liquid channel device that moves from the part of the liquid channel into the recessed portion by an operation of pressing the lid plate or the bottom portion from the outside to enter the open state. 　請求項１８に記載の液体流路装置のうち、前記凹部が前記蓋板の前記内面に形成された液体流路装置の製造方法であって、
　前記基板に前記液体流路と前記液槽とを形成し、前記蓋板に前記凹部を形成する第１工程と、前記液体流路の前記一部に栓体を形成する第２工程と、前記基板の前記流路形成面に、前記蓋板を積層する第３工程とを有し、
　前記第１工程では、
　前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記基板の前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層して、前記基板に前記液体流路と前記液槽とを形成し、
　前記蓋板の内層を構成するシートに前記凹部を形成した後、前記蓋板の前記内層を構成するシートと、前記蓋板の外層を構成するシートとを積層して、前記蓋板に前記凹部を形成する液体流路装置の製造方法。 The liquid channel device according to claim 18, wherein the recess is formed on the inner surface of the lid plate.
A first step of forming the liquid channel and the liquid tank on the substrate, and forming the recess in the lid plate; a second step of forming a plug in the part of the liquid channel; A third step of laminating the lid plate on the flow path forming surface of the substrate,
In the first step,
The upper part of the liquid tank and the liquid channel are formed on a sheet constituting the inner layer of the substrate, and the lower part of the liquid tank is formed on a sheet constituting the intermediate layer of the substrate, and then the inner layer of the substrate is A sheet constituting the intermediate layer of the substrate and a sheet constituting the outer layer of the substrate are sequentially laminated to form the liquid flow path and the liquid tank on the substrate;
After forming the concave portion in the sheet constituting the inner layer of the lid plate, the sheet constituting the inner layer of the lid plate and the sheet constituting the outer layer of the lid plate are laminated, and the concave portion is formed on the lid plate. A method of manufacturing a liquid channel device that forms 　請求項１８に記載の液体流路装置のうち、前記凹部が前記液体流路の前記底部に形成された液体流路装置の製造方法であって、
　前記基板に前記液体流路と前記液槽と前記凹部を形成する第１工程と、前記蓋板の内面における前記凹部の対向位置に、栓体を形成する第２工程と、前記基板の前記流路形成面に、前記蓋板を積層する第３工程とを有し、
　前記第１工程では、
　前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の内側中間層を構成するシートに前記液槽の中間部と前記凹部を形成し、前記基板の外側中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記内側中間層を構成するシートと、前記外側中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層する液体流路装置の製造方法。 The liquid channel device according to claim 18, wherein the concave portion is formed on the bottom of the liquid channel.
A first step of forming the liquid channel, the liquid tank, and the recess in the substrate; a second step of forming a plug at a position opposite to the recess on the inner surface of the lid plate; and the flow of the substrate. A third step of laminating the cover plate on the path forming surface;
In the first step,
Forming an upper part of the liquid tank and the liquid flow path in a sheet constituting the inner layer of the substrate; forming an intermediate part of the liquid tank and the recess in a sheet constituting the inner intermediate layer of the substrate; After forming the lower part of the liquid tank on the sheet constituting the outer intermediate layer, the sheet constituting the inner layer of the substrate, the sheet constituting the inner intermediate layer, the sheet constituting the outer intermediate layer, A method of manufacturing a liquid flow path device in which sheets constituting an outer layer of the substrate are sequentially laminated. 　基板の少なくとも片面に、液体が流通する液体流路と、前記液体が溜まる１つ以上の液槽とが形成され、前記基板の前記液体流路と前記液槽とが形成された流路形成面には蓋板が積層した液体流路装置であって、
　前記液体流路の一部を開通状態から閉止状態にする閉止部を有し、
　前記閉止部は、前記蓋板の内面または前記液体流路の底部に形成された凹部内に収納された栓体を具備し、
　前記栓体は、前記蓋板または前記底部を外側から押圧する操作により前記凹部内から前記液体流路の前記一部に移動して、前記閉止状態とする液体流路装置。 A flow path forming surface in which a liquid flow path through which a liquid flows and at least one liquid tank in which the liquid is accumulated is formed on at least one surface of the substrate, and the liquid flow path and the liquid tank of the substrate are formed. Is a liquid flow path device in which a cover plate is laminated,
Having a closing portion for closing a part of the liquid flow path from the open state,
The closing portion includes a stopper housed in a recess formed in the inner surface of the lid plate or the bottom of the liquid channel,
The plug body is a liquid channel device that moves to the part of the liquid channel from the inside of the recess by an operation of pressing the lid plate or the bottom portion from the outside to be in the closed state. 　請求項２１に記載の液体流路装置のうち、前記凹部が前記蓋板の前記内面に形成された液体流路装置の製造方法であって、
　前記基板に前記液体流路と前記液槽とを形成し、前記蓋板に前記凹部を形成する第１工程と、前記凹部内に栓体を形成する第２工程と、前記基板の前記流路形成面に、前記蓋板を積層する第３工程とを有し、
　前記第１工程では、
　前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記基板の前記中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層して、前記基板に前記液体流路と前記液槽とを形成し、
　前記蓋板の内層を構成するシートに前記凹部を形成した後、前記蓋板の前記内層を構成するシートと、前記蓋板の外層を構成するシートとを積層して、前記蓋板に前記凹部を形成する液体流路装置の製造方法。 The liquid channel device according to claim 21, wherein the concave portion is formed on the inner surface of the lid plate.
A first step of forming the liquid channel and the liquid tank in the substrate and forming the recess in the lid plate; a second step of forming a plug in the recess; and the channel of the substrate. A third step of laminating the lid plate on the forming surface;
In the first step,
The upper part of the liquid tank and the liquid channel are formed on a sheet constituting the inner layer of the substrate, and the lower part of the liquid tank is formed on a sheet constituting the intermediate layer of the substrate, and then the inner layer of the substrate is A sheet constituting the intermediate layer of the substrate and a sheet constituting the outer layer of the substrate are sequentially laminated to form the liquid flow path and the liquid tank on the substrate;
After forming the concave portion in the sheet constituting the inner layer of the lid plate, the sheet constituting the inner layer of the lid plate and the sheet constituting the outer layer of the lid plate are laminated, and the concave portion is formed on the lid plate. A method of manufacturing a liquid channel device that forms 　請求項２１に記載の液体流路装置のうち、前記凹部が前記液体流路の底部に形成された液体流路装置の製造方法であって、
　前記基板に前記液体流路と前記液槽と前記凹部を形成する第１工程と、前記凹部内に栓体を形成する第２工程と、前記基板の前記流路形成面に前記蓋板を積層する第３工程とを有し、
　前記第１工程では、
　前記基板の内層を構成するシートに前記液槽の上部と前記液体流路とを形成し、前記基板の内側中間層を構成するシートに前記液槽の中間部と前記凹部を形成し、前記基板の外側中間層を構成するシートに前記液槽の下部を形成した後、前記基板の前記内層を構成するシートと、前記内側中間層を構成するシートと、前記外側中間層を構成するシートと、前記基板の外層を構成するシートとを順次積層する液体流路装置の製造方法。 The liquid channel device according to claim 21, wherein the concave portion is formed at the bottom of the liquid channel.
A first step of forming the liquid channel, the liquid tank, and the recess in the substrate; a second step of forming a plug in the recess; and laminating the cover plate on the channel forming surface of the substrate And a third step to
In the first step,
Forming an upper part of the liquid tank and the liquid flow path in a sheet constituting the inner layer of the substrate; forming an intermediate part of the liquid tank and the recess in a sheet constituting the inner intermediate layer of the substrate; After forming the lower part of the liquid tank on the sheet constituting the outer intermediate layer, the sheet constituting the inner layer of the substrate, the sheet constituting the inner intermediate layer, the sheet constituting the outer intermediate layer, A method of manufacturing a liquid flow path device in which sheets constituting an outer layer of the substrate are sequentially laminated. 　前記第２工程では、前記栓体を形成するための栓体形成材料を塗布する方法により、前記栓体を形成する請求項１９，２０，２２，２３のいずれかに記載の液体流路装置の製造方法。 The liquid channel device according to any one of claims 19, 20, 22, and 23, wherein, in the second step, the plug is formed by a method of applying a plug forming material for forming the plug. Production method.
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